WO1998038285A3 - Mutant carboxypeptidase y and methods for transamidation - Google Patents

Mutant carboxypeptidase y and methods for transamidation Download PDF

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Publication number
WO1998038285A3
WO1998038285A3 PCT/IB1998/000517 IB9800517W WO9838285A3 WO 1998038285 A3 WO1998038285 A3 WO 1998038285A3 IB 9800517 W IB9800517 W IB 9800517W WO 9838285 A3 WO9838285 A3 WO 9838285A3
Authority
WO
WIPO (PCT)
Prior art keywords
carboxypeptidase
transamidation
methods
amino acid
subsite
Prior art date
Application number
PCT/IB1998/000517
Other languages
French (fr)
Other versions
WO1998038285A2 (en
Inventor
Uffe Mortensen
Kjeld Olesen
Henning Stennicke
Steen B Soerensen
Klaus Breddam
Original Assignee
Carlsberg Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carlsberg Lab filed Critical Carlsberg Lab
Priority to JP53746298A priority Critical patent/JP2001513638A/en
Priority to EP98910909A priority patent/EP0966526A2/en
Priority to AU65133/98A priority patent/AU740742B2/en
Priority to CA002281879A priority patent/CA2281879A1/en
Publication of WO1998038285A2 publication Critical patent/WO1998038285A2/en
Publication of WO1998038285A3 publication Critical patent/WO1998038285A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

A method for transamidating a peptide substrate having a P1 amino acid residue with a positively charged side chain. According to the invention, carboxypeptidase Y is modified to substitute at least one amino acid having a negatively charged side chain in an S1 subsite. Additionally, the modified carboxypeptidase Y can include substituted amino acid residues in an S1', S2 and/or S3 subsite to accommodate a specific peptide substrate.
PCT/IB1998/000517 1997-02-28 1998-02-27 Mutant carboxypeptidase y and methods for transamidation WO1998038285A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP53746298A JP2001513638A (en) 1997-02-28 1998-02-27 Modified carboxypeptidase
EP98910909A EP0966526A2 (en) 1997-02-28 1998-02-27 Modified carboxypeptidase
AU65133/98A AU740742B2 (en) 1997-02-28 1998-02-27 Modified carboxypeptidase
CA002281879A CA2281879A1 (en) 1997-02-28 1998-02-27 Modified carboxypeptidase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/807,263 US5985627A (en) 1997-02-28 1997-02-28 Modified carboxypeptidase
US08/807,263 1997-02-28

Publications (2)

Publication Number Publication Date
WO1998038285A2 WO1998038285A2 (en) 1998-09-03
WO1998038285A3 true WO1998038285A3 (en) 1999-01-07

Family

ID=25195962

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB1998/000517 WO1998038285A2 (en) 1997-02-28 1998-02-27 Mutant carboxypeptidase y and methods for transamidation

Country Status (7)

