WO2000011148A3 - Cold-shock regulatory elements, constructs thereof, and methods of use - Google Patents

Cold-shock regulatory elements, constructs thereof, and methods of use Download PDF

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Publication number
WO2000011148A3
WO2000011148A3 PCT/US1999/019030 US9919030W WO0011148A3 WO 2000011148 A3 WO2000011148 A3 WO 2000011148A3 US 9919030 W US9919030 W US 9919030W WO 0011148 A3 WO0011148 A3 WO 0011148A3
Authority
WO
WIPO (PCT)
Prior art keywords
cold
shock
methods
regulatory elements
inducible genes
Prior art date
Application number
PCT/US1999/019030
Other languages
French (fr)
Other versions
WO2000011148A9 (en
WO2000011148A2 (en
Inventor
Li Fang
Weining Jiang
Masanori Mitta
Masayori Inouye
Jean-Pierre Etchegaray
Kunitoshi Yamanaka
Original Assignee
Univ New Jersey Med
Li Fang
Weining Jiang
Masanori Mitta
Masayori Inouye
Etchegaray Jean Pierre
Kunitoshi Yamanaka
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/293,427 external-priority patent/US6686174B1/en
Application filed by Univ New Jersey Med, Li Fang, Weining Jiang, Masanori Mitta, Masayori Inouye, Etchegaray Jean Pierre, Kunitoshi Yamanaka filed Critical Univ New Jersey Med
Priority to EP99941243A priority Critical patent/EP1053310A1/en
Priority to AU54935/99A priority patent/AU5493599A/en
Publication of WO2000011148A2 publication Critical patent/WO2000011148A2/en
Priority to US09/552,272 priority patent/US7429654B1/en
Publication of WO2000011148A9 publication Critical patent/WO2000011148A9/en
Publication of WO2000011148A3 publication Critical patent/WO2000011148A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Regulatory elements of the 5'UTR of cold-shock inducible genes are disclosed. The elements prolong the expression of cold-shock inducible genes under conditions that elicit the cold-shock response in a bacterium, repress the expression of the cold-shock inducible genes under physiological conditions, or enhance the translation of cold-shock inducible genes under conditions that elicit a cold-shock response in bacteria. Vectors incorporating these elements are also disclosed as well as methods for expressing proteins.
PCT/US1999/019030 1994-03-01 1999-08-20 Cold-shock regulatory elements, constructs thereof, and methods of use WO2000011148A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP99941243A EP1053310A1 (en) 1998-08-20 1999-08-20 Cold-shock regulatory elements, constructs thereof, and methods of use
AU54935/99A AU5493599A (en) 1998-08-20 1999-08-20 Cold-shock regulatory elements, constructs thereof, and methods of use
US09/552,272 US7429654B1 (en) 1994-03-01 2000-04-19 Cold-shock regulatory elements, constructs thereof, and method of use

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US9693898P 1998-08-20 1998-08-20
US60/096,938 1998-08-20
US09/293,427 1999-04-16
US09/293,427 US6686174B1 (en) 1996-03-22 1999-04-16 Method and constructs for inhibiting protein expression in bacteria
US14338099P 1999-07-12 1999-07-12
US60/143,380 1999-07-12

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US09/293,427 Continuation-In-Part US6686174B1 (en) 1994-03-01 1999-04-16 Method and constructs for inhibiting protein expression in bacteria

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US09/552,272 Continuation US7429654B1 (en) 1994-03-01 2000-04-19 Cold-shock regulatory elements, constructs thereof, and method of use

Publications (3)

Publication Number Publication Date
WO2000011148A2 WO2000011148A2 (en) 2000-03-02
WO2000011148A9 WO2000011148A9 (en) 2000-06-02
WO2000011148A3 true WO2000011148A3 (en) 2000-07-13

Family

ID=27378262

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/019030 WO2000011148A2 (en) 1994-03-01 1999-08-20 Cold-shock regulatory elements, constructs thereof, and methods of use

Country Status (3)

Country Link
EP (1) EP1053310A1 (en)
AU (1) AU5493599A (en)
WO (1) WO2000011148A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW200510534A (en) * 2002-12-11 2005-03-16 Takara Bio Inc Cold-induced expression vector

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998027220A1 (en) * 1996-12-19 1998-06-25 University Of Medicine And Dentistry Of New Jersey Method and constructs for inhibiting protein expression in bacteria
WO1999027117A1 (en) * 1997-11-20 1999-06-03 Takara Shuzo Co., Ltd. Cold-inducible expression vector

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998027220A1 (en) * 1996-12-19 1998-06-25 University Of Medicine And Dentistry Of New Jersey Method and constructs for inhibiting protein expression in bacteria
WO1999027117A1 (en) * 1997-11-20 1999-06-03 Takara Shuzo Co., Ltd. Cold-inducible expression vector

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FANG ET AL.: "Transcription of cspA, the gene for the major cold-shock protein of Escherichia coli, is negatively regulated at 37 degrees C by the 5'-untranslated region of its mRNA", FEMS MICROBIOL LETT, vol. 176, no. 1, 1 July 1999 (1999-07-01), pages 39 - 43, XP002133946 *
SPRENGART ET AL.: "The downstream box: an efficient and independent translation initiation signal in Escherichia coli", THE EMBO JOURNAL, vol. 15, no. 3, 1996, pages 665 - 674, XP002133947 *

Also Published As

Publication number Publication date
AU5493599A (en) 2000-03-14
WO2000011148A9 (en) 2000-06-02
EP1053310A1 (en) 2000-11-22
WO2000011148A2 (en) 2000-03-02

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