WO1999003886A1

WO1999003886A1 - Tropoelastin derivatives

Info

Publication number: WO1999003886A1
Application number: PCT/AU1998/000564
Authority: WO
Inventors: Anthony Steven Weiss
Original assignee: The University Of Sydney
Priority date: 1997-07-18
Filing date: 1998-07-17
Publication date: 1999-01-28
Also published as: AUPO811797A0; US8710015B2; CA2296841A1; NZ502026A; US20080039397A1; US20120130046A1; US7803577B2; DE69839939D1; EP1978031A3; KR20010021999A; KR100636948B1; EP1007555B1; ATE406382T1; EP1978031A2; CA2296841C; JP2001510032A; EP1007555A4; US20110021752A1; EP1007555A1

Abstract

The invention relates to derivatives of tropoelastin and variants of those derivatives. The invention further provides expression products and hybrid molecules of the derivatives and variants of the invention. The invention further provides methods for the production of the derivatives, variants, expression products and hybrid molecules. Further provided are formulations, cross-linked structures and implants comprising the derivatives, variants, expression products and hybrid molecules of the invention. Further provided are uses of the derivatives, variants, expression products and hybrid molecules of the invention.

Description

TROPOELASTIN DERIVATIVES

TECHNICAL FIELD The present invention relates to derivatives of human tropoelastin and variants thereof, to genetic constructs encoding the amino acid sequences of the derivatives and variants and to uses of the derivatives and variants . In particular, the derivatives of the present invention have elastin-like properties or macro-molecular binding properties.

BACKGROUND ART There are various forms of tropoelastin that typically appear to consist of two types of alternating domains: those rich in hydrophobic amino acids

(responsible for the elastic properties) and those rich in lysine residues (responsible for cross-link formation) . Hydrophobic and cross-linking domains are encoded in separate exons (Indik et al 1987). The 26 A region of human tropoelastin is unique amongst tropoelastin domains in that, due to the absence of lysine, this region does not participate in elastin cross-link formation. Furthermore, this region is a serine-rich domain and lacks hydrophobic stretches, indicating that it is unlikely to contribute to the elasticity of tropoelastin. There is otherwise limited information on the structure and functional relationships of the 26 A region (Bedell-Hogan et al . , 1993).

The gene for tropoelastin is believed to be present as a single copy in the mammalian genome, and is expressed in the form of multiple transcripts, distinguished by alternative splicing of the pre-mRNA (Indik et al , 1990; Oliver et al , 1987) . Modest expression of a natural human tropoelastin sequence has been achieved by Indik et al (1990) using cDNA, providing free polypeptide which unfortunately was unstable.

Expression of substantial amounts of human tropoelastin using synthetic polynucleotides is reported in W094/14958. In particular, a construct, SHEL, providing substantial amounts of full length human tropoelastin is described.

DESCRIPTION OF THE INVENTION

In the specification and claims, "derivatives of human tropoelastin" or "tropoelastin derivatives" means novel peptides, polypeptides or proteins which contain amino acid sequences derived from the native amino acid sequences of human tropoelastin molecules. The amino acid sequences of the derivatives of human tropoelastin may be derived from any of the amino acid sequences of the isoforms of human tropoelastin. Derivatives of human tropoelastin are distinguished from human tropoelastin molecules in that the amino acid sequences of derivatives are altered with respect to native tropoelastin sequences by substitution, addition or deletion of residues, or a combination of these alterations, in derivative amino acid sequences . In a first aspect, the present invention provides derivatives of human tropoelastin which have elastin-like properties. Elastin-like properties are a combination of elastic properties, including the phenomenon of recoil following molecular distention under appropriate conditions, and the ability to be cross-linked to other elastin molecules and/or other elastin-like molecules.

In a second aspect, the present invention provides derivatives of human tropoelastin which have macro- molecular binding properties including the ability to bind glycosaminoglycans .

In a third aspect, the present invention provides derivatives of human tropoelastin which have elastin-like properties and macro-molecular binding properties .

The present invention further provides amino acid sequence variants of the derivatives of the invention. In the specification and claims "variants" means amino acid sequences which retain the properties of the corresponding derivative of human tropoelastin, for example, elastin- like properties or macro-molecular binding properties, or a combination of elastin-like properties and macro- molecular binding properties, and have an amino acid sequence which is homologous with the amino acid sequence of the corresponding derivative. For the purposes of this description, "homology" between the amino acid sequence of a particular derivative of human tropoelastin and another amino acid sequence connotes a likeness short of identity, indicative of a derivation of one sequence from the other. In particular, an amino acid sequence is homologous to a derivative of human tropoelastin if the alignment of that amino acid sequence with the sequence of the derivative of human tropoelastin reveals a similarity of about 65% over any 20 amino acid stretch or over any repetitive element of the molecules shorter than 20 amino acids in length. Such a sequence comparison can be performed via known algorithims, such as that of Lipman and Pearson (1985) . Similarity is observed between amino acids where those amino acids have a side chain which confers a similar chemical property in the same chemical environment. For example, threonine and serine are similar amino acids; aspartic acid and glutamic acid are similar amino acids; valine, leucine and isoleucine are similar amino acids etc. Thus, an amino acid sequence may be considered homologous with the amino acid sequence of a human tropoelastin derivative because the alignment of those sequences reveals a similarity of 65%, although at each amino acid position in the aligned sequences, none of the residues are identical. Inasmuch as the present invention provides derivatives of human tropoelastin and amino acid sequence variants of those derivatives, the invention thus extends to amino acid sequence variants incorporating amino acid sequences of non-human tropoelastins . Amino acid sequence variants which are non-human tropoelastin derivatives, or are based all, or in part, on non-human tropoelastin derivatives retain properties of the corresponding derivative of non-human tropoelastin, for example, elastin-like properties or macro-molecular binding properties, or a combination of elastin-like properties and macro-molecular binding properties, and have an amino acid sequence which is homologous with the amino acid sequence of the corresponding human derivative. The variants of the invention also include variants of the non-human tropoelastin derivatives, or of derivatives based on the non-human tropoelastin derivatives. "Homology" between the amino acid sequence of a particular derivative of non-human tropoelastin and another amino acid sequence connotes a likeness short of identity, indicative of a derivation of one sequence from the other. In particular, an amino acid sequence is homologous to a derivative of non-human tropoelastin if the alignment of that amino acid sequence with the sequence of the derivative of non-human tropoelastin reveals a similarity of about 65% over any 20 amino acid stretch or over any repetitive element of the molecules shorter than 20 amino acids in length. The skilled addressee will understand that species that are substantially phylogenetically related to humans express tropoelastin molecules which consist of amino acid sequences with homology to human tropoelastin amino acid sequences. Indeed, amino acid sequences of non-human tropoelastins have been determined, including the amino acid sequences of chick tropoelastin, bovine tropoelastin and rat tropoelastin (Bressan et al . 1987, Raju et al . 1987, Pierce et al . 1992) and over multiple regions, these are homologous with the human tropoelastin amino acid sequences. The skilled addressee will recognise therefore, that derivatives of human tropoelastin and amino acid sequence variants of those derivatives will necessarily encompass corresponding tropoelastin amino acid sequences from these and other non-human species . The present invention provides a tropoelastin derivative comprising the amino acid sequence of SHELδmodified (SEQ ID NO:5). The amino acid sequence of SHELδmodified and the alignment of that amino acid sequence with the human tropoelastin sequence is shown in Figure 5.

The invention also provides an amino acid sequence variant of the derivative comprising the amino acid sequence of SHELδmodified.

The invention also provides a polynucleotide encoding a tropoelastin derivative comprising the amino acid sequence of SHELδmodified. The nucleotide sequence encoding SHELδmodified is shown in Figure 3 (SEQ ID NO:

4). Preferably the polynucleotide comprises the nucleotide sequence which corresponds to SHELδmodified shown in Figure 3.

The invention also provides a polynucleotide encoding an amino acid sequence variant of the derivative SHELδmodified.

The present invention further provides a synthetic polynucleotide encoding a tropoelastin derivative comprising the amino acid sequence of SHELδ26A (SEQ ID NO: 3) . A synthetic polynucleotide is a molecule which comprises a nucleotide sequence that contains silent mutations with respect to the corresponding native polynucleotide molecule. The silent mutations enhance the expression of the synthetic polynucleotide. The amino acid sequence of SHELδ26A and the alignment of that amino acid sequence with the human tropoelastin sequence is shown in Figure 2. The SHELδ26A derivative excludes the SHEL coding sequence corresponding to exon 26A. Preferably the synthetic polynucleotide comprises the sequence shown in Figure 1 (SEQ ID NO:l) from nucleotide position 1 to 1676 contiguous with nucleotide position 1775 to 2210.

The invention also provides a polynucleotide encoding an amino acid sequence variant of the derivative SHELδ26A. The invention also provides an amino acid sequence variant of the derivative comprising the amino acid sequence of SHELδ26A.

The present inventor has, for the first time, shown that the region encoded by exon 26A (peptide 26A) of the tropoelastin gene binds glycosammoglycans (GAGs) (Figure 6A and B) . GAGs are macro-molecules particularly associated with the extracellular environment. These molecules play an important role in the architecture and mechanical properties of connective tissues and mediate interactions with and availability of other molecules.

Thus, the present invention provides a tropoelastin derivative comprising the amino acid sequence of peptide 26A. Peptide 26A has the amino acid sequence: GADEGVRRSLSPELREGDPSSSQHLPSTPSSPRV (SEQ ID NO: 12) or GADEGVRRSLSPELREGDPSSSQHLPSTPSSPRF (SEQ ID NO : 13 ) .

The present invention also provides an amino acid sequence variant of the derivative comprising the amino acid sequence of peptide 26A.

The invention also provides a polynucleotide encoding a tropoelastin derivative comprising the amino acid sequence of peptide 26A. Preferably the polynucleotide comprises the nucleotide sequence shown in Figure 1 (SEQ ID NO: 1) from nucleotide position 1687 to 1778. Preferably the 3 ' terminal codon is GTT (which encodes V) or TTT (which encodes F) .

The invention also provides a polynucleotide encoding an amino acid sequence variant of the derivative comprising the amino acid sequence of peptide 26A.

In appreciating the GAG binding property of peptide 26A, the present inventor envisages the generation of novel subsets of hybrid molecules, comprising biological polymers which are linked to peptide 26A, wherein the peptide 26A imparts GAG binding activity to the polymer. In particular, the present inventor has recognised that the deletion or insertion of the peptide 26A amino acid sequence, or a variant of that amino acid sequence will alter GAG binding activity. Thus, the present invention relates to tropoelastin derivatives in which full length or partial length tropoelastin molecules have been modified by the addition of one or more exon 26A regions to enhance interactions with GAGs. Moreover, the invention relates to site directed modification of the amino acid sequence of peptide 26A so as to generate variants of the peptide 26A amino acid sequence which have altered affinity or altered specificity for GAGs. Tropoelastin derivatives or variants of the derivatives which contain altered GAG binding activity may be uncross- linked or cross-linked.

In another aspect, the invention provides a hybrid molecule. In the specification and claims, "hybrid molecule" means a molecule comprising a biological polymer which is linked to a tropoelastin derivative comprising the amino acid sequence of peptide 26A or an amino acid sequence variant of a derivative comprising the amino acid sequence of peptide 26A. Preferably the biological polymer is a protein. More preferably the protein is selected from the group consisting of growth factors, cytokines and antibodies. Alternatively the biological polymer is selected from the group consisting of lipids, sugars or nucleic acids.

