WO2000071111A1 - Stimulating transport of glucose into animal tissue by the administration of pinitol - Google Patents

Stimulating transport of glucose into animal tissue by the administration of pinitol Download PDF

Info

Publication number
WO2000071111A1
WO2000071111A1 PCT/US2000/013872 US0013872W WO0071111A1 WO 2000071111 A1 WO2000071111 A1 WO 2000071111A1 US 0013872 W US0013872 W US 0013872W WO 0071111 A1 WO0071111 A1 WO 0071111A1
Authority
WO
WIPO (PCT)
Prior art keywords
pinitol
muscle tissue
compound
insulin
glucose
Prior art date
Application number
PCT/US2000/013872
Other languages
French (fr)
Inventor
Charles E. Weeks
Original Assignee
Humanetics Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Humanetics Corporation filed Critical Humanetics Corporation
Priority to CA002374269A priority Critical patent/CA2374269C/en
Priority to EP00937624A priority patent/EP1183021A4/en
Priority to AU52770/00A priority patent/AU5277000A/en
Priority to US09/857,245 priority patent/US6518318B1/en
Publication of WO2000071111A1 publication Critical patent/WO2000071111A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/075—Ethers or acetals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
    • A61K31/047—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates having two or more hydroxy groups, e.g. sorbitol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics

