WO2000073784A3 - Devices and methods for assessing cellular transport for research, drug discovery and testing, clinical diagnoses, and therapy design - Google Patents

Devices and methods for assessing cellular transport for research, drug discovery and testing, clinical diagnoses, and therapy design Download PDF

Info

Publication number
WO2000073784A3
WO2000073784A3 PCT/US2000/014805 US0014805W WO0073784A3 WO 2000073784 A3 WO2000073784 A3 WO 2000073784A3 US 0014805 W US0014805 W US 0014805W WO 0073784 A3 WO0073784 A3 WO 0073784A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
drug
efflux
research
testing
Prior art date
Application number
PCT/US2000/014805
Other languages
French (fr)
Other versions
WO2000073784A2 (en
Inventor
Miklos Gratzl
Hongwen Lu
Takashi Matsumoto
Chen Yi
Gary R Bright
Rohit Kashyap
Original Assignee
Univ Case Western Reserve
Miklos Gratzl
Hongwen Lu
Takashi Matsumoto
Chen Yi
Gary R Bright
Rohit Kashyap
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Case Western Reserve, Miklos Gratzl, Hongwen Lu, Takashi Matsumoto, Chen Yi, Gary R Bright, Rohit Kashyap filed Critical Univ Case Western Reserve
Priority to AU53029/00A priority Critical patent/AU5302900A/en
Priority to EP00937916A priority patent/EP1190246A2/en
Publication of WO2000073784A2 publication Critical patent/WO2000073784A2/en
Publication of WO2000073784A3 publication Critical patent/WO2000073784A3/en
Priority to US10/682,168 priority patent/US7521020B2/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp

Abstract

A method of measuring efflux/influx of a chemical drug (26), such as doxorubicin from a cell (14, 52), such as a human cancer cell or other biological cell, includes introducing the drug to the cell, for example by immersing the cell in a culture medium containing the drug or by direct injection into the cell. The cell containing the drug is then immersed in a drug-free liquid medium (28) and the efflux/influx of the drug from the cell measured with electrochemical equipment (60). For detection of efflux from small populations of cells, or a single cell, the signal strength may be increased by preconcentrating the drug on a working electrode (20, 50) and by increasing the oxygen concentration of the medium around the cell. A cell or small group of cells (52) to be studied may be spatially positioned on a substrate (58) by coating a small area (72) of the substrate with a layer (73) of a hydrophilic material to which the cell attaches.
PCT/US2000/014805 1999-05-28 2000-05-30 Devices and methods for assessing cellular transport for research, drug discovery and testing, clinical diagnoses, and therapy design WO2000073784A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU53029/00A AU5302900A (en) 1999-05-28 2000-05-30 Devices and methods for assessing cellular transport for research, drug discovery and testing, clinical diagnoses, and therapy design
EP00937916A EP1190246A2 (en) 1999-05-28 2000-05-30 Devices and methods for assessing cellular transport for research, drug discovery and testing, clinical diagnoses, and therapy design
US10/682,168 US7521020B2 (en) 1999-05-28 2003-10-09 Device for precise chemical delivery and solution preparation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13713499P 1999-05-28 1999-05-28
US60/137,134 1999-05-28

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09980089 A-371-Of-International 2003-06-26
US10/682,168 Continuation-In-Part US7521020B2 (en) 1999-05-28 2003-10-09 Device for precise chemical delivery and solution preparation

Publications (2)

Publication Number Publication Date
WO2000073784A2 WO2000073784A2 (en) 2000-12-07
WO2000073784A3 true WO2000073784A3 (en) 2001-07-19

Family

ID=22475976

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/014805 WO2000073784A2 (en) 1999-05-28 2000-05-30 Devices and methods for assessing cellular transport for research, drug discovery and testing, clinical diagnoses, and therapy design

Country Status (3)

Country Link
EP (1) EP1190246A2 (en)
AU (1) AU5302900A (en)
WO (1) WO2000073784A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10202887B4 (en) * 2002-01-25 2004-05-06 Advalytix Ag Cell analysis method
US10591462B2 (en) 2016-03-03 2020-03-17 Mohammad Abdolahad Electrochemical method and device for detecting the effect of anticancer drugs

