WO2000029011A1 - Treatment of tumors by administration of growth hormone releasing compounds and their antagonists - Google Patents
Treatment of tumors by administration of growth hormone releasing compounds and their antagonists Download PDFInfo
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- WO2000029011A1 WO2000029011A1 PCT/EP1999/008662 EP9908662W WO0029011A1 WO 2000029011 A1 WO2000029011 A1 WO 2000029011A1 EP 9908662 W EP9908662 W EP 9908662W WO 0029011 A1 WO0029011 A1 WO 0029011A1
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- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 125000001151 peptidyl group Chemical group 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 210000003635 pituitary gland Anatomy 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000002685 pulmonary effect Effects 0.000 description 1
- MDKCOSRPPDDOPZ-UHFFFAOYSA-N pyrrolo[2,3-g][1]benzazepin-2-amine Chemical compound N1=CC=CC=C2C3=NC(N)=CC3=CC=C21 MDKCOSRPPDDOPZ-UHFFFAOYSA-N 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000003488 releasing hormone Substances 0.000 description 1
- 238000013391 scatchard analysis Methods 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 229940114926 stearate Drugs 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 description 1
- 229940095064 tartrate Drugs 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- WROMPOXWARCANT-UHFFFAOYSA-N tfa trifluoroacetic acid Chemical compound OC(=O)C(F)(F)F.OC(=O)C(F)(F)F WROMPOXWARCANT-UHFFFAOYSA-N 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 239000002676 xenobiotic agent Substances 0.000 description 1
- 230000002034 xenobiotic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/04—Peptides having up to 20 amino acids in a fully defined sequence; Derivatives thereof
- A61K38/08—Peptides having 5 to 11 amino acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/29—Parathyroid hormone, i.e. parathormone; Parathyroid hormone-related peptides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system
- A61P5/10—Drugs for disorders of the endocrine system of the posterior pituitary hormones, e.g. oxytocin, ADH
- A61P5/12—Drugs for disorders of the endocrine system of the posterior pituitary hormones, e.g. oxytocin, ADH for decreasing, blocking or antagonising the activity of the posterior pituitary hormones
Definitions
- the invention relates to a method for reducing the proliferation of carcinoma cells by administration of growth hormone releasing peptides and antagonists thereof.
- GHRH GH-releasing hormone
- GHRP GH-releasing peptides
- GHRPs and their antagonists are described, for example, 5 in the following publications: C.Y. Bowers, supra, R. Deghenghi, "Growth Hormone Releasing Peptides", ibidem, 1996, pg. 85-102; R. Deghenghi et al . , “Small Peptides as Potent Releasers of Growth Hormone", J. Ped. End. Metab., 8, pg. 311-313, 1996; R. Deghenghi, "The Development of
- Impervious Peptides as Growth Hormone Secretagogues Acta Paediatr. Suppl . , 423, pg. 85-87, 1997; K. Veeraraganavan et al., "Growth Hormone Releasing Peptides (GHRP) Binding to Porcine Anterior Pituitary and Hypothalamic Membranes", Life Sci . , 50, Pg. 1149- 1155,
- the present invention relates to a method for treating a tumor in a mammal which method comprises administering to a mammal in need of such treatment an effective amount of a growth hormone releasing peptide (GHRP) or an 5 antagonist thereof.
- GHRP growth hormone releasing peptide
- the compounds used according to the invention can be defined as growth hormone secretagogues or antagonists thereof.
- the amounts of these compounds are effective to reduce or inhibit the proliferation of tumorigenic cells in the mammal.
- these compounds are specified by the feature that they displace the radioactive marker 125 I-Tyr-Ala-His-D-Mrp-Ala-Trp-D-Phe-Lys-NH ?
- treated tumors are lung, mammary, thyroid or pancreas tumors .
- the above mentioned compounds include certain known compounds (cf. above), but other compounds useful in the invention are not previously published and include a spirolactam, bicyclic or tricyclic peptidomimetic unit.
- One common feature for all compounds useful in the invention is that at least one lysine unit is present.
- Figure 1 is a graph which illustrates the specific binding of 125 I-Tyr-Ala-Hexarelin to membranes from different non-endocrine and endocrine human tumors of various origins.
