WO1994028110A1 - Detection apparatus - Google Patents

Detection apparatus Download PDF

Info

Publication number
WO1994028110A1
WO1994028110A1 PCT/GB1994/001162 GB9401162W WO9428110A1 WO 1994028110 A1 WO1994028110 A1 WO 1994028110A1 GB 9401162 W GB9401162 W GB 9401162W WO 9428110 A1 WO9428110 A1 WO 9428110A1
Authority
WO
WIPO (PCT)
Prior art keywords
filter material
chambers
housing
chamber
additionally comprises
Prior art date
Application number
PCT/GB1994/001162
Other languages
French (fr)
Inventor
Peter Leonard Grant
Original Assignee
Celsis International Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celsis International Plc filed Critical Celsis International Plc
Priority to DE69404659T priority Critical patent/DE69404659T2/en
Priority to US08/557,112 priority patent/US5811257A/en
Priority to JP7500407A priority patent/JPH09505722A/en
Priority to AU68023/94A priority patent/AU668652B2/en
Priority to EP94916322A priority patent/EP0703976B1/en
Publication of WO1994028110A1 publication Critical patent/WO1994028110A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Definitions

  • This invention relates to apparatus suitable for use in testing liquid samples for the presence of microorganisms.
  • Apparatus for the detection of microorganisms in liquid samples is known, the use of which involves the introduction of the sample, optionally followed by wash liquid, and then growth medium, to encourage the organism to multiply.
  • the sample may be an antibiotic which, if residual material remains, can inhibit the growth of organisms thereby giving false negative results.
  • the difficulty encountered with such apparatus is that residual sample may become trapped in corners of the apparatus and is difficult to remove.
  • the present invention provides simple apparatus, suitable for manual use, which can obviate the problem described above and provide for both biological detection and optimised sterility testing.
  • the novel assay device comprises a housing having two chambers therewithin, both chambers being partially defined by a filter material and each having a liquid inlet, the device comprising also means for holding the filter material in a first or second position, respectively, such that the chambers are either separate or in communication.
  • the region of contact between the filter material and the chamber division, where residual sample may collect while the said means is maintained in the first position can be simply and effectively washed by selecting the second position.
  • a further advantage of the novel apparatus is that is it well adapted to the use of sensitive bioluminescence assays, and by contrast with conventional turbidity observation.
  • the drawing shows a generally cylindrical housing 10 having an internal cylindrical wall 11 that effectively divides the housing into respective cylindrical and annular chambers 12, 13. These chambers have respective liquid inlets 14, 15 (although one inlet may be replaced by an internal communication) , and are partially defined by a common filter material 16.
  • the drawing shows also a complementary generally cylindrical part 20 that is engageable with the housing 10, suitably by means of complementary circumferential engagement means 21, e.g., a snap-fit or threaded engagement (if the latter, the housing 10 and part 20 may be held together at all times, in engagement to a greater or lesser degree defining the first and second positions of the filter material 16, as discussed below).
  • the part 20 comprises an internal generally cylindrical wall 22 corresponding to wall 11, and including perforations 23 such that the respective generally cylindrical and annular volumes 24, 25 are in communication.
  • the part 20 also includes a liquid outlet 26.
  • the housing 10 and part 20 are firmly engaged, such that their respective walls 11 and 22 are substantially in contact, and may compress the filter material 16 therebetween.
  • the chambers 12 and 13 are separate.
  • Sample is now introduced through inlet 15 into the chamber 12, and liquid is allowed or caused to drain through the filter material 16 and then out of the device through the outlet 26.
  • Sample may have collected at the interface of the wall 11 and the filter material 16, and possibly beyond the wall 11.
  • the filter material can now be effectively washed by moving or partially disengaging the part 20, so that the filter material 16 is no longer held in close contact with the wall 11, i.e., in the "second position" and introducing a wash material through inlet 14.
  • a growth medium may now be introduced through the inlet 15 into the chamber 12, to allow the growth of microorganisms retained on the filter material 16 unless that is inhibited by antibiotic. Following growth, liquid can be removed through the outlet 26.
  • the inlets 14 and 15 are closed, the housing 10 inverted, and the growth medium is washed into contact with a second filter material 17 that partially defines a member 18 that can be separated from the housing 10, usually by means of a frangible, male-female or other connection at 19.
  • This separable member 18 is suitably in the form of a cuvette that can be introduced directly into a suitable analytical instrument, or retained by the user for future analysis.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Processing Of Meat And Fish (AREA)
  • Control And Other Processes For Unpacking Of Materials (AREA)
  • Push-Button Switches (AREA)
  • Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)

Abstract

An assay device comprises a housing (10) having two chambers (12, 13) therewithin, each chamber being partially defined by a first filter material (16) and each having a liquid inlet (14, 15), the device comprising also means (20) for holding the filter material in a first or second position, respectively, such that the chambers are either separate or in communication. This arrangement allows assays to be made more accurately, since material that is otherwise retained in the corners of the assay chamber can simply be washed away.

