WO1998004718A1 - Fusion polypeptides comprising an ige-binding domain and a hsa component, and their diagnostic and therapeutic uses - Google Patents
Fusion polypeptides comprising an ige-binding domain and a hsa component, and their diagnostic and therapeutic uses Download PDFInfo
- Publication number
- WO1998004718A1 WO1998004718A1 PCT/EP1997/004066 EP9704066W WO9804718A1 WO 1998004718 A1 WO1998004718 A1 WO 1998004718A1 EP 9704066 W EP9704066 W EP 9704066W WO 9804718 A1 WO9804718 A1 WO 9804718A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- ige
- hsa
- polypeptide
- leu
- glu
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/765—Serum albumin, e.g. HSA
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/08—Antiallergic agents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70503—Immunoglobulin superfamily
- C07K14/70535—Fc-receptors, e.g. CD16, CD32, CD64 (CD2314/705F)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Definitions
- the invention relates to fusion polypeptides. It concerns fusion polypeptides comprising an IgE-binding domain and a human serum albumin (HSA) component and salts thereof. It also concerns polynucleotides and physiologically functional equivalent polypeptides which are intermediates in the preparation of such fusion polypeptides; appropriate recombinant expression vectors therefor, corresponding procaryotic and eucaryotic expression systems, and processes for synthesizing the fusion polypeptides.
- HSA human serum albumin
- IgE immunoglobulin E
- IgE is the primary antibody involved in initiation of an immediate allergic response and is a major participant in the maintenance of the late phase response. IgE is synthesized in B lymphocytes and exerts its effects after binding to the high affinity receptor for IgE, i.e. Fc ⁇ RI, which is found on the surface of allergy effector cells such as mast cells, basophils and eosinophils. IgE also exerts inducer functions through binding to its receptor on antigen presenting cells such as Langerhans cells, B cells and onocytes (G.C. Mudde et al.. Allergy 50 f 19951 193- 199).
- Fc ⁇ RI the high affinity receptor for IgE
- IgE also exerts inducer functions through binding to its receptor on antigen presenting cells such as Langerhans cells, B cells and onocytes (G.C. Mudde et al.. Allergy 50 f 19951 193- 199).
- An allergic response or condition is manifested when IgE molecules bind to the surface of allergy effector cells via the IgE receptor, Fc ⁇ RI, and become cross-linked by allergen, thereby effecting signalling to initiate degranulation of cytoplasmic granules in the cells, with accompanying release of mediators of allergy such as histamine, serotonin, prostaglandins and cytokines, and consequent local tissue edema and influx of inflammatory cells.
- mediators of allergy such as histamine, serotonin, prostaglandins and cytokines
- An alternate means of stimulating allergy and associated conditions is the interaction of the IgE receptor, Fc ⁇ RI, with circulating autoantibodies against Fc ⁇ RI.
- Fc ⁇ RI typically exists as a tetramer comprising an ⁇ -, a ⁇ - and two ⁇ -chains (i.e. "subunits"), although on monocytes and Langerhans cells, the ⁇ -subunit is absent.
- Fc ⁇ RI ⁇ The IgE binding site of Fc ⁇ RI has been shown to be contained entirely within its ⁇ -subunit (referred to as Fc ⁇ RI ⁇ ) (J. Hakimi et al., J. Biol. Chem. 265 [1990] 22079- 22081; U. Blank et al., J. Biol Chem. 266 [1991] 2639-2646). Recombinant "knockout" mice genetically deleted for the entire ⁇ -subunit have been found to be unable to mount an allergic response to allergen challenge (D. Dombrowicz et al., Cell 75 [1993] 969-976).
- Fc ⁇ RI ⁇ is a heavily glycosylated polypeptide of molecular weight about 60 kD, comprising a hydrophobic transmembrane domain as well as hydrophilic extracellular ( "ecto-" ) and cytoplasmic domains which are exposed to the outer surface of the cell.
- the IgE binding capability of Fc ⁇ RI ⁇ has been further localized to its extracellular portion (J. Hakimi et al. [ 1990], supra; Leder et al., USP 4'962'035). It is possible to produce a soluble, secretable molecule by excising the transmembrane portion and sequences downstream therefrom (C. Ra et al., Int. Immunol.
- truncations of Fc ⁇ RI ⁇ consisting essentially of the extracellular domain can potentially be administered therapeutically to a mammal to bind serum IgE in order to prevent its binding to its high affinity receptor on allergy effector cells, and also for suppressing de novo IgE biosynthesis in human lymphocytes (Y. Yanagihara et al., J. Clin. Invest. 94 [ 1994] 2162-2165).
- an IgE-binding.polypeptide such as the extracellular domain of Fc ⁇ RI ⁇ for systemic treatment of IgE- or IgE receptor- mediated allergic disorders in mammals has been hindered by its extreme transience in vivo due to rapid clearance from circulating plasma.
- IgE- or IgE receptor- mediated diseases account for 10-20 % of physician-patient contact
- an effective treatment would constitute a significant benefit to patients suffering from such conditions and an important advance in the clinical treatment of IgE- and IgE receptor- mediated disorders such as allergy and allergy-related conditions.
- IgE-binding polypeptide having prolonged effective serum life and thus improved clinical utility in the treatment of allergy, and in particular the systemic treatment of atopic dermatitis, atopic asthma and chronic urticaria, as well as improved activity in an efficient, cost-effective manner.
- IgE-binding domain to a human serum albumin (HSA) component
- HSA human serum albumin
- fusion polypeptides can be obtained having extended serum half-life relative to the IgE-binding domain alone, without loss of IgE-binding activity, resulting in IgE-binding polypeptides indicated for use in the systemic treatment of allergy and other IgE-mediated disorders.
- Systemically administered IgE-binding polypeptide will bind to serum IgE as well as to circulating auto-antibodies against the IgE receptor, Fc ⁇ RI ⁇ , preventing them from binding to cell-bound Fc ⁇ RI ⁇ , and thus preventing and/or inhibiting an allergic reaction and its associated manifestations.
- fusion polypeptides of the invention comprising more than one IgE- bmding domain per molecule.
- a "dimer" molecule of the invention has been found to have significantly increased IgE-binding activity relative to a "monomer” of the invention.
- the term “dimer” herein refers to a fusion polypeptide of the invention possessing two IgE-binding domains.
- the term “monomer” refers to a fusion polypeptide of the invention possessing one IgE binding domain.
- the monomer or dimer may be constructed of a single or multiple HSA components.
- a monomeric fusion polypeptide of the invention may comprise an IgE-binding domain fused at either its amino or carboxy terminus to an HSA component.
- the monomer may comprise an IgE-binding domain fused at both of its termini to HSA components.
- a dimeric fusion polypeptide according to the invention may comprise, for example, two IgE-binding domains fused via the carboxy terminus of one and the amino terminus of the other to an intervening HSA component.
- the dimer may comprise, in addition to its two IgE-binding domains, multiple HSA components.
- dimer molecule of the invention possesses unexpectedly favorable activity.
- the invention therefore is directed to fusion polypeptides and salts thereof comprising at least one IgE-binding domain fused to at least one HSA component; preferabh . to monomeric fusion polypeptides, wherein a single IgE-binding domain is fused to one or more HSA components, or to multimeric fusion polypeptides, wherein two or more IgE-binding domains are fused to at least one HSA component; more preferably, dimers possessing two IgE-binding domains and at least one HSA component.
- the invention is further directed to polynucleotide intermediates therefor.
- fused refers to polypeptides in which: (i) a given functional domain (i.e. an IgE-binding domain) is bound at its carboxy terminus by a covalent (preferably, peptide, i.e. amide) bond either to the amino terminus of another functional domain (i.e. an HSA component) or to a linker peptide which itself is bound by a covalent (preferably, peptide) bond to the amino terminus of the other functional domain; and/or,
- a given functional domain i.e. an IgE-binding domain
- a covalent (preferably, peptide, i.e. amide) bond either to the carboxy terminus of another functional domain (i.e. an HSA component) or to a linker peptide which itself is bound by a covalent (preferably, peptide) bond to the carboxy terminus of the other functional domain.
- fused when used in connection with the polynucleotide intermediates of the invention means that the 3'- [or 5'-] terminus of a nucleotide sequence encoding a first functional domain is bound to the respective 5 '-[or 3'-] terminus of a nucleotide sequence encoding a second functional domain, either by a covalent bond or indirectly via a nucleotide linker which itself is covalently bound preferably at its termini to the first functional domain-encoding polynucleotide and the second functional domain-encoding polynucleotide.
- the fusion polypeptide or its salt is a monomer or a dimer; when it is a dimer it comprises two IgE-binding domains fused to an HSA component, such as a first IgE-binding domain fused at its carboxy terminus to the amino terminus of an HSA component, and a second IgE-binding domain fused at its amino terminus to the carboxy terminus of that HSA component.
- the fusion polypeptides of the invention may also comprise further functional domains in addition to IgE-binding domains and HSA components, e.g. full length or truncated (e.g. soluble, extracellular fragments of) human proteins, such as cytokines or the amino-terminal fragment of urokinase.
- additional functional domains may themselves serve as linker peptides, for example, for joining an IgE-binding domain to another IgE-binding domain or to an HSA component; alternatively, they may be located elsewhere in the fusion molecule, e.g. at the amino or carboxy terminus thereof.
- fusion polypeptides of the invention may be represented by the following formulas:
- R is the amino acid sequence of an IgE-binding domain
- R is the amino acid sequence of an HSA component
- each L is independently a covalent (preferably, peptide) bond, or is a peptide linker which is bound by a covalent (preferably, peptide) bond to a terminus of R, and/or R 2 , whereby the above molecule fragments are read directionally, i.e. with the left side corresponding to the amino terminus and the right side to the carboxy terminus of the molecule.
- IgE binding is at high levels when the fusion polypeptide is led by an IgE-binding domain; that is, when the IgE binding domain constitutes the N-terminal portion of the mature fusion protein (such as in compounds of formulae I, III and IV, above).
- a particularly preferred embodiment of the invention comprises a dimer of formula III above, i.e.
- the fusion polypeptides of the invention comprise at least one soluble, secretable mammalian IgE-binding domain fused to at least one HSA component, and pharmaceutically acceptable salts thereof.
- the invention is further directed to a method for the prevention and/or treatment of IgE- or IgE-receptor- mediated disorders, and in particular, allergic reactions such as atopic dermatitis, atopic asthma and chronic urticaria, comprising the administration of the fusion polypeptides of the invention or pharmaceutically acceptable salts thereof to a subject in need of such treatment; and to pharmaceutical compositions comprising the fusion peptides of the invention or pharmaceutically acceptable salts thereof, together with at least one pharmaceutically acceptable carrier or diluent
- the invention is further directed to physiologically functional equivalents of the fusion polypeptides of the invention which are intermediates in the synthesis of the polypeptides; to polynucleotides which are intermediates in the preparation of the fusion polypeptides or physiologically functional equivalents thereof as defined above; to oligonucleotide intermediates to be used to construct them; to the peptides encoded by such oligonucleotides; to recombinant vectors for expressing the fusion molecules; to procaryotic or eucaryotic (especially, mammalian) expression systems; and to processes for preparing the fusion polypeptides or physiologically functional equivalents thereof using such expression systems.
