WO2000011200A2 - Plant expression vectors - Google Patents
Plant expression vectors Download PDFInfo
- Publication number
- WO2000011200A2 WO2000011200A2 PCT/US1999/019102 US9919102W WO0011200A2 WO 2000011200 A2 WO2000011200 A2 WO 2000011200A2 US 9919102 W US9919102 W US 9919102W WO 0011200 A2 WO0011200 A2 WO 0011200A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- gene
- isolated
- wheat
- rice
- nucleotide sequence
- Prior art date
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Definitions
- non-translated genetic elements that are capable of enhancing expression can minimize some of the rate-limiting steps in transgenic plant production.
- Fig. 14 illustrates plasmid pMON32517
- Fig. 20 illustrates plasmid pMON47906
- Particularly preferred plants include alfalfa, barley, corn, cotton, canola, potato, rice, rye, soybean, sunflower, sugarbeet. and wheat. Even more preferred plants include monocots such as corn, wheat, and rice.
- PMON26054 ( Figure 4) was created by subcloning the 0.449 kb Stul and Ncol fragment containing the rice actin intron (McElroy, et. al., 1991 ) from pMON25454 into the Bglll and Ncol sites of pMON26045 using adaptors to the Stul site which create a Bglll complementary end (SEQ ID NO. 3 and SEQ ID NO. 4).
- the resulting transformants with inserts from 3 * non-translated regions of wheat genes were verified by DNA sequencing.
- the pMON32502 ( Figure 6) expression vector was constructed by digestion of pMON26044 and pMON 18375 with EcoRI and Smal.
- the 0.24 kb Ta hsp 3' fragment from pMON 18375 was ligated with the 6.0 kb vector backbone fragment of pMON26044 creating pMON32502.
- the ligation products were transformed into E. coli DH5 alpha cells by standard procedures, plated on LB agar plates containing selective levels of ampicillin ( 100 ⁇ g/ml).
- PMON32510 ( Figure 9) was constructed by digestion of pMON26055 ( Figure 3) and pMON18377 with EcoRI and Smal. The 0.23 kb Ta ubiq 3' fragment from pMON18377 was ligated with the 5.9 kb vector backbone fragment of pMON26055 creating pMON32510 ( Figure 9). The ligation products were transformed into E. coli DH5 alpha cells by standard procedures.
- the first intron of the rice amylase gene (r amyl intron) (Genebank Accession Number X 16509. gb_pl) (SEQ ID NO:49) along with 10 base pairs of flanking 5' and 3' exon sequence was isolated by PCR of rice (Oryza sativa) genomic DNA, using about 1 ⁇ g DNA. Amplification was performed using primer sequences SEQ ID NO:29 and SEQ ID NO:30. The DNA was denatured for 1 minute at 95 °C. annealed for 2 minutes at 50 °C, and extended for 3 minutes at 72 °C for a total of 30 cycles. The resultant PCR product was digested with Bglll and Xbal and ligated with the 5.687 kb Bglll and Xbal fragment of pMON33210 ( Figure 16) to create pMON3321 1.
- CM4C Raff/mann medium 1.95 g/L MES, 2 g/L Phytagel
- cultures were transferred to CM4C Raff/mann medium for about 4 days, bombarded and transferred to CM4C containinng 25 mg/L G418 for about 5 days; cultures were regenerated on MMS0.2C containing G418 (25 mg/L) for about 19 days, regenerated on MMSOC containing 25 mg/L G418 for about 33 days and rooted on MMSOC containing 25 mg L G418 for about 57 days and subsequently transferred to soil at about 75 days.
- Relative GUS levels were comparable or greater than the base control vector for many of the constructs containing the non-translated elements of the invention.
- constructs containing a Ta cab or Ta fbp 5 " non-translated leader sequence used in combination with a Ta hsp or Ta fbp 3 ' non- translated terminator sequence provided the highest expression levels.
- Another preferred construct contained a Ta fbp 5 ' non-translated leader and a 3 ' non-translated nos terminator region.
