WO2000053320A1 - Pumping device for transferring at least a fluid into a consumable - Google Patents

Pumping device for transferring at least a fluid into a consumable Download PDF

Info

Publication number
WO2000053320A1
WO2000053320A1 PCT/FR2000/000580 FR0000580W WO0053320A1 WO 2000053320 A1 WO2000053320 A1 WO 2000053320A1 FR 0000580 W FR0000580 W FR 0000580W WO 0053320 A1 WO0053320 A1 WO 0053320A1
Authority
WO
WIPO (PCT)
Prior art keywords
compartment
compartments
characterized
transfer
liquid
Prior art date
Application number
PCT/FR2000/000580
Other languages
French (fr)
Inventor
Bruno Colin
Original Assignee
Biomerieux S.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR99/03031 priority Critical
Priority to FR9903031A priority patent/FR2790681B1/en
Application filed by Biomerieux S.A. filed Critical Biomerieux S.A.
Publication of WO2000053320A1 publication Critical patent/WO2000053320A1/en

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0816Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0481Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure squeezing of channels or chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/06Valves, specific forms thereof
    • B01L2400/0633Valves, specific forms thereof with moving parts

Abstract

The invention concerns a pumping device (1) for transferring a fluid sample (2) into a sealed consumable (3), the transfer being performed between at least a first compartment, said to be initial, (4) and at least a second compartment, said to be, receiving, (5) via a narrowed section such as a flow path (6). The invention is characterised in that the point of intersection between an initial compartment (4) and the narrowed section is located in the lower part, and preferably at the lowest level, of said initial compartment (4), that the point of intersection between the receiving compartment (5) and said narrowed part is located in the upper part, and preferably at the highest level, of said receiving compartment (5), and at least one of the initial (4) and/or receiving (5) compartment can be deformed, such that each deformation activates the transfer of all or part of the sample (2), The invention is particularly applicable to microfluidic devices used in biology.

Description

pumping means for transferring at least one fluid in a consumable

DESCRIPTION

The present invention relates to a pumping apparatus to allow transfer of a fluid sample in a sealed consumable, the transfer being performed between at least one first compartment, said starting, and at least one second compartment, said check, via a constriction such as a channel. The invention also relates to a method for implementing such a device.

The state of the art consists of EP-A-0,381,501 which proposes an apparatus for performing the amplification of nucleic acids using polymerase chain reaction technology (PCR), while preventing the salting out of the nucleic acids in the atmosphere. To do this, this unit is sealed and the transfer of biological liquids contained therein, between the compartments is effected by external action exerted on a flexible wall of a compartment. To do so, it uses a roller that compresses once the starting compartment in order to drive the liquid to a destination compartment.

WO-A-97/27324 shows substantially the same configuration as the transfer liquid in a sealed compartment is formed by the external action exerted on a flexible wall of a compartment. The aim is also the same as it allows nucleic acid amplification using PCR technology, without contamination, the device is sealed. It comprises a pumping device to allow the transfer of a fluid sample in a sealed consumable, the transfer being performed between at least one first compartment, said starting, and at least one second compartment, said check, via a such as a channel narrowing. Moreover, it adds that at least one of the starting compartments and / or destination may be deformed, so that each deformation actuates the transfer of all or part of the sample.

Nevertheless, the position of departure and arrival compartments is not important as the invention of the applicants, only the position of the intersection points is essential, specifically, "the point of intersection between a start compartment and shrinkage is positioned in the lower part, and preferably at the lowest level, this starting compartment, and the point of intersection between an inlet compartment and said constriction is positioned in the upper part, and preferably at most about up of the inlet chamber. It is these two features that allow the complete transfer of the liquid in the starting compartment to the inlet compartment playing on gravity. Such is not the case of the document cited above as to the supply chambers which contain and which contain either the structural elements to perform amplification, the polymerase enzyme is, this point of intersection is not in the lowest position. Besides the map of the international application is not vertically but horizontally, as specified an upper surface and a lower surface connected to each other by an edge. This document of the prior art provides that a compartment can be deformed, so that each deformation actuates the transfer of all or part of the sample, this deformation is not due to the deformation of the film but only it is due to the presence of an attached cushion on the film, which does not facilitate the manufacture of such a system.

According to EP-A-0705978, the problem addressed to the accuracy of the pumped volume. The solution provided is therefore to limit the movement of this membrane precisely by the shape of the pump structure. This structure of the pump is constituted by a diaphragm which separates a cavity into two concave parts, the upper (fluid) a the other lower (air or vacuum). The liquid can not enter or leave the concave upper part by a single channel, referenced 41, which is in the upper position.

