WO1998030246A1

WO1998030246A1 - Composition comprising non-radioactive metal ion complexes with phosphonate compounds for the palliation of pain associated with diseases of the bone and bone joints

Info

Publication number: WO1998030246A1
Application number: PCT/GB1998/000037
Authority: WO
Inventors: Wei Jia
Original assignee: Mitreoak Limited
Priority date: 1997-01-07
Filing date: 1998-01-07
Publication date: 1998-07-16
Also published as: EP0952854A2; AU5491398A; US5902825A

Abstract

A therapeutic composition and method of using the same are provided for the palliation or relief of pain in patients having diseases which affect the bone and bone joints including metastatic bone cancer, arthritis, and other inflammatory arthropathies. The therapeutic composition comprises as the active agent a complex formed of non-radioactive metal ions and organic phosphonic acid ligands, or pharmaceutically acceptable salts thereof.

Description

COMPOSITION COMPRISING NON-RADIOACTIVE METAL ION COMPLEXES WITH PHOSPHONATE COMPOUNDS FOR THE PALLIATION OF PAIN ASSOCIATED WITH DISEASES OF THE BONE AND BONE JOINTS

FIELD OF THE INVENTION The present invention relates generally to the field of therapeutic pharmaceuticals, and is more specifically directed to a composition and method of using the same for the palliation of pain in patients having diseases affecting the bone and bone joints including metastatic bone cancer, rheumatic diseases such as rheumatoid arthritis, osteoarthritis and other inflammatory arthropathies .

BACKGROUND OF THE INVENTION Various forms and types of disease are known to negatively affect and damage the bones and bone joints of humans and animals. Typically these diseases cause severe and often chronic pain which increases over time eventually progressing to result in loss of function and/or destruction of the joints of the body. The source of this pain and joint damage varies depending upon the disease.

For instance, osteoarthritis is a common degenerative joint disorder which normally affects older persons. Osteoarthritis is marked by inflammation of the joint 5 causing swelling, pain and stiffness reducing the mobility and activity of the patient. These symptoms increase in intensity and regularity with advancing age. The constant inflammation can eventually lead to complete or partial loss of function and damage to the larger weight-bearing joints of the body.

The rheumatic diseases, particularly rheumatoid arthritis, juvenile rheumatoid arthritis and psoriatic arthritis are also marked by chronic inflammation primarily of the synovial tissue leading to pannus formation and eventual destruction of the articular cartilage. In addition to inflammation problems, these diseases also cause the release of various enzymes including collagenase and lysosomal enzymes in the affected area. These enzymes act to indisciminately destroy extra-cellular collagen fibers and ultimately destroy the connective tissue surrounding the joints.

Another relatively common disease known to affect the bone is metastatic bone cancer which often times accompanies primary cancers in other tissues, notably the lung, breast, and prostate. This form of cancer causes abnormal cell activity in bone, resulting in severe pain and consequent immobility, anorexia, and the need for long- term narcotic analgesia. More specially, this disease is often associated with overactive osteoblasts (cells which are normally associated with new bone growth) in the bone which cause excessive bone growth in the area of the cancer. Alternatively, the disease may cause abnormally high osteoclastic activity (osteoclasts are cells normally associated with bone resorption) in bone resulting in the destruction of the bone material. Those individuals having excessive osteoclastic activity may also suffer from a condition known as hypercalcaemia caused by excessive levels of calcium ions in the blood resulting from the bone being dissolved or destroyed at an increased rate. Osteoarthritis, the rheumatic diseases, metastatic bone cancer and other less common forms of inflammatory arthropathies affect great numbers of people in the USA and elsewhere. The cost of treatment and care combined with loss of work time and productivity is incalculable. For instance, it is estimated that one to three percent of the population in the United States is afflicted with rheumatoid arthritis with more than half of these patients suffering inflammation and eventual destruction of the hand and knee joints. With an increasing survival time in the population, these diseases constitute one of the greatest medical, social, and economic problems existing today.

