WO1999065938A3 - Fy7 polymerase - Google Patents
Fy7 polymerase Download PDFInfo
- Publication number
- WO1999065938A3 WO1999065938A3 PCT/US1999/013741 US9913741W WO9965938A3 WO 1999065938 A3 WO1999065938 A3 WO 1999065938A3 US 9913741 W US9913741 W US 9913741W WO 9965938 A3 WO9965938 A3 WO 9965938A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- dna
- dna polymerase
- activity
- nucleic acid
- recombinant
- Prior art date
Links
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 3
- 108020004414 DNA Proteins 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 150000003839 salts Chemical class 0.000 abstract 2
- 238000012163 sequencing technique Methods 0.000 abstract 2
- 108020004511 Recombinant DNA Proteins 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
Abstract
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2330735A CA2330735C (en) | 1998-06-17 | 1999-06-17 | Fy7 polymerase |
JP2000554763A JP4349545B2 (en) | 1998-06-17 | 1999-06-17 | FY7 polymerase |
DE69936919T DE69936919T2 (en) | 1998-06-17 | 1999-06-17 | FY7 POLYMERASE |
AU45757/99A AU768993B2 (en) | 1998-06-17 | 1999-06-17 | FY7 polymerase |
EP99928766A EP1086209B1 (en) | 1998-06-17 | 1999-06-17 | Fy7 polymerase |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US8955698P | 1998-06-17 | 1998-06-17 | |
US60/089,556 | 1998-06-17 |
Publications (3)
Publication Number | Publication Date |
---|---|
WO1999065938A2 WO1999065938A2 (en) | 1999-12-23 |
WO1999065938A3 true WO1999065938A3 (en) | 2000-04-06 |
WO1999065938A9 WO1999065938A9 (en) | 2000-06-08 |
Family
ID=22218300
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1999/013741 WO1999065938A2 (en) | 1998-06-17 | 1999-06-17 | Fy7 polymerase |
Country Status (9)
Country | Link |
---|---|
US (1) | US6479267B1 (en) |
EP (1) | EP1086209B1 (en) |
JP (1) | JP4349545B2 (en) |
CN (1) | CN1134538C (en) |
AT (1) | ATE371022T1 (en) |
AU (1) | AU768993B2 (en) |
CA (1) | CA2330735C (en) |
DE (1) | DE69936919T2 (en) |
WO (1) | WO1999065938A2 (en) |
Families Citing this family (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7875440B2 (en) | 1998-05-01 | 2011-01-25 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
AU2002316586A1 (en) * | 2001-07-06 | 2003-01-21 | Amersham Biosciences Corp | Novel dna polymerases having amino acid substitutions and homologs thereof |
WO2003040395A2 (en) * | 2001-11-07 | 2003-05-15 | Applera Corporation | Universal nucleotides for nucleic acid analysis |
US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
US7981604B2 (en) | 2004-02-19 | 2011-07-19 | California Institute Of Technology | Methods and kits for analyzing polynucleotide sequences |
AU2005254984B2 (en) * | 2004-06-10 | 2008-10-16 | Ge Healthcare Bio-Sciences Corp. | Method for nucleic acid analysis |
BRPI0515777A (en) * | 2004-12-11 | 2008-08-05 | Cytogenix Inc | biosynthesis free from high quality nucleic acid cells and uses thereof |
US7666593B2 (en) | 2005-08-26 | 2010-02-23 | Helicos Biosciences Corporation | Single molecule sequencing of captured nucleic acids |
EP3447141B1 (en) | 2013-03-18 | 2020-08-05 | PreAnalytiX GmbH | Stabilisation of biological samples |
GB201721053D0 (en) * | 2017-12-15 | 2018-01-31 | Univ I Tromsø - Norges Arktiske Univ | DNA polymerases |
CN110093410A (en) * | 2019-05-21 | 2019-08-06 | 通用生物系统(安徽)有限公司 | A kind of DNA sequencing reaction reagent and preparation method thereof |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991009950A1 (en) * | 1989-12-22 | 1991-07-11 | F. Hoffmann-La Roche Ag | Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase |
EP0745676A1 (en) * | 1995-05-31 | 1996-12-04 | Amersham Life Science Inc | Thermostable DNA polymerases |
US5593840A (en) * | 1993-01-27 | 1997-01-14 | Oncor, Inc. | Amplification of nucleic acid sequences |