Definitions

  • the invention relates to the use of pharmaceuticals and dietary supplements to stimulate the transport of glucose into animal tissue, particularly muscle tissue.
  • muscle tissue One of the factors involved in the performance of muscle tissue, especially during such physical activities as prolonged exercise routines and athletic events, is the ability of the muscle tissue to transport sufficient amounts of glucose into the tissue and convert the glucose into useable energy (i.e., ATP). While a wide variety of products and techniques have been developed in an attempt to accelerate the growth and development of muscle tissue (i.e., increasing muscle mass), little activity has focused upon improving the performance of existing muscle tissue.
  • glucose transport and glycogen loading within animal tissue can be stimulated and enhanced by administering an effective amount of a pinitol compound.
  • Such stimulated glucose transport and enhanced glycogen loading is particularly effective for enhancing the performance of muscle tissue.
  • pinitol alone, or in combination with a synergistic amount of insulin is effective for controlling insulin-dependent diabetes.
  • a method of enhancing performance of muscle tissue comprising administering an effective amount of a pinitol compound, preferably pinitol, to a human desiring such enhanced performance
  • a method of increasing glycogen loading in human tissue, including muscle tissue comprising administering an effective amount of a pinitol compound, preferably pinitol, to a human desiring such increased glycogen loading
  • a method of stimulating transport of glucose into human tissue, including muscle tissue comprising administering an effective amount of a pinitol compound, preferably pinitol, to a nondiabetic human desiring such stimulated transport of glucose
  • a method of controlling insulin-dependent diabetes comprising administering an effective amount of a pinitol compound, preferably pinitol, with or without the conjoint administration of an effective amount of
  • the phrase performance of muscle tissue means contraction of the muscle tissue so as effect movement of the body (e.g., running, smiling or throwing a bowling ball) or exertion against a counteracting force (e.g., holding a bowling ball against the force of gravity).
  • the phrase "enhancing performance of muscle tissue” means (i) increasing the force with which the muscle tissue can contract (i.e., strength), (ii) decreasing the time required for the muscle tissue to recover after each contraction or series of contractions (i.e., recovery), and/or (iii) increasing the time period during which the muscle tissue can continuously or repetitively contract (i.e., endurance).
  • the term "pinitol compound” means pinitol, metabolites of pinitol, derivatives of pinitol and mixtures thereof.
  • nondiabetic human means a human who has NOT been diagnosed with non-insulin-dependent type II diabetes.
  • insulin-dependent diabetic means a human who has been diagnosed with insulin-dependent type I diabetes.
  • Pinitol is available from a number of natural sources (such as pine needles, chick peas and soy beans). Pinitol can also be synthetically produced. One procedure for extracting pinitol from soy beans is disclosed in United States Patent No. 5,550,166, which is hereby incorporated by reference. A suitable grade of pinitol is also available from Humanetics Corporation under the mark InzitolTM.
  • pinitol glycosides include pinitol glycosides, pinitol phospholipids, esterified pinitol, lipid-bound pinitol, pinitol phosphates and pinitol phytates.
  • the pinitol compound can be administered by virtually any of the commonly accepted practices for the administration of pharmaceutical preparations and dietary supplements including specifically, but not exclusively, mucosal administration, oral consumption, ocular administration, subcutaneous injection, transdermal administration, etc.
  • Mucosal administration of the active ingredients includes such routes as buccal, endotracheal, nasal, pharyngeal, rectal, sublingual, vaginal, etc.
  • the pinitol compound may be formulated as an emulsion, gum, lozenge, spray, tablet or an inclusion complex such as cyclodextrin inclusion complexes.
  • Nasal administration is conveniently conducted through the use of a sniffing power or nasal spray.
  • the ingredients may be formulated as a cream, douche, enema or suppository.
  • Oral consumption of the active ingredients may be effected by incorporating the ingredients into a food or drink, or formulating the ingredients into a chewable or swallowable tablet or capsule.
  • Ocular administration may be effected by incorporating the active ingredients into a solution or suspension adapted for ocular application such as drops or sprays.
  • Subcutaneous administration involves incorporating the active ingredients into a pharmaceutically acceptable and injectable carrier.
  • the active ingredients may be conveniently incorporated into a lipophilic carrier and formulated as a topical creme or adhesive patch.
  • the range of dosages and dose rates effective for achieving the desired biological response may be determined in accordance with standard industry practices.
  • a dose rate of about 0J to 1,000 mg of pinitol compound per kg body weight per day, preferably about 1 to 100 mg of pinitol compound per kg body weight per day should provide the desired biological response of enhancing the performance of muscle tissue by increasing glycogen loading within the muscle tissue and stimulating the transportation of glucose into the muscle tissue.
  • Such a range of dosages and dose rates are also generally applicable when the pinitol is administered, with or without the conjoint administration of insulin, to control insulin-dependent type I diabetes.
  • Glucose transport was tested and measured in accordance with the protocol set forth in Amira Klip et al., Induction of Sugar Uptake Response to Insulin by Serum Depletion in Fusing L ⁇ Myoblasts, American Journal of Physiology, Vol. 247, No. 3, Part 1 (September 1984), herein incorporated by reference, except that the cell culture was preincubated for only 2 hours and the cells treated with varying concentrations of pinitol, insulin or a combination thereof as set forth in Tables One through Three.
  • the raw data in terms of "counts per minute” as measured with a scintillation counter, is provided in Tables One and Two. while calculated data, in terms of "pmol min "1 mg protein "1 ,” is provided in Table Three.
  • glycogen incorporation is similar to that of J Berger and NS Hayes, Anal Biochem. 261: 159-163 (1998), one of several standard references for radiolabeled (14-C) glucose incorporation into ethanol-precipitable glycogen.
  • Into each well was added 1.25 ml of 1% TritonXlOO. The wells were then placed on a rotating agitator for 30 minutes to dissolve the cells. A 1 ml sample was removed from each well and placed in a 10 ml plastic tube.
  • Into each sample was added 1 ml of a solution containing 18% trichloroacetic acid and 2mg/ml of glycogen (added as carrier).
  • the treated samples were placed on ice for 20 minutes and then centrifuged for 10 minutes at 10,000 RPM with the supernatant transferred to new plastic tubes. The remaining solids were washed by adding 0.3 ml of the trichloracetic acid-glycogen solution to the pellet, and centrifuged again as before. The combined supernatants were added to 4 ml of ice cold ethanol and 0.25 ml of 2% sodium sulphate (to help precipitate glycogen, as in LN. Hung and L.A. Menahan, Biochemical Medicine, Vol. 24: 356-360 (1980)). This was left overnight at 4°C. The precipitate was collected the next day by centrifugation at 10,000 RPM for 10 min.
  • Pinitol can provide a significant stimulation in glycogen formation when administered alone.
  • the conjoint administration of pinitol with low levels of insulin (/ e , 10 "7 M) provides a synergistic enhancement in glycogen formation.

Landscapes

  • Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Obesity (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Endocrinology (AREA)
  • Emergency Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

Enhanced muscle performance, including strength, recovery and endurance, can be achieved by means of improved glucose transport and improved glycogen loading within animal muscle tissue, by administering an effective amount of a pinitol compound. Pinitol alone, or in combination with a synergistic amount of insulin, is effetive for controlling insulin-dependent diabetes.