- Figure 2 is a graph which illustrates the 125 I-Tyr-Ala- Hexarelin binding to membranes from a non-endocrine lung tumor .
- Figure 3 is a graph which illustrates the displacement of 125 I-Tyr-Ala-Hexarelin to membranes from non-endocrine lung tumor membranes by various compounds.
- the ordinate represents binding as a percentage of control (i.e. specific binding in the absence of unlabelled competitor) .
- Figure 4 is a graph which illustrates the effect of Hexarelin, Ala-Hexarelm, Tyr-Ala-Hexarelm, EP80317 (HAIC-D-Mrp-D-Lys-Trp-D-Phe-Lys-NH 2 ) , D- (Lys) ,-GHRP6 (His- D-Trp-D-Lys-Trp-D-Phe-Ly ⁇ -NH 2 ) and MK0677 (N-[l (R) ([1, 2- d ⁇ hydro-1-methanesulfonylsp ⁇ ro- (3H- ⁇ ndole, 3, 4 - pipe ⁇ dm) -1 'yl]-2- (phenylmethoxy) ethyl]-2-ammo- methylpropanamide-methanesulfonate) on basal and EGF- stimulated 3 H-thym ⁇ dme incorporation in human lung carcinoma cells.
- Figure 5 is a graph which illustrates the effect of Hexarelin, Ala-Hexarelm, Tyr-Ala-Hexarelm, EP80317, D- (Lys) 3 -GHRP6 and MK0677 on EGF-stimulated 3 H-thym ⁇ dme incorporation in human lung carcinoma cells shown as dose responsive curves.
- Figure 6 is a graph which illustrates the effect of Hexarelin on human lung carcinoma cell proliferation.
- Figure 7 is a graph which illustrates the effect of Hexarelin (a) and Ala-Hexarelm (b) on human breast cancer (T47D) cell proliferation.
- Figure 8 Effect of Hexarelin (a) and Ala-Hexarelm (b) on human breast cancer (MDA-MB231) cell proliferation.
- D is the dextro enantiomer
- GH is growth hormone
- Mrp is 2-Methyl-Trp
- IMA is lmidazolylacetyl
- GAB is ⁇ - ammo butyryl
- INIP is isopecotinyl
- AIB is ammo isobutyryl
- Nal is ⁇ -naphthylalanme
- TXM is tranexamyl, i.e.
- D-HNH is D- 1, 2, 3, 4, 5, 6-hexahydro-norharman-3-carboxylate
- HAIC is (2S, 5S) -5-am ⁇ no-l, 2,4,5,6, 7-hexahydro-azepmo[3, 2,1- h ⁇ ]mdole-4-one-2-carboxylate
- ATAB is 2-R- (2 ⁇ , 5 ⁇ , 8 ⁇ ) -8- am ⁇ no-7-oxo-4-th ⁇ a-l-aza-b ⁇ cyclo[3.4.0]nonan-2- carboxylate
- Ala, Lys, Phe, Trp, His, Thr, Cys, Tyr, Leu and lie are the ammo acids alanme, lysine, phenylalan e, tryptophan, histidine, threonme, cysteme, tyrosme, leucme and isoleucme, respectively.
- useful compounds to be administered are of the general formula I:
- AA 1 is IMA, GAB, INIP, TXM, AIB, HIs-D-Trp-, His-D-Mrp,
- Trp Trp, IMA-D-Mrp,
- AA 3 is D-Trp, D-Nal, D-Trp, Mrp, D-Mrp, Phe, or D-Phe;
- AA 4 is D-Trp, Mrp, D-Mrp, Phe, or D-Phe; and R is -NH 2 , Thr-NH 2 , or D-Thr-NH 2 .
- co punds containing a D-Mrp unit are preferred.