Description

DETECTION APPARATUS Field of the Invention
This invention relates to apparatus suitable for use in testing liquid samples for the presence of microorganisms.
Background of the Invention
Apparatus for the detection of microorganisms in liquid samples is known, the use of which involves the introduction of the sample, optionally followed by wash liquid, and then growth medium, to encourage the organism to multiply. In some cases, the sample may be an antibiotic which, if residual material remains, can inhibit the growth of organisms thereby giving false negative results. The difficulty encountered with such apparatus is that residual sample may become trapped in corners of the apparatus and is difficult to remove. Summary of the Invention
The present invention provides simple apparatus, suitable for manual use, which can obviate the problem described above and provide for both biological detection and optimised sterility testing. The novel assay device comprises a housing having two chambers therewithin, both chambers being partially defined by a filter material and each having a liquid inlet, the device comprising also means for holding the filter material in a first or second position, respectively, such that the chambers are either separate or in communication.
In use of the apparatus, the region of contact between the filter material and the chamber division, where residual sample may collect while the said means is maintained in the first position, can be simply and effectively washed by selecting the second position. A further advantage of the novel apparatus is that is it well adapted to the use of sensitive bioluminescence assays, and by contrast with conventional turbidity observation. Description of the Invention
The invention will now be described by way of example only with reference to the accompanying drawing, which is a schematic sectional view of a device embodying the present invention.
The drawing shows a generally cylindrical housing 10 having an internal cylindrical wall 11 that effectively divides the housing into respective cylindrical and annular chambers 12, 13. These chambers have respective liquid inlets 14, 15 (although one inlet may be replaced by an internal communication) , and are partially defined by a common filter material 16.
The drawing shows also a complementary generally cylindrical part 20 that is engageable with the housing 10, suitably by means of complementary circumferential engagement means 21, e.g., a snap-fit or threaded engagement (if the latter, the housing 10 and part 20 may be held together at all times, in engagement to a greater or lesser degree defining the first and second positions of the filter material 16, as discussed below). The part 20 comprises an internal generally cylindrical wall 22 corresponding to wall 11, and including perforations 23 such that the respective generally cylindrical and annular volumes 24, 25 are in communication. The part 20 also includes a liquid outlet 26.
In use, the housing 10 and part 20 are firmly engaged, such that their respective walls 11 and 22 are substantially in contact, and may compress the filter material 16 therebetween. In this "first" position, the chambers 12 and 13 are separate.
Sample is now introduced through inlet 15 into the chamber 12, and liquid is allowed or caused to drain through the filter material 16 and then out of the device through the outlet 26. Sample may have collected at the interface of the wall 11 and the filter material 16, and possibly beyond the wall 11. However, the filter material can now be effectively washed by moving or partially disengaging the part 20, so that the filter material 16 is no longer held in close contact with the wall 11, i.e., in the "second position" and introducing a wash material through inlet 14. A growth medium may now be introduced through the inlet 15 into the chamber 12, to allow the growth of microorganisms retained on the filter material 16 unless that is inhibited by antibiotic. Following growth, liquid can be removed through the outlet 26. According to a particularly preferred embodiment of the invention, the inlets 14 and 15 are closed, the housing 10 inverted, and the growth medium is washed into contact with a second filter material 17 that partially defines a member 18 that can be separated from the housing 10, usually by means of a frangible, male-female or other connection at 19. This separable member 18 is suitably in the form of a cuvette that can be introduced directly into a suitable analytical instrument, or retained by the user for future analysis. By the provision of several members 18 that can be releasably connected to the housing 10, the novel device can be used for multiple assays.