Description

STIMULATING TRANSPORT OF GLUCOSE
INTO ANIMAL TISSUE
BY THE ADMINISTRATION
OF PINITOL
FIELD OF THE INVENTION
The invention relates to the use of pharmaceuticals and dietary supplements to stimulate the transport of glucose into animal tissue, particularly muscle tissue.
BACKGROUND
One of the factors involved in the performance of muscle tissue, especially during such physical activities as prolonged exercise routines and athletic events, is the ability of the muscle tissue to transport sufficient amounts of glucose into the tissue and convert the glucose into useable energy (i.e., ATP). While a wide variety of products and techniques have been developed in an attempt to accelerate the growth and development of muscle tissue (i.e., increasing muscle mass), little activity has focused upon improving the performance of existing muscle tissue.
SUMMARY OF THE INVENTION
I have discovered that glucose transport and glycogen loading within animal tissue can be stimulated and enhanced by administering an effective amount of a pinitol compound. Such stimulated glucose transport and enhanced glycogen loading is particularly effective for enhancing the performance of muscle tissue.
I have also discovered that pinitol alone, or in combination with a synergistic amount of insulin, is effective for controlling insulin-dependent diabetes. In summary, I have discovered: (i) a method of enhancing performance of muscle tissue comprising administering an effective amount of a pinitol compound, preferably pinitol, to a human desiring such enhanced performance, (ii) a method of increasing glycogen loading in human tissue, including muscle tissue, comprising administering an effective amount of a pinitol compound, preferably pinitol, to a human desiring such increased glycogen loading, (iii) a method of stimulating transport of glucose into human tissue, including muscle tissue, comprising administering an effective amount of a pinitol compound, preferably pinitol, to a nondiabetic human desiring such stimulated transport of glucose, and (v) a method of controlling insulin-dependent diabetes comprising administering an effective amount of a pinitol compound, preferably pinitol, with or without the conjoint administration of an effective amount of insulin, to an insulin-dependent diabetic.
DETAILED DESCRIPTION OF THE INVENTION INCLUDING A BEST MODE
Definitions
As utilized herein, including the claims, the phrase performance of muscle tissue" means contraction of the muscle tissue so as effect movement of the body (e.g., running, smiling or throwing a bowling ball) or exertion against a counteracting force (e.g., holding a bowling ball against the force of gravity).
As utilized herein, including the claims, the phrase "enhancing performance of muscle tissue" means (i) increasing the force with which the muscle tissue can contract (i.e., strength), (ii) decreasing the time required for the muscle tissue to recover after each contraction or series of contractions (i.e., recovery), and/or (iii) increasing the time period during which the muscle tissue can continuously or repetitively contract (i.e., endurance). As utilized herein, including the claims, the term "pinitol compound" means pinitol, metabolites of pinitol, derivatives of pinitol and mixtures thereof.
As utilized herein, including the claims, the term "nondiabetic human" means a human who has NOT been diagnosed with non-insulin-dependent type II diabetes.
As utilized herein, including the claims, the term "insulin-dependent diabetic" means a human who has been diagnosed with insulin-dependent type I diabetes.
Active Ingredient
Pinitol is available from a number of natural sources (such as pine needles, chick peas and soy beans). Pinitol can also be synthetically produced. One procedure for extracting pinitol from soy beans is disclosed in United States Patent No. 5,550,166, which is hereby incorporated by reference. A suitable grade of pinitol is also available from Humanetics Corporation under the mark Inzitol™.
Various derivatives and metabolites of pinitol are also effective for enhancing the performance of muscle tissue by increasing glycogen loading within the muscle tissue and stimulating the transportation of glucose into the muscle tissue. A nonexhaustive listing of suitable derivatives and metabolites include pinitol glycosides, pinitol phospholipids, esterified pinitol, lipid-bound pinitol, pinitol phosphates and pinitol phytates.
Administration
ADMINISTRATION ROUTE
The pinitol compound can be administered by virtually any of the commonly accepted practices for the administration of pharmaceutical preparations and dietary supplements including specifically, but not exclusively, mucosal administration, oral consumption, ocular administration, subcutaneous injection, transdermal administration, etc.
Mucosal administration of the active ingredients includes such routes as buccal, endotracheal, nasal, pharyngeal, rectal, sublingual, vaginal, etc. For administration through the buccal/sublingual/pharyngeal/endotracheal mucosa, the pinitol compound may be formulated as an emulsion, gum, lozenge, spray, tablet or an inclusion complex such as cyclodextrin inclusion complexes. Nasal administration is conveniently conducted through the use of a sniffing power or nasal spray. For rectal and vaginal administration, the ingredients may be formulated as a cream, douche, enema or suppository.