This structure is completely different from that according to the invention of the applicant, and the problem-solution approach is also different, since our invention aims to optimize the transfer of a liquid avoiding bubbles.

All these devices have a major drawback regarding the transfer efficiency. Thus, they do not reflect the position of the compartments between them and compartments with respect to transfer channels. It is therefore possible that the presence of at least one air bubble interfere or prevent the smooth transfer of liquid from a starting chamber to an arrival chamber. This can lead to disturbances in the amplification and skew the results of the analysis.

The present invention proposes to solve this problem by positioning advantageously on the one hand, the starting or compartments with respect to the arrival or compartments, and secondly, the compartments relative to the transfer channels.

To this end, the present invention relates to a pumping apparatus to allow transfer of a fluid sample in a sealed consumable, the transfer being performed between at least one first compartment, said starting, and at least one second compartment, said arrival, via a constriction such as a channel, characterized in that the point of intersection between a starting chamber and the constriction is positioned in the lower part, and preferably at the lowest level, this starting compartment that the point of intersection between an inlet compartment and said constriction is positioned in the upper part, and preferably at the highest level, of the inlet chamber and at least one of the starting compartments and / or vent can be deformed, so that each deformation activates the transfer of the sample. This narrowing may be made in addition by a channel, a pipe of greater or lesser size, either in length, diameter or cross-section, it can also be a single bottleneck. The only requirement is that, at the point of intersection of this constriction with the starting compartment or arrival, the narrowing section is less than the section of the compartment.

According to a preferred embodiment, the intersection point between a starting chamber and the constriction is in contact with the sample, and the point of intersection between an inlet compartment and said shrinkage is not in contact with said sample. According to an embodiment variant, the starting compartments are placed vertically substantially above or arrival compartments. The term essentially be interpreted to mean that the two compartments are positioned one above the other relative to the vertical. The notions of verticality and horizontality are always given in this application in relation to the force of gravity. However it is also conceivable that we can overcome this force, for example by performing a centrifugation in which case, these notions are then related to the centrifugal force experienced by the consumable sealed, the verticality being constituted by a plane containing the resultant of the centrifugal force and the horizontal being comprised of a plane perpendicular to this resultant. Other systems allow to overcome this force as the action of a magnetic field with transport systems ferrofluids, the action of an electric field as described for the electrical and hydrodynamic pumps (Richter et al. , Sensors and Actuators, 29, pi 59-165, 1991). As centrifugation, the notion of verticality and horizontality is defined in relation to the resultant of the force which causes the displacement. In this case the force aims to position the liquid at the intersection between the starting compartment and shrinking.

According to a preferred embodiment, the intersection point between a starting chamber and the constriction is in the upper position relative to the point of intersection between an inlet compartment and said shrinkage. This upper position is to be interpreted as being in a first horizontal plane containing the first intersection point mentioned above, in the upper position relative to a second horizontal plane containing the first point mentioned above intersection.

Preferably, each compartment can be deformed has at least one partition which can be deformed, such as a flexible film bonded to at least one side of the consumable.

The supply is a card that operates in an inclined or vertical position, preferably vertically. In the case of the force of gravity described above, the concept of an inclined position is measured with respect to the angle between the card with a horizontal plane. This angle must be greater than 10 °, preferably greater than 45 ° and preferably 90 ° which corresponds to the vertical position. According to a variant embodiment, each constriction is longitudinally driven, in whole or part, by at least one tab which facilitates drainage of the fluid sample.

According to another embodiment, at least one of the compartments is associated with a buffer volume.

Such a buffer volume is well described and protected in the patent application filed by the Applicant on the same day as the present invention and entitled "fill Analysis Chart improved". The content of the description of this patent application is considered incorporated in the present invention.

The present invention also relates to a pumping method which comprises deforming at least once a device as described above.

In a first variant, the method consists in deforming at least one of the starting compartments to create a higher than normal pressure in the gas volume of this compartment, to transfer all or part of the liquid contained by said liquid in at least one compartment arrival.

In a second variant, the method consists in deforming at least one of arrival compartments to create an overpressure in the gas volume of this compartment, to transfer all or part of the gas contained by said liquid in at least a start compartment.

According to a third variant, the process is mixing between the two previous techniques. He alternately includes:

- deforming at least one of the starting compartments to create a higher than normal pressure in the gas volume of this compartment, to transfer all or part of the liquid contained by said liquid in at least one inlet chamber, and

- deforming at least one of arrival compartments to create a higher than normal pressure in the gas volume of this compartment, to transfer all or part of the gas contained by said liquid in at least a start compartment.

