WO1997031024A1 - Antibody variants - Google Patents

Antibody variants Download PDF

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Publication number
WO1997031024A1
WO1997031024A1 PCT/GB1997/000472 GB9700472W WO9731024A1 WO 1997031024 A1 WO1997031024 A1 WO 1997031024A1 GB 9700472 W GB9700472 W GB 9700472W WO 9731024 A1 WO9731024 A1 WO 9731024A1
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WIPO (PCT)
Prior art keywords
antibody
therapeutic
fragment
binding
cell
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PCT/GB1997/000472
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English (en)
French (fr)
Inventor
Herman Waldmann
Lisa Kim Gilliland
Masahide Tone
Mark Raymond Frewin
Louise Walsh
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Isis Innovation Limited
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Application filed by Isis Innovation Limited filed Critical Isis Innovation Limited
Priority to EP97905226A priority Critical patent/EP0970127B1/en
Priority to NZ331299A priority patent/NZ331299A/xx
Priority to JP52989397A priority patent/JP4574750B2/ja
Priority to AU18851/97A priority patent/AU723366B2/en
Priority to DE69738138T priority patent/DE69738138T2/de
Priority to CA002246715A priority patent/CA2246715C/en
Publication of WO1997031024A1 publication Critical patent/WO1997031024A1/en
Priority to US11/180,631 priority patent/US8440190B2/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/461—Igs containing Ig-regions, -domains or -residues form different species
    • C07K16/464—Igs containing CDR-residues from one specie grafted between FR-residues from another
    • C07K16/465—Igs containing CDR-residues from one specie grafted between FR-residues from another with additional modified FR-residues
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2893—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD52
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Definitions

  • This invention relates to modified antibodies for inducing immunological tolerance in human beings or animals.
  • Antibodies, or immunoglobulins comprise two heavy chains linked together by disulphide bonds and two light chains, each light chain being linked to a respective heavy chain by disulphide bonds.
  • Each heavy chain has at one end a variable domain followed by a number of constant domains.
  • Each light chain has a variable domain at one end and a constant domain at its other end, the light chain variable domain being aligned with the variable domain of the heavy chain and the light chain constant domain being aligned with the first constant domain of the heavy chain.
  • the constant domains in the light and heavy chains are not involved directly in binding the antibody to antigen.
  • Antibodies directed to specifically chosen antigens have been used in the treatment of various conditions.
  • Campath-1 monoclonal antibodies have been used successfully to induce remissions in lymphoma and leukemia patients and for the treatment of rheumatoid arthritis and vasculitis.
  • the target antigen, CD52 also referred to as CDw52; see e.g. Xia et al., 1991
  • CD52 is a GPI-anchored glycoprotein of lymphocytes and monocytes (and parts of the male reproductive system). It has an exceptionally short peptide sequence of 12 amino acids and a single, complex, N-linked oligosaccharide at Asn3 (Hale et al, 1990; Xia et al, 1991).
  • CD52 is a good target for antibody-mediated killing and is therefore an effective cell surface molecule for various therapeutic regimens in which reduction in lymphocytes is an objective (e.g. removal of cells from donor bone marrow to prevent graft-versus-host disease, treatment of leukemia and lymphoma, and immuno-suppression).
  • Several rat anti-human CD52 Campath-1 mAb were generated by fusion of the Y3 rat myeloma line with spleen cells from a rat immunized with human T lymphocytes (Hale et al, 1983).
  • anti-antibody antiglobulin
  • Antibody therapy is often limited by the antiglobulin response.
  • the anti-idiotypic component (anti-Id; directed against the Ab V regions and in particular the Ab-combining site) inhibits the binding of the Ab to its target while both the anti-Id and the anti-isotypic component (directed against the constant regions) act to accelerate antibody clearance.
  • anti-Id directed against the Ab V regions and in particular the Ab-combining site
  • a major concern is the neutralizing effect of the antiglobulin response.
  • anti-Id responses interfere with the clinical potency of a therapeutic Ab by forming Ab aggregates that are rapidly cleared from the circulation, reducing the chance for interaction with target antigen.
  • most antiglobulin sera contain anti-Id antibodies. This has been demonstrated for a number of therapeutic mAb and is especially noted after repeated treatments.
