WO1997011093A2 - Parainfluenza virus glycoproteins and vaccines - Google Patents
Parainfluenza virus glycoproteins and vaccines Download PDFInfo
- Publication number
- WO1997011093A2 WO1997011093A2 PCT/CA1996/000639 CA9600639W WO9711093A2 WO 1997011093 A2 WO1997011093 A2 WO 1997011093A2 CA 9600639 W CA9600639 W CA 9600639W WO 9711093 A2 WO9711093 A2 WO 9711093A2
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- WO
- WIPO (PCT)
- Prior art keywords
- piv
- glycoprotein
- glycoproteins
- parainfluenza virus
- purified
- Prior art date
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Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BR9610590-9A BR9610590A (en) | 1995-09-22 | 1996-09-23 | Parainfluenza virus glycoproteins and vaccines |
JP9512271A JPH11513372A (en) | 1995-09-22 | 1996-09-23 | Parainfluenza virus glycoproteins and vaccines |
US09/043,477 US6165774A (en) | 1995-09-22 | 1996-09-23 | Parainfluenza virus glycoproteins and vaccines |
AU69820/96A AU712213B2 (en) | 1995-09-22 | 1996-09-23 | Parainfluenza virus glycoproteins and vaccines |
EP96930931A EP0851873A2 (en) | 1995-09-22 | 1996-09-23 | Parainfluenza virus glycoproteins and vaccines |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US53246495A | 1995-09-22 | 1995-09-22 | |
US08/532,464 | 1995-09-22 |
Publications (2)
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WO1997011093A2 true WO1997011093A2 (en) | 1997-03-27 |
WO1997011093A3 WO1997011093A3 (en) | 1997-05-15 |
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Application Number | Title | Priority Date | Filing Date |
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PCT/CA1996/000639 WO1997011093A2 (en) | 1995-09-22 | 1996-09-23 | Parainfluenza virus glycoproteins and vaccines |
Country Status (9)
Country | Link |
---|---|
US (1) | US6165774A (en) |
EP (1) | EP0851873A2 (en) |
JP (1) | JPH11513372A (en) |
CN (1) | CN1285608C (en) |
AU (1) | AU712213B2 (en) |
BR (1) | BR9610590A (en) |
CA (1) | CA2232515A1 (en) |
MX (1) | MX9802285A (en) |
WO (1) | WO1997011093A2 (en) |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998053078A1 (en) * | 1997-05-23 | 1998-11-26 | The Government Of The United States Of America, As Represented By The Department Of Health And Humanservices | Production of attenuated parainfluenza virus vaccines from cloned nucleotide sequences |
US6410023B1 (en) | 1997-05-23 | 2002-06-25 | United States Of America | Recombinant parainfluenza virus vaccines attenuated by deletion or ablation of a non-essential gene |
US6656720B2 (en) | 1996-04-01 | 2003-12-02 | Chiron Behring Gmbh & Co. | Animal cells and processes for the replication of influenza viruses |
US6699476B1 (en) | 1996-07-15 | 2004-03-02 | Peter L. Collins | Production of recombinant respiratory syncytial viruses expressing immune modulatory molecules |
US7201907B1 (en) | 1997-05-23 | 2007-04-10 | The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Attenuated human-bovine chimeric parainfluenza virus(PIV) vaccines |
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US7250171B1 (en) | 1997-05-23 | 2007-07-31 | United States Of America As Represented By The Dept. Of Health & Human Services | Construction and use of recombinant parainfluenza viruses expressing a chimeric glycoprotein |
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US7485440B2 (en) | 1995-09-27 | 2009-02-03 | The United States Of America As Represented By The Department Of Health And Human Services | Production of attenuated respiratory syncytial virus vaccines involving modification of M2 ORF2 |
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US7709007B2 (en) | 1996-07-15 | 2010-05-04 | The United States Of America As Represented By The Department Of Health And Human Services | Production of attenuated respiratory syncytial virus vaccines from cloned nucleotide sequences |
