WO1999066805A1 - Flavor enhancer - Google Patents

Flavor enhancer Download PDF

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Publication number
WO1999066805A1
WO1999066805A1 PCT/EP1999/004076 EP9904076W WO9966805A1 WO 1999066805 A1 WO1999066805 A1 WO 1999066805A1 EP 9904076 W EP9904076 W EP 9904076W WO 9966805 A1 WO9966805 A1 WO 9966805A1
Authority
WO
WIPO (PCT)
Prior art keywords
process according
aqueous medium
cheese
amount
total weight
Prior art date
Application number
PCT/EP1999/004076
Other languages
French (fr)
Other versions
WO1999066805B1 (en
Inventor
Claude Lecouteux
Hugues Guichard
Original Assignee
Societe Des Produits Nestle S.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Societe Des Produits Nestle S.A. filed Critical Societe Des Produits Nestle S.A.
Priority to EP99927969A priority Critical patent/EP1089636B1/en
Priority to AU45126/99A priority patent/AU763511B2/en
Priority to AT99927969T priority patent/ATE232049T1/en
Priority to DE69905250T priority patent/DE69905250T2/en
Priority to CA002333139A priority patent/CA2333139A1/en
Publication of WO1999066805A1 publication Critical patent/WO1999066805A1/en
Publication of WO1999066805B1 publication Critical patent/WO1999066805B1/en
Priority to HK01106183A priority patent/HK1035302A1/en

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Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C19/00—Cheese; Cheese preparations; Making thereof
    • A23C19/06—Treating cheese curd after whey separation; Products obtained thereby
    • A23C19/09—Other cheese preparations; Mixtures of cheese with other foodstuffs
    • A23C19/0904—Liquid cheese products, e.g. beverages, sauces
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20—Synthetic spices, flavouring agents or condiments
    • A23L27/24—Synthetic spices, flavouring agents or condiments prepared by fermentation
    • A23L27/25—Dairy flavours