Country Link
US (2) US5985627A (en)
EP (1) EP0966526A2 (en)
JP (1) JP2001513638A (en)
CN (1) CN1252836A (en)
AU (1) AU740742B2 (en)
CA (1) CA2281879A1 (en)
WO (1) WO1998038285A2 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9323429D0 (en) * 1993-11-12 1994-01-05 Wellcome Found Therapy
US6436691B1 (en) * 1995-08-16 2002-08-20 Zeneca Limited Chemical compounds
DE10047857B4 (en) * 2000-09-27 2004-11-18 Universität Leipzig Process for the selective biocatalytic modification of peptides and proteins
WO2003099852A2 (en) * 2002-05-24 2003-12-04 Restoragen Inc. Peptide derivatization process
US7524813B2 (en) 2003-10-10 2009-04-28 Novo Nordisk Health Care Ag Selectively conjugated peptides and methods of making the same
ES2309785T3 (en) 2004-08-13 2008-12-16 F. Hoffmann-La Roche Ag MODIFICATION C-TERMINAL OF POLYPEPTIDES.
EP1794294B1 (en) * 2004-09-27 2011-07-20 Sanofi-Aventis Deutschland GmbH Recombinant carboxypeptidase b
US20090105134A1 (en) 2005-02-10 2009-04-23 Novo Nordisk A/S C-Terminally Pegylated Growth Hormones
EP2217602B1 (en) 2007-11-30 2018-08-29 Boehringer Ingelheim International GmbH 1,5-dihydro-pyrazolo[3,4-d]pyrimidin-4-one derivatives and their use as pde9a modulators for the treatment of cns disorders
UA105362C2 (en) 2008-04-02 2014-05-12 Бьорингер Ингельхайм Интернациональ Гмбх 1-heterocyclyl-1, 5-dihydro-pyrazolo [3, 4-d] pyrimidin-4-one derivatives and their use as pde9a modulators
JP5977945B2 (en) 2008-08-06 2016-08-24 ノヴォ・ノルディスク・ヘルス・ケア・アーゲー Conjugate proteins with long-term in vivo efficacy
EA201100446A1 (en) 2008-09-08 2011-10-31 Бёрингер Ингельхайм Интернациональ Гмбх PYRAZOLOPYRIMIDINES AND THEIR APPLICATION FOR THE TREATMENT OF CNS DAMAGE
CN102292349B (en) 2009-01-22 2016-04-13 诺沃—诺迪斯克保健股份有限公司 Stable growth hormone compound
GEP20146098B (en) 2009-03-31 2014-05-27 Boehringer Ingelheim Int 1-heterocyclyl-1, 5-dihydro-pyrazolo [3, 4-d] pyrimidin-4-one derivatives and their usage as pde9a modulators
JP6086528B2 (en) 2009-08-06 2017-03-01 ノヴォ・ノルディスク・ヘルス・ケア・アーゲー Growth hormone with long-term in vivo efficacy
AU2011208625C1 (en) 2010-01-22 2022-08-18 Novo Nordisk Health Care Ag Growth hormones with prolonged in-vivo efficacy
RU2012134974A (en) 2010-01-22 2014-02-27 Ново Нордиск Хелс Кеа Аг STABILIZED GROWTH HORMONE COMPOUND
CN103052639B (en) 2010-08-12 2016-02-17 贝林格尔.英格海姆国际有限公司 6-cycloalkyl-1,5-dihydro-pyrazol is [3,4-d] pyrimidin-4-one-derivatives and the purposes as PDE9A inhibitor thereof also
US8809345B2 (en) 2011-02-15 2014-08-19 Boehringer Ingelheim International Gmbh 6-cycloalkyl-pyrazolopyrimidinones for the treatment of CNS disorders
CN105120887A (en) 2013-04-05 2015-12-02 诺和诺德保健股份有限公司 Growth hormone compound formulation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995020039A2 (en) * 1993-10-28 1995-07-27 Calsberg A/S Customized proteases with altered transacylation activity

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RO80806A (en) * 1979-04-06 1983-02-01 De Forenede Bryggerier As,Dk PROCEDURE FOR ENZYMATIC OBTAINING PEPTIDES
DK149824C (en) * 1982-01-22 1987-03-16 Carlsberg Biotechnology Ltd PROCEDURE FOR ENZYMATIC REPLACEMENT OF B-30 AMINO ACIDS IN INSULINES
US5185258A (en) * 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
JPH07121222B2 (en) * 1987-03-24 1995-12-25 寶酒造株式会社 Chemically modified serine protease-containing composition, its production method and use
US5403737A (en) * 1990-08-09 1995-04-04 Genentech, Inc. Serine protease variants having peptide ligase activity

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995020039A2 (en) * 1993-10-28 1995-07-27 Calsberg A/S Customized proteases with altered transacylation activity

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
OLESEN, K. ET AL.: "The activity of carboxypeptidase Y toward substrates with basic P1 amino acid residues ...", BIOCHEMISTRY, vol. 33, 1994, pages 11121 - 11126, XP002074788 *

Also Published As

Publication number Publication date
EP0966526A2 (en) 1999-12-29
AU740742B2 (en) 2001-11-15
JP2001513638A (en) 2001-09-04
US20010010923A1 (en) 2001-08-02
CA2281879A1 (en) 1998-09-03
AU6513398A (en) 1998-09-18
US5985627A (en) 1999-11-16
CN1252836A (en) 2000-05-10
WO1998038285A2 (en) 1998-09-03

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