WO2000075352A3 - Hcv/bvdv chimeric genomes and uses thereof - Google Patents

Hcv/bvdv chimeric genomes and uses thereof Download PDF

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Publication number
WO2000075352A3
WO2000075352A3 PCT/US2000/015527 US0015527W WO0075352A3 WO 2000075352 A3 WO2000075352 A3 WO 2000075352A3 US 0015527 W US0015527 W US 0015527W WO 0075352 A3 WO0075352 A3 WO 0075352A3
Authority
WO
WIPO (PCT)
Prior art keywords
hcv
chimeric
bvdv
genomes
nucleic acid
Prior art date
Application number
PCT/US2000/015527
Other languages
French (fr)
Other versions
WO2000075352A2 (en
Inventor
Jae-Hwan Nam
Jens Bukh
Suzanne U Emerson
Robert H Purcell
Original Assignee
Us Health
Nam Jae Hwan
Jens Bukh
Suzanne U Emerson
Robert H Purcell
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US10/009,011 priority Critical patent/US7009044B1/en
Application filed by Us Health, Nam Jae Hwan, Jens Bukh, Suzanne U Emerson, Robert H Purcell filed Critical Us Health
Priority to AU53245/00A priority patent/AU5324500A/en
Priority to EP00938165A priority patent/EP1187927A2/en
Publication of WO2000075352A2 publication Critical patent/WO2000075352A2/en
Publication of WO2000075352A3 publication Critical patent/WO2000075352A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24311—Pestivirus, e.g. bovine viral diarrhea virus
    • C12N2770/24322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24311—Pestivirus, e.g. bovine viral diarrhea virus
    • C12N2770/24341—Use of virus, viral particle or viral elements as a vector
    • C12N2770/24344—Chimeric viral vector comprising heterologous viral elements for production of another viral vector

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

The present invention relates to molecular approaches to the production of nucleic acid sequences which comprise the genomes of chimeric hepatitis C virus-bovine viral diarrhea viruses (HCV-BVDV). The invention also relates to the use of these chimeric nucleic acid sequences to produce chimeric virions in cells and the use of these chimeric virions in HCV antibody neutralization assays, and for the development of vaccines and therapeutics for HCV.
PCT/US2000/015527 1999-06-04 2000-06-02 Hcv/bvdv chimeric genomes and uses thereof WO2000075352A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/009,011 US7009044B1 (en) 1999-06-04 2000-02-06 HCV/BVDV chimeric genomes and uses thereof
AU53245/00A AU5324500A (en) 1999-06-04 2000-06-02 Hcv/bvdv chimeric genomes and uses thereof
EP00938165A EP1187927A2 (en) 1999-06-04 2000-06-02 Hcv/bvdv chimeric genomes and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13781799P 1999-06-04 1999-06-04
US60/137,817 1999-06-04

Publications (2)

Publication Number Publication Date
WO2000075352A2 WO2000075352A2 (en) 2000-12-14
WO2000075352A3 true WO2000075352A3 (en) 2001-11-15

Family

ID=22479160

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/015527 WO2000075352A2 (en) 1999-06-04 2000-06-02 Hcv/bvdv chimeric genomes and uses thereof

Country Status (3)

Country Link
EP (1) EP1187927A2 (en)
AU (1) AU5324500A (en)
WO (1) WO2000075352A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103924006A (en) * 2014-04-21 2014-07-16 江苏省农业科学院 Primer sequence for detecting nucleotide fragment of HoBi-like pestivirus

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE430798T1 (en) 2002-09-13 2009-05-15 Inst Nat Sante Rech Med INFECTIOUS HEPACIVIRUS PSEUDOPARTICLES WITH FUNCTIONAL E1, E2 ENVELOPE PROTEINS
US20100129902A1 (en) * 2008-11-24 2010-05-27 Erhard Ralf Schoenbrunner Replication Stable and RNase Resistant Chimeras of Pestivirus with Insertion in 3' Nontranslated Region (3'NTR)
US9775894B2 (en) 2013-07-09 2017-10-03 University Of Washington Through Its Center For Commercialization Methods and compositions for activation of innate immune responses through RIG-I like receptor signaling

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999055366A1 (en) * 1998-04-24 1999-11-04 Washington University Chimeras of hepatitis c virus and bovine viral diarrhea virus

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999055366A1 (en) * 1998-04-24 1999-11-04 Washington University Chimeras of hepatitis c virus and bovine viral diarrhea virus

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
FROLOV I ET AL: "CIS-ACTING RNA ELEMENTS REQUIRED FOR REPLICATION OF BOVINE VIRAL DIARRHEA VIRUS-HEPATITIS C VIRUS 5' NONTRANSLATED REGION CHIMERAS", RNA,CAMBRIGDE UNIVERSITY PRESS, CAMBRIDGE,GB, vol. 4, no. 11, 25 November 1998 (1998-11-25), pages 1418 - 1435, XP000952790, ISSN: 1355-8382 *
H LU ET AL: "Poliovirus chimeras replicating under the translation control of genetic elements of HCV reveal unusual properties of the IRES of HCV", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,NATIONAL ACADEMY OF SCIENCE. WASHINGTON,US, vol. 93, February 1996 (1996-02-01), pages 1412 - 1417, XP002919370, ISSN: 0027-8424 *
LAI VC, ZHONG W, SKELTON A, INGRAVALLO P, VASSILEV V, DONIS RO, HONG Z, LAU JY: "Generation and characterization of a hepatitis C virus NS3 protease-dependent bovine viral diarrhea virus.", JOURNAL OF VIROLOGY., vol. 74, no. 14, July 2000 (2000-07-01), THE AMERICAN SOCIETY FOR MICROBIOLOGY., US, pages 6339 - 6347, XP000952808, ISSN: 0022-538X *
MEYERS G ET AL: "RECOVERY OF CYTOPATHOGENIC AND NONCYTOPATHOGENIC BOVINE VIRAL DIARRHEA VIRUSES FROM CDNA CONSTRUCTS", JOURNAL OF VIROLOGY,THE AMERICAN SOCIETY FOR MICROBIOLOGY,US, vol. 70, no. 12, December 1996 (1996-12-01), pages 8606 - 8613, XP000952807, ISSN: 0022-538X *
VENUGOPAL K. & GOULD E.A.: "Towards a new generation of Flavivirus vaccines", VACCINE, vol. 2, no. 11, 1994, GUILDFORD GB, pages 966 - 975, XP002919372 *
YU H ET AL: "SEQUENCE AND STRUCTURAL ELEMENTS AT THE 3' TERMINUS OF BOVINE VIRALDIARRHEA VIRUS GENOMIC RNA: FUNCTIONAL ROLE DURING RNA REPLICATION", JOURNAL OF VIROLOGY,THE AMERICAN SOCIETY FOR MICROBIOLOGY,US, vol. 73, no. 5, May 1999 (1999-05-01), pages 3638 - 3648, XP000946998, ISSN: 0022-538X *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103924006A (en) * 2014-04-21 2014-07-16 江苏省农业科学院 Primer sequence for detecting nucleotide fragment of HoBi-like pestivirus

Also Published As

Publication number Publication date
EP1187927A2 (en) 2002-03-20
WO2000075352A2 (en) 2000-12-14
AU5324500A (en) 2000-12-28

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