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Microscale diffusion immunoassay

Info

Publication number
WO2000072020A8
WO2000072020A8 PCT/US2000/013864 US0013864W WO2000072020A8 WO 2000072020 A8 WO2000072020 A8 WO 2000072020A8 US 0013864 W US0013864 W US 0013864W WO 2000072020 A8 WO2000072020 A8 WO 2000072020A8
Authority
WO
Grant status
Application
Patent type
Prior art keywords
diffusion
particles
concentration
analyte
immunoassay
Prior art date
Application number
PCT/US2000/013864
Other languages
French (fr)
Other versions
WO2000072020A1 (en )
WO2000072020A9 (en )
Inventor
Bernhard H Weigl
Paul Yager
Andrew Kamholz
Anson Hatch
Original Assignee
Univ Washington
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay
    • G01N33/558Immunoassay; Biospecific binding assay using diffusion or migration of antigen or antibody
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/0005Field flow fractionation

Abstract

Methods and apparatuses are provided for determining presence and concentration of analytes by exploiting molecular binding reactions and differential diffusion rates. Analyte particles and binding particles are allowed to diffuse toward each other, and slowing of the diffusion front is detected when they meet. From the position of the diffusion front, presence and concentration of analyte particles can be determined. One embodiment provides a competitive immunoassay in a microfluidic format. This diffusion immunoassay (DIA) relies on measuring the concentration of labeled antigen along one dimension of a microchannel after allowing it to diffuse for a short time into a region containing specific antibodies. A simple microfluidic device, the T-Sensor, was used to implement a DIA to measure the concentration of phenytoin, a small drug molecule. Concentrations of analyte over the range of 50 to 1600 nM can be measured in less than a minute. The assay is homogenous, rapid, requires only microliter volumes of reagents and sample, and is applicable to a wide range of analytes, including therapeutic drugs, molecular biological markers, and environmental contaminants. Methods for separating particles of similar size in a diffusion separator are also provided.
PCT/US2000/013864 1999-05-21 2000-05-19 Microscale diffusion immunoassay WO2000072020A9 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US13541799 true 1999-05-21 1999-05-21
US09503563 US20020090644A1 (en) 1999-05-21 2000-02-14 Microscale diffusion immunoassay
US60/135,417 2000-02-14
US09/503,563 2000-02-14

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP20000932644 EP1179181A4 (en) 1999-05-21 2000-05-19 Microscale diffusion immunoassay
JP2000620357A JP2003500653A (en) 1999-05-21 2000-05-19 Microscale diffusion immunoassay

Publications (3)

Publication Number Publication Date
WO2000072020A1 true WO2000072020A1 (en) 2000-11-30
WO2000072020A8 true true WO2000072020A8 (en) 2001-06-14
WO2000072020A9 true WO2000072020A9 (en) 2002-08-29

Family

ID=26833300

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/013864 WO2000072020A9 (en) 1999-05-21 2000-05-19 Microscale diffusion immunoassay

Country Status (4)

Country Link
US (1) US20020090644A1 (en)
JP (1) JP2003500653A (en)
EP (1) EP1179181A4 (en)
WO (1) WO2000072020A9 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7011791B2 (en) 2000-09-18 2006-03-14 University Of Washington Microfluidic devices for rotational manipulation of the fluidic interface between multiple flow streams
GB0128350D0 (en) * 2001-11-27 2002-01-16 Lab901 Ltd Non-rigid apparatus for microfluidic applications
CA2468674A1 (en) * 2001-12-05 2003-06-12 University Of Washington Microfluidic device and surface decoration process for solid phase affinity binding assays
US20030203504A1 (en) 2002-04-26 2003-10-30 John Hefti Diffusion-based system and method for detecting and monitoring activity of biologic and chemical species
JP2004053417A (en) * 2002-07-19 2004-02-19 National Institute Of Advanced Industrial & Technology Method for analyzing molecules using micro channel
GB0225135D0 (en) * 2002-10-29 2002-12-11 Micro Chemical Systems Ltd Apparatus and method for performing an assay
GB2395196B (en) * 2002-11-14 2006-12-27 Univ Cardiff Microfluidic device and methods for construction and application
US20060076295A1 (en) 2004-03-15 2006-04-13 The Trustees Of Columbia University In The City Of New York Systems and methods of blood-based therapies having a microfluidic membraneless exchange device
CA2518667C (en) * 2003-03-14 2011-07-19 The Trustees Of Columbia University In The City Of New York Systems and methods of blood-based therapies having a microfluidic membraneless exchange device
US7550267B2 (en) * 2004-09-23 2009-06-23 University Of Washington Microscale diffusion immunoassay utilizing multivalent reactants
US7727399B2 (en) 2006-05-22 2010-06-01 The Trustees Of Columbia University In The City Of New York Systems and methods of microfluidic membraneless exchange using filtration of extraction outlet streams
US8496606B2 (en) 2008-02-04 2013-07-30 The Trustees Of Columbia University In The City Of New York Fluid separation devices, systems and methods
GB201216163D0 (en) * 2012-09-11 2012-10-24 Exacsys Ltd Devices and methods for measurement of sample properties
CN105181957B (en) * 2015-07-28 2017-03-15 广东产品质量监督检验研究院 Bisphenol s test kit and method of making and using

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4894146A (en) * 1986-01-27 1990-01-16 University Of Utah Thin channel split flow process and apparatus for particle fractionation
US4849340A (en) * 1987-04-03 1989-07-18 Cardiovascular Diagnostics, Inc. Reaction system element and method for performing prothrombin time assay
US5439578A (en) * 1993-06-03 1995-08-08 The Governors Of The University Of Alberta Multiple capillary biochemical analyzer
DE69628016D1 (en) * 1995-06-16 2003-06-12 Univ Washington Seattle Miniaturized differential extraction device and process
US5948684A (en) * 1997-03-31 1999-09-07 University Of Washington Simultaneous analyte determination and reference balancing in reference T-sensor devices
US5716852A (en) * 1996-03-29 1998-02-10 University Of Washington Microfabricated diffusion-based chemical sensor
EP0910474B1 (en) * 1996-06-14 2004-03-24 University of Washington Absorption-enhanced differential extraction method
US5942443A (en) * 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices

Also Published As

Publication number Publication date Type
JP2003500653U (en) application
US20020090644A1 (en) 2002-07-11 application
WO2000072020A1 (en) 2000-11-30 application
WO2000072020A9 (en) 2002-08-29 application
JP2003500653A (en) 2003-01-07 application
EP1179181A1 (en) 2002-02-13 application
EP1179181A4 (en) 2004-12-29 application

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