According to a fourth variant, in the event that a buffer volume is associated with at least one of the starting compartments and / or arrival, the method consists in simultaneously deform the starting compartment and / or arrival and buffer volume. Thus in the case of an analysis card whose cavities are covered by a flexible film, when pressed on the flexible film to compress the volume of one of the compartments, it is also possible to simultaneously compress the buffer volume associated with it. It is therefore particularly interesting to have a compartment and a buffer volume which are located on two opposite sides of the card, at the same level with each other, so the two pressures on each of the two containers are added . Of course, it is necessary that each container is covered with a flexible film, which may be the same, provided that said film take sandwich said map. It is of course possible to combine the fourth embodiment with the other embodiments.

Various means exist for compressing the compartment for example, a movable firing pin between two positions carrying a series of successive pressure on the compartment. The striker may be movable for example by the action of an electric or pneumatic motor. This hammer can be made of any material such as a metal, a plastic, provided that its mechanical strength enables it to exert a pressure or repeated presses of the compartment which can be deformed.

The use of such a device relates to a consumable for the analysis of one or more different liquid samples, in which one seeks to identify one or more analytes according to any simple or complex analysis process involving one or more various reagents depending on the chemical, physical or biological nature of the analytes. The technical principles defined below are not limited to a particular analyte, the only requirement being that the analyte is distributed in the sample to be analyzed in suspension or in solution. In particular, the analysis process implemented can be done, as homogeneous or heterogeneous or mixed.

A particular embodiment, such a non-limiting consumable relates to biological analysis of one or more ligands, requiring for their detection and / or quantification using one or more anti-ligands. Ligand by any biological species is meant for example, an antigen, antigen fragment, a peptide, an antibody, an antibody fragment, a hapten, a nucleic acid, a nucleic acid fragment, a hormone, vitamin. An example of application of analytical techniques concerns immunoassays, whatever their format, by direct analysis or competition. Another example of application relates to the detection and / or quantification of nucleic acids comprising all the operations necessary for this detection and / or quantification this from any sample containing the target nucleic acids. Among these operations may be mentioned lysis, fluidization, concentration, the enzymatic amplification of nucleic acids steps, the steps of detecting incorporating a hybridization step for example using a DNA chip or a labeled probe. The patent application WO-A-

97/02357 explicit various steps required in the case of nucleic acid analysis.

The concept of sealed supply is particularly important if an enzymatic amplification reaction is performed in the consumable, since contamination problems associated with these reactions can be avoided by using a consumable and is sealed particularly advantageous to have a simple system for moving liquids as described herein. The concept of sealed consumable should be understood as a consumable sealed during certain phases of the process and in particular during the fluid displacement phase by pumping. Indeed, it is necessary to introduce a sample containing one or more ligands to be analyzed in a consumable to perform the analysis of said ligands.

At this stage of the process the supply must be opened. Similarly, it is not necessary that all of the consumable is sealed to implement the invention. The fluidic part concerned with the pumping device may be isolated for example by a valve system while another portion is open outwardly to bring the reactants at a predetermined location of the consumable for further reactions.

The accompanying figures are given by way of explanatory example and have no limiting character. They will better understand the invention. 1 shows a partial longitudinal sectional view of a pumping device of a consumable sealed according to the present invention, in the embodiment as simple as possible, since there is a departure compartment and a compartment 'arrival. 2 shows a partial cross-sectional view of a pumping device according to Figure 1 compartment

3 shows a partial longitudinal section of a pumping device of a consumable sealed according to the present invention, in one embodiment more complex, since there is a compartment and from three arrival compartments .

4 shows a longitudinal sectional view of a sealed consumable, which comprises a pumping device according to the present invention, in one embodiment the complex substantially identical to Figure 2, but with three significant differences. First, there is a starting compartment and five compartments arrival. Then the arrival of compartments have a particular configuration. And implantation of the starting compartment from arrival compartments is different from the previous figures.

5 shows a partial longitudinal section of a pumping device of a consumable sealed according to the present invention, in one embodiment more complex, since there are three compartments and three from arrival compartments .

Finally, Figure 6 shows a partial cross section similar to Figure 2 but wherein the flexible film of the pumping device undergoes an external force that induces pumping.

The present invention relates to a pumping device 1 property shown in Figures 1 to 5, which is particularly well adapted to transfer fluids, whether in liquid or gaseous form, inside a consumable sealed 3.

