WO1994022448A1 - Topical and systemic application of buspirone or derivatives thereof for treatment of pathological conditions associated with immune responses - Google Patents

Topical and systemic application of buspirone or derivatives thereof for treatment of pathological conditions associated with immune responses Download PDF

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Publication number
WO1994022448A1
WO1994022448A1 PCT/US1994/003353 US9403353W WO9422448A1 WO 1994022448 A1 WO1994022448 A1 WO 1994022448A1 US 9403353 W US9403353 W US 9403353W WO 9422448 A1 WO9422448 A1 WO 9422448A1
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WO
WIPO (PCT)
Prior art keywords
alkyl
aryl
buspirone
group
heterocycle
Prior art date
Application number
PCT/US1994/003353
Other languages
French (fr)
Inventor
Richard J. Sharpe
Kenneth A. Arndt
Stephen J. Galli
Peter C. Meltzer
Raj K. Razdan
Howard P. Sard
Original Assignee
Beth Israel Hospital Association
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/037,271 external-priority patent/US5484788A/en
Priority claimed from US08/037,225 external-priority patent/US5631017A/en
Application filed by Beth Israel Hospital Association filed Critical Beth Israel Hospital Association
Priority to AT94911001T priority Critical patent/ATE241359T1/en
Priority to DE69432749T priority patent/DE69432749T2/en
Priority to CA002159091A priority patent/CA2159091C/en
Priority to EP94911001A priority patent/EP0690715B1/en
Priority to JP6522262A priority patent/JPH09502699A/en
Priority to DK94911001T priority patent/DK0690715T3/en
Priority to AU63692/94A priority patent/AU692764B2/en
Publication of WO1994022448A1 publication Critical patent/WO1994022448A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/445—Non condensed piperidines, e.g. piperocaine
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
    • A61K31/505—Pyrimidines; Hydrogenated pyrimidines, e.g. trimethoprim
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders

Definitions

  • the immune system specifically recognizes and selectively eliminates foreign invaders, or other antigenic agents, by a process known as the immune response.
  • the immune response has three major characteristics: it responds adaptively to foreign invaders, it exhibits strong specificity, and it displays a long-term memory of earlier contacts with specific foreign pathogens or antigens.
  • the immune response involves the production of
  • T lymphocytes are highly specific for the antigen or hapten.
  • the immune response When directed against an infectious organism, the immune response can provide great benefit to the host.
  • an important component of current public health practices is the use of vaccines to elicit immune responses against infectious organisms that cause severe illness and death.
  • agents that are relatively innocuous such as pollen, animal dander, and certain plant resins
  • the cells, antibodies, and mediators which represent the effector components of the immune response can cause damage to the host's tissues that is out of proportion to any threat to health posed by the antigenic agent that first elicited the response.
  • Topical disorders that involve the immune system can result in result in one or more of the
  • Cutaneous contact hypersensitivity responses are complex expressions of cellular immunity
  • T cells are required for the expression and immunological specificity of the response, many other cell types also have roles in the reaction, including
  • Langerhans' cells Langerhans' cells, keratinocytes, and vascular endothelial cells. Antigen presentation is thought to be effected primarily by Langerhans' cells, whereas much of the local expression of the response is thought to be regulated by cytokines derived from both T cells and accessory cells.
  • serotonin (5-hydroxytryptamine, 5-HT)
  • serotonin has been shown to have a wide range of actions on T cells and other effector cells in vitro or in vivo , and pharmacological agents that deplete or
  • antagonize serotonin can diminish expression of cell-mediated immunity.
  • Early studies raised the possibility that such agents might reduce cell- mediated immunity by antagonizing or depleting mast cell-associated serotonin.
  • more recent findings indicate that at least one of these drugs, reserpine, can inhibit contact hypersensitivity independently of mast cells, probably through direct effects on T cells.
  • cutaneous contact hypersensitivity responses can occur on exposure to certain plant resins, such as those of poison ivy, and other commonly encountered agents in the environment. In individuals sensitized to such commonly encountered agents, a severe contact reaction can result upon exposure, with significant associated morbidity. Severe or repeated contact hypersensitivity reactions can be followed by significant chronic changes, such as scarring of affected tissues, itchiness, swelling, scaling and oozing of tissue fluid through the skin surface. This pathology may predispose the patient to bacterial superinfection. In the eye, chronic immune responses can lead to diminished vision or actual blindness. In the lung, chronic immune responses, such as chronic allergic asthma, can result in serious chronic lung disease.
  • hypersensitivity including systemic disorders, include but are not limited to host rejection of foreign organ or tissue transplants; graft-vs-host disease in which donor immunological cells present in the graft attack host tissues in the recipient of the graft; diseases with proven or possible autoimmune components, such as rheumatoid arthritis and juvenile rheumatoid arthritis, aphthous ulcer, lichen planus, psoriatic arthritis, psoriasis, excema, conjunctivitis, Sjogren's Syndrome, including keratoconjunctivitis sicca secondary to Sjogren's Syndrome, ulceris, alopecia areata, cutaneous lupus erythematosus, scleroderma, vaginitis, proctitis, drug eruptions, leprosy reversal reactions, erythema nodosum leprosum, autoimmune uveitis, multiple sclerosis, allergic encephalomyelitis, systemic lup
  • immunosuppressants including steroid hormones, antiproliferatives such as methotrexate and azathioprine, cyclosporine, alkylating agents such as cyclophosamide and busulfan, psoralen plus ultraviolet A (PUVA), and antibiotics.
  • steroid hormones such as methotrexate and azathioprine
  • cyclosporine such as cyclosporine
  • alkylating agents such as cyclophosamide and busulfan
  • PUVA psoralen plus ultraviolet A
  • Corticosteroids when administered systemically, can be effective but can be associated with
  • Topically applied corticosteroids have some efficacy in treating these conditions, but are only partially effective in many instances and have their own significant side effects, including atrophy of tissue, formation of telangiectasia, blanching, and a myriad of systemic effects if significantly absorbed. As a result, there still remains a strong need to provide new
  • immunosuppressive agents that can minimize or prevent pathological immune responses.
  • inflammatory response is a pathologic condition that can occur in response to immunologically non-specific injury, either from physical (such as trauma), chemical, or biological agents.
  • An inflammatory response is characterized by increased blood flow and redness in the inflamed area, increased capillary permeability and edema, and recruitment of immunologically non-specific white blood cells, especially neutrophils, that remove injurious material and promote repair.
