WO2000026410A1 - Procedes de prevention de la contamination croisee dans les dosages a support solide - Google Patents

Procedes de prevention de la contamination croisee dans les dosages a support solide

Info

Publication number
WO2000026410A1
WO2000026410A1 PCT/US1999/025653 US9925653W WO2000026410A1 WO 2000026410 A1 WO2000026410 A1 WO 2000026410A1 US 9925653 W US9925653 W US 9925653W WO 2000026410 A1 WO2000026410 A1 WO 2000026410A1
Authority
WO
Grant status
Application
Patent type
Prior art keywords
solid
support
reagent
solution
assay
Prior art date
Application number
PCT/US1999/025653
Other languages
English (en)
Inventor
Paul V. Haydock
Jason D. Ray
Original Assignee
Saigene Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

L'invention concerne un appareil et des procédés visant à la réduction d'artefacts dans les dosages dans lesquels un support solide est utilisé, grâce à la réduction du report de réactifs d'un mélange de dosage à un autre. L'invention peut être utile dans les techniques d'analyse suivantes: les dosages en sandwich, y compris les dosages d'hybridation de l'acide nucléique, et les dosages immunologiques.
PCT/US1999/025653 1998-11-03 1999-11-02 Procedes de prevention de la contamination croisee dans les dosages a support solide WO2000026410A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10685798 true 1998-11-03 1998-11-03
US60/106,857 1998-11-03

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CA 2349635 CA2349635A1 (fr) 1998-11-03 1999-11-02 Procedes de prevention de la contamination croisee dans les dosages a support solide
EP19990971466 EP1127167A4 (fr) 1998-11-03 1999-11-02 Procedes de prevention de la contamination croisee dans les dosages a support solide

Publications (1)

Publication Number Publication Date
WO2000026410A1 true true WO2000026410A1 (fr) 2000-05-11

Family

ID=22313637

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/025653 WO2000026410A1 (fr) 1998-11-03 1999-11-02 Procedes de prevention de la contamination croisee dans les dosages a support solide

Country Status (3)

Country Link
EP (1) EP1127167A4 (fr)
CA (1) CA2349635A1 (fr)
WO (1) WO2000026410A1 (fr)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4018734A (en) * 1974-06-10 1977-04-19 Rhone-Poulenc Industries Organosilicon compositions which impart non-stick properties to cellulosic and synthetic materials
US4994373A (en) * 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US5556748A (en) * 1991-07-30 1996-09-17 Xenopore Corporation Methods of sandwich hybridization for the quantitative analysis of oligonucleotides

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554088A (en) * 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
US4921809A (en) * 1987-09-29 1990-05-01 Findley Adhesives, Inc. Polymer coated solid matrices and use in immunoassays

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4018734A (en) * 1974-06-10 1977-04-19 Rhone-Poulenc Industries Organosilicon compositions which impart non-stick properties to cellulosic and synthetic materials
US4994373A (en) * 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US5556748A (en) * 1991-07-30 1996-09-17 Xenopore Corporation Methods of sandwich hybridization for the quantitative analysis of oligonucleotides

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FALIPOU S. ET AL.: "New use of cyanosilane coupling agent for direct binding of antibodies to Silica supports. Physicochemical characterization of molecularly bioengineered layers", BIOCONJUGATE CHEMISTRY, vol. 10, no. 3, March 1999 (1999-03-01), pages 346 - 353, XP002926876 *
See also references of EP1127167A4 *

Also Published As

Publication number Publication date Type
EP1127167A4 (fr) 2004-07-14 application
EP1127167A1 (fr) 2001-08-29 application
CA2349635A1 (fr) 2000-05-11 application

Similar Documents

Publication Publication Date Title
US4591570A (en) Matrix of antibody-coated spots for determination of antigens
US5578577A (en) Method for storing labile proteins
EP0262328A2 (fr) Bande d'essay chromatographique pour la détermination de ligands et de récepteurs
US4315907A (en) Combined heterogeneous specific binding assay
US5206177A (en) Apparatus for determining an analyte and method therefor
US20020052009A1 (en) Polyethylene glycol-derivatized biomolecules and their use in heterogeneous detection methods
US6703196B1 (en) General purpose structure of an analytical element and its application for analyte determination
US5541069A (en) Assay having improved dose response curve
US5556748A (en) Methods of sandwich hybridization for the quantitative analysis of oligonucleotides
Hendrickson et al. High sensitivity multianalyte immunoassay using covalent DNA-labeled antibodies and polymerase chain reaction
US5824478A (en) Diagnostic methods and probes
US5879881A (en) Solid phase system for use in ligand-receptor assays
US5308775A (en) Assay devices for concurrently detecting an analyte and confirming the test result
US20090088332A1 (en) Multiplex Digital Immuno-Sensing Using a Library of Photocleavable Mass Tags
US4960692A (en) Assay employing binding pair members on particles and on a filter or membrane
US5354654A (en) Lyophilized ligand-receptor complexes for assays and sensors
US5422284A (en) Method of performing affinity separation using immobilized flocculating agent on chromatographic support
US4496654A (en) Detection of HCG with solid phase support having avidin coating
US4378344A (en) Method and apparatus for performing multiple, simultaneous in vitro diagnostic tests using a solid phase system
US5648213A (en) Compositions and methods for use in detection of analytes
US5171537A (en) Activated immunodiagnostic pipette tips
US5635362A (en) Assay of blood or other biologic samples for target analytes
US6682648B1 (en) Electrochemical reporter system for detecting analytical immunoassay and molecular biology procedures
US6117631A (en) Detection of antigens via oligonucleotide antibody conjugates
US5480792A (en) Antibodies to complexes of ligand receptors and ligands and their utility in ligand-receptor assays

Legal Events

Date Code Title Description
ENP Entry into the national phase in:

Ref country code: AU

Ref document number: 2000 14615

Kind code of ref document: A

Format of ref document f/p: F

AL Designated countries for regional patents

Kind code of ref document: A1

Designated state(s): GH GM KE LS MW SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

AK Designated states

Kind code of ref document: A1

Designated state(s): AE AL AM AT AU AZ BA BB BG BR BY CA CH CN CR CU CZ DE DK DM EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
ENP Entry into the national phase in:

Ref country code: CA

Ref document number: 2349635

Kind code of ref document: A

Format of ref document f/p: F

Ref document number: 2349635

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 14615/00

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 1999971466

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 09830976

Country of ref document: US

WWP Wipo information: published in national office

Ref document number: 1999971466

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWG Wipo information: grant in national office

Ref document number: 14615/00

Country of ref document: AU

WWW Wipo information: withdrawn in national office

Ref document number: 1999971466

Country of ref document: EP