Oral consumption of the active ingredients may be effected by incorporating the ingredients into a food or drink, or formulating the ingredients into a chewable or swallowable tablet or capsule.
Ocular administration may be effected by incorporating the active ingredients into a solution or suspension adapted for ocular application such as drops or sprays.
Subcutaneous administration involves incorporating the active ingredients into a pharmaceutically acceptable and injectable carrier.
For transdermal administration, the active ingredients may be conveniently incorporated into a lipophilic carrier and formulated as a topical creme or adhesive patch.
DOSE RATE
The range of dosages and dose rates effective for achieving the desired biological response may be determined in accordance with standard industry practices. As a general guide, a dose rate of about 0J to 1,000 mg of pinitol compound per kg body weight per day, preferably about 1 to 100 mg of pinitol compound per kg body weight per day, should provide the desired biological response of enhancing the performance of muscle tissue by increasing glycogen loading within the muscle tissue and stimulating the transportation of glucose into the muscle tissue. Such a range of dosages and dose rates are also generally applicable when the pinitol is administered, with or without the conjoint administration of insulin, to control insulin-dependent type I diabetes.
EXPERIMENTAL
Testing Protocol
GLUCOSE UPTAKE
Glucose transport was tested and measured in accordance with the protocol set forth in Amira Klip et al., Induction of Sugar Uptake Response to Insulin by Serum Depletion in Fusing Lβ Myoblasts, American Journal of Physiology, Vol. 247, No. 3, Part 1 (September 1984), herein incorporated by reference, except that the cell culture was preincubated for only 2 hours and the cells treated with varying concentrations of pinitol, insulin or a combination thereof as set forth in Tables One through Three. The raw data, in terms of "counts per minute" as measured with a scintillation counter, is provided in Tables One and Two. while calculated data, in terms of "pmol min"1 mg protein"1 ," is provided in Table Three.
GLYCOGEN FORMATION
The method for glycogen incorporation is similar to that of J Berger and NS Hayes, Anal Biochem. 261: 159-163 (1998), one of several standard references for radiolabeled (14-C) glucose incorporation into ethanol-precipitable glycogen. Into each well was added 1.25 ml of 1% TritonXlOO. The wells were then placed on a rotating agitator for 30 minutes to dissolve the cells. A 1 ml sample was removed from each well and placed in a 10 ml plastic tube. Into each sample was added 1 ml of a solution containing 18% trichloroacetic acid and 2mg/ml of glycogen (added as carrier). The treated samples were placed on ice for 20 minutes and then centrifuged for 10 minutes at 10,000 RPM with the supernatant transferred to new plastic tubes. The remaining solids were washed by adding 0.3 ml of the trichloracetic acid-glycogen solution to the pellet, and centrifuged again as before. The combined supernatants were added to 4 ml of ice cold ethanol and 0.25 ml of 2% sodium sulphate (to help precipitate glycogen, as in LN. Hung and L.A. Menahan, Biochemical Medicine, Vol. 24: 356-360 (1980)). This was left overnight at 4°C. The precipitate was collected the next day by centrifugation at 10,000 RPM for 10 min. The precipitate was redisolved in water (2 ml) and reprecipitated as before with ethanol and sodium sulphate. The precipitate was dissolved in water, added to scintillation fluid, and radioactivity determined. Results are reported in Tables Four and Five.
Experiment One GLUCOSE UPTAKE (Concentration)
TABLE ONE
Figure imgf000007_0001
CONCLUSION: Pinitol alone does not meaningfully stimulate glucose transport at low concentrations (e.g., 0J4 mM), but can provide a significant stimulation in glucose transport at higher concentrations (e.g., 1.44 mM). Experiment Two
GLUCOSE UPTAKE
(Comparison of Pinitol, Insulin and Combination of Pinitol and Insulin)
TABLETWO
Figure imgf000008_0001
TABLE THREE
Figure imgf000008_0002
CONCLUSION: Both pinitol and insulin, at higher concentrations, can provide a significant stimulation in glucose transport when administered alone, with the conjoint administration of pinitol and insulin, even at lower concentrations, providing a synergistic enhancement in glucose transport. Experiment Three
GL YCOGEN FORMA TION
(Comparison of Pinitol, Insulin and Combination of Pinitol and Insulin)
TABLE FOUR
Figure imgf000009_0001
TABLE FIVE
Figure imgf000010_0001
CONCLUSION: Pinitol can provide a significant stimulation in glycogen formation when administered alone. The conjoint administration of pinitol with low levels of insulin (/ e , 10"7 M) provides a synergistic enhancement in glycogen formation.