- the useful compounds include those described in U.S. patent application no . 09/089,954, filed June 3, 1998. These compounds are peptides of the general formula II:
- R 1 is H or Tyr
- R 2 represents the side chain of any one naturally occurring amino acid, and the configuration at * is (R) , (S) or a mixture thereof; a tricyclic compound of the formula IV
- R ⁇ is H or Tyr and the configuration at * is (R) (S) or a mixture thereof; a bicyclic compound of the formula V H
- R 4 is H or Tyr and the configuration at * is (R) , (S) or a mixture thereof;
- D-Mrp is Dextro-2-Methyl-Trp;
- C is Trp-Phe-Lys, D-Trp-Phe-Lys, Mrp-Phe-Lys, D-Mrp-Phe- Lys, Trp-Lys,
- D-Trp-Ly ⁇ Mrp-Lys, D-Mrp-Lys, Ala-Trp-D-Phe-Lys, Ala- Mrp-D-Phe-Lys , Ala-D-Mrp-D-Phe-Lys, D-Lys-Trp-D-Phe-Lys, D-Lys-Mrp-D- Phe-Lys,
- R b is H or S0 2 Me and the configurations at * are either (R) , (S) or a mixture thereof; and E is Lys-NH 2 or -NH 2 , provided that E is Lys-NH 2 , when C is the previously defined tricyclic compound VI.
- the tumor to be treated according to the invention is a lung, mammary, thyroid or pancreas tumor .
- GH liberating compounds of the general formula I include the following:
- While preferred compounds of the general formula I that do not liberate GH include: Hi ⁇ -D-Trp-D-Lys-Trp-D-Phe-Lys-NH 2 , His-D-Mrp-D-Lys-Trp-D-Phe-Lys-NH 2 , His-Ala-D-Trp-Lys-Mrp-D-Phe-Lys-NH 2 , His-D-Mrp-D-Lys-Mrp-D-Phe-Lys-NH 2 , His-Ala-D-Trp-Ala-Mrp-D-Phe-Lys-NH 2 , and His-D-Trp-Ala-Mrp-D-Phe-Lys-NH 2 .
- the preferred compounds of the general formula II include the following: [S, S-Spiro (Pro-Leu) ]-D-Mrp-D-Trp-Phe-Lys-NH 2 , [S, S-Spiro (Pro-Leu) ]-D-Mrp-Mrp-Lys-NH 2 , [S, S-Spiro (Pro-Leu) ]-D-Mrp-Ala-Trp-D-Phe-Lys-NH 2 , [S, S-Spiro (Pro-Leu) ]-D-Mrp-D-Lys-Trp-D-Phe-Lys-NH 2 , Tyr-[S, S-Spiro (Pro-Leu) ]-D-Mrp-D-Lys-Trp-D-Phe-Lys-NH 2 , [S, S-Spiro (Pro-He) ]-D-Mrp-D-Lys-Tr
- R ⁇ is H and R 2 is the side chain of Leu or lie (see P. Ward et al., J. Med. Chem., 33, 1848 (1990)).
- the tricyclic compound HNH is obtained by conventional hydrogenation of the corresponding tetrahydro-norharman- 3-carboxyl ⁇ c acids of the formula
- the units according to the formulas III, IV, V and VI constitute peptidomimetic units which are advantageous in that they lock in a ⁇ -turn configuration thus mimicking natural ammo acids.
- salts of these compounds can be also used, if desired.
- Such salts include organic or inorganic addition salts, such as hydrochloride, hydrobromide, phosphate, sulfate, acetate, succ ate, ascorbate, tartrate, gluconate, benzoate, malate, fumarate, stearate or pamoate salts.
- All compounds can be conveniently synthesized according to the usual methods of peptide chemistry, such as by solid-phase peptide synthesis, as described by E. Atherton and R.C. Sheppard m “Solid Phase Peptide Synthesis", IRL Press at Oxford University Press, 1989, by solution-phase synthesis as described by J. Jones m “The Chemical Synthesis of Peptides”, Clarendon Press, Oxford 1994, or by a combination of both solid- and solution-phase methods, as known in the art.
- the solid-phase synthesis starts from the C-terminal end of the compounds.
- a suitable starting material can be prepared, for example, by attaching the required protected ⁇ -amino acid to a chloromethylated resin, a hydroxymethylated resin, a benzhydrylamine resin (BHA) , or to a para-methyl-benzhydrylamine resin (p-Me-BHA) .
- a chloromethylated resin is BIOBEADS SX1 by BioRad Laboratories, Richmond, California.
