WO1996001425A3 - Assay for inhibitors of dp-1 and other dp proteins - Google Patents

Assay for inhibitors of dp-1 and other dp proteins Download PDF

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Publication number
WO1996001425A3
WO1996001425A3 PCT/GB1995/001567 GB9501567W WO9601425A3 WO 1996001425 A3 WO1996001425 A3 WO 1996001425A3 GB 9501567 W GB9501567 W GB 9501567W WO 9601425 A3 WO9601425 A3 WO 9601425A3
Authority
WO
WIPO (PCT)
Prior art keywords
phosphorylation
proteins
inhibitors
assay
state
Prior art date
Application number
PCT/GB1995/001567
Other languages
French (fr)
Other versions
WO1996001425A2 (en
Inventor
Thangue Nicholas Barrie La
Original Assignee
Medical Res Council
Thangue Nicholas Barrie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to NZ288712A priority Critical patent/NZ288712A/en
Priority to AU28036/95A priority patent/AU698473B2/en
Priority to JP8503745A priority patent/JPH10502252A/en
Priority to US08/602,846 priority patent/US5871901A/en
Priority to EP95923486A priority patent/EP0769144B1/en
Priority to DE0769144T priority patent/DE769144T1/en
Application filed by Medical Res Council, Thangue Nicholas Barrie filed Critical Medical Res Council
Priority to DK95923486T priority patent/DK0769144T3/en
Priority to DE69513025T priority patent/DE69513025T2/en
Publication of WO1996001425A2 publication Critical patent/WO1996001425A2/en
Publication of WO1996001425A3 publication Critical patent/WO1996001425A3/en
Priority to NO19965584A priority patent/NO317397B1/en
Priority to FI965289A priority patent/FI965289A0/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The protein DP-1, part of the DP-1/E2F-1 transcription factor complex, as well as DP-2 and DP-3 has its phosphorylation level regulated during cell cycle progression. This finding allows assays to be based on changes in phosphorylation of DP proteins, in particular for agents which may affect the phosphorylation state of DP. DP-1 has been found to have a greater affinity to DNA when in a hypophosphorylated state. Also disclosed are antibodies that recognise phosphorylation sites on DP-1.
PCT/GB1995/001567 1994-07-01 1995-07-03 Assay for inhibitors of dp-1 and other dp proteins WO1996001425A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
AU28036/95A AU698473B2 (en) 1994-07-01 1995-07-03 Assay for inhibitors of DP-1 and other DP proteins
JP8503745A JPH10502252A (en) 1994-07-01 1995-07-03 Assay for inhibitors of DP-1 and other DP proteins
US08/602,846 US5871901A (en) 1994-07-01 1995-07-03 Assay for inhibitors of DP-1 and other DP proteins
EP95923486A EP0769144B1 (en) 1994-07-01 1995-07-03 Assay for inhibitors of dp-1 and other dp proteins
DE0769144T DE769144T1 (en) 1994-07-01 1995-07-03 TEST FOR INHIBITORS OF DP-1 AND OTHER DP PROTEINS
NZ288712A NZ288712A (en) 1994-07-01 1995-07-03 Assay for transcription factor dp-1 inhibitor
DK95923486T DK0769144T3 (en) 1994-07-01 1995-07-03 Assay for inhibitors of DP-1 and other DP proteins
DE69513025T DE69513025T2 (en) 1994-07-01 1995-07-03 TEST FOR INHIBITORS OF DP-1 AND OTHER DP PROTEINS
NO19965584A NO317397B1 (en) 1994-07-01 1996-12-27 Assay for inhibitors of DP-1 and DP proteins
FI965289A FI965289A0 (en) 1994-07-01 1996-12-31 Determination of inhibitors of DP-1 and other DP proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9413327A GB9413327D0 (en) 1994-07-01 1994-07-01 Assay for inhibitors of dp-1
GB9413327.9 1994-07-01

Publications (2)

