WO1999019467A1 - α-AMYLASE MUTANTS - Google Patents
α-AMYLASE MUTANTS Download PDFInfo
- Publication number
- WO1999019467A1 WO1999019467A1 PCT/DK1998/000444 DK9800444W WO9919467A1 WO 1999019467 A1 WO1999019467 A1 WO 1999019467A1 DK 9800444 W DK9800444 W DK 9800444W WO 9919467 A1 WO9919467 A1 WO 9919467A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- amylase
- seq
- variant
- termamyl
- parent
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
Definitions
- a variant of a parent Termamyl-like ⁇ -amylase with ⁇ -amylase activity comprising mutations in two, three, four, five or six of the following regions/positions or in corresponding positions in other parent Termamyl-like ⁇ -amylases: (relative to SEQ ID NO: 1) : 1: R181*, G182*, T183*, G184*
- N190A R, D,C,E,Q,G,H,I,L,K,M,F,P,S,T, ,Y,V I201A R,D,N,C,E,Q,G,H,L,K,M,F,P,S,T,W,Y,V D207A R,N,C,E,Q,G,H,I,L,K,M,F,P,S,T, ,Y,V E211A R,D,N,C,Q,G,H,I,L,K,M,F, P,S,T, ,Y,V Q264A R,D,N,C,E,G,H,I,L,K,M,F,P,S,T,W,Y,V
- R176* G177*,E178,G179* 2 N190A R,D,C,E,Q,G,H,I,L,K,M,F,P,S,T,W,Y,V; 3: V201A R,D,N,C,E,Q,G,H,I,L,K,M,F,P,S,T, ,Y; 53
- variant according to claim 1 comprising the following mutations: N190F/Q264S in SEQ ID NO: 4 or in corresponding positions in another parent ⁇ -amylase.
- the parent hybrid ⁇ -amylase is a hybrid alpha-amylase comprising the 445 C- terminal amino acid residues of the B . licheniformis ⁇ -amylase shown in SEQ ID NO: 4 and the 37 N-terminal amino acid residues of the ⁇ -amylase derived from B . amyloliquefaciens shown in SEQ ID NO: 5.
- a DNA construct comprising a DNA sequence encoding an ⁇ - amylase variant according to any one of claims 1 to 8.
- a recombinant expression vector which carries a DNA construct according to claim 9.
- the cell according to claim 13 which is a grampositive bacterium such as Bacillus subtilis, Bacillus licheniformis, Bacillus lentus, Bacillus brevis, Bacillus stearothermophilus, Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus coagulans, Bacillus circulans, Bacillus lautus or Bacillus thu- ringiensis.
- Bacillus subtilis Bacillus subtilis, Bacillus licheniformis, Bacillus lentus, Bacillus brevis, Bacillus stearothermophilus, Bacillus alkalophilus, Bacillus amyloliquefaciens, Bacillus coagulans, Bacillus circulans, Bacillus lautus or Bacillus thu- ringiensis.
- a detergent additive comprising an ⁇ -amylase variant accor- ding to any one of claims 1 to 8, optionally in the form of a non-dusting granulate, stabilised liquid or protected enzyme.
- a detergent additive according to claim 15 which contains 0.02-200 mg of enzyme protein/g of the additive.
- lipase a peroxidase, another amylolytic enzyme and/or a cellulase.
- a detergent composition comprising an ⁇ -amylase variant ac- cording to any of claims 1 to 8.
- the detergent composition according to claim 18 which additionally comprises another enzyme such as a protease, a lipase, a peroxidase, another amylolytic enzyme and/or a cellulase.
- another enzyme such as a protease, a lipase, a peroxidase, another amylolytic enzyme and/or a cellulase.
- a manual or automatic dishwashing detergent composition comprising an ⁇ -amylase variant according to any one of claims 1 to 8.
- a dishwashing detergent composition according to claim 20 which additionally comprises another enzyme such as a protease, a lipase, a peroxidase, another amylolytic enzyme and/or a cellulase.
- another enzyme such as a protease, a lipase, a peroxidase, another amylolytic enzyme and/or a cellulase.
- a manual or automatic laundry washing composition comprising an ⁇ -amylase variant according to any one of claims 1 to 8.
- another enzyme such as a protease, a lipase, a peroxidase, an amylolytic enzyme and/or a cellulase.
- composition comprising:
- Termamyl-like ⁇ -amylase the B. stearothermophilus ⁇ -amylase having the sequence shown in SEQ ID NO: 3; or
- a composition comprising: a mixture of one or more variants according any of claims 1 to 8 derived from (as the parent Termamyl-like ⁇ -amylase) the B. stearothermophilus ⁇ -amylase having the sequence shown in SEQ ID NO: 3 and a Termamyl-like alpha-amylase derived from the B. licheniformis ⁇ -amylase having the sequence shown in SEQ ID NO: 4.
- composition comprising: a mixture of one or more variants according any of claims 1 to 8 derived from (as the parent Termamyl-like ⁇ -amylase) the B. stearothermophilus ⁇ -amylase having the sequence shown in SEQ ID NO: 3 and a hybrid alpha-amylase comprising a part of the B . amyloliquefaciens ⁇ -amylase shown in SEQ ID NO: 5 and a part of the B. licheniformis ⁇ -amylase shown in SEQ ID NO: 4.
- composition according to claim 26, wherein the hybrid ⁇ - amylase is a hybrid alpha-amylase comprising the 445 C-terminal amino acid residues of the B . licheniformis ⁇ -amylase shown in
- SEQ ID NO: 4 and the 37 N-terminal amino acid residues of the ⁇ - amylase derived from B . amyloliquefaciens shown in SEQ ID NO: 5.
- a method for generating a variant of a parent Termamyl-like ⁇ -amylase, which variant exhibits increased stability at low pH and at low calcium concentration relative to the parent comprising:
- step (b) expressing the mutated DNA sequence obtained in step (a) in a host cell
- the present invention relates, inter alia , to novel variants
- mutants of parent Termamyl-like ⁇ -amylases notably variants exhibiting increased thermostability at acidic pH and/or at low Ca 2+ concentrations (relative to the parent) which are advantageous with respect to applications of the variants in, industrial starch processing particularly (e.g. starch liquefaction or saccharification) .
- ⁇ -Amylases ( ⁇ -1, 4-glucan-4-glucanohydrolases, EC 3.2.1.1) constitute a group of enzymes which catalyze hydrolysis of starch and other linear and branched 1, -glucosidic oligo- and polysaccharides .
- ⁇ -amylase such as Termamyl-like ⁇ -amylases variants are known from e.g. WO 90/11352, WO 95/10603, WO 95/26397, WO 96/23873 and WO 96/23874.
- 96/23874 provides three-dimensional, X-ray crystal structural data for a Termamyl-like ⁇ -amylase which consists of the 300 N- terminal amino acid residues of the B. amyloliquefaciens ⁇ -amylase and amino acids 301-483 of the C-terminal end of the
- B. licheniformis ⁇ -amylase comprising the amino acid sequence
- TermamylTM Bacillus ⁇ -amylases
- Bacillus ⁇ -amylases Bacillus ⁇ -amylases
- Termamyl- like ⁇ -amylases Bacillus ⁇ -amylases
- WO 96/23874 further describes methodology for designing, on the basis of an analysis of the structure of a parent Termamyl-like ⁇ -amylase, variants of the parent Termamyl- like ⁇ -amylase which exhibit altered properties relative to the parent .
- WO 95/35382 (Gist Brocades B.V.) concerns amylolytic enzymes derived from B. licheniformis with improved properties allowing reduction of the Ca 2+ concentration under application without a loss of performance of the enzyme.
- the amylolytic enzyme comprises one or more amino acid changes at positions selected from the group of 104, 128, 187, 188 of the B. licheniformis ⁇ - amylase sequence.
- WO 96/23873 discloses Termamyl-like ⁇ -amylase variants which have increased thermostability obtained by pairwise deletion in the region R181*, G182*, T183* and G184* of the sequence shown in SEQ ID NO: 1 herein.
- the present invention relates to novel ⁇ -amylolytic variants
- mutants of a Termamyl-like ⁇ -amylase in particular variants exhibiting increased thermostability (relative to the parent) which are advantageous in connection with the industrial processing of starch (starch liquefaction, saccharification and the like) .
- thermostability of Termamyl-like ⁇ - amylases is increased at acidic pH and/or at low Ca 2+ concentration in comparison to single mutations, such as the mutation dislcosed in WO 96/23873 (Novo Nordisk), i.e. pairwise deletion in the region R181*, G182*, T183* and G184* of the sequence shown in SEQ ID NO: 1 herein.
- the invention further relates to DNA constructs encoding variants of the invention, to composition comprising variants of the invention, to methods for preparing variants of the invention, and to the use of variants and compositions of the invention, alone or in combination with other ⁇ -amylolytic enzymes, m various industrial processes, e . g. starch liquefaction.
- Figure 1 is an alignment of the amino acid sequences of six parent Termamyl-like ⁇ -amylases in the context of the invention.
- the numbers on the Extreme left designate the respective amino acid sequences as follows:
- TermamylTM has been found to be about 89% homologous with the B. amyloliquefaciens ⁇ -amylase comprising the amino acid sequence shown in SEQ ID NO: 5 and about 79% homologous with the B . stearothermophilus ⁇ -amylase comprising the amino acid sequence shown in SEQ ID NO: 3. Further homologous ⁇ -amylases include an ⁇ -amylase derived from a strain of the Bacillus sp.
- NCIB 12289, NCIB 12512, NCIB 12513 or DSM 9375 all of which are described in detail in WO 95/26397, and the ⁇ -amylase described by Tsukamoto et al., Biochemical and Biophysical Research Communications, 151 (1988), pp. 25-31.
- Still further homologous ⁇ -amylases include the ⁇ -amylase produced by the B . licheniformis strain described in EP 0252666 (ATCC 27811), and the ⁇ -amylases identified in WO 91/00353 and WO 94/18314.
- Other commercial Termamyl-like B . licheniformis ⁇ -amylases are OptithermTM and TakathermTM (available from Solvay) , MaxamylTM (available from Gist-brocades/Genencor) , Spezym AATM and Spezyme Delta AATM (available from Genencor) , and KeistaseTM (available from Daiwa) .
- ⁇ - amylases Because of the substantial homology found between these ⁇ - amylases, they are considered to belong to the same class of ⁇ - amylases, namely the class of "Termamyl-like ⁇ -amylases".
- Termamyl-like ⁇ -amylase is intended to indicate an ⁇ -amylase which, at the amino acid level, exhibits a substantial homology to TermamylTM, i.e. the B. licheniformis ⁇ -amylase having the amino acid sequence shown in SEQ ID NO: 4 herein.
- a Termamyl-like ⁇ -amylase is an ⁇ -amylase which has the amino acid sequence shown in SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7 or 8 herein, and the amino acid sequence shown in SEQ ID NO: 1 of WO 95/26397 (the same as the amino acid sequence shown as SEQ ID NO: 7 herein) or in SEQ ID NO: 2 of WO 95/26397 (the same as the amino acid sequence shown as SEQ ID NO: 8 herein) or in Tsukamoto et al., 1988, (which amino acid sequence is shown in SEQ ID NO: 6 herein) or i) which displays at least 60%, preferred at least 70%, more preferred at least 75%, even more preferred at least 80%, especially at least 85%, especially preferred at least 90%, even especially more preferred at least 95% homology with at least one of said amino acid sequences shown in SEQ ID NOS 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 and/or ii) displays immunological cross-re
- the "homology" may be determined by use of any conventional algorithm, preferably by use of the GAP progamme from the GCG package version 7.3 (June
- GAP penalties which is a GAP creation penalty of 3.0 and GAP extension penalty of 0.1
- a structural alignment between Termamyl and a Termamyl-like ⁇ -amylase may be used to identify equivalent/corresponding positions in other Termamyl-like ⁇ -amylases.
- One method of obtaining said structural alignment is to use the Pile Up programme from the GCG package using default values of gap penalties, i.e., a gap creation penalty of 3.0 and gap extension penalty of 0.1.
- Other structural alignment methods include the hydrophobic cluster analysis (Gaboriaud et al . , (1987), FEBS LETTERS 224, pp. 149-155) and reverse threading (Huber, T ; Torda, AE, PROTEIN SCIENCE Vol. 7, No. 1 pp. 142-149 (1998).
- Property ii) of the ⁇ -amylase, i . e . the immunological cross reactivity may be assayed using an antibody raised against, or reactive with, at least one epitope of the relevant Termamyl- like ⁇ -amylase.
- the antibody which may either be monoclonal or polyclonal, may be produced by methods known in the art, e.g. as described by Hudson et al., Practical Immunology, Third edition
- the immunological cross-reactivity may be determined using assays known in the art, examples of which are Western Blotting or radial immunodif- fusion assay, e.g. as described by Hudson et al., 1989. In this respect, immunological cross-reactivity between the ⁇ -amylases having the amino acid sequences SEQ ID NOS: 1, 2, 3, 4, 5, 6, 7, or 8 respectively, have been found.
- Termamyl-like ⁇ -amylase in accordance with property iii) above may suitably be prepared on the basis of the full or partial nucleotide or amino acid sequence of the ⁇ -amylase in question.
- Suitable conditions for testing hybridization involve presoaking in 5xSSC and prehybridizing for 1 hour at ⁇ 40°C in a solution of 20% formamide, 5xDenhardt ' s solution, 50mM sodium phosphate, pH 6.8, and 50mg of denatured sonicated calf thymus DNA, followed by hybridization in the same solution supplemented with lOOmM ATP for 18 hours at ⁇ 40°C, followed by three times washing of the filter in 2xSSC, 0.2% SDS at 40°C for 30 minutes (low stringency) , preferred at 50°C (medium stringency) , more preferably at 65°C (high stringency) , even more preferably at
- derived from is intended not only to indicate an ⁇ -amylase produced or producible by a strain of the organism in question, but also an ⁇ -amylase encoded by a DNA sequence isolated from such strain and produced in a host organism transformed with said DNA sequence.
- the term is intended to indicate an ⁇ -amylase which is encoded by a DNA sequence of synthetic and/or cDNA origin and which has the identifying characteristics of the ⁇ -amylase in question.
- the parent ⁇ -amylase may be a variant of a naturally occurring ⁇ -amylase, i.e. a variant which is the result of a modification (insertion, substitution, deletion) of one or more amino acid residues of the naturally occurring ⁇ -amylase.
- the parent ⁇ -amylase may be a hybrid ⁇ -amylase, i.e. an ⁇ - amylase which comprises a combination of partial amino acid sequences derived from at least two ⁇ -amylases.
- the parent hybrid ⁇ -amylase may be one which on the basis of amino acid homology and/or immunological cross-reactivity and/or DNA hybridization (as defined above) can be determined to belong to the Termamyl-like ⁇ -amylase family.
- the hybrid ⁇ -amylase is typically composed of at least one part of a
- Termamyl-like ⁇ -amylase and part(s) of one or more other ⁇ - amylases selected from Termamyl-like ⁇ -amylases or non-Termamyl- like ⁇ -amylases of microbial (bacterial or fungal) and/or mammalian origin.
- the parent hybrid ⁇ -amylase may comprise a combination of partial amino acid sequences deriving from at least two Termamyl-like ⁇ -amylases, or from at least one Termamyl-like and at least one non-Termamyl-like bacterial ⁇ -amylase, or from at least one Termamyl-like and at least one fungal ⁇ -amylase.
- Termamyl-like ⁇ -amylase from which a partial amino acid sequence derives may, e.g., be any of those specific Termamyl-like ⁇ - amylases referred to herein.
- the parent ⁇ -amylase may comprise a C-terminal part of an ⁇ -amylase derived from a strain of B . licheniformis , and a N-terminal part of an ⁇ -amylase derived from a strain of
- the parent ⁇ -amylase may comprise at least 430 amino acid residues of the C-terminal part of the B . licheniformis ⁇ -amylase, and may, e.g. comprise a) an amino acid segment corresponding to the 37 N-terminal amino acid residues of the B . amyloliquefaciens ⁇ -amylase having the amino acid sequence shown in SEQ ID NO: 5 and an amino acid segment corresponding to the 445 C-terminal amino acid residues of the
- B . licheniformis ⁇ -amylase having the amino acid sequence shown in SEQ ID No. 4, or b) an amino acid segment corresponding to the 68 N-terminal amino acid residues of the B . stearother- mophilus ⁇ -amylase having the amino acid sequence shown in SEQ ID NO: 3 and an amino acid segment corresponding to the 415 C- terminal amino acid residues of the B . licheniformis ⁇ -amylase having the amino acid sequence shown in SEQ ID NO: 4. 8
- the non-Termamyl-like ⁇ -amylase may, e.g., be a fungal ⁇ - amylase, a mammalian or a plant ⁇ -amylase or a bacterial ⁇ - amylase (different from a Termamyl-like ⁇ -amylase) .
- Specific examples of such ⁇ -amylases include the Aspergillus oryzae TAKA ⁇ -amylase, the A. niger acid ⁇ -amylase, the Bacillus subtilis ⁇ - amylase, the porcine pancreatic ⁇ -amylase and a barley ⁇ - amylase. All of these ⁇ -amylases have elucidated structures which are markedly different from the structure of a typical
- Termamyl-like ⁇ -amylase as referred to herein.
- the fungal ⁇ -amylases mentioned above, i.e. derived from A. niger and A. oryzae, are highly homologous on the amino acid level and generally considered to belong to the same family of ⁇ -amylases.
- the fungal ⁇ -amylase derived from Aspergillus oryzae is commercially available under the tradename FungamylTM.
- FungamylTM a particular variant of a Termamyl-like ⁇ - amylase (variant of the invention) is referred to - in a conventional manner - by reference to modification (e.g.
- a preferred embodiment of a variant of the invention is one derived from a B. licheniformis ⁇ -amylase (as parent Termamyl- like ⁇ -amylase), e.g. one of those referred to above, such as the B . licheniformis ⁇ -amylase having the amino acid sequence shown in SEQ ID NO: 4.
- the construction of the variant of interest may be accomplished by cultivating a microorganism comprising a DNA sequence encoding the variant under conditions which are conducive for producing the variant. The variant may then subsequently be recovered from the resulting culture broth. This is described in detail further below.
- Mutations of particular relevance in relation to obtaining variants according to the invention having increased thermostability at acidic pH and/or at low Ca 2+ concentration include mutations at the following positions (relative to B. licheniformis ⁇ -amylase, SEQ ID NO: 4):
- acidic pH means a pH below 7.0, especially below the pH range, in which industrial starch liquefaction processes are normally performed, which is between pH 5.5 and 6.2.
- low Calcium concentration means concentrations below the normal level used in industrial starch liquefaction. Normal concentrations vary depending of the concentration of free Ca 2+ in the corn. Normally a dosage corresponding to ImM (40ppm) is added which together with the level in corn gives between 40 and 60ppm free Ca 2+
- high tempertatures means temperatures between 95°C and 160°C, especially the temperature range in which industrial starch liquefaction processes are normally performed, which is between 95°C and
- thermostability at acidic pH and/or at low Ca 2+ concentration may be increased even more by combining certain mutations including the above 10
- Said "certain" mutations are the following (relative to B . licheniformis ⁇ -amylase, SEQ ID NO: 4): N190, D207, E211, Q264 and 1201. Said mutation may further be combined with deletions in one, preferably two or even three positions as described in WO 96/23873 (i.e. in positions R181, G182, T183, G184 in SEQ ID NO: 1 herein) . According to the invention variants of a parent
- Termamyl-like ⁇ -amylase with ⁇ -amylase activity comprising mutations in two, three, four, five or six of the above positions are contemplated.
- Termamyl-like ⁇ - amylases mentioned specifically below are contemplated. Also other commercial Termamyl-like ⁇ -amylases are contemplated. An unexhaustive list of such ⁇ -amylases is the following: ⁇ -amylases produced by the B. licheniformis strain described in
- EP 0252666 (ATCC 27811), and the ⁇ -amylases identified in WO 91/00353 and WO 94/18314.
- Other commercial Termamyl-like B . licheniformis ⁇ -amylases are OptithermTM and TakathermTM (available from Solvay) , MaxamylTM (available from Gist- brocades/Genencor) , Spezym AATM Spezyme Delta AATM (available from
- KeistaseTM available from Daiwa
- amino acid residues respectively, at positions corresponding to N190, 1201, D207 and E211, respectively, in SEQ ID NO: 4 constitute amino acid residues which are conserved in numerous Termamyl-like ⁇ - amylases.
- amino acid residues respectively, at positions corresponding to N190, 1201, D207 and E211, respectively, in SEQ ID NO: 4 constitute amino acid residues which are conserved in numerous Termamyl-like ⁇ - amylases.
- Termamyl-like ⁇ -amylases which have already been mentioned ( vide supra) are as follows:
- amyloliquefaciens (SEQ ID No.5) R176, G177, E178, G179
- Bacillus WO 95/26397 (SEQ ID No.l) R181, G182, D183, G184 "Bacillus sp. #707" (SEQ ID No.6) R181, G182, H183, G184
- SEQ ID NO: 1 to SEQ ID NO: 6 as the backbone (i.e. as the parent Termamyl-like ⁇ -amylase) two, three, four, five or six mutations may according to the invention be made in the following regions/positions to increase the thermostability at acidic pH and/or at low Ca 2+ concentrations (relative to the parent) : (relative to Seq ID NO: 1 herein) : 12
- Comtemplated according to the present invention is combining three, four, five or six mutation.
- Termamyl-like ⁇ -amylase defined above may suitably be used as backbone for preparing variants of the invention.
- the variant comprises the following mutations: N190F/Q264S in SEQ ID NO: 4 or in corresponding positiones in another parent Termamyl-like ⁇ - amylases .
- the variant of the invention comprises 19
- Termamyl-like ⁇ -amylases Said variant may further comprise a substitution in position E214Q.
- the parent in a preferred embodiment of the invention the parent
- Termamyl-like ⁇ -amylase is a hybrid ⁇ -amylase of SEQ ID NO: 4 and SEQ ID NO: 5.
- the parent hybrid Termamyl-like ⁇ -amylase may be a hybrid alpha-amylase comprising the 445 C- terminal amino acid residues of the B . licheniformis ⁇ -amylase shown in SEQ ID NO: 4 and the 37 N-terminal amino acid residues of the ⁇ -amylase derived from B . amyloliquefaciens shown in SEQ ID NO: 5, which may suitably further have the following mutations: H156Y+A181T+N190F+A209V+Q264S (using the numbering in SEQ ID NO: 4) .
- the latter mentioned hybrid is used in the examples below and is referred to as LE174.
- a variant of the invention comprises one or more modifications in addition to those outlined above.
- one or more proline residues present in the part of the ⁇ -amylase variant which is modified is/are replaced with a non-proline residue which may be any of the possible, naturally occurring non-proline residues, and which preferably is an alanine, glycine, serine, threonine, valine or leucine.
- cysteine residues present among the amino acid residues with which the parent ⁇ -amylase is modified is/are replaced with a non-cysteine residue such as serine, alanine, threonine, glycine, valine or leucine.
- a variant of the invention may - either as the only modification or in combination with any of the above outlined modifications - be modified so that one or more Asp and/or Glu present in an amino acid fragment corresponding to the amino acid fragment 185-209 of SEQ ID NO: 4 is replaced by an Asn and/or Gin, respectively. Also of interest is the 20
- the DNA sequence encoding a parent ⁇ -amylase may be isolated from any cell or microorganism producing the ⁇ -amylase in question, using various methods well known in the art.
- a genomic DNA and/or cDNA library should be constructed using chromosomal DNA or messenger RNA from the organism that produces the ⁇ -amylase to be studied.
- homologous, labelled oligonucleotide probes may be synthesized and used to identify ⁇ -amylase- encoding clones from a genomic library prepared from the organism in question.
- a labelled oligonucleotide probe containing sequences homologous to a known ⁇ -amylase gene could be used as a probe to identify ⁇ -amylase-encoding clones, using hybridization and washing conditions of lower stringency.
- Yet another method for identifying ⁇ -amylase-encoding clones would involve inserting fragments of genomic DNA into an expression vector, such as a plasmid, transforming ⁇ -amylase- negative bacteria with the resulting genomic DNA library, and 21
- DNA sequence encoding the enzyme may be prepared synthetically by established standard methods, e.g. the phosphoroamidite method described by S.L. Beaucage and M.H.
- oligonucleotides are synthesized, e.g. in an automatic DNA synthesizer, purified, annealed, ligated and cloned in appropriate vectors.
- the DNA sequence may be of mixed genomic and synthetic origin, mixed synthetic and cDNA origin or mixed genomic and cDNA origin, prepared by ligating fragments of synthetic, genomic or cDNA origin (as appropriate, the fragments corresponding to various parts of the entire DNA sequence) , in accordance with standard techniques.
- the DNA sequence may also be prepared by polymerase chain reaction (PCR) using specific primers, for instance as described in US 4,683,202 or R.K. Saiki et al. (1988) .
- mutations may be introduced using synthetic oligonucleotides. These oligonucleotides contain nucleotide sequences flanking the desired mutation sites; mutant nucleotides are inserted during oligonucleotide synthesis.
- a single-stranded gap of DNA, bridging the ⁇ -amylase-encoding sequence is created in a vector carrying the ⁇ -amylase gene.
- the synthetic nucleotide, bearing the desired mutation is annealed to a homologous portion of the single-stranded DNA.
- mutations by performing minor alterations of the cassette.
- an even greater variety of mutations can be introduced at any one time by the Morinaga method, because a multitude of oligonucleotides, of various lengths, can be introduced.
- Another method for introducing mutations into ⁇ -amylase- encoding DNA sequences is described in Nelson and Long (1989) . It involves the 3 -step generation of a PCR fragment containing the desired mutation introduced by using a chemically synthesized DNA strand as one of the primers in the PCR reactions. From the PCR-generated fragment, a DNA fragment carrying the mutation may be isolated by cleavage with restriction endonucleases and reinserted into an expression plasmid.
- Random mutagenesis is suitably performed either as localised or region-specific random mutagenesis in at least three parts of the gene translating to the amino acid sequence shown in question, or within the whole gene.
- the random mutagenesis of a DNA sequence encoding a parent ⁇ - amylase may be conveniently performed by use of any method known in the art.
- a further aspect of the present invention relates to a method for generating a variant of a parent ⁇ -amylase, e.g. wherein the variant exhibits altered or increased thermal stability relative to the parent, the method comprising:
- step (a) subjecting a DNA sequence encoding the parent ⁇ -amylase to random mutagenesis, (b) expressing the mutated DNA sequence obtained in step (a) in a host cell, and
- Step (a) of the above method of the invention is preferably performed using doped primers.
- the random mutagenesis may be performed by use of a suitable physical or chemical mutagenizing agent, by use of a suitable oligonucleotide, or by subjecting the DNA sequence to PCR generated mutagenesis.
- the random mutagenesis may be performed by use of any combination of these mutagenizing agents.
- the mutagenizing agent may, e . g. , be one which induces transitions, transversions, inversions, scrambling, deletions, and/or insertions.
- Examples of a physical or chemical mutagenizing agent suitable for the present purpose include ultraviolet (UV) ir-radiation, hydroxylamine, N-methyl-N ' -nitro-N-nitrosoguanidine (MNNG) , 0- methyl hydroxylamine, nitrous acid, ethyl methane sulphonate (EMS), sodium bisulphite, formic acid, and nucleotide analogues.
- UV ultraviolet
- MNNG N-methyl-N ' -nitro-N-nitrosoguanidine
- EMS ethyl methane sulphonate
- sodium bisulphite formic acid
- nucleotide analogues examples include ultraviolet (UV) ir-radiation, hydroxylamine, N-methyl-N ' -nitro-N-nitrosoguanidine (MNNG) , 0- methyl hydroxylamine, nitrous acid, ethyl methane sulphonate (EMS), sodium bisulphit
- the oligonucleotide may be doped or spiked with the three non-parent nucleotides during the synthesis of the oligonucleotide at the positions which are to be changed.
- the doping or spiking may be done so that codons for unwanted amino acids are avoided.