Successful treatment of these diseases generally focuses on pain relief, reduction of inflammation, and preservation of the remaining functional capacity of joints and the adjoining muscles. While complete resolution of the pathological process (e.g. a cure) has not been found, successful early treatment particularly in the case of rheumatoid and osteoarthritis may avert the destructive, deforming phase of the disease. Presently, the primary method of treating the rheumatoid diseases and arthritis is by drug therapy using anti-inflammatory compounds directed at blocking or reducing synovial inflammation thereby improving function and analgesics directed to reducing pain. Aspirin and other salicylate compounds are frequently used in treatment to interrupt ampli ication of the inflammatory process and temporarily relieve the pain. Other drug compounds used for these purposes include phenylpropionic acid derivatives such as Ibuprofen and Naproxin, Sulindac, phenyl butazone, corticosteroids, antimalarials such as chloroquine and hydroxychloroquine sulfate, and fenemates. For a thorough review of various drugs utilized in treating rheumatic diseases, reference is made to J. Hosp. Pharm. , 36:622 (May 1979) . While these known drug therapies have utility, there are drawbacks to their use. For instance, it may take up to six months of consistent use of some of these medications in order for the product to have effect in relieving the patient's pain. Consequently if the product is not effective for that particular patient, it could take up to six months before the physician is able to accurately make that assessment. Many of these drugs also cause serious side effects in certain patients, and therefore the patient should be carefully monitored to assure that these side effects are not unduly threatening. Most of these drugs bring only temporary relief to the patient and therefore must be taken consistently on a daily or weekly basis for continued relief. As the disease progresses, the amount of medicament needed to alleviate the pain may increase to relatively high daily doses. The potential for adverse side effects consequently increases with higher dosage amounts . To further complicate matters , many of these patients suffer from other conditions which require them to take a variety of different medications, increasing the risk of negative side effects and interaction between drugs .

Some treatments not only reduce the amount of inflammation and pain, but actually slow the progression of the disease or joint destruction. For instance, gold salts such as gold sodium thiomalate, aurothioglucose, and other sources of auric and aurous ions are said to produce a favorable response in about 75 percent of patients with actual disease remission in 20-25 percent. One major drawback to the use of gold therapy is that the gold is distributed via the blood system not only to the diseased joint, but to a number of tissues in the body including the liver, skin, bone, bone marrow, eyes and reticulo- endothelial system. The gold often causes serious adverse reactions in these tissues including dermatitis, nephrotoxicity, blood dyscrasias and ocular toxicity. A certain amount of the gold also binds with humoral or cellular components such as serum albumin making the bound gold unavailable for purposes of treatment.

Since only a percentage of the total amount of gold administered is actually delivered to the diseased site, significantly high doses of the compound are required for effective treatment. Yet, the risk of adverse reactions is only exacerbated and more pronounced, in certain circumstances becoming life threatening, by virtue of the large dosage amounts administered. It is known to reduce the toxic effects of gold therapy by use of a selenium- containing compound. (U.S. Patent No. 4,680,286 to Stockel, et al . ) . However, the amount of gold required for treatment and delivered to the various organs of the body is not reduced using this detoxification method, the selenium is merely provided to expedite elimination of the gold from those organs which can be most severely negatively affected such as the renal system and liver.

A relatively new method of treating rheumatoid arthritis which is also believed to actually slow the progression of the disease is the use of D-penicillamine, a natural metabolite of penicillin. Like many of the other drugs used in the past, penicillamine has drawbacks because it is relatively slow-acting requiring 8-12 weeks of use before it can be determined whether the patient is responding. Penicillamine can also cause several adverse effects and can be toxic in some patients.

