WO1998001582A1 - Method for measuring viral infectivity - Google Patents
Method for measuring viral infectivity Download PDFInfo
- Publication number
- WO1998001582A1 WO1998001582A1 PCT/US1997/011865 US9711865W WO9801582A1 WO 1998001582 A1 WO1998001582 A1 WO 1998001582A1 US 9711865 W US9711865 W US 9711865W WO 9801582 A1 WO9801582 A1 WO 9801582A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- virus
- cells
- antibody
- ratio
- polypeptide
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
Definitions
- a particular challenge in the delivery of a gene by a viral vector for therapeutic purposes is the preparation and accurate characterization of clinical dosage forms.
- Total particle measurement can be made by such techniques as electron microscopy of viral preparations or measurement of total DNA by optical density at 260 nm of a sodium dodecyl sulfate (SDS) treated virus suspension.
- SDS sodium dodecyl sulfate
- infectivity of a viral preparation i.e., the number of infectious viral particles in a preparation of virus, is more challenging to accurately measure.
- infectivity particles are measured in culture by a plaque-forming unit assay (pfu) that scores the number of viral plaques as a function of dilution.
- pfu plaque-forming unit assay
- An alternative to the pfu assay is the tissue culture infective dose procedure (TCID 50 ) , which estimates infectivity as a function of intracellular staining for an antigen by direct im unofluorescence.
- TCID 50 tissue culture infective dose procedure
- the methods suffer from limitations including a high degree of inter-assay variability and are affected by such factors as virus replication status, vector characteristics, and virus-cell interactions.
- flow cytometry or FACS fluorescence- activated cell sorter
- FACS fluorescence- activated cell sorter
- Saalm ⁇ ller and Mettenleiter disclose the identification and quantitation of cells infected by recombinant pseudorabies virus mutants by the reaction of intracellular ⁇ -galactosidase expressed during infection with recombinant viruses with a fluorogenic substrate, followed by detection of positive cells in flow cytometry.
- One aspect of the invention is a method for determining the number of infectious virus particles in a population of virus particles comprising: i) infecting cells in a cell population at a total particle to cell ratio of less than about 100:1 to about 0.1:1 to generate infected cells ; ii) reacting a polypeptide expressed by the virus in infected cells with an antibody labeled with a fluorescent tag, the antibody having specificity for a polypeptide expressed by the virus; and iii) measuring immunofluorescence in the product of step (ii) by flow cytometry to determine the number of infected cells, thereby determining the number of infectious virus particles.
- the viral polypeptide can be encoded by an exogenous gene, such as a reporter gene.
- the exogenous gene is a tumor suppressor gene such as p53 or retinoblastoma (RB) .
- the recombinant virus can be replication competent or defective, deficient or incompetent.
- the virus is adenovirus.
- the viral polypeptide can be an adenovirus polypeptide such as hexon .
- the viral polypeptide is reacted with at least one antibody, although the antibody can be a mixture of antibodies.
- the antibody can be polyclonal or monoclonal.
- the total particle to cell ratio is less than about 100:1, typically less than about 10:1, preferably less than about 5:1, more preferably less than about 1:1. In some embodiments, the ratio can be as low as about 0.1:1.
- the instant invention provided methods for quantitating infectious viral particles in a population of virus particles.
- infectious as used herein is intended to refer to the ability of a virus to enter cells and direct the synthesis of at least one polypeptide encoded by the virus .
- the ability to reproduce the viral nucleic acid is not required, but is included, by this definition.
- not every virus particle in a preparation is infectious.
- particles can be damaged in preparation of the virus, thereby not affecting total particle number but decreasing the number of particles capable of infection.
- empty capsids or instability of the virus extracellularly can also contribute to the decrease in infectivity.
- the range of non-infectious particles to infectious particles in viral preparations can range from 1:1 to greater than 100:1. However, even non-infectious viruses can cause cytological changes or damage to exposed cells.
- any virus can be quantitated, or titered, by the methods of the instant invention, including DNA viruses, RNA viruses, replication competent viruses, replication incompetent viruses, recombinant viruses, viruses carrying transgenes, etc.
- the virus can infect cells in culture.
- viruses amenable to this technique include, but are not limited to, adenovirus, adeno- associated virus, retrovirus, herpes simplex virus, parvovirus, Epstein Barr virus, rhinotracheitis virus, parainfluenza virus, bovine diarrhea virus, Sindbis virus, baculovirus, pseudorabies virus, varicella- zoster virus, cytomegalovirus, HIV, hepatitis A, B, and C viruses, and vaccinia .
- infectivity is measured by antibodies directed against a polypeptide expressed by the virus.