Claims

I Claim:
1. A method of enhancing performance of muscle tissue comprising administering an effective amount of a pinitol compound to a human desiring such enhanced performance.
2. The method of claim 1 wherein enhanced performance of muscle tissue is an increase in the strength of the muscle tissue.
3. The method of claim 1 wherein enhanced performance of muscle tissue is a decrease in recovery time of the muscle tissue.
4. The method of claim 1 wherein enhanced performance of muscle tissue is an increase in the endurance of the muscle tissue.
5. The method of claim 1 wherein the pinitol compound is pinitol.
6. A method of increasing glycogen loading in human tissue comprising administering an effective amount of a pinitol compound to a human desiring such increased glycogen loading.
7. The method of claim 6 wherein the pinitol compound is pinitol.
8. The method of claim 6 wherein the human tissue is muscle tissue.
9. A method of stimulating transport of glucose into human tissue comprising administering an effective amount of a pinitol compound to a nondiabetic human desiring such stimulated transport of glucose.
10. The method of claim 9 wherein the pinitol compound is pinitol.
11. The method of claim 9 wherein the human tissue is muscle tissue.
12. A method of controlling insulin-dependent diabetes comprising administering an effective amount of a pinitol compound to an insulin-dependent diabetic.
13. The method of claim 12 further comprising the conjoint administration of an effective amount of insulin to the insulin-dependent diabetic.
14. The method of claim 12 wherein the pinitol compound is pinitol.
PCT/US2000/013872 1999-05-20 2000-05-19 Stimulating transport of glucose into animal tissue by the administration of pinitol WO2000071111A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002374269A CA2374269C (en) 1999-05-20 2000-05-19 Stimulating transport of glucose into animal tissue by the administration of pinitol
EP00937624A EP1183021A4 (en) 1999-05-20 2000-05-19 Stimulating transport of glucose into animal tissue by the administration of pinitol
AU52770/00A AU5277000A (en) 1999-05-20 2000-05-19 Stimulating transport of glucose into animal tissue by the administration of pinitol
US09/857,245 US6518318B1 (en) 1999-05-20 2000-05-19 Stimulating transport of glucose into animal tissue by the administration of pinitol

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13500899P 1999-05-20 1999-05-20
US60/135,008 1999-05-20

Publications (1)

Publication Number Publication Date
WO2000071111A1 true WO2000071111A1 (en) 2000-11-30

Family

ID=22466083

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/013872 WO2000071111A1 (en) 1999-05-20 2000-05-19 Stimulating transport of glucose into animal tissue by the administration of pinitol

Country Status (5)

Country Link
US (1) US6518318B1 (en)
EP (1) EP1183021A4 (en)
AU (1) AU5277000A (en)
CA (1) CA2374269C (en)
WO (1) WO2000071111A1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011123572A1 (en) 2010-03-31 2011-10-06 The Scripps Research Institute Reprogramming cells
WO2016150573A1 (en) * 2015-03-26 2016-09-29 Capri Sun Ag Compositions for use in food products

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060024367A1 (en) * 1998-05-28 2006-02-02 Byrd Edward A Controlled release alpha lipoic acid formulation with an inositol compound
US20030212134A1 (en) * 2001-04-16 2003-11-13 Dykstra John C. Combination of pinitol and creatine to enhance uptake and retention of creatine
US8193250B2 (en) * 2004-10-22 2012-06-05 Mount Sinai School Of Medicine Compositions and methods for treating alzheimer's disease and related disorders and promoting a healthy nervous system
US20100119499A1 (en) * 2009-09-17 2010-05-13 Kneller Bruce W Stilbene-based compositions and methods of use therefor
US8999424B2 (en) 2010-10-26 2015-04-07 Advanced Bio Development, Inc. Performance enhancing composition and method of delivering nutrients
US9522161B2 (en) 2010-10-26 2016-12-20 Advanced Bio Development, Inc. Performance enhancing composition and method of delivering nutrients