Definitions

  • the present invention relates to a flavor enhancer and more particularly to a natural cheese flavor enhancer which boosts the cheese flavor in cheese containing products.
  • natural cheese flavors are made from starting materials such as aged cheese, cheese block or cheese curds which are expensive. Also, it is well known that cheese based raw materials are of variable and unreliable quality. Such starting materials are usually treated with enzymes such as proteases and it is well known that enzyme modified cheese products have an unpleasant bitter flavor. In addition, in some cases, incubation with microorganisms such as Penicillium roqueforti or lactic acid bacteria is carried out for up to 48 hours or more which increases the cost and makes the process rather complicated and time-consuming.
  • starting materials such as aged cheese, cheese block or cheese curds which are expensive.
  • enzymes such as proteases
  • enzyme modified cheese products have an unpleasant bitter flavor.
  • incubation with microorganisms such as Penicillium roqueforti or lactic acid bacteria is carried out for up to 48 hours or more which increases the cost and makes the process rather complicated and time-consuming.
  • the present invention provides a process for the preparation of a natural cheese flavor enhancer which comprises incubating a protein, a fat, a protease and a lipase in an aqueous medium under conditions suitable for producing a cheese flavor enhancer.
  • the cheese flavor enhancer is not a cheese flavor in itself: if it is added to a bland product, it does not impart any specific flavor. On the other hand, when the cheese flavor enhancer is added to a cheese-containing product, it enhances the cheese flavor enabling the amount of expensive cheese normally present in the product to be reduced substantially.
  • Any edible protein or fat may be used in the process of the invention.
  • Preferred examples of protein are whey protein concentrate, lactalbumin or casein, preferably rennet casein, sodium or calcium caseinate.
  • the fat may be an animal or vegetable fat but preferred examples of fats are corn oil, shortening ® (partially hydrogenated soybean and cottonseed oils, mono- and diglycerides), heavy cream or butter. If desired, mixtures of proteins and/or mixtures of fats may be used.
  • the amount of protein used may be from 1 to 30%, preferably from 5 to 25% and more preferably from 10 to 16% by weight based on the total weight of the aqueous medium.
  • the amount of fat may be from 5 to 50%, preferably from 10 to 40% and more preferably from 20 to 35% by weight based on the total weight of the aqueous medium.
  • whey protein may be added to the aqueous medium, e.g. from 1 to 10% and preferably from 2 to 6% by weight based on the total weight of the aqueous medium.
  • Whey protein is used as a filler and allows to increase the solid content of the cheese enhancer with a limited impact on the flavor and the cost.
  • protease or lipase may be used in the process of the invention.
  • the protease may be Protease 2A manufactured by Amano Enzyme Co., a neutral protease, e.g. Protease P Amano 6 manufactured by the Amano Enzyme Co., or Neutrase or Flavourzyme manufactured by Novo Nordisk, Inc.
  • a mixture of one or more proteases may be used if desired.
  • the amount of protease used may be from 0.05 to 1.0% and preferably from 0.1 to 0.75% by weight based on the total weight of the aqueous medium.
  • the lipase may be pancreatic lipase manufactured by Valley Research Inc., pregastric esterase manufactured by Amano Enzyme Co., or a fungal lipase such as Palatase 20,000L manufactured by Novo Nordisk Inc. or any mixture thereof.
  • the amount of lipase used may be from 0.05 to 1.0% and preferably from 0.1 to 0.75% by weight based on the total weight of the aqueous medium.
  • the amount of water in the aqueous medium may be from 25 to 75%, preferably from 35 to 65% and more preferably from 40 to 60% by weight based on the total weight of the aqueous medium.
  • Salt may be added, if desired, e.g. in an amount of from 0.5 to 5%, preferably from 1 to 3.5% by weight based on the total weight of the aqueous medium.
  • Other ingredients conventionally used in cheese making may also be added to the aqueous medium, e.g. trisodium phosphate in an amount up to 2% and preferably from 0.5 to 1.5%, a fungicide such as sorbic acid or potassium sorbate in an amount up to 0.5% and preferably from 0.05 to 0.25% by weight, and a thickener or stabilizer such as xanthan gum, guar gum or carrageenan, etc. in an amount up to 0.50% and preferably from 0.02 to 0.2% all amounts being by weight based on the total weight of the aqueous medium.
  • the incubation is conveniently carried out in a reaction vessel, such as an incubator.
  • the ingredients are loaded into the reaction vessel, preferably in the following order: the water is introduced first followed by the protease, then the proteins, then the lipase and finally the fats.
  • the trisodium phosphate and the salt are added with the water while the fungicides, thickeners and/or stabilizers are preferably added after the addition of the proteins but before the addition of the fats.
  • the temperature of the incubation may be from 37° to 48°C (100° to 117°F) and preferably from 39° to 46°C (104° to 113°F).
  • the pH of the incubation may be from 4 to 6, preferably from 4.5 to 5.5.
  • the duration of the incubation may be from 12 to 48 hours, preferably from 18 to 36 hours.
  • the incubation is carried out in the absence of any added microorganism.
  • the pH may be adjusted to 4.2-4.4, preferably with phosphoric acid.
  • the product is advantageously homogenized and then pasteurized, e.g. at a temperature from 90°C to 100°C for a period of from 2.5 to 10 minutes, preferably at a temperature from 94°C to 99°C for a period of from 4 to 8 minutes.
  • the product is conveniently cooled, e.g. to a temperature of from 4° to 10°C to give a product having the consistency of a paste.
  • the product may be used as a paste or dried and used as a powder. The drying is preferably achieved by spray drying, using a carrier, for instance, maltodextrin in an amount of from 20 to 40% by weight based on the weight of the paste.
  • the moisture content of the final powder is usually from about 2.0 to 4.0% by weight.
  • the natural cheese flavor enhancer produced by the process of the present invention may be used to enhance the flavor of cheese-containing products such as cheese sauces, cheese dips and salad dressing.
  • the present invention therefore also provides a cheese-containing product containing an effective amount of the natural cheese flavor enhancer obtainable by the process of the present invention.
  • the amount of the natural cheese flavor enhancer added to the cheese-containing product may be from 0.1 to 5%, preferably from 0.25 to 2.5% and more preferably from 0.5 to 1.5% by weight by weight based on the total weight of the cheese- containing product.
  • the product is then piston homogenized at 500 psi.
  • the homogenized mixture is pumped to a plate heat exchanger and pasteurized at 95°C (203°F) for 5 minutes in a holding tube, cooled to 4.5°C (40°F) and transported to a filler where it is packaged and stored at 5°C (41°F).
  • the cheese content of a cheese sauce normally containing 33% of cheese is reduced to 25%.
  • To 100 parts of the cheese sauce containing 25% of cheese is added 1 part of the natural cheese flavor enhancer prepared in Example 1.
  • the cheese flavor of the cheese sauce containing the natural cheese flavor enhancer is as good as that of the cheese sauce containing 33% of cheese.