- the polypeptide may be a structural viral polypeptide, a regulatory polypeptide, a polypeptide such as a polymerase, and so on.
- the polypeptide is preferably expressed by an exogenous gene incorporated into the virus, such as a reporter gene.
- reporter genes include ⁇ -galactosidase and chloramphenicol transacetylase (CAT) .
- the reporter gene is detected by antibodies directed against a product of the action of the reporter gene, such as the action of an enzyme on a substrate.
- the exogenous gene is a transgene intended for therapeutic use.
- tumor suppressor genes including p53 or retinoblastoma (RB) ; interleukins, including I -2, IL-4, and IL-10; interferons, including alpha-, beta-, and gamma interferon; other cytokines; thymidine kinase; growth factors, including GCSF and growth hormone; Factor VIII; adenosine deaminase, and so on.
- tumor suppressor genes including p53 or retinoblastoma (RB) ; interleukins, including I -2, IL-4, and IL-10; interferons, including alpha-, beta-, and gamma interferon; other cytokines; thymidine kinase; growth factors, including GCSF and growth hormone; Factor VIII; adenosine deaminase, and so on.
- production of polypeptide encoded by a transgene will be measured by an antibody directed against the polypeptide.
- Antibodies used for detection can be polyclonal, monoclonal, or include mixtures of such antibodies. Typically, the detection is done directly by using a fluorescein-conjugated antibody directed against the viral polypeptide. However, indirect assays are also possible, in which the antibody directed against the viral polypeptide is then reacted with a fluorescein- labeled antibody. Any fluorescent label compatible with flow cytometry can be used.
- the total number of virus particles in a viral preparation is first measured by any of a number of traditional techniques.
- an aliquot of a virus preparation can be prepared in a buffer containing 0.1% sodium dodecyl sul ate (SDS) , after which the optical absorbance is measured at 260 nm (Maizel et al . Virology 36:115-125 (1968)).
- Total particle counts can also be obtained by preparing a sample of the viral preparation for electron microscopy, and simply counting the number of particles.
- a further technique for particle enumeration can include the use of anion-exchange chro atography (Huyghe et al. Human Gene Therapy 6:1403-1416 (1995) ) .
- Cells are then infected with dilutions of the viral preparations at total particle number to cell number ratios no higher than about 100:1, typically less than about 10:1, preferably less than about 5:1, more preferably less than about 1:1. In some embodiments the ratio is as low as about 0.1:1. Typically, at least one infection will be performed, although in some embodiments at least two parallel infections are performed at different particle to cell ratios.
- the cells used are typically known to be sensitive to infection by the virus. It is not required that the cells support replication by the virus, but the infection is performed under conditions that allow expression of the viral polypeptide to be detected.
- the total volume of a virus preparation used to infect cells in culture is typically determined by the skilled artisan by taking into account such factors as the total number of cells to be infected, the particle concentration of the virus preparation, and the volume of the vessel in which the infection is performed.
- the particle concentration of virus used to infect cells in the infection mixture is at least about 10 5 particles per ml, more preferably at least about 10 6 particles per ml, most preferably about 10 7 particles per ml.
- the viral preparations typically are prepared under conditions favorable to stability of the virus. Conditions for infection and, optionally, culture after infection will depend on the particular virus and the viral or reporter gene used for detection.
- culture refers to any form of cell culture in which the minimum requirements are provided to the cells to enable continued survival for the period of interest.
- culture can refer to preparation of a cell suspension in a suitable buffer, such as phosphate buffered saline or an incomplete growth medium, for a period of minutes or hours, or can refer cells adhering to culture dishes for minutes to days to weeks in the presence of a suitable complete growth medium.
- suitable buffer such as phosphate buffered saline or an incomplete growth medium
- sufficient time in culture is provided for expression of the desired viral polypeptide, but preferably not enough time is provided for propagation of the infecting virus which results in further infection of cells.
- the length of time allowed "in culture” will be less than 1 hour to several hours. In other preferred embodiments, the length of time will be 1 to 5 days.
- cells are infected under conditions favoring adsorption of the virus to the cells, although less optimal conditions can be used in some embodiments.
- viruses are allowed to adsorb to cells for 1-12 hours.
- the cells are infected in a concentrated suspension with concentrated virus, to enhance the rate of infection or the number of infected cells, then diluted to a concentration more favorable for cell or viral growth.
- it can be desirable to wash infected cells cultures to remove unabsorbed virus or components of the medium used for infection, or to expose the infected cells to media or growth conditions more favorable to their survival .
- the cells are typically prepared as a suspension of single cells.