In one embodiment, and where the biological polymer is a protein, the hybrid molecule is produced by recombinant DNA techniques, including for example the construction of a nucleotide sequence which encodes the biological polymer and the tropoelastin derivative comprising the amino acid sequence of peptide 26A, or the amino acid sequence variant of a derivative comprising the amino acid sequence of peptide 26 A, in a single open reading frame. Alternatively, the hybrid molecule may be produced synthetically by solid phase peptide synthesis, including, for example the methods of synthesis disclosed in Merrifield (1963) or Knorr et al . (1989). Examples of peptide synthesis also include the synthesis methods used by peptide synthesisers of Perkin Elmer/Applied Biosystems, CA, US.

In another aspect, the invention provides a polynucleotide sequence encoding a hybrid molecule of the invention.

In another aspect, the invention provides a hybrid molecule which comprises a synthetic polymer which is linked in a tropoelastin derivative comprising the amino acid sequence of peptide 26A, or an amino acid sequence variant of the derivative comprising the amino acid sequence of peptide 26A.

The invention further provides a method of imparting or enhancing GAG binding activity to a biological polymer comprising the step of linking a tropoelastin derivative comprising the amino acid sequence of peptide 26A, or an amino acid sequence variant of peptide 26A with the biological polymer. Preferably the biological polymer is a protein.

The invention further provides a method of deleting or reducing GAG binding activity from a biological polymer comprising the step of deleting a tropoelastin derivative comprising the amino acid sequence of peptide 26A, or an amino acid sequence variant of peptide 26A from the biological polymer. Preferably the biological polymer is a protein.

The present invention also provides a tropoelastin derivative comprising the amino acid sequence of SHELgamma. SHELgamma has the amino acid sequence:

SAMGALVGLGVPGLGVGAGVPGFGAGADEGVRRSLSPELREGDPSSSQHLPSTPSSPR VPGALAAAKAAKYGAAVPGVLGGLGALGGVGIPGGWGAGPAAAAAAAKAAAKAAQFG LVGAAGLGGLGVGGLGVPGVGGLGGIPPAAAAKAAKYGAAGLGGVLGGAGQFPLGGVA ARPGFGLSPIFPGGACLGKACGRKRK (SEQ ID NO : 9) . The invention also provides an amino acid sequence variant of the derivative comprising the amino acid sequence of SHELgamma.

The invention also provides a polynucleotide encoding a tropoelastin derivative, the derivative comprising the amino acid sequence of SHELgamma. The nucleotide sequence of the polynucleotide SHELgamma (SEQ ID NO: 8) is shown in Figure 8. In this nucleotide sequence, the first 9 codons from nucleotide position 948 to 974 are derived from the glutathione S-transferase (GST) fusion nucleotide sequence. Preferably the polynucleotide comprises the nucleotide sequence shown in Figure 8. More preferably the polynucleotide comprises the nucleotide sequence shown in Figure 8 from nucleotide sequence position 975 to 1547. The invention also provides a polynucleotide encoding an amino acid sequence variant of the derivative comprising the amino acid sequence of SHELgamma.

The present invention also provides a polynucleotide encoding a tropoelastin derivative, the derivative comprising the amino acid sequence of SHELgamma excluding exon 26A. The nucleotide sequence of the polynucleotide SHELgamma excluding exon 26A (SEQ ID NO: 6) is shown in Figure 7. In this nucleotide sequence, the first 5 codons from nucleotide position 948 to 962 are derived from the

GST nucleotide sequence. SHELgamma excluding exon 26A has the following amino acid sequence:

VPGALAAAKAAKYGAAVPGVLGGLGALGGVGIPGGWGAGPAAAAAAAKAAAKAAQFG

LVGAAGLGGLGVGGLGVPGVGGLGGIPPAAAAKAAKYGAAGLGGVLGGAGQFPLGGVA ARPGFGLSPIFPGGACLGKACGRKRK (SEQ ID NO : 7) .

Preferably the polynucleotide comprises the nucleotide sequence shown in SEQ ID NO: 6. More preferably the polynucleotide comprises the nucleotide sequence shown in SEQ ID NO: 6 from nucleotide sequence position 15 to 441. The invention also provides a polynucleotide encoding an amino acid sequence variant of the derivative comprising the amino acid sequence of SHELgamma excluding exon 26A.

The invention also provides a tropoelastin derivative comprising the amino acid sequence of SHELgamma excluding exon 26A.

The invention also provides an amino acid sequence variant of the derivative comprising SHELgamma excluding exon 26A. The derivatives of the invention based on SHELgamma can also be produced by in vi tro biochemical cleavage of tropoelastin products such as SHEL, so as to release a carboxy- terminal fragment. The carboxy-ter inal fragment may be purified by reverse phase HPLC .

The present invention also provides a tropoelastin derivative comprising the amino acid sequence of SHEL31- 36. SHEL31-36 has the following amino acid sequence: GIPPAAAAKAAKYGAAGLGGVLGGAGQFPLGGVAARPGFGLSPIFPGGACLGKACG- RKRK (SEQ ID NO: 10) .

SHEL31-36 retains a crosslinking domain. As a consequence of its elastin-like properties, it is envisaged that this and related tropoelastin derivatives can be used to interfere with tropoelastin deposition and formation of unaltered elastic fibre.

The invention also provides an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL31-36. The invention also provides a polynucleotide encoding a tropoelastin derivative, the derivative comprising the amino acid sequence of SHEL31-36. Preferably the polynucleotide comprises the nucleotide sequence shown in Figure 1 (SEQ ID NO:l) from nucleotide position 2022 to 2210.

The invention also provides a polynucleotide encoding an amino acid variant of the derivative comprising the amino acid sequence of SHEL31-36.

The present invention also provides a tropoelastin derivative, comprising the amino acid sequence of SHEL32-

36. SHEL32-36 has the following amino acid sequence: GAAGLGGVLGGAGQFPLGGVAARPGFGLSPIFPGGACLGKACGRKRK (SEQ ID NO: 11) .

The invention also provides an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL32-36.

The invention also provides a polynucleotide encoding a tropoelastin derivative, the derivative comprising the amino acid sequence of SHEL32-36. Preferably the polynucleotide comprises the nucleotide sequence shown in Figure 1 (SEQ ID NO: 1) from nucleotide position 2061 to 2210.

The present invention also provides a polynucleotide encoding an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL32-36.

As a consequence of its elastin-like properties, it is envisaged that SHEL32-36 and related tropoelastin derivatives can be used to interfere with tropoelastin deposition and formation of an unaltered elastic fibre.

The present invention also provides a tropoelastin derivative, comprising the amino acid sequence of SHEL26- 36. SHEL26-36 has the following amino acid sequence: AAAGLGAGIPGLGVGVGVPGLGVGAGVPGLGVGAGVPGFGAGADEGVRRSLSPELREGD PSSSQHLPSTPSSPRVPGALAAAKAAKYGAAVPGVLGGLGALGGVGIPGGWGAGPAAA AAAAKAAAKAAQFGLVGAAGLGGLGVGGLGVPGVGGLGGIPPAAAAKAAKYGAAGLGGV LGGAGQFPLGGVAARPGFGLSPIFPGGACLGKACGRKRK (SEQ ID NO: 14)

The invention also provides an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL26-36.

The invention also provides a polynucleotide encoding a tropoelastin derivative, the derivative comprising the amino acid sequence of SHEL26-36. Preferably the polynucleotide comprises the nucleotide sequence shown in

Figure 1 from nucleotide position 1554-2210.

The present invention also provides a tropoelastin derivative, comprising the amino acid sequence of SHEL26- 36 excluding exon 26A. SHEL26-36 excluding exon 26A has the following amino acid sequence:

AAAGLGAGIPGLGVGVGVPGLGVGAGVPGLGVGAGVPGFGAVPGALAAAKAAKYGAAVP GVLGGLGALGGVGIPGGWGAGPAAAAAAAKAAAKAAQFGLVGAAGLGGLGVGGLGVPG VGGLGGIPPAAAAKAAKYGAAGLGGVLGGAGQFPLGGVAARPGFGLSPIFPGGACLGKA CGRKRK (SEQ ID NO : 15) The invention also provides an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL26-36 excluding exon 26A.

The invention also provides a polynucleotide encoding a tropoelastin derivative, the derivative comprising the amino acid sequence of SHEL26-36 excluding exon 26A.

Preferably the polynucleotide comprises the nucleotide sequence shown in Figure 1 from nucleotide position 1554 to 1676 contiguous with 1776 to 2210.

The present invention also provides a polynucleotide encoding an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL26-36. In another aspect the present invention provides a formulation comprising a tropoelastin derivative, a variant of the derivative or a hybrid molecule of the invention, together with a carrier or diluent.

Formulations of the derivatives, variants or hybrid molecules of the invention can be prepared in accordance with standard techniques appropriate to the field in which they are to be used.

The polynucleotides and synthetic polynucleotides of the invention can be provided in association with other polynucleotide sequences including 5' and 3' untranslated sequences, and 5' upstream and 3' downstream transcriptional regulatory sequences. The polynucleotides and synthetic polynucleotides may be provided as a recombinant DNA molecule including plasmid DNA. The polynucleotides and synthetic polynucleotides of the invention can be prepared using the techniques of chemical synthesis or recombinant DNA technology, or by a combination of both techniques .

In a further aspect the invention provides a vector comprising a polynucleotide or synthetic polynucleotide encoding a tropoelastin derivative, a variant of the derivative or a hybrid molecule of the invention.

Vectors useful in this invention include plasmids, phages and phagemids . The polynucleotides and synthetic polynucleotides of the present invention can also be used in integrative expression systems or lytic or comparable expression systems.

Suitable vectors will generally contain origins of replication and control sequences which are derived from species compatible with the intended expression host. Typically these vectors include a promoter located upstream from the polynucleotide, together with a ribosome binding site if intended for prokaryotic expression, and a phenotypic selection gene such as one conferring antibiotic resistance or supplying an auxotrophic requirement. For production vectors, vectors which provide for enhanced stability through partitioning may be chosen. Where integrative vectors are used it is not necessary for the vector to have an origin of replication. Lytic and other comparable expression systems do not need to have those functions required for maintenance of vectors in hosts. For E. coli typical vectors include pBR322, pBluescript II SK⁺, pGEX-2T, pTrc99A, pET series vectors, particularly pET3d, (Studier et al . , 1990) and derivatives of these vectors. Derivatives include those plasmids with a modified protease recognition sequence to facilitate purification of a protein domain.

In another aspect the invention provides a cell capable of expressing a polynucleotide or a synthetic polynucleotide which encodes a derivative or variant of the invention, or a polynucleotide which encodes a hybrid molecule of the invention.

A preferred expression system is an E. coli expression system. However, the invention includes within its scope the use of other hosts capable of expressing protein from the polynucleotides designed for use in E. coli . The invention also includes the use of polynucleotides and synthetic polynucleotides suitable for use in other expression systems such as other microbial expression systems. These other expression systems include yeast, and bacterial expression systems, insect cell expression systems, and expression systems involving other eukaryotic cell lines or whole organisms.

Examples of E. coli hosts include E. coli B strain derivatives (Studier et al , 1990), and K-strain derivatives such as NM522 (Gough and Murray, 1983) and XLl-Blue (Bullock et al , 1987) .

In a further aspect the present invention provides an expression product. In the specification and claims, "expression product" means a derivative or variant of the invention expressed by a cell containing a polynucleotide or a synthetic polynucleotide encoding a derivative or variant of the invention.

The expression products of the invention may be fused expression products which include all or part of a protein encoded by the vector in peptide linkage with the derivative or variant. They may also include, for example, an N-terminal methionine or other additional residues which do not permanently impair the elastin-like, or macro-molecular binding properties of the product.

Typically the fusion is to the N-terminus of the expression product. An example of a suitable protein is to the C-terminus of glutathione S-transferase . The fused protein sequence may be chosen in order to cause the expression product to be secreted or expressed as a cell surface protein to simplify purification or expressed as a cytoplasmic protein.