- the doped or spiked oligonucleotide can be incorporated into the DNA encoding the ⁇ -amylase enzyme by any published technique, using e.g. PCR, LCR or any DNA polymerase and ligase as deemed appropriate. 24
- the doping is carried out using "constant random doping", in which the percentage of wild-type and mutation in each position is predefined.
- the doping may be directed toward a preference for the introduction of certain nucleotides, and thereby a preference for the introduction of one or more specific amino acid residues.
- the doping may be made, e.g., so as to allow for the introduction of 90% wild type and 10% mutations in each position.
- An additional consideration in the choice of a doping scheme is based on genetic as well as protein-structural constraints.
- the doping scheme may be made by using the DOPE program which, inter alia , ensures that introduction of stop codons is avoided.
- PCR-generated mutagenesis When PCR-generated mutagenesis is used, either a chemically treated or non-treated gene encoding a parent ⁇ -amylase is subjected to PCR under conditions that increase the mis- incorporation of nucleotides (Deshler 1992; Leung et al., Technique, Vol.l, 1989, pp. 11-15).
- DNA encoding the ⁇ -amylase by, e.g., transforming a plasmid containing the parent glycosylase into the mutator strain, growing the mutator strain with the plasmid and isolating the mutated plasmid from the mutator strain.
- the mutated plasmid may be subsequently transformed into the expression organism.
- the DNA sequence to be mutagenized may be conveniently present in a genomic or cDNA library prepared from an organism expressing the parent ⁇ -amylase.
- the DNA sequence may be present on a suitable vector such as a plasmid or a bacteriophage, which as such may be incubated with or other-wise exposed to the mutagenising agent.
- the DNA to be mutagenized may also be present in a host cell either by being integrated in the genome of said cell or by being present on a vector harboured in the cell.
- the DNA to be mutagenized may be in isolated form. It will be understood that the DNA sequence to be subjected to random mutagenesis is preferably a cDNA or a genomic DNA sequence. 25
- telomere amplification may be performed in accordance with methods known in the art, the presently preferred method being PCR-generated amplification using oligonucleotide primers prepared on the basis of the DNA or amino acid sequence of the parent enzyme.
- the mutated DNA is expressed by culturing a suitable host cell carrying the DNA sequence under conditions allowing expression to take place.
- the host cell used for this purpose may be one which has been transformed with the mutated DNA sequence, optionally present on a vector, or one which was carried the DNA sequence encoding the parent enzyme during the mutagenesis treatment.
- suitable host cells are the following: gram positive bacteria such as Bacillus subtilis, Bacillus licheniformis , Bacillus lentus, Bacillus brevis, Bacillus stearothermophilus , Bacillus alkalophilus, Bacillus amyloliquefaciens , Bacillus coagulans, Bacillus circulans, Bacillus lautus, Bacillus megaterium, Bacillus thuringiensis, Streptomyces lividans or Streptomyces murinus; and gram-negative bacteria such as E. coli .
- gram positive bacteria such as Bacillus subtilis, Bacillus licheniformis , Bacillus lentus, Bacillus brevis, Bacillus stearothermophilus , Bacillus alkalophilus, Bacillus amyloliquefaciens , Bacillus coagulans, Bacillus circulans, Bacillus lautus, Bacillus megaterium, Bacillus thuringiensis, Str
- the mutated DNA sequence may further comprise a DNA sequence encoding functions permitting expression of the mutated DNA sequence.
- the random mutagenesis may be advantageously localized to a part of the parent ⁇ -amylase in question. This may, e.g., be advantageous when certain regions of the enzyme have been identified to be of particular importance for a given property of the enzyme, and when modified are expected to result in a variant having improved properties. Such regions may normally be identified when the tertiary structure of the parent enzyme has been elucidated and related to the function of the enzyme.
- the localized, or region-specific, random mutagenesis is conveniently performed by use of PCR generated mutagenesis techniques as described above or any other suitable technique known in the art.
- the DNA sequence encoding the part of the DNA sequence to be modified may be isolated, e . g. , by insertion into a suitable vector, and said part may be subsequently subjected to mutagenesis by use of any of the mutagenesis methods discussed above.
- a DNA sequence encoding the variant produced by methods described above, or by any alternative methods known in the art can be expressed, in enzyme form, using an expression vector which typically includes control sequences encoding a promoter, operator, ribosome binding site, translation initiation signal, and, optionally, a repressor gene or various activator genes.
- the recombinant expression vector carrying the DNA sequence encoding an ⁇ -amylase variant of the invention may be any vector which may conveniently be subjected to recombinant DNA procedures, and the choice of vector will often depend on the host cell into which it is to be introduced.
- the vector may be an autonomously replicating vector, i.e. a vector which exists as an extrachromosomal entity, the replication of which is independent of chromosomal replication, e.g. a plasmid, a bacteriophage or an extrachromosomal element, minichromosome or an artificial chromosome.
- the vector may be one which, when introduced into a host cell, is integrated into the host cell genome and replicated together with the chromosome (s) into which it has been integrated.
- the DNA sequence should be operably connected 27
- the promoter may be any DNA sequence which shows transcriptional activity in the host cell of choice and may be derived from genes encoding proteins either homologous or heterologous to the host cell.
- suitable promoters for directing the transcription of the DNA sequence encoding an ⁇ -amylase variant of the invention, especially in a bacterial host are the promoter of the lac operon of E.
- the Streptomyces coelicolor agarase gene dagk promoters the promoters of the Bacillus licheniformis ⁇ -amylase gene ( amyL) , the promoters of the Bacillus stearothermophilus maltogenic amylase gene ( amyM) , the promoters of the Bacillus amyloliquefaciens ⁇ -amylase ( amyQ) , the promoters of the Ba cillus subtilis xylA and xylB genes etc.
- useful promoters are those derived from the gene encoding A.
- the expression vector of the invention may also comprise a suitable transcription terminator and, in eukaryotes, poly- adenylation sequences operably connected to the DNA sequence encoding the ⁇ -amylase variant of the invention. Termination and polyadenylation sequences may suitably be derived from the same sources as the promoter.
- the vector may further comprise a DNA sequence enabling the vector to replicate in the host cell in question.
- sequences are the origins of replication of plasmids pUC19, pACYC177, pUBHO, pE194, pAMBl and pIJ702.
- the vector may also comprise a selectable marker, e.g. a gene the product of which complements a defect in the host cell, such as the dal genes from B. subtilis or B. licheniformis, or one which confers antibiotic resistance such as ampicillin, kanamycin, chloramphenicol or tetracyclin resistance.
- the vector may comprise Aspergillus selection markers such as a dS, argB, niaD and sC, a marker giving rise to 28
- hygromycin resistance or the selection may be accomplished by co-transformation, e.g. as described in WO 91/17243.
- Bacillus ⁇ -amylases mentioned herein comprise a preregion permitting secretion of the expressed protease into the culture medium. If desirable, this preregion may be replaced by a different preregion or signal sequence, conveniently accom- plished by substitution of the DNA sequences encoding the respective preregions.
- the cell of the invention is advantageously used as a host cell in the recombinant production of an ⁇ -amylase variant of the invention.
- the cell may be transformed with the DNA construct of the invention encoding the variant, conveniently by integrating the DNA construct (in one or more copies) in the host chromosome. This integration is generally considered to be an advantage as the DNA sequence is more likely to be stably maintained in the cell. Integration of the DNA constructs into the host chromosome may be performed according to conventional methods, e.g. by homologous or heterologous recombination. Alternatively, the cell may be transformed with an expression vector as described above in connection with the different types of host cells.
- the cell of the invention may be a cell of a higher organism such as a mammal or an insect, but is preferably a microbial cell, e.g. a bacterial or a fungal (including yeast) cell.
- a microbial cell e.g. a bacterial or a fungal (including yeast) cell.
- suitable bacteria are grampositive bacteria such as Bacillus subtilis, Bacillus licheniformis, Bacillus lentus, 29
- Bacillus brevis Bacillus stearothermophilus , Bacillus alkalo- philus, Bacillus amyloliquefaciens , Bacillus coagulans, Bacillus circulans, Bacillus lautus, Bacillus megaterium, Bacillus thuringiensis , or Streptomyces lividans or Streptomyces murinus, or gramnegative bacteria such as E. coli .
- the transformation of the bacteria may, for instance, be effected by protoplast transformation or by using competent cells in a manner known per se.
- the yeast organism may favourably be selected from a species of Saccharomyces or Schizosaccharomyces, e . g. Saccharomyces cerevisiae .
- the filamentous fungus may advantageously belong to a species of Aspergillus, e . g. Aspergillus oryzae or Aspergillus niger.
- Fungal cells may be transformed by a process involving protoplast formation and transformation of the protoplasts followed by regeneration of the cell wall in a manner known per se. A suitable procedure for transformation of Aspergillus host cells is described in EP 238 023.
- the present invention relates to a method of producing an ⁇ -amylase variant of the invention, which method comprises cultivating a host cell as described above under conditions conducive to the production of the variant and recovering the variant from the cells and/or culture medium.
- the medium used to cultivate the cells may be any conventional medium suitable for growing the host cell in question and obtaining expression of the ⁇ -amylase variant of the invention. Suitable media are available from commercial suppliers or may be prepared according to published recipes ( e . g. as described in catalogues of the American Type Culture Collection) .
- the ⁇ -amylase variant secreted from the host cells may conveniently be recovered from the culture medium by well-known procedures, including separating the cells from the medium by centrifugation or filtration, and precipitating proteinaceous components of the medium by means of a salt such as ammonium sulphate, followed by the use of chromatographic procedures such as ion exchange chromatography, affinity chromatography, or the like.
- the ⁇ -amylase variants of this invention possesses valuable properties allowing for a variety of industrial applications.
- enzyme variants of the invention are applicable as a component in washing, dishwashing and hard-surface cleaning detergent compositions.
- Numerous variants are particularly useful in the production of sweeteners and ethanol from starch, and/or for textile desizing. Conditions for conventional starch- conversion processes, including starch liquefaction and/or saccharification processes, are described in, e.g., US 3,912,590 and in EP patent publications Nos. 252 730 and 63 909.
- a "traditional" process for conversion of starch to fructose syrups normally consists of three consecutive enzymatic processes, viz. a liquefaction process followed by a sacchari- fication process and an isomerization process.
- starch is degraded to dextrins by an ⁇ - amylase (e.g. TermamylTM) at pH values between 5.5 and 6.2 and at temperatures of 95-160°C for a period of approx. 2 hours.
- ⁇ - amylase e.g. TermamylTM
- 1 mM of calcium is added (40 ppm free calcium ions) .
- the dextrins are converted into dextrose by addition of a glucoamylase (e.g. AMGTM) and a debranching enzyme, such as an isoamylase or a pullulanase ( e . g. PromozymeTM) .
- a glucoamylase e.g. AMGTM
- a debranching enzyme such as an isoamylase or a pullulanase (e . g. PromozymeTM)
- the pH is reduced to a value below 4.5, maintaining the high temperature (above 95°C) , and the liquefying ⁇ -amylase activity is denatured.
- the temperature is lowered to 60 °C, and glucoamylase and debranching enzyme are added.
- the saccharification process proceeds for 24-72 hours.
- the pH is increased to a value in the range of 6-8, preferably pH 7.5, and the calcium is removed by ion exchange.
- the dextrose syrup is then converted into high fructose syrup using, e.g., an immmobilized gluco- seisomerase (such as SweetzymeTM) .
- an immmobilized gluco- seisomerase such as SweetzymeTM
- At least 1 enzymatic improvements of this process could be envisaged.
- Reduction of the calcium dependency of the liquefying ⁇ -amylase. Addition of free calcium is required to 31
- Termamyl-like ⁇ -amylase should have a pH optimum at a pH in the range of 4.5-6.5, preferably in the range of 4.5-5.5.
- variants of the invention may suitably be incorporated in detergent compositions.
- Increased thermostability at low calcium concentrations would be very beneficial for amylase performance in detergents, i.e. the alkaline region.
- Detergent compositions comprising a variant of the invention may additionally comprise one or more other enzymes, such as a lipase, cutinase, protease, cellulase, peroxidase or laccase, and/or another ⁇ -amylase.
- ⁇ -amylase variants of the invention may be incorporated in detergents at conventionally employed concentrations. It is at present contemplated that a variant of the invention may be incorporated in an amount corresponding to 0.00001-1 mg
- the invention also relates to a composition
- a composition comprising 32
- the invention also relates to a composition
- a composition comprising a mixture of one or more variants according the invention derived from (as the parent Termamyl-like ⁇ -amylase) the B . stearothermophilus ⁇ -amylase having the sequence shown in SEQ ID NO: 3 and a hybrid alpha-amylase comprising a part of the B. amyloliquefaciens ⁇ -amylase shown in SEQ ID NO: 5 and a part of the B. licheniformis ⁇ -amylase shown in SEQ ID NO: 4.
- the latter mentioned hydrid Termamyl-like ⁇ -amylase comprises the 445 C-terminal amino acid residues of the B .
- Said latter mentioned hybrid ⁇ -amylase may suitably comprise the following mutations: H156Y+A181T+N190F+A209V+Q264S (using the numbering in SEQ ID NO:
- the composition comprises a mixture of TVB146 and LE174, e.g., in a ratio of 2:1 to 1:2, such as 1:1.
- a ⁇ -amylase variant of the invention or a composition of the invention may in an aspect of the invention be used for washing and/or dishwashing; for textile desizing or for starch liquefaction.
- BSG alpha-amylase B. stearothermophil us alpha-amylase depicted in SEQ ID NO: 3.
- TVB146 alpha-amylase variant B. stearothermophilus alpha- amylase variant depicted in SEQ ID NO: 3 with the following mutations: with the deletion in positions I181-G182 + N193F.
- LE174 hybrid alpha-amylase variant LE174 is a hybrid Termamyl-like alpha-amylase being identical to the Termamyl sequence, i.e., the Bacill us li cheniformis ⁇ - amylase shown in SEQ ID NO: 4, except that the N-terminal 35 amino acid residues (of the mature protein) has been replaced by the N-terminal 33 residues of BAN (mature protein), i.e., the Bacill us amyloliquefaciens alpha-amylase shown in SEQ ID NO: 5, which further havefollowing mutations:
- LE174 was constructed by SOE-PCR (Higuchi et al . 1988, Nucleic Acids Research 16:7351).
- a B. subtilis strain harbouring the relevant expression plasmid is streaked on a LB-agar plate with 10 ⁇ g/ml kanamycin from -80°C stock, and grown overnight at 37°C. The colonies are transferred to 100 ml BPX media supplemented with 10 ⁇ g/ml kanamycin in a 500 ml shaking flask.
- Composition of BPX medium Composition of BPX medium:
- the culture is shaken at 37°C at 270 rpm for 5 days 34
- One Kilo alpah-amylase Unit (1 KNU) is the amount of enzyme which breaks down 5.26 g starch (Merck, Amylum Solubile, Erg. B 6, Batch 9947275) per hour in Novo Nordisk' s standard method for determination of alpha-amylase based upon the following condition:
- This method is used to determine ⁇ -amylase activity in fermentation and recovery samples and formulated and granulated products .
- BS-amylase breaks down the substrate (4, 6-ethylidene (G 7 ) -p- nitrophenyl (G - ⁇ , D-maltoheptaoside (written as ethylidene-G 7 - PNP) into, among other things, G 2 -PNP and G 3 -PNP, where G denoted glucose and PNP p-nitrophenol .
- G2-PNP and G3-PNP are broken down by ⁇ -glucosidase, which is added in excess, into glucose and the yellow-coloured p- nitrophenol .
- the colour reaction is monitored in situ and the change in absorbance over time calculated as an expression of the spreed of the reaction and thus of the activity of the enzyme. See the Boehringer Mannheim 1442 309 guidelines for further details.
- Bacillus stearothermophius alpha-amylase (BS-amylase) activity is determined relative to a standard of declared activity and stated in Kilo Novo Units (Stearothermophilus) or KNU(S)).
- a ready-made kit is used in this analysis to determine ⁇ -amylase activity. Note that the reagents specified for the substrate and ⁇ -glucosidase are not used as described in the Boehringer Mannheim guidelines. However, the designations "buffer”, “glass 1", glass la” and Glass 2" are those referred to in those guidelines .
- the relevant standard is diluted to 0.60 KNU(s)/mL as follows. A calculated quantity of standard is weighed out and added to 200 mL volumetric flask, which is filled to around the 2/3 mark with demineralized water. Stabiliser corresponding to 1% of the volume of the flask is added and the flask is filled to the mark with demineralized water.
- a Hamilton Microlab 1000 is used to produce the dilutions shown below. Demineralized water with 1% stabiliser is used as the diluent.
- a Novo Nordisk A/S BS amylase level control is included in all runs using the Cobas Fara. The control is diluted with 1% stabiliser so that the final dilution is within the range of the standard curve. All weights and dilutions are noted on the worklist
- Fermentation samples (not final samples) from production, all fermentation samples from pilot plants and storage stability samples are weighed out and analyzed once only.
- Double determination over 1 run Process samples, final fermentation samples from production, samples from GLP studies and R&D samples are weighed out and analyzed twice.
- Test samples are diluted with demineralized water with 1% stabiliser to approx. 0.037 KNU(S)/mL on the basis of their expected activity. The final dilution is made direct into the sample cup.
- the Cobas Menu Program is used to suggest the weight/dilutions of samples and level control to be used.
- the five standards are placed in the calibration rack at position 1 to 5 (strongest standard at position 5) , and control placed in the same rack at position 10.
- the substrate is transferred to a 30 mL reagent container and placed in that reagent rack at position 2 (holder 1) .
- the ⁇ -glucosidase help reagent is transferred to a 50 mL reagent container and placed in the reagent rack at position 2 (holder C)
- the activity of the samples is calculated relative to Novo
- Nordisk A/S standard The standard curve is plotted by the analyzer. The curve is to be gently curved, rising steadily to an absorbance of around
- V volume of volumetric flask used in mL
- F dilution factor for second dilution
- W weight of enzyme sample in g
- the mean value is calculated on basis of results lying within the standard curve's activity area.
- Phadebas® tablets as substrate.
- Phadebas tablets (Phadebas® Amylase Test, supplied by Pharmacia Diagnostic) contain a crosslinked insoluble blue-coloured starch polymer which has been mixed with bovine serum albumin and a buffer substance and tabletted.
- 620 nm is a function of the ⁇ -amylase activity.
- the measured 620 nm absorbance after 10 or 15 minutes of incubation is in the range of 0.2 to 2.0 absorbance units at 620 nm. In this absorbance range there is linearity between activity and absorbance (Lambert-Beer law) .
- the dilution of the enzyme must therefore be adjusted to fit this criterion. Under a specified set of conditions (temp., pH, reaction time, buffer conditions) 1 mg of a given ⁇ -amylase will hydrolyse a certain amount of substrate and a blue colour will be produced. The colour intensity is measured at 620 nm.
- the measured absorbance is directly proportional to the specific 41
- This plasmid contains also the gene conferring resistance towards kanamycin and an origin of replication, both obtained from plasmid pUBHO (Gryczan, T.J. et al (1978) J.Bact 134:318-
- BSG variant TVB145 which contains a deletion of 6 nucleotides corresponding to amino acids I181-G182 in the mature protein, is constructed as follows:
- PCR Polymerase Chain Reaction
- DNA primers BSG1 SEQ ID NO: 15
- BSGM2 SEQ ID NO: 18
- BSG1 is identical to a part of the amyS gene whereas BSGM2 contains the 6 bp nucleotide deletion.
- a standard PCR reaction is carried out: 94°C for 5 minutes, 25 cycles of (94°C for 45 seconds, 50°C for 45 seconds, 72°C for 90 seconds), 72°C for 7 minutes using the Pwo polymerase under conditions as recommended by the manufacturer, Boehringer Mannheim Gmbh.
- the resulting approximately 550 bp amplified band was used as a megaprimer (Barik, S and Galinski, MS (1991) : Biotechniques 10: 489-490) together with primer BSG3 in a second PCR with pPL1117 as template resulting in a DNA fragment of approximately 1080 bp.
- This DNA fragment is digested with restriction endonucleases Acc65I and Sail and the resulting approximately 550 bp fragment is ligated into plasmid pPL1117 digested with the same enzymes and transformed into the protease- and amylase-deleted Bacillus subtilis strain SHA273 (described in W092/11357 and WO95/10603) .
- BSG variant TVB146 which contains the same 6 nucleotide deletion as TVB145 and an additional substitution of asparagine 193 for a phenylalanine, N193F, was constructed in a similar way as TVB145 utilizing primer BSGM3 (SEQ ID NO: 19) in the first PCR.
- BSG variant TVB161 containing the deletion of I181-G182, N193F, and L204F, is constructed in a similar way as the two previous variants except that the template for the PCR reactions is plasmid pTVB146 (pPL1117 containing the TVB146- mutations within amyS and the mutagenic oligonucleotide for the first PCR is BSGM3.
- BSG variant TVB162 containing the deletion of I181-G182, N193F, and E210H, is constructed in a similar way as TVB161 except that the mutagenic oligonucleotide is BSGM4 (SEQ ID NO:
- BSG variant TVB163, containing the deletion of I181-G182, N193F, and E214Q, is constructed in a similar way as TVB161 except that the mutagenic oligonucleotide is BSGM5 (SEQ ID NO:
- Method 1 One assay which measures the stability at reduced pH, pH 5.0, in the presence of 5 ppm free calcium.
- Method 2 One assay which measure the stability in the absence of free calcium and where the pH is maintained at pH 6.0. This assay measures the decrease in calcium sensitivity:
- the enzyme was incubated under the relevant conditions (1-4). Samples were taken at 0, 5, 10, 15 and 30 minutes and diluted 25 times (same dilution for all taken samples) in assay buffer (0.1M 50mM Britton buffer pH 7.3) and the activity was measured using the Phadebas assay (Pharmacia) under standard conditions pH 7.3, 37 °C.
- Stability method 1 Low pH stability improvement
- the temperature describet in method 1 has been reduced from 95°C to 70°C since the amylases mentioned for SEQ ID NO: 1 and 2 have a lower thermostability than the one for SEQ ID NO: 3.
- the specific activity was determined using the Phadebas assay (Pharmacia) as activity/mg enzyme.
- the activity was determined using the ⁇ -amylase assay described in the Materials and Methods section herein.
- BSG SEQ ID NO: 3 (Parent enzyme) 20000 NU/mg
- TVB145 SEQ ID NO: 3 with the deletion in positions I181-G182: (Single mutation) 34600 NU/mg
- TVB146 SEQ ID NO: 3 with the deletion in positions I181-G182 + N193F: (Double mutation)
- thermos-flasks After jetting, the liquefied starch was collected and transported in sealed thermos-flasks from the pilot plant to the laboratory, where secondary liquefaction was continued at 95 °C.
- alpha-amylase variant TVB146 performed significantly better under industrially relevant application conditions at low levels of calcium than the parent BSG alpha- amylase.