It should first be noted that Figures 1 to 3 and 5 are longitudinal sectional views along different pumping device of the embodiments

1 but scratches that would normally be present on the periphery of said device 1, were removed to facilitate the understanding of the mechanism. It is therefore obvious that must be understood that the elements shown, namely from compartments 4, arrival of the compartments 5, strictures or channels 6, are actually embedded in a consumable 3 as is well shown in Figure 4.

1 shows a simple embodiment of the present invention. It is characterized by the presence of a first from said compartment 4 located at the top and a second compartment said arrival 5 located in lower position. Of course, the shape of the compartments 4 and 5 and their respective volumes may be different from each other but also with respect to the representations in FIGS. The two compartments 4 and 5 are connected to each other by a channel 6 whose shape is adapted. Similarly, the volume of the channel 6 must be of a volume appropriate for the size of the compartments and the positioning of the compartments between them. Details will be made thereafter.

It is noted that the channel implantation point 6 with respect to the compartment meets certain characteristics. Thus, this insertion point is located at the lowest of the starting compartment 4 when it is located at the top of the inlet chamber 5. As will be easily noticed, a fluidic sample 2 is present in the pumping device 1. This sample 2 is in fact liquid. The point of intersection between the channel 6 and from compartment 4 is therefore in contact with the sample 2 which, under the action of gravity, leaving the bottom of said compartment 4. Conversely the point intersection of the channel 6 with the compartment 5 is in contact with the air contained in said compartment 5, the liquid 2 is present throughout the pipeline 6. the fluid sample 2 is present only in the bottom compartment 5. as shown in Figure 1, so there is an alternation between the gas consisting of air and the sample consisting of a liquid 2.

The consumable sealed 3 containing the pumping device 1 is partially shown in Figure 2, which shows a sectional view along AA of Figure 1. It is noted that it is substantially of a board having cavities, said cavities being bounded on one side by the material of the consumable 3, for example plastic, and on the other side by a thin wall 7, which has a flexibility, it may be a film polyethylene or any other material which can be distorted, such as silicone, latex, a polyimide. The nature of the flexible film may also vary depending on the nature of the analysis card and tested fluids, in particular for reasons of compatibility. For example, a polymer film TPX (polyméthylepentène) or BOPP (bi-oriented polypropylene) allows for biological testing. The attachment of these films can be accomplished by gluing (glue coating, such as silicone adhesives on the film) or by welding. An example of BOPP adhesive is supplied by the company

BioMérieux Inc. (St. Louis, MO, USA) under the reference 022004-2184.

In terms of implementation, the test card is obtained by machining a technical plastic such as high impact polystyrene R540E reference Goodfellow, compatible with the treated liquid. In an industrial embodiment, the card could be obtained by precision molding, but other manufacturing methods, and particularly those used in semiconductor techniques such as those described in patent application WO-A-97/02357 are suitable for the manufacture of the test card.

It is therefore easy to understand the functioning of the internal pump to the consumable sealed 3 which operates simply by external pressure F on the flexible film 7, as is well shown in Figure 6. When said film 7 is compressed the volume of air the starting compartment 4 will therefore decrease, the liquid 2 is itself incompressible, it will then pass along the channel 6 and come to flow into the inlet chamber 5. of course, in order to initiate such a device pump 1, it is necessary that the flexible film 7 is the displacement of a volume sufficient to allow the transfer of a quantity of liquid sample 2 which is greater than the total volume of the channel 6.

Another condition to operate the device is to have an offset of the liquid when the liquid is located at the intersection of channels

6 or 8 and the check-in compartment 5. The liquid falling into the compartment 5 can not rise into the channels 6 or 8 upon release of the pressure on the portion which can be deformed.

By several successive actions on said film 7, it is possible to transfer all of the fluid sample 2 from the start of compartment 4 to the inlet compartment 5.

It is also possible to imagine that this system is also found at the arrival compartment 5 only and that the pumping is then performed by pressing a film 7 present only at that finish compartment 5. In this case, the starting volume of the inlet compartment, once compressed, is decreased and the air is transferred via the channel 6 into the starting compartment 4. in the same manner as above, it is necessary that the volume of air expelled Check the compartment 5 is large enough to reach the starting compartment 4.

To do so, the volume of displaced air should be greater than the volume of the channel 6.

According to a final alternative, it is possible to press alternately on the film 7 covering from the compartment 4 and the film 7 covering the inlet chamber 5, in order to accelerate the transfer movement of the pumping device 1.

