WO1998031150A1 - Method and apparatus for monitoring a biological sample - Google Patents
Method and apparatus for monitoring a biological sample Download PDFInfo
- Publication number
- WO1998031150A1 WO1998031150A1 PCT/IL1998/000009 IL9800009W WO9831150A1 WO 1998031150 A1 WO1998031150 A1 WO 1998031150A1 IL 9800009 W IL9800009 W IL 9800009W WO 9831150 A1 WO9831150 A1 WO 9831150A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- mini
- biological sample
- microscopes
- incubator
- microscope
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N7/00—Television systems
- H04N7/18—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast
- H04N7/181—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast for receiving images from a plurality of remote sources
Definitions
- the present invention relates to a method for monitoring the development of
- An incubator is a closed
- atmospheric composition can be optimized to promote the growth of the cell cultures.
- mammalian embryos should be incubated under conditions resembling
- photomicroscope is used, it can be focused on successive focal image planes within
- Miyamoto places a solid-state area image sensor array, such as a charge coupled
- CCD CCD array
- CCD arrays are small enough to fit inside commonly used incubators. If positioned
- a CCD array does not need an optical system in
- a display unit such as a video monitor, and also may be digitized
- microscopes including: (i) a microscope objective, and (ii) a solid-state area image
- mini-microscopes on a focal image plane, at a focal distance from the mini-
- monitoring the development of a biological sample comprising: (a) an incubator; (b) a
- microscopes mounted relative to the transparent holder within the incubator so that,
- mini-microscopes may be focused on a focal image plane within the biological
- the present invention is made possible by a newly available type of camera,
- CCD mini-cameras are manufactured, for example, by Aplitec Ltd. of Holon,
- FIG. 1A is a schematic cross section through a CCD mini-camera 10
- Optical system 14 may be removed and replaced by a
- optical system may be replaced, as shown in Figure IB, by an adapter 22
- Adapter 22 is configured to position
- CCD array 16 immediately in front of objective 24 is focused on CCD array 16. This converts CCD mini-camera 10 into a CCD mini-photomicroscope 20.
- CCD mini-camera 10 immediately in front of objective 24 is focused on CCD array 16.
- optical axis of mini-photomicroscope 20 is designated by the reference numeral 26.
- mini-photomicroscope 20 is shown configured with CCD array 16, the
- scope of the present invention includes mini-photomicroscopes configured with any
- Mini-photomicroscope 20 is small enough so that several mini-
- photomicroscopes 20 may be mounted inside a conventional incubator.
- mini-photomicroscopes 20 may be mounted within an
- FIG. 1 A (prior art) is a schematic cross-section of a CCD mini-camera.
- FIG. IB is a schematic cross-section of the mini-camera of FIG. 1A
- FIG. 2 is a schematic diagram of an incubator system provided with two of the
- mini-photomicroscopes of FIG. IB for monitoring biological samples in Petri dishes.
- FIG. 3 is a schematic diagram of two of the mini-photomicroscopes of FIG.
- FIG. 4 is an alternative configuration of the mini-photomicroscope of FIG. IB. DESCRIPTION OF THE PREFERRED EMBODIMENTS
- the present invention is of a method and apparatus for monitoring the
- Figure 2 is a schematic diagram of one
- the apparatus comprises an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator 30, within an incubator
- Photomicroscopes 20, mounted on mountings 40 include step motors
- Figure 2 shows only two mini-photomicroscopes 20; the
- the apparatus includes at least four mini-photomicroscopes.
- control system 60 which is connected to light source 50 and
- stepping motors 44 by suitable electrical connections 62, such as coaxial cables.
- the signals may be
- photomicroscopes 20 are recorded together on the same recording medium, for
- control system 60 directs stepping motors to move mini-
- photomicroscopes 20 up and down continuously, over an appropriate range of
- time lapse 3D images of the biological samples may
- the means for controlling the environmental parameters such as temperature, temperature, and temperature
- the apparatus of Figure 2 may be used to conveniently monitor the
- incubator 30 is
- Light source 50 is turned on
- mini-photomicroscopes 20 area image sensors of mini-photomicroscopes 20. While light source 50 is on, mini-
- photomicroscopes 20 may be moved up and down to focus, from different focal
- source 50 may be turned on at regular intervals of on the order of several hours,
- photomicroscopes 20 are long integration time CCD arrays, because of their ability to
- optic fiber cables 52 may be dispensed with, and a light source 50 of sufficiently
- Figure 3 shows an alternative configuration of mini-photomicroscopes 20
- the transparent holder of the biological sample is
- photomicroscopes 20 are focused on the same point in the biological sample, the
- intersection point 27 of optical axes 26 of mini-photomicroscopes 20 As in the
- fiber optic cables 52 are provided for shining light into
- capillary tube 36 from the sides opposite mini-photomicroscopes 20.