  • inflammatory responses do not respond adaptively to the inciting stimulus, do not show specificity and do not exhibit long term memory.
  • Cellular products of lymphocytes may contribute to or induce an inflammatory response.
  • a compound can function as an anti-inflammatory agent without having immunosuppressive properties.
  • Phenylbutazone, indomethacin, aspirin, ibuprofen, and acetaminophen are examples of anti-inflammatory compounds which have no significant
  • immunologically mediated responses such as contact hypersensitivity.
  • PCT International Publication No. WO 91/02527 discloses a method and composition to treat cutaneous, mucosal, or ocular hypersensitivity that includes administering an effective amount of reserpine, spiperone, or other serotonin
  • invention to present a method for the systemic treatment of pathogenic conditions associated with immune responses.
  • a method for the treatment of a human or other mammal in need of immunosuppression is disclosed in which the mammal is treated topically or
  • buspirone or a buspirone derivative in a pharmaceutically- acceptable diluent or carrier for topical or systemic administration.
  • Buspirone and its active derivatives can be administered as general immunosuppressive agents to treat a variety of disorders.
  • a method is provided for the treatment of a
  • buspirone or a buspirone derivative or its pharmaceutically acceptable salt in a pharmaceutically-acceptable diluent or carrier for topical application.
  • the compounds are useful as topical agents in treating contact dermatitis, atopic dermatitis, eczematous dermatitis, psoriasis, Sjogren's Syndrome,
  • keratoconjunctivitis sicca secondary to Sjogren's Syndrome including keratoconjunctivitis sicca secondary to Sjogren's Syndrome, alopecia areata, allergic responses due to arthropod bite reactions, Crohn's disease, aphthous ulcer, ulceris, conjunctivitis, keratoconjunctivitis, ulcerative colitis, asthma, allergic asthma, cutaneous lupus erythematosus, scleroderma, vaginitis, proctitis, and drug eruptions.
  • the compounds may also be useful in reducing the infiltration of skin by malignant leukocytes in diseases such as mycosis fungoides. These compounds are also effective in treating an aqueous-deficient dry eye state (such as immune mediated keratoconjunctivitis) in a patient suffering therefrom, by administering the compound topically to the eye.
  • buspirone or its derivative is administered systemically for the treatment of systemic or topical disorders involving the immune system.
  • the parent buspirone can have a neuroleptic effect when administered systemically (but not typically when administered topically), however, it is a model of an active immunosuppressant.
  • Derivatives of buspirone are considered to be immunosuppressants if they suppress the ear swelling associated with an experimental contact hypersensitivity response by at least 40% at 24 hours after specific antigen challenge.
  • Figure 1 Effect of topically administered buspirone HCl on tissue swelling associated with oxazolone-induced contact hypersensitivity
  • Oxazolone (10 ⁇ l of a 0.5% (w:w) solution) was applied to both ears of all mice and the change in ear thickness was measured at a specified interval thereafter.
  • Buspirone HCl 100 mg/ml (Group B) or vehicle alone (Group A) was applied to the right ear of Balb/c mice 2 hours after challenge.
  • the change in ear thickness (post-challenge value minus baseline pre-challenge value) was measured 24 hours after oxazolone challenge.
  • the data are presented as the mean ⁇ SEM (standard error of the mean).
  • Figure 3 Comparative effects of 50 mg/kg subcutaneous administration of mianserin HCl (Group A) , trazadone HCl (Group B), haloperidol (Group C), buspirone HCl (Group D), and vehicle (Group E) on the tissue swelling associated with oxazolone- induced cutaneous contact hypersensitivity
  • Buspirone HCl, the other agents, or vehicle alone were administered to BALB/c mice 1 hour after right ears only were challenge for contact hypersensitivity.
  • the change in ear thickness (post-challenged value minus baseline pre-challenge value) was measured 24 hours after oxazolone challenge.
  • the data are presented as the mean ⁇ SEM.
  • Figure 4 Comparative effects of subcutaneous administration of 50 mg/kg mianserin HCl (Group A), trazadone HCl (Group B), haloperidol (Group C), buspirone HCl (Group D), and systemic vehicle
  • Oxazolone was applied to both ears of all mice at different times either pre- or post- buspirone HCl treatment and the change in ear thickness was measured at a specified interval thereafter. a. Two hours before oxazolone
  • buspirone HCl in Vehicle-N was applied to both surfaces of the right ears of Group B mice, whereas vehicle alone (0% buspirone HCl) was applied to both surfaces of the ears of control vehicle only
  • mice (Group A vehicle-N) mice.
  • the change in ear thickness was determined 24 hours after treatment with buspirone HCl, i.e. at 48 hours after
  • Buspirone HCl (Groups A and B) or vehicle alone (Group C) was administered to Balb/c mice 1 hour after challenge. Change in ear thickness (postchallenge - baseline pre-challenge value) was measured 24 hours after oxazolone challenge. The data are presented as ⁇ SEM. Systemic treatment with buspirone HCl (at 500 or 50 mg/kg)
  • FIG. 9 The effect of topical treatment with buspirone HCl on suppression of the sensitization phase of oxazolone challenge.
  • Buspirone HCl at 100 mg/ml (Group A) or vehicle-N (Group B) was applied to abdomen of mice 3 days prior to sensitization of Balb/c mice with 4% oxazolone. This treatment was repeated 3 days after sensitization. The right ears of all mice were then challenged with 0.5% oxazolone. The change in the ear thickness was measured 24 hours after oxazolone challenge. The data are presented as the mean ⁇ SEM. The
  • FIG. 10 Effect of topical treatment with buspirone HCl (100 mg/ml) on leukocyte infiltration associated with suppression of sensitization phase of oxazolone challenge. These data are from the same mice whose ear thickness measurements are presented in Fig. 9. Biopsies were performed 24 hours after oxazolone challenge.
  • FIG 11 Effect of systemic buspirone HCl treatment (50 mg/kg, administered subcutaneously), indomethacin, or placebo pellets (0.05 mg/pellet, implanted subcutaneously) on oxazolone induced contact hypersensitivity.
  • Four groups of mice were sensitized to oxazolone by treatment with 4% oxazolone. Three days later two groups were implanted with 0.05 mg indomethacin (Group A) and placebo pellets (Group B). Three days later, right ears of mice in all four groups were challenged with 0.5% oxazolone.
  • One hour post challenge, remaining two groups were treated with buspirone HCl at 50 mg/kg (Group C) or vehicle (Group D). Ear swelling was measured 24 hours after oxazolone challenge.
