WO1999028463A3 - Methods for the degradation and detoxification of organic material using urine produced by transgenic animals - Google Patents

Methods for the degradation and detoxification of organic material using urine produced by transgenic animals Download PDF

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Publication number
WO1999028463A3
WO1999028463A3 PCT/US1998/025193 US9825193W WO9928463A3 WO 1999028463 A3 WO1999028463 A3 WO 1999028463A3 US 9825193 W US9825193 W US 9825193W WO 9928463 A3 WO9928463 A3 WO 9928463A3
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WO
WIPO (PCT)
Prior art keywords
urine
protein
organic material
human transgenic
transgenic animal
Prior art date
Application number
PCT/US1998/025193
Other languages
French (fr)
Other versions
WO1999028463A2 (en
Inventor
Henryk Lubon
Rekha Paleyanda
William Drohan
William Velander
Original Assignee
American Nat Red Cross
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Nat Red Cross filed Critical American Nat Red Cross
Priority to EP98960480A priority Critical patent/EP1036176A2/en
Priority to JP2000523339A priority patent/JP2001525171A/en
Priority to AU16064/99A priority patent/AU1606499A/en
Publication of WO1999028463A2 publication Critical patent/WO1999028463A2/en
Publication of WO1999028463A3 publication Critical patent/WO1999028463A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Environmental Sciences (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Medicinal Chemistry (AREA)
  • Processing Of Solid Wastes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method of producing a protein that degrades or detoxifies organic material is described. This method involves producing a non-human transgenic animal that produces such protein in its urine, and has stably integrated into its genome an exogenous gene encoding a protein that is detectable in the urine. Thus, a non-human transgenic animal that produces such protein in its urine, and a method of degrading or detoxifying organic materials also is described. Also a facility comprising a non-human transgenic animal that produces in its urine a protein that degrades or detoxifies organic material and a structure containing such animal is described. A method of altering a substance naturally found in urine is described. A DNA construct used in producing the non-human transgenic animal also is described.
PCT/US1998/025193 1997-12-01 1998-12-01 Methods for the degradation and detoxification of organic material using urine produced by transgenic animals WO1999028463A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP98960480A EP1036176A2 (en) 1997-12-01 1998-12-01 Methods for the degradation and detoxification of organic material using urine produced by transgenic animals
JP2000523339A JP2001525171A (en) 1997-12-01 1998-12-01 Method for decomposing and detoxifying organic materials using urine produced by transgenic animals
AU16064/99A AU1606499A (en) 1997-12-01 1998-12-01 Methods for the degradation and detoxification of organic material using urine produced by transgenic animals

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/982,284 US20020184655A1 (en) 1997-12-01 1997-12-01 Methods for the degradation and detoxification of organic material using urine produced by transgenic animals and related transgenic animals and proteins
US08/982,284 1997-12-01

Publications (2)

Publication Number Publication Date
WO1999028463A2 WO1999028463A2 (en) 1999-06-10
WO1999028463A3 true WO1999028463A3 (en) 1999-09-10

Family

ID=25529003

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/025193 WO1999028463A2 (en) 1997-12-01 1998-12-01 Methods for the degradation and detoxification of organic material using urine produced by transgenic animals

Country Status (5)

Country Link
US (1) US20020184655A1 (en)
EP (1) EP1036176A2 (en)
JP (1) JP2001525171A (en)
AU (1) AU1606499A (en)
WO (1) WO1999028463A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6888047B1 (en) 1998-11-13 2005-05-03 New York University Transgenic animals as urinary bioreactors for the production of polypeptide in the urine, recombinant DNA construct for kidney-specific expression, and method of using same
KR100453571B1 (en) * 2001-07-24 2004-10-20 대한민국 Transgenic porcine capable of producing human erythropoietin and preparation method thereof
US20030198959A1 (en) * 2002-03-28 2003-10-23 Kurnit David M. Methods and compositions for analysis of urine samples in the diagnosis and treatment of kidney diseases
CA2521064A1 (en) * 2003-03-31 2004-10-14 Council Of Scientific And Industrial Research Composting composition comprising animal urine and agricultural waste and method for vermicomposting the same
US20060194299A1 (en) * 2005-02-04 2006-08-31 Henrik Brinch-Pedersen Method for recycling important nutritional elements from waste
US7384556B2 (en) * 2005-07-19 2008-06-10 The University Of Wyoming Research Corporation Methods of enhancing biodegradation of groundwater contaminants
JP2013526840A (en) 2009-12-21 2013-06-27 ファーマシーネ，インコーポレイテッド Recombinant butyrylcholinesterase and its truncated form
WO2019045049A1 (en) * 2017-09-01 2019-03-07 学校法人帝京大学 Renal tubule cell-specific expression vector

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0051876A2 (en) * 1980-11-12 1982-05-19 Thoren Caging Systems, Inc. Animal caging system
WO1992006590A1 (en) * 1990-10-10 1992-04-30 The United States Of America, Represented By The Secretary, United States Department Of Commerce Cage configuration for arboreal reptiles
WO1995022249A1 (en) * 1994-02-18 1995-08-24 American National Red Cross Transgenic fibrinogen
FR2717500A1 (en) * 1994-03-17 1995-09-22 Pasteur Institut Use of recombinant vector encoding specific protein to transform kidney cells
WO1996039494A1 (en) * 1995-06-05 1996-12-12 New York University Method for expression and isolation of biologically active molecules in urine
EP0749876A1 (en) * 1995-06-20 1996-12-27 Automobiles Peugeot Motor vehicle brake system

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5057420A (en) * 1987-06-05 1991-10-15 Granada Biosciences, Inc. Bovine nuclear transplantation
US5453366A (en) * 1990-07-26 1995-09-26 Sims; Michele M. Method of cloning bovine embryos
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
US6613544B1 (en) * 1995-12-22 2003-09-02 Amgen Inc. Osteoprotegerin

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0051876A2 (en) * 1980-11-12 1982-05-19 Thoren Caging Systems, Inc. Animal caging system
WO1992006590A1 (en) * 1990-10-10 1992-04-30 The United States Of America, Represented By The Secretary, United States Department Of Commerce Cage configuration for arboreal reptiles
WO1995022249A1 (en) * 1994-02-18 1995-08-24 American National Red Cross Transgenic fibrinogen
FR2717500A1 (en) * 1994-03-17 1995-09-22 Pasteur Institut Use of recombinant vector encoding specific protein to transform kidney cells
WO1996039494A1 (en) * 1995-06-05 1996-12-12 New York University Method for expression and isolation of biologically active molecules in urine
EP0749876A1 (en) * 1995-06-20 1996-12-27 Automobiles Peugeot Motor vehicle brake system

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
"METHODS FOR THE DEGRADATION AND DETOXIFICATION OF ORGANIC MATERIAL USING URINE PRODUCED BY TRANSGENIC ANIMALS AND RELATED TRANSGENIC ANIMALS AND PROTEINS", XP002099574, Retrieved from the Internet <URL:http://www.vtip.org/Licensing%20O...tes/vtip%20disclosures/98-011.htm> [retrieved on 19990904] *

Also Published As

Publication number Publication date
JP2001525171A (en) 2001-12-11
EP1036176A2 (en) 2000-09-20
AU1606499A (en) 1999-06-16
US20020184655A1 (en) 2002-12-05
WO1999028463A2 (en) 1999-06-10

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