The expressed fusion products may subsequently be treated to remove the fused protein sequences to provide free tropoelastin derivative or variant. Treatment is typically through protease treatment or, in the case of secretion, removal is effected by endogenous host secretion machinery. An example of this is secretion by yeasts . Non-fused systems include the introduction of or use of a pre-existing methionine codon. An example of this is the use of pET3a or pET3d in E. coli .

In another aspect the invention provides a polynucleotide encoding an expression product of the invention.

In another aspect the present invention provides a formulation comprising an expression product of the invention together with a carrier or diluent. The formulation of the expression product can be prepared in accordance with standard techniques appropriate to the field in which they are to be used.

According to a further aspect of the present invention there is provided a method for producing a tropoelastin derivative or a variant of the derivative comprising providing a vector containing a polynucleotide or a synthetic polynucleotide encoding the derivative or variant; introducing the vector into a suitable host cell; maintaining the cell in conditions suitable for expression of the polynucleotide or synthetic polynucleotide and isolating the derivative or variant of the invention. The method can be applied to the production of the expression products and hybrid molecules (in which the hybrid molecules comprise the peptide 26A or a variant thereof and a further amino acid sequence) of the invention, by providing a vector containing a polynucleotide encoding an expression product or a hybrid molecule; introducing the vector into a suitable host cell; maintaining the cell in conditions suitable for expression of the polynucleotide and isolating the expression product or hybrid molecule. In one embodiment, the polynucleotide or synthetic polynucleotide encoding the derivative, variant, expression product or hybrid molecule of the invention is expressed in a host cell which is maintained in culture in vi tro .

Alternatively, the polynucleotide or synthetic polynucleotide encoding the derivative, variant, expression product or hybrid molecule of the invention is expressed in a host cell which is maintained in vivo . Thus, in another embodiment, the polynucleotide or synthetic polynucleotide encoding the derivative, variant, expression product or hybrid molecule of the invention is expressed in a transgenic animal . Methods for the generation of transgenic animals are known in the art.

Exemplary methods are described in Slack et al . 1991 and Janne et al . 1992.

The tropoelastin derivatives, variants of the derivatives, and hybrid molecules (in which the hybrid molecules comprise the peptide 26A or a variant thereof and a further amino acid sequence) of the invention may be produced by solid phase peptide synthesis, including, for example, the methods of synthesis disclosed in Merrifield (1963) or Knorr et al (1989) . Examples of peptide synthesis also include the synthesis methods used by peptide synthesisers of Perkin Elmer/Applied Biosystems, CA, US. As an alternative to cell synthesis from a polynucleotide or synthetic polynucleotide, the expression products of the invention may be produced by solid phase peptide synthesis.

In a further aspect the present invention provides an implant formed from at least one tropoelastin derivative and/or variant of the derivative of the invention. The implant may alternatively contain at least one expression product and/or at least one hybrid molecule of the invention.

The implants are formed into the required shape by cross-linking the tropoelastin derivative, variant of the derivative, expression product, or hybrid molecule of the invention, in a mould which conforms to the desired shape of the implant. Where the implant is required to be used in sheet form the tropoelastin derivative, variant of the derivative, expression product, or hybrid molecule of the invention can be cross-linked on a flat surface. Relevant methodologies are described in, for example, US Patent No. 4 474 851 and US Patent No. 5 250 516. The elastomeric materials may be exclusively prepared from one or more tropoelastin derivatives, variants of the derivative, expression products, or hybrid molecules of the invention or may be composites prepared from one or more of these constituents together with other materials.

The tropoelastin derivatives or variants of the derivatives can be cross-linked to form elastin or elastin-like material or can be cross-linked in conjunction with other biological or synthetic molecules to form a composite material.

Thus in another aspect the invention provides a cross-linked complex which comprises at least one tropoelastin derivative of the invention and/or at least one variant of a derivative of the invention. The crosslinked complexes may additionally contain at least one expression product and/or at least one hybrid molecule of the invention, which may be cross-linked to the at least one tropoelastin derivative and/or variant of the derivative of the invention. The cross-linking of the tropoelastin derivatives, variants of the derivatives, hybrid molecules and expression products of the invention can be achieved by chemical oxidation of lysine side chains using processes such as ruthenium tetroxide mediated oxidation and quinone mediated oxidation, or by using homobifunctional chemical cross-linking agents such as dithiobis

(succinimidylpropionate) , dimethyl adipimidate or dimethyl pimelimidate . Glutaraldehyde cross-linking is an important addition to this repetoire. Another alternative is the cross-linking of lysine and glutamic side chains. The tropoelastin derivatives, variants of the derivatives, hybrid molecules and expression products of the invention may also be enzymatically cross-linked by methods including lysyl oxidase mediated oxidation or may be cross-linked using gamma irradiation.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1: Nucleotide (SEQ ID NO: 1) and predicted amino acid (SEQ ID NO: 2) sequences of synthetic human tropoelastin SHEL . The upper (numbered) nucleotide sequence represents the coding strand.

Figure 2: Alignment of SHEL (SEQ ID NO : 2 ) (upper line) and SHELδ26A (SEQ ID NO : 3) amino acid sequences.

Figure 3: Nucleotide (SEQ ID NO: 4) and predicted amino acid (SEQ ID NO: 5) sequences of SHELδmodified.

Figure 4: Alignment of SHELδmodified (SEQ ID NO: 4)

(upper line) and SHEL (SEQ ID NO:l) nucleotide sequences.

Figure 5: Alignment of SHELδmodified (SEQ ID NO:

5) (lower line) and SHEL (SEQ ID NO : 1) amino acid sequences.

Figure 6A: HPLC elution profile of GST-exon 26A fusion protein tropoelastin derivative loaded in from heparin sepharose. 6B : Binding of peptide 26A (SEQ ID NO: 12 and SEQ ID NO: 13) to glycosammoglycans .

Figure 7: Nucleotide (SEQ ID NO: 6) and predicted amino acid (SEQ ID NO: 7) sequences of SHELgamma excluding exon 26A.

Figure 8: Nucleotide (SEQ ID NO: 8) and predicted amino acid (SEQ ID NO : 9) sequences of SHELgamma.

BEST METHOD OF PERFORMING THE INVENTION The recombinant and synthetic procedures used for the synthesis of the derivatives, variants, expression products and hybrid molecules of the invention are described in standard texts such as Sambrook et al (1989) . Tropoelastin nucleotide sequences may be modified so as to provide derivatives, variants, expression products or hybrid molecules, by conventional site-directed or random mutagenesis. The sequences may also be modified by oligonucleotide-directed mutagenesis, which comprises the following steps: 1. synthesis of an oligonucleotide with a sequence that contains the desired nucleotide substitution (mutation) ;

2. hybridising the oligonucleotide to a template comprising a structural sequence encoding tropoelastin; and

3. using a DNA polymerase to extend the oligonucleotide as a primer.

Another approach which is particularly suited to situations where a synthetic polynucleotide encoding the tropoelastin derivative is prepared from oligonucleotide blocks bounded by restriction sites, is cassette mutagenesis where entire restriction fragments are replaced.

Purification of the derivatives, variants, expression products or hybrid molecules of the invention is performed using standard techniques including HPLC. The actual sequence of steps in the purification of a particular derivative, variant, expression product or hybrid molecule is limited by the environment from which the molecule is to be purified. By way of example, reference is made to the purification scheme disclosed in W094/14958.

Formulations in accordance with the invention are formulated in accordance with standard techniques .

The amount of derivative, variant, expression product or hybrid molecule that may be combined with a carrier or diluent to produce a single dosage will vary depending on the situation in which the formulation is to be used and the particular mode of administration.

It will be understood also that specific doses for any particular host may be influenced by factors such as the age, sex, weight and general health of the host as well as the particular characteristics of the derivative, variant, expression product or hybrid molecule of the invention being used, and how it is administered.

Injectable preparations, for example, sterile injectable aqueous or oleagenous suspensions may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally acceptable diluent or solvent. Among the acceptable vehicles or solvents that may be employed are water, Ringer's solution, alcohols and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose any bland fixed oil may be employed including synthetic mono- or diglycerides . In addition, fatty acids such as oleic acid and organic solvents find use in the preparation of injectables.

Routes of administration, dosages to be administered as well as frequency of administration are all factors which can be optimised using ordinary skill in the art. In addition, the derivatives, variants, expression products and hybrid molecules of the invention may be prepared as topical preparations for instance as anti- wrinkle and hand lotions using standard techniques for the preparation of such formulations . They may be prepared in aerosol form for, for instance, administration to a patient's lungs, or in the form of surgical implants, foods or industrial products by standard techniques.

SHEL

The preparation of SHEL is described in W094/14958. It is directly expressed as a full length human protein with a calculated molecular weight of 64kDa. The full nucleotide sequence and corresponding amino acid sequence of SHEL is shown in Figure 1. The preparation of pSHELF is described in W094/14958. pSHELF differs from the natural coding sequence (s) in a number of ways. As described in W094/14958, the untranslated regions present in the tropoelastin cDNA sequence were disregarded in designing the synthetic gene, and the nucleotides encoding the signal peptide were removed. Restriction endonuclease recognition sites were incorporated at regular intervals into the gene by typically altering only the third base of the relevant codons, thereby maintaining the primary sequence of the gene product. The facility for silent alteration of the coding sequence was also exploited to change the codon bias of the tropoelastin gene to that commonly found in highly expressed E. coli genes. [Genetics Computer Group (GCG) package version 7-UNIX using Codon Frequency and Gen Run Data: ecohigh-cod] . Two additional stop codons were added to the 3 ' -end, and an ATG start codon comprising a novel Ncol site was appended to the 5 '-end. Bam HI cloning sites were engineered at both ends of the synthetic sequence.

Since the gene contains no internal methionine residues, treatment of the newly-synthesized gene product (expressed directly or as a fusion with another gene) with cyanogen bromide would liberate a protein with the same or similar sequence as one form of natural tropoelastin comprising 731 amino acids. Other forms of processing are envisaged, which may generate tropoelastin species of the same or different lengths. Two stop codons were added in order to allow the possible use of the construct in suppressor hosts, and also to avoid any potential depletion of termination (release) factors for translation. As described in the following examples, the derivatives, pSHELFδ26A, pSHELFδ modified, pSHELgamma,

PSHEL31-36, pSHEL32-36 and pSHELgammaδ26A were derived from the pSHELF nucleotide sequence. These particular derivatives, and indeed the derivaties, variants, expression products and hybrid molecules of the invention can equally be derived from a native human or non -human tropoelastin nucleotide sequence.

Example 1 : Construction of pSHELFδ26A and pSHELFδ modified

Mutagenesis was used with pSHELF to remove DNA corresponding to exon 26A. The sequence of the mutagenic primer was :

5 ' CGG GTT TCG GTG CTG TTC CGG GCG CGC TGG 3 ' This flanked either side of exon 26A by 15bp resulting in its precise deletion. A second selection primer, which mutates a unique restriction site to another restriction site is normally used in the protocol but was not in this case since deletion of exon 26A also resulted in the deletion of a unique restriction site, Pmll . The enzyme Pmll was used to treat the mutation reaction to linearise any unπvutated parental plasmid and consequently to enrich for mutant plasmid. The reaction mixture was used to transform competent BMH17-18 mutS E. coli , defective in mismatch repair, by electroporation and the entire transformed culture was grown overnight in LB+ampicillin. Mixed plasmid DNA, containing both mutated and parental plasmids, was isolated from the culture and the plasmid DNA was digested with Pmll to linearise the parental plasmid. The plasmid DNA, now enriched for mutated plasmid, was used to transform E. coli HMS174 by electroporation and transformants selected on LB plates containing 75μgml^~1 ampicillin.

Colonies were grown overnight and plasmid mini- preparations performed. Constructs were screened using Pmll and those which were insensitive to digestion were further screened by Kpnl / PstI double digestion. Candidate clones were sequenced to verify the sequence, named pSHELFδmodified.