Abstract
Description
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU94343/98A AU9434398A (en) | 1997-10-13 | 1998-10-13 | Alpha-amylase mutants |
DE69840577T DE69840577D1 (en) | 1997-10-13 | 1998-10-13 | MUTANTS OF alpha-AMYLASE |
JP2000516020A JP4358431B2 (en) | 1997-10-13 | 1998-10-13 | α-Amylase mutant |
EP98947417A EP1023439B1 (en) | 1997-10-13 | 1998-10-13 | alpha-AMYLASE MUTANTS |
CA2305191A CA2305191C (en) | 1997-10-13 | 1998-10-13 | .alpha.-amylase mutants |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK117297 | 1997-10-13 | ||
DK1172/97 | 1997-10-13 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP09152241.7A Previously-Filed-Application EP2206768B1 (en) | 1997-10-13 | 1998-10-13 | Alpha-amylase mutants |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1999019467A1 true WO1999019467A1 (en) | 1999-04-22 |
Family
ID=8101815
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DK1998/000444 WO1999019467A1 (en) | 1997-10-13 | 1998-10-13 | α-AMYLASE MUTANTS |
Country Status (12)
Country | Link |
---|---|
US (1) | US6187576B1 (en) |
EP (3) | EP1023439B1 (en) |
JP (1) | JP4358431B2 (en) |
KR (1) | KR20010015754A (en) |
AR (1) | AR017331A1 (en) |
AT (1) | ATE423192T1 (en) |
AU (1) | AU9434398A (en) |
CA (2) | CA2745783C (en) |
DE (1) | DE69840577D1 (en) |
DK (2) | DK2302027T3 (en) |
ES (3) | ES2436066T3 (en) |
WO (1) | WO1999019467A1 (en) |
Cited By (495)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000060060A2 (en) * | 1999-03-31 | 2000-10-12 | Novozymes A/S | Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same |
WO2001066712A2 (en) | 2000-03-08 | 2001-09-13 | Novozymes A/S | Variants with altered properties |
WO2002010355A2 (en) * | 2000-08-01 | 2002-02-07 | Novozymes A/S | Alpha-amylase mutants with altered stability |
WO2002038787A2 (en) * | 2000-11-10 | 2002-05-16 | Novozymes A/S | Secondary liquefaction of starch in ethanol production |
WO2002092797A2 (en) | 2001-05-15 | 2002-11-21 | Novozymes A/S | Alpha-amylase variant with altered properties |
DE10036752C2 (en) * | 2000-07-28 | 2003-02-20 | Henkel Kgaa | Detergent and cleaning agent with a new amylolytic enzyme from Bacillus sp. A 7-7 (DSM 12368) |
WO2004080923A2 (en) | 2003-03-10 | 2004-09-23 | Novozymes A/S | Alcohol product processes |
WO2004106533A1 (en) | 2003-05-30 | 2004-12-09 | Novozymes A/S | Alcohol product processes |
WO2004113551A1 (en) | 2003-06-25 | 2004-12-29 | Novozymes A/S | Process for the hydrolysis of starch |
WO2005003311A2 (en) | 2003-06-25 | 2005-01-13 | Novozymes A/S | Enzymes for starch processing |
WO2005005646A2 (en) | 2003-06-10 | 2005-01-20 | Novozymes North America, Inc. | Fermentation processes and compositions |
WO2005111203A2 (en) * | 2004-04-08 | 2005-11-24 | Genencor International, Inc. | MUTANT α ΑMYLASES |
WO2006002643A2 (en) | 2004-07-05 | 2006-01-12 | Novozymes A/S | Alpha-amylase variants with altered properties |
WO2006069289A2 (en) | 2004-12-22 | 2006-06-29 | Novozymes North America, Inc | Polypeptides having glucoamylase activity and polynucleotides encoding same |
EP1676913A2 (en) * | 1998-11-16 | 2006-07-05 | Novozymes A/S | Alpha-amylase variants |
WO2007028088A2 (en) | 2005-09-02 | 2007-03-08 | Novozymes North America, Inc. | Methods for enhancing the dewaterability of sludge with alpha-amylase treatment |
WO2007035730A2 (en) | 2005-09-20 | 2007-03-29 | Novozymes North America, Inc. | Process of producing a fermentation product |
WO2007076388A2 (en) | 2005-12-22 | 2007-07-05 | Novozymes North America, Inc. | Processes for producing a fermentation product |
WO2007109750A2 (en) | 2006-03-22 | 2007-09-27 | Novozymes North America, Inc. | Fermentation processes |
WO2007113292A2 (en) | 2006-04-04 | 2007-10-11 | Novozymes A/S | Mashing process |
WO2008134259A1 (en) | 2007-04-24 | 2008-11-06 | Novozymes North America, Inc. | Detoxifying pre-treated lignocellulose-containing materials |
WO2008153815A2 (en) * | 2007-05-30 | 2008-12-18 | Danisco Us, Inc., Genencor Division | Variants of an alpha-amylase with improved production levels in fermentation processes |
WO2009030713A1 (en) | 2007-09-03 | 2009-03-12 | Novozymes A/S | Detoxifying and recycling of washing solution used in pretreatment of lignocellulose-containing materials |
DE10362172B4 (en) * | 2003-03-05 | 2009-04-02 | Henkel Ag & Co. Kgaa | Detergents and cleaners with alpha-amylase variants with improved alkalinity |
US7601527B2 (en) | 1998-11-16 | 2009-10-13 | Novozymes A/S | α-amylase variants |
WO2010008841A2 (en) | 2008-06-23 | 2010-01-21 | Novozymes A/S | Processes for producing fermentation products |
WO2010039812A2 (en) | 2008-09-30 | 2010-04-08 | Novozymes North America, Inc. | Improvement of enzymatic hydrolysis of pre-treated lignocellulose-containing material with distillers dried grains |
WO2010043538A2 (en) | 2008-10-15 | 2010-04-22 | Novozymes A/S | Brewing process |
US7713723B1 (en) | 2000-08-01 | 2010-05-11 | Novozymes A/S | Alpha-amylase mutants with altered properties |
WO2010059413A2 (en) | 2008-11-20 | 2010-05-27 | Novozymes, Inc. | Polypeptides having amylolytic enhancing activity and polynucleotides encoding same |
WO2010078391A2 (en) | 2008-12-30 | 2010-07-08 | Novozymes North America, Inc. | Improvement of enzymatic hydrolysis of pretreated lignocellulose-containing material with dissolved air flotation sludge |
WO2010078392A2 (en) | 2008-12-31 | 2010-07-08 | Novozymes North America, Inc. | Processes of producing fermentation products |
EP2213732A1 (en) | 2003-10-28 | 2010-08-04 | Novozymes North America, Inc. | Hybrid glucoamylases |
WO2010104675A1 (en) * | 2009-03-10 | 2010-09-16 | Danisco Us Inc. | Bacillus megaterium strain dsm90-related alpha-amylases, and methods of use, thereof |
WO2010115021A2 (en) | 2009-04-01 | 2010-10-07 | Danisco Us Inc. | Compositions and methods comprising alpha-amylase variants with altered properties |
EP2258837A1 (en) | 2004-09-10 | 2010-12-08 | Novozymes North America, Inc. | Methods for preventing, removing, reducing, or disrupting biofilm |
WO2011003940A1 (en) | 2009-07-07 | 2011-01-13 | Novozymes A/S | Process for treating a substrate with an enzyme |
WO2011008785A2 (en) | 2009-07-17 | 2011-01-20 | Novozymes A/S | A method of analyzing cellulose decay in cellulosic material hydrolysis |
WO2011017093A1 (en) | 2009-08-07 | 2011-02-10 | Danisco Us Inc. | Alpha-amylase blend for starch processing and method of use thereof |
WO2011020910A1 (en) | 2009-08-21 | 2011-02-24 | Novozymes A/S | Polypeptides having isoamylase activity and polynucleotides encoding same |
EP2295545A1 (en) | 2002-09-26 | 2011-03-16 | Novozymes North America, Inc. | Fermentation methods and compositions |
WO2011066560A1 (en) | 2009-11-30 | 2011-06-03 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
WO2011066576A1 (en) | 2009-11-30 | 2011-06-03 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
WO2011068803A1 (en) | 2009-12-01 | 2011-06-09 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
US7968318B2 (en) | 2006-06-06 | 2011-06-28 | Genencor International, Inc. | Process for conversion of granular starch to ethanol |
WO2011082429A1 (en) | 2010-01-04 | 2011-07-07 | Novozymes A/S | Alpha-amylases |
WO2011087836A2 (en) | 2009-12-22 | 2011-07-21 | Novozymes A/S | Pullulanase variants and uses thereof |
WO2011092136A1 (en) | 2010-01-29 | 2011-08-04 | Novozymes A/S | Biogas production process with enzymatic pre-treatment |
WO2011100161A1 (en) | 2010-02-09 | 2011-08-18 | Novozymes North America, Inc. | Addition of alpha - glucosidase and cobalt for producing fermentation products from starch |
US8017371B2 (en) | 2004-08-02 | 2011-09-13 | Novozymes A/S | Hybrid polypeptide of a maltogenic alpha-amylase and a cyclodextrin glucanotransferase |
US8017351B2 (en) | 2005-06-24 | 2011-09-13 | Novozymes A/S | Amylases for pharmaceutical use |
WO2011123505A1 (en) | 2010-03-30 | 2011-10-06 | Novozymes North America, Inc. | Processes of producing a fermentation product |
WO2011127820A1 (en) | 2010-04-14 | 2011-10-20 | Novozymes A/S | Processes for producing fermentation products |
US8080401B2 (en) | 2004-10-01 | 2011-12-20 | Henkel Ag & Co. Kgaa | Alpha-amylase variants having an elevated solvent stability, method for the production thereof and detergents and cleansers containing these alpha-amylase variants |
US8084240B2 (en) | 2008-06-06 | 2011-12-27 | Danisco Us Inc. | Geobacillus stearothermophilus α-amylase (AmyS) variants with improved properties |
WO2012018775A1 (en) | 2010-08-02 | 2012-02-09 | Novozymes North America, Inc. | Process of producing a fermentation product |
US8153412B2 (en) | 2007-11-05 | 2012-04-10 | Danisco Us Inc. | Variants of Bacillus sp. TS-23 alpha-amylase with altered properties |
WO2012064351A1 (en) | 2010-11-08 | 2012-05-18 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
WO2012064350A1 (en) | 2010-11-08 | 2012-05-18 | Novozymes A/S | Polypeptides Having Glucoamylase Activity and Polynucleotides Encoding Same |
WO2012068047A2 (en) | 2010-11-19 | 2012-05-24 | Novozymes North America, Inc. | Processes of producing a fermentation product |
US8206966B2 (en) | 2007-11-05 | 2012-06-26 | Danisco Us Inc. | Alpha-amylase variants with altered properties |
WO2012088303A2 (en) | 2010-12-22 | 2012-06-28 | Novozymes North America, Inc. | Processes for producing fermentation products |
WO2012093041A1 (en) | 2011-01-04 | 2012-07-12 | Novozymes A/S | Process for producing biogas from pectin and lignocellulose containing material |
US8236545B2 (en) | 2008-02-04 | 2012-08-07 | Danisco Us Inc., Genencor Division | TS23 alpha-amylase variants with altered properties |
WO2012109119A2 (en) | 2011-02-07 | 2012-08-16 | Novozymes North America, Inc. | Process of producing a fermentation product |
WO2012140075A2 (en) | 2011-04-15 | 2012-10-18 | Novozymes A/S | Method for production of brewers wort |
WO2012149275A1 (en) | 2011-04-29 | 2012-11-01 | Danisco Us Inc. | Use of cellulase and glucoamylase to improve ethanol yields from fermentation |
WO2013000945A1 (en) | 2011-06-28 | 2013-01-03 | Novozymes A/S | Biogas from enzyme-treated bagasse |
WO2013006756A2 (en) | 2011-07-06 | 2013-01-10 | Novozymes A/S | Alpha amylase variants and polynucleotides encoding same |
WO2013016115A1 (en) | 2011-07-22 | 2013-01-31 | Novozymes North America, Inc. | Processes for pretreating cellulosic material and improving hydrolysis thereof |
WO2013029496A1 (en) | 2011-08-26 | 2013-03-07 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
WO2013036526A1 (en) | 2011-09-06 | 2013-03-14 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
US8399224B2 (en) | 2007-03-14 | 2013-03-19 | Danisco Us Inc. | Production of ethanol from barley and DDGS containing reduced beta-glucan and phytic acid |
WO2013055676A1 (en) | 2011-10-11 | 2013-04-18 | Novozymes North America, Inc. | Processes for producing fermentation products |
WO2013053801A1 (en) | 2011-10-11 | 2013-04-18 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
WO2013057141A2 (en) | 2011-10-17 | 2013-04-25 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2013057143A2 (en) | 2011-10-17 | 2013-04-25 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2013082486A1 (en) | 2011-12-02 | 2013-06-06 | Novozymes A/S | Processes for producing fermentation products |
WO2013083801A2 (en) | 2011-12-09 | 2013-06-13 | Novozymes A/S | Biogas from substrates comprising animal manure and enzymes |
WO2013096652A1 (en) | 2011-12-21 | 2013-06-27 | Novozymes, Inc. | Methods for determining the degradation of a biomass material |
WO2013110766A1 (en) | 2012-01-26 | 2013-08-01 | Novozymes A/S | Use of polypeptides having protease activity in animal feed and detergents |
US8507243B2 (en) | 2008-09-25 | 2013-08-13 | Danisco Us Inc. | Alpha-amylase blends and methods for using said blends |
WO2013148993A1 (en) | 2012-03-30 | 2013-10-03 | Novozymes North America, Inc. | Processes of producing fermentation products |
WO2013167573A1 (en) | 2012-05-11 | 2013-11-14 | Novozymes A/S | A brewing method |
WO2013167581A1 (en) | 2012-05-07 | 2013-11-14 | Novozymes A/S | Polypeptides having xanthan degrading activity and polynucleotides encoding same |
WO2013171241A1 (en) | 2012-05-16 | 2013-11-21 | Novozymes A/S | Compositions comprising lipase and methods of use thereof |
WO2013189972A2 (en) | 2012-06-20 | 2013-12-27 | Novozymes A/S | Use of polypeptides having protease activity in animal feed and detergents |
WO2014006140A1 (en) | 2012-07-06 | 2014-01-09 | Novozymes A/S | Liquid enzyme composition and method for enzyme recovery |
WO2014027062A1 (en) | 2012-08-17 | 2014-02-20 | Novozymes A/S | Thermostable asparaginase variants and polynucleotides encoding same |
WO2014029821A1 (en) | 2012-08-22 | 2014-02-27 | Novozymes A/S | Metalloproteases from alicyclobacillus sp. |
WO2014029819A1 (en) | 2012-08-22 | 2014-02-27 | Novozymes A/S | Metalloprotease from exiguobacterium |
WO2014060474A1 (en) | 2012-10-17 | 2014-04-24 | Novozymes A/S | Method for production of brewers wort |
WO2014087011A1 (en) | 2012-12-07 | 2014-06-12 | Novozymes A/S | Preventing adhesion of bacteria |
WO2014092960A1 (en) | 2012-12-11 | 2014-06-19 | Danisco Us Inc. | Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof |
WO2014096259A1 (en) | 2012-12-21 | 2014-06-26 | Novozymes A/S | Polypeptides having protease activiy and polynucleotides encoding same |
US8809031B2 (en) | 2007-03-23 | 2014-08-19 | Danisco Us Inc. | Enhanced amylase production by N-terminal addition to mature amylase protein |
WO2014177541A2 (en) | 2013-04-30 | 2014-11-06 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
WO2014183921A1 (en) | 2013-05-17 | 2014-11-20 | Novozymes A/S | Polypeptides having alpha amylase activity |
WO2014184164A1 (en) | 2013-05-14 | 2014-11-20 | Novozymes A/S | Detergent compositions |
WO2014194032A1 (en) | 2013-05-29 | 2014-12-04 | Danisco Us Inc. | Novel metalloproteases |
WO2014194054A1 (en) | 2013-05-29 | 2014-12-04 | Danisco Us Inc. | Novel metalloproteases |
WO2014194117A2 (en) | 2013-05-29 | 2014-12-04 | Danisco Us Inc. | Novel metalloproteases |
WO2014194034A2 (en) | 2013-05-29 | 2014-12-04 | Danisco Us Inc. | Novel metalloproteases |
WO2014206960A1 (en) | 2013-06-26 | 2014-12-31 | Novozymes A/S | Process for manufacturing a feed composition |
WO2014207224A1 (en) | 2013-06-27 | 2014-12-31 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
WO2014207227A1 (en) | 2013-06-27 | 2014-12-31 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
WO2014209800A1 (en) | 2013-06-24 | 2014-12-31 | Novozymes A/S | Processes for recovering oil from fermentation product processes and processes for producing fermentation products |
WO2015001017A2 (en) | 2013-07-04 | 2015-01-08 | Novozymes A/S | Polypeptides having anti-redeposition effect and polynucleotides encoding same |
WO2015007639A1 (en) | 2013-07-17 | 2015-01-22 | Novozymes A/S | Pullulanase chimeras and polynucleotides encoding same |
WO2015035914A1 (en) | 2013-09-11 | 2015-03-19 | Novozymes A/S | Processes for producing fermentation products |
WO2015049370A1 (en) | 2013-10-03 | 2015-04-09 | Novozymes A/S | Detergent composition and use of detergent composition |
WO2015057517A1 (en) | 2013-10-17 | 2015-04-23 | Danisco Us Inc. | Use of hemicellulases to improve ethanol production |
EP2500423B1 (en) | 2003-02-26 | 2015-06-17 | Danisco US Inc. | Amylases producing an altered immunogenic response and methods of making and using the same |
WO2015089447A1 (en) | 2013-12-13 | 2015-06-18 | Danisco Us Inc. | Serine proteases of the bacillus gibsonii-clade |
WO2015089441A1 (en) | 2013-12-13 | 2015-06-18 | Danisco Us Inc. | Serine proteases of bacillus species |
WO2015095358A1 (en) | 2013-12-18 | 2015-06-25 | E. I. Du Pont De Nemours And Company | Cationic poly alpha-1,3-glucan ethers |
WO2015091959A3 (en) * | 2013-12-20 | 2015-08-13 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2015123323A1 (en) | 2014-02-14 | 2015-08-20 | E. I. Du Pont De Nemours And Company | Poly-alpha-1,3-1,6-glucans for viscosity modification |
WO2015134737A1 (en) | 2014-03-05 | 2015-09-11 | Novozymes A/S | Compositions and methods for improving properties of cellulosic textile materials with xyloglucan endotransglycosylase |
WO2015134729A1 (en) | 2014-03-05 | 2015-09-11 | Novozymes A/S | Compositions and methods for improving properties of non-cellulosic textile materials with xyloglucan endotransglycosylase |
WO2015138283A1 (en) | 2014-03-11 | 2015-09-17 | E. I. Du Pont De Nemours And Company | Oxidized poly alpha-1,3-glucan as detergent builder |
WO2015143144A1 (en) | 2014-03-19 | 2015-09-24 | Novozymes A/S | Method for enhancing activity of an x143 polypeptide |
WO2015150457A1 (en) | 2014-04-01 | 2015-10-08 | Novozymes A/S | Polypeptides having alpha amylase activity |
WO2015181119A2 (en) | 2014-05-27 | 2015-12-03 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
WO2015189371A1 (en) | 2014-06-12 | 2015-12-17 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2015195777A1 (en) | 2014-06-19 | 2015-12-23 | E. I. Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
WO2015195960A1 (en) | 2014-06-19 | 2015-12-23 | E. I. Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
WO2015200659A1 (en) | 2014-06-25 | 2015-12-30 | Novozymes A/S | Xylanase variants and polynucleotides encoding same |
WO2016001319A1 (en) | 2014-07-03 | 2016-01-07 | Novozymes A/S | Improved stabilization of non-protease enzyme |
WO2016061438A1 (en) | 2014-10-17 | 2016-04-21 | Danisco Us Inc. | Serine proteases of bacillus species |
WO2016062875A2 (en) | 2014-10-23 | 2016-04-28 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
WO2016069569A2 (en) | 2014-10-27 | 2016-05-06 | Danisco Us Inc. | Serine proteases |
WO2016069548A2 (en) | 2014-10-27 | 2016-05-06 | Danisco Us Inc. | Serine proteases |
WO2016069544A1 (en) | 2014-10-27 | 2016-05-06 | Danisco Us Inc. | Serine proteases |
WO2016069557A1 (en) | 2014-10-27 | 2016-05-06 | Danisco Us Inc. | Serine proteases of bacillus species |
WO2016069552A1 (en) | 2014-10-27 | 2016-05-06 | Danisco Us Inc. | Serine proteases |
WO2016079110A2 (en) | 2014-11-19 | 2016-05-26 | Novozymes A/S | Use of enzyme for cleaning |
WO2016079305A1 (en) | 2014-11-20 | 2016-05-26 | Novozymes A/S | Alicyclobacillus variants and polynucleotides encoding same |
WO2016087327A1 (en) | 2014-12-01 | 2016-06-09 | Novozymes A/S | Polypeptides having pullulanase activity comprising the x25, x45 and cbm41 domains |
WO2016087401A1 (en) | 2014-12-05 | 2016-06-09 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
EP3034588A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Detergent composition |
EP3034589A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Detergent composition |
EP3034591A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Method of automatic dishwashing |
EP3034590A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Method of automatic dishwashing |
EP3034596A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Detergent composition |
EP3034592A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Method of automatic dishwashing |
EP3034597A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Detergent composition |
WO2016096996A1 (en) | 2014-12-16 | 2016-06-23 | Novozymes A/S | Polypeptides having n-acetyl glucosamine oxidase activity |
WO2016106011A1 (en) | 2014-12-23 | 2016-06-30 | E. I. Du Pont De Nemours And Company | Enzymatically produced cellulose |
WO2016135351A1 (en) | 2015-06-30 | 2016-09-01 | Novozymes A/S | Laundry detergent composition, method for washing and use of composition |
US9434932B2 (en) | 2011-06-30 | 2016-09-06 | Novozymes A/S | Alpha-amylase variants |
WO2016162556A1 (en) | 2015-04-10 | 2016-10-13 | Novozymes A/S | Laundry method, use of dnase and detergent composition |
WO2016162558A1 (en) | 2015-04-10 | 2016-10-13 | Novozymes A/S | Detergent composition |
WO2016184944A1 (en) | 2015-05-19 | 2016-11-24 | Novozymes A/S | Odor reduction |
EP3101108A1 (en) | 2015-06-04 | 2016-12-07 | The Procter and Gamble Company | Hand dishwashing liquid detergent composition |
EP3101109A1 (en) | 2015-06-04 | 2016-12-07 | The Procter and Gamble Company | Hand dishwashing liquid detergent composition |
WO2016196202A1 (en) | 2015-05-29 | 2016-12-08 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
WO2016201040A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc. | Water-triggered enzyme suspension |
WO2016201069A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc | Low-density enzyme-containing particles |
WO2016201044A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc | Osmotic burst encapsulates |
EP3106508A1 (en) | 2015-06-18 | 2016-12-21 | Henkel AG & Co. KGaA | Detergent composition comprising subtilase variants |
WO2016202739A1 (en) | 2015-06-16 | 2016-12-22 | Novozymes A/S | Polypeptides with lipase activity and polynucleotides encoding same |
WO2016205755A1 (en) | 2015-06-17 | 2016-12-22 | Danisco Us Inc. | Bacillus gibsonii-clade serine proteases |
WO2016202785A1 (en) | 2015-06-17 | 2016-12-22 | Novozymes A/S | Container |
WO2016205127A1 (en) | 2015-06-18 | 2016-12-22 | Novozymes A/S | Polypeptides having trehalase activity and the use thereof in process of producing fermentation products |
WO2017015329A1 (en) | 2015-07-23 | 2017-01-26 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2017046232A1 (en) | 2015-09-17 | 2017-03-23 | Henkel Ag & Co. Kgaa | Detergent compositions comprising polypeptides having xanthan degrading activity |
WO2017046260A1 (en) | 2015-09-17 | 2017-03-23 | Novozymes A/S | Polypeptides having xanthan degrading activity and polynucleotides encoding same |
US9617527B2 (en) | 2010-04-14 | 2017-04-11 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
WO2017060505A1 (en) | 2015-10-07 | 2017-04-13 | Novozymes A/S | Polypeptides |
WO2017064253A1 (en) | 2015-10-14 | 2017-04-20 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
WO2017066255A1 (en) | 2015-10-14 | 2017-04-20 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
WO2017064269A1 (en) | 2015-10-14 | 2017-04-20 | Novozymes A/S | Polypeptide variants |
WO2017066510A1 (en) | 2015-10-14 | 2017-04-20 | Novozymes A/S | Cleaning of water filtration membranes |
WO2017079751A1 (en) | 2015-11-05 | 2017-05-11 | Danisco Us Inc | Paenibacillus sp. mannanases |
WO2017079756A1 (en) | 2015-11-05 | 2017-05-11 | Danisco Us Inc | Paenibacillus and bacillus spp. mannanases |
WO2017083228A1 (en) | 2015-11-13 | 2017-05-18 | E. I. Du Pont De Nemours And Company | Glucan fiber compositions for use in laundry care and fabric care |
WO2017083226A1 (en) | 2015-11-13 | 2017-05-18 | E. I. Du Pont De Nemours And Company | Glucan fiber compositions for use in laundry care and fabric care |
WO2017083229A1 (en) | 2015-11-13 | 2017-05-18 | E. I. Du Pont De Nemours And Company | Glucan fiber compositions for use in laundry care and fabric care |
WO2017089366A1 (en) | 2015-11-24 | 2017-06-01 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
WO2017093318A1 (en) | 2015-12-01 | 2017-06-08 | Novozymes A/S | Methods for producing lipases |
WO2017106676A1 (en) | 2015-12-18 | 2017-06-22 | Danisco Us Inc | Polypeptides with endoglucanase activity and uses thereof |
WO2017112540A1 (en) | 2015-12-22 | 2017-06-29 | Novozymes A/S | Processes for producing fermentation products |
WO2017117089A1 (en) | 2015-12-28 | 2017-07-06 | Novozymes Bioag A/S | Heat priming of bacterial spores |
WO2017129754A1 (en) | 2016-01-29 | 2017-08-03 | Novozymes A/S | Beta-glucanase variants and polynucleotides encoding same |
WO2017174769A2 (en) | 2016-04-08 | 2017-10-12 | Novozymes A/S | Detergent compositions and uses of the same |
WO2017186943A1 (en) | 2016-04-29 | 2017-11-02 | Novozymes A/S | Detergent compositions and uses thereof |
WO2017192692A1 (en) | 2016-05-03 | 2017-11-09 | Danisco Us Inc | Protease variants and uses thereof |
WO2017192300A1 (en) | 2016-05-05 | 2017-11-09 | Danisco Us Inc | Protease variants and uses thereof |
WO2017191160A1 (en) | 2016-05-03 | 2017-11-09 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding the same |
WO2017194487A1 (en) | 2016-05-09 | 2017-11-16 | Novozymes A/S | Variant polypeptides with improved performance and use of the same |
WO2017210188A1 (en) | 2016-05-31 | 2017-12-07 | Novozymes A/S | Stabilized liquid peroxide compositions |
WO2017207762A1 (en) | 2016-06-03 | 2017-12-07 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
WO2017210295A1 (en) | 2016-05-31 | 2017-12-07 | Danisco Us Inc. | Protease variants and uses thereof |
EP3257931A1 (en) | 2016-06-17 | 2017-12-20 | The Procter and Gamble Company | Detergent composition |
WO2017219011A1 (en) | 2016-06-17 | 2017-12-21 | Danisco Us Inc | Protease variants and uses thereof |
WO2017220422A1 (en) | 2016-06-23 | 2017-12-28 | Novozymes A/S | Use of enzymes, composition and method for removing soil |