Where the inflammation is present in larger joints such as the knee for example, an alternative to drug therapy is the surgical incision of the inflamed synovium using a procedure known as surgical synovectomy. In this procedure the inflamed synovium and pannus formation are surgically removed which tends to relieve the pain and in many cases proves to arrest the disease. In order to avoid the risks of surgery it is also known to destroy the diseased synovium by a procedure known as radiation synovectomy. Radiation synovectomy consist of injecting a radionuclide directly into the articular region affected whereby the radiation kills the diseased tissue to abate the inflamed synovium. Known methods of radiation synovectomy include intra-articular injection of ¹¹Sn hydroxide in a carrier (See U.S. Patent No. 4,906,450 to Lieberman, et al.) and ¹⁵³Sm labeled particulate hydroxyapatite . Radionuclides have also been delivered to the affected area by the use of bone seeking carriers or agents. For instance, the use of β-emitting radionuclides ^ιa6Re and ¹⁵³Sm complexed with phosphonates have been suggested for relieving pain in patients with metastatic bone cancer. (See, Coordination Compounds in Nuclear Medicine, Chem . Rev. 1993, 93, 1137-1156, 1148). Phosphonates were initially shown to have a high affinity for sites of actively growing bone when used in skeletal or bone imaging. Di-phosphonate products have also been used for the treatment of various diseases associated with bone joint pain and destruction. For example, 3-amino-l- hydroxypropylidine 1-di-phosphonate commercially available as Pamidronate from Ciba Geigy is known to reduce the pain of abnormal bone turnover known to exist in Paget's Disease (a disease with the symptoms of marked pain and stiffness of bone and joints). Another di-phosphonate product known as Clodronate (di-chloromethane di-phosphonate) and commercially available from Boehringer has been found to reduce pain and damage in patients having extensive destruction of the lumbar spine. The di-phosphonates have also been used as resorption inhibitors for the treatment of malignant hypercalcaemia associated with overactive osteoclasts, (See .Bone Metastases, R.D. Rubins and I. Fogelman, Springer-Verlag London Ltd. 1991). In a recent study of radionuclide complexes used for radiotherapy in bone tumors, complexes of ^117mSn with the ligands of pyrophosphate, phosphonate, and diethylene- triaminepentaacetic acid (DTPA) were prepared. The investigators reported that the ^117lBSn-DTPA complex was best suited for radiotherapy and that the tin compound itself acted as a bone-localizing agent having a tendency for high bone uptake. See, e.g. Int . J. Nucl . Med. Biol . , Vol . 12 , No . 3 , pp 167-174 (1985) ; see also. Radiology, Vol . 186, No . 1 pp . 279-283 (1993) . Thus, the two methods that have primarily been suggested for radiotherapy are articular injection whereby the material is directly injected into the joint for treatment, or i.v. injection whereby the radionuclide complexed with a ligand such as phosphonate is injected intravenously and rapidly localized to the skeleton. While these methods of application attempt to distribute the radionuclide only to the affected area, studies have indicated that activity is leaked or distributed to other parts of the body even when using these techniques. The major drawbacks of these radiotherapeutic agents are the relatively low bone lesion uptake and undesirably high radiation dose to normal tissues. The other problem involved in the preparation of these radiotherapeutic agents is the lack of availability of the radionuclides. Radionuclides are produced from a nuclear reactor or an accelerator. Currently there are very few of the radionuclide production facilities operating in the world, which have seriously limited the availability of the medical radionuclides. Furthermore, many radionuclides are either difficult to produce, such as ^117BSn, which can only be produced from a high flux reactor, or too short-lived to be shipped nationwide, such as Sn (27 hours). As a result, there are very few radiodiagnostic and radiotherapeutic bone agents that have been approved by the United States Food and Drug Administration.