- the preparation of the hydroxymethylated resin is described by Bodansky et al., Chem. Ind. (London), 38, 15997 (1966).
- the BHA resin is described by Pietta and Marshall, Chem. Comm., 650 (1970), and is commercially available by Belmont, California.
- the protecting group of the ⁇ -amino acid can be removed by means of different acid reagents, such as trifluoroacetic acid (TFA) or hydrochloric acid (HCl) dissolved in organic solvents at room temperature.
- TFA trifluoroacetic acid
- HCl hydrochloric acid
- the remaining protected natural amino acids or carboxylic acids corresponding to the units according to the general formulas III, IV, V and VI, which also constitute amino acids can be coupled step by step in the desired order.
- Each protected amino acid can generally be reacted in excess of about three times using a suitable carboxyl activating group, such as dicyclohexylcarbodiimide (DCC) or diisopropylcarbodiimide (DIC) dissolved, for example, in ethylene chloride
- a suitable carboxyl activating group such as dicyclohexylcarbodiimide (DCC) or diisopropylcarbodiimide (DIC) dissolved, for example, in ethylene chloride
- the desired compound can be cleaved from the supporting resin by treatment with a reagent such as hydrogen fluoride (HF) which cleaves not only the compound from the resm, but also the protecting groups of lateral chains.
- a reagent such as hydrogen fluoride (HF) which cleaves not only the compound from the resm, but also the protecting groups of lateral chains.
- HF hydrogen fluoride
- a chloromethylated resin treatment with HF leads to the formation of a compound which has a terminal acid group and is present in free form.
- a BHA or p-Me-BHA resin the treatment with HF directly leads to the formation of a compound which has a terminal amide group and is present in free form.
- Medicaments useful for treating tumors m a mammal, including a human can comprise a compound according to the present invention or a pharmaceutically acceptable salt thereof, or combinations of compounds according to the present invention or pharmaceutically acceptable salts thereof, optionally in admixture with a carrier, excipient, vehicle, diluent, matrix, or delayed release coating.
- a carrier excipient, vehicle, diluent, matrix, or delayed release coating.
- any of the compounds according to the present invention can be formulated by the skilled in the art to provide medicaments which are suitable for parenteral, buccal, rectal, vaginal, transdermal, pulmonary or oral routes of administration.
- the type of formulation of the medicament containing the compound can be selected according to the desired rate of delivery. For example, if the compounds are to be rapidly delivered, the nasal or intravenous route is preferred.
- the medicaments can be administered to mammals, including humans, at a therapeutically effective dose which can be easily determined by one of skill in the art and which can vary according to the specie, age, sex and weight of the treated patient or subject as well the route of administration. For example, in humans, when intravenously administered, the preferred dose falls in the range from about 1 ⁇ g to about 25 ⁇ g of total compound per kg of body weight. When orally administered, higher amounts are generally necessary. For example, in humans for the oral administration, the dosage level is typically from about 30 ⁇ g to about 1000 ⁇ g of total compound per kg of body weight. The exact level can be easily determined empirically based on the above disclosure.
- Hexarelin His-D-Mrp-Ala-Trp-D-Phe-Lys-NH 2
- Ala- Hexarelin Ala- Hexarelin
- Ala-Hexarelin Ala- Hexarelin
- Tyr-Ala- Hexarelin Tyr-Ala-His-D-Mrp-Ala-Trp-D-Phe-Lys-NH 2
- MK0677 N-[l (R) ([1 , 2-dihydro-l-methanesulfonylspiro- ( 3H- indole, 3,4 ' -piperidin)-l ' yl]-2- (phenylmethoxy) ethyl]-2- amino- ethylpropanamide-methanesulfonate)
- EP80317 HAIC- D-Mrp-D-Lys-Trp-D-Phe-Lys-NH 2
- D- (Lys) 3 -GHRP6 His-D- Trp-D-Lys-Trp-D-Phe-Lys-NH 2 ) were supplied by Europeptides (Argenteuil, France) .
- GHRH Human GHRH (GHRH 1-44) and somatostatin (somatostatin 1-14) were purchased from Bachem (Bubendorf, Switzerland) .
