WO1997032204A1 - Protein separations by capillary electrophoresis using amino acid-containing buffers - Google Patents
Protein separations by capillary electrophoresis using amino acid-containing buffers Download PDFInfo
- Publication number
- WO1997032204A1 WO1997032204A1 PCT/US1997/002179 US9702179W WO9732204A1 WO 1997032204 A1 WO1997032204 A1 WO 1997032204A1 US 9702179 W US9702179 W US 9702179W WO 9732204 A1 WO9732204 A1 WO 9732204A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- capillary
- buffer
- accordance
- amino acid
- electrophoresis
- Prior art date
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP97907607A EP0832428A1 (en) | 1996-02-29 | 1997-02-26 | Protein separations by capillary electrophoresis using amino acid-containing buffers |
AU19570/97A AU701982B2 (en) | 1996-02-29 | 1997-02-26 | Protein separations by capillary electrophoresis using amino acid-containing buffers |
JP9521519A JPH10507004A (en) | 1996-02-29 | 1997-02-26 | Separation of proteins by capillary electrophoresis using amino acid-containing buffer |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/610,105 | 1996-02-29 | ||
US08/610,105 US5660701A (en) | 1996-02-29 | 1996-02-29 | Protein separations by capillary electrophoresis using amino acid-containing buffers |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1997032204A1 true WO1997032204A1 (en) | 1997-09-04 |
Family
ID=24443677
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1997/002179 WO1997032204A1 (en) | 1996-02-29 | 1997-02-26 | Protein separations by capillary electrophoresis using amino acid-containing buffers |
Country Status (7)
Country | Link |
---|---|
US (2) | US5660701A (en) |
EP (1) | EP0832428A1 (en) |
JP (1) | JPH10507004A (en) |
CN (1) | CN1185836A (en) |
AU (1) | AU701982B2 (en) |
CA (1) | CA2207541A1 (en) |
WO (1) | WO1997032204A1 (en) |
Families Citing this family (36)
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US6017696A (en) | 1993-11-01 | 2000-01-25 | Nanogen, Inc. | Methods for electronic stringency control for molecular biological analysis and diagnostics |
US5605662A (en) | 1993-11-01 | 1997-02-25 | Nanogen, Inc. | Active programmable electronic devices for molecular biological analysis and diagnostics |
US6051380A (en) * | 1993-11-01 | 2000-04-18 | Nanogen, Inc. | Methods and procedures for molecular biological analysis and diagnostics |
US5849486A (en) | 1993-11-01 | 1998-12-15 | Nanogen, Inc. | Methods for hybridization analysis utilizing electrically controlled hybridization |
US6331274B1 (en) | 1993-11-01 | 2001-12-18 | Nanogen, Inc. | Advanced active circuits and devices for molecular biological analysis and diagnostics |
US20040077074A1 (en) * | 1993-11-01 | 2004-04-22 | Nanogen, Inc. | Multi-chambered analysis device |
US7172864B1 (en) | 1993-11-01 | 2007-02-06 | Nanogen | Methods for electronically-controlled enzymatic reactions |
US6225059B1 (en) | 1993-11-01 | 2001-05-01 | Nanogen, Inc. | Advanced active electronic devices including collection electrodes for molecular biological analysis and diagnostics |
US7582421B2 (en) * | 1993-11-01 | 2009-09-01 | Nanogen, Inc. | Methods for determination of single nucleic acid polymorphisms using a bioelectronic microchip |
US7314708B1 (en) * | 1998-08-04 | 2008-01-01 | Nanogen, Inc. | Method and apparatus for electronic synthesis of molecular structures |
US7101661B1 (en) | 1993-11-01 | 2006-09-05 | Nanogen, Inc. | Apparatus for active programmable matrix devices |