  • FIG. 12 Effect of systemic buspirone HCl treatment (Group C), indomethacin or placebo treatment (Groups A and B, respectively) on leukocyte infiltration associated with oxazolone induced cutaneous hypersensitivity reaction.
  • These data are from the same mice whose ear thickness measurements are presented in Fig. 11. Biopsies were performed 24 hours after oxazolone challenge.
  • alkyl as used herein, unless otherwise specified, refers to a saturated straight
  • branched, or cyclic hydrocarbon of C 1 to C 20 including methyl, ethyl, propyl, isopropyl, butyl, isobutyl, t-butyl, pentyl, cyclopentyl, isopentyl, neopentyl, hexyl, isohexyl, cyclohexyl, 3- methylpentyl, 2,2-dimethylbutyl, and 2,3- dimethylbutyl.
  • aryl refers to phenyl or
  • substituted phenyl wherein the substituent is independently halo, alkyl, or oxy( alkyl) (for example, methyoxy, ethoxy, etc.), and wherein the aryl can have up to three substituents.
  • heterocycle refers to a cyclic moiety that has O, S, or N in the aromatic ring, including but not limited to, pyrryl, furyl, pyridyl, thiophene, pyrimidyl, thienyl, isothiazolyl, imidazolyl, tetrazolyl, pyrazinyl, pyrimidyl, quinolyl, isoquinolyl, benzothienyl, isobenzofuryl, pyrazolyl, indolyl, purinyl, carbozolyl, and isoxazolyl and the like, optionally substituted with halo (Cl, Br, I, or F), alkyl, oxyalkyl, aryl or oxyaryl.
  • halo Cl, Br, I, or F
  • aralkyl refers to an aryl group with an alkyl substituent.
  • alkaryl refers to an alkyl group that has an aryl substituent.
  • alkene refers to an alkene group of C 2 to C 10 , and specifically includes vinyl, and allyl.
  • the parent buspirone is 8-[4-[4-(2-pyrimidinyl)- 1-piperaziny]butyl]-8-azaspiro-[4.5]decane-7,9- dione, which has the structure illustrated below.
  • buspirone derivative refers to a compound that exhibits an
  • immunosuppressive effect for example, as measured using the assay set out in Example 1, i.e., it suppresses the ear swelling associated with an experimental contact hypersensitivity response by at least 40% at 24 hours after specific antigen challenge, or as evaluated in vivo in humans by the agent's ability to inhibit contact hypersensitivity responses to patch test allergens in patients hypersensitive to a given allergen, using procedures generally accepted by those of skill in the art, and wherein the derivative has the formula:
  • R 1 H; halo (chloro, bromo, fluoro, or iodo);
  • alkyl specifically including CH 3 -, cyclohexyl, (CH 3 ) 2 CH-, CH 3 (CH 2 ) 3 -,
  • R 2 H, C 6 H 5 CH(CH 2 CH 3 )CH 2 -, C 6 H 5 CH(CH 3 ) (CH 2 ) 2 - ,
  • n 1 to 6;
  • X is independently F, Cl, Br, I, OCH 3 , SO 3 -,
  • Ar 1 independently, aryl, (2, 3, or 4-X-C 6 H 4 -) ,
  • toluenesulfonate methylsulfonate, sulfonate, sulfate, phosphate, or carboxylate (such as benzoate, succinate, acetate, glycolate,
  • propionate maleate, malate, citrate, tartrate, ascorbate, benzoate, cinnamoate, mandeloate, benzyloate, and diphenylacetate).
  • buspirone or its derivatives are disclosed in, or can be easily adapted by one of ordinary skill in organic synthesis from procedures disclosed in Wu, et al., J. Med. Chem. 15, 477 (1972), Ger. Patent No.
  • buspirone has significant immunosuppressive
  • buspirone derivatives to act as an immunosuppressant can be conveniently determined by synthesizing the compound and testing it in the biological assay described in Example 1.
  • the active compounds described herein exhibit an immunosuppressive effect when provided topically or systemically.
  • the derivative is considered an immunosuppressant if it suppresses the ear swelling associated with an experimental contact
  • hypersensitivity response by at least 40% at 24 hours after specific antigen challenge.
  • the agent can be evaluated in vivo in humans by assessing the agent's ability to inhibit contact hypersensitivity responses to patch test allergens in patients hypersensitive to a given allergen, using procedures generally accepted by those of skill in the art, or by evaluation in an animal model, for example, of allograft
  • buspirone derivatives of buspirone which are particularly useful are those that have an immunosuppressive effect but which do not exhibit a significant neuroleptic effect, Buspirone derivatives without significant
  • neuroleptic effect can be identified by their ability to bind to serotonin or dopamine receptors, or by assessing their lack of ability to act as a tranquilizer or neuroleptic in mammals, for
  • buspirone and buspirone derivatives are not effective in suppressing contact hypersensitivity.
  • the mechanism of action of buspirone and buspirone derivatives in suppressing the immune response is independent of their serotonin or dopamine receptor blocking properties. Therefore, buspirone derivatives with immunosuppressive effects yet without neuroleptic effects can be provided by the method of selection disclosed generally herein.
  • immunosuppressive compounds with a buspirone nucleus that have a neuroleptic effect can be complexed or modified to eliminate that effect, by one or more of the following processes.
  • Compounds with a buspirone nucleus that exhibit an immunosuppressive effect yet also exhibit a neuroleptic effect can be modified to minimize the neuroleptic effect by decreasing the lipophilicity (equivalent to increasing the hydrophilicity) of the molecule. This can be done by adding one or more charged side chain (s) onto the molecule or by altering the existing side chain to make it more polar. The hydrophilicity of buspirone derivatives will in general increase when charged substituents are added.
  • Another technique for reducing the central nervous system (CNS) effects of compounds that contain a buspirone nucleus is to increase the size of the molecule via a covalent linkage to a large moiety (e.g., albumin or polyethylene glycol), using standard techniques of organic synthesis or by choosing a buspirone derivative with large substituents.
  • a large moiety e.g., albumin or polyethylene glycol
  • a third method for reducing the central nervous system (CNS) effects of a compound that contains a buspirone nucleus includes forming a non-covalent complex of the compound with a cyclic molecule such as a cycloamylose (e.g., a cyclodextrin such as ⁇ -cyclodextrin), which has a spatial arrangement of hydroxyl groups whereby the outer surface of the ring formed by the cycloamylose is hydrophilic and the inner surface is lipophilic.