WO2018002261A1 (en) | 2016-07-01 | 2018-01-04 | Novozymes A/S | Detergent compositions |
WO2018007573A1 (en) | 2016-07-08 | 2018-01-11 | Novozymes A/S | Detergent compositions with galactanase |
WO2018007435A1 (en) | 2016-07-05 | 2018-01-11 | Novozymes A/S | Pectate lyase variants and polynucleotides encoding same |
WO2018011277A1 (en) | 2016-07-13 | 2018-01-18 | Novozymes A/S | Bacillus cibi dnase variants |
EP3272767A1 (en) | 2016-07-21 | 2018-01-24 | Fornia BioSolutions, Inc. | G24 glucoamylase compositions and methods |
WO2018015303A1 (en) | 2016-07-21 | 2018-01-25 | Novozymes A/S | Serine protease variants and polynucleotides encoding same |
WO2018017105A1 (en) | 2016-07-21 | 2018-01-25 | Fornia Biosolutions, Inc. | G24 glucoamylase compositions and methods |
WO2018015304A1 (en) | 2016-07-21 | 2018-01-25 | Novozymes A/S | Serine protease variants and polynucleotides encoding same |
WO2018015295A1 (en) | 2016-07-18 | 2018-01-25 | Novozymes A/S | Lipase variants, polynucleotides encoding same and the use thereof |
WO2018026384A1 (en) | 2016-08-05 | 2018-02-08 | Fornia Biosolutions, Inc. | 2g16 glucoamylase compositions |
EP3284805A1 (en) | 2016-08-17 | 2018-02-21 | The Procter & Gamble Company | Cleaning composition comprising enzymes |
WO2018037061A1 (en) | 2016-08-24 | 2018-03-01 | Novozymes A/S | Xanthan lyase variants and polynucleotides encoding same |
WO2018037062A1 (en) | 2016-08-24 | 2018-03-01 | Novozymes A/S | Gh9 endoglucanase variants and polynucleotides encoding same |
WO2018037065A1 (en) | 2016-08-24 | 2018-03-01 | Henkel Ag & Co. Kgaa | Detergent composition comprising gh9 endoglucanase variants i |
WO2018037064A1 (en) | 2016-08-24 | 2018-03-01 | Henkel Ag & Co. Kgaa | Detergent compositions comprising xanthan lyase variants i |
WO2018060475A1 (en) | 2016-09-29 | 2018-04-05 | Novozymes A/S | Spore containing granule |
WO2018060216A1 (en) | 2016-09-29 | 2018-04-05 | Novozymes A/S | Use of enzyme for washing, method for washing and warewashing composition |
EP3309249A1 (en) | 2013-07-29 | 2018-04-18 | Novozymes A/S | Protease variants and polynucleotides encoding same |
WO2018075430A1 (en) | 2016-10-17 | 2018-04-26 | Novozymes A/S | Methods of reducing foam during ethanol fermentation |
WO2018077938A1 (en) | 2016-10-25 | 2018-05-03 | Novozymes A/S | Detergent compositions |
WO2018085524A2 (en) | 2016-11-07 | 2018-05-11 | Danisco Us Inc | Laundry detergent composition |
WO2018083093A1 (en) | 2016-11-01 | 2018-05-11 | Novozymes A/S | Multi-core granules |
EP3321360A2 (en) | 2013-01-03 | 2018-05-16 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2018093285A1 (en) | 2016-11-18 | 2018-05-24 | Baltika Breweries - Part Of The Carlsberg Group | Method of producing a grain malt and the malt product obtained in this way |
WO2018098124A1 (en) | 2016-11-23 | 2018-05-31 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
WO2018098381A1 (en) | 2016-11-23 | 2018-05-31 | Novozymes A/S | Improved yeast for ethanol production |
WO2018099762A1 (en) | 2016-12-01 | 2018-06-07 | Basf Se | Stabilization of enzymes in compositions |
WO2018102348A1 (en) * | 2016-11-29 | 2018-06-07 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2018108865A1 (en) | 2016-12-12 | 2018-06-21 | Novozymes A/S | Use of polypeptides |
WO2018118917A1 (en) | 2016-12-21 | 2018-06-28 | Danisco Us Inc. | Protease variants and uses thereof |
WO2018118950A1 (en) | 2016-12-21 | 2018-06-28 | Danisco Us Inc. | Bacillus gibsonii-clade serine proteases |
WO2018156705A1 (en) | 2017-02-24 | 2018-08-30 | Danisco Us Inc. | Compositions and methods for increased protein production in bacillus licheniformis |
EP3372680A1 (en) | 2013-04-30 | 2018-09-12 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
WO2018169750A1 (en) | 2017-03-15 | 2018-09-20 | Danisco Us Inc | Trypsin-like serine proteases and uses thereof |
WO2018178061A1 (en) | 2017-03-31 | 2018-10-04 | Novozymes A/S | Polypeptides having rnase activity |
WO2018177936A1 (en) | 2017-03-31 | 2018-10-04 | Novozymes A/S | Polypeptides having dnase activity |
WO2018184004A1 (en) | 2017-03-31 | 2018-10-04 | Danisco Us Inc | Alpha-amylase combinatorial variants |
WO2018177938A1 (en) | 2017-03-31 | 2018-10-04 | Novozymes A/S | Polypeptides having dnase activity |
WO2018183662A1 (en) | 2017-03-31 | 2018-10-04 | Danisco Us Inc | Delayed release enzyme formulations for bleach-containing detergents |
EP3385361A1 (en) | 2017-04-05 | 2018-10-10 | Henkel AG & Co. KGaA | Detergent compositions comprising bacterial mannanases |
EP3385362A1 (en) | 2017-04-05 | 2018-10-10 | Henkel AG & Co. KGaA | Detergent compositions comprising fungal mannanases |
WO2018184818A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2018185152A1 (en) | 2017-04-04 | 2018-10-11 | Novozymes A/S | Polypeptide compositions and uses thereof |
WO2018185285A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2018185181A1 (en) | 2017-04-04 | 2018-10-11 | Novozymes A/S | Glycosyl hydrolases |
WO2018184816A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2018184873A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Detergent compositions and uses thereof |
WO2018184817A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2018185048A1 (en) | 2017-04-03 | 2018-10-11 | Novozymes A/S | Recovery process |
WO2018185267A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2018185150A1 (en) | 2017-04-04 | 2018-10-11 | Novozymes A/S | Polypeptides |
WO2018185280A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2018185269A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2018202846A1 (en) | 2017-05-05 | 2018-11-08 | Novozymes A/S | Compositions comprising lipase and sulfite |
EP3401385A1 (en) | 2017-05-08 | 2018-11-14 | Henkel AG & Co. KGaA | Detergent composition comprising polypeptide comprising carbohydrate-binding domain |
WO2018206535A1 (en) | 2017-05-08 | 2018-11-15 | Novozymes A/S | Carbohydrate-binding domain and polynucleotides encoding the same |
EP3404087A1 (en) * | 2010-02-10 | 2018-11-21 | Novozymes A/S | Alpha-amylase variants with high stability in presence of a chelating agent |
WO2018222990A1 (en) | 2017-06-02 | 2018-12-06 | Novozymes A/S | Improved yeast for ethanol production |
WO2018224544A1 (en) | 2017-06-08 | 2018-12-13 | Novozymes A/S | Compositions comprising polypeptides having cellulase activity and amylase activity, and uses thereof in cleaning and detergent compositions |
EP3415624A1 (en) | 2014-01-22 | 2018-12-19 | Novozymes A/S | Pullulanase variants and polynucleotides encoding same |
WO2019002356A1 (en) | 2017-06-30 | 2019-01-03 | Novozymes A/S | Enzyme slurry composition |
WO2019006077A1 (en) | 2017-06-30 | 2019-01-03 | Danisco Us Inc | Low-agglomeration, enzyme-containing particles |
WO2019005755A1 (en) | 2017-06-28 | 2019-01-03 | Novozymes A/S | Polypeptides having trehalase activity and polynucleotides encoding same |
WO2019030165A1 (en) | 2017-08-08 | 2019-02-14 | Novozymes A/S | Polypeptides having trehalase activity and the use thereof in process of producing fermentation products |
WO2019036721A2 (en) | 2017-08-18 | 2019-02-21 | Danisco Us Inc | Alpha-amylase variants |
WO2019040412A1 (en) | 2017-08-23 | 2019-02-28 | Danisco Us Inc | Methods and compositions for efficient genetic modifications of bacillus licheniformis strains |
WO2019038060A1 (en) | 2017-08-24 | 2019-02-28 | Henkel Ag & Co. Kgaa | Detergent composition comprising xanthan lyase variants ii |
WO2019038057A1 (en) | 2017-08-24 | 2019-02-28 | Novozymes A/S | Xanthan lyase variants and polynucleotides encoding same |
WO2019038059A1 (en) | 2017-08-24 | 2019-02-28 | Henkel Ag & Co. Kgaa | Detergent compositions comprising gh9 endoglucanase variants ii |
WO2019038058A1 (en) | 2017-08-24 | 2019-02-28 | Novozymes A/S | Gh9 endoglucanase variants and polynucleotides encoding same |
WO2019046232A1 (en) | 2017-08-30 | 2019-03-07 | Novozymes A/S | Combined use of an endo-protease of the m35 family and an exo-protease of the s53 family in the fermentation of starch |
EP3453757A1 (en) | 2013-12-20 | 2019-03-13 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
WO2019055455A1 (en) | 2017-09-15 | 2019-03-21 | Novozymes A/S | Enzyme blends and processes for improving the nutritional quality of animal feed |
WO2019055261A1 (en) | 2017-09-13 | 2019-03-21 | Danisco Us Inc | Modified 5'-untranslated region (utr) sequences for increased protein production in bacillus |
WO2019057902A1 (en) | 2017-09-22 | 2019-03-28 | Novozymes A/S | Novel polypeptides |
WO2019057758A1 (en) | 2017-09-20 | 2019-03-28 | Novozymes A/S | Use of enzymes for improving water absorption and/or whiteness |
EP3461881A1 (en) | 2013-05-03 | 2019-04-03 | Novozymes A/S | Microencapsulation of detergent enzymes |
WO2019067390A1 (en) | 2017-09-27 | 2019-04-04 | The Procter & Gamble Company | Detergent compositions comprising lipases |
WO2019063499A1 (en) | 2017-09-27 | 2019-04-04 | Novozymes A/S | Lipase variants and microcapsule compositions comprising such lipase variants |
WO2019070883A1 (en) | 2017-10-04 | 2019-04-11 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
WO2019068715A1 (en) | 2017-10-02 | 2019-04-11 | Novozymes A/S | Polypeptides having mannanase activity and polynucleotides encoding same |
WO2019068713A1 (en) | 2017-10-02 | 2019-04-11 | Novozymes A/S | Polypeptides having mannanase activity and polynucleotides encoding same |
WO2019076833A1 (en) | 2017-10-16 | 2019-04-25 | Novozymes A/S | Low dusting granules |
WO2019076800A1 (en) | 2017-10-16 | 2019-04-25 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2019076834A1 (en) | 2017-10-16 | 2019-04-25 | Novozymes A/S | Low dusting granules |
WO2019081721A1 (en) | 2017-10-27 | 2019-05-02 | Novozymes A/S | Dnase variants |
WO2019081515A1 (en) | 2017-10-24 | 2019-05-02 | Novozymes A/S | Compositions comprising polypeptides having mannanase activity |
DE102017125560A1 (en) | 2017-11-01 | 2019-05-02 | Henkel Ag & Co. Kgaa | CLEANSING COMPOSITIONS CONTAINING DISPERSINE III |
DE102017125559A1 (en) | 2017-11-01 | 2019-05-02 | Henkel Ag & Co. Kgaa | CLEANSING COMPOSITIONS CONTAINING DISPERSINE II |
WO2019084349A1 (en) | 2017-10-27 | 2019-05-02 | The Procter & Gamble Company | Detergent compositions comprising polypeptide variants |
DE102017125558A1 (en) | 2017-11-01 | 2019-05-02 | Henkel Ag & Co. Kgaa | CLEANING COMPOSITIONS CONTAINING DISPERSINE I |
WO2019083831A1 (en) | 2017-10-23 | 2019-05-02 | Novozymes A/S | Processes for reducing lactic acid in a biofuel fermentation system |
WO2019086528A1 (en) | 2017-11-01 | 2019-05-09 | Novozymes A/S | Polypeptides and compositions comprising such polypeptides |
WO2019089898A1 (en) | 2017-11-02 | 2019-05-09 | Danisco Us Inc | Freezing point depressed solid matrix compositions for melt granulation of enzymes |
WO2019086530A1 (en) | 2017-11-01 | 2019-05-09 | Novozymes A/S | Polypeptides and compositions comprising such polypeptides |
WO2019086532A1 (en) | 2017-11-01 | 2019-05-09 | Novozymes A/S | Methods for cleaning medical devices |
WO2019105781A1 (en) | 2017-11-29 | 2019-06-06 | Basf Se | Storage-stable enzyme preparations, their production and use |
WO2019108599A1 (en) | 2017-11-29 | 2019-06-06 | Danisco Us Inc | Subtilisin variants having improved stability |
WO2019110462A1 (en) | 2017-12-04 | 2019-06-13 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
WO2019113413A1 (en) | 2017-12-08 | 2019-06-13 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2019113415A1 (en) | 2017-12-08 | 2019-06-13 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2019125683A1 (en) | 2017-12-21 | 2019-06-27 | Danisco Us Inc | Enzyme-containing, hot-melt granules comprising a thermotolerant desiccant |
WO2019135868A1 (en) | 2018-01-03 | 2019-07-11 | Danisco Us Inc | Mutant and genetically modified bacillus cells and methods thereof for increased protein production |
WO2019148192A1 (en) | 2018-01-29 | 2019-08-01 | Novozymes A/S | Microorganisms with improved nitrogen utilization for ethanol production |
WO2019154954A1 (en) | 2018-02-08 | 2019-08-15 | Novozymes A/S | Lipase variants and compositions thereof |
WO2019154951A1 (en) | 2018-02-08 | 2019-08-15 | Novozymes A/S | Lipases, lipase variants and compositions thereof |
WO2019156670A1 (en) | 2018-02-08 | 2019-08-15 | Danisco Us Inc. | Thermally-resistant wax matrix particles for enzyme encapsulation |
WO2019161227A1 (en) | 2018-02-15 | 2019-08-22 | Novozymes A/S | Improved yeast for ethanol production |
WO2019162000A1 (en) | 2018-02-23 | 2019-08-29 | Henkel Ag & Co. Kgaa | Detergent composition comprising xanthan lyase and endoglucanase variants |
WO2019175240A1 (en) | 2018-03-13 | 2019-09-19 | Novozymes A/S | Microencapsulation using amino sugar oligomers |
WO2019180111A1 (en) | 2018-03-23 | 2019-09-26 | Novozymes A/S | Subtilase variants and compositions comprising same |
EP3550015A1 (en) | 2014-04-10 | 2019-10-09 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2019197318A1 (en) | 2018-04-09 | 2019-10-17 | Novozymes A/S | Polypeptides having alpha-amylase activity and polynucleotides encoding same |
WO2019201783A1 (en) | 2018-04-19 | 2019-10-24 | Novozymes A/S | Stabilized cellulase variants |
WO2019201793A1 (en) | 2018-04-17 | 2019-10-24 | Novozymes A/S | Polypeptides comprising carbohydrate binding activity in detergent compositions and their use in reducing wrinkles in textile or fabric. |
WO2019201785A1 (en) | 2018-04-19 | 2019-10-24 | Novozymes A/S | Stabilized cellulase variants |
US10457924B2 (en) | 2011-10-28 | 2019-10-29 | Danisco Us Inc | Variant maltohexaose-forming alpha-amylase variants |
WO2019206994A1 (en) | 2018-04-26 | 2019-10-31 | Basf Se | Lipase enzymes |
WO2019211143A1 (en) | 2018-05-03 | 2019-11-07 | Basf Se | Amylase enzymes |
EP3569611A1 (en) | 2013-04-23 | 2019-11-20 | Novozymes A/S | Liquid automatic dish washing detergent compositions with stabilised subtilisin |
WO2019231944A2 (en) | 2018-05-31 | 2019-12-05 | Novozymes A/S | Processes for enhancing yeast growth and productivity |
WO2019238761A1 (en) | 2018-06-15 | 2019-12-19 | Basf Se | Water soluble multilayer films containing wash active chemicals and enzymes |
WO2019245705A1 (en) | 2018-06-19 | 2019-12-26 | Danisco Us Inc | Subtilisin variants |
WO2019245704A1 (en) | 2018-06-19 | 2019-12-26 | Danisco Us Inc | Subtilisin variants |
EP3587569A1 (en) | 2014-03-21 | 2020-01-01 | Danisco US Inc. | Serine proteases of bacillus species |
WO2020002604A1 (en) | 2018-06-28 | 2020-01-02 | Novozymes A/S | Detergent compositions and uses thereof |
WO2020002608A1 (en) | 2018-06-29 | 2020-01-02 | Novozymes A/S | Detergent compositions and uses thereof |
WO2020002255A1 (en) | 2018-06-29 | 2020-01-02 | Novozymes A/S | Subtilase variants and compositions comprising same |
WO2020008043A1 (en) | 2018-07-06 | 2020-01-09 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2020007863A1 (en) | 2018-07-02 | 2020-01-09 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2020007875A1 (en) | 2018-07-03 | 2020-01-09 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2020008024A1 (en) | 2018-07-06 | 2020-01-09 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2020014407A1 (en) | 2018-07-11 | 2020-01-16 | Novozymes A/S | Processes for producing fermentation products |
WO2020023411A1 (en) | 2018-07-25 | 2020-01-30 | Novozymes A/S | Enzyme-expressing yeast for ethanol production |
WO2020028443A1 (en) | 2018-07-31 | 2020-02-06 | Danisco Us Inc | Variant alpha-amylases having amino acid substitutions that lower the pka of the general acid |
EP3608403A2 (en) | 2014-12-15 | 2020-02-12 | Henkel AG & Co. KGaA | Detergent composition comprising subtilase variants |
EP3611260A1 (en) | 2013-07-29 | 2020-02-19 | Novozymes A/S | Protease variants and polynucleotides encoding same |
WO2020047215A1 (en) | 2018-08-30 | 2020-03-05 | Danisco Us Inc | Enzyme-containing granules |
WO2020068486A1 (en) | 2018-09-27 | 2020-04-02 | Danisco Us Inc | Compositions for medical instrument cleaning |
WO2020070014A1 (en) | 2018-10-02 | 2020-04-09 | Novozymes A/S | Cleaning composition comprising anionic surfactant and a polypeptide having rnase activity |
WO2020070011A1 (en) | 2018-10-02 | 2020-04-09 | Novozymes A/S | Cleaning composition |
WO2020070009A1 (en) | 2018-10-02 | 2020-04-09 | Novozymes A/S | Endonuclease 1 ribonucleases for cleaning |
WO2020070063A2 (en) | 2018-10-01 | 2020-04-09 | Novozymes A/S | Detergent compositions and uses thereof |
WO2020070209A1 (en) | 2018-10-02 | 2020-04-09 | Novozymes A/S | Cleaning composition |
WO2020069914A1 (en) | 2018-10-05 | 2020-04-09 | Basf Se | Compounds stabilizing amylases in liquids |
WO2020070199A1 (en) | 2018-10-03 | 2020-04-09 | Novozymes A/S | Polypeptides having alpha-mannan degrading activity and polynucleotides encoding same |
WO2020069913A1 (en) | 2018-10-05 | 2020-04-09 | Basf Se | Compounds stabilizing hydrolases in liquids |
WO2020070249A1 (en) | 2018-10-03 | 2020-04-09 | Novozymes A/S | Cleaning compositions |
WO2020069915A1 (en) | 2018-10-05 | 2020-04-09 | Basf Se | Compounds stabilizing hydrolases in liquids |
WO2020077331A2 (en) | 2018-10-12 | 2020-04-16 | Danisco Us Inc | Alpha-amylases with mutations that improve stability in the presence of chelants |
WO2020076697A1 (en) | 2018-10-08 | 2020-04-16 | Novozymes A/S | Enzyme-expressing yeast for ethanol production |
WO2020074545A1 (en) | 2018-10-11 | 2020-04-16 | Novozymes A/S | Cleaning compositions and uses thereof |
EP3647398A1 (en) | 2018-10-31 | 2020-05-06 | Henkel AG & Co. KGaA | Cleaning compositions containing dispersins v |
EP3647397A1 (en) | 2018-10-31 | 2020-05-06 | Henkel AG & Co. KGaA | Cleaning compositions containing dispersins iv |
US10655093B2 (en) | 2015-06-05 | 2020-05-19 | The Procter & Gamble Company | Compacted liquid laundry detergent composition |
WO2020112599A1 (en) | 2018-11-28 | 2020-06-04 | Danisco Us Inc | Subtilisin variants having improved stability |
WO2020114965A1 (en) | 2018-12-03 | 2020-06-11 | Novozymes A/S | LOW pH POWDER DETERGENT COMPOSITION |
WO2020114968A1 (en) | 2018-12-03 | 2020-06-11 | Novozymes A/S | Powder detergent compositions |
US10683474B2 (en) | 2015-06-05 | 2020-06-16 | The Procter & Gamble Company | Compacted liquid laundry detergent composition |
WO2020127775A1 (en) | 2018-12-21 | 2020-06-25 | Novozymes A/S | Detergent pouch comprising metalloproteases |
WO2020131252A1 (en) | 2018-12-21 | 2020-06-25 | Fornia Biosolutions, Inc. | Variant g6p g7p glucoamylase compositions and methods |
WO2020127796A2 (en) | 2018-12-21 | 2020-06-25 | Novozymes A/S | Polypeptides having peptidoglycan degrading activity and polynucleotides encoding same |
EP3677676A1 (en) | 2019-01-03 | 2020-07-08 | Basf Se | Compounds stabilizing amylases in liquids |
US10711225B2 (en) | 2015-06-05 | 2020-07-14 | The Procter & Gamble Company | Compacted liquid laundry detergent composition |
EP3690037A1 (en) | 2014-12-04 | 2020-08-05 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
WO2020160126A1 (en) | 2019-01-31 | 2020-08-06 | Novozymes A/S | Polypeptides having xylanase activity and use thereof for improving the nutritional quality of animal feed |
WO2020169564A1 (en) | 2019-02-20 | 2020-08-27 | Basf Se | Industrial fermentation process for bacillus using defined medium and trace element feed |
WO2020169563A1 (en) | 2019-02-20 | 2020-08-27 | Basf Se | Industrial fermentation process for bacillus using defined medium and magnesium feed |
EP3702452A1 (en) | 2019-03-01 | 2020-09-02 | Novozymes A/S | Detergent compositions comprising two proteases |
WO2020187883A1 (en) | 2019-03-18 | 2020-09-24 | Novozymes A/S | Polypeptides having pullulanase activity suitable for use in liquefaction |
WO2020188095A1 (en) | 2019-03-21 | 2020-09-24 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
EP3715442A1 (en) | 2016-03-23 | 2020-09-30 | Novozymes A/S | Use of polypeptide having dnase activity for treating fabrics |
WO2020193534A2 (en) | 2019-03-25 | 2020-10-01 | Basf Se | Amylase enzymes |
WO2020193535A2 (en) | 2019-03-25 | 2020-10-01 | Basf Se | Amylase enzymes |
WO2020193532A1 (en) | 2019-03-25 | 2020-10-01 | Basf Se | Cleaning composition having amylase enzymes |
WO2020201403A1 (en) | 2019-04-03 | 2020-10-08 | Novozymes A/S | Polypeptides having beta-glucanase activity, polynucleotides encoding same and uses thereof in cleaning and detergent compositions |
WO2020206058A1 (en) | 2019-04-02 | 2020-10-08 | Novozymes A/S | Process for producing a fermentation product |
EP3722406A1 (en) | 2014-04-11 | 2020-10-14 | Novozymes A/S | Detergent composition |
WO2020208056A1 (en) | 2019-04-12 | 2020-10-15 | Novozymes A/S | Stabilized glycoside hydrolase variants |
WO2020207944A1 (en) | 2019-04-10 | 2020-10-15 | Novozymes A/S | Polypeptide variants |
US10808234B2 (en) | 2018-05-04 | 2020-10-20 | Fornia Biosolutions, Inc. | Variant amylase enzyme compositions and methods |
EP3739029A1 (en) | 2014-07-04 | 2020-11-18 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
WO2020229480A1 (en) | 2019-05-14 | 2020-11-19 | Basf Se | Compounds stabilizing hydrolases in liquids |
EP3741848A2 (en) | 2014-12-19 | 2020-11-25 | Novozymes A/S | Protease variants and polynucleotides encoding same |
EP3741849A2 (en) | 2014-12-19 | 2020-11-25 | Novozymes A/S | Protease variants and polynucleotides encoding same |
WO2020242858A1 (en) | 2019-05-24 | 2020-12-03 | Danisco Us Inc | Subtilisin variants and methods of use |
WO2020247582A1 (en) | 2019-06-06 | 2020-12-10 | Danisco Us Inc | Methods and compositions for cleaning |
WO2020249546A1 (en) | 2019-06-13 | 2020-12-17 | Basf Se | Method of recovering a protein from fermentation broth using a divalent cation |
WO2021001400A1 (en) | 2019-07-02 | 2021-01-07 | Novozymes A/S | Lipase variants and compositions thereof |
WO2021004830A1 (en) | 2019-07-05 | 2021-01-14 | Basf Se | Industrial fermentation process for microbial cells using a fed-batch pre-culture |
WO2021009067A1 (en) | 2019-07-12 | 2021-01-21 | Novozymes A/S | Enzymatic emulsions for detergents |
WO2021021458A1 (en) | 2019-07-26 | 2021-02-04 | Novozymes A/S | Microorganisms with improved nitrogen transport for ethanol production |
WO2021026201A1 (en) | 2019-08-05 | 2021-02-11 | Novozymes A/S | Enzyme blends and processes for producing a high protein feed ingredient from a whole stillage byproduct |
WO2021025872A1 (en) | 2019-08-06 | 2021-02-11 | Novozymes A/S | Fusion proteins for improved enzyme expression |
WO2021030267A1 (en) | 2019-08-14 | 2021-02-18 | Danisco Us Inc | Compositions and methods for increased protein production in bacillus licheniformis |
WO2021032881A1 (en) | 2019-08-22 | 2021-02-25 | Basf Se | Amylase variants |
EP3786269A1 (en) | 2013-06-06 | 2021-03-03 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2021037895A1 (en) | 2019-08-27 | 2021-03-04 | Novozymes A/S | Detergent composition |
WO2021037878A1 (en) | 2019-08-27 | 2021-03-04 | Novozymes A/S | Composition comprising a lipase |
WO2021055395A1 (en) | 2019-09-16 | 2021-03-25 | Novozymes A/S | Polypeptides having beta-glucanase activity and polynucleotides encoding same |
WO2021053127A1 (en) | 2019-09-19 | 2021-03-25 | Novozymes A/S | Detergent composition |
WO2021064068A1 (en) | 2019-10-03 | 2021-04-08 | Novozymes A/S | Polypeptides comprising at least two carbohydrate binding domains |
WO2021074430A1 (en) | 2019-10-18 | 2021-04-22 | Basf Se | Storage-stable hydrolase containing liquids |
WO2021080948A2 (en) | 2019-10-24 | 2021-04-29 | Danisco Us Inc | Variant maltopentaose/maltohexaose-forming alpha-amylases |
WO2021096857A1 (en) | 2019-11-11 | 2021-05-20 | Danisco Us Inc | Compositions and methods for enhanced protein production in bacillus cells |
WO2021105336A1 (en) | 2019-11-29 | 2021-06-03 | Basf Se | Compositions comprising polymer and enzyme |
WO2021119304A1 (en) | 2019-12-10 | 2021-06-17 | Novozymes A/S | Microorganism for improved pentose fermentation |
WO2021115912A1 (en) | 2019-12-09 | 2021-06-17 | Basf Se | Formulations comprising a hydrophobically modified polyethyleneimine and one or more enzymes |
WO2021121394A1 (en) | 2019-12-20 | 2021-06-24 | Novozymes A/S | Stabilized liquid boron-free enzyme compositions |
WO2021123307A2 (en) | 2019-12-20 | 2021-06-24 | Novozymes A/S | Polypeptides having proteolytic activity and use thereof |
WO2021122118A1 (en) | 2019-12-20 | 2021-06-24 | Henkel Ag & Co. Kgaa | Cleaning compositions comprising dispersins vi |
WO2021122117A1 (en) | 2019-12-20 | 2021-06-24 | Henkel Ag & Co. Kgaa | Cleaning composition coprising a dispersin and a carbohydrase |
WO2021126966A1 (en) | 2019-12-16 | 2021-06-24 | Novozymes A/S | Processes for producing fermentation products |