- Surgical tumor specimens were collected from the Department of Biomedical Sciences and Human Oncology (Division of Pathology) of the University of Turin. A tumor fragment adjacent to that used for histopathological diagnosis was immediately frozen at -80 °C and stored for 2 to 60 months until further processed for binding studies. Samples of 13 invasive breast carcinoma (10 ductal and 3 lobular) , 14 non-endocrine lung carcinomas (5 squamous cell and 9 adenocarcinomas) , 11 endocrine tumors of the lung, 9 endocrine tumors of the pancreas and 12 thyroid carcinomas (7 of follicular origin and 5 of medullary origin) were used. Non- neoplastic normal tissues of the corresponding organs were also analysed in parallel with the individual tumors .
- Human lung carcinoma cells (CaLul), T47D and MDA-MB231, respectively, human oestrogen dependent and oestrogen independent breast cancer cell lines were purchased from the ATCC (Rockville, MD, USA) . Cells were routinely cultured in 25 cm 3 flasks at 37 °C, 5% C0 2 and 95% humidified atmosphere in RPMI supplemented with 10% FCS, penicillin-streptomycin and fungizone. When a subconfluent state was reached, cells were detached from the flasks with trypsin/EDTA.
- GHRP receptors were measured on tumor membranes as described in G. Muccioli et al., Journal of Endocrinology, 157, 99-106, 1998, using 125 I-Tyr-Ala- Hexarelin as a ligand. Specific binding was calculated as the difference between binding in the absence and in the presence of excess unlabelled Tyr-Ala-Hexarelin and expressed as a percentage of the radioactivity added. Saturation and competition binding studies were analyzed with the GraphPAD Prism 2 program (GraphPAD Software, San Diego, CA, USA) .
- Figure 1 shows the distribution of radiolabelled Tyr-Ala- 30 Hexarelin binding to membranes from different endocrine and non-endocrine human tumors of various origins (*P ⁇ 0.01 vs. the corresponding non-tumoral tissue).
- Non- endocrine tumors of the lung and breast, as well endocrine carcinomas of the pancreas and thyroid (follicular type) showed a median specific binding value which was statistically higher than that found in the corresponding non tumoral normal tissue.
- no difference in the specific binding values was observed between normal tissue and endocrine tumors of the lung or thyroid (medullary type) .
- the specificity of 125 I-Tyr-Ala-Hexarelin binding was established by determining the ability of different compounds to compete with the radioligand for the tumoral binding sites (cf . Fig. 3) .
- the binding of radiotracer was displaced in a dose-dependent fashion by Hexarelin, Ala-Hexarelin, Tyr-Ala-Hexarelin and GHRP antagonists such as D- (Lys) 3 -GHRP6 and EP 80317, an (Amino-azepino- indol) ]-D- (Lys) 3 derivative of Hexarelin which does not release GH in neonatal rats.
- Hexarelin at 10-6 mol/1 was able to inhibit both basal and the EGF-stimulated 3 H-thymidine incorporation in human cells of lung carcinoma (cf. Fig. 4; *P ⁇ 0.05,
- the EC 5 o value was 5.6 x IO "8 mol/1 for EP80317, 6.5 x IO "8 mol/1 for Tyr-Ala-Hexarelin, 8 x IO "8 mol/1 for Hexarelin, 9 x IO "8 mol/1 for (D-Lys) 3 - GHRP6 and 1 x 10 "7 mol/1 for Ala-Hexarelin.
- Hexarelin at IO " mol/1 caused a decrease in cell number compared with the control with a significant effect (-47%) only after 96 hours. This effect further increased at 10 " ' mol/1 and 10 " 6 mol/1 and was observed at any time point tested (cf. Fig. 6; **p ⁇ 0.001; ***P ⁇ 0.0001 vs. control).