  • a cyclic molecule such as a cycloamylose (e.g., a cyclodextrin such as ⁇ -cyclodextrin)
  • a cycloamylose e.g., a cyclodextrin such as ⁇ -cyclodextrin
  • guest molecules molecules (or parts thereof), termed “guest molecules”, which are less polar than water and which are of suitable dimensions, to be incorporated into the lipophilic inner cavity, such that the cycloamylose/guest molecule complex presents to the blood-brain barrier as a relatively large and polar compound which is unable to penetrate the barrier.
  • Such complexes may be prepared by any method known to the art, including those described in U.S. Patent No. 4,555,504, which discloses ⁇ -cyclodextrin complexed with digoxin.
  • the central nervous system side effects of a buspirone derivative can be estimated using molecular modeling and/or pharmacophore analysis.
  • the dopamine and serotonin receptors are well characterized and strategies for estimating binding of drugs to these receptors are well established.
  • Buspirone or its above-defined derivative can be administered in the form of a pharmaceutically acceptable quaternary salt.
  • Quaternary salts are typically less lipophilic than the corresponding unquaternized compound, and therefore have a decreased effect on the central nervous system.
  • Nonlimiting examples of quaternary salts that can be used include salts prepared from methyl
  • toluenesulfonate methylsulfonate, sulfonate, phosphate, or carboxylate (such as benzoate, succinate, acetate, glycolate, succinate, maleate, malate, citrate, tartrate, ascorbate, benzoate, cinnamoate, mandeloate, benzyloate, and
  • methyl ammonium tosylate salt of buspirone has been found to be toxic to mice at elevated dosage levels (above 10 mg/kg). Therefore, this quaternary salt of buspirone or its derivatives should be administered as the lowest dosage that achieves a desired effect.
  • Buspirone or its derivative can be included in a pharmaceutically acceptable carrier or diluent in an amount sufficient to deliver to a patient a therapeutic amount of compound in vivo in the absence of serious toxic effects for any of the above described disorders.
  • the concentration of active compound in the drug composition will depend on absorption,
  • dosage values will also vary with the severity of the condition to be alleviated. It is to be further understood that for any particular subject, specific dosage regimens should be adjusted over time according to the individual need and the professional judgment of the person administering or supervising the administration of the compositions, and that the dosage ranges set forth herein are exemplary only and are not intended to limit the scope or practice of the claimed composition.
  • the active ingredient may be administered once, or may be divided into a number of smaller doses to be administered at varying intervals of time.
  • Buspirone or its derivative can be mixed with other active materials which do not impair the desired action, or with materials that supplement the desired action, such as antibiotics,
  • antifungals anti-inflammatories, antivirals, or other immunosuppressive agents.
  • Buspirone or its derivatives can be provided in the form of pharmaceutically-acceptable salts.
  • pharmaceutically-acceptable salts or complexes refers to salts or complexes that retain the desired biological activity of the parent compound and exhibit minimal, if any, undesired toxicological effects.
  • salts are (a) acid addition salts formed with inorganic acids (for example, hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid, and the like), and salts formed with organic acids such as acetic acid, oxalic acid, tartaric acid, succinic acid, malic acid, ascorbic acid, benzoic acid, tannic acid, pamoic acid, alginic acid, polyglutamic acid,
  • inorganic acids for example, hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid, and the like
  • organic acids such as acetic acid, oxalic acid, tartaric acid, succinic acid, malic acid, ascorbic acid, benzoic acid, tannic acid, pamoic acid, alginic acid, polyglutamic acid,
  • naphthalenesulfonic acids naphthalenedisulfonic acids, and polygalacturonic acid
  • base addition salts formed with polyvalent metal cations such as zinc, calcium, bismuth, barium, magnesium,
  • Buspirone or its derivatives can be modified in order to enhance their usefulness as pharmaceutical compositions.
  • various modifications of the active molecule such as alteration of charge, can affect water and lipid solubility and thus alter the potential for percutaneous absorption.
  • vehicle, or carrier can also be modified to enhance cutaneous absorption, enhance the reservoir effect, and minimize potential irritancy or
  • Compounds that are useful are typically those that have a therapeutic index of at least 2, and preferably 5 or 10 or greater, wherein therapeutic index is defined as EC 50 /IC 50 .
  • Mammals, and specifically humans, suffering from pathological cutaneous, ocular, or mucosal immune responses can be treated by topical administration to the patient of an effective amount of buspirone or its derivative or its salt, optionally in combination with a pharmaceutically acceptable carrier or diluent.
  • the active compound is included in the pharmaceutically acceptable topical carrier or diluent in an amount sufficient to deliver to a patient a therapeutic amount of the buspirone derivative locally in the absence of serious toxic effects.
  • local immunosuppression can be achieved by topically administering lower doses of buspirone derivatives than would be required if the agents were administered systemically.
  • Typical dosages for topical application for all of the above-identified conditions are those ranging from 0.001 to 100% by weight of the active compound.
  • Solutions or suspensions for topical application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose.
  • the pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide.
  • Suitable vehicles or carriers for topical application can be prepared by conventional techniques, such as lotions, suspensions,
  • thickening agents examples include petrolatum, beeswax, xanthan gum, or polyethylene, humectants such as sorbitol, emollients such as mineral oil, lanolin and its derivatives, or squalene.
  • humectants such as sorbitol
  • emollients such as mineral oil, lanolin and its derivatives, or squalene.
  • solutions and ointments are commercially available, especially for ophthalmic applications.
  • Thickening agents, emollients, and stabilizers can be used to prepare topical compositions.
  • thickening agents examples include petrolatum, beeswax, xanthan gum, or polyethylene glycol, humectants such as sorbitol, emollients such as mineral oil, lanolin and its derivatives, or squalene.
  • humectants such as sorbitol
  • emollients such as mineral oil, lanolin and its derivatives, or squalene.
  • Natural or artificial flavorings or sweeteners can be added to enhance the taste of topical preparations applied for local effect to mucosal surfaces.
  • Inert dyes or colors can be added, particularly in the case of preparations designed for application to oral mucosal surfaces.
  • Buspirone or its derivative can be applied in a time release formulation via transdermal patches or by slow release polymers.
  • the active compounds can be prepared with carriers that will protect the compound against rapid release, such as a
  • controlled release formulation including implants and microencapsulated delivery systems.
  • Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Many methods for the preparation of such formulations are patented or generally known to those skilled in the art.