While the rheumatoid diseases, osteoarthritis, metastatic bone cancer and other crippling arthropathies differ somewhat as to source and symptoms, there are certain factors common to each. Firstly, the disease causes chronic or severe pain which significantly impairs the quality of life for the patients. Secondly, the disease, when advanced, causes bone destruction and/or abnormal bone growth. Thirdly, while various forms of drug therapy have been developed for treating these conditions, none have proven successful without continued dosing and/or unwanted side effects. In ddition, the drug may be distributed throughout many parts of the body causing toxic side effects in the skin, the liver, the urinary tract and other vital organs. Fourthly, while various forms of radiotreatment have been developed to destroy diseased tissue and/or reduce pain, these treatments involve the use of potentially harmful radioactive materials in the body, are relatively expensive and not easily adapted for convenient use in many doctor's offices or clinics. Thus, there remains a need in the art to develop new methods of treatment to reduce the pain experienced by these patients, and to reduce or prevent joint destruction with minimal negative side effects to the patient.

An object of the present invention is to provide a composition, method and use for the palliation of pain in patients with metastatic bone cancer, arthritis, or other inflammatory arthorpathies which does not require the use of a radionuclide or other radioactive material.

Another object of the present invention is to provide a composition, method and use for the palliation of pain in patients with metastatic bone cancer, arthritis, or other inflammatory arthropathies wherein the active therapeutic agent is administered to the patient in relatively low dosage amounts . A further object of the present invention is to provide a composition, method and use for the palliation of pain in patients with metastatic bone cancer, arthritis, or other inflammatory arthropathies which not only relieves the pain associated with the disease, but also inhibits and/or prevents further destruction of the diseased tissue. Another object of the present invention is to provide a composition, method and use for the palliation of pain in patients with metastatic bone cancer, arthritis, or other inflammatory arthropathies wherein the active therapeutic agent is selectively delivered to the bone and bone joints, and specifically to those areas of tissue affected by the disease.

A further object of the present invention is to provide a composition, method and use for the palliation of pain in patients with metastatic bone cancer, arthritis, or other inflammatory arthropathies wherein single treatments are effective for relatively long term pain relief without the need for daily and/or weekly administration.

A further object of the present invention is to provide a composition, method and use for the palliation of pain in patients with metastatic bone cancer, arthritis, or other inflammatory arthropathies wherein pain relief and prevention of further tissue damage can be achieved through the administration of small dosage amounts which are effective for extended periods of time.

A further object of the present invention is to provide a composition, method and use for the palliation of pain in patients with metastatic bone cancer, arthritis, or other inflammatory arthropathies wherein the active therapeutic agent is selectively delivered to the diseased areas providing immediate pain relief as well as relatively long term prevention of tissue destruction by blocking the inflammatory process and/or inhibiting osteoblastic or osteoclastic overactivity.

SUMMARY OF THE INVENTION

The invention provides, in one of its aspects, a composition for use in therapy e.g. for the palliation of pain in patients having diseases of the bone and/or bone joints, said composition comprising a complex of a non- radioactive metal ion and phosphonate compounds selected from phosphonic acid and pharmaceutically acceptable salts thereof.

This invention also comprehends method and use aspects involving a composition according to the invention.

The invention provides, in another of its aspects, a method for the palliation of pain associated with a disease of the bone and/or bone joints, said method comprising depositing a precipitate layer of a metal ion-phosphate complex on the surface of the bone or bone joint affected by the disease.

The invention also provides, in another of its aspects, use of a metal ion-phosphate complex for the manufacture of a medicament for the palliation of pain associated with a disease of the bone and/or bone joints by depositing a precipitate layer of the metal ion-phosphate complex on the surface of the bone or bone joint affected by the disease.

The metal ion is for example selected from metal ions capable of forming a complex with said phosphonate compounds and metal ions capable of forming water insoluble complexes with phosphate ions.

The invention further provides, in another of its aspects, a composition, method or use according to the invention wherein tissue destruction is inhibited and/or prevented, in addition to the palliation of pain.

In embodiments of the invention a therapeutic composition and method of using the same are employed for the palliation or relief of pain in patients having diseases which affect the bone and bone joints including metastatic bone cancer, arthritis, and other inflammatory arthropathies. The therapeutic composition comprises as the active agent a complex formed of metal ions and organic phosphonic acid ligands, or pharmaceutically acceptable salts thereof, wherein the metal ions used may be those which carry a relatively high formal charge of +3 or greater and tend to form insoluble phosphate complexes .