  • the gene fragments encoding the VL and VH were humanized by "CDR grafting" of the rodent hypervariable regions onto human framework regions (Jones et al, 1986; Reichmann et al, 1988). This was carried out by splicing the CDR sequences encoding the rat Campath-1 antibody onto sequence encoding human framework backbone provided by the crystallographically solved myeloma proteins NEW (for the VH) and REI (for the VL). The resulting protein had low antigen-binding titre and modelling of the humanized V-region showed that residue 27 in the VH framework sequence was critical for preserving the loop structure of CDR1.
  • the humanized Campath-1 antibody consists of human residues at ail positions except those encoding the 3 CDRs of the light chain, the 3 CDRs of the heavy chain, and residues Phe27 and Thr30 in VH of the heavy chain.
  • One strategy to further reduce the immunogenicity of Campath-1 H might be to re-graft the 6 CDR loops onto well-characterized human germline framework regions.
  • the majority of the humanized V regions so far have used rearranged V-genes as acceptor framework sequence. This was the case for Campath-1 H as framework sequences from myeloma proteins were used to provide acceptor sequences for both VH and VL.
  • Rearranged V-genes often contain somatic mutations, acquired during the process of affinity maturation. These will be unique to the individual from which the rearranged genes were derived and therefore may be seen as foreign in another individual. However, there is a possibility that regrafting may introduce new idiotypic epitopes, formed by the junctional regions encompassing CDR residues and new framework residues.
  • Another approach is to induce tolerance to the potentially foreign peptides contained within the Campath-1 H V-region.
  • the antiglobulin response is itself a B-cell response which is CD4+ T-cell dependent.
  • Isaacs and Waldmann (1994) demonstrated that mice deprived of CD4+ T-cells were unable to respond to a foreign cell-binding mAb (rat anti-mouse CD8 mAb).
  • CD4+ T-cell depletion was carried out by adult thymectomy combined with administration of a depleting CD4 mAb. In these mice, the response to subsequently administered mAb or SRBC was measured.
  • CD4+ T-cell deficient mice failed to make either an antiglobulin response or an anti-SRBC response, demonstrating that the anti-lg response, like the anti-SRBC response, is classically CD4+ T-cell dependent.
  • the adminstered Ab In order to generate T-cell help and to get the appropriate T- cell response, the adminstered Ab must be processed as a protein antigen and presented, presumably in the context of an MHC class II molecule, by a suitable antigen presenting cell. Therefore, two main strategies can be adopted to decrease the immunogenicity of a humanized V-region.
  • T helper peptides are not random, and any protein has only a limited number of peptides capable of binding to MHC class II molecules, and also to T-cell antigen receptors. However, this is not possible at present because class ll-binding peptides are not yet characterized to a sufficient degree to be identified by scanning protein sequences. This is in part due to the heterogeneous nature of class II peptides.
  • class II peptides are bound in an extended conformation that projects from both ends of an "open-ended” antigen-binding groove; a prominent non-polar pocket into which an anchoring peptide side chain fits near one end of the binding groove (Brown et al, 1993).
  • Other strategies that might be adopted to "silence” the antibody if we could predict class II peptide-binding motifs. For example, one could include insertion of a protease cleavage site within any potential class II epitope to increase the chance of peptide degredation before they could be presented in the context of class II.
  • insertion of motifs into a V-region may inhibit the degradation of the V-region into peptides that could associate with class II molecules.
  • EBNA1 Gly-Ala repeats generated a cis- acting inhibitory signal that interfered with antigen processing during MHC class l-restricted presentation such that CTL recognition was inhibited (Levitskaya et al, 1995).
  • the cell-binding antibody was an anti-CD8 mAb in a mouse model.
  • the non-cell-binding derivatives were made by pairing the relevant L- and H-chains with an irrelevant H- or L-chain, respectively.
  • the relevant H-chain paired with an irrelevant L-chain was obtained by limiting dilution cloning of the original hybridoma that was expressing a myeloma light chain (from the Y3 fusion partner), as well as the specific anti-CD8 H- and L-chains.
  • a variant of the hybridoma that expressed the myeloma L-chain and the specific anti-CD8 H-chain but no anti-CD8 L-chain was obtained.