WO2021122120A2 (en) | 2019-12-20 | 2021-06-24 | Henkel Ag & Co. Kgaa | Cleaning compositions comprising dispersins viii |
WO2021146411A1 (en) | 2020-01-15 | 2021-07-22 | Danisco Us Inc | Compositions and methods for enhanced protein production in bacillus licheniformis |
WO2021152123A1 (en) | 2020-01-31 | 2021-08-05 | Novozymes A/S | Mannanase variants and polynucleotides encoding same |
WO2021151536A1 (en) | 2020-01-29 | 2021-08-05 | Unilever Ip Holdings B.V. | Laundry detergent product |
WO2021152120A1 (en) | 2020-01-31 | 2021-08-05 | Novozymes A/S | Mannanase variants and polynucleotides encoding same |
WO2021163011A2 (en) | 2020-02-10 | 2021-08-19 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2021163030A2 (en) | 2020-02-10 | 2021-08-19 | Novozymes A/S | Polypeptides having alpha-amylase activity and polynucleotides encoding same |
EP3872175A1 (en) | 2015-06-18 | 2021-09-01 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
EP3872174A1 (en) | 2015-05-13 | 2021-09-01 | Danisco US Inc. | Aprl-clade protease variants and uses thereof |
EP3878957A1 (en) | 2014-05-27 | 2021-09-15 | Novozymes A/S | Methods for producing lipases |
EP3878960A1 (en) | 2014-07-04 | 2021-09-15 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
EP3892708A1 (en) | 2020-04-06 | 2021-10-13 | Henkel AG & Co. KGaA | Cleaning compositions comprising dispersin variants |
WO2021204838A1 (en) | 2020-04-08 | 2021-10-14 | Novozymes A/S | Carbohydrate binding module variants |
WO2021214059A1 (en) | 2020-04-21 | 2021-10-28 | Novozymes A/S | Cleaning compositions comprising polypeptides having fructan degrading activity |
EP2859097B1 (en) | 2012-06-08 | 2021-11-03 | Danisco US Inc. | Alpha-amylase variants derived from the alpha amylase of cytophaga sp. amylasei(cspamy2). |
EP3907271A1 (en) | 2020-05-07 | 2021-11-10 | Novozymes A/S | Cleaning composition, use and method of cleaning |
WO2021239818A1 (en) | 2020-05-26 | 2021-12-02 | Novozymes A/S | Subtilase variants and compositions comprising same |
EP3929285A2 (en) | 2015-07-01 | 2021-12-29 | Novozymes A/S | Methods of reducing odor |
WO2021259099A1 (en) | 2020-06-24 | 2021-12-30 | Novozymes A/S | Use of cellulases for removing dust mite from textile |
EP3936593A1 (en) | 2020-07-08 | 2022-01-12 | Henkel AG & Co. KGaA | Cleaning compositions and uses thereof |
WO2022008732A1 (en) | 2020-07-10 | 2022-01-13 | Basf Se | Enhancing the activity of antimicrobial preservatives |
WO2022023250A1 (en) | 2020-07-27 | 2022-02-03 | Unilever Ip Holdings B.V. | Use of an enzyme and surfactant for inhibiting microorganisms |
EP3950939A2 (en) | 2015-07-06 | 2022-02-09 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
WO2022043547A1 (en) | 2020-08-28 | 2022-03-03 | Novozymes A/S | Protease variants with improved solubility |
WO2022047149A1 (en) | 2020-08-27 | 2022-03-03 | Danisco Us Inc | Enzymes and enzyme compositions for cleaning |
WO2022043321A2 (en) | 2020-08-25 | 2022-03-03 | Novozymes A/S | Variants of a family 44 xyloglucanase |
WO2022058322A1 (en) | 2020-09-15 | 2022-03-24 | Novozymes A/S | Animal feed comprising insects or insect meal |
WO2022063699A1 (en) | 2020-09-22 | 2022-03-31 | Basf Se | Improved combination of protease and protease inhibitor with secondary enzyme |
WO2022063698A1 (en) | 2020-09-22 | 2022-03-31 | Basf Se | Liquid composition comprising peptide aldehyde |
WO2022074037A2 (en) | 2020-10-07 | 2022-04-14 | Novozymes A/S | Alpha-amylase variants |
WO2022084303A2 (en) | 2020-10-20 | 2022-04-28 | Novozymes A/S | Use of polypeptides having dnase activity |
WO2022090320A1 (en) | 2020-10-28 | 2022-05-05 | Novozymes A/S | Use of lipoxygenase |
WO2022090361A2 (en) | 2020-10-29 | 2022-05-05 | Novozymes A/S | Lipase variants and compositions comprising such lipase variants |
WO2022090564A1 (en) | 2020-11-02 | 2022-05-05 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
WO2022103725A1 (en) | 2020-11-13 | 2022-05-19 | Novozymes A/S | Detergent composition comprising a lipase |
WO2022106400A1 (en) | 2020-11-18 | 2022-05-27 | Novozymes A/S | Combination of immunochemically different proteases |
WO2022106404A1 (en) | 2020-11-18 | 2022-05-27 | Novozymes A/S | Combination of proteases |
EP4015629A1 (en) | 2020-12-18 | 2022-06-22 | Basf Se | Polymer mixtures for increasing stability and performance of hydrolase-containing detergents |
EP4032966A1 (en) | 2021-01-22 | 2022-07-27 | Novozymes A/S | Liquid enzyme composition with sulfite scavenger |
WO2022165107A1 (en) | 2021-01-29 | 2022-08-04 | Danisco Us Inc | Compositions for cleaning and methods related thereto |
WO2022162043A1 (en) | 2021-01-28 | 2022-08-04 | Novozymes A/S | Lipase with low malodor generation |
EP4039806A1 (en) | 2021-02-04 | 2022-08-10 | Henkel AG & Co. KGaA | Detergent composition comprising xanthan lyase and endoglucanase variants with im-proved stability |
WO2022171872A1 (en) | 2021-02-12 | 2022-08-18 | Novozymes A/S | Stabilized biological detergents |
WO2022171780A2 (en) | 2021-02-12 | 2022-08-18 | Novozymes A/S | Alpha-amylase variants |
EP4047088A1 (en) | 2021-02-22 | 2022-08-24 | Basf Se | Amylase variants |
WO2022175435A1 (en) | 2021-02-22 | 2022-08-25 | Basf Se | Amylase variants |
WO2022178432A1 (en) | 2021-02-22 | 2022-08-25 | Danisco Us Inc. | Methods and compositions for producing proteins of interest in pigment deficient bacillus cells |
US11441140B2 (en) | 2015-12-07 | 2022-09-13 | Henkel Ag & Co. Kgaa | Dishwashing compositions comprising polypeptides having beta-glucanase activity and uses thereof |
WO2022189521A1 (en) | 2021-03-12 | 2022-09-15 | Novozymes A/S | Polypeptide variants |
EP4060036A1 (en) | 2021-03-15 | 2022-09-21 | Novozymes A/S | Polypeptide variants |
WO2022194673A1 (en) | 2021-03-15 | 2022-09-22 | Novozymes A/S | Dnase variants |
WO2022199418A1 (en) | 2021-03-26 | 2022-09-29 | Novozymes A/S | Detergent composition with reduced polymer content |
WO2022261003A1 (en) | 2021-06-07 | 2022-12-15 | Novozymes A/S | Engineered microorganism for improved ethanol fermentation |
WO2022268885A1 (en) | 2021-06-23 | 2022-12-29 | Novozymes A/S | Alpha-amylase polypeptides |
WO2023278297A1 (en) | 2021-06-30 | 2023-01-05 | Danisco Us Inc | Variant lipases and uses thereof |
WO2023023644A1 (en) | 2021-08-20 | 2023-02-23 | Danisco Us Inc. | Polynucleotides encoding novel nucleases, compositions thereof and methods thereof for eliminating dna from protein preparations |
WO2023034486A2 (en) | 2021-09-03 | 2023-03-09 | Danisco Us Inc. | Laundry compositions for cleaning |
WO2023039270A2 (en) | 2021-09-13 | 2023-03-16 | Danisco Us Inc. | Bioactive-containing granules |
EP4163305A1 (en) | 2013-12-16 | 2023-04-12 | Nutrition & Biosciences USA 4, Inc. | Use of poly alpha-1,3-glucan ethers as viscosity modifiers |
WO2023114988A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Variant maltopentaose/maltohexaose-forming alpha-amylases |
WO2023114932A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Subtilisin variants and methods of use |
WO2023114939A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Subtilisin variants and methods of use |
WO2023114936A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Subtilisin variants and methods of use |
WO2023116569A1 (en) | 2021-12-21 | 2023-06-29 | Novozymes A/S | Composition comprising a lipase and a booster |
WO2023118015A1 (en) | 2021-12-21 | 2023-06-29 | Basf Se | Environmental attributes for care composition ingredients |
EP4206309A1 (en) | 2021-12-30 | 2023-07-05 | Novozymes A/S | Protein particles with improved whiteness |
EP4219702A2 (en) | 2015-12-30 | 2023-08-02 | Novozymes A/S | Enzyme variants and polynucleotides encoding the same |
EP4234664A1 (en) | 2022-02-24 | 2023-08-30 | Evonik Operations GmbH | Composition comprising glucolipids and enzymes |
WO2023168234A1 (en) | 2022-03-01 | 2023-09-07 | Danisco Us Inc. | Enzymes and enzyme compositions for cleaning |
WO2023165507A1 (en) | 2022-03-02 | 2023-09-07 | Novozymes A/S | Use of xyloglucanase for improvement of sustainability of detergents |
WO2023165950A1 (en) | 2022-03-04 | 2023-09-07 | Novozymes A/S | Dnase variants and compositions |
WO2023194204A1 (en) | 2022-04-08 | 2023-10-12 | Novozymes A/S | Hexosaminidase variants and compositions |
WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
WO2023233028A1 (en) | 2022-06-03 | 2023-12-07 | Unilever Ip Holdings B.V. | Laundry detergent product |
WO2023247664A2 (en) | 2022-06-24 | 2023-12-28 | Novozymes A/S | Lipase variants and compositions comprising such lipase variants |
WO2023247348A1 (en) | 2022-06-21 | 2023-12-28 | Novozymes A/S | Mannanase variants and polynucleotides encoding same |
WO2023250301A1 (en) | 2022-06-21 | 2023-12-28 | Danisco Us Inc. | Methods and compositions for cleaning comprising a polypeptide having thermolysin activity |
WO2024012894A1 (en) | 2022-07-15 | 2024-01-18 | Basf Se | Alkanolamine formates for enzyme stabilization in liquid formulations |
WO2024033136A1 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Amylase variants |
WO2024033135A2 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Amylase variants |
US11920170B2 (en) | 2015-12-09 | 2024-03-05 | Danisco Us Inc. | Alpha-amylase combinatorial variants |
WO2024050346A1 (en) | 2022-09-02 | 2024-03-07 | Danisco Us Inc. | Detergent compositions and methods related thereto |
WO2024050343A1 (en) | 2022-09-02 | 2024-03-07 | Danisco Us Inc. | Subtilisin variants and methods related thereto |
WO2024050339A1 (en) | 2022-09-02 | 2024-03-07 | Danisco Us Inc. | Mannanase variants and methods of use |
US11939552B2 (en) | 2013-06-24 | 2024-03-26 | Novozymes A/S | Process of recovering oil |
Families Citing this family (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BRPI9813328B1 (en) * | 1997-10-30 | 2016-04-12 | Novo Nordisk As | alpha-amylase variant, recombinant expression vector, use of an alpha-amylase variant, detergent additive, detergent composition, and manual or automatic laundry composition |
US6623948B1 (en) * | 1999-03-31 | 2003-09-23 | Novozymes A/S | Nucleic acid sequences encoding alkaline alpha-amylases |
HUP0300840A2 (en) * | 2000-07-28 | 2003-07-28 | Henkel Kommanditgesellschaft Auf Aktien | Novel, amylolytic enzyme ectracted from bacillus sp. a 7-7 (dsm 12368) and washing and cleaning agents containing this novel amylolytic enzyme |
US6729019B2 (en) * | 2001-07-11 | 2004-05-04 | Formfactor, Inc. | Method of manufacturing a probe card |
TW546858B (en) * | 2001-07-11 | 2003-08-11 | Formfactor Inc | Method of manufacturing a probe card |
DE10138753B4 (en) * | 2001-08-07 | 2017-07-20 | Henkel Ag & Co. Kgaa | Detergents and cleaners with hybrid alpha-amylases |
WO2006052787A2 (en) * | 2004-11-08 | 2006-05-18 | Novozymes North America, Inc. | Liquefaction of starch-containing material |
WO2006066594A2 (en) * | 2004-12-23 | 2006-06-29 | Novozymes A/S | Alpha-amylase variants |
US7629158B2 (en) | 2006-06-16 | 2009-12-08 | The Procter & Gamble Company | Cleaning and/or treatment compositions |
MX2009009378A (en) | 2007-03-09 | 2009-09-22 | Danisco Us Inc Genencor Div | Alkaliphilic bacillus species a-amylase variants, compositions comprising a-amylase variants, and methods of use. |
CN101896611A (en) * | 2007-10-12 | 2010-11-24 | 诺维信公司 | A process of producing a fermentation product |
WO2009100138A2 (en) * | 2008-02-04 | 2009-08-13 | Danisco Us Inc., Genencor Division | Variants of bacillus stearothermophilus alpha-amylase and uses thereof |
JP2011510682A (en) * | 2008-02-06 | 2011-04-07 | ダニスコ・ユーエス・インク | A pH-free system for the production of fermentable sugars and alcohols |
EP2283136A2 (en) * | 2008-04-30 | 2011-02-16 | Danisco US Inc. | New chimeric alpha-amylase variants |
EP2357220A1 (en) * | 2010-02-10 | 2011-08-17 | The Procter & Gamble Company | Cleaning composition comprising amylase variants with high stability in the presence of a chelating agent |
EP2383329A1 (en) | 2010-04-23 | 2011-11-02 | The Procter & Gamble Company | Particle |
PL2380963T3 (en) | 2010-04-23 | 2016-07-29 | Procter & Gamble | Method of perfuming |
EP2380962B1 (en) | 2010-04-23 | 2016-03-30 | The Procter and Gamble Company | Particle |
ES2533368T3 (en) | 2010-04-23 | 2015-04-09 | The Procter & Gamble Company | Dishwasher product |
PL2380961T3 (en) | 2010-04-23 | 2018-10-31 | The Procter & Gamble Company | Detergent composition |
EP2380478A1 (en) | 2010-04-23 | 2011-10-26 | The Procter & Gamble Company | Automatic dishwashing product |
JP5813753B2 (en) | 2010-05-06 | 2015-11-17 | ザ プロクター アンド ギャンブルカンパニー | Consumer products with protease variants |
WO2012057781A1 (en) | 2010-10-29 | 2012-05-03 | The Procter & Gamble Company | Cleaning and/or treatment compositions comprising a fungal serine protease |
CN107750275A (en) | 2015-05-08 | 2018-03-02 | 诺维信公司 | Alpha-amylase variants and the polynucleotides for encoding them |
JP7175612B2 (en) | 2015-05-08 | 2022-11-21 | ノボザイムス アクティーゼルスカブ | Alpha-Amylase Variants and Polynucleotides Encoding the Variants |
US20210122998A1 (en) * | 2019-10-24 | 2021-04-29 | The Procter & Gamble Company | Automatic dishwashing detergent composition comprising an amylase |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991000353A2 (en) * | 1989-06-29 | 1991-01-10 | Gist-Brocades N.V. | MUTANT MICROBIAL α-AMYLASES WITH INCREASED THERMAL, ACID AND/OR ALKALINE STABILITY |
WO1995010603A1 (en) * | 1993-10-08 | 1995-04-20 | Novo Nordisk A/S | Amylase variants |
WO1995035382A2 (en) * | 1994-06-17 | 1995-12-28 | Genecor International Inc. | NOVEL AMYLOLYTIC ENZYMES DERIVED FROM THE B. LICHENIFORMIS α-AMYLASE, HAVING IMPROVED CHARACTERISTICS |
WO1996023873A1 (en) * | 1995-02-03 | 1996-08-08 | Novo Nordisk A/S | Amylase variants |
WO1997041213A1 (en) * | 1996-04-30 | 1997-11-06 | Novo Nordisk A/S | α-AMYLASE MUTANTS |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3912590A (en) | 1973-01-03 | 1975-10-14 | Novo Industri As | Procedure for liquefying starch |
JPS57174089A (en) | 1981-04-20 | 1982-10-26 | Novo Industri As | Chain dividing enzyme product |
US4760025A (en) | 1984-05-29 | 1988-07-26 | Genencor, Inc. | Modified enzymes and methods for making same |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
DK311186D0 (en) | 1986-06-30 | 1986-06-30 | Novo Industri As | ENZYMES |
FI89076C (en) | 1986-07-09 | 1993-08-10 | Novo Nordisk As | ALFA-AMYLASBLANDNINGAR FOER ATT OEVERFOERA STAERKELSE I VAETSKEFORM OCH FOERFARANDE FOER OEVERFOERING AV STAERKELSE I FLYTANDE FORM |
DE3909096A1 (en) | 1989-03-20 | 1990-09-27 | Garabed Antranikian | ALPHA AMYLASE |
CA2082279C (en) | 1990-05-09 | 2007-09-04 | Grethe Rasmussen | Cellulase preparation comprising an endoglucanase enzyme |
KR100237967B1 (en) | 1990-12-21 | 2000-01-15 | 한센 핀 베네드 | Enzyme mutants having a low degree of variation of the molecular charge over a ph range |
DK0867504T4 (en) | 1993-02-11 | 2011-08-29 | Genencor Int | Oxidatively stable alpha-amylase |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
CA2186592C (en) | 1994-03-29 | 2008-02-19 | Helle Outtrup | Alkaline bacillus amylase |
DE4422198C2 (en) | 1994-06-24 | 1997-08-28 | Audi Ag | Method for controlling the electrical heating of a catalytic converter |
KR100511499B1 (en) | 1995-02-03 | 2005-12-21 | 노보자임스 에이/에스 | A method of designing alpha-amylase mutants with predetermined properties |
ATE267248T1 (en) | 1995-08-11 | 2004-06-15 | Novozymes As | NOVEL LIPOLYTIC ENZYMES |
BRPI9813328B1 (en) * | 1997-10-30 | 2016-04-12 | Novo Nordisk As | alpha-amylase variant, recombinant expression vector, use of an alpha-amylase variant, detergent additive, detergent composition, and manual or automatic laundry composition |
-
1998
- 1998-10-13 DK DK10182050.4T patent/DK2302027T3/en active
- 1998-10-13 KR KR1020007003941A patent/KR20010015754A/en not_active Application Discontinuation
- 1998-10-13 EP EP98947417A patent/EP1023439B1/en not_active Expired - Lifetime
- 1998-10-13 EP EP10182050.4A patent/EP2302027B1/en not_active Expired - Lifetime
- 1998-10-13 CA CA2745783A patent/CA2745783C/en not_active Expired - Lifetime
- 1998-10-13 AU AU94343/98A patent/AU9434398A/en not_active Abandoned
- 1998-10-13 AR ARP980105089A patent/AR017331A1/en active IP Right Grant
- 1998-10-13 EP EP09152241.7A patent/EP2206768B1/en not_active Expired - Lifetime
- 1998-10-13 ES ES10182050T patent/ES2436066T3/en not_active Expired - Lifetime
- 1998-10-13 ES ES09152241.7T patent/ES2536878T3/en not_active Expired - Lifetime
- 1998-10-13 US US09/170,670 patent/US6187576B1/en not_active Expired - Lifetime
- 1998-10-13 AT AT98947417T patent/ATE423192T1/en not_active IP Right Cessation
- 1998-10-13 DE DE69840577T patent/DE69840577D1/en not_active Expired - Lifetime
- 1998-10-13 CA CA2305191A patent/CA2305191C/en not_active Expired - Lifetime
- 1998-10-13 WO PCT/DK1998/000444 patent/WO1999019467A1/en not_active Application Discontinuation
- 1998-10-13 DK DK09152241.7T patent/DK2206768T3/en active
- 1998-10-13 ES ES98947417T patent/ES2322825T3/en not_active Expired - Lifetime
- 1998-10-13 JP JP2000516020A patent/JP4358431B2/en not_active Expired - Lifetime
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1991000353A2 (en) * | 1989-06-29 | 1991-01-10 | Gist-Brocades N.V. | MUTANT MICROBIAL α-AMYLASES WITH INCREASED THERMAL, ACID AND/OR ALKALINE STABILITY |
WO1995010603A1 (en) * | 1993-10-08 | 1995-04-20 | Novo Nordisk A/S | Amylase variants |
WO1995035382A2 (en) * | 1994-06-17 | 1995-12-28 | Genecor International Inc. | NOVEL AMYLOLYTIC ENZYMES DERIVED FROM THE B. LICHENIFORMIS α-AMYLASE, HAVING IMPROVED CHARACTERISTICS |
WO1996023873A1 (en) * | 1995-02-03 | 1996-08-08 | Novo Nordisk A/S | Amylase variants |
WO1997041213A1 (en) * | 1996-04-30 | 1997-11-06 | Novo Nordisk A/S | α-AMYLASE MUTANTS |
Cited By (681)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1676913A2 (en) * | 1998-11-16 | 2006-07-05 | Novozymes A/S | Alpha-amylase variants |
US8465957B2 (en) | 1998-11-16 | 2013-06-18 | Novozymes A/S | Alpha-amylase variants |
EP1676913A3 (en) * | 1998-11-16 | 2007-08-29 | Novozymes A/S | Alpha-amylase variants |
US7601527B2 (en) | 1998-11-16 | 2009-10-13 | Novozymes A/S | α-amylase variants |
WO2000060060A3 (en) * | 1999-03-31 | 2001-04-19 | Novo Nordisk As | Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same |
WO2000060060A2 (en) * | 1999-03-31 | 2000-10-12 | Novozymes A/S | Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same |
EP2011864A1 (en) * | 1999-03-31 | 2009-01-07 | Novozymes A/S | Polypeptides having alkaline alpha-amylase activity and nucleic acids encoding same |
JP2004505606A (en) * | 2000-03-08 | 2004-02-26 | ノボザイムス アクティーゼルスカブ | Variants with altered properties |
WO2001066712A3 (en) * | 2000-03-08 | 2002-04-18 | Novozymes As | Variants with altered properties |
EP2302048A1 (en) | 2000-03-08 | 2011-03-30 | Novozymes A/S | Variants with altered properties |
EP3594334A2 (en) | 2000-03-08 | 2020-01-15 | Novozymes A/S | Variants with altered properties |
EP2221365A1 (en) | 2000-03-08 | 2010-08-25 | Novozymes A/S | Variants with altered properties |
US8883970B2 (en) | 2000-03-08 | 2014-11-11 | Novozymes A/S | Alpha-amylase variants |
EP2298875A2 (en) | 2000-03-08 | 2011-03-23 | Novozymes A/S | Variants with altered properties |
EP2298875A3 (en) * | 2000-03-08 | 2012-01-04 | Novozymes A/S | Variants with altered properties |
US9856437B2 (en) | 2000-03-08 | 2018-01-02 | Novozymes A/S | Amylase variants |
US7432099B2 (en) | 2000-03-08 | 2008-10-07 | Novozymes A/S | Amylase variants |
EP3594334A3 (en) * | 2000-03-08 | 2020-03-18 | Novozymes A/S | Variants with altered properties |
US8609811B2 (en) | 2000-03-08 | 2013-12-17 | Novozymes A/S | Amylase variants |
WO2001066712A2 (en) | 2000-03-08 | 2001-09-13 | Novozymes A/S | Variants with altered properties |
DE10036752C2 (en) * | 2000-07-28 | 2003-02-20 | Henkel Kgaa | Detergent and cleaning agent with a new amylolytic enzyme from Bacillus sp. A 7-7 (DSM 12368) |
US7713723B1 (en) | 2000-08-01 | 2010-05-11 | Novozymes A/S | Alpha-amylase mutants with altered properties |
EP2204446A1 (en) | 2000-08-01 | 2010-07-07 | Novozymes A/S | Alpha-amylase mutants with altered properties |
WO2002010355A2 (en) * | 2000-08-01 | 2002-02-07 | Novozymes A/S | Alpha-amylase mutants with altered stability |
JP4855632B2 (en) * | 2000-08-01 | 2012-01-18 | ノボザイムス アクティーゼルスカブ | Α-Amylase mutants with altered properties |
JP2011224015A (en) * | 2000-08-01 | 2011-11-10 | Novozyme As | α-AMYLASE MUTANT WITH ALTERED PROPERTY |
EP2298903A2 (en) | 2000-08-01 | 2011-03-23 | Novozymes A/S | Alpha-amylase mutants with altered properties |
EP2308979A2 (en) | 2000-08-01 | 2011-04-13 | Novozymes A/S | Alpha-amylase mutants with altered properties |
EP2180035A1 (en) * | 2000-08-01 | 2010-04-28 | Novozymes A/S | Alpha-amylase mutants with altered properties |
WO2002010355A3 (en) * | 2000-08-01 | 2003-09-12 | Novozymes As | Alpha-amylase mutants with altered stability |
EP2298903A3 (en) * | 2000-08-01 | 2011-10-05 | Novozymes A/S | Alpha-amylase mutants with altered properties |
JP2004508815A (en) * | 2000-08-01 | 2004-03-25 | ノボザイムス アクティーゼルスカブ | Alpha-amylase mutants with altered properties |
EP2308980A2 (en) | 2000-08-01 | 2011-04-13 | Novozymes A/S | Alpha-amylase mutants with altered properties |
WO2002038787A3 (en) * | 2000-11-10 | 2002-09-26 | Novozymes As | Secondary liquefaction of starch in ethanol production |
US7244597B2 (en) | 2000-11-10 | 2007-07-17 | Novozymes A/S | Secondary liquefaction in ethanol production |
WO2002038787A2 (en) * | 2000-11-10 | 2002-05-16 | Novozymes A/S | Secondary liquefaction of starch in ethanol production |
US9670471B2 (en) | 2001-05-15 | 2017-06-06 | Novozymes A/S | Alpha-amylase variant with altered properties |
US8617837B2 (en) | 2001-05-15 | 2013-12-31 | Novozymes A/S | Method of hydrolyzing soluble starch with an alpha-amylase variant |
EP3000881A2 (en) | 2001-05-15 | 2016-03-30 | Novozymes A/S | Alpha-amylase variant with altered properties |
US7498158B2 (en) | 2001-05-15 | 2009-03-03 | Novozymes A/S | Alpha-amylase variant with altered properties |
EP2264160A2 (en) | 2001-05-15 | 2010-12-22 | Novozymes A/S | Alpha-amylase variant with altered properties |
EP2159279A2 (en) | 2001-05-15 | 2010-03-03 | Novozymes A/S | Alpha-amylase variant with altered properties |
WO2002092797A3 (en) * | 2001-05-15 | 2004-03-25 | Novozymes As | Alpha-amylase variant with altered properties |
US9796968B2 (en) | 2001-05-15 | 2017-10-24 | Novozymes A/S | Alpha-amylase variant with altered properties |
US9080137B2 (en) | 2001-05-15 | 2015-07-14 | Novozymes A/S | Alpha-amylase variant with altered properties |
WO2002092797A2 (en) | 2001-05-15 | 2002-11-21 | Novozymes A/S | Alpha-amylase variant with altered properties |
EP2159279A3 (en) * | 2001-05-15 | 2010-05-12 | Novozymes A/S | Alpha-amylase variant with altered properties |
US10612012B2 (en) | 2001-05-15 | 2020-04-07 | Novozymes A/S | Alpha-amylase variant with altered properties |
US8486681B2 (en) | 2001-05-15 | 2013-07-16 | Novozymes A/S | Alpha-amylase variant with altered properties |
US8252573B2 (en) | 2001-05-15 | 2012-08-28 | Novozymes A/S | Alpha-amylase variant with altered properties |
EP2295545A1 (en) | 2002-09-26 | 2011-03-16 | Novozymes North America, Inc. | Fermentation methods and compositions |
EP2500423B1 (en) | 2003-02-26 | 2015-06-17 | Danisco US Inc. | Amylases producing an altered immunogenic response and methods of making and using the same |
DE10362172B4 (en) * | 2003-03-05 | 2009-04-02 | Henkel Ag & Co. Kgaa | Detergents and cleaners with alpha-amylase variants with improved alkalinity |
WO2004080923A2 (en) | 2003-03-10 | 2004-09-23 | Novozymes A/S | Alcohol product processes |
WO2004106533A1 (en) | 2003-05-30 | 2004-12-09 | Novozymes A/S | Alcohol product processes |
WO2005005646A2 (en) | 2003-06-10 | 2005-01-20 | Novozymes North America, Inc. | Fermentation processes and compositions |
WO2005003311A2 (en) | 2003-06-25 | 2005-01-13 | Novozymes A/S | Enzymes for starch processing |
WO2004113551A1 (en) | 2003-06-25 | 2004-12-29 | Novozymes A/S | Process for the hydrolysis of starch |
EP2336308A2 (en) | 2003-06-25 | 2011-06-22 | Novozymes A/S | Enzymes for starch processing |
EP2213732A1 (en) | 2003-10-28 | 2010-08-04 | Novozymes North America, Inc. | Hybrid glucoamylases |
WO2005111203A2 (en) * | 2004-04-08 | 2005-11-24 | Genencor International, Inc. | MUTANT α ΑMYLASES |
WO2005111203A3 (en) * | 2004-04-08 | 2006-03-30 | Genencor Int | MUTANT α ΑMYLASES |
EP3620523A2 (en) | 2004-07-05 | 2020-03-11 | Novozymes A/S | Alpha-amylase variants with altered properties |
EP4269684A2 (en) | 2004-07-05 | 2023-11-01 | Novozymes A/S | Alpha-amylase variants with altered properties |
WO2006002643A2 (en) | 2004-07-05 | 2006-01-12 | Novozymes A/S | Alpha-amylase variants with altered properties |
