WO1995015391A3 - Aspergillus expression system - Google Patents

Aspergillus expression system Download PDF

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Publication number
WO1995015391A3
WO1995015391A3 PCT/US1994/013613 US9413613W WO9515391A3 WO 1995015391 A3 WO1995015391 A3 WO 1995015391A3 US 9413613 W US9413613 W US 9413613W WO 9515391 A3 WO9515391 A3 WO 9515391A3
Authority
WO
WIPO (PCT)
Prior art keywords
expression system
aspergillus expression
aspergillus
expression
japonicus
Prior art date
Application number
PCT/US1994/013613
Other languages
French (fr)
Other versions
WO1995015391A2 (en
Inventor
Randy M Berka
Wendy Yoder
Shinobu Takagi
Karuppan Chettier Boominathan
Original Assignee
Novo Nordisk Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk Biotech Inc filed Critical Novo Nordisk Biotech Inc
Priority to AU11019/95A priority Critical patent/AU695766B2/en
Priority to EP95901976A priority patent/EP0730655A1/en
Priority to JP07515688A priority patent/JP3117997B2/en
Publication of WO1995015391A2 publication Critical patent/WO1995015391A2/en
Publication of WO1995015391A3 publication Critical patent/WO1995015391A3/en
Priority to FI962288A priority patent/FI962288A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C12N9/242—Fungal source

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a novel expression system in which A. japonicus-type species are used as host cells for expression of heterologous proteins.
PCT/US1994/013613 1993-12-01 1994-11-29 Aspergillus expression system WO1995015391A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU11019/95A AU695766B2 (en) 1993-12-01 1994-11-29 (Aspergillus) expression system
EP95901976A EP0730655A1 (en) 1993-12-01 1994-11-29 $i(ASPERGILLUS) EXPRESSION SYSTEM
JP07515688A JP3117997B2 (en) 1993-12-01 1994-11-29 Aspergillus expression system
FI962288A FI962288A (en) 1993-12-01 1996-05-31 Aspergillus expression system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16167593A 1993-12-01 1993-12-01
US08/161,675 1993-12-01

Publications (2)

Publication Number Publication Date
WO1995015391A2 WO1995015391A2 (en) 1995-06-08
WO1995015391A3 true WO1995015391A3 (en) 1995-08-03

Family

ID=22582238

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1994/013613 WO1995015391A2 (en) 1993-12-01 1994-11-29 Aspergillus expression system

Country Status (8)

Country Link
US (1) US5667990A (en)
EP (1) EP0730655A1 (en)
JP (2) JP3117997B2 (en)
CN (1) CN1139457A (en)
AU (1) AU695766B2 (en)
CA (1) CA2178007A1 (en)
FI (1) FI962288A (en)
WO (1) WO1995015391A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5646025A (en) * 1995-05-05 1997-07-08 Novo Nordisk A/S Scytalidium catalase gene
AU6121796A (en) * 1995-06-16 1997-01-15 Novo Nordisk A/S A process for producing trypsin (trypsinogen)
US5945328A (en) * 1995-06-16 1999-08-31 Novo Nordisk A/S Process for producing trypsin (trypsinogen)
JP3958884B2 (en) 1998-09-11 2007-08-15 株式会社林原生物化学研究所 Non-reducing saccharide-forming enzyme, trehalose-releasing enzyme, and method for producing saccharide using the enzyme
WO2000039322A1 (en) * 1998-12-23 2000-07-06 Novozymes A/S Methods for producing polypeptides in aspergillus mutant cells
US6383781B1 (en) 1998-12-23 2002-05-07 Novozymes A/S Methods for producing polypeptides in aspergillus mutant cells
WO2001025406A1 (en) * 1999-10-01 2001-04-12 University Of Victoria Innovation & Development Corporation Mannosidases and methods for using same
CN1256029C (en) * 2001-01-10 2006-05-17 Dsmip资产有限公司 Use of food and drink as delevery system for phytase in humans
DK2119781T3 (en) * 2007-01-30 2012-11-26 Mitsubishi Kagaku Foods Corp glyceroglycolipid lipase
US9493744B2 (en) * 2012-06-20 2016-11-15 Genentech, Inc. Methods for viral inactivation and other adventitious agents
WO2014073674A1 (en) * 2012-11-09 2014-05-15 国立大学法人東北大学 Method for manufacturing useful substance in which high-density cultured strain of filamentous fungi is used
CN103058971B (en) * 2013-01-17 2014-08-06 福州大学 Benzoquinone spiro compound derived from aspergillus aculeatus and application of benzoquinone spiro compound
CN107988085B (en) * 2017-12-14 2019-11-08 青岛蔚蓝生物集团有限公司 A kind of microorganism Aspergillus aculeatus bacterial strain of high yield acid pectase and its application
CN107988086B (en) * 2017-12-14 2019-11-08 青岛蔚蓝生物集团有限公司 A kind of bacterial strain of high yield rhamnosan galacturonic hydrolase

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0305216A1 (en) * 1987-08-28 1989-03-01 Novo Nordisk A/S Recombinant Humicola lipase and process for the production of recombinant humicola lipases
EP0565172A1 (en) * 1992-04-10 1993-10-13 Quest International B.V. Cloning and expression of DNA encoding a ripening form of a polypeptide having sulfhydryl oxidase activity

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0305216A1 (en) * 1987-08-28 1989-03-01 Novo Nordisk A/S Recombinant Humicola lipase and process for the production of recombinant humicola lipases
EP0565172A1 (en) * 1992-04-10 1993-10-13 Quest International B.V. Cloning and expression of DNA encoding a ripening form of a polypeptide having sulfhydryl oxidase activity

Also Published As

Publication number Publication date
CA2178007A1 (en) 1995-06-08
WO1995015391A2 (en) 1995-06-08
FI962288A0 (en) 1996-05-31
JPH09500543A (en) 1997-01-21
AU1101995A (en) 1995-06-19
AU695766B2 (en) 1998-08-20
US5667990A (en) 1997-09-16
FI962288A (en) 1996-07-26
JP2001025393A (en) 2001-01-30
JP3117997B2 (en) 2000-12-18
CN1139457A (en) 1997-01-01
EP0730655A1 (en) 1996-09-11

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