WO2000078957A3 - Scorpion toxins from buthotus judaicus - Google Patents

Scorpion toxins from buthotus judaicus Download PDF

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Publication number
WO2000078957A3
WO2000078957A3 PCT/US2000/017048 US0017048W WO0078957A3 WO 2000078957 A3 WO2000078957 A3 WO 2000078957A3 US 0017048 W US0017048 W US 0017048W WO 0078957 A3 WO0078957 A3 WO 0078957A3
Authority
WO
WIPO (PCT)
Prior art keywords
scorpion toxins
channel
relates
chimeric gene
buthotus judaicus
Prior art date
Application number
PCT/US2000/017048
Other languages
French (fr)
Other versions
WO2000078957A2 (en
Inventor
Rafael Herrmann
Jian-Ming Lee
James F Wong
Original Assignee
Du Pont
Rafael Herrmann
Lee Jian Ming
James F Wong
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Rafael Herrmann, Lee Jian Ming, James F Wong filed Critical Du Pont
Priority to CA002372701A priority Critical patent/CA2372701A1/en
Priority to AU57542/00A priority patent/AU5754200A/en
Priority to DE60040067T priority patent/DE60040067D1/de
Priority to EP00943005A priority patent/EP1185653B1/en
Publication of WO2000078957A2 publication Critical patent/WO2000078957A2/en
Publication of WO2000078957A3 publication Critical patent/WO2000078957A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43522—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from scorpions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/14011—Baculoviridae
    • C12N2710/14111—Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Insects & Arthropods (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

This invention relates to an isolated nucleic acid fragment encoding scorpion toxins that are Na-channel modifiers. The invention also relates to the construction of a chimeric gene encoding all or a substantial portion of the Na-channel modifier, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the Na-channel modifier in a transformed host cell.
PCT/US2000/017048 1999-06-22 2000-06-21 Scorpion toxins from buthotus judaicus WO2000078957A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002372701A CA2372701A1 (en) 1999-06-22 2000-06-21 Scorpion toxins
AU57542/00A AU5754200A (en) 1999-06-22 2000-06-21 Scorpion toxins from buthotus judaicus
DE60040067T DE60040067D1 (en) 1999-06-22 2000-06-21
EP00943005A EP1185653B1 (en) 1999-06-22 2000-06-21 Scorpion toxins from buthotus judaicus

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14041099P 1999-06-22 1999-06-22
US60/140,410 1999-06-22

Publications (2)

Publication Number Publication Date
WO2000078957A2 WO2000078957A2 (en) 2000-12-28
WO2000078957A3 true WO2000078957A3 (en) 2001-05-03

Family

ID=22491107

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/017048 WO2000078957A2 (en) 1999-06-22 2000-06-21 Scorpion toxins from buthotus judaicus

Country Status (8)

Country Link
EP (1) EP1185653B1 (en)
AR (1) AR024435A1 (en)
AT (1) ATE406442T1 (en)
AU (1) AU5754200A (en)
CA (1) CA2372701A1 (en)
DE (1) DE60040067D1 (en)
ES (1) ES2312349T3 (en)
WO (1) WO2000078957A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002330245A1 (en) 2001-10-04 2003-04-14 The Regents Of The University Of California Isolated polypeptides and compositions from the venom of p. transvaalicus and methods of use
US7414173B1 (en) 2002-07-12 2008-08-19 E.I. Du Pont De Nemours And Company Isolated nucleic acid molecules encoding orally active androctonus amoreuxi pesticidal biopeptides
IL155972A0 (en) * 2003-05-19 2003-12-23 Yissum Res Dev Co Cardiotonic polypeptides
CN110055315A (en) * 2019-03-20 2019-07-26 中山大学 The DNA molecular discrimination method of scorpio medicinal material in a kind of cerebral ischemic preparation

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992016637A1 (en) * 1991-03-22 1992-10-01 Roussel-Uclaf Biological control agents
WO1996036221A2 (en) * 1995-05-08 1996-11-21 The Regents Of The University Of California Insect control with multiple toxins
WO1996036712A1 (en) * 1995-05-17 1996-11-21 E.I. Du Pont De Nemours And Company Recombinant baculovirus insecticides
WO2000024772A2 (en) * 1998-10-23 2000-05-04 E.I. Du Pont De Nemours And Company Scorpion toxins

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992016637A1 (en) * 1991-03-22 1992-10-01 Roussel-Uclaf Biological control agents
WO1996036221A2 (en) * 1995-05-08 1996-11-21 The Regents Of The University Of California Insect control with multiple toxins
WO1996036712A1 (en) * 1995-05-17 1996-11-21 E.I. Du Pont De Nemours And Company Recombinant baculovirus insecticides
WO2000024772A2 (en) * 1998-10-23 2000-05-04 E.I. Du Pont De Nemours And Company Scorpion toxins

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FROY OREN ET AL: "The putative bioactive surface of insect-selective scorpion excitatory neurotoxins.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 9, 26 February 1999 (1999-02-26), pages 5769 - 5776, XP002149679, ISSN: 0021-9258 *
LORET E P ET AL: "AN ANTI-INSECT TOXIN PURIFIED FROM THE SCORPION ANDROCTONUS-AUSTRALIS HECTOR ALSO ACTS ON THE ALPHA AND BETA-SITES OF THE MAMMALIAN SODIUM CHANNEL SEQUENCE AND CIRCULAR DICHROISM STUDY", BIOCHEMISTRY, vol. 30, no. 3, 1991, pages 633 - 640, XP002149680, ISSN: 0006-2960 *

Also Published As

Publication number Publication date
EP1185653B1 (en) 2008-08-27
CA2372701A1 (en) 2000-12-28
AR024435A1 (en) 2002-10-02
ATE406442T1 (en) 2008-09-15
DE60040067D1 (en) 2008-10-09
EP1185653A2 (en) 2002-03-13
WO2000078957A2 (en) 2000-12-28
ES2312349T3 (en) 2009-03-01
AU5754200A (en) 2001-01-09

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