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Botany (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Insects & Arthropods (AREA)
- Pest Control & Pesticides (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL99346650A PL346650A1 (en) | 1998-08-19 | 1999-08-18 | Plant expression vectors |
BRPI9913690A BRPI9913690B1 (en) | 1998-08-19 | 1999-08-18 | recombinant DNA molecule, transgenic microorganism cell, and method for providing enhanced gene expression in plants |
DE69943375T DE69943375D1 (en) | 1998-08-19 | 1999-08-18 | VEGETABLE EXPRESSION VECTORS |
CA2340286A CA2340286C (en) | 1998-08-19 | 1999-08-18 | Plant expression vector comprising a 5' non-translated leader sequence from a wheat chlorophyll a/b-binding protein gene |
AT99942392T ATE506441T1 (en) | 1998-08-19 | 1999-08-18 | PLANT EXPRESSION VECTORS |
EP99942392A EP1104481B1 (en) | 1998-08-19 | 1999-08-18 | Plant expression vectors |
AU55783/99A AU767146B2 (en) | 1998-08-19 | 1999-08-18 | Plant expression vectors |
ARP100100133A AR075146A2 (en) | 1998-08-19 | 2010-01-21 | MOLECULA OF DNA TRANSFORMED CELL; PLANT AND METHOD TO PROVIDE A GENETIC EXPRESSION IN INCREASED PLANTS |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US9715098P | 1998-08-19 | 1998-08-19 | |
US60/097,150 | 1998-08-19 |
Publications (2)
Publication Number | Publication Date |
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WO2000011200A2 true WO2000011200A2 (en) | 2000-03-02 |
WO2000011200A3 WO2000011200A3 (en) | 2000-08-31 |
Family
ID=22261516
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
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PCT/US1999/019102 WO2000011200A2 (en) | 1998-08-19 | 1999-08-18 | Plant expression vectors |
PCT/US1999/018866 WO2000011178A2 (en) | 1998-08-19 | 1999-08-18 | An antifungal protein from tall fescue and its use in plant disease control |
PCT/US1999/018883 WO2000011185A2 (en) | 1998-08-19 | 1999-08-19 | Improved expression of cry3b insecticidal protein in plants |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1999/018866 WO2000011178A2 (en) | 1998-08-19 | 1999-08-18 | An antifungal protein from tall fescue and its use in plant disease control |
PCT/US1999/018883 WO2000011185A2 (en) | 1998-08-19 | 1999-08-19 | Improved expression of cry3b insecticidal protein in plants |
Country Status (17)
Country | Link |
---|---|
EP (5) | EP2080805B1 (en) |
CN (1) | CN100390284C (en) |
AR (2) | AR021465A1 (en) |
AT (3) | ATE506441T1 (en) |
AU (3) | AU5571699A (en) |
BR (2) | BRPI9913690B1 (en) |
CA (2) | CA2340286C (en) |
CZ (2) | CZ302074B6 (en) |
DE (3) | DE69943375D1 (en) |
DK (2) | DK1698699T3 (en) |
ES (3) | ES2365204T3 (en) |
ME (2) | ME00085B (en) |
PL (1) | PL346650A1 (en) |
PT (2) | PT1121440E (en) |
WO (3) | WO2000011200A2 (en) |
YU (1) | YU13501A (en) |
ZA (1) | ZA200100874B (en) |
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Family Cites Families (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0154204B1 (en) | 1984-03-06 | 1994-01-12 | Mgi Pharma, Inc. | Herbicide resistance in plants |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
US5004863B2 (en) | 1986-12-03 | 2000-10-17 | Agracetus | Genetic engineering of cotton plants and lines |
US5416011A (en) | 1988-07-22 | 1995-05-16 | Monsanto Company | Method for soybean transformation and regeneration |
US5639952A (en) * | 1989-01-05 | 1997-06-17 | Mycogen Plant Science, Inc. | Dark and light regulated chlorophyll A/B binding protein promoter-regulatory system |
AU638438B2 (en) * | 1989-02-24 | 1993-07-01 | Monsanto Technology Llc | Synthetic plant genes and method for preparation |
US5571697A (en) * | 1989-05-05 | 1996-11-05 | Baylor College Of Medicine Texas Medical Center | Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus |
US6127532A (en) * | 1989-09-12 | 2000-10-03 | Board Of Trustees Operating Michigan State University | Lectin cDNA and transgenic plants derived therefrom |
GB8922007D0 (en) * | 1989-09-29 | 1989-11-15 | Cambridge Advanced Tech | Light-activatable plant promoter |
US5187091A (en) * | 1990-03-20 | 1993-02-16 | Ecogen Inc. | Bacillus thuringiensis cryiiic gene encoding toxic to coleopteran insects |
US5264364A (en) * | 1991-01-31 | 1993-11-23 | Ecogen Inc. | Bacillus thuringiensis cryIIIc(B) toxin gene and protein toxic to coleopteran insects |
US5518908A (en) | 1991-09-23 | 1996-05-21 | Monsanto Company | Method of controlling insects |
US5593874A (en) | 1992-03-19 | 1997-01-14 | Monsanto Company | Enhanced expression in plants |
BR9307429A (en) * | 1992-11-12 | 1999-08-31 | Zeneca Ltd | Antimicrobial protein, composition, recombinant DNA sequence, sequence promoter, vector, biological system, plant, seeds and progeny, fungi or bacteria fighting process and extraction process for the production of a protein |