  • Mammals, and specifically humans, suffering from pathogenic immune responses can also be treated by the systemic administration to the patient of an effective amount of the buspirone derivative or its salt optionally in combination with a
  • the buspirone derivative can be administered, for example, subcutaneously, intravenously, intraperitoneally, intramuscularly, parenterally, orally, submucosally, by inhalation, or
  • Typical systemic dosages for all of the above-identified conditions are those ranging from 20 mg/kg to 0.0001 mg/kg per day as a single daily dose or divided daily doses.
  • the effective dosage of the parent compound, buspirone, for systemic immunosuppression is believed to be higher than the effective dosage of buspirone for inducing a neuroleptic effect.
  • the buspirone derivative is administered
  • Oral compositions will generally include an inert diluent or an edible carrier. They may be enclosed in gelatin capsules or compressed into tablets.
  • the active compound can be incorporated with excipients and used in the form of tablets, troches, or capsules. Pharmaceutically compatible binding agents, and/or adjuvant materials can be included as part of the composition.
  • the tablets, pills, capsules, troches and the like can contain any of the following ingredients, or compounds of a similar nature: a binder such as microcrystalline cellulose, gum tragacanth or gelatin; an excipient such as starch or lactose, a disintegrating agent such as alginic acid,
  • Primogel, or corn starch a lubricant such as magnesium stearate or Sterotes; a glidant such as colloidal silicon dioxide; a sweetening agent such as sucrose or saccharin; or a flavoring agent such as peppermint, methyl salicylate, or orange
  • dosage unit form When the dosage unit form is a capsule, it can contain, in addition to material of the above type, a liquid carrier such as a fatty oil. In addition, dosage unit forms can contain various other
  • materials which modify the physical form of the dosage unit for example, coatings of sugar, shellac, or other enteric agents.
  • buspirone derivative or its salts can be administered as a component of an elixir
  • a syrup may contain, in addition to the active compounds, sucrose as a sweetening agent and certain preservatives, dyes and colorings and flavors.
  • Solutions or suspensions used for parenteral, intradermal, or subcutaneous, application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide.
  • the parenteral preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic.
  • bacteriostatic water physiological saline
  • Cremophor EL TM BASF, Parsippany, NJ
  • PBS phosphate buffered saline
  • the active compounds are prepared with carriers that will protect the compound against rapid elimination from the body on systemic delivery, such as a controlled release formulationo including implants and
  • Biodegradable, biocompatible polymers can be used, such as
  • polyglycolic acid collagen, polyorthoesters, and polylactic acid. Methods for preparation of such formulations will be apparent to those skilled in the art.
  • Liposomal suspensions including liposomes targeted to infected cells with monoclonal
  • antibodies to specific antigens can also be used as pharmaceutically acceptable carriers. These may be prepared according to methods known to those skilled in the art, for example, as described in
  • liposome formulations may be prepared by dissolving appropriate lipid(s) (such as stearoyl phosphatidyl ethanolamine, stearoyl phosphatidyl choline, arachadoyl phosphatidyl choline, and cholesterol) in an organic solvent that is then evaporated, leaving behind a thin film of dried lipid on the surface of the container. An aqueous solution of the buspirone derivative is then introduced into the container. The container is then swirled by hand to free lipid material from the sides of the container and to disperse lipid aggregates, thereby forming the liposomal suspension.
  • appropriate lipid(s) such as stearoyl phosphatidyl ethanolamine, stearoyl phosphatidyl choline, arachadoyl phosphatidyl choline, and cholesterol
  • Buspirone and buspirone derivatives are capable of acting systemically or topically to suppress the immune response in humans and other mammals.
  • the compounds, or therapeutic compositions thereof are useful for the treatment of a myriad of immunological disorders or other pathological conditions associated with an immune response.
  • disorders include cutaneous contact hypersensitivity, host rejection of foreign organ or tissue transplants; graft-vs-host disease in which donor immunological cells present in the graft attack host tissues in the recipient of the graft; diseases with proven or possible autoimmune components, such as rheumatoid arthritis and juvenile rheumatoid arthritis, aphthous ulcer, lichen planus, psoriatic arthritis, psoriasis, excema, conjunctivitis, Sjogren's Syndrome, including keratoconjunctivitis sicca secondary to Sjogren's Syndrome, ulceris, alopecia areata, cutaneous lupus erythematosus, scleroderma, vaginitis, proctitis, drug eruptions, leprosy reversal reactions, erythema nodosum leprosum, autoimmune uveitis, multiple sclerosis, allergic encephalomyelitis, systemic lupus ery
  • dermatitis and hypersensitivity to pollen, insect stings and certain foods can also be useful in reducing the infiltration of skin by malignant leukocytes in diseases such as mycosis fungoides.
  • Buspirone and its derivatives can also be used to increase tear production in a patient suffering from deficient tears in the eye due to an
  • KCS immune mediated keratoconjunctivitis
  • corneal and conjunctival lesions ensues from the dry eye state.
  • Buspirone or its active derivatives can be provided as an ophthalmic drop or ophthalmic ointment to humans or other mammals, including dogs and cats, in an effective amount in a suitable vehicle.
  • This topical ophthalmic treatment can also serve to correct corneal and conjunctival disorders exacerbated by tear deficiency and KCS, such as corneal scarring, corneal ulceration, filamentary keratitis,
  • Buspirone and its derivatives can also be used to decrease immune responses which contribute to granulation and neovascularation in the cornea.
  • Buspirone HCl was used for the procedure in Example 1 as a model of an active immunosuppressant.
  • Buspirone derivatives can be measured against this model, and are considered active if they suppress the swelling response by at least 40% 24 hours after specific antigen
  • buspirone significantly suppressed the tissue swelling associated with the elicitation phase of contact hypersensitivity to oxazolone.
  • mice treated topically with buspirone HCl unlike those treated systemically, exhibited no drowsiness or other evidence of central nervous system
  • Buspirone expresses both serotonin and dopamine receptor antagonist activity. However, unlike buspirone, it was discovered that the chemically unrelated serotonin antagonists, trazadone and mianserin, and the dopamine receptor antagonist, haloperidol, were not effective in suppressing contact hypersensitivity. On the basis of this, it appears that the mechanism of action of buspirone on the immune response is independent of its serotonin or dopamine receptor blocking properties, and therefore, buspirone derivatives with
  • Buspirone HCl, mianserin, trazadone, haloperidol and oxazolone were purchased from the Sigma
  • mice hypersensitivity were performed as follows. The abdomens of the mice were shaved with electric clippers, 50 ⁇ l of a 4% (w/w) solution of oxazolone in 4 : 1 (v:v) acetone: olive oil were applied to the shaved abdomen, and 5 ⁇ l of the same solution were applied to each hind footpad. Five to eight days later, the mice were challenged for contact
  • mice were treated subcutaneously with buspirone HCl 500 or 50 mg/kg body weight) in 0.1 mL of carrier (Cremophor EL, BASF, Parsippany, NJ), or with 0.1 mL of carrier alone.
