WO1999054494A3 - Multiparameter facs assays to detect alterations in cellular parameters - Google Patents

Multiparameter facs assays to detect alterations in cellular parameters Download PDF

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Publication number
WO1999054494A3
WO1999054494A3 PCT/US1999/008345 US9908345W WO9954494A3 WO 1999054494 A3 WO1999054494 A3 WO 1999054494A3 US 9908345 W US9908345 W US 9908345W WO 9954494 A3 WO9954494 A3 WO 9954494A3
Authority
WO
WIPO (PCT)
Prior art keywords
cellular parameters
detect alterations
facs assays
cell
multiparameter
Prior art date
Application number
PCT/US1999/008345
Other languages
French (fr)
Other versions
WO1999054494A2 (en
Inventor
Joseph Fisher
James Lorens
Donald Payan
Alexander Rossi
Original Assignee
Rigel Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/062,330 external-priority patent/US6897031B1/en
Priority claimed from US09/157,748 external-priority patent/US6461813B2/en
Priority to DK99917563T priority Critical patent/DK1071809T3/en
Priority to CA2325597A priority patent/CA2325597C/en
Priority to CNB998070904A priority patent/CN100334227C/en
Priority to NZ508120A priority patent/NZ508120A/en
Application filed by Rigel Pharmaceuticals Inc filed Critical Rigel Pharmaceuticals Inc
Priority to AT99917563T priority patent/ATE248226T1/en
Priority to JP2000544822A priority patent/JP4511033B2/en
Priority to EP99917563A priority patent/EP1071809B1/en
Priority to AU35654/99A priority patent/AU3565499A/en
Priority to DE69910756T priority patent/DE69910756T2/en
Publication of WO1999054494A2 publication Critical patent/WO1999054494A2/en
Publication of WO1999054494A3 publication Critical patent/WO1999054494A3/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/43504—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates
    • G01N2333/43595—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/46—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans from vertebrates
    • G01N2333/47—Assays involving proteins of known structure or function as defined in the subgroups
    • G01N2333/4701—Details
    • G01N2333/4718—Lipocortins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • Cephalosporin Compounds (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Abstract

The invention relates to novel methods of detecting alterations in cell cycle regulation in a cell or a cell population and screening for agents capable of modulating cell cycle regulation through the use of multiparameter assays and a fluorescence-activated cell sorter (FACS) machine.
PCT/US1999/008345 1998-04-17 1999-04-16 Multiparameter facs assays to detect alterations in cellular parameters WO1999054494A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
DE69910756T DE69910756T2 (en) 1998-04-17 1999-04-16 MULTIPARAMETER FACS TEST FOR DETECTING CHANGES IN THE CELLULAR PARAMETERS
AU35654/99A AU3565499A (en) 1998-04-17 1999-04-16 Multiparameter facs assays to detect alterations in cellular parameters and to screen small molecule libraries
CA2325597A CA2325597C (en) 1998-04-17 1999-04-16 Multiparameter facs assays to detect alterations in cellular parameters and to screen small molecule libraries
CNB998070904A CN100334227C (en) 1998-04-17 1999-04-16 Multiparameter FACS assays to detect alterations in cellular parameters and screening
NZ508120A NZ508120A (en) 1998-04-17 1999-04-16 Multiparameter FACS assays to detect alterations in cellular parameters and to screen small molecule libraries
DK99917563T DK1071809T3 (en) 1998-04-17 1999-04-16 Multiparameter FACS test to detect changes in cellular parameters
AT99917563T ATE248226T1 (en) 1998-04-17 1999-04-16 MULTIPARAMETER FACS TEST TO DETERMINE CHANGES IN CELLULAR PARAMETERS
JP2000544822A JP4511033B2 (en) 1998-04-17 1999-04-16 Method for screening for bioactive agents capable of altering cell phenotype using FACS
EP99917563A EP1071809B1 (en) 1998-04-17 1999-04-16 Multiparameter facs assays to detect alterations in cellular parameters