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Dairy Products (AREA)
  • Seasonings (AREA)

Abstract

A process for the preparation of a natural cheese flavor enhancer which comprises incubating a protein, a fat, a protease and lipase in an aqueous medium under conditions suitable for producing a cheese flavor enhancer.

Description

FLAVOR ENHANCER
The present invention relates to a flavor enhancer and more particularly to a natural cheese flavor enhancer which boosts the cheese flavor in cheese containing products.
Currently, natural cheese flavors are made from starting materials such as aged cheese, cheese block or cheese curds which are expensive. Also, it is well known that cheese based raw materials are of variable and unreliable quality. Such starting materials are usually treated with enzymes such as proteases and it is well known that enzyme modified cheese products have an unpleasant bitter flavor. In addition, in some cases, incubation with microorganisms such as Penicillium roqueforti or lactic acid bacteria is carried out for up to 48 hours or more which increases the cost and makes the process rather complicated and time-consuming.
We have now devised a process for the preparation of a natural cheese flavor enhancer with reduced bitterness compared with other enzyme modified cheese products and which eliminates the need for the employment of expensive raw materials. In our process, the starting materials are proteins and fats rather than the more expensive aged cheese, cheese block or cheese curds and, in addition microorganisms are not used.
Accordingly, the present invention provides a process for the preparation of a natural cheese flavor enhancer which comprises incubating a protein, a fat, a protease and a lipase in an aqueous medium under conditions suitable for producing a cheese flavor enhancer.
It should be understood that in this invention, the cheese flavor enhancer is not a cheese flavor in itself: if it is added to a bland product, it does not impart any specific flavor. On the other hand, when the cheese flavor enhancer is added to a cheese-containing product, it enhances the cheese flavor enabling the amount of expensive cheese normally present in the product to be reduced substantially.
Any edible protein or fat may be used in the process of the invention. Preferred examples of protein are whey protein concentrate, lactalbumin or casein, preferably rennet casein, sodium or calcium caseinate. The fat may be an animal or vegetable fat but preferred examples of fats are corn oil, shortening® (partially hydrogenated soybean and cottonseed oils, mono- and diglycerides), heavy cream or butter. If desired, mixtures of proteins and/or mixtures of fats may be used.
The amount of protein used may be from 1 to 30%, preferably from 5 to 25% and more preferably from 10 to 16% by weight based on the total weight of the aqueous medium. The amount of fat may be from 5 to 50%, preferably from 10 to 40% and more preferably from 20 to 35% by weight based on the total weight of the aqueous medium.
If desired, a small amount of whey protein may be added to the aqueous medium, e.g. from 1 to 10% and preferably from 2 to 6% by weight based on the total weight of the aqueous medium. Whey protein is used as a filler and allows to increase the solid content of the cheese enhancer with a limited impact on the flavor and the cost.
Any protease or lipase may be used in the process of the invention. For example, the protease may be Protease 2A manufactured by Amano Enzyme Co., a neutral protease, e.g. Protease P Amano 6 manufactured by the Amano Enzyme Co., or Neutrase or Flavourzyme manufactured by Novo Nordisk, Inc. A mixture of one or more proteases may be used if desired. The amount of protease used may be from 0.05 to 1.0% and preferably from 0.1 to 0.75% by weight based on the total weight of the aqueous medium.
The lipase may be pancreatic lipase manufactured by Valley Research Inc., pregastric esterase manufactured by Amano Enzyme Co., or a fungal lipase such as Palatase 20,000L manufactured by Novo Nordisk Inc. or any mixture thereof. The amount of lipase used may be from 0.05 to 1.0% and preferably from 0.1 to 0.75% by weight based on the total weight of the aqueous medium.
The amount of water in the aqueous medium may be from 25 to 75%, preferably from 35 to 65% and more preferably from 40 to 60% by weight based on the total weight of the aqueous medium.