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5550166A (en) * 1995-03-17 1996-08-27 Ostlund; Richard E. Pinitol and derivatives thereof for the treatment of metabolic disorders

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4446064A (en) 1980-12-19 1984-05-01 The University Of Virginia Alumni Patents Foundation Insulin mediator substance
US4952396A (en) 1986-11-19 1990-08-28 Linus Pauling Institute Of Science & Medicine Method of using phytic acid for inhibiting tumor growth
US5428066A (en) 1989-03-08 1995-06-27 Larner; Joseph Method of reducing elevated blood sugar in humans
US5427956A (en) 1989-03-08 1995-06-27 The University Of Virginia Patent Foundation Quantitative analysis for diabetic condition predictor
US5122603A (en) 1989-03-08 1992-06-16 The University Of Virginia Alumni Patents Foundation Purified insulin mediators and purification process for same
US5183764A (en) 1989-03-08 1993-02-02 University Of Virginia Alumni Patents Foundation Quantitative analysis for diabetic condition predictor involving chiro-inositol
JP2834321B2 (en) 1989-03-08 1998-12-09 ザ ユニヴァーシティー オブ ヴァージニア アルムニ パテンツ ファウンデーション Diabetes screening method
WO1990010439A1 (en) 1989-03-08 1990-09-20 The University Of Virginia Alumni Patents Foundation Dietary supplement for insulin-resistant diabetics
ATE151464T1 (en) 1990-02-08 1997-04-15 Univ Virginia QUANTITATIVE ANALYSIS OF CHIROINOSITOL AS AN INDICATOR OF DIABETIC CONDITION
SE467340B (en) 1990-07-04 1992-07-06 Perstorp Ab USE OF INOSITOL MONOPHOSPHATE FOR THE PREPARATION OF AN EFFECTIVE EFFECT AS A NEUROPEPTID Y (NPY) ANTAGONIST
US5112814A (en) 1990-10-24 1992-05-12 Robert Sabin Method of treatment of Parkinson's disease using phytic acid
US5091596A (en) 1990-12-20 1992-02-25 Univ. Of Va. Alumni Patents Foundation Method for producing chiro-inositol
AU662720B2 (en) 1991-01-23 1995-09-14 University Of Virginia Patent Foundation Method of reducing elevated blood sugar levels in humans
US5256697A (en) 1992-04-16 1993-10-26 Abbott Laboratories Method of administering pyruvate and methods of synthesizing pyruvate precursors
US5834473A (en) 1993-04-29 1998-11-10 Cultor, Ltd. Method for treating coccidiosis
US5516950A (en) 1994-04-15 1996-05-14 Insmed Pharmaceuticals, Inc. Method for the production of D-chiroinositol
US5652221A (en) 1994-11-07 1997-07-29 The University Of Virginia Patent Foundation Method of treating defective glucose metabolism using synthetic insulin substances
US5906979A (en) 1998-01-27 1999-05-25 Insmed Pharmaceuticals, Inc. Compositions and methods for treating metabolic diseases characterized by hyperandrogenism and/or anovulation and/or infertility
US20010039297A1 (en) 1998-05-19 2001-11-08 Insmed Pharmaceuticals, Inc. Compositions and method for improving insulin sensivity and glucose metabolism in individuals with impaired fasting glucose or impaired glucose tolerance
WO1999060406A1 (en) 1998-05-21 1999-11-25 Insmed Pharmaceuticals, Inc. Myo-inositol as a predictor of glycemic control in mammals
JP2002542295A (en) 1999-04-27 2002-12-10 インスメッド ファーマシューティカルズ， インコーポレイテッド Compositions and methods for improving insulin sensitivity and glucose metabolism in mammals
AU2001257422A1 (en) 2000-04-28 2001-11-12 Insmed Pharmaceuticals, Inc. Use of d-chiro-inositol in the treatment of conditions associated with hypothalamic gene expression
US6645485B2 (en) 2000-05-10 2003-11-11 Allan R. Dunn Method of treating inflammation in the joints of a body
US6277396B1 (en) 2000-05-11 2001-08-21 Maximum Human Performance, Inc. Dietary supplement containing a thermogenic substance and an adrenal support substance
US6759390B2 (en) 2000-05-12 2004-07-06 Manuel Martin-Lomas Compounds and their uses