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US09/062,330 1998-04-17
US09/062,330 US6897031B1 (en) 1998-04-17 1998-04-17 Multiparameter FACS assays to detect alterations in exocytosis
US09/157,748 US6461813B2 (en) 1998-09-21 1998-09-21 Multiparameter FACS assays to detect alterations in cell cycle regulation
US09/157,748 1998-09-21

Publications (2)

Publication Number Publication Date
WO1999054494A2 WO1999054494A2 (en) 1999-10-28
WO1999054494A3 true WO1999054494A3 (en) 2000-02-10

Family

ID=26742137

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/008345 WO1999054494A2 (en) 1998-04-17 1999-04-16 Multiparameter facs assays to detect alterations in cellular parameters

Country Status (12)

Country Link
EP (2) EP1071809B1 (en)
JP (1) JP4511033B2 (en)
CN (1) CN100334227C (en)
AT (1) ATE248226T1 (en)
AU (1) AU3565499A (en)
CA (1) CA2325597C (en)
DE (1) DE69910756T2 (en)
DK (1) DK1071809T3 (en)
ES (1) ES2207203T3 (en)
NZ (1) NZ508120A (en)
PT (1) PT1071809E (en)
WO (1) WO1999054494A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8309306B2 (en) 2008-11-12 2012-11-13 Nodality, Inc. Detection composition
US8962263B2 (en) 2001-07-10 2015-02-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for detecting the activation state of multiple proteins in single cells
US9115384B2 (en) 2001-07-10 2015-08-25 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for detecting receptor-ligand interactions in single cells

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US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US7090976B2 (en) 1999-11-10 2006-08-15 Rigel Pharmaceuticals, Inc. Methods and compositions comprising Renilla GFP
US6651008B1 (en) 1999-05-14 2003-11-18 Cytokinetics, Inc. Database system including computer code for predictive cellular bioinformatics
US6743576B1 (en) 1999-05-14 2004-06-01 Cytokinetics, Inc. Database system for predictive cellular bioinformatics
US7151847B2 (en) 2001-02-20 2006-12-19 Cytokinetics, Inc. Image analysis of the golgi complex
DE60135652D1 (en) * 2000-06-01 2008-10-16 Pasteur Institut Chimeric GPF aequorin as a bioluminescent Ca ++ marker at single cell level
US7218764B2 (en) 2000-12-04 2007-05-15 Cytokinetics, Inc. Ploidy classification method
US7016787B2 (en) 2001-02-20 2006-03-21 Cytokinetics, Inc. Characterizing biological stimuli by response curves
US6956961B2 (en) 2001-02-20 2005-10-18 Cytokinetics, Inc. Extracting shape information contained in cell images
US7393656B2 (en) 2001-07-10 2008-07-01 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for risk stratification
WO2005010677A2 (en) 2003-07-18 2005-02-03 Cytokinetics, Inc. Characterizing biological stimuli by response curves
US7235353B2 (en) 2003-07-18 2007-06-26 Cytokinetics, Inc. Predicting hepatotoxicity using cell based assays
US20050014217A1 (en) 2003-07-18 2005-01-20 Cytokinetics, Inc. Predicting hepatotoxicity using cell based assays
CN1860363B (en) * 2003-08-28 2011-12-28 赛路拉公司 Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network
JP4454998B2 (en) * 2003-09-29 2010-04-21 株式会社ニコン Cell observation apparatus and cell observation method
US7323318B2 (en) 2004-07-15 2008-01-29 Cytokinetics, Inc. Assay for distinguishing live and dead cells
JP4557685B2 (en) * 2004-11-15 2010-10-06 独立行政法人理化学研究所 Fluorescent protein
EP1943332A4 (en) 2005-10-14 2011-04-13 Medimmune Inc Cell display of antibody libraries
FR2902440B1 (en) * 2006-06-19 2008-08-29 Univ Paris Curie METHOD OF DETECTING AND DETECTING CELLS IN CYTODIERESE
US20090098594A1 (en) 2007-08-21 2009-04-16 Nodality, Inc. Methods for diagnosis prognosis and methods of treatment
EP2304436A1 (en) 2008-07-10 2011-04-06 Nodality, Inc. Methods for diagnosis, prognosis and treatment
US9459246B2 (en) 2009-09-08 2016-10-04 Nodality, Inc. Induced intercellular communication
US20110193950A1 (en) * 2010-02-09 2011-08-11 Stephen Liye Chen Application of microscopic linear array scanner for measuring rate of change of live organisms
CA2806294C (en) * 2010-07-28 2018-06-12 Canvax Biotech Sl Novel ultrasensitive cell based sensors and uses thereof
US9005909B2 (en) 2011-01-06 2015-04-14 Rigel Pharmaceuticals, Inc. Whole blood assay for measuring AMPK activation
US9766224B2 (en) 2015-03-25 2017-09-19 The Board Of Trustees Of The Leland Stanford Junior University Single cell analysis using secondary ion mass spectrometry
CA3020542A1 (en) 2016-04-11 2017-10-19 Board Of Regents, The University Of Texas System Methods and compositions for detecting single t cell receptor affinity and sequence
CN110412179A (en) * 2018-04-26 2019-11-05 缪荣明 A kind of method of liquid chromatographic detection granzyme A
EP3880838A4 (en) 2018-11-15 2022-11-02 The Board of Trustees of the Leland Stanford Junior University Compositions and methods of prognosis and classification for preeclampsia
WO2021079656A1 (en) * 2019-10-24 2021-04-29 国立大学法人千葉大学 Method for measuring activity of lipid-metabolizing enzyme in cell, and substance for use in measurement of activity of lipid-metabolizing enzyme in cell
EP4343775A1 (en) 2022-09-22 2024-03-27 SurgeCare A method for determining a medical outcome for an individual, related electronic system and computer program