According to Figure 3, another embodiment is shown comprising a single from compartment 4 and three check-compartments 5. To do so, the channel 6 has a particular structure, since there is a primary channel 8 for each compartment 4 or 5 and an intermediate channel 9 to make the connection between the set of primary channels 8. Although this is not shown in the figures, the shape of the various channels, such as channels 6, 8, 9, 10 and 11 must have a shape adapted to the different embodiments, in particular when a distribution function exists in the system with a multitude of arrival compartments, those skilled in the art will choose a shape for each channel allowing a distribution balanced including playing on the losses. Elbows, constrictions are, for example, means for modulating this loss and not to favor the preferential filling a compartment of arrival. On the contrary, if the volumes transferred to the arrival compartments must be different depending on the reaction conditions to occur in these compartments, the skilled person can play on these channels forms to facilitate filling.

The size and nature of the channel will also be chosen to allow the gas-liquid exchange required to operate the pumping device. For example, in the case of transferring a liquid according to Figure 1, the section of the constriction of channel 6 will be adjusted according to the viscosity of the liquid to allow the air bubble to rise. It is particularly advantageous to minimize the volume of shrinkage channel 6 relative to the volume of liquid to be transferred to avoid dead volumes especially if the starting compartment is not located above, over the notion of gravity , or the arrival of compartments.

A system of suitably arranged valves also control the filling of arrival compartments optionally in combination with the shape of the channels. These valves can have different functions such as a distribution function are used to guide the fluid in a particular direction, to change the distribution channel 11 of Figure 4 or a closing function / opening to isolate the map or compartment as on the valve 15 of Figure 4.

Figure 5 is a particular embodiment wherein there are three from compartments 4 and three check-compartments 5. The channel or narrowing 6 is then in a slightly more complex structure since there is for each compartment 4 or 5 a primary channel 8, as already shown in Figure 3, but also an intermediate channel 9 for each group from compartments 4 or end 5. Preferably, the two intermediate channels 9 are positioned parallel to the one to the other. Between these two channels 9 are present both called secondary channel 10. The number of secondary channels 10 is absolutely not taxed. There may be one or more. On the embodiment of Figure 5, two channels 10 are actually arranged to allow a better distribution of liquid samples 2, which are transferred from the starting compartments 4 to the arrival compartments 5. This type of reactions requires also well quantify the volumes transferred. Or the present invention provides such a controlled distribution. This function will be explained later.

To another embodiment not shown, it is also possible to consider transferring the gas and the liquid used as an insulator between the various gas samples.

The present invention must therefore be understood as a means for reversing the role of gas and liquid since each compartment, at a time of the pumping process is partially filled with one and the other component. Incidentally, it should be noted that at the beginning of pumping, the starting compartment can be filled with fluid and full of gas supply compartment. Similarly, it is also possible that said arrival compartment already contains at least one substance, which may be liquid and / or solid. It may for example be a coating containing at least one reactant for reacting with the sample transferred 2.

Figure 4 shows an embodiment substantially closer industrial models can be manufactured. This is a substantially identical system in Figure 3 since there is a single starting compartment 4 and 5 compartments five finish.

There are also substantial differences between these Figures 3 and 4. Thus in Figure 4, you immediately notice that the starting compartment 4 is located below the inlet compartment 5. In fact, there are no limitations positioning the different compartments 4 and 5 to each other, and preferably however, it is easier to use such a device when the liquid column 2 present in the starting compartment 4 facilitates the transfer by gravity towards the compartments 5. the embodiments of figures 1 to 3 and 5 are particularly interesting. A second difference lies in the structure of arrival compartments. These comprise upper position 13 an apparatus for breaking the bubbles which also is the subject of a patent application filing of the Applicant as indicated above. Also as part of this other patent application, we note the presence in the upper position of the apparatus 13, an orifice 14 which allows communication between said buffer volume 13 and a device whose function is explained in the other patent application, buffer volume that is present on the other face of the consumable 3 having a form of map. Another difference lies in the presence of an input channel 12 for injecting or transferring the fluid sample 2 from another consumable or from another part of the consumable 3 embodiment which is not shown in this figure, the sample 2 being so transferred to a distribution channel 11 located substantially at the center of the card. Since this channel 11, a number of primary channel 8 connects to channel 11 all compartments 4 and 5. Thus, it is possible to have a liquid 17 within the compartment 4 from this liquid 17 being inert with regard to the liquid sample 2 which has been introduced. This liquid 17 is used to allow for actuating and pushing the sample 2 present in the dispensing channel 11 to the arrival compartments 5 via the primary channels 8. It is also noted in this figure 4, the presence of a outlet channel 16 of the consumable 3. This output 16 enables the output of a sample 2 contained in at least one of arrival compartments 5. the choice of the compartment or compartments 5 finish that will be drained is effected by via valves 15 located at all of the outlet pipe 16. it is also noted that there is a valve 15 at the inlet channel 12.