  • a clone expressing the relevant L-chain only was also obtained in this manner. That clone was then fused to a hybridoma expressing an irrelevant specificity (anti-human CD3) and a variant was selected that expressed the relevant anti-CD8 L-chain with the irrelevant anti-CD3 H-chain. Because proteins are processed into peptides prior to presentation to T-cells, helper peptides from antigen-specific H- and L-chains would be "seen" by T-cells, regardless of their partner chain.
  • the affinity of the antibody according to the invention for the antigen is reduced to 50% or less of the affinity of the therapeutic antibody for the antigen. More preferably, the affinity is reduced to 10% or less, or to 1% or less of the affinity of the therapeutic antibody.
  • the affinity needs to be sufficiently reduced to allow the antibody according to the invention to act as a tolerogen with respect to the therapeutic antibody.
  • non-cell-binding variant is used herein to refer to antibodies according to the invention, although antibodies according to the invention may still have some binding affinity for the cell surface antigen.
  • the ability of the antibody according to the invention to induce immunological tolerance to a therapeutic cell-binding antibody relies on the presence in the non-cell-binding antibody of at least one epitope also present in the therapeutic antibody, which induces an immune response in the intended patient.
  • the non-cell-binding antibody is preferably capable of tolerising to anti-idiotypic responses, at least to the V domain hypervariable regions of the therapeutic antibody and preferably also to the framework regions.
  • the tolerising antibody has an amino acid sequence similar to the therapeutic antibody in those regions.
  • the differences are restricted to any amino acid substitution(s) required to sufficiently reduce antigen binding affinity in the non-cell-binding antibody.
  • the non-cell-binding antibody is capable of inducing tolerance to the constant regions of the therapeutic antibody.
  • the constant domains of the non-cell-binding antibody are similar to those of the therapeutic antibody, having for example >90% or >95% or >99% amino acid sequence identity.
  • the constant domains of the non-cell-binding antibody and the therapeutic antibody are identical and are thus matched allotypically.
  • the invention further provides fragments of an antibody described herein, the fragments having tolerance-inducing capability. Such fragments include monovalent and divalent fragments such as Fab, Fab- and F(ab') 2 fragments. Also included are single chain antibodies. The preferred features of such fragments are as described herein in relation to non-cell-binding antibodies according to the invention.
  • the non- cell-binding fragments may be for use with corresponding therapeutic antibody fragments, or with therapeutic antibody molecules.
  • the reduced binding affinity of the non-cell-binding antibodies may be achieved in a variety of ways.
  • an alteration in the CDRs comprising one or two or more amino acid substitutions reduces binding affinity.
  • amino acid substitutions in other parts of the antibody molecule may be used to reduce binding affinity.
  • amino acid substitutions in the framework regions are known to significantly affect binding affinity (Reichmann et al 1988;.
  • Another alternative is a monovalent form of the therapeutic antibody.
  • Monovalent antibodies have reduced binding affinity compared to their bivalent counterparts.
  • Monovalent forms may be for example Fab fragments, or single chain antibodies, or any other genetically engineered antibody fragments retaining a single binding site.
  • Monovalent variants can also be produced by mutating the cysteine residue which participates in interchain (H-H) disulphide formation (e.g., cys -+ ser or cys -*ala).
  • H-H interchain
  • the monovalent antibody is either incapable of binding Fc receptors, or incapable of binding complement component C1q, or both.
  • Either or both of these properties can be introduced by suitable mutations (see e.g., Morgan et al., WO 94/29351 , published 22 December 1994 and Winter et a/.,EP 0 307434 B1).
  • the non-cell-binding antibodies or fragments according to the invention may thus be one of a variety of types of antibodies or fragments, including genetically engineered antibodies or antibody fragments.
  • the antibodies or fragments will generally be from a mixture of origins.
  • they may be chimeric e.g. human constant regions with rat variable domains; or humanised or CDR grafted or otherwise reshaped (see, e.g., Cabilly et al., U.S. Patent No. 4,816,567; Cabilly et al., European Patent No. 0 125 023 B1; Boss et al., U.S. Patent No.
  • Campath-1H is considered humanised although it contains two amino acid substitutions in the framework regions.
  • non-cell-binding variant which contains the minimum differences needed to reduce its affinity for the cell- surface antigen sufficiently to enable it to be used as a tolerogen.