Salt may be added, if desired, e.g. in an amount of from 0.5 to 5%, preferably from 1 to 3.5% by weight based on the total weight of the aqueous medium. Other ingredients conventionally used in cheese making may also be added to the aqueous medium, e.g. trisodium phosphate in an amount up to 2% and preferably from 0.5 to 1.5%, a fungicide such as sorbic acid or potassium sorbate in an amount up to 0.5% and preferably from 0.05 to 0.25% by weight, and a thickener or stabilizer such as xanthan gum, guar gum or carrageenan, etc. in an amount up to 0.50% and preferably from 0.02 to 0.2% all amounts being by weight based on the total weight of the aqueous medium.
The incubation is conveniently carried out in a reaction vessel, such as an incubator. The ingredients are loaded into the reaction vessel, preferably in the following order: the water is introduced first followed by the protease, then the proteins, then the lipase and finally the fats. Advantageously, the trisodium phosphate and the salt are added with the water while the fungicides, thickeners and/or stabilizers are preferably added after the addition of the proteins but before the addition of the fats.
The temperature of the incubation may be from 37° to 48°C (100° to 117°F) and preferably from 39° to 46°C (104° to 113°F).
The pH of the incubation may be from 4 to 6, preferably from 4.5 to 5.5.
The duration of the incubation may be from 12 to 48 hours, preferably from 18 to 36 hours. The incubation is carried out in the absence of any added microorganism.
After incubation, the pH may be adjusted to 4.2-4.4, preferably with phosphoric acid.
After incubation, the product is advantageously homogenized and then pasteurized, e.g. at a temperature from 90°C to 100°C for a period of from 2.5 to 10 minutes, preferably at a temperature from 94°C to 99°C for a period of from 4 to 8 minutes. After pasteurization, the product is conveniently cooled, e.g. to a temperature of from 4° to 10°C to give a product having the consistency of a paste. The product may be used as a paste or dried and used as a powder. The drying is preferably achieved by spray drying, using a carrier, for instance, maltodextrin in an amount of from 20 to 40% by weight based on the weight of the paste. The moisture content of the final powder is usually from about 2.0 to 4.0% by weight.
The natural cheese flavor enhancer produced by the process of the present invention may be used to enhance the flavor of cheese-containing products such as cheese sauces, cheese dips and salad dressing.
The present invention therefore also provides a cheese-containing product containing an effective amount of the natural cheese flavor enhancer obtainable by the process of the present invention.
The amount of the natural cheese flavor enhancer added to the cheese-containing product may be from 0.1 to 5%, preferably from 0.25 to 2.5% and more preferably from 0.5 to 1.5% by weight by weight based on the total weight of the cheese- containing product.
EXAMPLES
The following Examples further illustrate the present invention. Parts and percentages are given by weight except where stated.
Example 1
50 parts of water are mixed with 0.35 parts of Protease 2A, 2.229 parts of NaCl and then 1 part of trisodium phosphate and loaded into a 500 gallon liquefier. This is followed by in order of addition: 4.244 parts of sweet whey, 13.5 parts of rennet casein (30 mesh), 0.1 part of potassium sorbate, 0.05 parts of xanthan gum (Keltrol RD), 0.35 parts of pancreatic lipase and 28.177 parts of butter. The ingredients are ground and then pumped to a 300 gallon reaction tank jacketed with water at 45°C (113°F), and incubated for 24 hours. After incubation, the pH is adjusted to 4.2-4.4 with phosphoric acid. The product is then piston homogenized at 500 psi. The homogenized mixture is pumped to a plate heat exchanger and pasteurized at 95°C (203°F) for 5 minutes in a holding tube, cooled to 4.5°C (40°F) and transported to a filler where it is packaged and stored at 5°C (41°F).
Example 2
The cheese content of a cheese sauce normally containing 33% of cheese is reduced to 25%. To 100 parts of the cheese sauce containing 25% of cheese is added 1 part of the natural cheese flavor enhancer prepared in Example 1. The cheese flavor of the cheese sauce containing the natural cheese flavor enhancer is as good as that of the cheese sauce containing 33% of cheese.