The total volume of liquid transferred by that device can vary from 0.5 to 5000 microliters, preferably from 2 to 2000 microliters, and preferably from 5 to 1000 microlitres. In the case of large volume such as greater than 500 microlitres, preferably chosen one embodiment by successive pressure to transfer liquid volume fractions between 5 and 100 microliters (.mu.l). The volume of the start compartment varies in the same proportions or may be substantially greater than the total amount to be transferred. For example in the embodiment of Figure 4, the chamber 4 has a volume of between 2 and 5 ml for liquid transfer between 250 and 500 .mu.l. The total volume for the fluidic part represented by the channel 8 connecting the starting compartment 4 and the arrival compartment 5 is 120 .mu.l decomposed between 20 .mu.l for the portion of channel 8 connecting the compartment 5 and the distribution channel 11 and 20 .mu.l 5 times for the portion of channel 8 connecting the distribution channel 11 and the compartments 5. the channel 8 in this example is semi-circular section of diameter 0.5 mm. The transfer from another consumable 3 and the valves 15 have already been the subject of a patent application filed by the applicant on 8 September 1998 under the deposit number FR98 / 11383, entitled "Device for reactions, transfer system between devices and implementation process of such a system. " The content of the description of this patent application is considered to be contained in the present invention.

Although inputs 12, outputs 16 and valves 15 are not described in relation to Figures 1 to 3, 5 and 6, it obviously clear that these 1 pumping devices are equipped, even if it is not not shown in the figures, to allow proper operation.

REFERENCES

I. fluid pumping device 2. Sample

3. sealed Consumable

4. First start of said compartment

5. Second compartment says Arrival

6. Shrinkage or channel 7. partition which is deformable or flexible film

8. Primary Channel

9. Canal through

10. Secondary Channel

II. distribution channel 12. Input channel in the consumable 3

13. Apparatus for breaking bubbles

14. Orifice communication between the device 13 and a buffer volume

15. valve

16. Output channel of the consumable 17. 3 inert liquid to actuate the sample 2

F. Force exerting external pressure on the film 7

Claims

1. A pump device (1) for transferring a fluid sample (2) in a sealed consumable (3), the transfer being performed between at least one first compartment, said departure (4) and at least a second compartment, said finish, (5) via a constriction such as a channel (6), characterized in that the intersection point between a start compartment (4) and the constriction is positioned in the lower part and preferably at the lowest level, this starting compartment (4), that the point of intersection between an inlet compartment
(5) and said constriction is positioned in the upper part, and preferably at the highest level, of the inlet chamber (5) and at least one of the starting compartments (4) and / or arrival ( 5) can be deformed, so that each deformation actuates the transfer of all or part of the sample (2).
2. Device according to Claim 1, characterized in that the point of intersection between a starting chamber and the constriction is in contact with the sample, and that the point of intersection between an inlet compartment and said shrinkage is not in contact with said sample.
3. Device according to any one of claims 1 or 2, characterized in that the or from compartments (4) are arranged vertically substantially above or arrival of compartments (5).
4. Device according to any one of claims 1 to 3, characterized in that the intersection point between a start compartment (4) and the constriction (6) is in the upper position relative to the point of intersection between an inlet compartment (5) and said constriction (6).
5. Device according to any one of claims 1 to 4, characterized in that each compartment (4 or 5) which can be deformed has at least one partition which can be deformed (7), such as a flexible film (7) bonded on at least one side of the consumable (1).
6. Device according to any one of claims 1 to 5, characterized in that the consumable is a card that operates in an inclined or vertical position.
7. Device according to any one of claims 1 to 6, characterized in that each constriction is longitudinally driven, in whole or part, by at least one tab which facilitates drainage of the fluid sample.
8. Device according to any one of claims 1 to 7, characterized in that at least one of the compartments is associated with a buffer volume.
9. A method of pumping, characterized in that it consists in deforming at least once a device according to any one of claims 1 to 8.
10. A method according to claim 9, characterized in that it consists in deforming at least one of the starting compartments for applying an overpressure in the gas volume of this compartment, to transfer all or part of the liquid contained by said liquid in at least one inlet chamber.
11. A method according to claim 9, characterized in that it consists in deforming at least one of arrival compartments to create a higher than normal pressure in the gas volume of said compartment in order to transfer all or part of the gas contained by said liquid in at least a start compartment.
12. A method according to claim 9, characterized in that it alternatively comprises:
- deforming at least one of the starting compartments to create a higher than normal pressure in the gas volume of this compartment, to transfer all or part of the liquid contained by said liquid in at least one inlet chamber, and - deforming at any of arrival compartments to create a higher than normal pressure in the gas volume of this compartment, to transfer all or part of the gas contained by said liquid in at least a start compartment.
PCT/FR2000/000580 1999-03-09 2000-03-09 Pumping device for transferring at least a fluid into a consumable WO2000053320A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FR99/03031 1999-03-09
FR9903031A FR2790681B1 (en) 1999-03-09 1999-03-09 pumping device for transferring at least one fluid in a consumable