Sequencing confirmed the region immediately surrounding the deletion was correct. PstI and BssHII restriction sites surrounding the correct region of pSHELFδmodified were used to remove the desired segment and re-insert it into the corresponding site of pSHELF.

6.5μg pSHELF and 7.5μg pSHELFδmodified were digested with

BssHII, precipitated and digested with PstI. The appropriate three fragments were gel-purified and ligated. DNA was transformed into E. coli XLl-Blue and transformants selected on plates containing 75μgml^"1 ampicillin.

Plasmids were isolated by mini-preparations and screened using Bgll digestion. A candidate clone was further analysed by restriction enzyme digestion and sequenced, and named pSHELFδ26A.

Example 2 : Synthesis of Exon 26A The region of SHEL corresponding to exon 26A was amplified by PCR, with primers modified to introduce an in-frame BamHI site upstream and a stop codon downstream at the 3' end. Two forms were generated: one terminating in valine (26AV) and the other terminating in phenyalanine (26AF) . These forms are as follows:

GADEGVRRSLSPELREGDPSSSQHLPSTPSSPRV with properties: Molecular weight = 3588.80 Residues = 34

Average Residue Weight = 105.553 Charge = -1

Isoelectric point = 5.71 and GADEGVRRSLSPELREGDPSSSQHLPSTPSSPRF

A 26A coding region was expressed as a glutathione S- transferase (GST) fusion protein.

Example 3 : Glvcosaminoglycan binding activity of Exon 26A Ultrafiltration assay methodology was developed to examine and quantify interactions occurring in vi tro between the 26A region and purified extracellular matrix glcosaminoglycans . GST26A fusion protein and tropoelastin were compared with GST and tropoelastin lacking exon 26A at physiologicaly relevant conditions of pH and ionic strength. Experimental evidence supports the notion that peptide 26A (26AF and 26AV) binds GAGs. Immobilised heparin column binding shows that GST26A binds more tightly than does GST, and requires a higher sodium chloride concentration for elution (Figure 6B) . Furthermore, GST26A fusion protein binds radioactive heparin with greater efficiencies than GST, and these can be compared with GAGs including chondroitin sulphates and keratin sulphates . An implication of this is that GAGs binding to tropoelastin can be adjusted based upon the content of 26A. Cross-linked tropoelastin would be expected to show differential binding to GAGs based on the relative amounts of SHEL vs. SHELδ26A.

In summary, these studies reveal that the 26A region is a functional glvcosaminoglycan binding domain, which functions in intact tropoelastin. It is also active when isolated as a fusion entity yet displays no detectable structure in the absence of bound GAG. Furthermore, panel competition studies indicate a preference for those GAGs found in close association with the elastic fibre in the extracellular matrix. Example 4 : Construction of pSHELqamma, PSHEL31-36, PSHEL32-36 and pSHELαammaδ26A pSHELgamma is derived from the pSHELgamma construct disclosed in W094/14958. pSHEL31-36, pSHEL32-36 and pSHELgammaδ26A were derived from pSHELgamma. pSHELgamma was modified by introducing an oligonucleotide linker at the Kpnl site. This encoded a factor Xa cleavage site which could be utilised in subsequent constructs. PCR and site directed mutagenesis was then used to generate further, shorter forms which provided fusions with GST. Constructs were DNA sequenced for verification. Induced protein was isolated as GST-fusion proteins, which were subsequently bound to glutathione agarose. Protease cleavage was optional where fusion proteins were desired; otherwise the cleaved proteins and peptides were further purified by reverse phase HPLC.

INDUSTRIAL APPLICATION The derivatives and expression products of the invention are of use in inter alia the medical, pharmaceutical, veterinary and cosmetic fields.

REFERENCES

1. Indik Z, Yeh H, Ornstein-Goldstein N, Sheppard P, Anderson N, Rosenbloom JC, Peltonen L and Rosenbloom J (1987) PNAS (USA) 84 5680-5684

2. Indik Z, Abrams W.R., Kucich U, Gibson C.W., Mecham R.P. and Rosenbloom J (1990) Arch. Biochem Biophys 280 80-86

3. Oliver L, Luvalle PA, Davidson J.M., Rosenbloom J, Mathew C.G., Betser A.J. and Boyd CD. (1987) Collagen Rel Res 7 77-89

4. Sambrook J., Fritsch E.F., and Maniatis T. (1989) Molecular cloning: a laboratory manual, second edition Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York

5. Bressan G.M., Argos P. and Stanley K.K. (1987) Biochemistry 26 1497-11503

6. Raju K. aand Anwar R.A. (1987) J. Biol Chem 262 5755- 5762

7. Pierce R.A. , Alatawi A, Deak S.B. & Boyd CD. (1992; Genomics 12 651-658

8. Lipman and Pearson (1985) Science 227,1435.

9. Bedell-Hogan, D. , Trackman, P., Abrams, W. , Rosenbloom, J. and Kagan H. (1993) J. Biol. Chem. 268, 10345-10350

10. Studier, F. W. , Rosenberg, A. H., Dunn, J. J. and

Dubendorff, J. W. (1990) Methods Enzy ol . 185, 60-89

11. Gough, J., and Murray, N. (1983) J. Mol . Biol. 166, 1 - 19

12. Bullock, W. O., Fernandez, J. M. and Short, J. M.

(1987) BioTechniques 5, 376-379

13. Slack, J. L., Liska, D. J. and Bornstein P. (1991) Mol. Cell Biol. 11: 2066-2074

14. Janne, J., Hyttinen, J. M. , Peura, T., Tolvanen, M. , Alhonen, L. And Halmekyto M. (1992) Ann. Med. 24:

273-280.

15. Merrifield, R.B., (1963) J. Am. Chem. Soc . 85: 2149-2154.

16. Knorr R. , Trzeciak, Bannarth W. , Gillessen, D. (1989) Tetrahedron Letters 30: 1927-1930

SEQUENCE LISTING

(1) GENERAL INFORMATION:

(i) APPLICANT: WEISS, ANTHONY S

UNIVERSITY, SYDNEY

(ii) TITLE OF INVENTION: TROPOELASTIN DERIVATIVES

(iii) NUMBER OF SEQUENCES: 15

(iv) CORRESPONDENCE ADDRESS:

(A) ADDRESSEE: GRIFFITH HACK

(B) STREET: 168 WALKER STREET

(C) CITY: NORTH SYDNEY

(D) STATE: NEW SOUTH WALES

(E) COUNTRY: AUSTRALIA

(F) ZIP: 2060

(v) COMPUTER READABLE FORM:

(A) MEDIUM TYPE: Floppy disk

(B) COMPUTER: IBM PC compatible

(C) OPERATING SYSTEM: PC-DOS/MS-DOS

(D) SOFTWARE: Patentin Release #1.0, Version #1.30

(vi) CURRENT APPLICATION DATA:

(A) APPLICATION NUMBER: AU

(B) FILING DATE:

(C) CLASSIFICATION:

(vii) PRIOR APPLICATION DATA:

(A) APPLICATION NUMBER: AU P08117

(B) FILING DATE: 18-JUL-1997

(viii) ATTORNEY/AGENT INFORMATION: (A) NAME: GUMLEY, THOMAS P

(C) REFERENCE/DOCKET NUMBER: 04828ZK

(ix) TELECOMMUNICATION INFORMATION:

(A) TELEPHONE: 61 2 9957 5944

(B) TELEFAX: 61 2 9957 6288

(C) TELEX: 26547

(2) INFORMATION FOR SEQ ID NO:l: (i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 2210 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: double

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: DNA (genomic)

(iii) HYPOTHETICAL: YES

(iv) ANTI-SENSE: NO

(xi) SEQUENCE DESCRIPTION: SEQ ID NO : 1 :

GATCCATGGG TGGCGTTCCG GGTGCTATCC CGGGTGGCGT TCCGGGTGGT GTATTCTACC 60

CAGGCGCGGG TCTGGGTGCA CTGGGCGGTG GTGCGCTGGG CCCGGGTGGT AAACCGCTGA 120

AACCGGTTCC AGGCGGTCTG GCAGGTGCTG GTCTGGGTGC AGGTCTGGGC GCGTTCCCGG 180

CGGTTACCTT CCCGGGTGCT CTGGTTCCGG GTGGCGTTGC AGACGCAGCT GCTGCGTACA 240

AAGCGGCAAA GGCAGGTGCG GGTCTGGGCG GGGTACCAGG TGTTGGCGGT CTGGGTGTAT 300

CTGCTGGCGC AGTTGTTCCG CAGCCGGGTG CAGGTGTAAA ACCGGGCAAA GTTCCAGGTG 360

TTGGTCTGCC GGGCGTATAC CCGGGTGGTG TTCTGCCGGG CGCGCGTTTC CCAGGTGTTG 420

GTGTACTGCC GGGCGTTCCG ACCGGTGCAG GTGTTAAACC GAAGGCACCA GGTGTAGGCG 480

GCGCGTTCGC GGGTATCCCG GGTGTTGGCC CGTTCGGTGG TCCGCAGCCA GGCGTTCCGC 540

TGGGTTACCC GATCAAAGCG CCGAAGCTTC CAGGTGGCTA CGGTCTGCCG TACACCACCG 600

GTAAACTGCC GTACGGCTAC GGTCCGGGTG GCGTAGCAGG TGCTGCGGGT AAAGCAGGCT 660

ACCCAACCGG TACTGGTGTT GGTCCGCAGG CTGCTGCGGC AGCTGCGGCG AAGGCAGCAG 720

CAAAATTCGG CGCGGGTGCA GCGGGTGTTC TGCCGGGCGT AGGTGGTGCT GGCGTTCCGG 780

GTGTTCCAGG TGCGATCCCG GGCATCGGTG GTATCGCAGG CGTAGGTACT CCGGCGGCCG 840 CTGCGGCTGC GGCAGCTGCG GCGAAAGCAG CTAAATACGG TGCGGCAGCA GGCCTGGTTC 900

CGGGTGGTCC AGGCTTCGGT CCGGGTGTTG TAGGCGTTCC GGGTGCTGGT GTTCCGGGCG 960

TAGGTGTTCC AGGTGCGGGC ATCCCGGTTG TACCGGGTGC AGGTATCCCG GGCGCTGCGG 1020

TTCCAGGTGT TGTATCCCCG GAAGCGGCAG CTAAGGCTGC TGCGAAAGCT GCGAAATACG 1080

GAGCTCGTCC GGGCGTTGGT GTTGGTGGCA TCCCGACCTA CGGTGTAGGT GCAGGCGGTT 1140

TCCCAGGTTT CGGCGTTGGT GTTGGTGGCA TCCCGGGTGT AGCTGGTGTT CCGTCTGTTG 1200

GTGGCGTACC GGGTGTTGGT GGCGTTCCAG GTGTAGGTAT CTCCCCGGAA GCGCAGGCAG 1260

CTGCGGCAGC TAAAGCAGCG AAGTACGGCG TTGGTACTCC GGCGGCAGCA GCTGCTAAAG 1320

CAGCGGCTAA AGCAGCGCAG TTCGGACTAG TTCCGGGCGT AGGTGTTGCG CCAGGTGTTG 1380

GCGTAGCACC GGGTGTTGGT GTTGCTCCGG GCGTAGGTCT GGCACCGGGT GTTGGCGTTG 1440

CACCAGGTGT AGGTGTTGCG CCGGGCGTTG GTGTAGCACC GGGTATCGGT CCGGGTGGCG 1500

TTGCGGCTGC TGCGAAATCT GCTGCGAAGG TTGCTGCGAA AGCGCAGCTG CGTGCAGCAG 1560

CTGGTCTGGG TGCGGGCATC CCAGGTCTGG GTGTAGGTGT TGGTGTTCCG GGCCTGGGTG 1620

TAGGTGCAGG GGTACCGGGC CTGGGTGTTG GTGCAGGCGT TCCGGGTTTC GGTGCTGGCG 1680

CGGACGAAGG TGTACGTCGT TCCCTGTCTC CAGAACTGCG TGAAGGTGAC CCGTCCTCTT 1740

CCCAGCACCT GCCGTCTACC CCGTCCTCTC CACGTGTTCC GGGCGCGCTG GCTGCTGCGA 1800

AAGCGGCGAA ATACGGTGCA GCGGTTCCGG GTGTACTGGG CGGTCTGGGT GCTCTGGGCG 1860

GTGTTGGTAT CCCGGGCGGT GTTGTAGGTG CAGGCCCAGC TGCAGCTGCT GCTGCGGCAA 1920

AGGCAGCGGC GAAAGCAGCT CAGTTCGGTC TGGTTGGTGC AGCAGGTCTG GGCGGTCTGG 1980

GTGTTGGCGG TCTGGGTGTA CCGGGCGTTG GTGGTCTGGG TGGCATCCCG CCGGCGGCGG 2040

CAGCTAAAGC GGCTAAATAC GGTGCAGCAG GTCTGGGTGG CGTTCTGGGT GGTGCTGGTC 2100

AGTTCCCACT GGGCGGTGTA GCGGCACGTC CGGGTTTCGG TCTGTCCCCG ATCTTCCCAG 2160

GCGGTGCATG CCTGGGTAAA GCTTGCGGCC GTAAACGTAA ATAATGATAG 2210 (2) INFORMATION FOR SEQ ID NO : 2 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 733 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2:

Ser Met Gly Gly Val Pro Gly Ala lie Pro Gly Gly Val Pro Gly Gly

1 5 10 15

Val Phe Tyr Pro Gly Ala Gly Leu Gly Ala Leu Gly Gly Gly Ala Leu 20 25 30

Gly Pro Gly Gly Lys Pro Leu Lys Pro Val Pro Gly Gly Leu Ala Gly 35 40 45

Ala Gly Leu Gly Ala Gly Leu Gly Ala Phe Pro Ala Val Thr Phe Pro 50 55 60

Gly Ala Leu Val Pro Gly Gly Val Ala Asp Ala Ala Ala Ala Tyr Lys 65 70 75 80

Ala Ala Lys Ala Gly Ala Gly Leu Gly Gly Val Pro Gly Val Gly Gly 85 90 95

Leu Gly Val Ser Ala Gly Ala Val Val Pro Gin Pro Gly Ala Gly Val 100 105 110

Lys Pro Gly Lys Val Pro Gly Val Gly Leu Pro Gly Val Tyr Pro Gly 115 120 125

Gly Val Leu Pro Gly Ala Arg Phe Pro Gly Val Gly Val Leu Pro Gly 130 135 140

Val Pro Thr Gly Ala Gly Val Lys Pro Lys Ala Pro Gly Val Gly Gly 145 150 155 160

Ala Phe Ala Gly lie Pro Gly Val Gly Pro Phe Gly Gly Pro Gin Pro 165 170 175

Gly Val Pro Leu Gly Tyr Pro lie Lys Ala Pro Lys Leu Pro Gly Gly 180 185 190

Tyr Gly Leu Pro Tyr Thr Thr Gly Lys Leu Pro Tyr Gly Tyr Gly Pro 195 200 205

Gly Gly Val Ala Gly Ala Ala Gly Lys Ala Gly Tyr Pro Thr Gly Thr 210 215 220

Gly Val Gly Pro Gin Ala Ala Ala Ala Ala Ala Ala Lys Ala Ala Ala 225 230 235 240

Lys Phe Gly Ala Gly Ala Ala Gly Val Leu Pro Gly Val Gly Gly Ala 245 250 255

Gly Val Pro Gly Val Pro Gly Ala He Pro Gly He Gly Gly He Ala 260 265 270

Gly Val Gly Thr Pro Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Lys 275 280 285

Ala Ala Lys Tyr Gly Ala Ala Ala Gly Leu Val Pro Gly Gly Pro Gly 290 295 300

Phe Gly Pro Gly Val Val Gly Val Pro Gly Ala Gly Val Pro Gly Val 305 310 315 320

Gly Val Pro Gly Ala Gly He Pro Val Val Pro Gly Ala Gly He Pro 325 330 335

Gly Ala Ala Val Pro Gly Val Val Ser Pro Glu Ala Ala Ala Lys Ala 340 345 350

Ala Ala Lys Ala Ala Lys Tyr Gly Ala Arg Pro Gly Val Gly Val Gly 355 360 365

Gly He Pro Thr Tyr Gly Val Gly Ala Gly Gly Phe Pro Gly Phe Gly 370 375 380

Val Gly Val Gly Gly He Pro Gly Val Ala Gly Val Pro Ser Val Gly 385 390 395 400

Gly Val Pro Gly Val Gly Gly Val Pro Gly Val Gly He Ser Pro Glu 405 410 415 Ala Gin Ala Ala Ala Ala Ala Lys Ala Ala Lys Tyr Gly Val Gly Thr 420 425 430

Pro Ala Ala Ala Ala Ala Lys Ala Ala Ala Lys Ala Ala Gin Phe Gly 435 440 445

Leu Val Pro Gly Val Gly Val Ala Pro Gly Val Gly Val Ala Pro Gly 450 455 460

Val Gly Val Ala Pro Gly Val Gly Leu Ala Pro Gly Val Gly Val Ala 465 470 475 480

Pro Gly Val Gly Val Ala Pro Gly Val Gly Val Ala Pro Gly He Gly 485 490 495

Pro Gly Gly Val Ala Ala Ala Ala Lys Ser Ala Ala Lys Val Ala Ala 500 505 510

Lys Ala Gin Leu Arg Ala Ala Ala Gly Leu Gly Ala Gly He Pro Gly 515 520 525

Leu Gly Val Gly Val Gly Val Pro Gly Leu Gly Val Gly Ala Gly Val 530 535 540

Pro Gly Leu Gly Val Gly Ala Gly Val Pro Gly Phe Gly Ala Gly Ala 545 550 555 560

Asp Glu Gly Val Arg Arg Ser Leu Ser Pro Glu Leu Arg Glu Gly Asp 565 570 575

Pro Ser Ser Ser Gin His Leu Pro Ser Thr Pro Ser Ser Pro Arg Val 580 585 590

Pro Gly Ala Leu Ala Ala Ala Lys Ala Ala Lys Tyr Gly Ala Ala Val 595 600 605

Pro Gly Val Leu Gly Gly Leu Gly Ala Leu Gly Gly Val Gly He Pro 610 615 620

Gly Gly Val Val Gly Ala Gly Pro Ala Ala Ala Ala Ala Ala Ala Lys 625 630 635 640

Ala Ala Ala Lys Ala Ala Gin Phe Gly Leu Val Gly Ala Ala Gly Leu 645 650 655 Gly Gly Leu Gly Val Gly Gly Leu Gly Val Pro Gly Val Gly Gly Leu 660 665 670

Gly Gly He Pro Pro Ala Ala Ala Ala Lys Ala Ala Lys Tyr Gly Ala 675 680 685

Ala Gly Leu Gly Gly Val Leu Gly Gly Ala Gly Gin Phe Pro Leu Gly 690 695 700

Gly Val Ala Ala Arg Pro Gly Phe Gly Leu Ser Pro He Phe Pro Gly 705 710 715 720

Gly Ala Cys Leu Gly Lys Ala Cys Gly Arg Lys Arg Lys 725 730

(2) INFORMATION FOR SEQ ID NO : 3 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 698 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO : 3 :

Gly Gly Val Pro Gly Ala He Pro Gly Gly Val Pro Gly Gly Val Phe 1 5 10 15

Tyr Pro Gly Ala Gly Leu Gly Ala Leu Gly Gly Gly Ala Leu Gly Pro 20 25 30

Gly Gly Lys Pro Leu Lys Pro Val Pro Gly Gly Leu Ala Gly Ala Gly 35 40 45

Leu Gly Ala Gly Leu Gly Ala Phe Pro Ala Val Thr Phe Pro Gly Ala 50 55 60

Leu Val Pro Gly Gly Val Ala Asp Ala Ala Ala Ala Tyr Lys Ala Ala 65 70 75 80

Lys Ala Gly Ala Gly Leu Gly Gly Val Pro Gly Val Gly Gly Leu Gly 85 90 95

Val Ser Ala Gly Ala Val Val Pro Gin Pro Gly Ala Gly Val Lys Pro 100 105 110

Gly Lys Val Pro Gly Val Gly Leu Pro Gly Val Tyr Pro Gly Gly Val 115 120 125

Leu Pro Gly Ala Arg Phe Pro Gly Val Gly Val Leu Pro Gly Val Pro 130 135 140

Thr Gly Ala Gly Val Lys Pro Lys Ala Pro Gly Val Gly Gly Ala Phe 145 150 155 160

Ala Gly He Pro Gly Val Gly Pro Phe Gly Gly Pro Gin Pro Gly Val 165 170 175

Pro Leu Gly Tyr Pro He Lys Ala Pro Lys Leu Pro Gly Gly Tyr Gly 180 185 190

Leu Pro Tyr Thr Thr Gly Lys Leu Pro Tyr Gly Tyr Gly Pro Gly Gly 195 200 205

Val Ala Gly Ala Ala Gly Lys Ala Gly Tyr Pro Thr Gly Thr Gly Val 210 215 220

Gly Pro Gin Ala Ala Ala Ala Ala Ala Ala Lys Ala Ala Ala Lys Phe 225 230 235 240

Gly Ala Gly Ala Ala Gly Val Leu Pro Gly Val Gly Gly Ala Gly Val 245 250 255

Pro Gly Val Pro Gly Ala He Pro Gly He Gly Gly He Ala Gly Val 260 265 270

Gly Thr Pro Ala Ala Ala Ala Ala Ala Ala Ala Ala Ala Lys Ala Ala 275 280 285

Lys Tyr Gly Ala Ala Ala Gly Leu Val Pro Gly Gly Pro Gly Phe Gly 290 295 300

Pro Gly Val Val Gly Val Pro Gly Ala Gly Val Pro Gly Val Gly Val 305 310 315 320

Pro Gly Ala Gly He Pro Val Val Pro Gly Ala Gly He Pro Gly Ala 325 330 335 Ala Val Pro Gly Val Val Ser Pro Glu Ala Ala Ala Lys Ala Ala Ala 340 345 350

Lys Ala Ala Lys Tyr Gly Ala Arg Pro Gly Val Gly Val Gly Gly He 355 360 365

Pro Thr Tyr Gly Val Gly Ala Gly Gly Phe Pro Gly Phe Gly Val Gly 370 375 380

Val Gly Gly He Pro Gly Val Ala Gly Val Pro Ser Val Gly Gly Val 385 390 395 400

Pro Gly Val Gly Gly Val Pro Gly Val Gly He Ser Pro Glu Ala Gin 405 410 415

Ala Ala Ala Ala Ala Lys Ala Ala Lys Tyr Gly Val Gly Thr Pro Ala 420 425 430

Ala Ala Ala Ala Lys Ala Ala Ala Lys Ala Ala Gin Phe Gly Leu Val 435 440 445

Pro Gly Val Gly Val Ala Pro Gly Val Gly Val Ala Pro Gly Val Gly 450 455 460

Val Ala Pro Gly Val Gly Leu Ala Pro Gly Val Gly Val Ala Pro Gly 465 470 475 480

Val Gly Val Ala Pro Gly Val Gly Val Ala Pro Gly He Gly Pro Gly 485 490 495

Gly Val Ala Ala Ala Ala Lys Ser Ala Ala Lys Val Ala Ala Lys Ala 500 505 510

Gin Leu Arg Ala Ala Ala Gly Leu Gly Ala Gly He Pro Gly Leu Gly 515 520 525

Val Gly Val Gly Val Pro Gly Leu Gly Val Gly Ala Gly Val Pro Gly 530 535 540

Leu Gly Val Gly Ala Gly Val Pro Gly Phe Gly Ala Val Pro Gly Ala 545 550 555 560

Leu Ala Ala Ala Lys Ala Ala Lys Tyr Gly Ala Ala Val Pro Gly Val 565 570 575 Leu Gly Gly Leu Gly Ala Leu Gly Gly Val Gly He Pro Gly Gly Val 580 585 590