When administered into the body, the metal ion-phosphonate complex is selectively distributed to the skeletal system and more specifically to those areas affected by disease such as in areas of synovial inflammation, bone resorption, or osteoblastic overactivity .

The selective distribution is believed attributable to the high affinity that phosphonates have for the hydroxyapatite surface of the bone with enhanced deposition in regenerating bone. The phosphonates are known to be resistant against attack by biological enzymes such as phosphatase. Thus, the highly charged metal ions complexed with the phosphonates are selectively transported to the area of the disease and are prevented from binding with other humoral or cellular components due to the chelation of the phosphanate ligands .

While not wishing to be bound by theory, it is believed that once the complex reaches the diseased tissue, phosphate ions present on the surface of the bone replace the phosphonate ligands and form a very stable complex with the metal ion. The metal phosphate complexes form a precipitate layer on the bone surface. The freed phosphonates then interact with components in the bone lesion sites similar to how the known di-phosphonate products of Edidronate, Clodronate and Pamidronate behave to temporarily inhibit abnormal cellular activity and relieve pain.

While the phosphonates are believed to provide only temporary relief before degradation, the metal ion phosphate precipitate layer formed on the bone surface is extremely stable and not subject to degradation. This relatively insoluble precipitate layer is believed to interfere or block bone resorption caused by enzymes or abnormal osteoclastic cell activity, osteoblastic overactivity, as well as inflammation within the joint region and on the bone surface. The inorganic precipitate of metal ion and phosphate is therefore believed to act as a palliative agent and also as an agent for preventing further destruction of the bone. Since the metal ion- phosphate complex is extremely stable and secured to the bone surface as a precipitate, it will remain for a long period of time providing therapeutic relief for extended periods.

DETAILED DESCRIPTION OF THE INVENTION

The description which follows is by way of example of the invention only and not by way of limitation thereof.

A therapeutic composition embodying the present invention comprises an active agent complex formed of a metal ion and an organic phosphonic acid ligand, or a pharmaceutically acceptable salt thereof, wherein the metal ion is not radioactive and has a relatively high charge of +3 or greater. It is believed that in vivo at the bone lesion sites, the phosphonates are bound to the surface of hydroxyapatite through calcium metal in the bone matrix. The metal ion forms a relatively insoluble stable complex with phosphate ions present on the bone surface, which forms a precipitate layer on the surface of the bone. This layer is believed to block or interfere with bone resorption, osteoblastic activity and inflammation to relieve pain and joint destruction long term.

The metal ion to be used in accordance with this invention is capable of forming stable complexes with organic phosphonates and of forming stable complexes with phosphate on the bone surface. Metal ions meeting these requirements form basic oxides and carry a relatively high formal charge of +3 or greater. Particularly suitable metal ions tend to form insoluble phosphates or hydroxides and thus are insoluble and immobile in vivo meaning that they exist as a precipitate on the bone surface for extended periods of time. Specific metal ions preferred for purposes of this invention are selected from Gallium

III (Ga⁺³), Tin IV (Sn⁺⁴), Indium III (In⁺³), and all Lanthanides in the series including Samarium III (SM⁺³), and

Cerium III (Ce⁺³) . Gallium III (Ga⁺³) and Tin IV (Sn⁺⁴) are preferred insofar as both of these metal ions have been used and approved for various uses in the body. For example, tin has been used in a number of different radiopharmaceuticals such as for skeletal imaging. A gallium nitrate product has been used in the treatment of tumor induced hypercalcaemia.