- SEQ. ID. NO. 1 Amino acid sequence of dominant form of full length native human Fc ⁇ RI ⁇ , including signal sequence.
- pre-IgE R refers to residues Met j -Leu 2 o4 of SEQ. ID. NO. 1.
- IgE R refers to the mature form of pre-IgE R and constitutes residues Val o6 -Leu 2 o4 of SEQ ID NO. 1 (i.e. the extracellular domain of Fc ⁇ RI ⁇ ).
- prepro HSA I Amino acid sequence of dominant form of native prepro-HSA (referred to herein as "prepro HSA I"), comprising residues Met Leu 609 .
- HSA I The dominant form of the mature native protein (referred to herein as "HSA I") is represented by residues Asp 2 5-Leu 6 o9 of SEQ. ID. NO. 2.
- prepro-HSA II represents a truncation of the native sequence by one ammo acid (Leu 609 ) at the carboxy terminus, and therefore refers to residues Met
- HSA II The mature form of prepro-HSA II, referred to herein as "HSA II", is represented by residues Asp : Gly 608 of SEQ. ID NO. 2
- a mature dimeric fusion polypeptide of the invention referred to herein as IgE R - L,- HSA II - L 2 • IgE R or, alternatively, as "IgE R - HSA - IgE R Dimer", expressed from CHO cells in the manner described in Example 7, has the amino acid sequence Val 26 -Leu 978 of SEQ. ID. NO. 3.
- SEQ. ID. NO. 4 Nucleotide sequence of the EcoRI fragment of plasmid R-H-R SK #50 of Example 5, comprising: a polynucleotide sequence encoding "pre-IgE R " at positions 10-621 ; an oligonucleotide encoding L j at positions 622-642; a polynucleotide encoding HSA II at positions 643-2394; an oligonucleotide encoding L 2 at positions 2395-2406; and a polynucleotide encoding "IgE R " at positions 2407-2943; with 2 stop codons at positions 2944-2949.
- Restriction sites at the ends of the coding fragments and in the linker regions are at positions 1-6; 622-627; 637-642; 2387-2393; 2401-2406; and 2950-2955.
- a Kozak sequence is at nucleotide positions 7-9.
- Point mutations differing from the consensus HSA nucleotide sequence are at positions 804, 1239; 1290; 1446, 1815, 2064 and 2079. Because the point mutations are in the wobble position, they do not affect the amino acid sequence.
- Seq. ID. NO. 5 Nucleotide sequence of the dominant form of native prepro-HSA corresponding to FIG. 12 and to the amino acid sequence of SEQ.ID.NO.2.
- Seq. ID. NO. 6 Nucleotide sequence of the dominant form of full length native human Fc ⁇ RI ⁇ , including signal sequence, corresponding to FIG. 13 and to the amino acid sequence of SEQ.ID.NO. 1.
- the indicated molecules are read directionally, i.e. the left side corresponding to the ammo (or 5'-) terminus and the right side corresponding to the carboxy (or 3'-) terminus.
- FIGS. 1A-C Serum half life in mice: Protein concentration in vivo (in picomoles of protein per ml of serum) over time ( 10-800 minutes after injection) of
- IgE R protein (referred to as "Free alpha chain”) and IgE R - L, - HSA II - L 2 - IgE R dimeric fusion polypeptide (referred to as "IgE - HSA - IgE R " dimer) prepared from CHO cells as described in Example 7.
- IgE R - L j - HSA 0 - 1 ⁇ - IgE R polypeptide (IgE R - HSA - IgE R Dimer") prepared as desc ⁇ bed in Example 7 (the control group receiving 0 ⁇ g/kg); area in mm ; at intervals of 5, 15 or 30 minutes prior to sensitization by intradermal injection of monoclonal mouse IgE anti-di trophenyl (DNP) antibody and subsequent challenge with DNP-bovme serum albumin solution containing 1 % Evans blue
- FIG. 3 Schematic representation of three fusion polypeptides of the invention (two monomers and one dimer), with the polypeptide linkers also shown
- HSA-leading monomer comprising HSA II (referred to in the Figure as "HSA") fused v ia L ( GGGS”) to IgE R
- HSA HSA II
- GGGS v ia L
- the nucleotides encoding positions Leu ⁇ Gly ⁇ g of HSA II contain a unique Mstll site as indicated, and the nucleotides encoding GlySer of L 7 contain a BamHI site (encoded amino acids underlined),
- IgE-binding domain-leading monomer comprising IgE fused at its carboxy terminus ⁇ u L, ( i e ' ASGGGGS") to HSA II (referred to in the Figure as "HSA")
- HSA II HSA II
- the oligonucleotide encoding L j contains a Nhel site and a BamHI site respectively (encoded amino acids AlaSer and GlySer of L j are underlined).
- Dimer IgE-bindmg domain-leading dimer comprising a first IgE R fused at its carboxy terminus via L j to the amino terminus of HSA II (referred to in the Figure as "HSA"), the carboxy terminus of which is fused to a second IgE R via L with restriction sites in the encoding polynucleotide as desc ⁇ bed above for the monomer.
- HSA II HSA II
- FIG. 4 PCR primers to truncate full length human Fc ⁇ RI ⁇ cDNA (referred to as "IgE Receptor cDNA") to obtain DNA encoding: (i ) IgE R
- oligonucleotide #20 adding SstI, EcoRI, and Kozak sites to 5' end of coding strand for Fc ⁇ RI ⁇ ; oligonucleotide #31 adding NotI and Nhel (and deleting a second Nhel site) in non-coding strand for Fc ⁇ RI ⁇ ]; (iii) pre-IgE R [oligonucleotides #20 and #19 used as described above]:
- HSA-leading subcloning of (i) above into SK vector, providing cloning vector TA clone pEK 1 used in construction of HSA II - leading monomer;
- FIG. 5 PCR primer pairs to truncate full length human prepro-HSA I cDNA to yield cDNA encoding: l i) prepro-HSA II fused at carboxy terminus to L* >
- oligonucleotide # 24 adding Spel, EcoRI and Kozak sequence to 5'-end of coding strand; oligonucleotides #28 and #29 adding a linker encoding MstH and Hindlll sites at the
- oligonucleotide #26 adding NotI, Nhel, linker and BamHI sites to coding strand; oligonucleotide #27 containing Ncol site in non-coding strand];
- oligonucleotide #24 used as above; and oligonucleotide #25 adding stop, EcoRI and Hindlll sites to 3' end of non-coding strand]:
- FIG. 6 Human serum albumin (HSA) sequencing oligonucleotides #1-10, 12, 16, 17. 22 and 30, used to sequence materials cloned in TA and after linkage to IgE-binding fragments.
- HSA Human serum albumin
- FIG. 7 IgE receptor sequencing oligonucleotides #1 1. 13 and 23. used to sequence fragments cloned in TA and after linkage to HSA component.
- FIG. 8 is a diagrammatic representation of FIG. 8
- FIG. 9 Construction of vector HSA-IgER SK#49. comprising a polynucleotide encoding prepro-HSA II (referred to in the Figure as "HSA") fused at 3'-term ⁇ nus via oligonucleotide encoding linker LT (represented in FIG.9 by "GGG”) to 5'-term ⁇ nus of polynucleotide encoding IgE (referred to in the Figure as "IgER”), by ligating the BamHI, Sail fragment of pEK l into BamHI.Sall-cut pEK7.
- HSA prepro-HSA II
- GGG oligonucleotide encoding linker LT
- IgER 5'-term ⁇ nus of polynucleotide encoding IgE
- FIG. 10 Construction of vector IgER-HSA/SK#l , comprising a polynucleotide encoding pre-lgE (referred to in the Figure as "IgER") fused at 3'-term ⁇ nus via oligonucleotide for linker L j (represented by "GGG") to 5'-term ⁇ nus of polynucleotide encoding HSA II ("HSA”), by hgating the SstI, Nhel fragment of IgER/TA#l into SstI,NheI - cut HSA/SK#5.
- IgER polynucleotide encoding pre-lgE
- L j represented by "GGG”
- HSA II HSA II
- FIG. 11 Construction of vector HSA-IgER Pst SaI/SK#37. comprising a polynucleotide encoding HSA II (indicated by "HSA3") which is fused at the 3' terminus via oligonucleotide for L 2 (not depicted) to 5'-terminus of polynucleotide encoding IgE R (indicated as "IgER”), by ligating the PstI, Sail fragment of HSA-IgER SK#49 into SK vector.
- HSA3 polynucleotide encoding HSA II
- IgE R indicated as "IgER”
- FIG. 12 Nucleotide sequence of human serum albumin and amino acid sequence corresponding to SEQ. ID. NO. 2 and SEQ. ID. NO. 5.
- FIG. 13 Nucleotide sequence of IgE R and amino acid sequence corresponding to SEQ. ID. NO. 1 and SEQ. ID. NO. 6.
- FIG. 14 Nucleotide and amino acid sequences of the EcoRI fragment of R-H-R SK #50 corresponding to SEQ. ID. NO. 3 and SEQ. ID. NO. 4, encoding the Rl - HSA - Rl dimeric fusion protein
- the HSA sequence is shown in italics
- Linker sequences are shown in lower case.
- Point mutations differing from the consensus HSA nucleic acid sequence are shown in bold lower case. Because the point mutations are in the wobble position, they do not affect the amino acids sequence Rest ⁇ ction sites at the ends of the fragments and in the linker region are underlined.
- FIG. 15 SDS-PAGE of purified mature fusion polypeptide of Example 7-
- the invention concerns fusion polypeptides and salts tiiereof, comprising at least one IgE-binding domain fused to at least one HSA component.
- IgE-binding domain refers to an amino acid sequence capable of binding or otherwise associating with mammalian, e.g. human IgE in a manner preventing the binding of IgE to its receptor, Fc ⁇ RI
- Human Fc ⁇ RI ⁇ encodes an NH 2 -terminal signal peptide [amino acid residues (including the first Met) Met j -Ala 25 ], two lmmunoglobuhn-like extracellular domains (residues Val 26 -Leu 204 ), a hydrophobic transmembrane region (Gln Q5 -Ile 224 ), and a hydrophilic cytoplasmic tail (residues Ser 225 -Asn 257 ) (with reference to SEQ. ID. NO. 1 ).
- the signal peptide is cleaved during intracellular processing.
- the full length amino acid sequence of the dominant form of native human Fc ⁇ RI ⁇ is included herein as SEQ. ID. NO. 1.
- gE R " herein refers to ammo acid sequence Val 26 -Leu 20 of SEQ. ID NO. 1 (encoding the extracellular domain); and the term "pre-IgE R " refers to residues Met ⁇ -Leu 204 of SEQ ID NO 1 (encoding the signal sequence upstream of the extracellular domain)
- the IgE-binding domain is e.g. an amino acid sequence having at least 80%, more preferably at least 85%, more preferably at least 95%, or most preferably at least 99%, homology with IgE as defined above (homology is calculated as the % identity between the native sequence and the altered sequence, as a function of total sequence length of the native molecule, after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent sequence identity).