US6686200B1 (en) * | 1993-08-31 | 2004-02-03 | Uab Research Foundation | Methods and compositions for the large scale production of recombinant adeno-associated virus |
US5362865A (en) * | 1993-09-02 | 1994-11-08 | Monsanto Company | Enhanced expression in plants using non-translated leader sequences |
FR2712302B1 (en) * | 1993-11-10 | 1996-01-05 | Rhone Poulenc Agrochimie | Promoter elements of alpha tubulin chimeric genes. |
US5659123A (en) | 1994-08-26 | 1997-08-19 | Plant Genetic Systems, N.V. | Diabrotica toxins |
US5631152A (en) | 1994-10-26 | 1997-05-20 | Monsanto Company | Rapid and efficient regeneration of transgenic plants |
CA2234656C (en) * | 1995-10-13 | 2008-01-08 | Dow Agrosciences Llc | Modified bacillus thuringiensis gene for lepidopteran control in plants |
ZA974176B (en) | 1996-05-16 | 1998-09-01 | Univ North Texas | A rapid in-vitro regeneration scheme of cotton (gossypium hirsutum l.) plants compatible with agrobacterium-mediated transformation |
US5942664A (en) * | 1996-11-27 | 1999-08-24 | Ecogen, Inc. | Bacillus thuringiensis Cry1C compositions toxic to lepidopteran insects and methods for making Cry1C mutants |
EP1040192B1 (en) | 1997-12-18 | 2006-08-09 | Monsanto Technology LLC | Insect-resistant transgenic plants and methods for improving delta-endotoxin activity against insects |
US6060594A (en) | 1997-12-18 | 2000-05-09 | Ecogen, Inc. | Nucleic acid segments encoding modified bacillus thuringiensis coleopteran-toxic crystal proteins |
US6023013A (en) | 1997-12-18 | 2000-02-08 | Monsanto Company | Insect-resistant transgenic plants |
US6077824A (en) | 1997-12-18 | 2000-06-20 | Ecogen, Inc. | Methods for improving the activity of δ-endotoxins against insect pests |
US6063597A (en) | 1997-12-18 | 2000-05-16 | Monsanto Company | Polypeptide compositions toxic to coleopteran insects |
US9715098B2 (en) | 2012-05-31 | 2017-07-25 | The Board Of Trustees Of The University Of Illinois | Sparse deconvolution spatial light microscopy in two and three dimensions |
-
1999
- 1999-08-18 WO PCT/US1999/019102 patent/WO2000011200A2/en active Application Filing
- 1999-08-18 AU AU55716/99A patent/AU5571699A/en not_active Abandoned
- 1999-08-18 CZ CZ20010597A patent/CZ302074B6/en not_active IP Right Cessation
- 1999-08-18 EP EP09156877.4A patent/EP2080805B1/en not_active Expired - Lifetime
- 1999-08-18 WO PCT/US1999/018866 patent/WO2000011178A2/en active Application Filing
- 1999-08-18 DE DE69943375T patent/DE69943375D1/en not_active Expired - Lifetime
- 1999-08-18 PL PL99346650A patent/PL346650A1/en not_active IP Right Cessation
- 1999-08-18 AU AU55783/99A patent/AU767146B2/en not_active Expired
- 1999-08-18 ES ES99942392T patent/ES2365204T3/en not_active Expired - Lifetime
- 1999-08-18 EP EP14181325.3A patent/EP2811024B1/en not_active Expired - Lifetime
- 1999-08-18 CA CA2340286A patent/CA2340286C/en not_active Expired - Lifetime
- 1999-08-18 BR BRPI9913690A patent/BRPI9913690B1/en active IP Right Grant
- 1999-08-18 EP EP99942392A patent/EP1104481B1/en not_active Expired - Lifetime
- 1999-08-18 CZ CZ20090604A patent/CZ301915B6/en not_active IP Right Cessation
- 1999-08-18 AT AT99942392T patent/ATE506441T1/en not_active IP Right Cessation
- 1999-08-19 DE DE69937459T patent/DE69937459T2/en not_active Expired - Lifetime
- 1999-08-19 ES ES99942313T patent/ES2294857T3/en not_active Expired - Lifetime
- 1999-08-19 AT AT06113589T patent/ATE459717T1/en active
- 1999-08-19 BR BRPI9913089A patent/BRPI9913089B1/en active IP Right Grant
- 1999-08-19 ME MEP-2008-199A patent/ME00085B/en unknown
- 1999-08-19 PT PT99942313T patent/PT1121440E/en unknown
- 1999-08-19 AR ARP990104171A patent/AR021465A1/en not_active Application Discontinuation
- 1999-08-19 EP EP99942313A patent/EP1121440B1/en not_active Expired - Lifetime
- 1999-08-19 PT PT06113589T patent/PT1698699E/en unknown
- 1999-08-19 CA CA2340324A patent/CA2340324C/en not_active Expired - Fee Related
- 1999-08-19 ME MEP-199/08A patent/MEP19908A/en unknown
- 1999-08-19 DE DE69942109T patent/DE69942109D1/en not_active Expired - Lifetime
- 1999-08-19 DK DK06113589.3T patent/DK1698699T3/en active
- 1999-08-19 EP EP06113589A patent/EP1698699B1/en not_active Expired - Lifetime
- 1999-08-19 DK DK99942313T patent/DK1121440T3/en active
- 1999-08-19 AU AU55719/99A patent/AU5571999A/en not_active Abandoned
- 1999-08-19 AT AT99942313T patent/ATE377080T1/en active
- 1999-08-19 ES ES06113589T patent/ES2340057T3/en not_active Expired - Lifetime
- 1999-08-19 CN CNB99812169XA patent/CN100390284C/en not_active Expired - Fee Related
- 1999-08-19 WO PCT/US1999/018883 patent/WO2000011185A2/en active IP Right Grant
- 1999-08-19 YU YU13501A patent/YU13501A/en unknown
-
2001
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