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US7951383B2 (en) | 1997-05-23 | 2011-05-31 | The United States Of America As Represented By The Department Of Health And Human Services | Attenuated parainfluenza virus (PIV) vaccines |
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US10329536B2 (en) | 2001-09-12 | 2019-06-25 | Seqirus UK Limited | Methods for producing an active constituent of a pharmaceutical or a diagnostic agent in an MDCK cell suspension culture |
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Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2001326A (en) * | 1977-07-13 | 1979-01-31 | Sandoz Ltd | Glycoproteins from paramyxovirus and vaccines therefrom |
EP0222415A2 (en) * | 1985-11-15 | 1987-05-20 | Research Corporation | Viral glycoprotein subunit vaccine |
WO1988008718A1 (en) * | 1987-05-05 | 1988-11-17 | Molecular Engineering Associates, Inc. | Intranasal immunization against viral infection using viral glycoprotein subunit vaccine |
WO1991000104A1 (en) * | 1989-06-29 | 1991-01-10 | Connaught Laboratories Limited | Production of virus and purification of viral envelope proteins for vaccine use |
EP0522560A2 (en) * | 1991-07-11 | 1993-01-13 | IMMUNO Aktiengesellschaft | Method and composition for the prevention of Lyme disease |
US5256294A (en) * | 1990-09-17 | 1993-10-26 | Genentech, Inc. | Tangential flow filtration process and apparatus |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9120221D0 (en) * | 1991-09-23 | 1991-11-06 | Smithkline Beecham Biolog | Novel compounds |
-
1996
- 1996-09-23 BR BR9610590-9A patent/BR9610590A/en not_active Application Discontinuation
- 1996-09-23 CA CA002232515A patent/CA2232515A1/en not_active Abandoned
- 1996-09-23 EP EP96930931A patent/EP0851873A2/en not_active Withdrawn
- 1996-09-23 US US09/043,477 patent/US6165774A/en not_active Expired - Lifetime
- 1996-09-23 JP JP9512271A patent/JPH11513372A/en active Pending
- 1996-09-23 AU AU69820/96A patent/AU712213B2/en not_active Ceased
- 1996-09-23 WO PCT/CA1996/000639 patent/WO1997011093A2/en not_active Application Discontinuation
- 1996-09-23 CN CNB961984538A patent/CN1285608C/en not_active Expired - Fee Related
-
1998
- 1998-03-23 MX MX9802285A patent/MX9802285A/en unknown
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2001326A (en) * | 1977-07-13 | 1979-01-31 | Sandoz Ltd | Glycoproteins from paramyxovirus and vaccines therefrom |
EP0222415A2 (en) * | 1985-11-15 | 1987-05-20 | Research Corporation | Viral glycoprotein subunit vaccine |
WO1988008718A1 (en) * | 1987-05-05 | 1988-11-17 | Molecular Engineering Associates, Inc. | Intranasal immunization against viral infection using viral glycoprotein subunit vaccine |
WO1991000104A1 (en) * | 1989-06-29 | 1991-01-10 | Connaught Laboratories Limited | Production of virus and purification of viral envelope proteins for vaccine use |
US5256294A (en) * | 1990-09-17 | 1993-10-26 | Genentech, Inc. | Tangential flow filtration process and apparatus |
EP0522560A2 (en) * | 1991-07-11 | 1993-01-13 | IMMUNO Aktiengesellschaft | Method and composition for the prevention of Lyme disease |
Non-Patent Citations (21)
Cited By (17)
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US7485440B2 (en) | 1995-09-27 | 2009-02-03 | The United States Of America As Represented By The Department Of Health And Human Services | Production of attenuated respiratory syncytial virus vaccines involving modification of M2 ORF2 |
US6656720B2 (en) | 1996-04-01 | 2003-12-02 | Chiron Behring Gmbh & Co. | Animal cells and processes for the replication of influenza viruses |