Val Gly Ala Gly Pro Ala Ala Ala Ala Ala Ala Ala Lys Ala Ala Ala 595 600 605

Lys Ala Ala Gin Phe Gly Leu Val Gly Ala Ala Gly Leu Gly Gly Leu 610 615 620

Gly Val Gly Gly Leu Gly Val Pro Gly Val Gly Gly Leu Gly Gly He 625 630 635 640

Pro Pro Ala Ala Ala Ala Lys Ala Ala Lys Tyr Gly Ala Ala Gly Leu 645 650 655

Gly Gly Val Leu Gly Gly Ala Gly Gin Phe Pro Leu Gly Gly Val Ala 660 665 670

Ala Arg Pro Gly Phe Gly Leu Ser Pro He Phe Pro Gly Gly Ala Cys 675 680 685

Leu Gly Lys Ala Cys Gly Arg Lys Arg Lys 690 695

(2) INFORMATION FOR SEQ ID NO: 4:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 1983 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: double

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: DNA (genomic)

(iii) HYPOTHETICAL: YES

(iv) ANTI-SENSE: NO

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 4:

ATGGGTGGCG TTCCGGGTGC TGTTCCGGGT GGCGTTCCGG GTGGTGTATT CTACCCAGGC 60

GCGGGTTTCG GTGCTGTTCC GGGTGGCGTT GCAGACGCAG CTGCTGCGTA CAAAGCGGCA 120 AAGGCAGGTG CGGGTCTGGG CGGGGTACCA GGTGTTGGCG GTCTGGGTGT ATCTGCTGGC 180

GCAGTTGTTC CGCAGCCGGG TGCAGGTGTA AAACCGGGCA AAGTTCCAGG TGTTGGTCTG 240

CCGGGCGTAT ACCCGGGTTT CGGTGCTGTT CCGGGCGCGC GTTTCCCAGG TGTTGGTGTA 300

CTGCCGGGCG TTCCGACCGG TGCAGGTGTT AAACCGAAGG CACCAGGTGT AGGCGGCGCG 360

TTCGCGGGTA TCCCGGGTGT TGGCCCGTTC GGTGGTCCGC AGCCAGGCGT TCCGCTGGGT 420

TACCCGATCA AAGCGCCGAA GCTTCCAGGT GGCTACGGTC TGCCGTACAC CACCGGTAAA 480

CTGCCGTACG GCTACGGTCC GGGTGGCGTA GCAGGTGCTG CGGGTAAAGC AGGCTACCCA 540

ACCGGTACTG GTGTTGGTCC GCAGGCTGCT GCGGCAGCTG CGGCGAAGGC AGCAGCAAAA 600

TTCGGCGCGG GTGCAGCGGG TTTCGGTGCT GTTCCGGGCG TAGGTGGTGC TGGCGTTCCG 660

GGTGTTCCAG GTGCGATCCC GGGCATCGGT GGTATCGCAG GCGTAGGTAC TCCGGCGGCC 720

GCTGCGGCTG CGGCAGCTGC GGCGAAAGCA GCTAAATACG GTGCGGCAGC AGGCCTGGTT 780

CCGGGTGGTC CAGGCTTCGG TCCGGGTGTT GTAGGCGTTC CGGGTTTCGG TGCTGTTCCG 840

GGCGTAGGTG TTCCAGGTGC GGGCATCCCG GTTGTACCGG GTGCAGGTAT CCCGGGCGCT 900

GCGGGTTTCG GTGCTGTATC CCCGGAAGCG GCAGCTAAGG CTGCTGCGAA AGCTGCGAAA 960

TACGGAGCTC GTCCGGGCGT TGGTGTTGGT GGCATCCCGA CCTACGGTGT AGGTGCAGGC 1020

GGTTTCCCAG GTTTCGGCGT TGGTGTTGGT GGCATCCCGG GTGTAGCTGG TGTTCCGTCT 1080

GTTGGTGGCG TACCGGGTGT TGGTGGCGTT CCAGGTGTAG GTATCTCCCC GGAAGCGCAG 1140

GCAGCTGCGG CAGCTAAAGC AGCGAAGTAC GGCGTTGGTA CTCCGGCGGC AGCAGCTGCT 1200

AAAGCAGCGG CTAAAGCAGC GCAGTTCGGA CTAGTTCCGG GCGTAGGTGT TGCGCCAGGT 1260

GTTGGCGTAG CACCGGGTGT TGGTGTTGCT CCGGGCGTAG GTCTGGCACC GGGTGTTGGC 1320

GTTGCACCAG GTGTAGGTGT TGCGCCGGGC GTTGGTGTAG CACCGGGTAT CGGTCCGGGT 1380

GGCGTTGCGG CTGCTGCGAA ATCTGCTGCG AAGGTTGCTG CGAAAGCGCA GCTGCGTGCA 1440

GCAGCTGGTC TGGGTGCGGG CATCCCAGGT CTGGGTGTAG GTGTTGGTGT TCCGGGCCTG 1500 GGTGTAGGTG CAGGGGTACC GGGCCTGGGT GTTGGTGCAG GCGTTCCGGG TTTCGGTGCT 1560

GTTCCGGGCG CGCTGGCTGC TGCGAAAGCG GCGAAATACG GTGCTGTTCC GGGTGTACTG 1620

GGCGGTCTGG GTGCTCTGGG CGGTGTTGGT ATCCCGGGCG GTGTTGTAGG TGCAGGCCCA 1680

GCTGCAGCTG CTGCTGCGGC AAAGGCAGCG GCGAAAGCAG CTCAGTTCGG TCTGGTTGGT 1740

GCAGCAGGTC TGGGCGGTCT GGGTGTTGGC GGTCTGGGTG TACCGGGCGT TGGTGGTCTG 1800

GGTGGCATCC CGCCGGCGGC GGCAGCTAAA GCGGCTAAAT ACGGTGCAGC AGGTCTGGGT 1860

GGCGTTCTGG GTGGTGCTGG TCAGTTCCCA CTGGGCGGTG TAGCGGCACG TCCGGGTTTC 1920

GGTCTGTCCC CGATCTTCCC AGGCGGTGCA TGCCTGGGTA AAGCTTGCGG CCGTAAACGT 1980

AAA 1983

(2) INFORMATION FOR SEQ ID NO : 5 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 660 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 5:

Met Gly Gly Val Pro Gly Ala Val Pro Gly Gly Val Pro Gly Gly Val 1 5 10 15

Phe Tyr Pro Gly Ala Gly Phe Gly Ala Val Pro Gly Gly Val Ala Asp 20 25 30

Ala Ala Ala Ala Tyr Lys Ala Ala Lys Ala Gly Ala Gly Leu Gly Gly 35 40 45

Val Pro Gly Val Gly Gly Leu Gly Val Ser Ala Gly Ala Val Val Pro 50 55 60 Gin Pro Gly Ala Gly Val Lys Pro Gly Lys Val Pro Gly Val Gly Leu 65 70 75 80

Pro Gly Val Tyr Pro Gly Phe Gly Ala Val Pro Gly Ala Arg Phe Pro 85 90 95

Gly Val Gly Val Leu Pro Gly Val Pro Thr Gly Ala Gly Val Lys Pro 100 105 110

Lys Ala Pro Gly Val Gly Gly Ala Phe Ala Gly He Pro Gly Val Gly 115 120 125

Pro Phe Gly Gly Pro Gin Pro Gly Val Pro Leu Gly Tyr Pro He Lys 130 135 140

Ala Pro Lys Leu Pro Gly Gly Tyr Gly Leu Pro Tyr Thr Thr Gly Lys

145 150 155 160

Leu Pro Tyr Gly Tyr Gly Pro Gly Gly Val Ala Ala Ala Gly Lys Ala 165 170 175

Gly Tyr Pro Thr Gly Thr Gly Val Gly Pro Gin Ala Ala Ala Ala Ala 180 185 190

Ala Ala Lys Ala Ala Ala Lys Phe Gly Ala Gly Ala Ala Gly Phe Gly 195 200 205

Ala Val Pro Gly Val Gly Gly Ala Gly Val Pro Gly Val Pro Gly Ala 210 215 220

He Pro Gly He Gly Gly He Ala Gly Val Gly Thr Pro Ala Ala Ala 225 230 235 240

Ala Ala Ala Ala Ala Ala Ala Lys Ala Ala Lys Tyr Gly Ala Ala Ala 245 250 255

Gly Leu Val Pro Gly Gly Pro Gly Phe Gly Pro Gly Val Val Gly Val 260 265 270

Pro Gly Phe Gly Ala Val Pro Gly Val Gly Val Pro Gly Ala Gly He 275 280 285

Pro Val Val Pro Gly Ala Gly He Pro Gly Ala Ala Gly Phe Gly Ala 290 295 300

Val Ser Pro Glu Ala Ala Ala Lys Ala Ala Ala Lys Ala Ala Lys Tyr 305 310 315 320

Gly Ala Arg Pro Gly Val Gly Val Gly Gly He Pro Thr Tyr Gly Val 325 330 335

Gly Ala Gly Phe Phe Pro Gly Phe Gly Val Gly Val Gly Gly He Pro 340 345 350

Gly Val Ala Gly Val Pro Ser Val Gly Gly Val Pro Gly Val Gly Gly 355 360 365

Val Pro Gly Val Gly He Ser Pro Glu Ala Gin Ala Ala Ala Ala Ala 370 375 380

Lys Ala Ala Lys Tyr Gly Val Gly Thr Pro Ala Ala Ala Ala Ala Lys 385 390 395 400

Ala Ala Ala Lys Ala Ala Gin Phe Gly Leu Val Pro Gly Val Gly Val 405 410 415

Ala Pro Gly Val Gly Val Ala Pro Gly Val Gly Val Ala Pro Gly Val 420 425 430

Gly Leu Ala Pro Gly Val Gly Val Ala Pro Gly Val Gly Val Ala Pro 435 440 445

Gly Val Gly Val Ala Pro Gly He Gly Pro Gly Gly Val Ala Ala Ala 450 455 460

Ala Lys Ser Ala Ala Lys Val Ala Ala Lys Ala Gin Leu Arg Ala Ala 465 470 475 480

Ala Gly Leu Gly Ala Gly He Pro Gly Leu Gly Val Gly Val Gly Val 485 490 495

Pro Gly Leu Gly Val Gly Ala Gly Val Pro Gly Leu Gly Val Gly Ala 500 505 510

Gly Val Pro Gly Phe Gly Ala Val Pro Gly Ala Leu Ala Ala Ala Lys 515 520 525

Ala Ala Lys Tyr Gly Ala Val Pro Gly Val Leu Gly Gly Leu Gly Ala 530 535 540

Leu Gly Gly Val Gly He Pro Gly Gly Val Val Gly Ala Gly Pro Ala 545 550 555 560 Ala Ala Ala Ala Ala Ala Lys Ala Ala Ala Lys Ala Ala Gin Phe Gly 565 570 575