Claims

1. A process for the preparation of a natural cheese flavor enhancer which comprises incubating a protein, a fat, a protease and a lipase in an aqueous medium under conditions suitable for producing a cheese flavor enhancer.
2. A process according to claim 1 wherein the protein used is a casein, a whey protein concentrate or lactalbumin.
3. A process according to claim 1 or claim 2 wherein the fat used is a vegetable oil.
4. A process according to any of claims 1 to 3 wherein the fat used is corn oil, heavy cream or butter.
5. A process according to any of claims 1 to 4 wherein a mixture of proteins and/or of fats is used.
6. A process according to any of claims 1 to 5 wherein the amount of protein used is from 1 to 30% by weight based on the total weight of the aqueous medium.
7. A process according to any of claims 1 to 6 wherein the amount of fat is from 5 to 50% by weight based on the total weight of the aqueous medium.
8. A process according to any of claims 1 to 7 wherein from 1 to 10% of whey protein is added to the aqueous medium by weight based on the total weight of the aqueous medium.
9. A process according to any of claims 1 to 8 wherein the amount of protease used is from 0.05 to 1.0% by weight based on the total weight of the aqueous medium.
10. A process according to any of claims 1 to 9 wherein the amount of lipase used is from 0.05 to 1.0% by weight based on the total weight of the aqueous medium.
11. A process according to any of claims 1 to 10 wherein the amount of water in the aqueous medium is from 25 to 75% by weight based on the total weight of the aqueous medium.
12. A process according to any of claims 1 to 11 wherein the ingredients are loaded into the reaction vessel wherein the water is introduced first followed by the protease, then the proteins, then the lipase and finally the fats.
13. A process according to any of claims 1 to 12 wherein the temperature of the incubation is from 37┬░ to 48┬░C.
14. A process according to any of claims 1 to 13 wherein the pH of the incubation is from 4 to 6.
15. A process according to any of claims 1 to 14 wherein the duration of the incubation is from 12 to 48 hours.
16. A process according to any of claims 1 to 15 wherein, after incubation, the product is homogenized and then pasteurized before cooling to a temperature of from 4┬░ to lO┬░C.
17. A process according to any of claims 1 to 16 wherein the cheese flavor enhancer is made into a paste or powder.
18. A process according to any of claims 1 to 17 wherein the moisture content of the final powder is from about 2.0 to 4.0% by weight.
19. A cheese-containing product containing an effective amount of a natural cheese flavor enhancer obtainable by the process of any of the preceding claims.
20. A cheese-containing product according to claim 19 wherein the amount of the natural cheese flavor enhancer is from 0.1 to 5% by weight by weight based on the total weight of the cheese-containing product.
PCT/EP1999/004076 1998-06-23 1999-06-14 Flavor enhancer WO1999066805A1 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP99927969A EP1089636B1 (en) 1998-06-23 1999-06-14 Flavor enhancer
AU45126/99A AU763511B2 (en) 1998-06-23 1999-06-14 Flavor enhancer
AT99927969T ATE232049T1 (en) 1998-06-23 1999-06-14 FLAVOR ENHANCER
DE69905250T DE69905250T2 (en) 1998-06-23 1999-06-14 TASTE AMPLIFIERS
CA002333139A CA2333139A1 (en) 1998-06-23 1999-06-14 Flavor enhancer
HK01106183A HK1035302A1 (en) 1998-06-23 2001-08-31 Flavor enhancer

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/103,248 1998-06-23
US09/103,248 US6022567A (en) 1998-06-23 1998-06-23 Flavor enhancer

Publications (2)

Publication Number Publication Date
WO1999066805A1 true WO1999066805A1 (en) 1999-12-29
WO1999066805B1 WO1999066805B1 (en) 2000-03-02