US8309337B2 (en) | 2004-08-02 | 2012-11-13 | Novozymes A/S | Hybrid polypeptide of a maltogenic alpha-amylase and a cyclodextrin glucanotransferase |
US8017371B2 (en) | 2004-08-02 | 2011-09-13 | Novozymes A/S | Hybrid polypeptide of a maltogenic alpha-amylase and a cyclodextrin glucanotransferase |
EP2258837A1 (en) | 2004-09-10 | 2010-12-08 | Novozymes North America, Inc. | Methods for preventing, removing, reducing, or disrupting biofilm |
EP2258836A1 (en) | 2004-09-10 | 2010-12-08 | Novozymes North America, Inc. | Methods for preventing, removing, reducing, or disrupting biofilm |
US8080401B2 (en) | 2004-10-01 | 2011-12-20 | Henkel Ag & Co. Kgaa | Alpha-amylase variants having an elevated solvent stability, method for the production thereof and detergents and cleansers containing these alpha-amylase variants |
DE102004047777B4 (en) | 2004-10-01 | 2018-05-09 | Basf Se | Alpha-amylase variants with increased solvent stability, process for their preparation and their use |
WO2006069289A2 (en) | 2004-12-22 | 2006-06-29 | Novozymes North America, Inc | Polypeptides having glucoamylase activity and polynucleotides encoding same |
WO2006069290A2 (en) | 2004-12-22 | 2006-06-29 | Novozymes A/S | Enzymes for starch processing |
EP2365068A2 (en) | 2004-12-22 | 2011-09-14 | Novozymes A/S | Enzymes for starch processing |
US8017351B2 (en) | 2005-06-24 | 2011-09-13 | Novozymes A/S | Amylases for pharmaceutical use |
WO2007028088A2 (en) | 2005-09-02 | 2007-03-08 | Novozymes North America, Inc. | Methods for enhancing the dewaterability of sludge with alpha-amylase treatment |
US8216817B2 (en) * | 2005-09-20 | 2012-07-10 | Novozymes North America, Inc. | Process of producing a fermentation product |
WO2007035730A2 (en) | 2005-09-20 | 2007-03-29 | Novozymes North America, Inc. | Process of producing a fermentation product |
WO2007076388A2 (en) | 2005-12-22 | 2007-07-05 | Novozymes North America, Inc. | Processes for producing a fermentation product |
WO2007109750A2 (en) | 2006-03-22 | 2007-09-27 | Novozymes North America, Inc. | Fermentation processes |
WO2007113292A2 (en) | 2006-04-04 | 2007-10-11 | Novozymes A/S | Mashing process |
EP2400026A1 (en) | 2006-06-06 | 2011-12-28 | Genecor International, Inc. | Process for conversion of granular starch to ethanol |
US7968318B2 (en) | 2006-06-06 | 2011-06-28 | Genencor International, Inc. | Process for conversion of granular starch to ethanol |
US8399224B2 (en) | 2007-03-14 | 2013-03-19 | Danisco Us Inc. | Production of ethanol from barley and DDGS containing reduced beta-glucan and phytic acid |
US8809031B2 (en) | 2007-03-23 | 2014-08-19 | Danisco Us Inc. | Enhanced amylase production by N-terminal addition to mature amylase protein |
EP3219804A1 (en) | 2007-04-24 | 2017-09-20 | Novozymes North America, Inc. | Detoxifying pre-treated lignocellulose-containing materials |
WO2008134259A1 (en) | 2007-04-24 | 2008-11-06 | Novozymes North America, Inc. | Detoxifying pre-treated lignocellulose-containing materials |
WO2008153815A2 (en) * | 2007-05-30 | 2008-12-18 | Danisco Us, Inc., Genencor Division | Variants of an alpha-amylase with improved production levels in fermentation processes |
US8546121B2 (en) | 2007-05-30 | 2013-10-01 | Danisco Us Inc. | Variants of an alpha-amylase with improved production levels in fermentation processes |
WO2008153815A3 (en) * | 2007-05-30 | 2009-02-05 | Danisco Us Inc Genencor Div | Variants of an alpha-amylase with improved production levels in fermentation processes |
US9334466B2 (en) | 2007-05-30 | 2016-05-10 | Danisco Us Inc. | Alpha-amylase variants |
WO2009030713A1 (en) | 2007-09-03 | 2009-03-12 | Novozymes A/S | Detoxifying and recycling of washing solution used in pretreatment of lignocellulose-containing materials |
US8206966B2 (en) | 2007-11-05 | 2012-06-26 | Danisco Us Inc. | Alpha-amylase variants with altered properties |
EP2215202B1 (en) | 2007-11-05 | 2017-09-06 | Danisco US Inc. | VARIANTS OF BACILLUS sp. TS-23 ALPHA-AMYLASE WITH ALTERED PROPERTIES |
US8153412B2 (en) | 2007-11-05 | 2012-04-10 | Danisco Us Inc. | Variants of Bacillus sp. TS-23 alpha-amylase with altered properties |
US8962283B2 (en) | 2008-02-04 | 2015-02-24 | Danisco Us Inc. | TS-23 alpha-amylase variants with altered properties |
US8507244B2 (en) | 2008-02-04 | 2013-08-13 | Danisco Us Inc. | Variants of bacillus sp. TS-23 alpha-amylase with altered properties |
US8460916B2 (en) | 2008-02-04 | 2013-06-11 | Danisco Us Inc. | TS-23 alpha-amylase variants with altered properties |
US8236545B2 (en) | 2008-02-04 | 2012-08-07 | Danisco Us Inc., Genencor Division | TS23 alpha-amylase variants with altered properties |
EP2447361A2 (en) | 2008-06-06 | 2012-05-02 | Danisco US Inc. | Geobacillus stearothermophilus alpha-amylase (AMYS) variants with improved properties |
EP2623591A2 (en) | 2008-06-06 | 2013-08-07 | Danisco US Inc. | Geobacillus stearothermophilus alpha-amylase (AMYS) variants with improved properties |
US8084240B2 (en) | 2008-06-06 | 2011-12-27 | Danisco Us Inc. | Geobacillus stearothermophilus α-amylase (AmyS) variants with improved properties |
WO2010008841A2 (en) | 2008-06-23 | 2010-01-21 | Novozymes A/S | Processes for producing fermentation products |
US8507243B2 (en) | 2008-09-25 | 2013-08-13 | Danisco Us Inc. | Alpha-amylase blends and methods for using said blends |
WO2010039812A2 (en) | 2008-09-30 | 2010-04-08 | Novozymes North America, Inc. | Improvement of enzymatic hydrolysis of pre-treated lignocellulose-containing material with distillers dried grains |
WO2010043538A2 (en) | 2008-10-15 | 2010-04-22 | Novozymes A/S | Brewing process |
WO2010059413A2 (en) | 2008-11-20 | 2010-05-27 | Novozymes, Inc. | Polypeptides having amylolytic enhancing activity and polynucleotides encoding same |
EP2857515A2 (en) | 2008-11-20 | 2015-04-08 | Novozymes Inc. | Polypeptides having amylolytic enhancing activity and polynucleotides encoding same |
WO2010078391A2 (en) | 2008-12-30 | 2010-07-08 | Novozymes North America, Inc. | Improvement of enzymatic hydrolysis of pretreated lignocellulose-containing material with dissolved air flotation sludge |
WO2010078392A2 (en) | 2008-12-31 | 2010-07-08 | Novozymes North America, Inc. | Processes of producing fermentation products |
CN102341495A (en) * | 2009-03-10 | 2012-02-01 | 丹尼斯科美国公司 | ALPHA-AMYLASES ASSOCIATED with BACILLUS MEGATERIUM DSM90, and method for using same |
WO2010104675A1 (en) * | 2009-03-10 | 2010-09-16 | Danisco Us Inc. | Bacillus megaterium strain dsm90-related alpha-amylases, and methods of use, thereof |
WO2010115021A2 (en) | 2009-04-01 | 2010-10-07 | Danisco Us Inc. | Compositions and methods comprising alpha-amylase variants with altered properties |
US8852912B2 (en) | 2009-04-01 | 2014-10-07 | Danisco Us Inc. | Compositions and methods comprising alpha-amylase variants with altered properties |
EP2902487A2 (en) | 2009-04-01 | 2015-08-05 | Danisco US Inc. | Compositions and methods comprising alpha-amylase variants with altered properties |
WO2011003940A1 (en) | 2009-07-07 | 2011-01-13 | Novozymes A/S | Process for treating a substrate with an enzyme |
WO2011008785A2 (en) | 2009-07-17 | 2011-01-20 | Novozymes A/S | A method of analyzing cellulose decay in cellulosic material hydrolysis |
WO2011017093A1 (en) | 2009-08-07 | 2011-02-10 | Danisco Us Inc. | Alpha-amylase blend for starch processing and method of use thereof |
US8545907B2 (en) | 2009-08-07 | 2013-10-01 | Danisco Us Inc. | Alpha-amylase blend for starch processing and method of use thereof |
US8815560B2 (en) | 2009-08-07 | 2014-08-26 | Danisco Us Inc. | Alpha-amylase blend for starch processing and method of use thereof |
WO2011020910A1 (en) | 2009-08-21 | 2011-02-24 | Novozymes A/S | Polypeptides having isoamylase activity and polynucleotides encoding same |
WO2011066560A1 (en) | 2009-11-30 | 2011-06-03 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
WO2011066576A1 (en) | 2009-11-30 | 2011-06-03 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
WO2011068803A1 (en) | 2009-12-01 | 2011-06-09 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
WO2011087836A2 (en) | 2009-12-22 | 2011-07-21 | Novozymes A/S | Pullulanase variants and uses thereof |
WO2011080353A1 (en) | 2010-01-04 | 2011-07-07 | Novozymes A/S | Stabilization of alpha-amylases towards calcium depletion and acidic ph |
WO2011082425A2 (en) | 2010-01-04 | 2011-07-07 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2011080352A1 (en) | 2010-01-04 | 2011-07-07 | Novozymes A/S | Alpha-amylases |
WO2011080354A1 (en) | 2010-01-04 | 2011-07-07 | Novozymes A/S | Alpha-amylases |
WO2011082429A1 (en) | 2010-01-04 | 2011-07-07 | Novozymes A/S | Alpha-amylases |
EP3101127A1 (en) | 2010-01-04 | 2016-12-07 | Novozymes A/S | Alpha-amylase variants with improved stability |
WO2011092136A1 (en) | 2010-01-29 | 2011-08-04 | Novozymes A/S | Biogas production process with enzymatic pre-treatment |
WO2011100161A1 (en) | 2010-02-09 | 2011-08-18 | Novozymes North America, Inc. | Addition of alpha - glucosidase and cobalt for producing fermentation products from starch |
US11453868B2 (en) | 2010-02-10 | 2022-09-27 | Novozymes A/S | Variants and compositions comprising variants with high stability in presence of a chelating agent |
EP3404087A1 (en) * | 2010-02-10 | 2018-11-21 | Novozymes A/S | Alpha-amylase variants with high stability in presence of a chelating agent |
US10655116B2 (en) | 2010-02-10 | 2020-05-19 | Novozymes A/S | Variants and compositions comprising variants with high stability in presence of a chelating agent |
US11959107B2 (en) | 2010-02-10 | 2024-04-16 | Novozymes A/S | Variants and compositions comprising variants with high stability in presence of a chelating agent |
EP3892709A3 (en) * | 2010-02-10 | 2022-01-19 | Novozymes A/S | Variants and compositions comprising variants with high stability in presence of a chelating agent |
WO2011126897A2 (en) | 2010-03-30 | 2011-10-13 | Novozymes A/S | Methods for enhancing by-products from fermentation processes |
EP3070171A1 (en) | 2010-03-30 | 2016-09-21 | Novozymes A/S | Processes of producing a fermentation product |
WO2011123505A1 (en) | 2010-03-30 | 2011-10-06 | Novozymes North America, Inc. | Processes of producing a fermentation product |
US9617527B2 (en) | 2010-04-14 | 2017-04-11 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
US10196620B2 (en) | 2010-04-14 | 2019-02-05 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
WO2011127802A1 (en) | 2010-04-14 | 2011-10-20 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
WO2011127820A1 (en) | 2010-04-14 | 2011-10-20 | Novozymes A/S | Processes for producing fermentation products |
US11279920B2 (en) | 2010-04-14 | 2022-03-22 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
WO2012018775A1 (en) | 2010-08-02 | 2012-02-09 | Novozymes North America, Inc. | Process of producing a fermentation product |
WO2012064351A1 (en) | 2010-11-08 | 2012-05-18 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
WO2012064350A1 (en) | 2010-11-08 | 2012-05-18 | Novozymes A/S | Polypeptides Having Glucoamylase Activity and Polynucleotides Encoding Same |
WO2012068047A2 (en) | 2010-11-19 | 2012-05-24 | Novozymes North America, Inc. | Processes of producing a fermentation product |
US10947567B2 (en) | 2010-12-22 | 2021-03-16 | Novozymes A/S | Processes for producing ethanol |
US11840718B2 (en) | 2010-12-22 | 2023-12-12 | Novozymes A/S | Processes for producing ethanol |
US11499170B2 (en) | 2010-12-22 | 2022-11-15 | Novozymes A/S | Processes for producing ethanol |
US11566266B2 (en) | 2010-12-22 | 2023-01-31 | Novozymes A/S | Processes for producing ethanol |
CN103608460A (en) * | 2010-12-22 | 2014-02-26 | 诺维信北美公司 | Processes for producing fermentation products |
US9816112B2 (en) | 2010-12-22 | 2017-11-14 | Novozymes A/S | Processes for producing fermentation products |
WO2012088303A2 (en) | 2010-12-22 | 2012-06-28 | Novozymes North America, Inc. | Processes for producing fermentation products |
US10941422B2 (en) | 2010-12-22 | 2021-03-09 | Novozymes A/S | Processes for producing fuel ethanol |
CN103608460B (en) * | 2010-12-22 | 2017-08-29 | 诺维信北美公司 | For the technique for producing tunning |
WO2012088303A3 (en) * | 2010-12-22 | 2013-10-31 | Novozymes North America, Inc. | Process for producing fermentation products from starch containing materials |
WO2012093041A1 (en) | 2011-01-04 | 2012-07-12 | Novozymes A/S | Process for producing biogas from pectin and lignocellulose containing material |
WO2012109119A2 (en) | 2011-02-07 | 2012-08-16 | Novozymes North America, Inc. | Process of producing a fermentation product |
WO2012140075A2 (en) | 2011-04-15 | 2012-10-18 | Novozymes A/S | Method for production of brewers wort |
WO2012149275A1 (en) | 2011-04-29 | 2012-11-01 | Danisco Us Inc. | Use of cellulase and glucoamylase to improve ethanol yields from fermentation |
WO2013000945A1 (en) | 2011-06-28 | 2013-01-03 | Novozymes A/S | Biogas from enzyme-treated bagasse |
US10167458B2 (en) | 2011-06-30 | 2019-01-01 | Novozymes A/S | Alpha-amylase variants |
US9434932B2 (en) | 2011-06-30 | 2016-09-06 | Novozymes A/S | Alpha-amylase variants |
US10752889B2 (en) | 2011-06-30 | 2020-08-25 | Novozymes A/S | Alpha-amylase variants |
US11091748B2 (en) | 2011-06-30 | 2021-08-17 | Novozymes A/S | Alpha-amylase variants |
WO2013006756A2 (en) | 2011-07-06 | 2013-01-10 | Novozymes A/S | Alpha amylase variants and polynucleotides encoding same |
WO2013016115A1 (en) | 2011-07-22 | 2013-01-31 | Novozymes North America, Inc. | Processes for pretreating cellulosic material and improving hydrolysis thereof |
WO2013029496A1 (en) | 2011-08-26 | 2013-03-07 | Novozymes A/S | Polypeptides having glucoamylase activity and polynucleotides encoding same |
WO2013036526A1 (en) | 2011-09-06 | 2013-03-14 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
US20140273135A1 (en) * | 2011-10-11 | 2014-09-18 | Novozymes A/S | Processes for producing fermentation products |
US9677095B2 (en) * | 2011-10-11 | 2017-06-13 | Novozymes A/S | Processes for producing fermentation products |
WO2013053801A1 (en) | 2011-10-11 | 2013-04-18 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
WO2013055676A1 (en) | 2011-10-11 | 2013-04-18 | Novozymes North America, Inc. | Processes for producing fermentation products |
US10041054B2 (en) * | 2011-10-11 | 2018-08-07 | Novozymes A/S | Processes for producing fermentation products |
WO2013057143A2 (en) | 2011-10-17 | 2013-04-25 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
EP4253534A2 (en) | 2011-10-17 | 2023-10-04 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
EP3495479A1 (en) | 2011-10-17 | 2019-06-12 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2013057141A2 (en) | 2011-10-17 | 2013-04-25 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
US10457924B2 (en) | 2011-10-28 | 2019-10-29 | Danisco Us Inc | Variant maltohexaose-forming alpha-amylase variants |
US11898177B2 (en) | 2011-10-28 | 2024-02-13 | Danisco Us Inc. | Variant maltohexaose-forming alpha-amylase variants |
WO2013082486A1 (en) | 2011-12-02 | 2013-06-06 | Novozymes A/S | Processes for producing fermentation products |
WO2013083801A2 (en) | 2011-12-09 | 2013-06-13 | Novozymes A/S | Biogas from substrates comprising animal manure and enzymes |
WO2013096652A1 (en) | 2011-12-21 | 2013-06-27 | Novozymes, Inc. | Methods for determining the degradation of a biomass material |
WO2013110766A1 (en) | 2012-01-26 | 2013-08-01 | Novozymes A/S | Use of polypeptides having protease activity in animal feed and detergents |
WO2013148993A1 (en) | 2012-03-30 | 2013-10-03 | Novozymes North America, Inc. | Processes of producing fermentation products |
EP4209595A1 (en) | 2012-03-30 | 2023-07-12 | Novozymes North America, Inc. | A method of dewatering whole stillage |
WO2013167581A1 (en) | 2012-05-07 | 2013-11-14 | Novozymes A/S | Polypeptides having xanthan degrading activity and polynucleotides encoding same |
WO2013167573A1 (en) | 2012-05-11 | 2013-11-14 | Novozymes A/S | A brewing method |
WO2013171241A1 (en) | 2012-05-16 | 2013-11-21 | Novozymes A/S | Compositions comprising lipase and methods of use thereof |
EP2859097B1 (en) | 2012-06-08 | 2021-11-03 | Danisco US Inc. | Alpha-amylase variants derived from the alpha amylase of cytophaga sp. amylasei(cspamy2). |
WO2013189972A2 (en) | 2012-06-20 | 2013-12-27 | Novozymes A/S | Use of polypeptides having protease activity in animal feed and detergents |
WO2014006140A1 (en) | 2012-07-06 | 2014-01-09 | Novozymes A/S | Liquid enzyme composition and method for enzyme recovery |
WO2014027062A1 (en) | 2012-08-17 | 2014-02-20 | Novozymes A/S | Thermostable asparaginase variants and polynucleotides encoding same |
WO2014029821A1 (en) | 2012-08-22 | 2014-02-27 | Novozymes A/S | Metalloproteases from alicyclobacillus sp. |
WO2014029819A1 (en) | 2012-08-22 | 2014-02-27 | Novozymes A/S | Metalloprotease from exiguobacterium |
WO2014060474A1 (en) | 2012-10-17 | 2014-04-24 | Novozymes A/S | Method for production of brewers wort |
WO2014087011A1 (en) | 2012-12-07 | 2014-06-12 | Novozymes A/S | Preventing adhesion of bacteria |
EP3556836A1 (en) | 2012-12-07 | 2019-10-23 | Novozymes A/S | Preventing adhesion of bacteria |
EP3321353A1 (en) | 2012-12-11 | 2018-05-16 | Danisco US Inc. | Yeast host cells epxressing a glucoamylase from aspergillus fumigatus and methods of use thereof |
WO2014092960A1 (en) | 2012-12-11 | 2014-06-19 | Danisco Us Inc. | Trichoderma reesei host cells expressing a glucoamylase from aspergillus fumigatus and methods of use thereof |
WO2014096259A1 (en) | 2012-12-21 | 2014-06-26 | Novozymes A/S | Polypeptides having protease activiy and polynucleotides encoding same |
EP3321360A2 (en) | 2013-01-03 | 2018-05-16 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
EP3569611A1 (en) | 2013-04-23 | 2019-11-20 | Novozymes A/S | Liquid automatic dish washing detergent compositions with stabilised subtilisin |
WO2014177541A2 (en) | 2013-04-30 | 2014-11-06 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
EP3372680A1 (en) | 2013-04-30 | 2018-09-12 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
EP3461881A1 (en) | 2013-05-03 | 2019-04-03 | Novozymes A/S | Microencapsulation of detergent enzymes |
WO2014184164A1 (en) | 2013-05-14 | 2014-11-20 | Novozymes A/S | Detergent compositions |
WO2014183921A1 (en) | 2013-05-17 | 2014-11-20 | Novozymes A/S | Polypeptides having alpha amylase activity |
EP3260538A1 (en) | 2013-05-29 | 2017-12-27 | Danisco US Inc. | Novel metalloproteases |
WO2014194034A2 (en) | 2013-05-29 | 2014-12-04 | Danisco Us Inc. | Novel metalloproteases |
EP3636662A1 (en) | 2013-05-29 | 2020-04-15 | Danisco US Inc. | Novel metalloproteases |
EP3882346A1 (en) | 2013-05-29 | 2021-09-22 | Danisco US Inc. | Novel metalloproteases |
WO2014194032A1 (en) | 2013-05-29 | 2014-12-04 | Danisco Us Inc. | Novel metalloproteases |
WO2014194117A2 (en) | 2013-05-29 | 2014-12-04 | Danisco Us Inc. | Novel metalloproteases |
EP4159854A1 (en) | 2013-05-29 | 2023-04-05 | Danisco US Inc | Novel metalloproteases |
WO2014194054A1 (en) | 2013-05-29 | 2014-12-04 | Danisco Us Inc. | Novel metalloproteases |
EP3786269A1 (en) | 2013-06-06 | 2021-03-03 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2014209800A1 (en) | 2013-06-24 | 2014-12-31 | Novozymes A/S | Processes for recovering oil from fermentation product processes and processes for producing fermentation products |
US10781398B2 (en) | 2013-06-24 | 2020-09-22 | Novozymes A/S | Process of recovering oil |
US11965143B2 (en) | 2013-06-24 | 2024-04-23 | Novozymes A/S | Process of recovering oil |
US10844318B2 (en) | 2013-06-24 | 2020-11-24 | Novozymes A/S | Processes for recovering oil from ethanol production processes |
US10731104B2 (en) | 2013-06-24 | 2020-08-04 | Novozymes A/S | Process of recovering oil |
US11505765B2 (en) | 2013-06-24 | 2022-11-22 | Novozymes A/S | Process of recovering oil |
US11939552B2 (en) | 2013-06-24 | 2024-03-26 | Novozymes A/S | Process of recovering oil |
WO2014206960A1 (en) | 2013-06-26 | 2014-12-31 | Novozymes A/S | Process for manufacturing a feed composition |
WO2014207224A1 (en) | 2013-06-27 | 2014-12-31 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
WO2014207227A1 (en) | 2013-06-27 | 2014-12-31 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
WO2015001017A2 (en) | 2013-07-04 | 2015-01-08 | Novozymes A/S | Polypeptides having anti-redeposition effect and polynucleotides encoding same |
WO2015007639A1 (en) | 2013-07-17 | 2015-01-22 | Novozymes A/S | Pullulanase chimeras and polynucleotides encoding same |
EP3309249A1 (en) | 2013-07-29 | 2018-04-18 | Novozymes A/S | Protease variants and polynucleotides encoding same |
EP3611260A1 (en) | 2013-07-29 | 2020-02-19 | Novozymes A/S | Protease variants and polynucleotides encoding same |
EP3613853A1 (en) | 2013-07-29 | 2020-02-26 | Novozymes A/S | Protease variants and polynucleotides encoding same |
WO2015035914A1 (en) | 2013-09-11 | 2015-03-19 | Novozymes A/S | Processes for producing fermentation products |
EP3712274A1 (en) | 2013-09-11 | 2020-09-23 | Novozymes A/S | Processes for producing fermentation products |
WO2015049370A1 (en) | 2013-10-03 | 2015-04-09 | Novozymes A/S | Detergent composition and use of detergent composition |
WO2015057517A1 (en) | 2013-10-17 | 2015-04-23 | Danisco Us Inc. | Use of hemicellulases to improve ethanol production |
EP3514230A1 (en) | 2013-12-13 | 2019-07-24 | Danisco US Inc. | Serine proteases of bacillus species |
WO2015089447A1 (en) | 2013-12-13 | 2015-06-18 | Danisco Us Inc. | Serine proteases of the bacillus gibsonii-clade |
WO2015089441A1 (en) | 2013-12-13 | 2015-06-18 | Danisco Us Inc. | Serine proteases of bacillus species |
EP3553173A1 (en) | 2013-12-13 | 2019-10-16 | Danisco US Inc. | Serine proteases of the bacillus gibsonii-clade |
EP3910057A1 (en) | 2013-12-13 | 2021-11-17 | Danisco US Inc. | Serine proteases of the bacillus gibsonii-clade |
EP4163305A1 (en) | 2013-12-16 | 2023-04-12 | Nutrition & Biosciences USA 4, Inc. | Use of poly alpha-1,3-glucan ethers as viscosity modifiers |
EP3789407A1 (en) | 2013-12-18 | 2021-03-10 | Nutrition & Biosciences USA 4, Inc. | Cationic poly alpha-1,3-glucan ethers |
WO2015095358A1 (en) | 2013-12-18 | 2015-06-25 | E. I. Du Pont De Nemours And Company | Cationic poly alpha-1,3-glucan ethers |
EP3453757A1 (en) | 2013-12-20 | 2019-03-13 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
WO2015091959A3 (en) * | 2013-12-20 | 2015-08-13 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
EP3415624A1 (en) | 2014-01-22 | 2018-12-19 | Novozymes A/S | Pullulanase variants and polynucleotides encoding same |
WO2015123323A1 (en) | 2014-02-14 | 2015-08-20 | E. I. Du Pont De Nemours And Company | Poly-alpha-1,3-1,6-glucans for viscosity modification |
WO2015134737A1 (en) | 2014-03-05 | 2015-09-11 | Novozymes A/S | Compositions and methods for improving properties of cellulosic textile materials with xyloglucan endotransglycosylase |
WO2015134729A1 (en) | 2014-03-05 | 2015-09-11 | Novozymes A/S | Compositions and methods for improving properties of non-cellulosic textile materials with xyloglucan endotransglycosylase |
WO2015138283A1 (en) | 2014-03-11 | 2015-09-17 | E. I. Du Pont De Nemours And Company | Oxidized poly alpha-1,3-glucan as detergent builder |
WO2015143144A1 (en) | 2014-03-19 | 2015-09-24 | Novozymes A/S | Method for enhancing activity of an x143 polypeptide |
EP4155398A1 (en) | 2014-03-21 | 2023-03-29 | Danisco US Inc. | Serine proteases of bacillus species |
EP3587569A1 (en) | 2014-03-21 | 2020-01-01 | Danisco US Inc. | Serine proteases of bacillus species |
WO2015150457A1 (en) | 2014-04-01 | 2015-10-08 | Novozymes A/S | Polypeptides having alpha amylase activity |
EP3550015A1 (en) | 2014-04-10 | 2019-10-09 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
EP3722406A1 (en) | 2014-04-11 | 2020-10-14 | Novozymes A/S | Detergent composition |
EP3878957A1 (en) | 2014-05-27 | 2021-09-15 | Novozymes A/S | Methods for producing lipases |
EP3760713A2 (en) | 2014-05-27 | 2021-01-06 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
WO2015181119A2 (en) | 2014-05-27 | 2015-12-03 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
WO2015189371A1 (en) | 2014-06-12 | 2015-12-17 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2015195960A1 (en) | 2014-06-19 | 2015-12-23 | E. I. Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
EP3919599A1 (en) | 2014-06-19 | 2021-12-08 | Nutrition & Biosciences USA 4, Inc. | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
WO2015195777A1 (en) | 2014-06-19 | 2015-12-23 | E. I. Du Pont De Nemours And Company | Compositions containing one or more poly alpha-1,3-glucan ether compounds |