US6309602B1 (en) | 1993-11-01 | 2001-10-30 | Nanogen, Inc. | Stacked, reconfigurable system for electrophoretic transport of charged materials |
GB9322648D0 (en) | 1993-11-03 | 1993-12-22 | Ass Octel | Process for the production of s.s.e.d.d.s |
US6379897B1 (en) * | 2000-11-09 | 2002-04-30 | Nanogen, Inc. | Methods for gene expression monitoring on electronic microarrays |
US7857957B2 (en) | 1994-07-07 | 2010-12-28 | Gamida For Life B.V. | Integrated portable biological detection system |
US6403367B1 (en) | 1994-07-07 | 2002-06-11 | Nanogen, Inc. | Integrated portable biological detection system |
US20040086917A1 (en) * | 1995-09-27 | 2004-05-06 | Nanogen, Inc. | Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis |
US5964995A (en) * | 1997-04-04 | 1999-10-12 | Caliper Technologies Corp. | Methods and systems for enhanced fluid transport |
DE19741715A1 (en) * | 1997-09-22 | 1999-03-25 | Hoechst Ag | New pentopyranosyl nucleoside compounds |
US6156179A (en) * | 1998-07-09 | 2000-12-05 | Bio-Rad Laboratories | Computer directed identification of paraproteins |
US6475364B1 (en) * | 1999-02-02 | 2002-11-05 | Caliper Technologies Corp. | Methods, devices and systems for characterizing proteins |
US20050027111A1 (en) * | 1999-02-02 | 2005-02-03 | Caliper Life Sciences, Inc. | Methods, devices and systems for characterizing proteins |
FR2819888B1 (en) * | 2001-01-19 | 2003-09-12 | Sebia Sa | PROCESS FOR SEPARATING PROTEINS BY CAPILLARY ELECTROPHORESIS AND BUFFER COMPOSITIONS FOR CAPILLARY ELECTROPHORESIS |
FR2819889B1 (en) * | 2001-01-19 | 2003-08-01 | Sebia Sa | METHOD OF CAPILLARY ELECTROPHORESIS IN FREE ALKALINE PH SOLUTION |
US7601493B2 (en) * | 2002-07-26 | 2009-10-13 | Nanogen, Inc. | Methods and apparatus for screening and detecting multiple genetic mutations |
US7645293B2 (en) | 2004-04-21 | 2010-01-12 | United States Surgical Corporation | Suture anchor installation system and method |
CN100337113C (en) * | 2004-04-30 | 2007-09-12 | 首都师范大学 | Acidic capillary electrophoresis identification method for high molecular weight glutelin subunit of wheat |
FR2869995B1 (en) * | 2004-05-10 | 2006-09-22 | Sebia Sa | IMPROVED CAPILLARY ELECTROPHORESIS PROTEIN SEPARATION PROCESS AND CAPILLARY ELECTROPHORESIS BUFFER COMPOSITIONS |
US7314542B2 (en) * | 2004-09-23 | 2008-01-01 | Nanogen, Inc. | Methods and materials for optimization of electronic transportation and hybridization reactions |
KR100978055B1 (en) * | 2008-08-25 | 2010-08-25 | 한국과학기술연구원 | Method for enhancing the detection sensitivity of capillary electrophoresis using improved sample injection mode |
CN101915696B (en) * | 2010-07-07 | 2014-08-13 | 邯郸开发区奥泰克生物科技有限公司 | Method for preparing capillary inner wall coating |
US9804079B2 (en) | 2012-04-19 | 2017-10-31 | ProteinSimple | Dual wavelength isoelectric focusing for determining drug load in antibody drug conjugates |
US20150093757A1 (en) * | 2013-09-27 | 2015-04-02 | Protein Simple | Methods. apparatus and systems for detection of total protein using capillary electrophoresis |
EP3402894A1 (en) * | 2016-01-15 | 2018-11-21 | Pierce Biotechnology, Inc. | Protein preparation by reduction of oxidized methionines |
WO2021154854A1 (en) * | 2020-01-27 | 2021-08-05 | Bristol-Myers Squibb Company | Isoelectric focusing sample matrix |
CN114544738A (en) * | 2022-02-15 | 2022-05-27 | 河北北方学院 | Electrophoresis buffer solution |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1106818A (en) * | 1964-08-26 | 1968-03-20 | Lkb Produkter Ab | Mixture of carrier ampholytes and method of preparation thereof |