WO1998001562A1 (en) * | 1996-07-05 | 1998-01-15 | The Mount Sinai School Of Medicine Of The City University Of New York | Thermostable mutl genes and proteins and uses therefor |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5795762A (en) | 1986-08-22 | 1998-08-18 | Roche Molecular Systems, Inc. | 5' to 3' exonuclease mutations of thermostable DNA polymerases |
US5618711A (en) | 1986-08-22 | 1997-04-08 | Hoffmann-La Roche Inc. | Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase |
JP2531246B2 (en) * | 1988-08-26 | 1996-09-04 | 東洋紡績株式会社 | Thermostable DNA polymerase and method for producing the same |
US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
US5610066A (en) | 1993-12-10 | 1997-03-11 | Amersham Life Science, Inc. | Nucleic acid modifying proteins from Pyrococcus furiosus |
WO1995030756A1 (en) | 1994-04-18 | 1995-11-16 | United States Biochemical Corporation | Thermostable alkaline phosphatase of thermus thermophilus |
AU7236296A (en) * | 1995-09-08 | 1997-03-27 | Life Technologies, Inc. | Cloned dna polymerases from thermotoga and mutants thereof |
ATE203056T1 (en) | 1995-12-15 | 2001-07-15 | Amersham Life Science Inc | THERMOSTABLE DNA POLYMERASE FROM THERMOANAEROBACTER THERMOHYDROSULFURICUS AND MUTATED ENZYMES DERIVED THEREOF WITH REMOVED EXONUCLEASE ACTIVITY |
-
1999
- 1999-06-17 EP EP99928766A patent/EP1086209B1/en not_active Expired - Lifetime
- 1999-06-17 AU AU45757/99A patent/AU768993B2/en not_active Ceased
- 1999-06-17 US US09/334,818 patent/US6479267B1/en not_active Expired - Lifetime
- 1999-06-17 WO PCT/US1999/013741 patent/WO1999065938A2/en active IP Right Grant
- 1999-06-17 AT AT99928766T patent/ATE371022T1/en not_active IP Right Cessation
- 1999-06-17 CN CNB998098124A patent/CN1134538C/en not_active Expired - Fee Related
- 1999-06-17 JP JP2000554763A patent/JP4349545B2/en not_active Expired - Fee Related
- 1999-06-17 CA CA2330735A patent/CA2330735C/en not_active Expired - Fee Related
- 1999-06-17 DE DE69936919T patent/DE69936919T2/en not_active Expired - Lifetime
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991009950A1 (en) * | 1989-12-22 | 1991-07-11 | F. Hoffmann-La Roche Ag | Recombinant expression vectors and purification methods for thermus thermophilus dna polymerase |
US5593840A (en) * | 1993-01-27 | 1997-01-14 | Oncor, Inc. | Amplification of nucleic acid sequences |
EP0745676A1 (en) * | 1995-05-31 | 1996-12-04 | Amersham Life Science Inc | Thermostable DNA polymerases |
WO1998001562A1 (en) * | 1996-07-05 | 1998-01-15 | The Mount Sinai School Of Medicine Of The City University Of New York | Thermostable mutl genes and proteins and uses therefor |
Non-Patent Citations (2)
Title |
---|
GUTMAN P.D. ET AL: "Conserved sites in the 5'-3' exonuclease domain of Escherichia coli DNA polymerase", NUCLEIC ACIDS RESEARCH, vol. 21, no. 18, 11 September 1993 (1993-09-11), pages 4406 - 4407, XP000857830 * |
REEVE M A ET AL: "A NOVEL THERMOSTABLE POLYMERASE FOR DNA SEQUENCING", NATURE,GB,MACMILLAN JOURNALS LTD. LONDON, vol. 376, pages 796-797, XP000606193, ISSN: 0028-0836 * |
Also Published As
Publication number | Publication date |
---|---|
AU4575799A (en) | 2000-01-05 |
EP1086209A2 (en) | 2001-03-28 |
CN1313893A (en) | 2001-09-19 |
CA2330735C (en) | 2010-05-11 |
CN1134538C (en) | 2004-01-14 |
JP2002517997A (en) | 2002-06-25 |
AU768993B2 (en) | 2004-01-15 |
CA2330735A1 (en) | 1999-12-23 |
EP1086209B1 (en) | 2007-08-22 |
DE69936919D1 (en) | 2007-10-04 |
US6479267B1 (en) | 2002-11-12 |
WO1999065938A9 (en) | 2000-06-08 |
WO1999065938A2 (en) | 1999-12-23 |
ATE371022T1 (en) | 2007-09-15 |
DE69936919T2 (en) | 2008-05-15 |
JP4349545B2 (en) | 2009-10-21 |
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