  • techniques such as X-ray crystallography, computer modeling and site- directed mutagenesis, and also genetic methods such as phage display, it will be possible to design suitable non-cell-binding variants for any cell- binding therapeutic antibody.
  • the antibody according to the invention is preferably in biologically pure form, desirably being at least 95% (by weight) free of other biological materials.
  • cell-surface antigen means an antigen which is found on cell surfaces, but not necessarily exclusively on cell surfaces.
  • therapeutic antibody is used herein to refer to an antibody which may be administered to humans or animals to have a desired effect in particular in the treatment of disease.
  • therapeutic antibodies will generally be monoclonal antibodies and will generally have been genetically engineered.
  • the invention comprises a composition for administration to a patient comprising an antibody as described herein, together with a physiologically acceptable diluent or carrier.
  • the invention provides a host cell or cell line which expresses an antibody as herein described and use of such a host cell or cell line for the production of such an antibody.
  • Figure 2 shows the PCR mutagenesis strategy for preparing the mutant constructs of Figure 1.
  • Figure 3 shows pGEM9zf containing wild type Campath-1H heavy chain, and substitution of mutant fragments in the heavy chain.
  • CDR sequences often fall into canonical loop motifs (excluding H3, between 50 and 95 % of murine VL (kappa) and VH have loop sequences consistent with classified canonical motifs).
  • Canonical loop backbones can then be spliced onto the model of the framework and CDR side-chain conformations can be modelled based on conformations of residues found at corresponding positions in other loops of the same canonical structure.
  • the model is often refined using computer programs that minimize troublesome stereochemical constraints.
  • VH CDR2 contained a key residue(s) necessary for antigen-binding.
  • DNA encoding the "wild-type" humanized Campath-1 H-chain was used as PCR template for site-directed mutagenesis. This heavy chain sequence encodes human protein at all positions except the three VH CDR regions and positions 27 and 30 of the first framework region.
  • Mutation 1 is a single charge difference at residue 52b from Lys to Asp. It was predicted from the molecular modelling of Campath-1 H Ab, and supported by the crystal structure, that the side chain of this residue is pointing out of the Ag binding pocket, towards the approach of antigen. Since the positive charge of the Lys is thought to interact with the negatively charged phosphate groups of the GPI anchor of CD52, it is possible that this single mutation will destroy antigen-binding.
  • Mutation 2 is a single charge difference at residue 52a from Asp to Lys. It was predicted from the molecular modelling of Campath-1 H Ab that this change could interfere with antigen-binding.
  • Mutation 3 is a single charge difference at residue 53 from Lys to Asp. From the crystal structure of Campath-1 H Ab, it is clear that the majority of this residue side chain is solvent accessible and therefore may be involved in the interaction with the negatively charged phosphate groups of the GPI anchor of CD52, as for mutation 1.
  • Mutation 4 is a double mutation encompassing the individual substitutions of mutant 1 and 3 (Lys52b and Lys53 to Asp).
  • PCR was carried out on "wild-type" Campath-1 heavy chain DNA using a 5' primer annealing to the leader sequence and containing an upstream Hindlll site (primer 1A) and primer 1 B to generate a 200 bp fragment.
  • primer 1A upstream Hindlll site
  • primer 2B annealing to CH1 and containing a BstXl site followed by an EcoRI site
  • primer 2A a 440 bp fragment was generated. These fragments were gel purified and then combined in a single PCR reaction.
  • Primer 1A and primer 2B were added after the first cycle (thus allowing the 2 pieces of overlapping DNA to anneal before amplification).
  • Campath-1H light chain DNA and mutant heavy chain DNA The expression system used to produce high levels of humanized Campath-1H Ab in the past is the widely used mammalian expression system featuring gene amplification by the use of dihydrofolate reductase (dhfr) deficient Chinese hamster ovary (CHO) cells and the use of strong ⁇ -actin promoters for selection and amplification of the desired gene products (Page and Sydenham, 1991).