- the IgE-binding domain component is N-(2-aminoethyl)-2-aminoethyl-N-(2-aminoethyl)-2-aminoethyl-N-(2-aminoethyl)-2-aminoethyl-N-(2-aminoethyl)-2-aminoethyl-N-(2-aminoethyl)-2-binding domain component
- the IgE-binding domain is more preferably
- the IgE-binding domain is even more preferably
- the IgE-binding domain is most preferably IgE R .
- any homolog, truncation or variant is prepared recombinantly as a "secretable" polypeptide, namely the mature peptide sequence encoding the IgE-binding domain can be secreted from the host cell in which it (or a precursor form such as a preform) is synthesized from a polynucleotide.
- human serum albumin constitutes the most abundant plasma protein, contributing 60 % on a per weight basis of the total protein content of plasma.
- a molecule of human serum albumin consists of a single non-glycosylated polypeptide chain of 585 amino acids of molecular weight 66.5 kDa.
- a feature specific to albumin is its complex disulfide bond pattern (F.F. Clerc et al., J. Chromatogr. 662 [1994] 245-259).
- Human serum albumin is widely distributed throughout the body, in particular in the intestinal and blood compartments where it is mainly involved, as the most abundant protein of the serum, in the maintenance of osmolarity and plasma volume.
- Human serum albumin is devoid of enzymatic or immunological function. It is a natural carrier involved in the endogenous transport and delivery of various natural as well as therapeutic molecules (H. Lu et al., FEBS Lett. 356 H9941 56-59; WO 93/15199; EP 648499; P. Yeh et al., PNAS USA 89 [ 19921 1904- 1908; EP 413622).
- Human cells synthesize serum albumin initially in the form of a prepro- polypeptide.
- a signal sequence of 18 amino acids (including the first Met) is removed when the protein passes through the lumen of the endoplasmic reticulum, leaving still 6 amino acids at the N- terminus (in the predominant form: Arg-Gly-Val-Phe-Arg-Arg) which are then subject to proteolytic excision during or immediately following transport through the secretory apparatus
- Human serum albumin is well-known to be polymorphic (D.C. Carter and J.X. Ho, Ad ⁇ Prot Chem 45 [ 1994] 153-203).
- albumin Naskapi has Lys ⁇ in place of Glu- ⁇ -
- proalbumin Ch ⁇ stchurch has an altered pro- sequence, i.e. Glu 24 instead of Arg 24 (Latta et al., USP 5' 100 * 784)
- prepro-HSA I The complete ammo acid sequence of the dominant form of naturally occurring protein, referred to herein as "prepro-HSA I", is known (A. Dugaiczyk et al., PNAS USA 79 [ 1982] 71-75) (SEQ. ID. NO. 2).
- the dominant form of the mature protein (“HSA I") is represented by Asp 5 -Leu ⁇ Q 9 of SEQ. ED. NO. 2.
- the HSA carrier of the fusion polypeptide of the invention may be an amino acid sequence having at least 80 %, more preferably at least 85 %, even more preferably at least 95 %, or most preferably at least 99 %, homology with residues 25-609 of SEQ. ID. NO. 2 (i.e. HSA I) (homology is calculated as the % identity between the native sequence and the altered sequence, as a function of total sequence length of the native molecule, after aligning the sequences and introducing gaps, if necessary, to achieve the maximum percent sequence identity).
- the HSA component is an amino acid sequence having at least 80 %, more preferably at least 85 %, even more preferably at least 95 %, or most preferably at least 99 %, homology with residues 25-609 of SEQ. ID. NO. 2 (i.e. HSA I) (homology is calculated as the % identity between the native sequence and the altered sequence, as a function of total sequence length of the native molecule, after aligning the sequences and introducing gaps
- the HSA component is more preferably
- the HSA component is even more preferably
- HSA II a truncation of HSA I at the carboxy terminus thereof by 1-10 (e.g., 1) amino acids
- HSA II a truncation of HSA I at the carboxy terminus thereof by 1-10 (e.g., 1) amino acids
- HSA II a truncation of HSA I at the carboxy terminus thereof by 1-10 (e.g., 1) amino acids
- HSA II a truncation of HSA I at the carboxy terminus thereof by 1-10 (e.g., 1) amino acids
- HSA II is the amino acid sequence represented by Asp 25 -Gly 608 of SEQ. ID. NO. 2.
- the HSA component is HSA I or HSA II, particularly HSA II.
- fusion polypeptide of Example 7 especially without leader sequence, i.e. the polypeptide Va ⁇ g- eugyg of SEQ.ID.NO.3.
- any homolog, truncation or variant of native HSA which is used to prepare a fusion protein of the invention will be devoid of enzymatic function; and will not prevent or inhibit binding of the IgE-bindmg domain to serum IgE. It is further preferred that any such homolog or variant will have a serum half-life of at least 14 days
- substitutions refers to the substitution of one or more amino acids by others having similar properties such that one would expect at least the secondary structure, and preferably the tertiary structure of the polypeptide, to be substantially unchanged
- typical such substitutions include alanme or valine for glycine, asparagine for glutamine. se ⁇ ne for threonine and arginine for lysine All of the amino acids (except for ghcine ) are preferably naturally-occurring L-amino acids
- each IgE-bindmg domain is at least 95% homologous to IgE R
- each HSA component is at least 95% homologous to HSA I.
- each IgE-bindmg domain is preferably (Xa), and each HSA component is (Ya), or more preferably (Yb), or even more preferably (Yc), as defined above;
- each IgE-bindmg domain is more preferably (Xb), and each HSA component is (Ya), or more preferably (Yb), or even more preferably (Yc), as defined above;
- each IgE-binding domain is even more preferably (Xc)
- each HSA component is (Ya) or more preferably (Yb), or even more preferably (Yc).
- the IgE-binding domain is IgE R and the HSA component is (Ya), or more preferably (Yb), or even more preferably (Yc), as defined above, and is most preferably HSA I or HSA II, in particular HSA II.
- Preferred polypeptides of the invention are those comprising a first molecule of IgE R which is fused via its carboxy terminus to the amino terminus of a molecule of HSA II, which HSA II is fused at its carboxy terminus to the amino terminus of a second molecule of IgE R (IgE R being residues Val 26 -Leu 204 of SEQ. ID. NO. 1; and HSA II being residues Asp 25 -Gly 608 of SEQ. ID. NO. 2).
- Particularly preferred polypeptides of the invention comprise dimers of formula III p hereinabove wherein each R j is IgE and R 2 is HSA II. Especially preferred are the dimers of formula III wherein each L is 1-25 amino acids.
- Any peptide linker (expressed as "L” in formulae I-V herein) preferably allows independent folding and activity of the IgE-binding domain; is free of a propensity for developing an ordered secondary structure which could interfere with the IgE-binding domain or cause an immunological reaction in the patient, and has minimal hydrophobic or charge characteristics which could interact with the IgE-binding domain.
- the peptide linker is preferably 1-500 amino acids; more preferably 1-250; and even more preferably 1- 100 (e.g 1-25, 1-10, 1-7 or 1-4) amino acids.
- the linker is preferably lin ar, i e non-branched
- peptide linkers comprising Gly, Ala and Ser can be expected to satisfy the criteria for a linker.
- linkers of the present invention include GlyGlyGlySer ("L
- the invention also includes oligonucleotides encoding the peptide linkers of the invention.
- oligonucleotides should be "fused in frame” with the polynucleotides encoding the IgE-binding domain and HSA component, and preferably include restriction sites unique in the molecule.
- “fused in frame” is meant that:
- the invention further encompasses physiologically functional equivalents of the novel fusion polypeptides which are normally intermediates in the synthesis of the novel polypeptides.
- physiologically functional equivalent preferably refers to a larger molecule comprising the fusion polypeptide of d e invention to which has been added such amino acid sequence as is necessary or desirable for effective expression and secretion of the mature recombinant fusion polypeptide of the invention from a particular host cell.
- Such added sequence is typically at the amino terminus of the mature polypeptide, and usually constitutes a leader (i.e.
- the signal sequence can be derived from the natural N-terminal region of the relevant polypeptide, or it can be obtained from host genes coding for secreted proteins, or it can derive from any sequence known to increase the secretion of the polypeptide of interest, including synthetic sequences and all combinations between a "pre-" and a "pro-" region
- the juncture between the signal sequence and the sequence encoding the mature polypeptide should correspond to a site of cleavage in the host.
- fusion polypeptides wherein an IgE-binding domain "leads" expression, i.e. is upstream from other coding sequences in the fusion molecule, it is expedient to utilize the leader sequence of native human Fc ⁇ RI ⁇ (i.e. Met ] + Ala ⁇ Ala ⁇ 5 of SEQ. ID. NO. 1 ), and this has been employed effectively to obtain the mature polypeptide from mammalian expression systems (e.g. CHO, COS), as well as from yeast (Pichia pastons).
- mammalian expression systems e.g. CHO, COS
- yeast palia pastons
- an example of a physiologically functional equivalent of the mature dimeric fusion polypeptide lgE R - L, - HSA II - L 2 - IgE R is: pre- IgE R - L, - HSA II - L 2 - IgE R .
- the additional leader sequence is not necessarily that of human Fc ⁇ RI ⁇ and may be obtained from any suitable source, provided it is suitable to effect expression/secretion of the mature polypeptide from the particular host cell.
- the prepro- sequence of HSA may also be used to prepare the above dimeric fusion polypeptides, particularly for expression in yeast.
- a suitable leader sequence may comprise the native leader sequences.
- the native prepro- region of HSA may be used to accomplish secretion of the mature heterologous polypeptide from mammalian (e.g. CHO, COS) cells or yeast (Pichia pastoris).
- An example of a physiologically functional equivalent of the mature fusion polypeptide represented by HSA II - L 2 - IgE R is: prepro HSA II - L 2 - IgE R .
- other leader sequences not necessarily native to HSA or to the host cell, may provide effective expression of the mature fusion protein in certain hosts (USP 5' 100 * 784; USP 5 * 330 * 901).
- the invention also comprises polynucleotides which are intermediates in the preparation of the recombinant fusion polypeptides of the invention, including polynucleotides encoding the fusion polypeptides or physiologically functional equivalents thereof and oligonucleotides encoding linker peptides, e.g. as depicted in Figure 8.
- a process for preparing a recombinant fusion polypeptide as defined above or salt thereof which comprises:
- a still further aspect of the present invention provides vectors, preferably plasmids, for use in the expression of the fusion polypeptides.
- These vectors comprise DNA encoding the polynucleotides defined above or physiologically functional equivalents thereof.
- appropriate vectors which can transform microorganisms capable of expressing the fusion polypeptides include expression vectors comprising nucleotide sequences coding for the fusion polypeptides joined to transcriptional and translational regulatory sequences, such as promoters, which together constitute an expression cassette.
- the promoters may derive from genes of the particular host used, or such control regions may be modified, for example, by in vitro site-directed mutagenesis, by introduction of additional control elements or synthetic sequences.