- the cultures are typically treated with a dissociating agent such as trypsin to detach the cells from the substratum.
- a dissociating agent such as trypsin to detach the cells from the substratum.
- Mechanical means can also be used to detach cells, such as scraping.
- Cells are then collected by centrifugation and prepared in a buffer, such as incomplete or complete growth medium, for reaction with the detection reagents.
- a buffer such as incomplete or complete growth medium
- fluorescent reagents can be introduced into cells to allow detection of the activity, such as a fluorescein labeled substrate for an enzyme.
- Infected cell populations are then subjected to analysis by standard flow cytometry, such as by the methods disclosed by Shapiro, Practical Flow Cytometry. 3rd ed. , John Wiley and Sons (1994), hereby incorporated by reference in its entirety for all purposes.
- the term "FACS” is sometimes used to refer to flow cytometry, although cell sorting is not required to practice the instant invention.
- a minimum of about 10,000 events is acquired in the analysis.
- Dead cells are typically excluded from the analysis either by forward/side scatter gating or PI labelling and setting of electronic windows on the PI negative fraction.
- a variety of commercial software packages are available to aid in preparation and analysis of the data, such as CellQuestTM.
- ACNRB a recombinant, replicative- defective adenovirus was titered by TCID 50 and by the low particle number to cell number ratio (low ratio) method of the present invention.
- the exemplary virus used essentially comprised the adenovirus vector backbone disclosed by Wills et al . (Cancer Gene Therapy 2:191-197 (1995)) with full-length retinoblastoma cDNA inserted into the vector.
- Total particle number was obtained by the "SDS/OD 260 " method and anion exchange chromatography methods described above. In both assays the measured total particle concentration was 1.0 X 10 12 /ml.
- Infectious particles were titered by TCID b0 assay as described by Huyghe et al . (Human Gene Therapy 6:1403-1416 (1995)).
- 293 cells were plated into a 96-well microtiter plate: lOO ⁇ l of 5xl0 5 cells/ml for each well in complete MEM (10% bovine calf serum; 1% glutamine) media (GIBCO BRL) .
- MEM % bovine calf serum; 1% glutamine
- GIBCO BRL complete MEM (10% bovine calf serum; 1% glutamine) media
- a 250- ⁇ l aliquot of virus sample diluted 1:10 6 was added to the first column and was serially diluted two- fold across the plate. Seven rows were used for samples. One row was used for a negative control.
- the low ratio assay was performed as follows . 1 X 10 6 293 cells (human embryonic kidney cells, ATCC CRL 1573) were seeded per well on 4 6-well dishes. The final volume per well was 1 ml. After about 2 hr, the medium (Dulbecco's modified Eagle's medium (DME high glucose) containing 4500 g/ l D-glucose, supplemented with 5% defined, iron- supplemented bovine calf serum, 2mM L-glutamine, and 1 mM sodium pyruvate) in each well was aspirated and replaced with 1.1 ml of medium (without serum) containing diluted virus.
- DME high glucose Dulbecco's modified Eagle's medium
- Adsorption was allowed to occur for 60 minutes, after which an additional 2ml of virus-free medium was added to each well. After about 42 hr, the infected cells cultures were processed for flow cytometry analysis. The cells were detached from the plastic substratum with a trypsin-EDTA solution (GIBCO-BRL) . Detached cells were collected from each well and centrifuged at about 200 x g for 10 minutes at room temperature. The supernatants were removed and the cells washed in Dulbecco's phosphate buffered saline (D-PBS) without calcium or magnesium salts.