  • carrier Carrier
  • mice were treated in a similar fashion with 50 mg/kg body weight of trazadone, mianserin, haloperidol, or buspirone HCl in 1 mL olive oil or with olive oil alone.
  • Topical Buspirone HCl Treatment For these experiments, both ears of each mouse were
  • mice Two hours before, or twenty-four hours after application of hapten, the right ears of some mice were treated with buspirone HCl in vehicle, applied epicutaneously to both surfaces. The right ears of control mice were similarly treated, but with vehicle alone. In the case of experiments designed to assess topical effects on the sensitization phase, only the right ear is challenged. (See Figures 9 and 10)
  • ear thicknesses were determined with an engineer's micrometer.
  • the increment (delta) in ear thickness (ear swelling) was calculated as the 24- or 48-hour value minus the baseline (pre-challenge) value and expressed in units of 10 -4 inches. Mice were killed by cervical dislocation after the measurement of 24-hour ear thickness was obtained, and the ears were processed for histologic examination.
  • FIGS. 1 and 2 illustrate the effect of topical application of 100 mg/mL of buspirone HCl (Group B) or carrier alone (Group A) on expression of contact
  • buspirone HCl significantly decreased ear swelling ( Figure 1) and aggregation of leukocytes (Figure 2).
  • HCl was extremely effective in diminishing both the tissue swelling and the leukocyte infiltration associated with contact hypersensitivity reactions, these effects were observed in the absence of detectable alterations in the behavior of the mice.
  • mice treated topically with buspirone HCl appeared active and retained apparently normal interest in food and water.
  • Serotonin or Dopamine Receptor Antagonists systemic buspirone was compared to the serotonin receptor antagonists, trazadone or mianserin, and to the dopamine receptor antagonist, haloperidol, for their ability to inhibit cutaneous contact hypersensitivity. At a dose of 50 mg/kg, only buspirone HCl significantly reduced cutaneous contact hypersensitivity ( Figures 3 and 4).
  • buspirone HCl leukocyte infiltration associated with the response in mice treated with 500 or 50 mg/kg buspirone HCl was also diminished compared to responses in mice not treated with the drug ( Figure 8).
  • buspirone HCl also produced other remarkable systemic effects.
  • mice were sensitized to oxazolone as described in Example 1. Three days later, slow release indomethacin pellets (0.05 mg, 3 week release) were implanted subcutaneously under light ether
  • mice completely block prostaglandin synthesis in mice, by Jun, D.D., et al., J. Invest. Dermatol. 90:311 (1988). Three days later, mice were challenged for contact hypersensitivity as in Example 1. When the hypersensitivity response was assessed 24 hours later, indomethacin was shown to have no
  • Buspirone derivatives which lack serotonin receptor binding or dopamine receptor binding activity can be identified as follows.
  • radiolabeled ligand known to bind serotonin and/or dopamine receptors can be bound to an appropriate substrate expressing one or both of these
  • radiolabeled quipazine which is available commercially can be used as the ligand.
  • the buspirone derivative to be tested is then incubated with the radiolabeled quipazine ligand combination. Displacement of radiolabeled ligand is positive evidence that the buspirone derivative being tested can bind serotonin and/or dopamine receptors.
  • the amount of radiolabeled ligand which is displaced is determined by an appropriate standard curve which can also provide information concerning binding affinities.
  • the displaced radiolabeled ligand can be quantitated using a standard scintillation counter.
  • Binding studies using 3 H-quipazine are described in detail by Milburn, CM. and Peroutka, S.J., J. Neurochem. 52:1787-1792 (1989). Briefly, rat cortices are homogenized in 20 volumes of 50 mM Tris HCl buffer pH 7.7 at 25 °C and centrifuged at
  • the pellet is resuspended in fresh buffer and incubated at 37°C for 10 min. After the final centrifugation, the pellet is resuspended in 80 volumes of Krebs-HEPES buffer (25 mM HEPES, 118 mM NaCl, 5 mM KCl, 2.5 mM CaCl 2 , and
  • Tissue (10 mg of original wet weight) is added to assay tubes containing 0.8 nM [ 3 H] quipazine and displacing drug or buffer in a final volume of 1 mL.
  • Non-specific binding is defined using 1 micromole zacopride.
  • affinities for dopamine and/or serotonin receptors of one/tenth or less than native buspirone are considered to be potentially useful as systemic immunosuppressants if they are at least 50% as active as native buspirone on a weight basis in suppressing immunologically specific responses such as contact hypersensitivity.

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Abstract

A method for the treatment of a human or other mammal in need of immunosuppression is disclosed in which the mammal is treated topically or systemically with an effective amount of buspirone or a buspirone derivative, in a pharmaceutically-acceptable diluent or carrier for topical or systemic administration.

Description

TOPICAL AND SYSTEMIC APPLICATION OF BUSPIRONE OR
DERIVATIVES THEREOF FOR TREATMENT OF PATHOLOGICAL
CONDITIONS ASSOCIATED WITH IMMUNE RESPONSES
Background of the Invention This application is in the area of the topical and systemic administration of buspirone or derivatives thereof for the treatment of
pathological conditions associated with immune responses.
The immune system specifically recognizes and selectively eliminates foreign invaders, or other antigenic agents, by a process known as the immune response. The immune response has three major characteristics: it responds adaptively to foreign invaders, it exhibits strong specificity, and it displays a long-term memory of earlier contacts with specific foreign pathogens or antigens. The immune response involves the production of
antibodies and/or the destruction of antigenic cells by T lymphocytes; both the antibodies and the
T lymphocytes are highly specific for the antigen or hapten.
When directed against an infectious organism, the immune response can provide great benefit to the host. As an example, an important component of current public health practices is the use of vaccines to elicit immune responses against infectious organisms that cause severe illness and death. However, when directed against agents that are relatively innocuous, such as pollen, animal dander, and certain plant resins, the cells, antibodies, and mediators which represent the effector components of the immune response can cause damage to the host's tissues that is out of proportion to any threat to health posed by the antigenic agent that first elicited the response. Topical disorders that involve the immune system can result in result in one or more of the
following symptoms cr signs: itching, swelling, redness, blisters, crusting, ulceration, pain, scaling, cracking, hair loss, scarring, or oozing of fluid involving the skin, eye, or mucosal membranes.