- the expression cassette specifically used in the present invention thus also includes a transcription and translation termination region which is functional in the intended host and which is positioned at the 3' end of the sequence encoding the hybrid macromolecule.
- the vector will include one or several markers enabling the transformed host to be selected. Such markers include markers conferring resistance to antibiotics such as G418. These resistance genes will be placed under the control of the appropriate transcription and translation signals allowing for expression in a given host.
- recombinant fusion polypeptides of the invention may be effected e.g. as follows:
- the first step in the construction of recombinant fusion polypeptides is to subclone portions of the fusion polypeptides in cloning vectors.
- a "cloning vector” is a DNA molecule, such as a plasmid, cosmid or bacteriophage, that can replicate autonomously in a host prokaryotic cell.
- Cloning vectors typically contain one or a small number of restriction endonuclease recognition sites at which foreign DNA sequences can be inserted in a determinable fashion without loss of an essential biological function of the vector, as well as a marker gene that is suitable for use in the identification and selection of cells transformed with the cloning vector.
- Marker genes typically include genes that provide tetracycline resistance or ampicillin resistance. Suitable cloning vectors are described in J. Sambrook et al. (eds. ). Molecular Cloning: A Laboratory Manual, 2nd Edition (Cold Spring Harbor Laboratory Press [ 1989]) and can be obtained, for example, from various sources.
- the Fc ⁇ RI ⁇ cDNA clone pGEM-3-1 lOB-l is described in A. Shimizu et al., Proc.Nat.Acad.Sci.USA 85 [ 1988] 1907-191 1 and can be obtained from the American Type Tissue Collection (ATCC stock #67566). Single-stranded human liver cDNA can be obtained from Clontech (PCR-ready Quick Clone cDNA, Cat. D#71 13-l). The sequence of HSA is available from GenBank under Accession #s: VOO495, JOOO78, LOO 132, LOO133. HSA cDNA can be obtained by PCR amplification using oligonucleotides #24 and 25, as described in Example 2.
- a polynucleotide encoding a suitable IgE-binding domain or HSA component DNA can be prepared using the polymerase chain reaction (PCR).
- PCR utilizes a single-stranded cDNA template and a mixture of oligonucleotide primers.
- the PCR procedure is perfomed via well-known methodology (see e.g. C.R.M. Bangham, "The Polymerase Chain Reaction: Getting Started” in Protocols in Human Molecular Genetics, Human Press [1991], Ch.I, p. 1-8).
- PCR kits and material for use in the kits are also commercially available from various sources. Kits and methods for using them are further described in e.g. USP 5 * 487 * 993).
- DNA sequences encoding signal peptides can be added by PCR or if necessary using synthetic oligonucleotides that encode known signal peptide sequences. DNA sequences encoding a heterologous signal peptide are subcloned in frame with DNA sequences encoding the N-terminus of the fusion polypeptide.
- Fusion of the polynucleotides may be accomplished by subcloning in intermediate vectors.
- one gene can be cloned directly into a vector containing the other gene.
- Linkers and adapters can be used for joining the DNA sequences.
- Subcloning is performed in accordance with conventional techniques, such as by use of restriction enzyme digestion to provide appropriate termini, the use of alkaline phosphatase treatment to avoid undesirable joining of DNA molecules, and ligation with appropriate ligases. Techniques for such manipulation are described in the literature and are known in the art.
- the cloned fusion protein is then cleaved from the cloning vector and inserted into an expression vector.
- Suitable expression vectors typically contain
- prokaryotic DNA elements coding for a bacterial replication origin and an antibiotic resistance marker to provide for the growth and selection of the expression vector in a bacterial host;
- DNA elements that control the processing of transcripts such as a transcription termmation/polyadenylation sequence.
- another aspect of the present invention concerns vectors, preferably plasmid vectors, for use in the expression of the fusion polypeptides of the invention, which contain the polynucleotide sequences described herein which code for the fusion polypeptides of the invention.
- Appropriate expression vectors which can transform procaryotic or eucaryotic cells include expression vectors comprising nucleotide sequences coding for the fusion molecules joined to transcriptional and translational regulatory sequences which are selected accoding to the host cells used.
- vectors such as that described by M.W. Robertson, J. Biol. Chem. 268 [1993] 12736-12743 can be used.
- the product is expected to be disulfide-bonded, but not glycosylated.
- yeast an expression vector such as pHIL-D2 supplied with the Invitrogen (San Diego, CA, USA) Pichia pastoris expression kit (catalogue # Kl 710-01) can be used.
- the protein product is expected to be disulfide-bonded and glycosylated.
- yeast expression systems include Saccharomyces cerevisiae and Kluveromvces lactis.
- vectors such as pAC360, pVL1392 and pVL1393 (Invitrogen, San Diego, CA, USA) are useful for infection of insect cells, which would be expected to secrete a glycosylated and disulfide-bonded product.
- the fusion polypeptide of the invention normally is a glycoprotein, particularly when expressed in mammalian cells, and the invention includes fusion polypeptides in any glycosylation, or disulfide bridging, state.
- mutational analysis suggests that N-l inked glycosylation at the first, second and seventh positions of the N-linked glycosylation sites of the IgE receptor ⁇ chain (A. Shimizu et al., PNAS USA 85 [1988] 1907- 191 1 , Figure 2) (corresponding to amino acid residues 46, 67 and 191 of SEQ. ID. NO.1 ), promotes biological activity of the IgE molecule and monomers and dimers comprising it.
- the most preferred expression system is one in which any sugars added will be most similar to those in the native molecule from which the polypeptide is derived, ".'east and insect cells are known to modify glycoproteins differently from mammalian cells, whereas E. coli does not add sugar molecules after secretion. Therefore, while expression from any of these expression systems can yield a protein product which is useful for diagnostic applications (for example, the detection of an allergic condition), the most preferred form for expressing this product for use as a therapeutic molecule is expression in mammalian cells or possibly expression in the milk of transgenic mammals.
- the transcriptional and translational regulatory signals used in an expression cassette may be derived from viral sources, such as adenovirus, bovine papilloma virus or simian virus, in which the regulatory signals are associated with a particular gene which has a high level of expression.
- viral sources such as adenovirus, bovine papilloma virus or simian virus
- Suitable transcriptional and translational regulatory sequences also can be obtained from mammalian genes, such as actin, collagen, myosin, and metallothionein genes.
- Transcriptional regulatory sequences include a promoter region sufficient to direct the initiation of RNA synthesis in a mammalian cell.
- Examples of typical mammalian cells are Chinese hamster ovary (CHO) cells, SV40-transformed monkey kidney cells (COS), HeLa, BHK, NIH Swiss mouse embryo cells, rat, monkey or human fibroblasts, or rat hepatoma cells.
- the preferred method is secretion from CHO cells.
- CHO nor human cells add the ⁇ l,3-linked galactose residues which are typical of expression in murine cells such as C127 and SP2/0 cells. Antibodies to this sugar linkage are present in human serum [C.F. Goochee et al., BioTechnol. 9 [ 1991] 1347- 1355] and can affect the half-life, accessibility and clearance of recombinant products expressed from these murine cells.
- CHO, dhfr- cells are mutant for dihydrofolate reductase (DHFR), and therefore are unable to synthesize purines, thymidine and glycine de novo.
- DHFR dihydrofolate reductase
- the copy number of chromosomally integrated plasmids bearing wild type copies of the DHFR gene can be increased or amplified by exposing cells transformed with these plasmids to increasing levels of methotrexate, a folate analogue which competes for folate binding at the active site of the enzyme.
- a suitable vector for expression in CHO, dhfr- cells is pMT2 (R.J. Kaufman et al. EMBO J. 6 [ 1987] 187-193)].
- the pMT2 vector has a wild type copy of the DHFR gene which is transcribed as a single mRNA with the foreign gene.
- both the foreign gene and the DHFR gene are co-amplified.
- the product secreted from these cells is expected to be disulfide-bonded and glycosylated in a mammalian pattern.
- An expression vector can be introduced into host cells using a variety of techniques including calcium phosphate transfection, liposome-mediated transfection, electroporation, and the like.
- transfected cells are selected and propagated wherein the expression vector is stably integrated in the host cell genome to produce stable transformants.
- the cells can be cultured, for example, in DMEM media.
- the polypeptide secreted into the media can be recovered by standard biochemical approaches following transient expression 24 - 72 hours after transfection of the cells or after establishment of stable cell lines following selection by e.g. antibiotic resistance.
- Conventional methods for recovering an expressed polypeptide from a culture include fractionation of the polypeptide-containing portion of the culture using well known biochemical techniques. For instance, the methods of gel filtration, gel chromatography, ultrafiltration, electrophoresis, ion exchange or affinity chromatography, such as are known for protein fractionations, can be used to isolate the expressed proteins found in the culture.
- conventional immunochemical methods such as immunoaffmity or immunoabsorption can be performed. Techniques for transformation, culture, amplification, screening and product production and purification are also well-known (see e.g. J. Sambrook et al. [1989], supra; R.J. Kaufman, Genetic Engineering: Principles and Methods, Plenum Press, Vol. 9 [1987] 156-198).
- the fusion polypeptides of the invention are indicated for use therapeutically to treat mammalian, and in particular, human, patients suffering from allergies.
- the IgE-binding domain of the fusion polypeptides competes for IgE with the IgE receptor naturally present on mast cells, basophils and Langerhans cells, so that IgE is bound to the administered protein and unable to bind to these allergy effector cells to mediate the allergic response.
- the IgE-binding domain also competes with the IgE receptor, Fc ⁇ RI, by binding to auto- antibodies to Fc ⁇ RI.
- the present invention provides a pharmaceutical composition for competitively binding IgE (and/or autoantibodies to Fc ⁇ RI), and/or inhibiting production of IgE. and thus for suppressive and/or preventative treatment of IgE- or IgE-receptor- mediated disorders, and more specifically, allergy and conditions associated with allergy, such as atopic dermatitis, atopic asthma and chronic urticaria.
- IgE- or IgE receptor- mediated disorders is meant disorders associated with the binding of the cell-bound IgE receptor, Fc ⁇ RI, to IgE or to auto-antibodies to Fc ⁇ RI, e.g type allergic reactions ("hyper-IgE syndrome") such as bronchial asthma, atopic asthma, hay fever, pollen allergy, allergic rhinitis, atopic dermatitis, eczema, anaphylaxis, as well as chronic urticaria, and also non-allergic Kimura's disease, and other pulmonary, dermatological or autoimmune diseases.
- hyper-IgE syndrome such as bronchial asthma, atopic asthma, hay fever, pollen allergy, allergic rhinitis, atopic dermatitis, eczema, anaphylaxis, as well as chronic urticaria, and also non-allergic Kimura's disease, and other pulmonary, dermatological or autoimmune diseases.
- atopic dermatitis one of the most dramatic manifestations of atopy, is a chronic inflammatory skin disease associated with high serum IgE levels and a sensitization to various environmental allergens (M.-A. Morren et al., J. Am. Acad. Dermatol. 31 [1994] 467-473).
- Current treatment of atopic dermatitis concentrates on the use of steroid- containing creams, and severe cases of atopic dermatitis have been successfully treated with cyclosporin A (H. Granlund et al., Br. J. Dermatol. 132 [1995] 106-112), but the side effects restrict this treatment to a minority of the patients.
