WO2000073797A3 - Homogeneous tests for sequentially determining lipoprotein fractions - Google Patents

Homogeneous tests for sequentially determining lipoprotein fractions Download PDF

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Publication number
WO2000073797A3
WO2000073797A3 PCT/US2000/014827 US0014827W WO0073797A3 WO 2000073797 A3 WO2000073797 A3 WO 2000073797A3 US 0014827 W US0014827 W US 0014827W WO 0073797 A3 WO0073797 A3 WO 0073797A3
Authority
WO
WIPO (PCT)
Prior art keywords
cholesterol
sample
complexed
lipoprotein fraction
fraction
Prior art date
Application number
PCT/US2000/014827
Other languages
French (fr)
Other versions
WO2000073797A2 (en
WO2000073797A9 (en
Inventor
Alan T Remaley
Maureen L Sampson
Gyorgy Csako
Original Assignee
Us Gov Health & Human Serv
Alan T Remaley
Maureen L Sampson
Gyorgy Csako
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Gov Health & Human Serv, Alan T Remaley, Maureen L Sampson, Gyorgy Csako filed Critical Us Gov Health & Human Serv
Priority to EP00939404A priority Critical patent/EP1183535A2/en
Priority to CA002375210A priority patent/CA2375210A1/en
Priority to JP2001500866A priority patent/JP2003501630A/en
Priority to AU54493/00A priority patent/AU771951B2/en
Publication of WO2000073797A2 publication Critical patent/WO2000073797A2/en
Publication of WO2000073797A3 publication Critical patent/WO2000073797A3/en
Publication of WO2000073797A9 publication Critical patent/WO2000073797A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders
    • G01N2800/044—Hyperlipemia or hypolipemia, e.g. dyslipidaemia, obesity

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Hematology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention provides new homogeneous assays for the determination of the amount of LDL-C, of HDL-C, and of total cholesterol present in a sample. The method comprises complexing a first lipoprotein fraction with a complex-forming agent, such as an antibody, using enzymes to detect cholesterol in the non-complexed lipoprotein fraction, measuring the amount of cholesterol in the non-complexed fraction to provide a first cholesterol value, and then dissociating the complexed lipoprotein fraction from the complex-forming agent so that that cholesterol is available to be a substrate for the enzymes. The total amount of cholesterol present in the sample can then be determined. Further, the first cholesterol value obtained can be subtracted from the total cholesterol to obtain a value for the first lipoprotein fraction present in the sample. Optionally, a triglyceride assay can then also be performed on the sample in the same tube.
PCT/US2000/014827 1999-05-28 2000-05-26 Homogeneous tests for sequentially determining lipoprotein fractions WO2000073797A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP00939404A EP1183535A2 (en) 1999-05-28 2000-05-26 Homogeneous tests for sequentially determining lipoprotein fractions
CA002375210A CA2375210A1 (en) 1999-05-28 2000-05-26 Homogeneous tests for sequentially determining lipoprotein fractions
JP2001500866A JP2003501630A (en) 1999-05-28 2000-05-26 Simultaneous test for continuous measurement of lipoprotein fraction
AU54493/00A AU771951B2 (en) 1999-05-28 2000-05-26 Homogeneous tests for sequentially determining lipoprotein fractions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13670999P 1999-05-28 1999-05-28
US60/136,709 1999-05-28

Publications (3)

Publication Number Publication Date
WO2000073797A2 WO2000073797A2 (en) 2000-12-07
WO2000073797A3 true WO2000073797A3 (en) 2001-09-13
WO2000073797A9 WO2000073797A9 (en) 2002-02-21

Family

ID=22474024

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/014827 WO2000073797A2 (en) 1999-05-28 2000-05-26 Homogeneous tests for sequentially determining lipoprotein fractions

Country Status (5)