Leu Val Gly Ala Ala Gly Leu Gly Gly Leu Gly Val Gly Gly Leu Gly 580 585 590

Val Pro Gly Val Gly Gly Leu Gly Gly He Pro Pro Ala Ala Ala Ala 595 600 605

Lys Ala Ala Lys Tyr Gly Ala Ala Gly Leu Gly Gly Val Leu Gly Gly 610 615 620

Ala Gly Gin Phe Pro Leu Gly Gly Val Ala Ala Arg Pro Gly Phe Gly 625 630 635 640

Leu Ser Pro He Phe Pro Gly Gly Ala Cys Leu Gly Lys Ala Cys Gly 645 650 655

Arg Lys Arg Lys 660

(2 ) INFORMATION FOR SEQ ID NO: 6 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 441 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: double

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: DNA (genomic)

(iii) HYPOTHETICAL: YES

(iv) ANTI-SENSE: NO

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6:

TCCGCCATGG GAGGTGTTCC GGGCGCGCTG GCTGCTGCGA AAGCGGCGAA ATACGGTGCA 60

GCGGTTCCGG GTGTACTGGG CGGTCTGGGT GCTCTGGGCG GTGTTGGTAT CCCGGGCGGT 120

GTTGTAGGTG CAGGCCCAGC TGCAGCTGCT GCTGCGGCAA AGGCAGCGGC GAAAGCAGCT 180 CAGTTCGGTC TGGTTGGTGC AGCAGGTGTG GGCGGTCTGG GTGTTGGCGG TCTGGGTGTA 240

CCGGGCGTTG GTGGTCTGGG TGGCATCCCG CCGGCGGCGG CAGCTAAAGC GGCTAAATAC 300

GGTGCAGCAG GTCTGGGTGG CGTTCTGGGT GGTGCTGGTC AGTTCCCACT GGGCGGTGTA 360

GCGGCACGTC CGGGTTTCGG TCTGTCCCCG ATCTTCCCAG GCGGTGCATG CCTGGGTAAA 420

GCTTGCGGCC GTAAACGTAA A 441

(2) INFORMATION FOR SEQ ID NO : 7 :

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 147 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO : 7 :

Ser Ala Met Gly Gly Val Pro Gly Ala Leu Ala Ala Ala Lys Ala Ala 1 5 10 15

Lys Tyr Gly Ala Ala Val Pro Gly Val Leu Gly Gly Leu Gly Ala Leu 20 25 30

Gly Gly Val Gly He Pro Gly Gly Val Val Gly Ala Gly Pro Ala Ala 35 40 45

Ala Ala Ala Ala Ala Lys Ala Ala Ala Lys Ala Ala Gin Phe Gly Leu 50 55 60

Val Gly Ala Ala Gly Leu Gly Gly Leu Gly Val Gly Gly Leu Gly Val 65 70 75 80

Pro Gly Val Gly Gly Leu Gly Gly He Pro Pro Ala Ala Ala Ala Lys 85 90 95

Ala Ala Lys Tyr Gly Ala Ala Gly Leu Gly Gly Val Leu Gly Gly Ala 100 105 110 Gly Gin Phe Pro Leu Gly Gly Val Ala Ala Arg Pro Gly Phe Gly Leu 115 120 125

Ser Pro He Phe Pro Gly Gly Ala Cys Leu Gly Lys Ala Cys Gly Arg 130 135 140

Lys Arg Lys 145

(2) INFORMATION FOR SEQ ID NO: 8:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 600 base pairs

(B) TYPE: nucleic acid

(C) STRANDEDNESS: double

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: DNA (genomic)

(iii) HYPOTHETICAL: YES

(iv) ANTI-SENSE: NO

(xi) SEQUENCE DESCRIPTION: SEQ ID NO : 8 :

TCCGCCATGG GAGCTCTGGT AGGCCTGGGC GTACCGGGCC TGGGTGTTGG TGCAGGCGTT 60

CCGGGTTTCG GTGCTGGCGC GGACGAAGGT GTACGTCGTT CCCTGTCTCC AGAACTGCGT 120

GAAGGTGACC CGTCCTCTTC CCAGCACCTG CCGTCTACCC CGTCCTCTCC ACGTGTTCCG 180

GGCGCGCTGG CTGCTGCGAA AGCGGCGAAA TACGGTGCAG CGGTTCCGGG TGTACTGGGC 240

GGTCTGGGTG CTCTGGGCGG TGTTGGTATC CCGGGCGGTG TTGTAGGTGC AGGCCCAGCT 300

GCAGCTGCTG CTGCGGCAAA GGCAGCGGCG AAAGCAGCTC AGTTCGGTCT GGTTGGTGCA 360

GCAGGTCTGG GCGGTCTGGG TGTTGGCGGT CTGGGTGTAC CGGGCGTTGG TGGTCTGGGT 420

GGCATCCCGC CGGCGGCGGC AGCTAAAGCG GCTAAATACG GTGCAGCAGG TCTGGGTGGC 480

GTTCTGGGTG GTGCTGGTCA GTTCCCACTG GGCGGTGTAG CGGCACGTCC GGGTTTCGGT 540 CTGTCCCCGA TCTTCCCAGG CGGTGCATGC CTGGGTAAAG CTTGCGGCCG TAAACGTAAA 600

(2) INFORMATION FOR SEQ ID NO: 9:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 200 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO : 9 :

Ser Ala Met Gly Ala Leu Val Gly Leu Gly Val Pro Gly Leu Gly Val 1 5 10 15

Gly Ala Gly Val Pro Gly Phe Gly Ala Gly Ala Asp Glu Gly Val Arg 20 25 30

Arg Ser Leu Ser Pro Glu Leu Arg Glu Gly Asp Pro Ser Ser Ser Gin 35 40 45

His Leu Pro Ser Thr Pro Ser Ser Pro Arg Val Pro Gly Ala Leu Ala 50 55 60

Ala Ala Lys Ala Ala Lys Tyr Gly Ala Ala Val Pro Gly Val Leu Gly 65 70 75 80

Gly Leu Gly Ala Leu Gly Gly Val Gly He Pro Gly Gly Val Val Gly 85 90 95

Ala Gly Pro Ala Ala Ala Ala Ala Ala Ala Lys Ala Ala Ala Lys Ala 100 105 110

Ala Gin Phe Gly Leu Val Gly Ala Ala Gly Leu Gly Gly Leu Gly Val 115 120 125

Gly Gly Leu Gly Val Pro Gly Val Gly Gly Leu Gly Gly He Pro Pro 130 135 140 Ala Ala Ala Ala Lys Ala Ala Lys Tyr Gly Ala Ala Gly Leu Gly Gly 145 150 155 160

Val Leu Gly Gly Ala Gly Gin Phe Pro Leu Gly Gly Val Ala Ala Arg 165 170 175

Pro Gly Phe Gly Leu Ser Pro He Phe Pro Gly Gly Ala Cys Leu Gly 180 185 190

Lys Ala Cys Gly Arg Lys Arg Lys 195 200

(2) INFORMATION FOR SEQ ID NO: 10:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 60 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10:

Gly He Pro Pro Ala Ala Ala Ala Lys Ala Ala Lys Tyr Gly Ala Ala 1 5 10 15

Gly Leu Gly Gly Val Leu Gly Gly Ala Gly Gin Phe Pro Leu Gly Gly 20 25 30

Val Ala Ala Arg Pro Gly Phe Gly Leu Ser Pro He Phe Pro Gly Gly 35 40 45

Ala Cys Leu Gly Lys Ala Cys Gly Arg Lys Arg Lys 50 55 60

(2) INFORMATION FOR SEQ ID NO: 11:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 47 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11:

Gly Ala Ala Gly Leu Gly Gly Val Leu Gly Gly Ala Gly Gin Phe Pro 1 5 10 15

Leu Gly Gly Val Ala Ala Arg Pro Gly Phe Gly Leu Ser Pro He Phe 20 25 30

Pro Gly Gly Ala Cys Leu Gly Lys Ala Cys Gly Arg Lys Arg Lys 35 40 45

(2) INFORMATION FOR SEQ ID NO: 12:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 34 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 12:

Gly Ala Asp Glu Gly Val Arg Arg Ser Leu Ser Pro Glu Leu Arg Glu 1 5 10 15

Gly Asp Pro Ser Ser Ser Gin His Leu Pro Ser Thr Pro Ser Ser Pro 20 25 30

Arg Val

(2) INFORMATION FOR SEQ ID NO: 13:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 34 amino acids

(B) TYPE: amino acid (C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13:

Gly Ala Asp Glu Gly Val Arg Arg Ser Leu Ser Pro Glu Leu Arg Glu 1 5 10 15

Gly Asp Pro Ser Ser Ser Gin His Leu Pro Ser Thr Pro Ser Ser Pro 20 25 30

Arg Phe

(2) INFORMATION FOR SEQ ID NO: 14:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 216 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14:

Ala Ala Ala Gly Leu Gly Ala Gly He Pro Gly Leu Gly Val Gly Val

1 5 10 15

Gly Val Pro Gly Leu Gly Val Gly Ala Gly Val Pro Gly Leu Gly Val 20 25 30

Gly Ala Gly Val Pro Gly Phe Gly Ala Gly Ala Asp Glu Gly Val Arg 35 40 45

Arg Ser Leu Ser Pro Glu Leu Arg Glu Gly Asp Pro Ser Ser Ser Gin 50 55 60 His Leu Pro Ser Thr Pro Ser Ser Pro Arg Val Pro Gly Ala Leu Ala 65 70 75 80

Ala Ala Lys Ala Ala Lys Tyr Gly Ala Ala Val Pro Gly Val Leu Gly 85 90 95

Gly Leu Gly Ala Leu Gly Gly Val Gly He Pro Gly Gly Val Val Gly 100 105 110

Ala Gly Pro Ala Ala Ala Ala Ala Ala Ala Lys Ala Ala Ala Lys Ala 115 120 125

Ala Gin Phe Gly Leu Val Gly Ala Ala Gly Leu Gly Gly Leu Gly Val 130 135 140

Gly Gly Leu Gly Val Pro Gly Val Gly Gly Leu Gly Gly He Pro Pro 145 150 155 160

Ala Ala Ala Ala Lys Ala Ala Lys Tyr Gly Ala Ala Gly Leu Gly Gly 165 170 175

Val Leu Gly Gly Ala Gly Gin Phe Pro Leu Gly Gly Val Ala Ala Arg 180 185 190

Pro Gly Phe Gly Leu Ser Pro He Phe Pro Gly Gly Ala Cys Leu Gly 195 200 205

Lys Ala Cys Gly Arg Lys Arg Lys 210 215

(2) INFORMATION FOR SEQ ID NO: 15:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 183 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: protein

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 15:

Ala Ala Ala Gly Leu Gly Ala Gly He Pro Gly Leu Gly Val Gly Val 1 5 10 15

Gly Val Pro Gly Leu Gly Val Gly Ala Gly Val Pro Gly Leu Gly Val 20 25 30

Gly Ala Gly Val Pro Gly Phe Gly Ala Val Pro Gly Ala Leu Ala Ala 35 40 45

Ala Lys Ala Ala Lys Tyr Gly Ala Ala Val Pro Gly Val Leu Gly Gly 50 55 60

Leu Gly Ala Leu Gly Gly Val Gly He Pro Gly Gly Val Val Gly Ala 65 70 75 80

Gly Pro Ala Ala Ala Ala Ala Ala Ala Lys Ala Ala Ala Lys Ala Ala 85 90 95

Gin Phe Gly Leu Val Gly Ala Ala Gly Leu Gly Gly Leu Gly Val Gly 100 105 110

Gly Leu Gly Val Pro Gly Val Gly Gly Leu Gly Gly He Pro Pro Ala 115 120 125

Ala Ala Ala Lys Ala Ala Lys Tyr Gly Ala Ala Gly Leu Gly Gly Val 130 135 140

Leu Gly Gly Ala Gly Gin Phe Pro Leu Gly Gly Val Ala Ala Arg Pro 145 150 155 160

Gly Phe Gly Leu Ser Pro He Phe Pro Gly Gly Ala Cys Leu Gly Lys 165 170 175

Ala Cys Gly Arg Lys Arg Lys 180

Claims

THE CLAIMS :

1. A human tropoelastin derivative or an amino acid sequence variant thereof, wherein the derivative or variant has elastin-like properties.