- the polypeptides of the invention can be used to treat or prevent chronic urticaria (CU), in which mast cell degranulation through activation of Fc ⁇ RI ⁇ plays a role.
- CU chronic urticaria
- the fusion polypeptides of the invention can clear circulating autoantibodies against Fc ⁇ RI ⁇ , in contrast to anti-IgE monoclonal antibodies alternatively proposed for treatment of the disease.
- polypeptides may be administered in the form of a pharmaceutical composition
- a pharmaceutical composition comprising a polypeptide of the invention, preferably an unmodified polypeptide, or a pharmaceutically acceptable salt thereof, in a pharmaceutically acceptable carrier or diluent.
- salt refers in particular to pharmaceutically acceptable salts prepared from pharmaceutically acceptable, non-toxic acids to form acid addition salts of e.g. an amino group of the polypeptide chain, or from pharmaceutically acceptable non-toxic bases to form basic salts of e.g. a carboxyl group of the polypeptide chain.
- Such salts may be formed as internal salts and or as salts of the amino or carboxylic acid terminus of the polypeptide of the invention.
- Suitable pharmaceutically acceptable acid addition salts are those of pharmaceutically acceptable, non-toxic organic acids, polymeric acids, or inorganic acids
- suitable organic acids comprise acetic, ascorbic, benzoic, benzenesulfonic, citric, ethanesulfonic, fuma ⁇ c, gluconic, glutamic, hydrobromic, hydrochloric, isothionic, lactic, maleic, malic, andelic, methanesulfonic, mucic, nitric, oxalic, pamoic, pantothenic, phosphoric, salicylic, succinic, sulfu ⁇ c, tarta ⁇ c and p-toluenesulfonic, as well as polymeric acids such as tannic acid or carboxymethyl cellulose.
- suitable inorganic acids include mineral acids such as hydrochloric, hydrobromic, sulfu ⁇ c, phosphoric and nitric acid.
- suitable inorganic bases for forming salts of a carboxyl group include the alkali metal salts such as sodium, potassium and lithium salts; the alkaline earth salts such as calcium, barium and magnesium salts; and ammonium, copper, ferrous, ferric, zinc, manganous, aluminum and manganic salts. Preferred are the ammonium, calcium, magnesium, potassium, and sodium salts.
- Examples of pharmaceutically acceptable organic bases suitable for forming salts of a carboxyl group include organic amines, such as t ⁇ methylamine, t ⁇ ethylamine, tri(n-propyl)amme, dicyclohexylamine, ⁇ -(d ⁇ methylam ⁇ no)ethanol, t ⁇ s(hydroxymethyl)a ⁇ unomethane, triethanolamine, ⁇ -(diethylam ⁇ no)ethanol, arginine, lysine, histidien, N-ethylpiperidine, hydrabamine, choline, betame, ethylenediamine, glucosamine, methylglucamine, theobromine, purines, piperazines, piperidines, caffeine and procaine.
- organic amines such as t ⁇ methylamine, t ⁇ ethylamine, tri(n-propyl)amme, dicyclohexylamine, ⁇ -(d ⁇ methylam ⁇ no)ethanol, t ⁇ s(hydroxymethyl
- Acid addition salts of the polypeptides may be prepared in conventional manner by contacting the polypeptide with one or more equivalents of the desired inorganic or organic acid such as hydrochloric acid.
- Salts of carboxyl groups of the peptide may be conventionally prepared by contacting the peptide with one or more equivalents of a desired base such as a metallic hydroxide base, e.g. sodium hydroxide; a metal carbonate or bicarbonate base such as sodium carbonate or sodium bicarbonate; or an amine base such as t ⁇ ethyla ⁇ une or triethanolamine.
- compositions comprising a novel fusion polypeptide as defined above or a pharmaceutically acceptable salt thereof, together with a pharmaceutically acceptable carrier or diluent.
- the carrier is preferably a sterile, pyrogen-free, parenterally acceptable liquid.
- Water, physiological saline, aqueous dextrose, and glycols are preferred liquid carriers or diluents, particularly (when isotonic) for in j ectable solutions
- the composition may be a conventionally prepared lyophi zate
- compositions can be administered systemically, i.e. parenterally (c g intramuscularly, intravenously, subcutaneously or intradermally), or by lntrape ⁇ toneal administration
- parenterally c g intramuscularly, intravenously, subcutaneously or intradermally
- lntrape ⁇ toneal administration it is preferred that the fusion polypeptides be essential!) soluble in patient serum or plasma, e.g. that at least 1 milligram of polypeptide is soluble in one milliner of serum or plasma.
- compositions can also be administered by known techniques for topical administration.
- suitable dosage forms include sprays, opthalmic solutions, nasal solutions and ointments.
- a spray can be manufactured by dissolving the peptide in an appropriate solvent and putting it in a spray to serve as an aerosol for commonly employed inhalation therapy.
- An opthalmic or nasal solution can be manufactured by dissolving the active ingredient in distilled water, adding any auxiliary agent required, such as a buffer, isotonizing agent, thickener, preservative, stabilizer, surfactant or antiseptic, adjusting the mixture to pH 4 to 9.
- Ointments can be obtained e.g.
- composition by preparing a composition from a polymer solution, such as 2 % aqueous carboxyvinyl polymer, and a base, such as 2 % sodium hydroxide, mixing to obtain a gel, and mixing with the gel an amount of purified fusion polypeptide.
- a polymer solution such as 2 % aqueous carboxyvinyl polymer
- a base such as 2 % sodium hydroxide
- the composition is preferably administered subcutaneously or intravenously, and most typically, subcutaneously.
- the invention also comprises a method of treatment of allergic conditions comprising administration of a therapeutically effective amount of a fusion polypeptide of the invention or a pharmaceutically acceptable salt thereof to a patient in need of such treatment
- the method of treatment is practiced during the course of an allergic disease state (1 e when the relief of symptoms is specifically required); or as a continuous or prophylactic treatment (i.e. prior to the onset of an anticipated IgE- or IgE-receptor - mediated disorder, such as an allergic reaction).
- the effective dosage in accordance herewith can vary over a wide range taking into consideration e g the degree or seventy of the condition being treated, the age, sex and condition of the subject, the length of treatment, and the potency of the particular fusion protein, factors which are determinable by conventional methods.
- the patient may be treated on a daily basis in single or multiple ddministration
- the composition may also be administered on a per month basis (or at such ee h intervals as may be appropriate), also in either single or multiple administrations
- a suitable monthly dosage can range from a lower dosage of about 0.5 mg per month to an upper dosage of about 500 mg per month, preferably of from about 1 mg to about 300 mg, more preferably of from about 20 mg to about 250 mg, per month, of the fusion polypeptide of the invention, such as the dimer of Example 7, conveniently administered in divided dosages once or twice a month.
- Higher dosage ranges, e.g. 500 mg per month to 2 g per month, may be indicated in patients having high serum IgE concentrations or in early treatment phases.
- the dosage and timing of administration may vary. Initial administrations of the composition may be at higher dosages within the above ranges, and administered more frequently than administrations later in the treatment of fhe disease. Appropriate dosages may be determined by measuring the content of IgE and antibodies to Fc ⁇ RI in the patient's serum, as indicated above For example, early in the course of disease, the fusion polypeptide of the invention, such as the dimer of Example 7, may be administered in weekly doses of 200-500 mg of polypeptide in the average patient (70 kg). After clearance of serum IgE and antibody to Fc ⁇ RI, the treatment regimen may be reduced to weekly treatments or treatments every other week, with dosages ranging from 50 ⁇ g to 100 mg of polypeptide per treatment.
- compositions of the present invention can be administered either alone or in combination witl other compounds of the present invention or further pharmaceutical agents such as antihistarrunes or corticosteroids.
- the invention also comprises the use of the fusion polypeptides of the invention in an in vitro diagnostic assay of any standard format, e.g. ELISA, to determine the level of IgE or auto-antibodies to Fc ⁇ RI (e.g. in chronic urticaria patients) in a biological sample obtained from a human patient, e.g blood or tissue samples.
- an in vitro diagnostic assay of any standard format, e.g. ELISA, to determine the level of IgE or auto-antibodies to Fc ⁇ RI (e.g. in chronic urticaria patients) in a biological sample obtained from a human patient, e.g blood or tissue samples.
- the HSA component advantageously facilitates binding and detection of IgE or autoantibodies to Fc ⁇ RI in an ELISA formatted assay
- the amount of IgE or auto-antibodies present in the sample can sen e as a measure of the allergic response of the patient to a substance to which the patient has been exposed IgE or auto-antibody levels can also be measured to determine the cfficiencN of anti-allergy therapies, and to monitor a patient's allergic status over time
- the invention further comprises a method of performing gene therapy in humans using a polynucleotide encoding a fusion polypeptide of the invention, for treatment of IgE- or IgE receptor- mediated disorders
- the gene therapy method comprises modifying cells of a patient by introducing therein a polynucleotide encoding a fusion polypeptide of the invention and expressing the polypeptide from such cells. For example, somatic cells may first be removed from a patient, then genetically modified in culture by insertion of the polynucleotide, and the resultant modified cells remtroduced into the patient, whereby a polypeptide of the invention is expressed by the cells of the patient.
- the cells may be modified in vivo by direct insertion of vector DNA encoding the polypeptide.
- Suitable cells for modification include endothelial cells or leukocytes.
- the polynucleotide of the invention is preferably under the control of a regulable (e.g. inducible) promoter. Expression of the polypeptide may thereby be made dependent on exposure of the patient to an exogenous factor using known regulable promoter systems
- the invention also includes utilizing the methods of gene therapy to prepare non-human somatic recombinant or transgenic animals expressing the fusion polypeptides of the invention, e.g. into milk.
- Such modified non-human animals also form part of the invention. Examples of useful animals include mice, rats, rabbits, pigs, sheep, goats and cattle, all of which have been made transgenic using standard techniques (e.g., D.R.
- the animals may be used for modelling purposes or actual production of a protein.
- the invention concerns a transgenic mouse, goat, cow or pig expressing a fusion polypeptide of the invention. Methods for making such animals are well known.
- a polynucleotide encoding the fusion protein of the invention can be introduced into the somatic cells of the animals, in vitro or in vivo, to produce somatic recombinant animals which are modified genetically but which cannot pass the genetic modification on to offspring.
- the polynucleotide can be inserted into cells of embryos for production of transgenic animals able to pass on the capability of expressing the proteins of the invention to offspring.
- Transfection of cells for gene therapy can be accomplished in conventional manner, e.g. by electroporation, calcium phosphate precipitation, a lipofectin-based procedure, intramuscular injection, microinjection or through use of a "gene gun.”
- Plasmid-based v ectors preferably contain a marker such as the neomycin gene for selection of stable transfectants with the cytotoxic aminoglycoside G418 in eukaryotic cells.
- Infection is accomplished by incorporating the genetic sequence for the fusion polypeptide into a retroviral vector.
- Various procedures are known in the art for such incorporation.