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Abstract
Description
Claims
Priority Applications (17)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA002350914A CA2350914A1 (en) | 1998-11-16 | 1999-11-11 | Treatment of tumors by administration of growth hormone releasing compounds and their antagonists |
NZ511280A NZ511280A (en) | 1998-11-16 | 1999-11-11 | Treatment of tumors by administration of growth hormone releasing compounds and their antagonists |
DE69914365T DE69914365T2 (en) | 1998-11-16 | 1999-11-11 | USE OF GROWTH HORMONE RELEASING SUBSTANCES AND THEIR ANTAGONISTS FOR TREATING TUMORS |
EP99955974A EP1131083B1 (en) | 1998-11-16 | 1999-11-11 | Use of growth hormone releasing compounds and their antagonists for the treatment of tumors |
AU12706/00A AU768516B2 (en) | 1998-06-03 | 1999-11-11 | Treatment of tumors by administration of growth hormone releasing compounds and their antagonists |
JP2000582057A JP2002529512A (en) | 1998-11-16 | 1999-11-11 | Treatment of tumors by administration of growth hormone releasing compounds and their antagonists |
SK635-2001A SK6352001A3 (en) | 1998-11-16 | 1999-11-11 | Treatment of tumors by administration of growth hormone releasing compounds and their antagonists |
MXPA01004689A MXPA01004689A (en) | 1998-11-16 | 1999-11-11 | Treatment of tumors by administration of growth hormone releasing compounds and their antagonists. |
IL14285899A IL142858A0 (en) | 1998-11-16 | 1999-11-11 | Treatment of tumors by administration of growth hormone releasing compounds and their antagonists |
AT99955974T ATE258062T1 (en) | 1998-11-16 | 1999-11-11 | USE OF GROWTH HORMONE RELEASING SUBSTANCES AND THEIR ANTAGONISTS FOR THE TREATMENT OF TUMORS |
DK99955974T DK1131083T3 (en) | 1998-11-16 | 1999-11-11 | Use of growth hormone releasing compounds and antagonists thereof for the treatment of tumors |
BR9915390-4A BR9915390A (en) | 1998-11-16 | 1999-11-11 | Treatment of tumors through administration of compounds that release growth hormone and its antagonists |
HU0104374A HUP0104374A3 (en) | 1998-11-16 | 1999-11-11 | Growth hormone releasing compounds and their antagonists for use for treatment of tumors |
UA2001064044A UA73100C2 (en) | 1998-11-16 | 1999-11-11 | Method for inhibiting proliferation of cancer cells by administering growth hormone releasing compound or its antagonist |
NO20012367A NO20012367L (en) | 1998-11-16 | 2001-05-14 | Treatment of tumors by administration of growth hormone releasing compounds and their antagonists |
BG105572A BG105572A (en) | 1998-11-16 | 2001-06-07 | Treatment of tumors by administration of growth hormone releasing compounds and their antagonists |
HK02102769.3A HK1040934A1 (en) | 1998-11-16 | 2002-04-12 | Treatment of tumors by administration of growth hormone releasing compounds and their antagonists |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/192,406 US6124263A (en) | 1998-11-16 | 1998-11-16 | Treatment of tumors by administration of growth hormone releasing compounds and their antagonists |
US09/192,406 | 1998-11-16 |
Publications (1)
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WO2000029011A1 true WO2000029011A1 (en) | 2000-05-25 |
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Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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PCT/EP1999/008662 WO2000029011A1 (en) | 1998-06-03 | 1999-11-11 | Treatment of tumors by administration of growth hormone releasing compounds and their antagonists |
Country Status (30)
Country | Link |
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US (1) | US6124263A (en) |
EP (1) | EP1131083B1 (en) |
JP (1) | JP2002529512A (en) |
KR (1) | KR100704138B1 (en) |
CN (3) | CN100368014C (en) |
AR (1) | AR021286A1 (en) |
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Cited By (7)
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WO2002090387A1 (en) * | 2001-05-10 | 2002-11-14 | Queensland University Of Technology | Reproductive cancer diagnosis and therapy |