Publication Number Publication Date
WO1996001425A2 WO1996001425A2 (en) 1996-01-18
WO1996001425A3 true WO1996001425A3 (en) 1996-03-14

Family

ID=10757696

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1995/001567 WO1996001425A2 (en) 1994-07-01 1995-07-03 Assay for inhibitors of dp-1 and other dp proteins

Country Status (13)

Country Link
US (1) US5871901A (en)
EP (1) EP0769144B1 (en)
JP (1) JPH10502252A (en)
AT (1) ATE186119T1 (en)
AU (1) AU698473B2 (en)
CA (1) CA2193091A1 (en)
DE (2) DE769144T1 (en)
DK (1) DK0769144T3 (en)
FI (1) FI965289A0 (en)
GB (1) GB9413327D0 (en)
NO (1) NO317397B1 (en)
NZ (1) NZ288712A (en)
WO (1) WO1996001425A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6127505A (en) * 1995-02-02 2000-10-03 Simula Inc. Impact resistant polyurethane and method of manufacture thereof
GB9610195D0 (en) * 1996-05-15 1996-07-24 Medical Res Council Assay
US6159691A (en) * 1996-05-15 2000-12-12 Prolifix Limited Assay for a putative regulator of cell cycle progression
EP0963999A4 (en) * 1996-09-30 2004-09-15 Kyowa Hakko Kogyo Kk E2f activity inhibitory compounds
US6387649B1 (en) 1996-09-30 2002-05-14 Prolifix Limited Assay for a regulator of cell cycle progression
WO1999053069A2 (en) * 1998-04-09 1999-10-21 E.I. Du Pont De Nemours And Company Cell cycle regulatory proteins cdc2 and pitslre from plants
GB0308711D0 (en) * 2003-04-15 2003-05-21 Univ The Glasgow Assay methods

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994010307A1 (en) * 1992-10-29 1994-05-11 Medical Research Council Transcription factor dp-1
WO1994018324A2 (en) * 1993-02-12 1994-08-18 The Salk Institute For Biological Studies Phospho-specific antibodies against transcription factors, more specifically against a creb derived peptide

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994010307A1 (en) * 1992-10-29 1994-05-11 Medical Research Council Transcription factor dp-1
WO1994018324A2 (en) * 1993-02-12 1994-08-18 The Salk Institute For Biological Studies Phospho-specific antibodies against transcription factors, more specifically against a creb derived peptide

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
K. JOOS ET AL.: "Proto-oncogenic properties of the DP family of proteins.", ONCOGENE, vol. 10, no. 8, 20 April 1995 (1995-04-20), pages 1529 - 1536 *
L. R. BANDARA ET AL.: "DP-1: a cell cycle-regulated and phosphorylated component of transcription factor DRTF1/E2F which is functionally important for recognition by pRb and the adenovirus E4 orf 6/7 protein.", THE EMBO JOURNAL, vol. 13, no. 13, 1 July 1994 (1994-07-01), pages 3104 - 3114 *

Also Published As

Publication number Publication date
DE69513025D1 (en) 1999-12-02
NO965584L (en) 1997-02-28
AU698473B2 (en) 1998-10-29
DE69513025T2 (en) 2000-04-13
DK0769144T3 (en) 2000-05-08
DE769144T1 (en) 1997-07-10
JPH10502252A (en) 1998-03-03
NZ288712A (en) 1998-09-24
NO965584D0 (en) 1996-12-27
US5871901A (en) 1999-02-16
CA2193091A1 (en) 1996-01-18
WO1996001425A2 (en) 1996-01-18
EP0769144A2 (en) 1997-04-23
NO317397B1 (en) 2004-10-25
ATE186119T1 (en) 1999-11-15
FI965289A (en) 1996-12-31
FI965289A0 (en) 1996-12-31
AU2803695A (en) 1996-01-25
EP0769144B1 (en) 1999-10-27
GB9413327D0 (en) 1994-08-24

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