  • dhfr dihydrofolate reductase
  • CHO Chinese hamster ovary
  • TF2 Light chain only (Light-chain/pRDN-1 plus "empty” pBAN-2)
  • TF9 Light chain/pRDN-1 plus H chain "wild-type"/pBAN-2 DNA (20 ⁇ g of light chain/pRDN-1 plus 20 ⁇ g of heavy chain/pBAN-2) was mixed in a sterile eppendorf, ethanol precipitated, and rinsed twice with 70% ethanol. DNA pellets were resuspended in sterile Tris-EDTA.
  • the DNA was diluted with 60 ⁇ l of 20 mM HEPES (pH 7.4) in a 5 ml polystyrene tube.
  • 120 ⁇ l of DOTAP liposomal transfection reagent (Boehringer Mannheim) was diluted with 80 ⁇ l of 20 mM HEPES (pH 7.4).
  • the DNA/HEPES was added to the diluted DOTAP, mixed gently, and left at room temperature for 15 min.
  • Culture medium IMDM + 5% FCS + HT
  • Microtitre plates were coated with 50 ⁇ l/well anti-human lg Fc (Sigma, catalogue number 1-2136) in PBS at 2.5 ⁇ g/mi overnight at 4°C. The coating Ab was removed and the plates were blocked by addition of 100 ⁇ l/well blocking buffer (PBS + 1 % BSA + 5% FCS + 1 % heat- inactivated normal rabbit serum (NRS) overnight at 4°C. The transfection supematants were added (50 ⁇ l/well) for at least 1h at room temperature. The wells were washed with PBS/0.5% Tween-20 (PBS/Tween).
  • PBS/Tween PBS/0.5% Tween-20
  • the wells were then washed with PBS/Tween and the transfection supematants were added (50 ⁇ l/well) for at least 1 h at room temperature.
  • the wells were washed withPBS/Tween and biotinylated sheep anti-human lg (Amersham, catalogue number RPN 1003) diluted 1/5000 in blocking buffer was added (50 ⁇ l/well) for 1h at room temperature.
  • the wells were washed with PBS/Tween and 50 ⁇ l/well ExtrAvidin-peroxidase (Sigma, catalogue number E-2886) was added for 30 min at room temperature.
  • Wild-type purified Campath-1H Ab binds strongly to recombinant Campath-1 Ag-fusion protein in ELISA assays.
  • the wild-type Ab can be titrated down in concentration until binding is just detectable. This may be referred to as "1 Ab binding unit”.
  • a suitable non-binding mutant of the wild-type will not show detectable binding at many times this concentration e.g. 100 times, or 1000 times, or preferably 10,000 times this concentration of wild -type Campath-1 H Ab.
  • transgenic mice are used.
  • transgenic mice expressing human CD52 behind a murine CD2 promoter to mimic the expression of CD52 on T-cells can be used.
  • mice a 2.8 kb genomic fragment containing the 2 exons of the human CD52 gene as well as 4.5 kb upstream and 3' flanking sequence of the human CD2 gene can be introduced into the genome of transgenic mice. It is thought that strong control regions are present 3' to the human CD2 gene that determine the high levels and tissue-specific expression of the gene (Greaves et al, 1989).
  • four CD52/CBA founders were established that transmitted the transgene. Indeed, when peripheral blood staining of their offspring was analysed by fluorescence activated cell sorting and 2-colour staining, it was shown that the cells expressing mouse CD3 (T-cells) also expressed human CD52 (D. Kioussis, unpublished data). These mice were bred to homozygosity and greater than 95% of their T-cells express high levels of human CD52 on the cell surface. These mice produce a vigorous anti-globulin response (titre of
  • an amount (e.g.500 mg) of the non-cell-binding form of the therapeutic antibody would be administered to a patient awaiting treatment with the therapeutic antibody.
  • the non-cell-binding antibody as administered is freshly deaggregated (for example by passage through a fine filter).
  • the monovalent form is a single-chain Fv [formed by the VL, a short peptide linker (such as those reviewed in Huston et al, 1991) and the mutated VH] genetically fused with the sequence encoding the hinge-CH2-CH3 of human lgG1.
  • This construct is expressed in association with a truncated heavy chain (hinge-CH2-CH3 only; Routledge et al, 1991) such that a protein is expressed that is composed essentially of a single Ab-combining site and a functional lg Fc domain.
  • SFv have been expressed in mammalian cells from a number of different antibodies and have been shown to fold into the correct conformation for antigen-binding by functional activity (Gilliland et al, 1996).