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2000603803A JP2003517368A (en) 1999-03-09 2000-03-09 Pumping device for moving at least one fluid in consumables
AU32952/00A AU3295200A (en) 1999-03-09 2000-03-09 Pumping device for transferring at least a fluid into a consumable
CA 2362739 CA2362739A1 (en) 1999-03-09 2000-03-09 Pumping device for transferring at least a fluid into a consumable
EP20000910910 EP1159069A1 (en) 1999-03-09 2000-03-09 Pumping device for transferring at least a fluid into a consumable

Publications (1)

Publication Number Publication Date
WO2000053320A1 true WO2000053320A1 (en) 2000-09-14

Family

ID=9543082

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2000/000580 WO2000053320A1 (en) 1999-03-09 2000-03-09 Pumping device for transferring at least a fluid into a consumable

Country Status (6)

Country Link
EP (1) EP1159069A1 (en)
JP (1) JP2003517368A (en)
AU (1) AU3295200A (en)
CA (1) CA2362739A1 (en)
FR (1) FR2790681B1 (en)
WO (1) WO2000053320A1 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003008102A1 (en) * 2001-07-18 2003-01-30 The Regents Of The University Of Michigan Microfluidic gravity pump with constant flow rate
EP1591163A2 (en) * 2003-11-28 2005-11-02 Kabushiki Kaisha Toshiba Nucleic acid detecting cassette, nucleic acid detecting apparatus utilizing nucleic acid detecting cassette, and nucleic acid detecting system utilizing nucleic acid detecting cassette
CN102042954A (en) * 2010-10-22 2011-05-04 重庆大学 Static-pressure bubble measuring method for adhesive force between coated film and base layer
CN102072875A (en) * 2010-10-26 2011-05-25 重庆大学 Geometric measurement method of elastic strain energy in coating films in pressurized blister test
CN102297836A (en) * 2011-05-18 2011-12-28 重庆大学 Direct measurement of one coating interfacial adhesion strength between the film and the base layer

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0192794A1 (en) * 1985-02-27 1986-09-03 Horiba, Ltd. Liquid preserving vessel for use in liquid sample analyzers
US4761381A (en) * 1985-09-18 1988-08-02 Miles Inc. Volume metering capillary gap device for applying a liquid sample onto a reactive surface
WO1994026414A1 (en) * 1993-05-17 1994-11-24 Syntex (U.S.A.) Inc. Reaction container for specific binding assays and method for its use
EP0705978A2 (en) * 1994-10-07 1996-04-10 Bayer Corporation Integral valve diaphragm pump and method
WO1997027324A1 (en) * 1996-01-24 1997-07-31 Sarnoff Corporation Parallel reaction cassette and associated devices
FR2749663A1 (en) * 1996-06-07 1997-12-12 Bio Merieux analysis card single use comprising a conduit for liquids elapsed ly
US5811296A (en) * 1996-12-20 1998-09-22 Johnson & Johnson Clinical Diagnostics, Inc. Blocked compartments in a PCR reaction vessel
JPH1164341A (en) * 1997-08-20 1999-03-05 Hitachi Ltd Chemical analyzing apparatus

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0192794A1 (en) * 1985-02-27 1986-09-03 Horiba, Ltd. Liquid preserving vessel for use in liquid sample analyzers
US4761381A (en) * 1985-09-18 1988-08-02 Miles Inc. Volume metering capillary gap device for applying a liquid sample onto a reactive surface
WO1994026414A1 (en) * 1993-05-17 1994-11-24 Syntex (U.S.A.) Inc. Reaction container for specific binding assays and method for its use
EP0705978A2 (en) * 1994-10-07 1996-04-10 Bayer Corporation Integral valve diaphragm pump and method
WO1997027324A1 (en) * 1996-01-24 1997-07-31 Sarnoff Corporation Parallel reaction cassette and associated devices
FR2749663A1 (en) * 1996-06-07 1997-12-12 Bio Merieux analysis card single use comprising a conduit for liquids elapsed ly
US5811296A (en) * 1996-12-20 1998-09-22 Johnson & Johnson Clinical Diagnostics, Inc. Blocked compartments in a PCR reaction vessel
JPH1164341A (en) * 1997-08-20 1999-03-05 Hitachi Ltd Chemical analyzing apparatus
DE19837434A1 (en) * 1997-08-20 1999-03-11 Hitachi Ltd Chemical analysis unit for quantitative analysis of e.g. biological fluid

