WO1998046770A3 - Stable biocatalysts for ester hydrolysis - Google Patents

Stable biocatalysts for ester hydrolysis Download PDF

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Publication number
WO1998046770A3
WO1998046770A3 PCT/US1998/007237 US9807237W WO9846770A3 WO 1998046770 A3 WO1998046770 A3 WO 1998046770A3 US 9807237 W US9807237 W US 9807237W WO 9846770 A3 WO9846770 A3 WO 9846770A3
Authority
WO
WIPO (PCT)
Prior art keywords
stable
ester hydrolysis
biocatalysts
stable biocatalysts
encode
Prior art date
Application number
PCT/US1998/007237
Other languages
French (fr)
Other versions
WO1998046770A2 (en
WO1998046770A9 (en
Inventor
Larry Allen
John Aikens
Michael Fonstein
Veronika Vonstein
David Demirjian
Malcolm Casadaban
Original Assignee
Thermogen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Thermogen Inc filed Critical Thermogen Inc
Priority to JP10544106A priority Critical patent/JP2000511437A/en
Priority to CA002286481A priority patent/CA2286481A1/en
Priority to AU71086/98A priority patent/AU7108698A/en
Priority to EP98918096A priority patent/EP1005556A2/en
Publication of WO1998046770A2 publication Critical patent/WO1998046770A2/en
Publication of WO1998046770A3 publication Critical patent/WO1998046770A3/en
Publication of WO1998046770A9 publication Critical patent/WO1998046770A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The instant invention encompasses isolated stable esterase enzymes characterized by the ability to remain stable at certain temperatures, substrate specificities, and activity profile; the expression vectors which can express, nucleic acids which encode for, and corresponding protein amino acid sequence of such proteins.
PCT/US1998/007237 1997-04-11 1998-04-10 Stable biocatalysts for ester hydrolysis WO1998046770A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP10544106A JP2000511437A (en) 1997-04-11 1998-04-10 A stable biocatalyst for ester hydrolysis
CA002286481A CA2286481A1 (en) 1997-04-11 1998-04-10 Stable biocatalysts for ester hydrolysis
AU71086/98A AU7108698A (en) 1997-04-11 1998-04-10 Stable biocatalysts for ester hydrolysis
EP98918096A EP1005556A2 (en) 1997-04-11 1998-04-10 Stable biocatalysts for ester hydrolysis

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US82781097A 1997-04-11 1997-04-11
US08/827,810 1997-04-11

Publications (3)

Publication Number Publication Date
WO1998046770A2 WO1998046770A2 (en) 1998-10-22
WO1998046770A3 true WO1998046770A3 (en) 1998-11-26
WO1998046770A9 WO1998046770A9 (en) 1999-03-18

Family

ID=25250233

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/007237 WO1998046770A2 (en) 1997-04-11 1998-04-10 Stable biocatalysts for ester hydrolysis

Country Status (5)

Country Link
EP (1) EP1005556A2 (en)
JP (1) JP2000511437A (en)
AU (1) AU7108698A (en)
CA (1) CA2286481A1 (en)
WO (1) WO1998046770A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001238407A1 (en) * 2000-02-16 2001-08-27 Thermogen, Inc. Esterase enzymes having selective activity

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0024182A2 (en) * 1979-08-08 1981-02-25 Development Finance Corporation Of New Zealand Thermo-stable micro-organism and proteolytic enzyme prepared therefrom, and processes for the preparation of the micro-organism and the proteolytic enzyme
WO1997025058A1 (en) * 1996-01-11 1997-07-17 Thermogen, Inc. Stable biocatalysts for ester hydrolysis

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0024182A2 (en) * 1979-08-08 1981-02-25 Development Finance Corporation Of New Zealand Thermo-stable micro-organism and proteolytic enzyme prepared therefrom, and processes for the preparation of the micro-organism and the proteolytic enzyme
WO1997025058A1 (en) * 1996-01-11 1997-07-17 Thermogen, Inc. Stable biocatalysts for ester hydrolysis

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
APPL. MICROBIOL. BIOTECHNOL. (1995), 44(1-2), 81-7 CODEN: AMBIDG;ISSN: 0175-7598 *
CHEMICAL ABSTRACTS, vol. 115, no. 23, 9 December 1991, Columbus, Ohio, US; abstract no. 250809, SILVA, A. M. G. M. ET AL: "Characteristics of a new lipase from a Thermus sp bacterium" XP002079272 *
CHEMICAL ABSTRACTS, vol. 119, no. 1, 5 July 1993, Columbus, Ohio, US; abstract no. 3601, FUKATSU, MAKOTO ET AL: "Purification and properties of lipase derived from the extreme thermophile, Thermus thermophilus 111" XP002079273 *
CHEMICAL ABSTRACTS, vol. 125, no. 1, 1 July 1996, Columbus, Ohio, US; abstract no. 4153, BERGER, J.-L. ET AL: "Identification of new enzyme activities of several strains of Thermus species" XP002079270 *
CHEMICAL ABSTRACTS, vol. 126, no. 15, 14 April 1997, Columbus, Ohio, US; abstract no. 196671, BUNPUAN, KANOKPORN ET AL: "Partial purification and characterization of acid resistant lipases from Thermus T20" XP002079271 *
GBF MONOGR. (1991), 16(LIPASES), 417-20 CODEN: GBMOEB *
J. SCI. FAC. CHIANG MAI UNIV. (1995), 21-22(1-2), 17-20 CODEN: JSFUD9;ISSN: 0125-2526 *
SIGURGISLADOTTIR, SJOFN ET AL: "Lipase activity of thermophilic bacteria from Icelandic hot springs", BIOTECHNOL. LETT. (1993), 15(4), 361-6 CODEN: BILED3;ISSN: 0141-5492, XP002079269 *
YUKAGAKU (1993), 42(3), 179-83 CODEN: YKGKAM;ISSN: 0513-398X *

Also Published As

Publication number Publication date
WO1998046770A2 (en) 1998-10-22
AU7108698A (en) 1998-11-11
CA2286481A1 (en) 1998-10-22
EP1005556A2 (en) 2000-06-07
JP2000511437A (en) 2000-09-05

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