WO2015200659A1 (en) | 2014-06-25 | 2015-12-30 | Novozymes A/S | Xylanase variants and polynucleotides encoding same |
WO2016001319A1 (en) | 2014-07-03 | 2016-01-07 | Novozymes A/S | Improved stabilization of non-protease enzyme |
EP3878960A1 (en) | 2014-07-04 | 2021-09-15 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
EP3739029A1 (en) | 2014-07-04 | 2020-11-18 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
WO2016061438A1 (en) | 2014-10-17 | 2016-04-21 | Danisco Us Inc. | Serine proteases of bacillus species |
WO2016062875A2 (en) | 2014-10-23 | 2016-04-28 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
WO2016069544A1 (en) | 2014-10-27 | 2016-05-06 | Danisco Us Inc. | Serine proteases |
WO2016069552A1 (en) | 2014-10-27 | 2016-05-06 | Danisco Us Inc. | Serine proteases |
EP3550017A1 (en) | 2014-10-27 | 2019-10-09 | Danisco US Inc. | Serine proteases |
WO2016069557A1 (en) | 2014-10-27 | 2016-05-06 | Danisco Us Inc. | Serine proteases of bacillus species |
WO2016069548A2 (en) | 2014-10-27 | 2016-05-06 | Danisco Us Inc. | Serine proteases |
WO2016069569A2 (en) | 2014-10-27 | 2016-05-06 | Danisco Us Inc. | Serine proteases |
WO2016079110A2 (en) | 2014-11-19 | 2016-05-26 | Novozymes A/S | Use of enzyme for cleaning |
WO2016079305A1 (en) | 2014-11-20 | 2016-05-26 | Novozymes A/S | Alicyclobacillus variants and polynucleotides encoding same |
WO2016087327A1 (en) | 2014-12-01 | 2016-06-09 | Novozymes A/S | Polypeptides having pullulanase activity comprising the x25, x45 and cbm41 domains |
EP3690037A1 (en) | 2014-12-04 | 2020-08-05 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
EP4067485A2 (en) | 2014-12-05 | 2022-10-05 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
WO2016087401A1 (en) | 2014-12-05 | 2016-06-09 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
US10760036B2 (en) | 2014-12-15 | 2020-09-01 | Henkel Ag & Co. Kgaa | Detergent composition comprising subtilase variants |
EP3608403A2 (en) | 2014-12-15 | 2020-02-12 | Henkel AG & Co. KGaA | Detergent composition comprising subtilase variants |
WO2016096996A1 (en) | 2014-12-16 | 2016-06-23 | Novozymes A/S | Polypeptides having n-acetyl glucosamine oxidase activity |
WO2016100324A1 (en) | 2014-12-17 | 2016-06-23 | The Procter & Gamble Company | Method of automatic dishwashing |
WO2016099861A1 (en) | 2014-12-17 | 2016-06-23 | The Procter & Gamble Company | Method of automatic dishwashing |
EP3034588A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Detergent composition |
EP3034589A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Detergent composition |
EP3034591A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Method of automatic dishwashing |
EP3034590A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Method of automatic dishwashing |
EP3034596A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Detergent composition |
EP3034592A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Method of automatic dishwashing |
EP3034597A1 (en) | 2014-12-17 | 2016-06-22 | The Procter and Gamble Company | Detergent composition |
US10266796B2 (en) | 2014-12-17 | 2019-04-23 | The Procter & Gamble Company | Detergent composition |
WO2016099860A1 (en) | 2014-12-17 | 2016-06-23 | The Procter & Gamble Company | Method of automatic dishwashing |
US10662398B2 (en) | 2014-12-17 | 2020-05-26 | The Procter & Gamble Company | Detergent composition |
WO2016100320A1 (en) | 2014-12-17 | 2016-06-23 | The Procter & Gamble Company | Detergent composition |
US10081782B2 (en) | 2014-12-17 | 2018-09-25 | The Procter & Gamble Company | Detergent composition |
WO2016100323A1 (en) | 2014-12-17 | 2016-06-23 | The Procter & Gamble Company | Detergent composition |
WO2016099858A1 (en) | 2014-12-17 | 2016-06-23 | The Procter & Gamble Company | Detergent composition |
WO2016099859A1 (en) | 2014-12-17 | 2016-06-23 | The Procter & Gamble Company | Detergent composition |
EP3741849A2 (en) | 2014-12-19 | 2020-11-25 | Novozymes A/S | Protease variants and polynucleotides encoding same |
EP3741848A2 (en) | 2014-12-19 | 2020-11-25 | Novozymes A/S | Protease variants and polynucleotides encoding same |
WO2016106011A1 (en) | 2014-12-23 | 2016-06-30 | E. I. Du Pont De Nemours And Company | Enzymatically produced cellulose |
WO2016162556A1 (en) | 2015-04-10 | 2016-10-13 | Novozymes A/S | Laundry method, use of dnase and detergent composition |
WO2016162558A1 (en) | 2015-04-10 | 2016-10-13 | Novozymes A/S | Detergent composition |
EP4219704A2 (en) | 2015-05-13 | 2023-08-02 | Danisco US Inc | Aprl-clade protease variants and uses thereof |
EP3872174A1 (en) | 2015-05-13 | 2021-09-01 | Danisco US Inc. | Aprl-clade protease variants and uses thereof |
WO2016184944A1 (en) | 2015-05-19 | 2016-11-24 | Novozymes A/S | Odor reduction |
WO2016196202A1 (en) | 2015-05-29 | 2016-12-08 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
US10377974B2 (en) | 2015-06-04 | 2019-08-13 | The Procter & Gamble Company | Hand dishwashing liquid detergent composition |
US10377973B2 (en) | 2015-06-04 | 2019-08-13 | The Procter & Gamble Company | Hand dishwashing liquid detergent composition |
EP3101108A1 (en) | 2015-06-04 | 2016-12-07 | The Procter and Gamble Company | Hand dishwashing liquid detergent composition |
EP3101109A1 (en) | 2015-06-04 | 2016-12-07 | The Procter and Gamble Company | Hand dishwashing liquid detergent composition |
WO2016196872A1 (en) | 2015-06-04 | 2016-12-08 | The Procter & Gamble Company | Hand dishwashing liquid detergent composition |
WO2016196874A1 (en) | 2015-06-04 | 2016-12-08 | The Procter & Gamble Company | Hand dishwashing liquid detergent composition |
EP3287513A1 (en) | 2015-06-04 | 2018-02-28 | The Procter & Gamble Company | Hand dishwashing liquid detergent composition |
EP3284811A1 (en) | 2015-06-04 | 2018-02-21 | The Procter & Gamble Company | Hand dishwashing liquid detergent composition |
US10711225B2 (en) | 2015-06-05 | 2020-07-14 | The Procter & Gamble Company | Compacted liquid laundry detergent composition |
US10683474B2 (en) | 2015-06-05 | 2020-06-16 | The Procter & Gamble Company | Compacted liquid laundry detergent composition |
US10655093B2 (en) | 2015-06-05 | 2020-05-19 | The Procter & Gamble Company | Compacted liquid laundry detergent composition |
WO2016201044A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc | Osmotic burst encapsulates |
WO2016201069A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc | Low-density enzyme-containing particles |
WO2016201040A1 (en) | 2015-06-09 | 2016-12-15 | Danisco Us Inc. | Water-triggered enzyme suspension |
WO2016202739A1 (en) | 2015-06-16 | 2016-12-22 | Novozymes A/S | Polypeptides with lipase activity and polynucleotides encoding same |
EP4234693A2 (en) | 2015-06-17 | 2023-08-30 | Danisco US Inc | Bacillus gibsonii-clade serine proteases |
WO2016202785A1 (en) | 2015-06-17 | 2016-12-22 | Novozymes A/S | Container |
WO2016205755A1 (en) | 2015-06-17 | 2016-12-22 | Danisco Us Inc. | Bacillus gibsonii-clade serine proteases |
WO2016205127A1 (en) | 2015-06-18 | 2016-12-22 | Novozymes A/S | Polypeptides having trehalase activity and the use thereof in process of producing fermentation products |
EP4071244A1 (en) | 2015-06-18 | 2022-10-12 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
EP3872175A1 (en) | 2015-06-18 | 2021-09-01 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
EP3106508A1 (en) | 2015-06-18 | 2016-12-21 | Henkel AG & Co. KGaA | Detergent composition comprising subtilase variants |
WO2016135351A1 (en) | 2015-06-30 | 2016-09-01 | Novozymes A/S | Laundry detergent composition, method for washing and use of composition |
EP3929285A2 (en) | 2015-07-01 | 2021-12-29 | Novozymes A/S | Methods of reducing odor |
EP3950939A2 (en) | 2015-07-06 | 2022-02-09 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
WO2017015329A1 (en) | 2015-07-23 | 2017-01-26 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2017046232A1 (en) | 2015-09-17 | 2017-03-23 | Henkel Ag & Co. Kgaa | Detergent compositions comprising polypeptides having xanthan degrading activity |
WO2017046260A1 (en) | 2015-09-17 | 2017-03-23 | Novozymes A/S | Polypeptides having xanthan degrading activity and polynucleotides encoding same |
EP3708660A2 (en) | 2015-10-07 | 2020-09-16 | Novozymes A/S | Polypeptides |
WO2017060505A1 (en) | 2015-10-07 | 2017-04-13 | Novozymes A/S | Polypeptides |
EP4324919A2 (en) | 2015-10-14 | 2024-02-21 | Novozymes A/S | Polypeptide variants |
WO2017066510A1 (en) | 2015-10-14 | 2017-04-20 | Novozymes A/S | Cleaning of water filtration membranes |
WO2017064269A1 (en) | 2015-10-14 | 2017-04-20 | Novozymes A/S | Polypeptide variants |
WO2017066255A1 (en) | 2015-10-14 | 2017-04-20 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
WO2017064253A1 (en) | 2015-10-14 | 2017-04-20 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
EP4141113A1 (en) | 2015-11-05 | 2023-03-01 | Danisco US Inc | Paenibacillus sp. mannanases |
WO2017079756A1 (en) | 2015-11-05 | 2017-05-11 | Danisco Us Inc | Paenibacillus and bacillus spp. mannanases |
WO2017079751A1 (en) | 2015-11-05 | 2017-05-11 | Danisco Us Inc | Paenibacillus sp. mannanases |
WO2017083229A1 (en) | 2015-11-13 | 2017-05-18 | E. I. Du Pont De Nemours And Company | Glucan fiber compositions for use in laundry care and fabric care |
WO2017083228A1 (en) | 2015-11-13 | 2017-05-18 | E. I. Du Pont De Nemours And Company | Glucan fiber compositions for use in laundry care and fabric care |
WO2017083226A1 (en) | 2015-11-13 | 2017-05-18 | E. I. Du Pont De Nemours And Company | Glucan fiber compositions for use in laundry care and fabric care |
US10844324B2 (en) | 2015-11-13 | 2020-11-24 | Dupont Industrial Biosciences Usa, Llc | Glucan fiber compositions for use in laundry care and fabric care |
US10876074B2 (en) | 2015-11-13 | 2020-12-29 | Dupont Industrial Biosciences Usa, Llc | Glucan fiber compositions for use in laundry care and fabric care |
US10822574B2 (en) | 2015-11-13 | 2020-11-03 | Dupont Industrial Biosciences Usa, Llc | Glucan fiber compositions for use in laundry care and fabric care |
WO2017089366A1 (en) | 2015-11-24 | 2017-06-01 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
WO2017093318A1 (en) | 2015-12-01 | 2017-06-08 | Novozymes A/S | Methods for producing lipases |
US11441140B2 (en) | 2015-12-07 | 2022-09-13 | Henkel Ag & Co. Kgaa | Dishwashing compositions comprising polypeptides having beta-glucanase activity and uses thereof |
US11920170B2 (en) | 2015-12-09 | 2024-03-05 | Danisco Us Inc. | Alpha-amylase combinatorial variants |
WO2017106676A1 (en) | 2015-12-18 | 2017-06-22 | Danisco Us Inc | Polypeptides with endoglucanase activity and uses thereof |
WO2017112539A1 (en) | 2015-12-22 | 2017-06-29 | Novozymes A/S | Process of extracting oil from thin stillage |
WO2017112542A1 (en) | 2015-12-22 | 2017-06-29 | Novozymes A/S | Processes for improving fermentation product yield using phospholipase c |
WO2017112540A1 (en) | 2015-12-22 | 2017-06-29 | Novozymes A/S | Processes for producing fermentation products |
WO2017117089A1 (en) | 2015-12-28 | 2017-07-06 | Novozymes Bioag A/S | Heat priming of bacterial spores |
EP4219702A2 (en) | 2015-12-30 | 2023-08-02 | Novozymes A/S | Enzyme variants and polynucleotides encoding the same |
WO2017129754A1 (en) | 2016-01-29 | 2017-08-03 | Novozymes A/S | Beta-glucanase variants and polynucleotides encoding same |
EP3715442A1 (en) | 2016-03-23 | 2020-09-30 | Novozymes A/S | Use of polypeptide having dnase activity for treating fabrics |
WO2017174769A2 (en) | 2016-04-08 | 2017-10-12 | Novozymes A/S | Detergent compositions and uses of the same |
EP3693449A1 (en) | 2016-04-29 | 2020-08-12 | Novozymes A/S | Detergent compositions and uses thereof |
WO2017186943A1 (en) | 2016-04-29 | 2017-11-02 | Novozymes A/S | Detergent compositions and uses thereof |
WO2017191160A1 (en) | 2016-05-03 | 2017-11-09 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding the same |
WO2017192692A1 (en) | 2016-05-03 | 2017-11-09 | Danisco Us Inc | Protease variants and uses thereof |
EP3845642A1 (en) | 2016-05-05 | 2021-07-07 | Danisco US Inc. | Protease variants and uses thereof |
WO2017192300A1 (en) | 2016-05-05 | 2017-11-09 | Danisco Us Inc | Protease variants and uses thereof |
WO2017194487A1 (en) | 2016-05-09 | 2017-11-16 | Novozymes A/S | Variant polypeptides with improved performance and use of the same |
WO2017210188A1 (en) | 2016-05-31 | 2017-12-07 | Novozymes A/S | Stabilized liquid peroxide compositions |
WO2017210295A1 (en) | 2016-05-31 | 2017-12-07 | Danisco Us Inc. | Protease variants and uses thereof |
WO2017207762A1 (en) | 2016-06-03 | 2017-12-07 | Novozymes A/S | Subtilase variants and polynucleotides encoding same |
WO2017218861A1 (en) | 2016-06-17 | 2017-12-21 | The Procter & Gamble Company | Detergent composition |
EP3257931A1 (en) | 2016-06-17 | 2017-12-20 | The Procter and Gamble Company | Detergent composition |
EP4151726A1 (en) | 2016-06-17 | 2023-03-22 | Danisco US Inc | Protease variants and uses thereof |
WO2017219011A1 (en) | 2016-06-17 | 2017-12-21 | Danisco Us Inc | Protease variants and uses thereof |
WO2017220422A1 (en) | 2016-06-23 | 2017-12-28 | Novozymes A/S | Use of enzymes, composition and method for removing soil |
WO2018002261A1 (en) | 2016-07-01 | 2018-01-04 | Novozymes A/S | Detergent compositions |
WO2018007435A1 (en) | 2016-07-05 | 2018-01-11 | Novozymes A/S | Pectate lyase variants and polynucleotides encoding same |
WO2018007573A1 (en) | 2016-07-08 | 2018-01-11 | Novozymes A/S | Detergent compositions with galactanase |
EP3950941A2 (en) | 2016-07-13 | 2022-02-09 | Novozymes A/S | Dnase polypeptide variants |
WO2018011277A1 (en) | 2016-07-13 | 2018-01-18 | Novozymes A/S | Bacillus cibi dnase variants |
WO2018011276A1 (en) | 2016-07-13 | 2018-01-18 | The Procter & Gamble Company | Bacillus cibi dnase variants and uses thereof |
WO2018015295A1 (en) | 2016-07-18 | 2018-01-25 | Novozymes A/S | Lipase variants, polynucleotides encoding same and the use thereof |
EP4357453A2 (en) | 2016-07-18 | 2024-04-24 | Novozymes A/S | Lipase variants, polynucleotides encoding same and the use thereof |
EP3272767A1 (en) | 2016-07-21 | 2018-01-24 | Fornia BioSolutions, Inc. | G24 glucoamylase compositions and methods |
WO2018015303A1 (en) | 2016-07-21 | 2018-01-25 | Novozymes A/S | Serine protease variants and polynucleotides encoding same |
WO2018017105A1 (en) | 2016-07-21 | 2018-01-25 | Fornia Biosolutions, Inc. | G24 glucoamylase compositions and methods |
WO2018015304A1 (en) | 2016-07-21 | 2018-01-25 | Novozymes A/S | Serine protease variants and polynucleotides encoding same |
WO2018026384A1 (en) | 2016-08-05 | 2018-02-08 | Fornia Biosolutions, Inc. | 2g16 glucoamylase compositions |
EP3284805A1 (en) | 2016-08-17 | 2018-02-21 | The Procter & Gamble Company | Cleaning composition comprising enzymes |
WO2018034842A1 (en) | 2016-08-17 | 2018-02-22 | The Procter & Gamble Company | Cleaning composition comprising enzymes |
WO2018037061A1 (en) | 2016-08-24 | 2018-03-01 | Novozymes A/S | Xanthan lyase variants and polynucleotides encoding same |
WO2018037064A1 (en) | 2016-08-24 | 2018-03-01 | Henkel Ag & Co. Kgaa | Detergent compositions comprising xanthan lyase variants i |
WO2018037065A1 (en) | 2016-08-24 | 2018-03-01 | Henkel Ag & Co. Kgaa | Detergent composition comprising gh9 endoglucanase variants i |
WO2018037062A1 (en) | 2016-08-24 | 2018-03-01 | Novozymes A/S | Gh9 endoglucanase variants and polynucleotides encoding same |
WO2018060216A1 (en) | 2016-09-29 | 2018-04-05 | Novozymes A/S | Use of enzyme for washing, method for washing and warewashing composition |
WO2018060475A1 (en) | 2016-09-29 | 2018-04-05 | Novozymes A/S | Spore containing granule |
WO2018075430A1 (en) | 2016-10-17 | 2018-04-26 | Novozymes A/S | Methods of reducing foam during ethanol fermentation |
WO2018077938A1 (en) | 2016-10-25 | 2018-05-03 | Novozymes A/S | Detergent compositions |
WO2018083093A1 (en) | 2016-11-01 | 2018-05-11 | Novozymes A/S | Multi-core granules |
WO2018085524A2 (en) | 2016-11-07 | 2018-05-11 | Danisco Us Inc | Laundry detergent composition |
WO2018093285A1 (en) | 2016-11-18 | 2018-05-24 | Baltika Breweries - Part Of The Carlsberg Group | Method of producing a grain malt and the malt product obtained in this way |
WO2018098381A1 (en) | 2016-11-23 | 2018-05-31 | Novozymes A/S | Improved yeast for ethanol production |
WO2018098124A1 (en) | 2016-11-23 | 2018-05-31 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
WO2018102348A1 (en) * | 2016-11-29 | 2018-06-07 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2018099762A1 (en) | 2016-12-01 | 2018-06-07 | Basf Se | Stabilization of enzymes in compositions |
WO2018108865A1 (en) | 2016-12-12 | 2018-06-21 | Novozymes A/S | Use of polypeptides |
WO2018112123A1 (en) | 2016-12-15 | 2018-06-21 | Danisco Us Inc. | Polypeptides with endoglucanase activity and uses thereof |
WO2018118917A1 (en) | 2016-12-21 | 2018-06-28 | Danisco Us Inc. | Protease variants and uses thereof |
WO2018118950A1 (en) | 2016-12-21 | 2018-06-28 | Danisco Us Inc. | Bacillus gibsonii-clade serine proteases |
EP4212622A2 (en) | 2016-12-21 | 2023-07-19 | Danisco US Inc. | Bacillus gibsonii-clade serine proteases |
WO2018156705A1 (en) | 2017-02-24 | 2018-08-30 | Danisco Us Inc. | Compositions and methods for increased protein production in bacillus licheniformis |
EP4353828A2 (en) | 2017-02-24 | 2024-04-17 | Danisco US Inc. | Compositions and methods for increased protein production in bacillus licheniformis |
WO2018169750A1 (en) | 2017-03-15 | 2018-09-20 | Danisco Us Inc | Trypsin-like serine proteases and uses thereof |
WO2018184004A1 (en) | 2017-03-31 | 2018-10-04 | Danisco Us Inc | Alpha-amylase combinatorial variants |
WO2018177938A1 (en) | 2017-03-31 | 2018-10-04 | Novozymes A/S | Polypeptides having dnase activity |
WO2018183662A1 (en) | 2017-03-31 | 2018-10-04 | Danisco Us Inc | Delayed release enzyme formulations for bleach-containing detergents |
WO2018177936A1 (en) | 2017-03-31 | 2018-10-04 | Novozymes A/S | Polypeptides having dnase activity |
WO2018178061A1 (en) | 2017-03-31 | 2018-10-04 | Novozymes A/S | Polypeptides having rnase activity |
WO2018185048A1 (en) | 2017-04-03 | 2018-10-11 | Novozymes A/S | Recovery process |
WO2018185152A1 (en) | 2017-04-04 | 2018-10-11 | Novozymes A/S | Polypeptide compositions and uses thereof |
WO2018185181A1 (en) | 2017-04-04 | 2018-10-11 | Novozymes A/S | Glycosyl hydrolases |
WO2018185150A1 (en) | 2017-04-04 | 2018-10-11 | Novozymes A/S | Polypeptides |
EP3385361A1 (en) | 2017-04-05 | 2018-10-10 | Henkel AG & Co. KGaA | Detergent compositions comprising bacterial mannanases |
EP3385362A1 (en) | 2017-04-05 | 2018-10-10 | Henkel AG & Co. KGaA | Detergent compositions comprising fungal mannanases |
WO2018184767A1 (en) | 2017-04-05 | 2018-10-11 | Henkel Ag & Co. Kgaa | Detergent compositions comprising bacterial mannanases |
WO2018185285A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2018185280A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2018184818A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2018184816A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2018184873A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Detergent compositions and uses thereof |
WO2018184817A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2018185267A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
EP3626809A1 (en) | 2017-04-06 | 2020-03-25 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2018185269A1 (en) | 2017-04-06 | 2018-10-11 | Novozymes A/S | Cleaning compositions and uses thereof |
EP3967756A1 (en) | 2017-04-06 | 2022-03-16 | Novozymes A/S | Detergent compositions and uses thereof |
WO2018202846A1 (en) | 2017-05-05 | 2018-11-08 | Novozymes A/S | Compositions comprising lipase and sulfite |
EP3401385A1 (en) | 2017-05-08 | 2018-11-14 | Henkel AG & Co. KGaA | Detergent composition comprising polypeptide comprising carbohydrate-binding domain |
WO2018206178A1 (en) | 2017-05-08 | 2018-11-15 | Henkel Ag & Co. Kgaa | Detergent composition comprising polypeptide comprising carbohydrate-binding domain |
WO2018206535A1 (en) | 2017-05-08 | 2018-11-15 | Novozymes A/S | Carbohydrate-binding domain and polynucleotides encoding the same |
WO2018222990A1 (en) | 2017-06-02 | 2018-12-06 | Novozymes A/S | Improved yeast for ethanol production |
WO2018224544A1 (en) | 2017-06-08 | 2018-12-13 | Novozymes A/S | Compositions comprising polypeptides having cellulase activity and amylase activity, and uses thereof in cleaning and detergent compositions |
EP4015524A1 (en) | 2017-06-28 | 2022-06-22 | Novozymes A/S | Polypeptides having trehalase activity and polynucleotides encoding same |
WO2019005755A1 (en) | 2017-06-28 | 2019-01-03 | Novozymes A/S | Polypeptides having trehalase activity and polynucleotides encoding same |
WO2019006077A1 (en) | 2017-06-30 | 2019-01-03 | Danisco Us Inc | Low-agglomeration, enzyme-containing particles |
WO2019002356A1 (en) | 2017-06-30 | 2019-01-03 | Novozymes A/S | Enzyme slurry composition |
WO2019030165A1 (en) | 2017-08-08 | 2019-02-14 | Novozymes A/S | Polypeptides having trehalase activity and the use thereof in process of producing fermentation products |
WO2019036721A2 (en) | 2017-08-18 | 2019-02-21 | Danisco Us Inc | Alpha-amylase variants |
WO2019040412A1 (en) | 2017-08-23 | 2019-02-28 | Danisco Us Inc | Methods and compositions for efficient genetic modifications of bacillus licheniformis strains |
WO2019038059A1 (en) | 2017-08-24 | 2019-02-28 | Henkel Ag & Co. Kgaa | Detergent compositions comprising gh9 endoglucanase variants ii |
WO2019038057A1 (en) | 2017-08-24 | 2019-02-28 | Novozymes A/S | Xanthan lyase variants and polynucleotides encoding same |
WO2019038058A1 (en) | 2017-08-24 | 2019-02-28 | Novozymes A/S | Gh9 endoglucanase variants and polynucleotides encoding same |
WO2019038060A1 (en) | 2017-08-24 | 2019-02-28 | Henkel Ag & Co. Kgaa | Detergent composition comprising xanthan lyase variants ii |
WO2019046232A1 (en) | 2017-08-30 | 2019-03-07 | Novozymes A/S | Combined use of an endo-protease of the m35 family and an exo-protease of the s53 family in the fermentation of starch |
WO2019055261A1 (en) | 2017-09-13 | 2019-03-21 | Danisco Us Inc | Modified 5'-untranslated region (utr) sequences for increased protein production in bacillus |
WO2019055455A1 (en) | 2017-09-15 | 2019-03-21 | Novozymes A/S | Enzyme blends and processes for improving the nutritional quality of animal feed |
WO2019057758A1 (en) | 2017-09-20 | 2019-03-28 | Novozymes A/S | Use of enzymes for improving water absorption and/or whiteness |
WO2019057902A1 (en) | 2017-09-22 | 2019-03-28 | Novozymes A/S | Novel polypeptides |
WO2019063499A1 (en) | 2017-09-27 | 2019-04-04 | Novozymes A/S | Lipase variants and microcapsule compositions comprising such lipase variants |
WO2019067390A1 (en) | 2017-09-27 | 2019-04-04 | The Procter & Gamble Company | Detergent compositions comprising lipases |
WO2019068713A1 (en) | 2017-10-02 | 2019-04-11 | Novozymes A/S | Polypeptides having mannanase activity and polynucleotides encoding same |
WO2019068715A1 (en) | 2017-10-02 | 2019-04-11 | Novozymes A/S | Polypeptides having mannanase activity and polynucleotides encoding same |
WO2019070883A1 (en) | 2017-10-04 | 2019-04-11 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
WO2019076800A1 (en) | 2017-10-16 | 2019-04-25 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2019076834A1 (en) | 2017-10-16 | 2019-04-25 | Novozymes A/S | Low dusting granules |
WO2019076833A1 (en) | 2017-10-16 | 2019-04-25 | Novozymes A/S | Low dusting granules |
WO2019083831A1 (en) | 2017-10-23 | 2019-05-02 | Novozymes A/S | Processes for reducing lactic acid in a biofuel fermentation system |
WO2019081515A1 (en) | 2017-10-24 | 2019-05-02 | Novozymes A/S | Compositions comprising polypeptides having mannanase activity |
WO2019081724A1 (en) | 2017-10-27 | 2019-05-02 | Novozymes A/S | Dnase variants |
WO2019081721A1 (en) | 2017-10-27 | 2019-05-02 | Novozymes A/S | Dnase variants |
WO2019084349A1 (en) | 2017-10-27 | 2019-05-02 | The Procter & Gamble Company | Detergent compositions comprising polypeptide variants |
WO2019084350A1 (en) | 2017-10-27 | 2019-05-02 | The Procter & Gamble Company | Detergent compositions comprising polypeptide variants |
WO2019086530A1 (en) | 2017-11-01 | 2019-05-09 | Novozymes A/S | Polypeptides and compositions comprising such polypeptides |
DE102017125558A1 (en) | 2017-11-01 | 2019-05-02 | Henkel Ag & Co. Kgaa | CLEANING COMPOSITIONS CONTAINING DISPERSINE I |
WO2019086528A1 (en) | 2017-11-01 | 2019-05-09 | Novozymes A/S | Polypeptides and compositions comprising such polypeptides |
WO2019086521A1 (en) | 2017-11-01 | 2019-05-09 | Henkel Ag & Co. Kgaa | Cleaning compositions containing dispersins ii |