- One such procedure which has been widely used employs a defective murine retrovirus for packaging the retrovirus, and an amphotropic packaging cell line to prepare infectious amphotropic virus for use in infecting the target donor cells.
- mice Female SKHl/hr/hr Charles River mice weighing approximately 25 g are injected intravenously with test proteins diluted in sterile Ca +2 , Mg + - free PBS. The mice are divided into groups as follows:
- Serum concentration levels of the various proteins are determined by a) IgE receptor ELISA binding assay; b) inhibition ELISA; or c) HSA Sandwich ELISA, as follows: a) IgE ELISA binding assay:
- IgE serum concentration is determined by detection of IgE binding by the following Sandwich ELISA: 200 ng human IgE is immobilized in COSTAR Strip Plate-8 wells (Cambridge. MA, USA) in 100 ⁇ l coating buffer in a humidifed chamber at 4 U C overnight . Each well is washed twice with 300 ⁇ l Ca 2+ , Mg 2+ - free PBS, pH 7.2. The plates are blocked for one hour at room temperature with 200 ⁇ l Ca 2+ , Mg 2+ - free PBS containing 5% BSA (Sigma).
- the wells are washed twice with 300 ⁇ l PBS and incubated with 100 ⁇ l (lng) of a monoclonal anti-human IgE receptor antibody such as 5H5/F8 for one hour at room temperature.
- a monoclonal anti-human IgE receptor antibody such as 5H5/F8 for one hour at room temperature.
- the wells are washed twice with 300 ⁇ l PBST and incubated with 100 ⁇ l of goat anti-mouse IgG-HRP (Biorad, 1:2000 diluted in Ca 2+ , Mg 2+ - free PBS) for 1 hour at room temperature. Plates were washed three times with 300 ⁇ l of PBST, and horse radish peroxidase (HRP) conjugates are detected with 100 ⁇ l of ABTS (Biorad) substrate. The reaction is stopped after 5 minutes with 100 ⁇ l of 3 % oxalic acid. Color intensities are measured with an EASY READER photometer at 405 n
- Fc ⁇ RI ⁇ 100 ng Fc ⁇ RI ⁇ is immobilized in Nunc 96 well Immunoplates (F96 cert Maxisorb) in 100 ⁇ l coating buffer (0.1 M NaHCO 3 , 0.01 % NaN 3 pH:9.6) in a humidified chamber at 4°C overnight. Each well is washed 4 times with 300 ⁇ l PBS, 0.05 % Tween 20 (washing buffer).
- a negative control 50 ⁇ l of mouse serum diluted 1 :25 in PBS, 0.05 % Tween 20, 2 % FCS
- a dilution se ⁇ es of the fusion polypeptide standard e.g IgE R - L - HSAII - L 2 - IgE standard (dilutions from 400 ng/ml to 1 6 ng/ml) or dilution series of the samples are added.
- mice serum dilution in the incubation mixture is 1:50.
- steptavidine-alka ne phosphatase conjugate (Gibco) diluted 1 1000 in dilution buffer is added and incubated for one hour at 37°C.
- the plates are washed 4 times with 300 ⁇ l washing buffer and after addition of 100 ⁇ l substrate ( 1 mg/ml p-nitrophenyl-phosphate in diethanolamine buffer, pH 9 8 [BIORAD]) the reaction is stopped after incubation for 30 minutes at 37 ° C with 50 ⁇ l of 2 M NaOH Optical densities are measured with a BIOMEK-1000 workstation photometer at 405 nm The quantitative evaluation from the standard curves (4-parameter logistic curve fitting) is made with the Bec man IMMUNOFIT ELISA evaluation program Calculation
- HSA-9 monoclonal anti-mouse HSA
- Nunc 96 well Immunoplates F96 cert. Maxisorb
- 100 ⁇ l coating buffer 0.1 M NaHCO 3 , 0.01 % NaN 3 pH 9 6
- 300 ⁇ l washing buffer PBS, 0.05 % Tween 20
- 100 ⁇ l of 1 : 100 diluted mouse serum (PBS, 0.05 % Tween 20, 2 % FCS dilution buffer) as negative control, or 100 ⁇ l standard ( 1 ⁇ g/
- the anti-HSA-Biotin conjugate is purified by immunoaffinity chromatography with CH-Seph4B-HSA, and cross reactivities with mouse serum are removed by immunosorbtion with mouse serum-agarose. After incubation for two hours at 37°C, the plates are washed 4 times with 300 ⁇ l washing buffer, and 50 ⁇ l streptavidin-alkal e phosphatase conjugate (Gibco) diluted 1 : 1000 in dilution buffer is added and incubated for one hour at 37°C.
- the plates are washed 4 times with 300 ⁇ l washing buffer and after addition of 100 ⁇ l substrate (1 mg/ml p-nitrophenylphosphate in diethanola ⁇ une buffer, pH 9.8, BIO-RAD), the reaction is stopped after incubation for 15 minutes at 37°C with 50 ul 2M NaOH.
- Optical densities are measured with a BIOMEK- 1000 workstation photometer at 405 nm
- the quantitative evaluation from the standard curve (4-parameter logistic curve fitting) is made with the Beckman IMMUNOFIT ELISA evaluation program
- Figure 1 (B) shows that the free receptor is only detectable in mouse serum for about 10 minutes, w hereas the dimeric fusion polypeptide is still detectable about 12 hours after administration
- Figure 1(C) shows the relative clearance kinetics for HSA and the dimeric fusion polv peptide After stabilization of tissue-blood distribution in the first 10 minutes, the dimer and HSA show an almost identical clearance curve This confirms that the serum half life of an IgE-binding domain can be substantially prolonged by fusion to HSA
- PCA passive cutaneous anaphylaxis
- Serial dilutions of 10, 50 or 500 ⁇ g/kg of fusion polypeptide, e.g lgE R - L, - HSA II - L 2 - IgE R obtained from CHO cells in Example 7, are intravenously injected into female SKHl/hr hr Charles River mice weighing approximately 25 g at varying intervals prior to sensitization.
- mice Three groups of mice are established depending on the amount of polypeptide injected prior to sensitization (i.e. 10 ⁇ g/kg, 50 ⁇ g/kg or 500 ⁇ g/kg).
- a fourth group of control mice receive 200 ⁇ g/kg PBS intravenously instead of polypeptide.
- the four groups of mice are each divided into three sub-groups, which differ by the interval between intravenous injection of the compound and intracutaneous sensitization with IgE [step (b) below]. The tested intervals are: 5 minutes, 15 minutes and 30 minutes.
- mice are anaesthetized and sensitized into the back skin by 4 intradermal in j ections of 5 ng each monoclonal mouse anti-dinitrophenyl (DNP) IgE antibody in 10ml PBS (BioMakor, Rehovot, Israel).
- DNP monoclonal mouse anti-dinitrophenyl
- PBS BioMakor, Rehovot, Israel
- mice 90 minutes after sensitization, the mice are anesthesized again and challenged by intravenous injection of a solution containing 50 ⁇ g of dinitrophenyl-bovine serum albumin (DNP-BSA) (Calbiochem-Behring, San Diego, USA) containing 1% Evans blue.
- DNP-BSA dinitrophenyl-bovine serum albumin
- De novo chemical synthesis of the primers of the invention can be conducted using any suitable method, such as, for example, the phosphotriester or phosphodiester methods.
- DNA fragments are excised and purified using the QiaEx protocol (Qiagen, Inc., Chatsworth, CA, USA), then subcloned into a TA vector [TA Cloning® Kit (Invitrogen) (product literature, Version 2.2)].
- QiaEx protocol Qiagen, Inc., Chatsworth, CA, USA
- TA Cloning® Kit Invitrogen
- FIG. 8 DNA is sequenced using the Sequenase method (USB, Cleveland, OH, USA). Plasmids and Reagents:
- the Fc ⁇ RI ⁇ cDNA clone, pGEM-3- 1 lOB- 1 (A. Shimizu et al., Proc. Nat. Acad.Sci.USA 85 [ 1988] 1907-191 1 ) is obtained from the American Type Tissue Collection (ATCC stock #67566). Single-stranded human liver cDNA is obtained from Clontech (PCR-ready Quick Clone cDNA, Cat. D#71 13- 1 ). The sequence of HSA is available under GenBank Accession #s VOO495, JOOO78, LOO 132 and LOO 133. Restriction enzymes are obtained from Boehringer-Mannheim or Gibco/BRL.
- Taq DNA polymerase is obtained from Perkin-Elmer Cetus (PECI) or from Boehringer-Mannheim.
- the SK vector is obtained from Stratagene. pHIL-D2 is available from Invitrogen (San Diego, CA, USA; Catalog no. K1710-01 [ 1994]) (see “Pichia Expression Kit - Protein Expression - A Manual of Methods for Expression of Recombinant Proteins in Pichia pastoris - Version 3.0" [December 1994]) (hereinafter referred to as the "Invitrogen Manual”).
- the pXMT3 vector is derived from pMT2 (Sambrook et al. (Eds.) [1989] supra) by cloning the PstI to EcoRI linker from pUC8 (Pharmacia) into the PstI and EcoRI sites of pMT2 (R.J. Kaufman et al. [1987] supra).
- Plasmid pCR2 is ligated separately with each of the PCR amplification products obtained in Examples 1 and 2.
- the ligation reactions are performed using T4 DNA ligase in the following reaction mixture:
- Each reaction mixture is maintained at 15° for 18 hours before being transformed into competent cells.
- Fc ⁇ RI ⁇ cDNA is purified from pGEM-3- 1 lOB- 1 using the Qiagen method. Then: ( A ) For preparing an HSA-leading construct. PCR amplification of Fc ⁇ RI ⁇ cDNA is carried out with oligonucleotides #18 and #19 (FIGS. 4, 8) using the following reaction mixture:
- reaction mixture is overlaid with mineral oil to prevent evaporation, and thermocycled in a Perkin Elmer Cetus DNA thermocycler model 480. Cycling conditions for this reaction are: heat to 95° for 5 minutes, then 30 cycles of 94° for 1.5 minutes, 53° for
- PCR amplification of Fc ⁇ RI ⁇ cDNA is carried out as in (A) above with oligonucleotides #20 and #19 (FIG. 8B). Following electrophoresis, an amplified product of -620 bp is confirmed by ethidium bromide staining. The PCR fragment is excised and subcloned into PCR II vector to provide IgERFL/TA #34, encoding pre-IgE R followed by a stop codon (FIG. 4).
- PCR amplification of full length human serum albumin cDNA is carried out with oligonucleotides #24 and #25 (FIG. 8B), following the general procedure of Example 1(A).
- the resulting clone HSA TA#1 had the sequence most closely resembling the sequence in Genbank.
- seven mutations in the wobble position and one mutation which resulted in a change from lysine to glutamic acid at base 1333 were detected The seven mutations m the wobble position were, base 309 A to T, base 744.
- HSA TA mut #16 The 1.8 kb product is subcloned into pCR2 vector to form HSA TA mut #16, which is verified by DNA sequencing to contain the complete prepro-HSA II sequence.