Claims

1. An assay device comprising a housing (10) having two chambers (12,13) therewithin, each chamber being partially defined by a first filter material (16) and each having a liquid inlet (14,15) , the device comprising also means (20) for holding the filter material in a first or second position, respectively, such that the chambers are either separate or in communication.
2. A device according to claim 1, which additionally comprises a part (20) engageable with the housing and providing the said means according to the degree of engagement.
3. A device according to claim 1 or claim 2, in which one chamber (12) is surrounded by the other (13), e.g., the latter is annular.
4. A device according to any preceding claim, which additionally comprises a separable member (18) partially defined by a second filter material (17) and in communication with one of the chambers (12) .
5. A method for retaining an analyte in a liquid sample, which comprises: introducing the sample into one of the chambers (12) of a device according to any preceding claim and through the first filter material in the first position; and introducing a wash liquid into the other chamber (13) , when the first filter material is in the second position.
6. A method according to claim 5, in which the device is as defined in claim 4, which additionally comprises inverting the device and separating the separable member, when desired.
7. A method according to claim 5 or claim 6, wherein the analyte is a microorganism.
8. A method according to claim 7, which additionally comprises introducing a growth medium.
PCT/GB1994/001162 1993-06-01 1994-05-27 Detection apparatus WO1994028110A1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DE69404659T DE69404659T2 (en) 1993-06-01 1994-05-27 DEVICE FOR DETECTION
US08/557,112 US5811257A (en) 1993-06-01 1994-05-27 Detection apparatus
JP7500407A JPH09505722A (en) 1993-06-01 1994-05-27 Detector
AU68023/94A AU668652B2 (en) 1993-06-01 1994-05-27 Detection apparatus
EP94916322A EP0703976B1 (en) 1993-06-01 1994-05-27 Detection apparatus

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB9311242.3 1993-06-01
GB939311242A GB9311242D0 (en) 1993-06-01 1993-06-01 Detection apparatus

Publications (1)

Publication Number Publication Date
WO1994028110A1 true WO1994028110A1 (en) 1994-12-08

Family

ID=10736419

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1994/001162 WO1994028110A1 (en) 1993-06-01 1994-05-27 Detection apparatus

Country Status (8)

Country Link
US (1) US5811257A (en)
EP (1) EP0703976B1 (en)
JP (1) JPH09505722A (en)
AU (1) AU668652B2 (en)
DE (1) DE69404659T2 (en)
ES (1) ES2106543T3 (en)
GB (1) GB9311242D0 (en)
WO (1) WO1994028110A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998004675A2 (en) * 1996-07-29 1998-02-05 Pall Corporation Method for quantitation of microorganism contamination of liquids and apparatus therefor

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6428975B1 (en) * 2000-01-14 2002-08-06 Blue Ridge Pharmaceuticals, Inc. Methods for determining the presence or absence of microorganisms in lipid-containing compositions
DE102009052671B4 (en) * 2009-11-12 2015-07-30 Sartorius Stedim Biotech Gmbh Apparatus and method for treating a filtration medium

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990013624A1 (en) * 1989-05-08 1990-11-15 Human Medical Laboratories, Inc. Microorganism growth culture system, method and filtration unit utilized therewith
WO1991018653A2 (en) * 1990-06-04 1991-12-12 Molecular Devices Corporation Cell assay device
WO1992017261A1 (en) * 1991-04-04 1992-10-15 Nicholson Precision Instruments Inc. A vacuum clamped multi-sample filtration apparatus and method

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4829005A (en) * 1984-06-15 1989-05-09 Friedman Michael P Sedimentation filtration microorganism growth culture system
US5358690A (en) * 1989-01-10 1994-10-25 Lamina, Ltd. Environmental sample collection and membrane testing device

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990013624A1 (en) * 1989-05-08 1990-11-15 Human Medical Laboratories, Inc. Microorganism growth culture system, method and filtration unit utilized therewith
WO1991018653A2 (en) * 1990-06-04 1991-12-12 Molecular Devices Corporation Cell assay device
WO1992017261A1 (en) * 1991-04-04 1992-10-15 Nicholson Precision Instruments Inc. A vacuum clamped multi-sample filtration apparatus and method

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998004675A2 (en) * 1996-07-29 1998-02-05 Pall Corporation Method for quantitation of microorganism contamination of liquids and apparatus therefor
WO1998004675A3 (en) * 1996-07-29 1998-03-05 Pall Corp Method for quantitation of microorganism contamination of liquids and apparatus therefor
US5820767A (en) * 1996-07-29 1998-10-13 Pall Corporation Method for quantitation of microorganism contamination of liquids
GB2329394A (en) * 1996-07-29 1999-03-24 Pall Corp Method for quantitation of microorganism contamination of liquids and apparatus therefor
US5979668A (en) * 1996-07-29 1999-11-09 Pall Corporation Filtration device for quantitation of microorganism contaminated liquids
GB2329394B (en) * 1996-07-29 2000-11-22 Pall Corp Method for quantitation of microorganism contamination of liquids and apparatus therefor

Also Published As

Publication number Publication date
ES2106543T3 (en) 1997-11-01
GB9311242D0 (en) 1993-07-21
DE69404659T2 (en) 1997-11-27
US5811257A (en) 1998-09-22
DE69404659D1 (en) 1997-09-04
EP0703976B1 (en) 1997-07-30
EP0703976A1 (en) 1996-04-03
JPH09505722A (en) 1997-06-10
AU6802394A (en) 1994-12-20
AU668652B2 (en) 1996-05-09

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