  • the Fc portion is a preferred feature which should ensure serum half-life comparable to the minimal mutant and to the wild-type therapeutic Ab, whilst monovalency will ensure that binding to CD52 is greatly reduced due to the decrease in avidity.
  • Section A1 We have already shown from the CDR- swapping experiments (section A1) that the Campath-1 Ab binds poorly to CD52 in a monovalent form.
  • Chothia C and Lesk A M 1987 Canonical structures for the hypervariable regions of immunoglobulins. J Mol. Biol. 196, 901-917. Chothia C, Lesk A M, Tramontano A, Levitt M, Smith-Gill S J, Air G, Sheriff S, Padlan E A, Davies D, Tulip W R, Colman P M, Spinelli S, Alzari P M, Poljak R J. 1989. Conformations of immunoglobulin hypervariable regions. Nature 342, 877-883.

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PCT/GB1997/000472 1996-02-20 1997-02-20 Antibody variants WO1997031024A1 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP97905226A EP0970127B1 (en) 1996-02-20 1997-02-20 Induction of immunological tolerance to a therapeutic antibody using variants of the said therapeutic antibody
NZ331299A NZ331299A (en) 1996-02-20 1997-02-20 Antibody variants with reduced affinity for a cell-surface antigen
JP52989397A JP4574750B2 (ja) 1996-02-20 1997-02-20 抗体変異物
AU18851/97A AU723366B2 (en) 1996-02-20 1997-02-20 Antibody variants
DE69738138T DE69738138T2 (de) 1996-02-20 1997-02-20 Induktion von immunologischer Toleranz gegen einen therapeutischen Antikörper durch Varianten des therapeutischen Antikörpers
CA002246715A CA2246715C (en) 1996-02-20 1997-02-20 Antibody variants
US11/180,631 US8440190B2 (en) 1996-02-20 2005-07-14 Antibody variants

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GB9603507.6 1996-02-20
GBGB9603507.6A GB9603507D0 (en) 1996-02-20 1996-02-20 Antibody variants

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US11/180,631 Division US8440190B2 (en) 1996-02-20 2005-07-14 Antibody variants

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EP (1) EP0970127B1 (enrdf_load_stackoverflow)
JP (1) JP4574750B2 (enrdf_load_stackoverflow)
AT (1) ATE373016T1 (enrdf_load_stackoverflow)
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CA (1) CA2246715C (enrdf_load_stackoverflow)
DE (1) DE69738138T2 (enrdf_load_stackoverflow)
ES (1) ES2294793T3 (enrdf_load_stackoverflow)
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US6037454A (en) * 1996-11-27 2000-03-14 Genentech, Inc. Humanized anti-CD11a antibodies
WO2002030460A3 (en) * 2000-10-09 2003-03-13 Isis Innovation Therapeutic antibodies
US6632926B1 (en) 1998-11-18 2003-10-14 Genentech, Inc. Antibody variants
US7465790B2 (en) 2000-10-09 2008-12-16 Isis Innovation, Inc. Therapeutic antibodies
US8399219B2 (en) 2009-02-23 2013-03-19 Cytomx Therapeutics, Inc. Protease activatable interferon alpha proprotein
US9120853B2 (en) 2012-04-27 2015-09-01 Cytomx Therapeutics, Inc. Activatable antibodies that bind epidermal growth factor receptor and methods of use thereof
US9169321B2 (en) 2007-08-22 2015-10-27 The Regents Of The University Of California Activatable binding polypeptides and methods of identification and use thereof
US9453078B2 (en) 2009-01-12 2016-09-27 Cytomx Therapeutics, Inc. Modified antibody compositions, methods of making and using thereof
US9540440B2 (en) 2013-10-30 2017-01-10 Cytomx Therapeutics, Inc. Activatable antibodies that bind epidermal growth factor receptor and methods of use thereof
US9737623B2 (en) 2013-12-11 2017-08-22 Cytomx Therapeutics, Inc. Antibodies that bind activatable antibodies and methods of use thereof
US11945874B2 (en) 2009-05-13 2024-04-02 Genzyme Corporation Anti-human CD52 immunoglobulins

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US20020048578A1 (en) 2002-04-25
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