Cutaneous contact hypersensitivity responses are complex expressions of cellular immunity
characterized by antigen-dependent changes in lymphocyte traffic, the recruitment of circulating leukocytes to the site of antigen challenge
(leukocyte infiltration), and alterations in vascular permeability and blood flow resulting in tissue swelling (edema). While T cells are required for the expression and immunological specificity of the response, many other cell types also have roles in the reaction, including
Langerhans' cells, keratinocytes, and vascular endothelial cells. Antigen presentation is thought to be effected primarily by Langerhans' cells, whereas much of the local expression of the response is thought to be regulated by cytokines derived from both T cells and accessory cells.
Pharmacological studies have indicated that a number of mediators in addition to cytokines may contribute to the expression of contact
hypersensitivity and other forms of cell-mediated immunity. There has been particular interest in the role of serotonin (5-hydroxytryptamine, 5-HT) in these reactions. For example, serotonin has been shown to have a wide range of actions on T cells and other effector cells in vitro or in vivo , and pharmacological agents that deplete or
antagonize serotonin can diminish expression of cell-mediated immunity. Early studies raised the possibility that such agents might reduce cell- mediated immunity by antagonizing or depleting mast cell-associated serotonin. However, more recent findings indicate that at least one of these drugs, reserpine, can inhibit contact hypersensitivity independently of mast cells, probably through direct effects on T cells.
In humans and companion animals, cutaneous contact hypersensitivity responses can occur on exposure to certain plant resins, such as those of poison ivy, and other commonly encountered agents in the environment. In individuals sensitized to such commonly encountered agents, a severe contact reaction can result upon exposure, with significant associated morbidity. Severe or repeated contact hypersensitivity reactions can be followed by significant chronic changes, such as scarring of affected tissues, itchiness, swelling, scaling and oozing of tissue fluid through the skin surface. This pathology may predispose the patient to bacterial superinfection. In the eye, chronic immune responses can lead to diminished vision or actual blindness. In the lung, chronic immune responses, such as chronic allergic asthma, can result in serious chronic lung disease.
Examples of pathological or undesired immune responses other than cutaneous contact
hypersensitivity, including systemic disorders, include but are not limited to host rejection of foreign organ or tissue transplants; graft-vs-host disease in which donor immunological cells present in the graft attack host tissues in the recipient of the graft; diseases with proven or possible autoimmune components, such as rheumatoid arthritis and juvenile rheumatoid arthritis, aphthous ulcer, lichen planus, psoriatic arthritis, psoriasis, excema, conjunctivitis, Sjogren's Syndrome, including keratoconjunctivitis sicca secondary to Sjogren's Syndrome, iritis, alopecia areata, cutaneous lupus erythematosus, scleroderma, vaginitis, proctitis, drug eruptions, leprosy reversal reactions, erythema nodosum leprosum, autoimmune uveitis, multiple sclerosis, allergic encephalomyelitis, systemic lupus erythematosis, acute necrotizing hemorrhagic encephalopathy, idiopathic bilateral progressive sensorineural hearing loss, aplastic anemia, pure red cell anemia, idiopathic thrombocytopenia,
polychondritis, scleroderma, Wegener's
granulomatosis, chronic active hepatitis,
myasthenia gravis, Stevens-Johnson syndrome, idiopathic sprue, Crohn's disease, ulcerative colitis, Graves ophthalmopathy, sarcoidosis, primary biliary cirrhosis, primary juvenile diabetes, uveitis posterior, and interstitial lung fibrosis; asthma; allergic asthma, allergic responses due to arthropod bite reactions, and inappropriate allergic responses to other
environmental stimuli such as atopic dermatitis and hypersensitivity to pollen, insect stings and certain foods.
Various therapeutics have been utilized as immunosuppressants including steroid hormones, antiproliferatives such as methotrexate and azathioprine, cyclosporine, alkylating agents such as cyclophosamide and busulfan, psoralen plus ultraviolet A (PUVA), and antibiotics.
Corticosteroids, when administered systemically, can be effective but can be associated with
significant and potentially dangerous side effects. Topically applied corticosteroids have some efficacy in treating these conditions, but are only partially effective in many instances and have their own significant side effects, including atrophy of tissue, formation of telangiectasia, blanching, and a myriad of systemic effects if significantly absorbed. As a result, there still remains a strong need to provide new
immunosuppressive agents that can minimize or prevent pathological immune responses.
In contrast to the immune response, an
inflammatory response is a pathologic condition that can occur in response to immunologically non- specific injury, either from physical (such as trauma), chemical, or biological agents. An inflammatory response is characterized by increased blood flow and redness in the inflamed area, increased capillary permeability and edema, and recruitment of immunologically non-specific white blood cells, especially neutrophils, that remove injurious material and promote repair. Unlike immune responses, inflammatory responses do not respond adaptively to the inciting stimulus, do not show specificity and do not exhibit long term memory. Cellular products of lymphocytes may contribute to or induce an inflammatory response. However, because of the differences in mechanisms, a compound can function as an anti-inflammatory agent without having immunosuppressive properties. Phenylbutazone, indomethacin, aspirin, ibuprofen, and acetaminophen are examples of anti-inflammatory compounds which have no significant
immunosuppressive activity, as demonstrated by their lack of a significant effect on
immunologically mediated responses, such as contact hypersensitivity.
PCT International Publication No. WO 91/02527 discloses a method and composition to treat cutaneous, mucosal, or ocular hypersensitivity that includes administering an effective amount of reserpine, spiperone, or other serotonin
antagonist.
It is an object of the present invention to present a method for the topical treatment of cutaneous, mucosal and ocular pathologies
associated with immune responses.
It is yet another object of the present
invention to present a method for the systemic treatment of pathogenic conditions associated with immune responses.
Summary of the Invention
A method for the treatment of a human or other mammal in need of immunosuppression is disclosed in which the mammal is treated topically or
systemically with an effective amount of buspirone or a buspirone derivative, in a pharmaceutically- acceptable diluent or carrier for topical or systemic administration.