Country Link
EP (1) EP1183535A2 (en)
JP (1) JP2003501630A (en)
AU (1) AU771951B2 (en)
CA (1) CA2375210A1 (en)
WO (1) WO2000073797A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7939283B2 (en) 2001-11-01 2011-05-10 Fisher Scientific Company L.L.C. Analyte binding turbidity assay
AU2003289081B2 (en) * 2002-12-13 2009-11-19 Denka Company Limited Method of multiple quantification of cholesterol in low-density lipoproteins
US7625721B2 (en) 2004-02-03 2009-12-01 Polymer Technology Systems, Inc. Non-precipitating bodily fluid analysis system
US7435577B2 (en) 2004-02-03 2008-10-14 Polymer Technology Systems, Inc. Direct measurement of chlolesterol from low density lipoprotein with test strip
JP4647927B2 (en) * 2004-03-31 2011-03-09 デンカ生研株式会社 Multiple determination of cholesterol in low density lipoprotein
WO2006023678A1 (en) * 2004-08-17 2006-03-02 Polymer Technology Systems, Inc. Non-precipitating bodily fluid analysis system
US20060062690A1 (en) 2004-08-17 2006-03-23 Polymer Technology Systems, Inc. Apparatus and method of manufacturing bodily fluid test strip
CN100365409C (en) * 2005-06-01 2008-01-30 王贤理 Reagent for measuring low-density lipoproteincholesterol and preparation method
US20090181413A1 (en) * 2005-09-30 2009-07-16 Denka Seiken Co., Ltd. Simultaneous and differential quantification of two target analytes in biological sample
GB0526051D0 (en) * 2005-12-21 2006-02-01 Oxford Biosensors Ltd Cholesterol sensor
CN101078729B (en) * 2006-05-10 2012-07-18 富士胶片株式会社 Method for measuring high density lipoprotein cholesterol
CN105044364B (en) * 2015-05-02 2016-09-14 王贤俊 LDL-C detection kit prepared by the method for covering is combined in a kind of employing
EP3320345A4 (en) * 2015-07-07 2018-11-07 Mohmed E. Ashmaig Methods of determining a high density lipoprotein phospholipid level in a sample
CN113631272B (en) 2019-03-19 2022-12-20 美国西门子医学诊断股份有限公司 Compositions, devices and methods for mitigating lipoprotein interference in vitro diagnostic assays for hydrophobic analytes
CN111893163B (en) * 2020-08-06 2023-01-17 武汉生之源生物科技股份有限公司 High-density lipoprotein 3 cholesterol detection kit, preparation method and application
CN112114127A (en) * 2020-09-09 2020-12-22 武汉生之源生物科技股份有限公司 Glycocholic acid homogeneous enzyme immunoassay kit and preparation method and application thereof
CN112695071B (en) * 2020-12-16 2022-12-30 浙江伊利康生物技术有限公司 High-density lipoprotein 3 determination reagent, method and kit

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0754948A1 (en) * 1995-07-21 1997-01-22 Wako Pure Chemical Industries, Ltd Method for measuring the amount of constituent contained in specific lipotrotein
US5814472A (en) * 1997-05-13 1998-09-29 Wako Pure Chemical Industries, Ltd. Measurement of LDL-cholesterol

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0754948A1 (en) * 1995-07-21 1997-01-22 Wako Pure Chemical Industries, Ltd Method for measuring the amount of constituent contained in specific lipotrotein
US5814472A (en) * 1997-05-13 1998-09-29 Wako Pure Chemical Industries, Ltd. Measurement of LDL-cholesterol

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HALLORAN, ROETERING ET AL: "Reference standardization and analytical performance of a liquid homogeneous high-density lipoprotein cholesterol method compared with chemical precipitation method", ARCH PATHOL LAB MED, vol. 123, April 1999 (1999-04-01), pages 317 - 326, XP000985566 *
NAUCK ET AL: "New immunoseparation-based homogeneous assay for HDL-cholesterol compared with three homogeneous and two heterogeneous methods for HDL-cholesterol", CLINICAL CHEMISTRY, vol. 44, no. 7, 1998, pages 1443 - 1451, XP000985308 *

Also Published As

Publication number Publication date
WO2000073797A2 (en) 2000-12-07
WO2000073797A9 (en) 2002-02-21
AU771951B2 (en) 2004-04-08
AU5449300A (en) 2000-12-18
JP2003501630A (en) 2003-01-14
EP1183535A2 (en) 2002-03-06
CA2375210A1 (en) 2000-12-07

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