WO2005027913A1 (en) * | 2003-09-19 | 2005-03-31 | Pfizer Products Inc. | Pharmaceutical compositions and methods comprising combinations of 2-alkylidene-19-nor-vitamin d derivatives and a growth hormone secretagogue |
EP1542716A1 (en) * | 2002-08-09 | 2005-06-22 | Societe De Conseils De Recherches Et D'applications Scientifiques S.A.S. | Growth hormone releasing peptides |
WO2007127457A2 (en) * | 2006-04-28 | 2007-11-08 | The Administrators Of The Tulane Educational Fund | Ghrelin/growth hormone releasing peptide/growth hormone secretatogue receptor antagonists and uses thereof |
WO2008039415A2 (en) | 2006-09-27 | 2008-04-03 | Ipsen Pharma S.A.S. | Analogs of ghrelin substituted at the n-terminal |
ITMI20100118A1 (en) * | 2010-01-28 | 2011-07-29 | Uni Degli Studi Di Milano B Icocca | USE OF RECEPTOR AGONISTS AND ANTAGONISTS FOR GROWTH HORMONE SECRETAGOGUES FOR THE PREVENTION AND TREATMENT OF CONVULSIONS AND EPILEPSY |
US9724381B2 (en) | 2009-05-12 | 2017-08-08 | The Administrators Of The Tulane Educational Fund | Methods of inhibiting the ghrelin/growth hormone secretatogue receptor pathway and uses thereof |
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US6211156B1 (en) * | 1999-11-10 | 2001-04-03 | Asta Medica A.G. | Peptides for treatment of erectile dysfunction |
CU23157A1 (en) * | 2001-01-03 | 2006-07-18 | Ct Ingenieria Genetica Biotech | PHARMACEUTICAL COMPOSITION FOR THE TREATMENT OF TISSULAR DANE DUE TO LACK OF BLOOD IRRIGATION ARTERIAL |
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EP1312363A1 (en) * | 2001-09-28 | 2003-05-21 | Pfizer Products Inc. | Methods of treatment and kits comprising a growth hormone secretagogue |
US20050164952A1 (en) * | 2004-01-23 | 2005-07-28 | Vital Pharmaceuticals, Inc. | Delivery system for growth hormone releasing peptides |
JP2010518158A (en) * | 2007-02-13 | 2010-05-27 | ヘルシン セラピューティクス(ユー.エス.),インコーポレイティド | Method for treating cell proliferative disorder using growth hormone secretagogue |
WO2010118309A2 (en) * | 2009-04-10 | 2010-10-14 | Board Of Regents, The University Of Texas System | Inhibitors of stat3 and uses thereof |
EP3920961A4 (en) | 2019-02-08 | 2022-12-14 | United States Government as represented by the Department of Veterans Affairs | Growth hormone-releasing hormone antagonists and uses thereof |
WO2021011874A1 (en) * | 2019-07-18 | 2021-01-21 | University Of Miami | Ghrh antagonists for use in a method of treating sarcoidosis |
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- 1999-11-11 CZ CZ20011577A patent/CZ20011577A3/en unknown
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- 1999-11-11 RU RU2001116722/14A patent/RU2240133C2/en not_active IP Right Cessation
- 1999-11-11 DK DK99955974T patent/DK1131083T3/en active
- 1999-11-11 UA UA2001064044A patent/UA73100C2/en unknown
- 1999-11-11 WO PCT/EP1999/008662 patent/WO2000029011A1/en active IP Right Grant
- 1999-11-11 PL PL99347659A patent/PL347659A1/en unknown
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WO2002090387A1 (en) * | 2001-05-10 | 2002-11-14 | Queensland University Of Technology | Reproductive cancer diagnosis and therapy |
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US8536120B2 (en) | 2006-04-28 | 2013-09-17 | The Administrators Of The Tulane Educational Fund | Ghrelin/growth hormone releasing peptide/growth hormone secretatogue receptor antagonists and uses thereof |
WO2007127457A3 (en) * | 2006-04-28 | 2008-02-21 | Univ Tulane | Ghrelin/growth hormone releasing peptide/growth hormone secretatogue receptor antagonists and uses thereof |
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US9724381B2 (en) | 2009-05-12 | 2017-08-08 | The Administrators Of The Tulane Educational Fund | Methods of inhibiting the ghrelin/growth hormone secretatogue receptor pathway and uses thereof |
ITMI20100118A1 (en) * | 2010-01-28 | 2011-07-29 | Uni Degli Studi Di Milano B Icocca | USE OF RECEPTOR AGONISTS AND ANTAGONISTS FOR GROWTH HORMONE SECRETAGOGUES FOR THE PREVENTION AND TREATMENT OF CONVULSIONS AND EPILEPSY |
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