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP1999/004076 WO1999066805A1 (en) 1998-06-23 1999-06-14 Flavor enhancer

Country Status (12)

Country Link
US (1) US6022567A (en)
EP (1) EP1089636B1 (en)
AR (1) AR018907A1 (en)
AT (1) ATE232049T1 (en)
AU (1) AU763511B2 (en)
CA (1) CA2333139A1 (en)
CO (1) CO5070611A1 (en)
DE (1) DE69905250T2 (en)
ES (1) ES2190223T3 (en)
HK (1) HK1035302A1 (en)
MY (1) MY116030A (en)
WO (1) WO1999066805A1 (en)

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US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
WO2008036863A2 (en) 2006-09-21 2008-03-27 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
WO2010025395A2 (en) 2008-08-29 2010-03-04 Verenium Corporation Hydrolases, nucleic acids encoding them and methods for making and using them
EP2216403A2 (en) 2006-02-02 2010-08-11 Verenium Corporation Esterases and related nucleic acids and methods
WO2011046815A1 (en) 2009-10-16 2011-04-21 Bunge Oils, Inc. Oil degumming methods
WO2011046812A1 (en) 2009-10-16 2011-04-21 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7943360B2 (en) 2002-04-19 2011-05-17 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US8298799B2 (en) 2003-03-07 2012-10-30 Dsm Ip Assets B. V. Hydrolases, nucleic acids encoding them and methods for making and using them
US8557551B2 (en) 2004-09-10 2013-10-15 Dsm Ip Assets B.V. Compositions and methods for making and modifying oils
US9017990B2 (en) 2004-09-10 2015-04-28 Dsm Ip Assets B.V. Methods for enzymatic decolorization of chlorophyll
WO2018028081A1 (en) * 2016-08-11 2018-02-15 广州市名花香料有限公司 Cheese powder flavor and preparation method therefor

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US6787168B1 (en) * 2003-03-18 2004-09-07 James W. Sawhill Peptide product
US7364766B2 (en) * 2003-10-10 2008-04-29 Frito-Lay North America, Inc. Toasted flavor additive and method of making
US7556833B2 (en) * 2003-11-26 2009-07-07 Kraft Foods Global Brands Llc Cheese flavoring systems prepared with bacteriocins
US7776370B2 (en) * 2005-09-01 2010-08-17 Kraft Foods Global Brands Llc Heat-stable flavoring components and cheese flavoring systems incorporating them
US8703217B2 (en) * 2006-03-31 2014-04-22 Kraft Foods Group Brands Llc Methods for rapid production and usage of biogenerated flavors
CN102742816A (en) * 2011-04-21 2012-10-24 上海爱普植物科技有限公司 Method for preparation of natural cheese essence with whey powder
CN103053698A (en) * 2013-01-18 2013-04-24 中国农业科学院农产品加工研究所 Enzymatic modified cheese powder and preparation method thereof
CN103315072B (en) * 2013-06-03 2014-07-16 华南理工大学 Preparation method for non-dairy cream stabilized by solid particles

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US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7943360B2 (en) 2002-04-19 2011-05-17 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US7977080B2 (en) 2002-04-19 2011-07-12 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
US8298799B2 (en) 2003-03-07 2012-10-30 Dsm Ip Assets B. V. Hydrolases, nucleic acids encoding them and methods for making and using them
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US9193962B2 (en) 2003-03-07 2015-11-24 Dsm Ip Assets B.V. Hydrolases, nucleic acids encoding them and methods for making and using them
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AU4512699A (en) 2000-01-10
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DE69905250T2 (en) 2003-10-30
ATE232049T1 (en) 2003-02-15
WO1999066805B1 (en) 2000-03-02
CA2333139A1 (en) 1999-12-29
EP1089636A1 (en) 2001-04-11
CO5070611A1 (en) 2001-08-28
DE69905250D1 (en) 2003-03-13
AU763511B2 (en) 2003-07-24
US6022567A (en) 2000-02-08
AR018907A1 (en) 2001-12-12
EP1089636B1 (en) 2003-02-05
HK1035302A1 (en) 2001-11-23

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