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4128456A (en) * 1977-10-11 1978-12-05 Lee Kai S Suction electrode for intracellular potential measurement
US4901446A (en) * 1988-04-20 1990-02-20 Narishige Scientific Instrument Laboratory, Ltd. Apparatus for finely actuating a glass electrode or the like
US5278048A (en) * 1988-10-21 1994-01-11 Molecular Devices Corporation Methods for detecting the effect of cell affecting agents on living cells
US5496697A (en) * 1988-10-21 1996-03-05 Molecular Devices Corporation Methods and apparatus for detecting the effect of cell affecting agents on living cells
WO1998007034A1 (en) * 1996-08-14 1998-02-19 Technion Research And Development Foundation Ltd. Assay for multidrug resistance
US5759846A (en) * 1995-04-12 1998-06-02 Chemodyne S.A. Device for the study of organotypic cultures and its uses in electrophysiology and biochemistry
WO1998055870A1 (en) * 1997-06-04 1998-12-10 A+ Science Invest Ab Detection of biologically active molecules by use of pre-activated cell-based biosensors in liquid-based separation systems
DE19744649A1 (en) * 1997-10-09 1999-04-15 Fraunhofer Ges Forschung Patch clamp cell measurement apparatus, has an array of micro dishes containing cells, and an assembly of micro pipettes with relative movements between them

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4128456A (en) * 1977-10-11 1978-12-05 Lee Kai S Suction electrode for intracellular potential measurement
US4901446A (en) * 1988-04-20 1990-02-20 Narishige Scientific Instrument Laboratory, Ltd. Apparatus for finely actuating a glass electrode or the like
US5278048A (en) * 1988-10-21 1994-01-11 Molecular Devices Corporation Methods for detecting the effect of cell affecting agents on living cells
US5496697A (en) * 1988-10-21 1996-03-05 Molecular Devices Corporation Methods and apparatus for detecting the effect of cell affecting agents on living cells
US5759846A (en) * 1995-04-12 1998-06-02 Chemodyne S.A. Device for the study of organotypic cultures and its uses in electrophysiology and biochemistry
WO1998007034A1 (en) * 1996-08-14 1998-02-19 Technion Research And Development Foundation Ltd. Assay for multidrug resistance
WO1998055870A1 (en) * 1997-06-04 1998-12-10 A+ Science Invest Ab Detection of biologically active molecules by use of pre-activated cell-based biosensors in liquid-based separation systems
DE19744649A1 (en) * 1997-10-09 1999-04-15 Fraunhofer Ges Forschung Patch clamp cell measurement apparatus, has an array of micro dishes containing cells, and an assembly of micro pipettes with relative movements between them

Also Published As

Publication number Publication date
EP1190246A2 (en) 2002-03-27
AU5302900A (en) 2000-12-18
WO2000073784A2 (en) 2000-12-07

Similar Documents

Publication Publication Date Title
EP0079502B1 (en) Multilayer enzyme electrode membrane, method of making same and polarographic cell structure
Mǎdǎraş et al. Miniaturized biosensors employing electropolymerized permselective films and their use for creatinine assays in human serum
US3979274A (en) Membrane for enzyme electrodes
US6852525B1 (en) Mammalian cell culture chamber
JPH03505783A (en) Biological fluid measuring device
EP0625883A1 (en) Acrylic copolymer membranes for biosensors
CA2259984A1 (en) Precise efficacy assay methods for active agents including chemotherapeutic agents
WO2001021827A1 (en) Small volume biosensor for continuous analyte monitoring
JPH11512316A (en) Chemical signal impervious mask
JP2002541441A (en) Analytical device for continuous measurement of fluid properties
JPS58193452A (en) Method of measuring lactic acid or its derivative
EP2909606B1 (en) Device and methods of using device for detection of aminoacidopathies
EP3747364A1 (en) Protective film material for biosensor probe
EP1277054B1 (en) Electrochemical sensing
EP1085098A3 (en) A composition for providing long term stability to cells for diagnostic testing
CN102428364A (en) Membrane System With Sufficient Buffering Capacity
Kimura et al. A novel blood glucose monitoring method an ISFET biosensor applied to transcutaneous effusion fluid
Tang et al. Composite liquid membrane for enzyme electrode construction
WO2000073784A3 (en) Devices and methods for assessing cellular transport for research, drug discovery and testing, clinical diagnoses, and therapy design
Lubrano et al. Amperometric alcohol electrode with extended linearity and reduced interferences
CN105842315B (en) A kind of electrode sensitive layer for biochemical oxygen demand (BOD) detection
EP1106683A3 (en) A method for providing long term stability to cells for diagnostic testing
CA1215660A (en) Enzyme electrode membrane wherein enzyme is protectively encapsulated
Erfle et al. Lysosomal enzyme release from human neutrophils adherent to foreign material surfaces: Enhanced release of elastase activity
Naware et al. Design and microfabrication of a polymer-modified carbon sensor array for the measurement of neurotransmitter signals

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM DZ EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

WWE Wipo information: entry into national phase

Ref document number: 2000937916

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2000937916

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Ref document number: 2000937916

Country of ref document: EP