DE2656801A1 (en) * | 1976-12-15 | 1978-06-22 | Max Planck Gesellschaft | PROCESS FOR THE PRODUCTION OF CARRAMPHOLYTES FOR THE ISOELECTRIC FOCUSING OF HIGH MOLECULAR AMPHOTERIC SUBSTANCES |
DE2809977A1 (en) * | 1978-03-08 | 1979-09-13 | Serva Entwicklungslabor V Grot | Di:amino-mono:- and poly:carboxylic acid mixt. - prepd. from di:amine mixt. and acrylic acid homologue mixt. and used as isoelectric focussing carrier ampholyte |
EP0029284A1 (en) * | 1979-11-07 | 1981-05-27 | Gelman Sciences, Inc. | Method for the electrophoretic separation of proteins, a buffer solution and buffer compositions therefor |
EP0292837A1 (en) * | 1987-05-27 | 1988-11-30 | Abbott Laboratories | Polycationic buffers and method for gel electrophoresis of nucleic acids |
WO1989000689A1 (en) * | 1987-07-17 | 1989-01-26 | Beckman Instruments, Inc. | High resolution electrophoretic gel and method for separating serum proteins |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5221447A (en) * | 1991-12-06 | 1993-06-22 | Bio-Rad Laboratories, Inc. | Hydrophilic polymer coating of high pH stability for silica surfaces for suppression of electroendomosis and solute adsorption |
-
1996
- 1996-02-29 US US08/610,105 patent/US5660701A/en not_active Ceased
-
1997
- 1997-02-26 CN CN97190003A patent/CN1185836A/en active Pending
- 1997-02-26 CA CA002207541A patent/CA2207541A1/en not_active Abandoned
- 1997-02-26 JP JP9521519A patent/JPH10507004A/en active Pending
- 1997-02-26 EP EP97907607A patent/EP0832428A1/en not_active Withdrawn
- 1997-02-26 WO PCT/US1997/002179 patent/WO1997032204A1/en not_active Application Discontinuation
- 1997-02-26 AU AU19570/97A patent/AU701982B2/en not_active Ceased
- 1997-11-20 US US08/975,179 patent/USRE36011E/en not_active Expired - Lifetime
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1106818A (en) * | 1964-08-26 | 1968-03-20 | Lkb Produkter Ab | Mixture of carrier ampholytes and method of preparation thereof |
DE2656801A1 (en) * | 1976-12-15 | 1978-06-22 | Max Planck Gesellschaft | PROCESS FOR THE PRODUCTION OF CARRAMPHOLYTES FOR THE ISOELECTRIC FOCUSING OF HIGH MOLECULAR AMPHOTERIC SUBSTANCES |
DE2809977A1 (en) * | 1978-03-08 | 1979-09-13 | Serva Entwicklungslabor V Grot | Di:amino-mono:- and poly:carboxylic acid mixt. - prepd. from di:amine mixt. and acrylic acid homologue mixt. and used as isoelectric focussing carrier ampholyte |
EP0029284A1 (en) * | 1979-11-07 | 1981-05-27 | Gelman Sciences, Inc. | Method for the electrophoretic separation of proteins, a buffer solution and buffer compositions therefor |
EP0292837A1 (en) * | 1987-05-27 | 1988-11-30 | Abbott Laboratories | Polycationic buffers and method for gel electrophoresis of nucleic acids |
WO1989000689A1 (en) * | 1987-07-17 | 1989-01-26 | Beckman Instruments, Inc. | High resolution electrophoretic gel and method for separating serum proteins |
Non-Patent Citations (1)
Title |
---|
N. COHEN: "CONTROLLING ELECTROOSMOTIC FLOW IN CAPILLARY ZONE ELECTROPHORESIS", JOURNAL OF CHROMATOGRAPHY A, vol. 678, no. 1, 1994, pages 167 - 175, XP000674000 * |
Also Published As
Publication number | Publication date |
---|---|
AU701982B2 (en) | 1999-02-11 |
JPH10507004A (en) | 1998-07-07 |
US5660701A (en) | 1997-08-26 |
CA2207541A1 (en) | 1997-08-29 |
AU1957097A (en) | 1997-09-16 |
EP0832428A1 (en) | 1998-04-01 |
CN1185836A (en) | 1998-06-24 |
USRE36011E (en) | 1998-12-29 |
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