US7709007B2 (en) | 1996-07-15 | 2010-05-04 | The United States Of America As Represented By The Department Of Health And Human Services | Production of attenuated respiratory syncytial virus vaccines from cloned nucleotide sequences |
US7465794B2 (en) | 1996-07-15 | 2008-12-16 | The United States Of America As Represented By The Department Of Health And Human Services | Polynucleotides encoding recombinant respiratory syncytial viruses expressing immune modulatory molecules |
US6699476B1 (en) | 1996-07-15 | 2004-03-02 | Peter L. Collins | Production of recombinant respiratory syncytial viruses expressing immune modulatory molecules |
US6410023B1 (en) | 1997-05-23 | 2002-06-25 | United States Of America | Recombinant parainfluenza virus vaccines attenuated by deletion or ablation of a non-essential gene |
US7250171B1 (en) | 1997-05-23 | 2007-07-31 | United States Of America As Represented By The Dept. Of Health & Human Services | Construction and use of recombinant parainfluenza viruses expressing a chimeric glycoprotein |
US7314631B1 (en) | 1997-05-23 | 2008-01-01 | The United States Of America As Represented By The Department Of Health And Human Services | Use of recombinant live-attenuated parainfluenza virus (PIV) as a vector to protect against disease caused by PIV and respiratory syncytial virus (RSV) |
US7208161B1 (en) | 1997-05-23 | 2007-04-24 | The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Production of attenuated parainfluenza virus vaccines from cloned nucleotide sequences |
US7201907B1 (en) | 1997-05-23 | 2007-04-10 | The United States Of America, Represented By The Secretary, Department Of Health And Human Services | Attenuated human-bovine chimeric parainfluenza virus(PIV) vaccines |
US7622123B2 (en) | 1997-05-23 | 2009-11-24 | The United States Of America As Represented By The Department Of Health And Human Services | Attenuated human-bovine chimeric parainfluenza virus (PIV) vaccines |
US7632508B2 (en) | 1997-05-23 | 2009-12-15 | The United States Of America | Attenuated human-bovine chimeric parainfluenza virus (PIV) vaccines |
WO1998053078A1 (en) * | 1997-05-23 | 1998-11-26 | The Government Of The United States Of America, As Represented By The Department Of Health And Humanservices | Production of attenuated parainfluenza virus vaccines from cloned nucleotide sequences |
US7951383B2 (en) | 1997-05-23 | 2011-05-31 | The United States Of America As Represented By The Department Of Health And Human Services | Attenuated parainfluenza virus (PIV) vaccines |
US7829102B2 (en) | 1999-07-09 | 2010-11-09 | The United States Of America As Represented By The Department Of Health And Human Services | Production of attenuated, human-bovine chimeric respiratory syncytial virus vaccines |
US10329536B2 (en) | 2001-09-12 | 2019-06-25 | Seqirus UK Limited | Methods for producing an active constituent of a pharmaceutical or a diagnostic agent in an MDCK cell suspension culture |
US10156576B2 (en) | 2010-12-16 | 2018-12-18 | National Institute Of Advanced Industrial Science And Technology | Method for enrichment and separation of spinal fluid glycoprotein, method for searching for marker for central nervous system diseases which utilizes the aforementioned method, and marker for central nervous system diseases |
Also Published As
Publication number | Publication date |
---|---|
MX9802285A (en) | 1998-11-29 |
CN1285608C (en) | 2006-11-22 |
JPH11513372A (en) | 1999-11-16 |
BR9610590A (en) | 2000-10-24 |
US6165774A (en) | 2000-12-26 |
CN1202905A (en) | 1998-12-23 |
EP0851873A2 (en) | 1998-07-08 |
CA2232515A1 (en) | 1997-03-27 |
WO1997011093A3 (en) | 1997-05-15 |
AU6982096A (en) | 1997-04-09 |
AU712213B2 (en) | 1999-11-04 |
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