2. A human tropoelastin derivative or an amino acid sequence variant thereof, wherein the derivative or variant has macro-molecular binding properties .

3. A derivative or variant thereof according to claim 2 wherein the macro-molecular binding properties include the ability to bind glycosyaminoglycans .

4. A human tropoelastin derivative or an amino acid sequence variant thereof, wherein the derivative or variant has elastin-like properties and macro-molecular binding properties .

5. A polynucleotide encoding a derivative or variant thereof of any one of claims 1 to 4.

6. A tropoelastin derivative comprising the amino acid sequence of SHEL╬┤modified, or an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL╬┤modified.

7. A tropoelastin derivative according to claim 6 comprising SEQ ID NO: 5.

8. A polynucleotide encoding a tropoelastin derivative, the derivative comprising the amino acid sequence of SHEL╬┤modified or an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL╬┤modified.

9. A polynucleotide according to claim 8 comprising SEQ ID NO: 4.

10. A synthetic polynucleotide encoding a tropoelastin derivative, the derivative comprising the amino acid sequence of SHEL╬┤26A or an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL╬┤26A.

11. A synthetic polynucleotide according to claim 10, the polynucleotide comprising the sequence of from nucleotide position 1 to 1676 contiguous with the sequence of from nucleotide position 1775 to 2210 of SEQ ID NO : 1.

12. An amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL╬┤26A.

13. An amino acid sequence variant according to claim 12 comprising SEQ ID NO : 3.

14. A tropoelastin derivative comprising the amino acid sequence of SHELgamma, or an amino acid sequence variant of the derivative comprising the amino acid sequence of SHELgamma.

15. A tropoelastin derivative according to claim 14 comprising SEQ ID NO: 9.

16. A polynucleotide encoding a tropoelastin derivative, the derivative comprising the amino acid sequence of the derivative SHELgamma, or an amino acid sequence variant of the derivative comprising the amino acid sequence of SHELgamma.

17. A polynucleotide sequence according to claim 16 comprising SEQ ID NO: 8.

18. A tropoelastin derivative comprising the amino acid sequence of SHELgamma excluding exon 26A, or an amino acid sequence variant of the derivative comprising the amino acid sequence of SHELgamma excluding exon 26A.

19. A tropoelastin derivative according to claim 18 comprising SEQ ID NO: 7.

20. A polynucleotide encoding a tropoelastin derivative, the derivative comprising the amino acid sequence of SHELgamma excluding exon 26A or an amino acid sequence variant of the derivative comprising the amino acid sequence of SHELgamma excluding exon 26A.

21. A polynucleotide sequence according to claim 20 comprising SEQ ID NO: 6.

22. A tropoelastin derivative comprising the amino acid sequence of SHEL31-36, or an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL31-36.

23. A tropoelastin derivative according to claim 22 comprising SEQ ID NO: 10.

24. A polynucleotide encoding a tropoelastin derivative, the derivative comprising the amino acid sequence of SHEL31-36 or an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL31-36.

25. A polynucleotide according to claim 24, the polynucleotide comprising the sequence of from nucleotide position 2022 to 2210 of SEQ ID NO: 1.

26. A tropoelastin derivative comprising the amino acid sequence of SHEL32-36, or an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL32-36.

27. A tropoelastin derivative according to claim 26 comprising SEQ ID NO: 11.

28. A polynucleotide encoding a tropoelastin derivative, the derivative comprising the amino acid sequence of SHEL32-36 or an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL32-36.

29. A polynucleotide according to claim 28, the polynucleotide comprising the sequence of from nucleotide position 2061 to 2210 of SEQ ID NO: 1.

30. A tropoelastin derivative comprising the amino acid sequence of peptide 26A, or an amino acid sequence variant of the derivative comprising the amino acid sequence of peptide 26A.

31. A tropoelastin derivative according to claim 30 comprising SEQ ID NO: 12 or SEQ ID NO: 13.

32. A polynucleotide encoding a tropoelastin derivative, the derivative comprising the amino acid sequence of peptide 26A or an amino acid sequence variant of the derivative comprising the amino acid sequence of peptide 26A.

33. A polynucleotide according to claim 32, the polynucleotide comprising the sequence of from nucleotide position 1677 to 1774 of SEQ ID NO: 1.

34. A tropoelastin derivative comprising the amino acid sequence of SHEL26-36, or an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL26-26.

35. A tropoelastin derivative according to claim 34 comprising SEQ ID NO: 14.

36. A polynucleotide encoding a tropoelastin derivative, the derivative comprising the amino acid sequence of SHEL26-36 or an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL26-36.

37. A polynucleotide according to claim 36, the polynucleotide comprising the sequence of from nucleotide position 1554 to 2210 of SEQ ID NO: 1.

38. A tropoelastin derivative comprising the amino acid sequence of SHEL26-26 excluding exon 26A, or an amino acid sequence variant of the derivative comprising the amino acid sequence of SHEL26-26 excluding exon 26A.

39. A tropoelastin derivative according to claim 38 comprising SEQ ID NO: 15.

40. A polynucleotide encoding a tropoelastin derivative, the derivative comprising the amino acid sequence of SHEL26-26 excluding exon 26A or an amino acid sequence variant of the derivative of SHEL26-26 excluding exon 26A.

41. A polynucleotide according to claim 40, the polynucleotide comprising the sequence of from nucleotide position 1554 to 1676 contiguous with the sequence of from nucleotide position 1776 to 2210 of SEQ ID NO: 1.

42. A vector comprising a polynucleotide according to any one of claims 5, 8, 9, 16, 17, 20, 21, 24, 25, 28, 29, 32, 33, 36, 37, 40 or 41, or a synthetic polynucleotide according to claim 10 or 11.

43. The vector according to claim 42 wherein the polynucleotide or synthetic polynucleotide is operatively linked to a promoter or enhancer regulatory sequence.

44. The vector according to claim 42 or 43 wherein the polynucleotide or synthetic polynucleotide is operatively linked to a nucleotide sequence, the nucleotide sequence encoding a further amino acid sequence .

45. A cell containing a vector according to any one of claims 42 to 44.

46. A method for producing a derivative of tropoelastin or an amino acid sequence variant of the derivative, the method comprising:

(a) providing a vector according to any one of claims 42 to 44;

(b) introducing the vector into a cell;

(c) maintaining the cell in conditions suitable for expression of the vector; and

(d) isolating the tropoelastin derivative or variant .

47. A tropoelastin derivative or variant produced by the method of claim 46.

48. A transgenic non-human animal containing a vector according to any one of claims 42 to 44, or a polynucleotide according to any one of claims 5, 8, 9, 16, 17, 20, 21, 24, 25, 28, 29, 32, 33, 36, 37, 40 or 41, or a synthetic polynucleotide according to claim 10 or 11.

49. A tropoelastin derivative or variant of the derivative produced by a transgenic animal according to claim 48

50. method for producing a tropoelastin derivative or a variant of the derivative according to any one of claims 1-4, 6, 7, 12-15, 18, 19, 22, 23, 26, 27, 30, 31, 34, 35, 38 or 39, the method comprising producing the tropoelastin derivative or variant by solid-phase peptide synthesis .

51. A tropoelastin derivative or variant produced by the method of claim 50.

52. A formulation comprising at least one tropoelastin derivative or variant of the derivative according to any one of 1-4, 6, 7, 12-15, 18, 19, 22, 23, 26, 27, 30, 31, 34, 35, 38, 39, 47 or 49, together with a pharmaceutically acceptable carrier or diluent.

53. An expression product comprising a tropoelastin derivative or variant of the derivative according to any one of claims 1-4, 6, 7, 12-15, 18, 19, 22, 23, 26, 27, 30, 31, 34, 35, 38, 39, 47 or 49, and a further amino acid sequence .

54. An expression product according to claim 53 wherein the tropoelastin derivative comprises the amino acid sequence of peptide 26A, or an amino acid sequence variant of the derivative comprising the amino acid sequence of peptide 26A.

55. A polynucleotide encoding an expression product according to claims 53 or 54.

56. A vector comprising the polynucleotide according to claim 55.

57. A cell containing a vector according to claim 56.

58. A method for producing an expression product according to claim 52 or 54, the method comprising:

(a) providing a vector according to claim 56; (b) introducing the vector into a cell;

(d) isolating the expression product.

59. An expression product produced by the method of claim 58.

60. An transgenic non-human animal containing a vector according to claim 56 or a polynucleotide according to claim 55.

61. An expression product produced by a transgenic animal according to claim 60.

62. A formulation comprising at least one expression product according to any of claims 53, 54, 59 or 61, together with a pharmaceutically acceptable carrier or diluent .

63. A hybrid molecule comprising a biological polymer wherein the polymer is linked to a tropoelastin derivative comprising the amino acid sequence of peptide 26A or an amino acid sequence variant of the derivative comprising peptide 26A.

64. A hybrid molecule according to claim 63 wherein the biological polymer is a protein.

65. A hybrid molecule according to claim 64 wherein in the protein is selected from the group consisting of cytokines, growth factors and antibodies.

66. A hybrid molecule according to claim 63 wherein the biological polymer is selected from the group consisting of lipids, sugars and nucleic acids.

67. A polynucleotide sequence encoding a hybrid molecule according to claim 64.

68. A vector comprising a polynucleotide sequence according to claim 67.

69. A cell containing a vector according to claim 68.

70. A method for producing a hybrid molecule according to claim 64, the method comprising: (a) providing a vector according to claim 68;

(b) introducing the vector into a cell;

(d) isolating the hybrid molecule.

71. A hybrid molecule produced by the method of claim 70.

72. A transgenic non-human animal containing a vector according to claim 68 or a polynucleotide according to claim 67.

73. A hybrid molecule produced by a transgenic animal according to claim 72.

74. A hybrid molecule comprising a synthetic polymer linked to peptide 26A or a variant of peptide 26A.

75. A formulation comprising at least one hybrid molecule according to any of claims 63-65, 71, 73 and 74, together with a pharmaceutically acceptable carrier or diluent .

76. A cross linked complex, the complex comprising at least one of the following:

(i) at least one derivative or variant of the derivative according to any of 1-4, 6, 7, 12-15, 18, 19, 22, 23, 26, 27, 30, 31, 34, 35, 38, 39, 47 or 49; (ii) at least expression product according to any of claims 53, 54, 58 or 61; and (iii) at least one hybrid molecule according to any of claims 63-65, 71, 73 or 74.

77. An implant, the implant comprising at least one of the following:

(i) at least one derivative or variant of the derivative according to any of 1-4, 6, 7, 12-15,

18, 19, 22, 23, 26, 27, 30, 31, 34, 35, 38, 39, 47 or 49; (ii) at least expression product according to any of claims 53, 54, 58 or 61; and (iii) at least one hybrid molecule according to any of claims 63-65, 71, 73 or 74.

78. A method of imparting glycosaminoglycan binding activity to a biological polymer comprising the step of linking a tropoelastin derivative comprising the amino acid sequence of peptide 26A, or an amino acid sequence variant of the derivative comprising the amino acid sequence of peptide 26A with the biological polymer.

79. A method of deleting glycosaminoglycan binding activity from a biological polymer comprising the step of deleting a tropoelastin derivative comprising the amino acid sequence of peptide 26A, or an amino acid sequence variant of the derivative comprising the amino acid sequence of peptide 26A from the biological polymer.

80. The method of claim 66 or 67 wherein the biological polymer is a protein.

81. A formulation comprising a tropoelastin derivative or variant of the derivative and a synthetic or biological polymer.