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003008102A1 (en) * 2001-07-18 2003-01-30 The Regents Of The University Of Michigan Microfluidic gravity pump with constant flow rate
EP1591163A2 (en) * 2003-11-28 2005-11-02 Kabushiki Kaisha Toshiba Nucleic acid detecting cassette, nucleic acid detecting apparatus utilizing nucleic acid detecting cassette, and nucleic acid detecting system utilizing nucleic acid detecting cassette
EP1591163A3 (en) * 2003-11-28 2006-08-23 Kabushiki Kaisha Toshiba Nucleic acid detecting cassette, nucleic acid detecting apparatus utilizing nucleic acid detecting cassette, and nucleic acid detecting system utilizing nucleic acid detecting cassette
US7135330B2 (en) 2003-11-28 2006-11-14 Kabushiki Kaisha Toshiba Nucleic acid detecting cassette, nucleic and detecting apparatus utilizing nucleic acid detecting cassette, and nucleic acid detecting system utilizing nucleic acid detecting cassette
CN102042954A (en) * 2010-10-22 2011-05-04 重庆大学 Static-pressure bubble measuring method for adhesive force between coated film and base layer
CN102072875A (en) * 2010-10-26 2011-05-25 重庆大学 Geometric measurement method of elastic strain energy in coating films in pressurized blister test
CN102297836A (en) * 2011-05-18 2011-12-28 重庆大学 Direct measurement of one coating interfacial adhesion strength between the film and the base layer

Also Published As

Publication number Publication date
EP1159069A1 (en) 2001-12-05
FR2790681A1 (en) 2000-09-15
FR2790681B1 (en) 2001-05-11
CA2362739A1 (en) 2000-09-14
JP2003517368A (en) 2003-05-27
AU3295200A (en) 2000-09-28

Similar Documents

Publication Publication Date Title
Fu et al. An integrated microfabricated cell sorter
US7384605B2 (en) Fluidics system
US9566581B2 (en) Micro-fluidic device
US9468894B2 (en) Microfluidic mixing and analytical apparatus
Haeberle et al. Microfluidic platforms for lab-on-a-chip applications
CN1973197B (en) A diagnostic system for carrying out a nucleic acid sequence amplification and detection process
US20080038813A1 (en) Sample vessels
AU602469B2 (en) Liquid handling
DE102005019195B4 (en) Chemical reaction cartridge
US7704322B2 (en) Microfluidic free interface diffusion techniques
US9555408B2 (en) Fluid mixing and delivery in microfluidic systems
US9056291B2 (en) Microfluidic reactor system
EP2404676A1 (en) Microfluidic Control Structures
US8383062B2 (en) Fluid processing device including size-changing barrier
US6527003B1 (en) Micro valve actuator
CN101495236B (en) Microfluidic chips and assay systems
EP2093571A2 (en) Automated system for handling microfluidic devices
EP1450954B1 (en) Device for chemical or biochemical analysis
EP2789694A1 (en) Microfluidic device with reaction product recovery system
US9182322B2 (en) Microfluidic mixing and reaction systems for high efficiency screening
US7459127B2 (en) Method and apparatus for precise transfer and manipulation of fluids by centrifugal and/or capillary forces
US7560073B1 (en) Sample support
US20070042427A1 (en) Microfluidic laminar flow detection strip
EP0057110B1 (en) Reaction vessel and method for combining liquids and reagents
CN100394184C (en) Micro-fluid transfer and analysis system

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A1

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
ENP Entry into the national phase in:

Ref country code: CA

Ref document number: 2362739

Kind code of ref document: A

Format of ref document f/p: F

Ref document number: 2362739

Country of ref document: CA

ENP Entry into the national phase in:

Ref country code: JP

Ref document number: 2000 603803

Kind code of ref document: A

Format of ref document f/p: F

WWE Wipo information: entry into national phase

Ref document number: 32952/00

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2000910910

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 09936079

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 2000910910

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWW Wipo information: withdrawn in national office

Ref document number: 2000910910

Country of ref document: EP