The metal ion is complexed with an excess amount of organic phosphonic acids, or the pharmaceutically acceptable salts thereof . Particularly suitable organic phosphonic acids for purposes of the present invention are selected from organic di-phosphonic acids, triphosphonic acids, tetra-phosphonic acids and mixtures thereof. Suitable di-phosphonic acids include ethylenehydroxy- diphosphonic acid (EHDP), methylenediphosphonic acid (MDP), and aminoethyl-diphosphonic acid (ADEP) . Suitable triphosphonic acids include nitrilotri-(methylene)- phosphonic acid (NTP) and aminotrismethylene-phosphonic acid (AMP) . Tetra-phosphonic acids suitable for purposes of this invention include ethylenediaminetetramethylene- phosphonic acid (EDTMP) , nitrilotri-methylene phosphonic acid (NTMP), tetraazacyclododecanetetramethylene phosphonic acid (DOTMP), and diethylene-triaminepentamethylene phosphonic acid (DTPMP) . The preferred phosphonic acids include methylenediphosphonic acid (MDP) , ethylenehydroxydiphosphonic acid (EHDP) , and ethylenediaminetetramethylene-phosphonic acid (EDTMP) , and tetraazacyclododecanetetramethylene phosphonic acid (DOTMP) having the formulas: HO

MDP EHDP

EDTMP

Most of the phosphonate ligands are commercially available in the acid form or may be readily prepared by methods known to those skilled in the art. Depending upon the metal ion being complexed, the phosphonates may alternatively be converted or obtained as a base addition salt in order to provide a pH environment which is more conducive to formation of the complex with the ions . Any pharmaceutically acceptable cation may be used to prepare these salts provided the cation is chosen to retain the biological effectiveness and properties of the corresponding free acid. Suitable cations for binding with the acid ligands may be derived from inorganic bases including sodium, potassium, lithium, ammonium, calcium, and magnesium, or from organic bases including primary, secondary, and tertiary amines. The preferred bases comprise sodium hydroxide, potassium hydroxide, potassium carbonate, sodium carbonate and magnesium carbonate.

While it is believed that any number of different metal ion phosphonate complexes meeting the above criteria can be used for purposes of the present invention, the preferred complexes are selected from Sn(IV)-MDP, Sn(IV)-

EDTMP, Sn( IV) DOTMP, and Ga( III) -EHDP.

Compositions comprising these complexes as the active agent may be used in treating metastatic bone cancer, rheumatoid diseases, osteoarthritis and other forms of inflammatory arthropathies by administering a therapeutically effective amount of the complex to the human or animal in need. Administration of the active compounds can be affected via any medically acceptable mode of administration for agents which control inflammation and associated pain. These methods include, but are not limited to, oral, parenteral including subcutaneous, sublingual, intravenous and intra-articular injection and other known methods of systemic administration.

In a preferred method of making the complex, the phosphonic acid, or pharmaceutically acceptable salt thereof, is fully dissolved in a solvent selected from water, aqueous alcohols, glycols, phosphonate esters or carbonate esters to provide a dissolved phosphonate solution. Optionally a base may be added and/or the solution may be heated to fully dissolve the phosphonates in solution. The concentration of phosphonates in solution will vary depending upon the amount of metal ion intended to be complexed and the solubility of the phosphonate. In general, it is preferred that the phosphonate concentration in the dissolved solution range from 0.5 to 15 percent by weight and preferably from 2 to 7 percent by weight.

To ensure that all of the metal ion present in the composition is complexed, it is preferable to use an excess amount of ligand in preparing the complex. In this manner the phosphonate ligand will completely surround or encompass the metal ions to prevent the metal ion from being absorbed into the soft tissue and to prevent the metal ion from bonding with other humoral or cellular components during transport to the affected areas. The metal ion, normally in the form of a metal salt, is therefore added to at least a 2 to 50 fold molar excess of the dissolved phosphonate, preferably to a 3 to 20 fold molar excess of the dissolved phosphonate, to form the complex. Once the complex is formed, the resulting complex solution is then adjusted to a biologically acceptable pH ranging from a pH of 4 to 8, and preferably a pH of 6 to 7 by the addition of a base such as sodium hydroxide. The pH adjusted complex solution may then be further processed to provide the complex in a form suitable for administration. The complex may be incorporated into any pharmaceutically acceptable dosage forms in conventional manner including tablets, suppositories, pills, capsules, powders, liquids, suspensions, emulsions, and the like. The dosage form will generally include a pharmaceutically acceptable excipient, the active complex may additionally include other medicinal agents, pharmaceutical agents, carriers , adjuvants or stabilizers which enhance the therapeutic effectiveness of the composition and/or facilitate easier administration of the composition.