- D-PBS Dulbecco's phosphate buffered saline
Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP50533298A JP3851662B2 (en) | 1996-07-09 | 1997-07-07 | Methods for measuring virus infectivity |
CA002259549A CA2259549C (en) | 1996-07-09 | 1997-07-07 | Method for measuring viral infectivity |
AT97932541T ATE260990T1 (en) | 1996-07-09 | 1997-07-07 | METHOD FOR MEASURING VIRAL INFECTIVITY |
DE69727984T DE69727984T2 (en) | 1996-07-09 | 1997-07-07 | METHOD FOR MEASURING VIRAL INFECTIVITY |
AU35973/97A AU3597397A (en) | 1996-07-09 | 1997-07-07 | Method for measuring viral infectivity |
EP97932541A EP1023467B1 (en) | 1996-07-09 | 1997-07-07 | Method for measuring viral infectivity |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US67848596A | 1996-07-09 | 1996-07-09 | |
US08/678,485 | 1996-07-09 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1998001582A1 true WO1998001582A1 (en) | 1998-01-15 |
Family
ID=24722979
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1997/011865 WO1998001582A1 (en) | 1996-07-09 | 1997-07-07 | Method for measuring viral infectivity |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP1023467B1 (en) |
JP (1) | JP3851662B2 (en) |
AT (1) | ATE260990T1 (en) |
AU (1) | AU3597397A (en) |
DE (1) | DE69727984T2 (en) |
ES (1) | ES2216158T3 (en) |
WO (1) | WO1998001582A1 (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2077862A2 (en) * | 2006-11-03 | 2009-07-15 | Epeius Biotechnologies, Inc. | Pathotropic targeted gene delivery system for cancer and other disorders |
US8052966B2 (en) | 2003-04-21 | 2011-11-08 | University Of Southern California | Methods and compositions for treating metastatic cancer |
US8828378B2 (en) | 2000-11-29 | 2014-09-09 | The University Of Southern California | Targeted vectors for cancer immunotherapy |
WO2017019404A1 (en) * | 2015-07-24 | 2017-02-02 | Merial, Inc. | High throughput methods for virus quantification |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5470730A (en) * | 1990-09-28 | 1995-11-28 | Immunex | Method for producing TH -independent cytotoxic T lymphocytes |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5733781A (en) * | 1994-07-19 | 1998-03-31 | Gen-Probe Incorporated | Oligonucleotides and methods for inhibiting propagation of human immunodeficiency virus |
-
1997
- 1997-07-07 JP JP50533298A patent/JP3851662B2/en not_active Expired - Fee Related
- 1997-07-07 ES ES97932541T patent/ES2216158T3/en not_active Expired - Lifetime
- 1997-07-07 DE DE69727984T patent/DE69727984T2/en not_active Expired - Fee Related
- 1997-07-07 EP EP97932541A patent/EP1023467B1/en not_active Expired - Lifetime
- 1997-07-07 AT AT97932541T patent/ATE260990T1/en not_active IP Right Cessation
- 1997-07-07 WO PCT/US1997/011865 patent/WO1998001582A1/en active IP Right Grant
- 1997-07-07 AU AU35973/97A patent/AU3597397A/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5470730A (en) * | 1990-09-28 | 1995-11-28 | Immunex | Method for producing TH -independent cytotoxic T lymphocytes |
Non-Patent Citations (3)
Title |
---|
EYLER Y L, LANTZ L M, LEWIS A M: "FLOW CYTOMETRIC DETECTION OF DNA TUMOR VIRUS NUCLEAR ONCOGENE PRODUCTS IN UNFIXED CELLS: SAPONIN FACS OF VIRAL ONCOGENE PRODUCTS", JOURNAL OF VIROLOGICAL METHODS, ELSEVIER BV, NL, vol. 46, 1 January 1994 (1994-01-01), NL, pages 23 - 27, XP002909836, ISSN: 0166-0934, DOI: 10.1016/0166-0934(94)90013-2 * |
SAALMUELLER A, METTENLEITER T C: "RAPID IDENTIFICATION AND QUANTITATION OF CELLS INFECTED BY RECOMBINANT HERPESVIRUS (PSEUDORABIES VIRUS) USING A FLUORESCENCE-BASED BETA-GALACTOSIDASE ASSAY AND FLOW CYTOMETRY", JOURNAL OF VIROLOGICAL METHODS, ELSEVIER BV, NL, vol. 44, 1 January 1993 (1993-01-01), NL, pages 99 - 108, XP002909835, ISSN: 0166-0934, DOI: 10.1016/0166-0934(93)90012-G * |
SHU FEN WEN, ET AL.: "RETINOBLASTOMA PROTEIN MONOCLONAL ANTIBODIES WITH NOVEL CHARACTERISTICS", JOURNAL OF IMMUNOLOGICAL METHODS., ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM., NL, vol. 169, 1 January 1994 (1994-01-01), NL, pages 231 - 240, XP002909826, ISSN: 0022-1759, DOI: 10.1016/0022-1759(94)90267-4 * |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8828378B2 (en) | 2000-11-29 | 2014-09-09 | The University Of Southern California | Targeted vectors for cancer immunotherapy |
US8052966B2 (en) | 2003-04-21 | 2011-11-08 | University Of Southern California | Methods and compositions for treating metastatic cancer |
EP2077862A2 (en) * | 2006-11-03 | 2009-07-15 | Epeius Biotechnologies, Inc. | Pathotropic targeted gene delivery system for cancer and other disorders |
EP2077862A4 (en) * | 2006-11-03 | 2011-03-09 | Epeius Biotechnologies Corp | Pathotropic targeted gene delivery system for cancer and other disorders |