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US4304720A (en) * 1980-04-30 1981-12-08 Merck & Co., Inc. Fluorescein esters and ethers and the preparation thereof
WO1991002085A1 (en) * 1989-08-10 1991-02-21 Housey Gerard M Method of screening for protein inhibitors and activators
EP0415695A1 (en) * 1989-08-30 1991-03-06 Eli Lilly And Company Selective cloning for high monoclonal antibody secreting hybridoma
EP0469632A1 (en) * 1990-08-03 1992-02-05 Systemix, Inc. Modulated xenogeneic organ systems in a immunocompromised host
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8962263B2 (en) 2001-07-10 2015-02-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for detecting the activation state of multiple proteins in single cells
US9115384B2 (en) 2001-07-10 2015-08-25 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for detecting receptor-ligand interactions in single cells
US8309306B2 (en) 2008-11-12 2012-11-13 Nodality, Inc. Detection composition

Also Published As

Publication number Publication date
DE69910756D1 (en) 2003-10-02
EP1071809B1 (en) 2003-08-27
PT1071809E (en) 2004-01-30
ATE248226T1 (en) 2003-09-15
WO1999054494A2 (en) 1999-10-28
DK1071809T3 (en) 2003-12-01
EP1363127A3 (en) 2004-03-24
JP2002512362A (en) 2002-04-23
EP1071809A2 (en) 2001-01-31
ES2207203T3 (en) 2004-05-16
CN100334227C (en) 2007-08-29
CA2325597A1 (en) 1999-10-28
CN1304489A (en) 2001-07-18
JP4511033B2 (en) 2010-07-28
DE69910756T2 (en) 2004-07-08
EP1363127A2 (en) 2003-11-19
NZ508120A (en) 2003-10-31
CA2325597C (en) 2010-09-21
AU3565499A (en) 1999-11-08

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