In preparing solid dosage forms, the complex may be incorporated with non-toxic solid carriers such as pharmaceutical grades of mannitol, Sorbitol, lactose, starch, magnesium stearate, sodium saccharin, the polyalkylene glycols, talcum, cellulose glucose, sucrose, and magnesium carbonate, cyclodextrin and the like; binding agents such as cellulose, methylcellulose, hydroxy- propylcellulose, hydroxy-propylmethylcellulose, polyvinyl pyrollidone, gelatine, gum arabic, polyethylene glycol, white sugar, starch and the like; disintegrators such as starch, carboxy-methylcellulose, calcium salts of carboxy- methylcellulose and the like; lubricants such as talc and the like; and/or preservatives such as sodium benzoate, sodium bisulfite and the like. Liquid dispersions for oral administration such as syrups, emulsions and suspensions will generally include the complex in a carrier such as saccharose or saccharose with glycerine, mannitol, sorbitol, a natural gum, agar, sodium alginate, pectin, methylcellulose, carboxy- methylcellulose, and/or polyvinyl alcohol.

In preparing liquid solutions for intravenous or intra-articular injections, the complex is preferably sterile filtered into an injection bottle having a suitable carrier such as sterile water or preferably sterile aqueous isotonic saline solutions.

If desired, the therapeutic composition to be administered may also contain minor amounts of nontoxic auxiliary substrates such as wetting or emulsifying agents, pH buffering agents and the like. Typical examples of such auxiliary agents are sodium acetate, sorbitan monolaurate, triethanolamine sodium acetate, triethanolamine oleate, etc.

The therapeutic composition will, in any event, contain a quantity of the active agent complex in an amount effective to alleviate the symptoms. The therapeutic regimen for the different clinical syndromes must be adapted to the type of pathology taking into account the route of administration, the form in which the complex is administered and the age, weight and conditions of the subject involved. Taking into consideration the foregoing, doses of the active complex will broadly range from about 0.2 - 5 mg complex per kg of body weight. Of course, these dosage regimens may be adjusted to provide the optimal theraputic response. The following examples are for purposes of illustrating a suitable method of making complexes embodying this invention and are not intended to be limiting as to the method employed for making the complex or the components used for making the complex. EXAMPLE I

A Sn(IV)-MDP complex was formed by mixing 25mg of SnCl₂ * H₂0 (containing 13.15 mg of Sn) with 97 mg of Na-MDP in a reaction vial containing 5 ml of distilled water. The pH of the resulting solution was adjusted to a pH of 9 to 10 by addition of NaOH. The resulting solution was heated to between 80° - 90° C for 10 minutes. About 1 ml of 30% H₂0₂ was added after cooling and the sample was reheated in a boiling water bath for 5 minutes. The pH of the solution was then adjusted to 7-8 by addition of HC1. The volume of solution was adjusted to 10 ml using physiological saline. The molar ratio of metal to ligand (Sn:MDP) in the complex is - 1:4.

EXAMPLE II

In the preparation of a Sn(IV) -EDTMP complex, the stannous chloride, SnCl₂ • H₂0, was dissolved in a 1 N HC1 solution and then added to a 3-fold molar excess (with respect to tin) of the sodium salt of EDTMP. While stirring, NaOH was added to the mixture until a clear solution ws obtained. A 1 : 1 molar amount of CaCl₂ ^• 2H₂0 (based on EDTMP) was added and NaOH was added dropwise until the white precipitate disappeared. The preparation was heated at 100 'C for 10 minutes to ensure complexation.