- HSA/TA mut #16 is subcloned into Bluescript SK as a Spel, Hindlll fragment, yielding HSA/SK #17 (FIG. 5).
- HSA SK#17 is digested with Mstll and Hindlll to remove the nucleotide sequence encoding the 3'-terminal amino acid (Leu 609 ); and an oligonucleotide encoding linker L 2 , as defined above, is introduced at the
- HSA/SK#17 encoding prepro-HSA II is amplified by PCR with oligonucleotides #26 and #27 (FIGS. 5, 8).
- Oligonucleotide #26 removes the prepro sequence from HSA, and adds an oligonucleotide encoding L j at the 5 " end of HSA.
- Oligonucleotide #27 ends at a naturally occurring, unique Ncol site at about nucleotide position 800 of the HSA coding sequence. PCR amplification is earned out according to the procedure in Example 1(A).
- Example 3 Fusion construct HSA-IgER/SK#22 encoding prepro-HSA + Linker + IgE R
- pEK7 (containing prepro-HSA II cDNA + oligonucleotide for L 2 ) and pEKl (containing IgE R ) are digested with BamHI and Sail.
- the obtained 1.8 kb fragment from ⁇ EK7 is phosphatased and then ligated to the 550 Kb fragment obtained from pEKl .
- One positive miniprep, #23, was prepared but was contaminated with another unknown plasmid which prevented recovery of the HSA-IgE R band.
- the 2.4 kb Spel to Sail fragment containing the HSA - IgE fusion and the 2.9 kb fragment containing the vector DNA were purified from miniprep#23 and ligated together, resulting in clone HSA - IgE/SK#49 (FIG. 9) [vector sites were found to be missing from either end of the subcloned region, and accordingly, the 2.4 kb Spel to Sail fragment from HSA-IgE/SK#49 was subcloned into Spel plus Sail - cut Bluescript SK, resulting in HSA-IgE/SK#22 (not shown in the Figures)].
- HSA SK #5 and IgE R /TA #1 are digested with SstI and Nhel.
- the 600 bp fragment from IgE/TA #1 is purified by gel electrophoresis and ligated into the cut and phosphatase- treated HSA SK #5, yielding clone IgE-HSA/SK#l (FIG. 10).
- HSA/IgE R /SK #49 and Bluescript SK are digested with PstI and Sail.
- a 1.2 kb fragment containing the 3' portion of HSA II, the linker and the IgE R sequence are ligated into PstI plus Sail - cut Bluescript DNA.
- HSA-IgE R Pst Sal/SK #37 FIG. 11
- IgE R -HSA/SK#l DNA is digested with PstI and Kpnl, and a 4.8 kb fragment containing the vector, IgE R , linker and 5' half of HSA are isolated, phophatase-treated, and ligated to the 1.2 kb fragment from HSA-IgE R Pst Sal/SK #37.
- the resultant dimeric construct R-H-R/SK #50 is obtained (FIG. 11).
- Example b HSA II - L ⁇ IgE R monomeric fusion polypeptides bv transfection and culture of Pichia pastoris
- Plasmid MB#2 encoding HSA II - L ⁇ - IgE is prepared by cutting the plasmid HSA/SK#49 with EcoRI and isolating the 2.4 kb fragment encoding the fusion protein This fragment is ligated into the unique EcoRI site of me Pichia pasto ⁇ s expression vector pHIL-D2 (Invitrogen), after digestion of plasmid pHIL-D2 with EcoRI and alkaline phosphatase treatment The resultant MB#2 plasmid is linearized by digestion with NotI and transformed into h ⁇ s4 GS 115 cells as desc ⁇ bed in the "Invitrogen Manual" His- transformants are screened for growth on methanol Strains exhibiting slow growth on methanol are grown in minimal glycerol medium as specified in the "Invitrogen Manual" to stationary phase, transferred by cent ⁇ fugation to buffered complex methanol medium and grown for 4 days The supernatant from the cells is then assayed by ELISA for the presence
- Plasmid pXMT3-RI ⁇ -HSA-RI ⁇ (containing the polynucleotide of SEQ. ID. NO. 4 encoding pre- lgE R - L, - HSA II - L 2 -IgE R ) is prepared by digestion of R-H-R/SK #50
- the dhfr-gene located on the pXMT3 plasmid allows selection for recombinant cells in a nucleoside depleted medium.
- 3 days after transfection cells are placed into Alpha- medium (MEM ALPHA MEDIUM without ribonucleosides and deoxyribonucleo- sides/Gibco) containing 10 % dialyzed fetal calf serum (FCSD). After 2 weeks of cultivation, recombinant cell colonies are visible. Cells are kept in Alpha- medium containing 10 % FCSD for 4 additional passages before gene amplification is started.
- methotrexate MTX
- dhfr and the genes linked to it are amplified, resulting in an increased expression of the transgene. Therefore, selection for recombinant cells in a nucleoside-depleted medium is followed by cultivation in the presence of 20 nM MTX in Alpha- medium containing 10 % FCSD. Further amplification is achieved by stepwise increases in methotrexate to 100 nM and 500 nM MTX.
- Protein is produced by seeding pool Tl/3-500 nM in Alpha- medium containing
- a second batch is purified from 2 liter supernatant derived from pool Tl/3-500 nM
- C Cells are seeded at 5x10 cells/ml and the supernatant collected 6 days after seeding.
- the culture supematants from Example 7 are purified by immunoaffinity chromatography on immobilized anti-Fc ⁇ RI monoclonal antibodies (e.g. 5H5-F8 ; mouse IgG 1 ), produced and purified according to standard techniques: a) Preparation of the chromatographic support:
- the monoclonal antibodies are coupled to CNBr-activated Sepharose 4B (Pharmacia, Uppsala, Sweden) at a density of 10 mg antibodies / ml gel according to the manufacturer's instructions. Excess reactive groups are subsequently blocked with ethanolamine and the resin stored in PBS supplemented with 0.02 % NaN 3 until use.
- CNBr-activated Sepharose 4B Pharmacia, Uppsala, Sweden
- the concentration of the dimenc fusion polypeptide is determined by absorption at 280 nm in its native conformation in 30 mM (3-[N-morphol ⁇ no]propane)sulfon ⁇ c acid (MOPS), pH 7.0 and in the denatured form (6 M guanidme.HCl).
- the corresponding molar absorption coefficient is calculated from the number of tryptophan, tyrosine and cystme residues using the tabulated absorption coefficients of these ammo acids in model compounds and corrected for the difference in optical density between folded and unfolded protein
- the fusion protein contains 17 tryptophans, 40 tyrosmes and 21 cystmes, which results in a theoretical extinction coefficient of 150840 M cm "
- the quality of the purified material is assessed by standard SDS-PAGE and by N-termmus automated gas- phase Edman degradation sequencing and mass spectrometry Upon SDS-PAGE (FIG. 15) the polypeptide migrates with an apparent molecular weight of about 140 kDa, reflecting about 28 % glycosylation (theoretical MW without glycosylation. 108'863 61 Da).
- CTAGAGAAGT GCTGTGCCGC TGCAGATCCT CATGAATGCT ATGCCAAAGT GTTCGATGAA 1200
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Priority Applications (13)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SK77-99A SK7799A3 (en) | 1996-07-26 | 1997-07-25 | Fusion polypeptides comprising an ige-binding domain and a hsa component, and their diagnostic and therapeutic uses |
JP50850498A JP3681402B2 (en) | 1996-07-26 | 1997-07-25 | Fusion polypeptide comprising IgE-binding domain and HSA component and its use as diagnostic and therapeutic agent |
EP97940030A EP0917581B1 (en) | 1996-07-26 | 1997-07-25 | Fusion polypeptides com0prising an ige-binding domain and a hsa component, and their diagnostic and therapeutic uses |
IL12809497A IL128094A0 (en) | 1996-07-26 | 1997-07-25 | Fusion polypeptides comprising an ige-binding domain and a hsa component and their diagnostic and therapeutic uses |
NZ333908A NZ333908A (en) | 1996-07-26 | 1997-07-25 | Fusion polypeptides comprising an ige-binding domain and a hsa component, and their diagnostice and therapeutic uses |
CA2261562A CA2261562C (en) | 1996-07-26 | 1997-07-25 | Fusion polypeptides comprising an ige-binding domain and a hsa component, and their diagnostic and therapeutic uses |
AU42025/97A AU722069B2 (en) | 1996-07-26 | 1997-07-25 | Fusion polypeptides comprising an IgE-binding domain and a HSA component, and their diagnostic and therapeutic uses |
DE69738452T DE69738452T2 (en) | 1996-07-26 | 1997-07-25 | FUSION POLYPEPTIDES CONTAINING AN IGE BINDING DOMAIN AND A HSA COMPONENT AND ITS DIAGNOSTIC AND THERAPEUTIC USES |
BR9710605A BR9710605A (en) | 1996-07-26 | 1997-07-25 | Fusion polypeptides comprising an ige-binding domain and a hsa component and their diagnostic and therapeutic applications. |
IL128094A IL128094A (en) | 1996-07-26 | 1999-01-18 | Fusion polypeptides comprising an ige-binding domain and a hsa component, and their diagnostic and therapeutic uses |
PL97331356A PL189415B1 (en) | 1996-07-26 | 1999-01-25 | Fusion-type peptides containing ieg bonding domain and hsa constituent as well as their diagnostic and therapeutic applications |
NO19990328A NO323611B1 (en) | 1996-07-26 | 1999-01-25 | Fusion polypeptide isolated polynucleotide, method of preparation, and vectors, as well as pharmaceutical composition and use of fusion polypeptides for the preparation of drug for the treatment of IgE or IgE receptor-mediated disorders |
HK99105437A HK1020352A1 (en) | 1996-07-26 | 1999-11-24 | Fusion polypeptides comprising an ige-binding domain and a hsa component, and their diagnostic and therapeutic uses |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US69021696A | 1996-07-26 | 1996-07-26 | |
US08/690,216 | 1996-07-26 |
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WO1998004718A1 true WO1998004718A1 (en) | 1998-02-05 |
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Family Applications (1)
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---|---|---|---|