- mini-photomicroscopes 20 For simplicity,
- scope of the present invention also includes capillary tubes of any suitable polygonal
- mini-photomicroscopes 20 are mounted
- Figure 4 is a schematic diagram of an alternative embodiment of the mini-
- a mirror 70 intersected by optical axis 26, and a port 74 through
Landscapes
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Multimedia (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Thermal Sciences (AREA)
- Computer Vision & Pattern Recognition (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Signal Processing (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Microscoopes, Condenser (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU54939/98A AU5493998A (en) | 1997-01-09 | 1998-01-07 | Method and apparatus for monitoring a biological sample |
DE69831933T DE69831933T2 (en) | 1997-01-09 | 1998-01-07 | DEVICE AND METHOD FOR MONITORING A BIOLOGICAL SAMPLE |
EP98900340A EP0951778B1 (en) | 1997-01-09 | 1998-01-07 | Method and apparatus for monitoring a biological sample |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/781,015 US6166761A (en) | 1997-01-09 | 1997-01-09 | Method and apparatus for monitoring a biological sample |
US08/781,015 | 1997-01-09 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1998031150A1 true WO1998031150A1 (en) | 1998-07-16 |
Family
ID=25121400
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IL1998/000009 WO1998031150A1 (en) | 1997-01-09 | 1998-01-07 | Method and apparatus for monitoring a biological sample |
Country Status (5)
Country | Link |
---|---|
US (1) | US6166761A (en) |
EP (1) | EP0951778B1 (en) |
AU (1) | AU5493998A (en) |
DE (1) | DE69831933T2 (en) |
WO (1) | WO1998031150A1 (en) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000043820A1 (en) * | 1999-01-22 | 2000-07-27 | Q3Dm, Llc | Automatic on-the-fly focusing for continuous image acquisition in high-resolution microscopy |
FR2820756A1 (en) * | 2001-02-09 | 2002-08-16 | Daniel Attias | INCUBATOR AND INCUBATION PROCESS ENDING THE ORGANIZATION SET TO INCUBATE |
EP1316793A1 (en) * | 2001-12-03 | 2003-06-04 | Christian Leist | Process and apparatus for characterization of a collection of cells |
US6815687B1 (en) * | 1999-04-16 | 2004-11-09 | The Regents Of The University Of Michigan | Method and system for high-speed, 3D imaging of optically-invisible radiation |
WO2006005811A1 (en) * | 2004-07-09 | 2006-01-19 | Chip-Man Technologies Oy | A substructure for cultivating cells and its use |
WO2006005810A1 (en) * | 2004-07-09 | 2006-01-19 | Chip-Man Technologies Oy | An apparatus for imaging cells |
EP2028264A1 (en) * | 2006-06-15 | 2009-02-25 | Nikon Corporation | Cell incubator |
EP2088460A1 (en) * | 2006-12-01 | 2009-08-12 | Nikon Corporation | Image processing device, image processing program, and observation system |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2003207980A1 (en) * | 2002-06-27 | 2004-01-19 | I.M.T. Interface Multigrad Technology Ltd. | Changing the temperature of a liquid sample and a receptacle useful therefor |
US7331186B2 (en) * | 2002-06-27 | 2008-02-19 | I.M.T. Interface Multigrad Technology Ltd | Changing the temperature of a liquid sample and a receptacle useful therefor |
GB2423151B (en) * | 2003-07-23 | 2007-05-09 | Essen Instr Inc | Examination systems for biological samples |
DE102005023855A1 (en) * | 2004-05-26 | 2006-01-26 | Olympus Corporation | Culture microscope and computer program for controlling the culture microscope |
WO2011025736A1 (en) | 2009-08-22 | 2011-03-03 | The Board Of Trustees Of The Leland Stanford Junior University | Imaging and evaluating embryos, oocytes, and stem cells |
CA2812776C (en) | 2010-09-27 | 2022-05-17 | Auxogyn, Inc. | Apparatus, method, and system for the automated imaging and evaluation of embryos, oocytes, and stem cells |
JP2014507664A (en) | 2011-02-23 | 2014-03-27 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | Method for detecting aneuploidy in human embryos |
KR101384843B1 (en) * | 2012-09-07 | 2014-05-07 | 주식회사 나노엔텍 | microscope and controlling method thereof |
CA2900741C (en) | 2013-02-01 | 2022-01-18 | Progyny, Inc. | Abnormal syngamy phenotypes observed with time lapse imaging for early identification of embryos with lower developmental potential |
CN109337804B (en) * | 2013-07-05 | 2022-07-08 | 爱特医疗有限公司 | A equipment for monitoring biomaterial is developed |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4902132A (en) * | 1988-06-13 | 1990-02-20 | Hipple Cancer Research Corporation | Automated capillary scanning system |
US5109276A (en) * | 1988-05-27 | 1992-04-28 | The University Of Connecticut | Multi-dimensional multi-spectral imaging system |