WO2019086532A1 (en) | 2017-11-01 | 2019-05-09 | Novozymes A/S | Methods for cleaning medical devices |
WO2019086520A1 (en) | 2017-11-01 | 2019-05-09 | Henkel Ag & Co. Kgaa | Cleaning compositions containing dispersins i |
DE102017125560A1 (en) | 2017-11-01 | 2019-05-02 | Henkel Ag & Co. Kgaa | CLEANSING COMPOSITIONS CONTAINING DISPERSINE III |
DE102017125559A1 (en) | 2017-11-01 | 2019-05-02 | Henkel Ag & Co. Kgaa | CLEANSING COMPOSITIONS CONTAINING DISPERSINE II |
WO2019086526A1 (en) | 2017-11-01 | 2019-05-09 | Henkel Ag & Co. Kgaa | Cleaning compositions containing dispersins iii |
WO2019089898A1 (en) | 2017-11-02 | 2019-05-09 | Danisco Us Inc | Freezing point depressed solid matrix compositions for melt granulation of enzymes |
WO2019105781A1 (en) | 2017-11-29 | 2019-06-06 | Basf Se | Storage-stable enzyme preparations, their production and use |
WO2019108599A1 (en) | 2017-11-29 | 2019-06-06 | Danisco Us Inc | Subtilisin variants having improved stability |
WO2019110462A1 (en) | 2017-12-04 | 2019-06-13 | Novozymes A/S | Lipase variants and polynucleotides encoding same |
WO2019113415A1 (en) | 2017-12-08 | 2019-06-13 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
US11384347B2 (en) | 2017-12-08 | 2022-07-12 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
US11326153B2 (en) | 2017-12-08 | 2022-05-10 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2019113413A1 (en) | 2017-12-08 | 2019-06-13 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2019125683A1 (en) | 2017-12-21 | 2019-06-27 | Danisco Us Inc | Enzyme-containing, hot-melt granules comprising a thermotolerant desiccant |
WO2019135868A1 (en) | 2018-01-03 | 2019-07-11 | Danisco Us Inc | Mutant and genetically modified bacillus cells and methods thereof for increased protein production |
WO2019148192A1 (en) | 2018-01-29 | 2019-08-01 | Novozymes A/S | Microorganisms with improved nitrogen utilization for ethanol production |
WO2019156670A1 (en) | 2018-02-08 | 2019-08-15 | Danisco Us Inc. | Thermally-resistant wax matrix particles for enzyme encapsulation |
WO2019154954A1 (en) | 2018-02-08 | 2019-08-15 | Novozymes A/S | Lipase variants and compositions thereof |
WO2019154952A1 (en) | 2018-02-08 | 2019-08-15 | Novozymes A/S | Lipase variants and compositions thereof |
WO2019154955A1 (en) | 2018-02-08 | 2019-08-15 | Novozymes A/S | Lipase variants and compositions thereof |
WO2019154951A1 (en) | 2018-02-08 | 2019-08-15 | Novozymes A/S | Lipases, lipase variants and compositions thereof |
WO2019161227A1 (en) | 2018-02-15 | 2019-08-22 | Novozymes A/S | Improved yeast for ethanol production |
WO2019162000A1 (en) | 2018-02-23 | 2019-08-29 | Henkel Ag & Co. Kgaa | Detergent composition comprising xanthan lyase and endoglucanase variants |
WO2019175240A1 (en) | 2018-03-13 | 2019-09-19 | Novozymes A/S | Microencapsulation using amino sugar oligomers |
WO2019180111A1 (en) | 2018-03-23 | 2019-09-26 | Novozymes A/S | Subtilase variants and compositions comprising same |
WO2019197318A1 (en) | 2018-04-09 | 2019-10-17 | Novozymes A/S | Polypeptides having alpha-amylase activity and polynucleotides encoding same |
WO2019201793A1 (en) | 2018-04-17 | 2019-10-24 | Novozymes A/S | Polypeptides comprising carbohydrate binding activity in detergent compositions and their use in reducing wrinkles in textile or fabric. |
WO2019201783A1 (en) | 2018-04-19 | 2019-10-24 | Novozymes A/S | Stabilized cellulase variants |
WO2019201785A1 (en) | 2018-04-19 | 2019-10-24 | Novozymes A/S | Stabilized cellulase variants |
WO2019206994A1 (en) | 2018-04-26 | 2019-10-31 | Basf Se | Lipase enzymes |
WO2019211143A1 (en) | 2018-05-03 | 2019-11-07 | Basf Se | Amylase enzymes |
US10808234B2 (en) | 2018-05-04 | 2020-10-20 | Fornia Biosolutions, Inc. | Variant amylase enzyme compositions and methods |
WO2019231944A2 (en) | 2018-05-31 | 2019-12-05 | Novozymes A/S | Processes for enhancing yeast growth and productivity |
WO2019238761A1 (en) | 2018-06-15 | 2019-12-19 | Basf Se | Water soluble multilayer films containing wash active chemicals and enzymes |
WO2019245704A1 (en) | 2018-06-19 | 2019-12-26 | Danisco Us Inc | Subtilisin variants |
WO2019245705A1 (en) | 2018-06-19 | 2019-12-26 | Danisco Us Inc | Subtilisin variants |
WO2020002604A1 (en) | 2018-06-28 | 2020-01-02 | Novozymes A/S | Detergent compositions and uses thereof |
WO2020002255A1 (en) | 2018-06-29 | 2020-01-02 | Novozymes A/S | Subtilase variants and compositions comprising same |
WO2020002608A1 (en) | 2018-06-29 | 2020-01-02 | Novozymes A/S | Detergent compositions and uses thereof |
WO2020007863A1 (en) | 2018-07-02 | 2020-01-09 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2020007875A1 (en) | 2018-07-03 | 2020-01-09 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2020008043A1 (en) | 2018-07-06 | 2020-01-09 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2020008024A1 (en) | 2018-07-06 | 2020-01-09 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2020014407A1 (en) | 2018-07-11 | 2020-01-16 | Novozymes A/S | Processes for producing fermentation products |
WO2020023411A1 (en) | 2018-07-25 | 2020-01-30 | Novozymes A/S | Enzyme-expressing yeast for ethanol production |
WO2020028443A1 (en) | 2018-07-31 | 2020-02-06 | Danisco Us Inc | Variant alpha-amylases having amino acid substitutions that lower the pka of the general acid |
WO2020047215A1 (en) | 2018-08-30 | 2020-03-05 | Danisco Us Inc | Enzyme-containing granules |
WO2020068486A1 (en) | 2018-09-27 | 2020-04-02 | Danisco Us Inc | Compositions for medical instrument cleaning |
WO2020070063A2 (en) | 2018-10-01 | 2020-04-09 | Novozymes A/S | Detergent compositions and uses thereof |
WO2020070209A1 (en) | 2018-10-02 | 2020-04-09 | Novozymes A/S | Cleaning composition |
WO2020070014A1 (en) | 2018-10-02 | 2020-04-09 | Novozymes A/S | Cleaning composition comprising anionic surfactant and a polypeptide having rnase activity |
WO2020070011A1 (en) | 2018-10-02 | 2020-04-09 | Novozymes A/S | Cleaning composition |
WO2020070009A1 (en) | 2018-10-02 | 2020-04-09 | Novozymes A/S | Endonuclease 1 ribonucleases for cleaning |
WO2020070249A1 (en) | 2018-10-03 | 2020-04-09 | Novozymes A/S | Cleaning compositions |
WO2020070199A1 (en) | 2018-10-03 | 2020-04-09 | Novozymes A/S | Polypeptides having alpha-mannan degrading activity and polynucleotides encoding same |
WO2020069915A1 (en) | 2018-10-05 | 2020-04-09 | Basf Se | Compounds stabilizing hydrolases in liquids |
WO2020069914A1 (en) | 2018-10-05 | 2020-04-09 | Basf Se | Compounds stabilizing amylases in liquids |
WO2020069913A1 (en) | 2018-10-05 | 2020-04-09 | Basf Se | Compounds stabilizing hydrolases in liquids |
WO2020076697A1 (en) | 2018-10-08 | 2020-04-16 | Novozymes A/S | Enzyme-expressing yeast for ethanol production |
WO2020074545A1 (en) | 2018-10-11 | 2020-04-16 | Novozymes A/S | Cleaning compositions and uses thereof |
WO2020077331A2 (en) | 2018-10-12 | 2020-04-16 | Danisco Us Inc | Alpha-amylases with mutations that improve stability in the presence of chelants |
EP3647398A1 (en) | 2018-10-31 | 2020-05-06 | Henkel AG & Co. KGaA | Cleaning compositions containing dispersins v |
EP3647397A1 (en) | 2018-10-31 | 2020-05-06 | Henkel AG & Co. KGaA | Cleaning compositions containing dispersins iv |
WO2020088958A1 (en) | 2018-10-31 | 2020-05-07 | Henkel Ag & Co. Kgaa | Cleaning compositions containing dispersins v |
WO2020088957A1 (en) | 2018-10-31 | 2020-05-07 | Henkel Ag & Co. Kgaa | Cleaning compositions containing dispersins iv |
WO2020112599A1 (en) | 2018-11-28 | 2020-06-04 | Danisco Us Inc | Subtilisin variants having improved stability |
WO2020114968A1 (en) | 2018-12-03 | 2020-06-11 | Novozymes A/S | Powder detergent compositions |
WO2020114965A1 (en) | 2018-12-03 | 2020-06-11 | Novozymes A/S | LOW pH POWDER DETERGENT COMPOSITION |
WO2020127775A1 (en) | 2018-12-21 | 2020-06-25 | Novozymes A/S | Detergent pouch comprising metalloproteases |
WO2020131252A1 (en) | 2018-12-21 | 2020-06-25 | Fornia Biosolutions, Inc. | Variant g6p g7p glucoamylase compositions and methods |
WO2020127796A2 (en) | 2018-12-21 | 2020-06-25 | Novozymes A/S | Polypeptides having peptidoglycan degrading activity and polynucleotides encoding same |
EP3677676A1 (en) | 2019-01-03 | 2020-07-08 | Basf Se | Compounds stabilizing amylases in liquids |
WO2020160126A1 (en) | 2019-01-31 | 2020-08-06 | Novozymes A/S | Polypeptides having xylanase activity and use thereof for improving the nutritional quality of animal feed |
WO2020169564A1 (en) | 2019-02-20 | 2020-08-27 | Basf Se | Industrial fermentation process for bacillus using defined medium and trace element feed |
WO2020169563A1 (en) | 2019-02-20 | 2020-08-27 | Basf Se | Industrial fermentation process for bacillus using defined medium and magnesium feed |
WO2020178102A1 (en) | 2019-03-01 | 2020-09-10 | Novozymes A/S | Detergent compositions comprising two proteases |
EP3702452A1 (en) | 2019-03-01 | 2020-09-02 | Novozymes A/S | Detergent compositions comprising two proteases |
WO2020187883A1 (en) | 2019-03-18 | 2020-09-24 | Novozymes A/S | Polypeptides having pullulanase activity suitable for use in liquefaction |
WO2020188095A1 (en) | 2019-03-21 | 2020-09-24 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2020193532A1 (en) | 2019-03-25 | 2020-10-01 | Basf Se | Cleaning composition having amylase enzymes |
WO2020193534A2 (en) | 2019-03-25 | 2020-10-01 | Basf Se | Amylase enzymes |
WO2020193535A2 (en) | 2019-03-25 | 2020-10-01 | Basf Se | Amylase enzymes |
WO2020206058A1 (en) | 2019-04-02 | 2020-10-08 | Novozymes A/S | Process for producing a fermentation product |
WO2020201403A1 (en) | 2019-04-03 | 2020-10-08 | Novozymes A/S | Polypeptides having beta-glucanase activity, polynucleotides encoding same and uses thereof in cleaning and detergent compositions |
WO2020207944A1 (en) | 2019-04-10 | 2020-10-15 | Novozymes A/S | Polypeptide variants |
WO2020208056A1 (en) | 2019-04-12 | 2020-10-15 | Novozymes A/S | Stabilized glycoside hydrolase variants |
WO2020229480A1 (en) | 2019-05-14 | 2020-11-19 | Basf Se | Compounds stabilizing hydrolases in liquids |
WO2020242858A1 (en) | 2019-05-24 | 2020-12-03 | Danisco Us Inc | Subtilisin variants and methods of use |
WO2020247582A1 (en) | 2019-06-06 | 2020-12-10 | Danisco Us Inc | Methods and compositions for cleaning |
WO2020249546A1 (en) | 2019-06-13 | 2020-12-17 | Basf Se | Method of recovering a protein from fermentation broth using a divalent cation |
WO2021001400A1 (en) | 2019-07-02 | 2021-01-07 | Novozymes A/S | Lipase variants and compositions thereof |
WO2021004830A1 (en) | 2019-07-05 | 2021-01-14 | Basf Se | Industrial fermentation process for microbial cells using a fed-batch pre-culture |
WO2021009067A1 (en) | 2019-07-12 | 2021-01-21 | Novozymes A/S | Enzymatic emulsions for detergents |
WO2021021458A1 (en) | 2019-07-26 | 2021-02-04 | Novozymes A/S | Microorganisms with improved nitrogen transport for ethanol production |
WO2021026201A1 (en) | 2019-08-05 | 2021-02-11 | Novozymes A/S | Enzyme blends and processes for producing a high protein feed ingredient from a whole stillage byproduct |
WO2021025872A1 (en) | 2019-08-06 | 2021-02-11 | Novozymes A/S | Fusion proteins for improved enzyme expression |
WO2021030267A1 (en) | 2019-08-14 | 2021-02-18 | Danisco Us Inc | Compositions and methods for increased protein production in bacillus licheniformis |
WO2021032881A1 (en) | 2019-08-22 | 2021-02-25 | Basf Se | Amylase variants |
WO2021037895A1 (en) | 2019-08-27 | 2021-03-04 | Novozymes A/S | Detergent composition |
WO2021037878A1 (en) | 2019-08-27 | 2021-03-04 | Novozymes A/S | Composition comprising a lipase |
WO2021055395A1 (en) | 2019-09-16 | 2021-03-25 | Novozymes A/S | Polypeptides having beta-glucanase activity and polynucleotides encoding same |
WO2021053127A1 (en) | 2019-09-19 | 2021-03-25 | Novozymes A/S | Detergent composition |
WO2021064068A1 (en) | 2019-10-03 | 2021-04-08 | Novozymes A/S | Polypeptides comprising at least two carbohydrate binding domains |
WO2021074430A1 (en) | 2019-10-18 | 2021-04-22 | Basf Se | Storage-stable hydrolase containing liquids |
WO2021080948A2 (en) | 2019-10-24 | 2021-04-29 | Danisco Us Inc | Variant maltopentaose/maltohexaose-forming alpha-amylases |
WO2021096857A1 (en) | 2019-11-11 | 2021-05-20 | Danisco Us Inc | Compositions and methods for enhanced protein production in bacillus cells |
WO2021105336A1 (en) | 2019-11-29 | 2021-06-03 | Basf Se | Compositions comprising polymer and enzyme |
WO2021115912A1 (en) | 2019-12-09 | 2021-06-17 | Basf Se | Formulations comprising a hydrophobically modified polyethyleneimine and one or more enzymes |
WO2021119304A1 (en) | 2019-12-10 | 2021-06-17 | Novozymes A/S | Microorganism for improved pentose fermentation |
WO2021126966A1 (en) | 2019-12-16 | 2021-06-24 | Novozymes A/S | Processes for producing fermentation products |
WO2021123307A2 (en) | 2019-12-20 | 2021-06-24 | Novozymes A/S | Polypeptides having proteolytic activity and use thereof |
WO2021121394A1 (en) | 2019-12-20 | 2021-06-24 | Novozymes A/S | Stabilized liquid boron-free enzyme compositions |
WO2021122118A1 (en) | 2019-12-20 | 2021-06-24 | Henkel Ag & Co. Kgaa | Cleaning compositions comprising dispersins vi |
WO2021122117A1 (en) | 2019-12-20 | 2021-06-24 | Henkel Ag & Co. Kgaa | Cleaning composition coprising a dispersin and a carbohydrase |
WO2021122120A2 (en) | 2019-12-20 | 2021-06-24 | Henkel Ag & Co. Kgaa | Cleaning compositions comprising dispersins viii |
WO2021146411A1 (en) | 2020-01-15 | 2021-07-22 | Danisco Us Inc | Compositions and methods for enhanced protein production in bacillus licheniformis |
WO2021151536A1 (en) | 2020-01-29 | 2021-08-05 | Unilever Ip Holdings B.V. | Laundry detergent product |
WO2021152123A1 (en) | 2020-01-31 | 2021-08-05 | Novozymes A/S | Mannanase variants and polynucleotides encoding same |
WO2021152120A1 (en) | 2020-01-31 | 2021-08-05 | Novozymes A/S | Mannanase variants and polynucleotides encoding same |
WO2021163011A2 (en) | 2020-02-10 | 2021-08-19 | Novozymes A/S | Alpha-amylase variants and polynucleotides encoding same |
WO2021163030A2 (en) | 2020-02-10 | 2021-08-19 | Novozymes A/S | Polypeptides having alpha-amylase activity and polynucleotides encoding same |
EP3892708A1 (en) | 2020-04-06 | 2021-10-13 | Henkel AG & Co. KGaA | Cleaning compositions comprising dispersin variants |
WO2021204838A1 (en) | 2020-04-08 | 2021-10-14 | Novozymes A/S | Carbohydrate binding module variants |
WO2021214059A1 (en) | 2020-04-21 | 2021-10-28 | Novozymes A/S | Cleaning compositions comprising polypeptides having fructan degrading activity |
WO2021224389A1 (en) | 2020-05-07 | 2021-11-11 | Novozymes A/S | Medical cleaning composition, use and method of cleaning |
EP3907271A1 (en) | 2020-05-07 | 2021-11-10 | Novozymes A/S | Cleaning composition, use and method of cleaning |
WO2021239818A1 (en) | 2020-05-26 | 2021-12-02 | Novozymes A/S | Subtilase variants and compositions comprising same |
WO2021259099A1 (en) | 2020-06-24 | 2021-12-30 | Novozymes A/S | Use of cellulases for removing dust mite from textile |
EP3936593A1 (en) | 2020-07-08 | 2022-01-12 | Henkel AG & Co. KGaA | Cleaning compositions and uses thereof |
WO2022008387A1 (en) | 2020-07-08 | 2022-01-13 | Henkel Ag & Co. Kgaa | Cleaning compositions and uses thereof |
WO2022008732A1 (en) | 2020-07-10 | 2022-01-13 | Basf Se | Enhancing the activity of antimicrobial preservatives |
WO2022023250A1 (en) | 2020-07-27 | 2022-02-03 | Unilever Ip Holdings B.V. | Use of an enzyme and surfactant for inhibiting microorganisms |
WO2022043321A2 (en) | 2020-08-25 | 2022-03-03 | Novozymes A/S | Variants of a family 44 xyloglucanase |
WO2022047149A1 (en) | 2020-08-27 | 2022-03-03 | Danisco Us Inc | Enzymes and enzyme compositions for cleaning |
WO2022043547A1 (en) | 2020-08-28 | 2022-03-03 | Novozymes A/S | Protease variants with improved solubility |
WO2022043563A1 (en) | 2020-08-28 | 2022-03-03 | Novozymes A/S | Polyester degrading protease variants |
WO2022058322A1 (en) | 2020-09-15 | 2022-03-24 | Novozymes A/S | Animal feed comprising insects or insect meal |
WO2022063699A1 (en) | 2020-09-22 | 2022-03-31 | Basf Se | Improved combination of protease and protease inhibitor with secondary enzyme |
WO2022063698A1 (en) | 2020-09-22 | 2022-03-31 | Basf Se | Liquid composition comprising peptide aldehyde |
WO2022074037A2 (en) | 2020-10-07 | 2022-04-14 | Novozymes A/S | Alpha-amylase variants |
WO2022084303A2 (en) | 2020-10-20 | 2022-04-28 | Novozymes A/S | Use of polypeptides having dnase activity |
WO2022090320A1 (en) | 2020-10-28 | 2022-05-05 | Novozymes A/S | Use of lipoxygenase |
WO2022090361A2 (en) | 2020-10-29 | 2022-05-05 | Novozymes A/S | Lipase variants and compositions comprising such lipase variants |
WO2022090564A1 (en) | 2020-11-02 | 2022-05-05 | Novozymes A/S | Glucoamylase variants and polynucleotides encoding same |
WO2022103725A1 (en) | 2020-11-13 | 2022-05-19 | Novozymes A/S | Detergent composition comprising a lipase |
WO2022106400A1 (en) | 2020-11-18 | 2022-05-27 | Novozymes A/S | Combination of immunochemically different proteases |
WO2022106404A1 (en) | 2020-11-18 | 2022-05-27 | Novozymes A/S | Combination of proteases |
EP4015629A1 (en) | 2020-12-18 | 2022-06-22 | Basf Se | Polymer mixtures for increasing stability and performance of hydrolase-containing detergents |
WO2022157311A1 (en) | 2021-01-22 | 2022-07-28 | Novozymes A/S | Liquid enzyme composition with sulfite scavenger |
EP4032966A1 (en) | 2021-01-22 | 2022-07-27 | Novozymes A/S | Liquid enzyme composition with sulfite scavenger |
WO2022162043A1 (en) | 2021-01-28 | 2022-08-04 | Novozymes A/S | Lipase with low malodor generation |
WO2022165107A1 (en) | 2021-01-29 | 2022-08-04 | Danisco Us Inc | Compositions for cleaning and methods related thereto |
WO2022167251A1 (en) | 2021-02-04 | 2022-08-11 | Henkel Ag & Co. Kgaa | Detergent composition comprising xanthan lyase and endoglucanase variants with improved stability |
EP4039806A1 (en) | 2021-02-04 | 2022-08-10 | Henkel AG & Co. KGaA | Detergent composition comprising xanthan lyase and endoglucanase variants with im-proved stability |
WO2022171780A2 (en) | 2021-02-12 | 2022-08-18 | Novozymes A/S | Alpha-amylase variants |
WO2022171872A1 (en) | 2021-02-12 | 2022-08-18 | Novozymes A/S | Stabilized biological detergents |
WO2022178432A1 (en) | 2021-02-22 | 2022-08-25 | Danisco Us Inc. | Methods and compositions for producing proteins of interest in pigment deficient bacillus cells |
EP4047088A1 (en) | 2021-02-22 | 2022-08-24 | Basf Se | Amylase variants |
WO2022175435A1 (en) | 2021-02-22 | 2022-08-25 | Basf Se | Amylase variants |
WO2022189521A1 (en) | 2021-03-12 | 2022-09-15 | Novozymes A/S | Polypeptide variants |
WO2022194673A1 (en) | 2021-03-15 | 2022-09-22 | Novozymes A/S | Dnase variants |
EP4060036A1 (en) | 2021-03-15 | 2022-09-21 | Novozymes A/S | Polypeptide variants |
WO2022194668A1 (en) | 2021-03-15 | 2022-09-22 | Novozymes A/S | Polypeptide variants |
WO2022199418A1 (en) | 2021-03-26 | 2022-09-29 | Novozymes A/S | Detergent composition with reduced polymer content |
WO2022261003A1 (en) | 2021-06-07 | 2022-12-15 | Novozymes A/S | Engineered microorganism for improved ethanol fermentation |
WO2022268885A1 (en) | 2021-06-23 | 2022-12-29 | Novozymes A/S | Alpha-amylase polypeptides |
WO2023278297A1 (en) | 2021-06-30 | 2023-01-05 | Danisco Us Inc | Variant lipases and uses thereof |
WO2023023644A1 (en) | 2021-08-20 | 2023-02-23 | Danisco Us Inc. | Polynucleotides encoding novel nucleases, compositions thereof and methods thereof for eliminating dna from protein preparations |
WO2023034486A2 (en) | 2021-09-03 | 2023-03-09 | Danisco Us Inc. | Laundry compositions for cleaning |
WO2023039270A2 (en) | 2021-09-13 | 2023-03-16 | Danisco Us Inc. | Bioactive-containing granules |
WO2023114939A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Subtilisin variants and methods of use |
WO2023114988A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Variant maltopentaose/maltohexaose-forming alpha-amylases |
WO2023114932A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Subtilisin variants and methods of use |
WO2023114936A2 (en) | 2021-12-16 | 2023-06-22 | Danisco Us Inc. | Subtilisin variants and methods of use |
WO2023116569A1 (en) | 2021-12-21 | 2023-06-29 | Novozymes A/S | Composition comprising a lipase and a booster |
WO2023118015A1 (en) | 2021-12-21 | 2023-06-29 | Basf Se | Environmental attributes for care composition ingredients |
EP4206309A1 (en) | 2021-12-30 | 2023-07-05 | Novozymes A/S | Protein particles with improved whiteness |
WO2023126254A1 (en) | 2021-12-30 | 2023-07-06 | Novozymes A/S | Protein particles with improved whiteness |
EP4234664A1 (en) | 2022-02-24 | 2023-08-30 | Evonik Operations GmbH | Composition comprising glucolipids and enzymes |
WO2023168234A1 (en) | 2022-03-01 | 2023-09-07 | Danisco Us Inc. | Enzymes and enzyme compositions for cleaning |
WO2023165507A1 (en) | 2022-03-02 | 2023-09-07 | Novozymes A/S | Use of xyloglucanase for improvement of sustainability of detergents |
WO2023165950A1 (en) | 2022-03-04 | 2023-09-07 | Novozymes A/S | Dnase variants and compositions |
WO2023194204A1 (en) | 2022-04-08 | 2023-10-12 | Novozymes A/S | Hexosaminidase variants and compositions |
WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
WO2023233028A1 (en) | 2022-06-03 | 2023-12-07 | Unilever Ip Holdings B.V. | Laundry detergent product |
WO2023250301A1 (en) | 2022-06-21 | 2023-12-28 | Danisco Us Inc. | Methods and compositions for cleaning comprising a polypeptide having thermolysin activity |
WO2023247348A1 (en) | 2022-06-21 | 2023-12-28 | Novozymes A/S | Mannanase variants and polynucleotides encoding same |
WO2023247664A2 (en) | 2022-06-24 | 2023-12-28 | Novozymes A/S | Lipase variants and compositions comprising such lipase variants |
WO2024012894A1 (en) | 2022-07-15 | 2024-01-18 | Basf Se | Alkanolamine formates for enzyme stabilization in liquid formulations |
WO2024033135A2 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Amylase variants |
WO2024033136A1 (en) | 2022-08-11 | 2024-02-15 | Basf Se | Amylase variants |
WO2024050339A1 (en) | 2022-09-02 | 2024-03-07 | Danisco Us Inc. | Mannanase variants and methods of use |
WO2024050343A1 (en) | 2022-09-02 | 2024-03-07 | Danisco Us Inc. | Subtilisin variants and methods related thereto |
WO2024050346A1 (en) | 2022-09-02 | 2024-03-07 | Danisco Us Inc. | Detergent compositions and methods related thereto |
Also Published As
Publication number | Publication date |
---|---|
ES2436066T3 (en) | 2013-12-26 |
EP1023439A1 (en) | 2000-08-02 |
AU9434398A (en) | 1999-05-03 |
ATE423192T1 (en) | 2009-03-15 |
ES2322825T3 (en) | 2009-06-29 |
US6187576B1 (en) | 2001-02-13 |
DE69840577D1 (en) | 2009-04-02 |
EP2302027A2 (en) | 2011-03-30 |
EP2302027A3 (en) | 2011-06-29 |
DK2302027T3 (en) | 2013-12-02 |
EP2302027B1 (en) | 2013-08-28 |
CA2305191C (en) | 2011-09-27 |
CA2745783C (en) | 2016-05-24 |
CA2305191A1 (en) | 1999-04-22 |
KR20010015754A (en) | 2001-02-26 |
ES2536878T3 (en) | 2015-05-29 |
EP2206768A2 (en) | 2010-07-14 |
JP2001520006A (en) | 2001-10-30 |
JP4358431B2 (en) | 2009-11-04 |
DK2206768T3 (en) | 2015-06-29 |
EP2206768B1 (en) | 2015-04-01 |
CA2745783A1 (en) | 1999-04-22 |
EP2206768A3 (en) | 2010-10-06 |
EP1023439B1 (en) | 2009-02-18 |
AR017331A1 (en) | 2001-09-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP2302027B1 (en) | Alpha-amylase mutants | |
EP1131418B1 (en) | Alpha-amylase variants | |
US7625737B2 (en) | Alpha-amylase mutants | |
US7713723B1 (en) | Alpha-amylase mutants with altered properties | |
US8465957B2 (en) | Alpha-amylase variants | |
WO2001088107A2 (en) | Alpha-amylase variants with altered 1,6-activity | |
US20020068352A1 (en) | Alpha-amylase variants with altered 1, 6-activity |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GD GE GH GM HR HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH GM KE LS MW SD SZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 1998947417 Country of ref document: EP |
|
ENP | Entry into the national phase |
Ref document number: 2305191 Country of ref document: CA Ref country code: CA Ref document number: 2305191 Kind code of ref document: A Format of ref document f/p: F |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1020007003941 Country of ref document: KR |
|
WWP | Wipo information: published in national office |
Ref document number: 1998947417 Country of ref document: EP |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWP | Wipo information: published in national office |
Ref document number: 1020007003941 Country of ref document: KR |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 1020007003941 Country of ref document: KR |