A 2-fold equivalent excess of 30% H₂0₂ was added after cooling and the solution was reheated in a boiling water bath for 5 minutes. The final pH was adjusted to - 8.5 using HC1.

It will be understood that certain features and subcombinations are of utility and may be employed without reference to other features and subcombinations.

Claims

CLAIMS :

1. A composition for use in therapy e.g. for the palliation of pain in patients having diseases of the bone and/or bone joints, said composition comprising a complex of a non-radioactive metal ion and phosphonate compounds selected from phosphonic acid and pharmaceutically acceptable salts thereof.

2. A composition according to claim 1, wherein said metal ion is selected from metal ions capable of forming a complex with said phosphonate compounds and of forming water insoluble complexes with phosphate ions.

3. A composition according to claim 1 or claim 2, wherein said metal ion has a charge of + 3 or greater.

4. A composition according to any one of claims 1, 2 and 3, wherein said metal ion is selected from Indium III (In⁺³), Samarium III (Sm⁺³) and Cerium III (Ce⁺³).

5. A composition according to any one of claims 1, 2 and 3, wherein said metal ion is selected from Gallium III (Ga*³) and Tin IV (Sn⁺⁴).

6. A composition according to any one of the preceding claims, wherein said phosphonic acid is selected from organic di-phosphonic acids, triphosphonic acids, tetra-phosphonic acids, tetraaminophosphonic acids and mixtures thereof.

7. A composition according to claim 6, wherein said di-phosphonic acids are selected from ethylenehydroxydiphosphonic acid (EHDP), methylenediphosphonic acid (MOP), and aminoethyl-diphosphonic acid (ADEP) , and mixtures thereof.

8. A composition according to claim 6, wherein said triphosphonic acids are selected from nitrilotri-mythylene- phosphonic acid (NTP) and aminotrismethylene-phosphonic acid (AMP), and mixtures thereof.

9. A composition according to claim 6, wherein said tetra-phosphonic acids are selected from the group consisting of ethylenediaminetetramethylene-phosphonic acid (EDTMP), nitrilotri-methylene phosphonic acid (NTMP), tetraazacyclo-dodecanetetramethylene phosphonic acid (DOTMP) , diethylene-triaminepentamethylene phosphonic acid (DTPMP) , and mixtures thereof.

10. A composition according to claim 1, wherein said composition is selected from Sn(IV)-MDP, Sn(IV)-EDTMP, Sn(IV) -DOTMP and Ga(III) -EHDP.

11. A composition according to any one of the preceding claims, wherein said metal ion is complexed with an excess amount of said phosphonate compound.

12. A composition according to any one of the preceding claims, for the palliation of pain in patients having a disease selected from metastatic bone cancer, rheumatic disease, rheumatoid arthritis, psoriatic arthritis, osteoarthritic, and other inflammatory arthropathies.

13. A method for the palliation of pain in a patient having a disease of the bone and/or bone joints, said method comprising: administering to said patient a composition according to any one of the preceding claims.

14. A method for the palliation of pain associated with a disease of the bone and/or bone joint, said method comprising depositing a precipitate layer of a metal ion - phosphate complex on the surface of the bone or bone joint affected by the disease.

15. Use of a composition as defined in any one of the claims 1 to 12 for the manufacture of a medicament for the palliation of pain in patients having diseases of the bone and/or bone joints.

16. Use according to claim 15, wherein the disease is selected from metastatic bone cancer, rheumatic disease, osteoarthritic, and other inflammatory arthropathies.

17. Use of a metal ion-phosphate complex for the manufacture of a medicament for the palliation of pain associated with a disease of the bone and/or bone joints by depositing a precipitate layer of the metal ion-phosphate complex on the surface of the bone or bone joint affected by the disease.

18. A composition, method or use according to any one of the preceding claims, wherein tissue destruction is inhibited and/or prevented, in addition to the palliation of pain.