US9017659B2 (en) | 2006-11-03 | 2015-04-28 | Epeius Biotechnologies Corporation | Pathotropic targeted gene delivery system for cancer and other disorders |
WO2017019404A1 (en) * | 2015-07-24 | 2017-02-02 | Merial, Inc. | High throughput methods for virus quantification |
US10663468B2 (en) | 2015-07-24 | 2020-05-26 | Boehringer Ingelheim Animal Health USA Inc. | High throughput methods for virus quantification |
Also Published As
Publication number | Publication date |
---|---|
EP1023467A4 (en) | 2002-02-13 |
DE69727984T2 (en) | 2005-01-05 |
EP1023467A1 (en) | 2000-08-02 |
AU3597397A (en) | 1998-02-02 |
DE69727984D1 (en) | 2004-04-08 |
ES2216158T3 (en) | 2004-10-16 |
ATE260990T1 (en) | 2004-03-15 |
EP1023467B1 (en) | 2004-03-03 |
JP3851662B2 (en) | 2006-11-29 |
JP2000514922A (en) | 2000-11-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US6248514B1 (en) | Methods for measuring viral infectivity | |
Temin | The participation of DNA in Rofs sarcoma virus production | |
Ozawa et al. | Productive infection by B19 parvovirus of human erythroid bone marrow cells in vitro | |
Blair et al. | Restricted replication of human adenovirus type 5 in mouse cell lines | |
Winocour et al. | Cell-virus interactions with the polyoma virus: I. Studies on the lytic interaction in the mouse embryo system | |
Broder et al. | Expression of foreign genes in cultured human primary macrophages using recombinant vaccinia virus vectors | |
Strauss et al. | Mutants of Sindbis virus: I. Isolation and partial characterization of 89 new temperature-sensitive mutants | |
Duus et al. | Wild-type Kaposi's sarcoma-associated herpesvirus isolated from the oropharynx of immune-competent individuals has tropism for cultured oral epithelial cells | |
Aurelian et al. | Abortive infection of canine cells by herpes simplex virus: II. Alternative suppression of synthesis of interferon and viral constituents | |
Cepko et al. | Analysis of Ad5 Hexon and 100K is mutants using conformation-specific monoclonal antibodies | |
Laroche et al. | Measurement by the polymerase chain reaction of the Epstein‐Barr virus load in infectious mononucleosis and AIDS‐related non‐Hodgkin's lymphomas | |
Rhode 3rd | Replication process of the parvovirus H-1 V. Isolation and characterization of temperature-sensitive H-1 mutants | |
Gueret et al. | Rapid titration of adenoviral infectivity by flow cytometry in batch culture of infected HEK293 cells | |
EP1023467B1 (en) | Method for measuring viral infectivity | |
Finnen et al. | Truncation of the human adenovirus type 5 L4 33-kDa protein: evidence for an essential role of the carboxy-terminus in the viral infectious cycle | |
Carpenter et al. | Egress of light particles among filopodia on the surface of varicella-zoster virus-infected cells | |
Weaver et al. | Evaluation of adenoviral vectors by flow cytometry | |
Shiroki et al. | Analysis of adenovirus 12 temperature-sensitive mutants defective in viral DNA replication | |
Field et al. | Detection of B19 parvovirus in human fetal tissues by electron microscopy | |
CA2259549C (en) | Method for measuring viral infectivity | |
CA2531850A1 (en) | Method for measuring viral infectivity | |
MXPA99000396A (en) | Method for measuring inefficiency vi | |
LaRocco | Evaluation of an enzyme-linked viral inducible system for the rapid detection of herpes simplex virus | |
Park et al. | Rapid titer assay of adenovirus containing green fluorescent protein gene using flow cytometric analysis | |
Gleaves et al. | Detection of human cytomegalovirus in clinical specimens by centrifugation culture with a nonhuman cell line |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG US UZ VN YU ZW AM AZ BY KG KZ MD RU TJ TM |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH KE LS MW SD SZ UG ZW AT BE CH DE DK ES FI FR GB GR IE IT LU MC NL |
|
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
ENP | Entry into the national phase |
Ref document number: 2259549 Country of ref document: CA Ref country code: CA Ref document number: 2259549 Kind code of ref document: A Format of ref document f/p: F |
|
WWE | Wipo information: entry into national phase |
Ref document number: PA/a/1999/000396 Country of ref document: MX |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1997932541 Country of ref document: EP |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWP | Wipo information: published in national office |
Ref document number: 1997932541 Country of ref document: EP |
|
WWG | Wipo information: grant in national office |
Ref document number: 1997932541 Country of ref document: EP |