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5550166A (en) * 1995-03-17 1996-08-27 Ostlund; Richard E. Pinitol and derivatives thereof for the treatment of metabolic disorders
US5827896A (en) * 1995-03-17 1998-10-27 Washington University Pinitol and derivatives thereof for the treatment of metabolic disorders

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1183021A4 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011123572A1 (en) 2010-03-31 2011-10-06 The Scripps Research Institute Reprogramming cells
EP3199623A1 (en) 2010-03-31 2017-08-02 The Scripps Research Institute Reprogramming cells
EP3936608A1 (en) 2010-03-31 2022-01-12 The Scripps Research Institute Reprogramming cells
WO2016150573A1 (en) * 2015-03-26 2016-09-29 Capri Sun Ag Compositions for use in food products

Also Published As

Publication number Publication date
EP1183021A1 (en) 2002-03-06
CA2374269A1 (en) 2000-11-30
CA2374269C (en) 2007-04-24
EP1183021A4 (en) 2004-09-15
AU5277000A (en) 2000-12-12
US6518318B1 (en) 2003-02-11

Similar Documents

Publication Publication Date Title
Sattler et al. Food-induced histaminosis as an epidemiological problem: plasma histamine elevation and haemodynamic alterations after oral histamine administration and blockade of diamine oxidase (DAO)
Knochel Rhabdomyolysis and myglobinuria
EP0282206B1 (en) Arylcyclobutylalkylamine-derivative for the treatment of parkinson's disease
Schiller et al. Studies of the mechanism of the antidiarrheal effect of codeine
Willson et al. Alkalosis and low plasma potassium in a case of Cushing's syndrome: a metabolic study
Reid et al. Sodium γ-resorcylate in rheumatic fever
Papp et al. Toxic effects of iproniazid in a patient with angina
EP0697862A4 (en) Pharmaceutical compositions and methods using isobutyramide for treating betaglobin disorders
WO2023005265A1 (en) Application of nucleotide mixture in preparation of formulations for preventing or alleviating senile sarcopaenia
US6518318B1 (en) Stimulating transport of glucose into animal tissue by the administration of pinitol
Brodie et al. Observations on G-25671, A Phenylbutazone Analogue (4-(phenylthioethyl)− 1, 2-diphenyl 3, 5-pyrazolidinedione).
Barach et al. Inhalation of Nebulized Promin in Experimental Tuberculosis: Sodium P, P'-Diaminodiphenylsulfone-N, N'-Didextrose Sulfonate
Bédard et al. Biphasic effect of estradiol and domperidone on lingual dyskinesia in monkeys
Elton et al. Effect of moderate obesity on glucose transport in human muscle
KR20040084168A (en) Use of pinitol or chiro-inositol for preventing or treating liver diseases
Fung et al. The importance of calcium in amphetamine-induced turning behavior in mice with unilateral nigro-striatal lesions
Yokozawa et al. Variations in the distribution of methylguanidine with the progression of renal failure after methylguanidine loading
Ibbertson et al. Rapidly acting thyroid hormones and their cardiac action
AU651824B2 (en) Pharmaceutical composition
WO1994005273A1 (en) Gastrointestinal compositions containing dimethylsulfone and dimethylsulfoxide
EP0106812A2 (en) Pharmaceutical composition containing a fibrinolytic agent and a diffusion factor, useful for the treatment of the myocardium infarction
US6197818B1 (en) Drug for treating diabetic nephrosis
MacDonald et al. Studies of bile lipids and bile acids of wild North American black bears in Nova Scotia, showing a high content of ursodeoxycholic acid
WO2004048358A1 (en) Control of cancer with annonaceous extracts
Rotmensch et al. Furosemide-induced forced diuresis in digoxin intoxication

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AU CA JP US

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 09857245

Country of ref document: US

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
ENP Entry into the national phase

Ref document number: 2374269

Country of ref document: CA

Ref country code: CA

Ref document number: 2374269

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 2000937624

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2000937624

Country of ref document: EP

NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Ref document number: 2000937624

Country of ref document: EP