US5307161A (en) * | 1991-04-12 | 1994-04-26 | Nec Corporation | Biological sample observation system using a solid state imaging device |
US5397709A (en) * | 1993-08-27 | 1995-03-14 | Becton Dickinson And Company | System for detecting bacterial growth in a plurality of culture vials |
US5465114A (en) * | 1992-02-12 | 1995-11-07 | Nec Corporation | Biological sample observation system using a temperature controlled solid state sensor |
US5541081A (en) * | 1994-03-22 | 1996-07-30 | President And Fellows Of Harvard College | Process for assessing oocyte and embryo quality |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0611547A1 (en) * | 1993-02-03 | 1994-08-24 | J.D. Möller Optische Werke GmbH | Operation microscope |
WO1994018592A1 (en) * | 1993-02-08 | 1994-08-18 | Optiscan Pty Ltd Acn 060 658 754 | Confocal microscope |
-
1997
- 1997-01-09 US US08/781,015 patent/US6166761A/en not_active Expired - Fee Related
-
1998
- 1998-01-07 WO PCT/IL1998/000009 patent/WO1998031150A1/en active IP Right Grant
- 1998-01-07 AU AU54939/98A patent/AU5493998A/en not_active Abandoned
- 1998-01-07 EP EP98900340A patent/EP0951778B1/en not_active Expired - Lifetime
- 1998-01-07 DE DE69831933T patent/DE69831933T2/en not_active Expired - Fee Related
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5109276A (en) * | 1988-05-27 | 1992-04-28 | The University Of Connecticut | Multi-dimensional multi-spectral imaging system |
US4902132A (en) * | 1988-06-13 | 1990-02-20 | Hipple Cancer Research Corporation | Automated capillary scanning system |
US5307161A (en) * | 1991-04-12 | 1994-04-26 | Nec Corporation | Biological sample observation system using a solid state imaging device |
US5465114A (en) * | 1992-02-12 | 1995-11-07 | Nec Corporation | Biological sample observation system using a temperature controlled solid state sensor |
US5397709A (en) * | 1993-08-27 | 1995-03-14 | Becton Dickinson And Company | System for detecting bacterial growth in a plurality of culture vials |
US5541081A (en) * | 1994-03-22 | 1996-07-30 | President And Fellows Of Harvard College | Process for assessing oocyte and embryo quality |
Non-Patent Citations (1)
Title |
---|
See also references of EP0951778A4 * |
Cited By (17)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000043820A1 (en) * | 1999-01-22 | 2000-07-27 | Q3Dm, Llc | Automatic on-the-fly focusing for continuous image acquisition in high-resolution microscopy |
US6640014B1 (en) | 1999-01-22 | 2003-10-28 | Jeffrey H. Price | Automatic on-the-fly focusing for continuous image acquisition in high-resolution microscopy |
US6815687B1 (en) * | 1999-04-16 | 2004-11-09 | The Regents Of The University Of Michigan | Method and system for high-speed, 3D imaging of optically-invisible radiation |
FR2820756A1 (en) * | 2001-02-09 | 2002-08-16 | Daniel Attias | INCUBATOR AND INCUBATION PROCESS ENDING THE ORGANIZATION SET TO INCUBATE |
WO2002064728A2 (en) * | 2001-02-09 | 2002-08-22 | Daniel Attias | Incubator and incubation method monitoring the organism to be incubated |
WO2002064728A3 (en) * | 2001-02-09 | 2003-11-06 | Daniel Attias | Incubator and incubation method monitoring the organism to be incubated |
EP1316793A1 (en) * | 2001-12-03 | 2003-06-04 | Christian Leist | Process and apparatus for characterization of a collection of cells |
WO2006005810A1 (en) * | 2004-07-09 | 2006-01-19 | Chip-Man Technologies Oy | An apparatus for imaging cells |
WO2006005811A1 (en) * | 2004-07-09 | 2006-01-19 | Chip-Man Technologies Oy | A substructure for cultivating cells and its use |
EP1771544A1 (en) * | 2004-07-09 | 2007-04-11 | Chip-Man Technologies Oy | An apparatus for imaging cells |
US7885000B2 (en) | 2004-07-09 | 2011-02-08 | Chip-Man Technologies Oy | Apparatus for imaging cells |
EP1771544A4 (en) * | 2004-07-09 | 2011-10-05 | Chip Man Technologies Oy | An apparatus for imaging cells |
EP2028264A1 (en) * | 2006-06-15 | 2009-02-25 | Nikon Corporation | Cell incubator |
EP2028264A4 (en) * | 2006-06-15 | 2012-10-24 | Nikon Corp | Cell incubator |
EP2088460A1 (en) * | 2006-12-01 | 2009-08-12 | Nikon Corporation | Image processing device, image processing program, and observation system |
EP2088460A4 (en) * | 2006-12-01 | 2011-12-21 | Nikon Corp | Image processing device, image processing program, and observation system |
US8150137B2 (en) | 2006-12-01 | 2012-04-03 | Nikon Corporation | Image processing device, image processing program, and observation system |
Also Published As
Publication number | Publication date |
---|---|
EP0951778B1 (en) | 2005-10-19 |
DE69831933D1 (en) | 2006-03-02 |
EP0951778A4 (en) | 2000-04-26 |
AU5493998A (en) | 1998-08-03 |
DE69831933T2 (en) | 2006-07-27 |
US6166761A (en) | 2000-12-26 |
EP0951778A1 (en) | 1999-10-27 |
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