PCT/EP1997/004066 WO1998004718A1 (en) | 1996-07-26 | 1997-07-25 | Fusion polypeptides comprising an ige-binding domain and a hsa component, and their diagnostic and therapeutic uses |
Country Status (28)
Country | Link |
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EP (1) | EP0917581B1 (en) |
JP (1) | JP3681402B2 (en) |
KR (1) | KR100502879B1 (en) |
CN (1) | CN1146664C (en) |
AR (1) | AR008077A1 (en) |
AT (1) | ATE383429T1 (en) |
AU (1) | AU722069B2 (en) |
BR (1) | BR9710605A (en) |
CA (1) | CA2261562C (en) |
CO (1) | CO4650184A1 (en) |
CZ (1) | CZ297634B6 (en) |
DE (1) | DE69738452T2 (en) |
ES (1) | ES2300114T3 (en) |
HK (1) | HK1020352A1 (en) |
ID (1) | ID19420A (en) |
IL (2) | IL128094A0 (en) |
MY (1) | MY120425A (en) |
NO (1) | NO323611B1 (en) |
NZ (1) | NZ333908A (en) |
PE (1) | PE99498A1 (en) |
PL (1) | PL189415B1 (en) |
PT (1) | PT917581E (en) |
RU (1) | RU2209211C2 (en) |
SK (1) | SK7799A3 (en) |
TR (1) | TR199900155T2 (en) |
TW (1) | TW565571B (en) |
WO (1) | WO1998004718A1 (en) |
ZA (1) | ZA976666B (en) |
Cited By (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1999066054A2 (en) * | 1998-06-15 | 1999-12-23 | Genzyme Transgenics Corp. | Erythropoietin analog-human serum albumin fusion protein |
EP1056471A1 (en) * | 1998-01-29 | 2000-12-06 | Tanox, Inc. | TREATING ATOPIC DERMATITIS WITH IgE ANTAGONISTS |
WO2001011010A2 (en) * | 1999-08-09 | 2001-02-15 | Genaissance Pharmaceuticals, Inc. | Drug target isogenes: polymorphisms in the immunoglobulin e receptor i alpha subunit gene |
WO2001091713A1 (en) * | 2000-05-31 | 2001-12-06 | Fresenius Kabi Deutschland Gmbh | Cosmetic composition comprising human serum albumin obtained from transgenic non-human animals |
JP2004528014A (en) * | 2000-12-07 | 2004-09-16 | イーライ・リリー・アンド・カンパニー | GLP-1 fusion protein |
US8927694B2 (en) | 2008-11-18 | 2015-01-06 | Merrimack Pharmaceuticals, Inc. | Human serum albumin linkers and conjugates thereof |
US8962274B2 (en) | 2009-02-27 | 2015-02-24 | Novartis Ag | Methods for selecting eukaryotic cells expressing a heterologous protein |
US8993517B2 (en) | 2001-12-21 | 2015-03-31 | Human Genome Sciences, Inc. | Albumin fusion proteins |
US9345766B2 (en) | 2012-08-30 | 2016-05-24 | Merrimack Pharmaceuticals, Inc. | Combination therapies comprising anti-ERBB3 agents |
EP3192806A1 (en) | 2016-01-13 | 2017-07-19 | Affiris AG | Alpha chain of the high-affinity ige receptor (fceria) |
US20180230236A1 (en) * | 2016-11-09 | 2018-08-16 | North Carolina State University | Treatment of allergic diseases with chimeric protein |
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TWI329129B (en) * | 2001-02-08 | 2010-08-21 | Wyeth Corp | Modified and stabilized gdf propeptides and uses thereof |
WO2012169735A2 (en) * | 2011-06-07 | 2012-12-13 | (주)네오팜 | Complex comprising water-soluble fragments of fcεri and composition comprising same for treating allergic diseases mediated by ige |
RU2506271C2 (en) * | 2011-06-22 | 2014-02-10 | Учреждение Российской Академии Медицинских Наук Научно-исследовательский институт экспериментальной медицины Северо-западного отделения РАМН (НИИЭМ СЗО РАМН) | RECOMBINANT POLYPEPTIDE A2 SELECTIVELY BINDING HSA, RECOMBINANT DNA pa2 CODING HSA-BINDING PART OF POLYPEPTIDE A2, PRODUCER THEREOF - RECOMBINANT STRAIN OF Escherichia coli M15-A2, CONTAINING RECOMBINANT PLASMID DNA pQE 32-pa2, FACILITATING PRODUCTION OF POLYPEPTIDE A2 AND USE OF POLYPEPTIDE A2 FOR DIAGNOSIS OF MICROALBUMINURIA AND SEPARATING HSA FROM BLOOD SERUM |
WO2016178089A1 (en) * | 2015-05-04 | 2016-11-10 | Laboratoire Francais Du Fractionnement Et Des Biotechnologies | Transgenic production of fc fusion proteins |
EP3998277A4 (en) * | 2019-07-08 | 2023-11-22 | Progen Co., Ltd. | Novel il-10 variant protein and use thereof |
CN111773392B (en) * | 2020-05-25 | 2023-04-07 | 河南省生物工程技术研究中心 | Human serum albumin/oligonucleotide nano-particles and preparation method and application thereof |
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WO1990013653A1 (en) * | 1989-04-29 | 1990-11-15 | Delta Biotechnology Limited | Fusion proteins containing n-terminal fragments of human serum albumin |
EP0499112A1 (en) * | 1991-02-11 | 1992-08-19 | F. Hoffmann-La Roche Ag | Antibodies against an IgE receptor |
-
1997
- 1997-07-24 MY MYPI97003371A patent/MY120425A/en unknown
- 1997-07-24 PE PE1997000662A patent/PE99498A1/en not_active Application Discontinuation
- 1997-07-24 AR ARP970103340A patent/AR008077A1/en active IP Right Grant
- 1997-07-25 ID IDP972592A patent/ID19420A/en unknown
- 1997-07-25 CN CNB971976597A patent/CN1146664C/en not_active Expired - Fee Related
- 1997-07-25 DE DE69738452T patent/DE69738452T2/en not_active Expired - Lifetime
- 1997-07-25 AT AT97940030T patent/ATE383429T1/en active
- 1997-07-25 CA CA2261562A patent/CA2261562C/en not_active Expired - Fee Related
- 1997-07-25 EP EP97940030A patent/EP0917581B1/en not_active Expired - Lifetime
- 1997-07-25 IL IL12809497A patent/IL128094A0/en active IP Right Grant
- 1997-07-25 CZ CZ0024699A patent/CZ297634B6/en not_active IP Right Cessation
- 1997-07-25 SK SK77-99A patent/SK7799A3/en unknown
- 1997-07-25 CO CO97042651A patent/CO4650184A1/en unknown
- 1997-07-25 KR KR10-1999-7000621A patent/KR100502879B1/en not_active IP Right Cessation
- 1997-07-25 ES ES97940030T patent/ES2300114T3/en not_active Expired - Lifetime
- 1997-07-25 AU AU42025/97A patent/AU722069B2/en not_active Ceased
- 1997-07-25 RU RU99103326/13A patent/RU2209211C2/en not_active IP Right Cessation
- 1997-07-25 TR TR1999/00155T patent/TR199900155T2/en unknown
- 1997-07-25 WO PCT/EP1997/004066 patent/WO1998004718A1/en active IP Right Grant
- 1997-07-25 PT PT97940030T patent/PT917581E/en unknown
- 1997-07-25 ZA ZA976666A patent/ZA976666B/en unknown
- 1997-07-25 JP JP50850498A patent/JP3681402B2/en not_active Expired - Fee Related
- 1997-07-25 BR BR9710605A patent/BR9710605A/en not_active IP Right Cessation
- 1997-07-25 NZ NZ333908A patent/NZ333908A/en unknown
- 1997-08-08 TW TW086111356A patent/TW565571B/en not_active IP Right Cessation
-
1999
- 1999-01-18 IL IL128094A patent/IL128094A/en not_active IP Right Cessation
- 1999-01-25 NO NO19990328A patent/NO323611B1/en not_active IP Right Cessation
- 1999-01-25 PL PL97331356A patent/PL189415B1/en not_active IP Right Cessation
- 1999-11-24 HK HK99105437A patent/HK1020352A1/en not_active IP Right Cessation
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Cited By (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1056471A1 (en) * | 1998-01-29 | 2000-12-06 | Tanox, Inc. | TREATING ATOPIC DERMATITIS WITH IgE ANTAGONISTS |
EP1056471A4 (en) * | 1998-01-29 | 2001-05-30 | Tanox Inc | TREATING ATOPIC DERMATITIS WITH IgE ANTAGONISTS |
US7101971B2 (en) | 1998-06-15 | 2006-09-05 | Gtc Biotherapeutics, Inc. | Erythropoietin analog-human serum albumin fusion |
WO1999066054A3 (en) * | 1998-06-15 | 2000-04-06 | Genzyme Transgenics Corp | Erythropoietin analog-human serum albumin fusion protein |
US6548653B1 (en) | 1998-06-15 | 2003-04-15 | Genzyme Transgenics Corporation | Erythropoietin analog-human serum albumin fusion |
AU775422B2 (en) * | 1998-06-15 | 2004-07-29 | Gtc Biotherapeutics, Inc. | Erythropoietin analog-human serum albumin fusion |
WO1999066054A2 (en) * | 1998-06-15 | 1999-12-23 | Genzyme Transgenics Corp. | Erythropoietin analog-human serum albumin fusion protein |
WO2001011010A2 (en) * | 1999-08-09 | 2001-02-15 | Genaissance Pharmaceuticals, Inc. | Drug target isogenes: polymorphisms in the immunoglobulin e receptor i alpha subunit gene |
WO2001011010A3 (en) * | 1999-08-09 | 2001-09-13 | Genaissance Pharmaceuticals | Drug target isogenes: polymorphisms in the immunoglobulin e receptor i alpha subunit gene |
WO2001091713A1 (en) * | 2000-05-31 | 2001-12-06 | Fresenius Kabi Deutschland Gmbh | Cosmetic composition comprising human serum albumin obtained from transgenic non-human animals |
JP2011006447A (en) * | 2000-12-07 | 2011-01-13 | Eli Lilly & Co | Glp-1 fusion protein |
JP2004528014A (en) * | 2000-12-07 | 2004-09-16 | イーライ・リリー・アンド・カンパニー | GLP-1 fusion protein |
US7271149B2 (en) | 2000-12-07 | 2007-09-18 | Eli Lilly And Company | GLP-1 fusion proteins |
US9296809B2 (en) | 2001-12-21 | 2016-03-29 | Human Genome Sciences, Inc. | Albumin fusion proteins |
US8993517B2 (en) | 2001-12-21 | 2015-03-31 | Human Genome Sciences, Inc. | Albumin fusion proteins |
US9221896B2 (en) | 2001-12-21 | 2015-12-29 | Human Genome Sciences, Inc. | Albumin fusion proteins |
US8927694B2 (en) | 2008-11-18 | 2015-01-06 | Merrimack Pharmaceuticals, Inc. | Human serum albumin linkers and conjugates thereof |
US8962274B2 (en) | 2009-02-27 | 2015-02-24 | Novartis Ag | Methods for selecting eukaryotic cells expressing a heterologous protein |
US9315844B2 (en) | 2009-02-27 | 2016-04-19 | Novartis Ag | Methods for selecting eukaryotic cells expressing a heterologous protein |
US9345766B2 (en) | 2012-08-30 | 2016-05-24 | Merrimack Pharmaceuticals, Inc. | Combination therapies comprising anti-ERBB3 agents |
EP3192806A1 (en) | 2016-01-13 | 2017-07-19 | Affiris AG | Alpha chain of the high-affinity ige receptor (fceria) |
WO2017121842A1 (en) | 2016-01-13 | 2017-07-20 | Affiris Ag | Alpha chain of the high-affinity ige receptor (fceria) |
US20180230236A1 (en) * | 2016-11-09 | 2018-08-16 | North Carolina State University | Treatment of allergic diseases with chimeric protein |
US11352443B2 (en) * | 2016-11-09 | 2022-06-07 | North Carolina State University | Treatment of allergic diseases with chimeric protein |
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