Buspirone and its active derivatives can be administered as general immunosuppressive agents to treat a variety of disorders. In one embodiment, a method is provided for the treatment of a
cutaneous, ocular, or mucosal condition in a human or other mammal resulting from pathology associated with an immune response that includes topical application of an effective amount of buspirone or a buspirone derivative or its pharmaceutically acceptable salt, in a pharmaceutically-acceptable diluent or carrier for topical application. The compounds are useful as topical agents in treating contact dermatitis, atopic dermatitis, eczematous dermatitis, psoriasis, Sjogren's Syndrome,
including keratoconjunctivitis sicca secondary to Sjogren's Syndrome, alopecia areata, allergic responses due to arthropod bite reactions, Crohn's disease, aphthous ulcer, iritis, conjunctivitis, keratoconjunctivitis, ulcerative colitis, asthma, allergic asthma, cutaneous lupus erythematosus, scleroderma, vaginitis, proctitis, and drug eruptions. The compounds may also be useful in reducing the infiltration of skin by malignant leukocytes in diseases such as mycosis fungoides. These compounds are also effective in treating an aqueous-deficient dry eye state (such as immune mediated keratoconjunctivitis) in a patient suffering therefrom, by administering the compound topically to the eye.
In a second embodiment, buspirone or its derivative is administered systemically for the treatment of systemic or topical disorders involving the immune system. The parent buspirone can have a neuroleptic effect when administered systemically (but not typically when administered topically), however, it is a model of an active immunosuppressant. Derivatives of buspirone are considered to be immunosuppressants if they suppress the ear swelling associated with an experimental contact hypersensitivity response by at least 40% at 24 hours after specific antigen challenge.
Brief Description of the Figures
Figure 1 - Effect of topically administered buspirone HCl on tissue swelling associated with oxazolone-induced contact hypersensitivity
reactions. Oxazolone (10 μl of a 0.5% (w:w) solution) was applied to both ears of all mice and the change in ear thickness was measured at a specified interval thereafter. Buspirone HCl (100 mg/ml) (Group B) or vehicle alone (Group A) was applied to the right ear of Balb/c mice 2 hours after challenge. The change in ear thickness (post-challenge value minus baseline pre-challenge value) was measured 24 hours after oxazolone challenge. The data are presented as the mean ± SEM (standard error of the mean). The reduction in ear swelling observed with 100 mg/ml buspirone HCl was significant when compared to the reactions observed in the vehicle treated animals (Group A) (**=p<0.01).
Figure 2 - Effects of topical treatment with 100 mg/kg buspirone HCl on leukocyte infiltration associated with 24-hour contact hypersensitivity reactions. These data (mean ± SEM) are derived from the same mice whose ear thickness values are shown in Figure 1. The reduction in leukocyte infiltration observed in animals treated with 100 mg/kg buspirone HCl was significant when compared to the reactions observed in animals treated with vehicle alone (* =p<0.05).
Figure 3 - Comparative effects of 50 mg/kg subcutaneous administration of mianserin HCl (Group A) , trazadone HCl (Group B), haloperidol (Group C), buspirone HCl (Group D), and vehicle (Group E) on the tissue swelling associated with oxazolone- induced cutaneous contact hypersensitivity
reactions. Buspirone HCl, the other agents, or vehicle alone were administered to BALB/c mice 1 hour after right ears only were challenge for contact hypersensitivity. The change in ear thickness (post-challenged value minus baseline pre-challenge value) was measured 24 hours after oxazolone challenge. The data are presented as the mean ± SEM. The reduction in ear swelling observed with buspirone HCl was significant when compared to the reactions observed in the challenged right ears of the control, vehicle (Group E, olive oil) treated animals (**=p<0.01), whereas haloperidol. trazadone and mianserin did not significantly suppress the tissue swelling associated with contact hypersensitivity.
Figure 4 - Comparative effects of subcutaneous administration of 50 mg/kg mianserin HCl (Group A), trazadone HCl (Group B), haloperidol (Group C), buspirone HCl (Group D), and systemic vehicle
(Group E) on leukocyte infiltration associated with 24-hour contact hypersensitivity reactions. These data (mean ± SEM) are derived from the same mice whose ear thickness values are shown in Figure 3. The reduction in leukocyte infiltration observed in the right (oxazolone-challenged) ears of animals treated with buspirone HCl was significant when compared to the reactions observed in animals treated with vehicle alone (*=p<0.05), while haloperidol, trazadone and mianserin did not significantly suppress the leukocyte infiltration associated with contact hypersensitivity.
Figure 5a,b - Effect of topically administered buspirone HCl on tissue swelling associated with oxazolone-induced contact hypersensitivity
reactions. Oxazolone was applied to both ears of all mice at different times either pre- or post- buspirone HCl treatment and the change in ear thickness was measured at a specified interval thereafter. a. Two hours before oxazolone
challenge, 100 mg/ml buspirone HCl in Vehicle-N was applied to both surfaces of the right ears of Group A mice, whereas vehicle alone (0% buspirone HCl) was applied to both surfaces of the right ears of the control, vehicle only (Group B Vehicle-N) animals. The ears were measured 24 hours after oxazolone challenge. Local prechallenge treatment of the right ear with buspirone HCl significantly suppressed tissue swelling in the treated ear
(**p<0.01 vs contralateral oxazolone treated ears or vs right ears of vehicle treated group) .
Treatment of the right ear with 100 mg/ml buspirone HCl had no significant effect on the magnitude of swelling in the contralateral oxazolone treated ear. b. In a separate experiment, twenty-four hours after oxazolone challenge, 100 mg/ml
buspirone HCl in Vehicle-N was applied to both surfaces of the right ears of Group B mice, whereas vehicle alone (0% buspirone HCl) was applied to both surfaces of the ears of control vehicle only
(Group A vehicle-N) mice. The change in ear thickness was determined 24 hours after treatment with buspirone HCl, i.e. at 48 hours after
challenge with oxazolone. Treatment with buspirone HCl significantly diminished contact
hypersensitivity reactions in the right ears of the treated animals (**=p<0.01 when compared to the right ears in the control mice (Group A), and p<0.05 when compared to the contralateral ears of the same mice) . The reactions in the left ears of the mice treated on the right ears with buspirone HCl (Group B) were not reduced when compared to reactions in the left ears of the vehicle-treated mice (Group A).
Figure 6a, b - Effect of topical treatment with buspirone HCl on leukocyte infiltration associated with oxazolone-induced contact hypersensitivity reactions. These data (mean ± SEM) are from the same mice whose ear thickness measurements are presented in Figure 5 a,b. Biopsies were performed
24 hours (a), or 48 hours (b) after application of oxazolone. Topical prechallenge treatment with buspirone HCl significantly diminished the
reactions when compared to those in vehicle-treated mice (6a), as did topical postchallenge treatment with buspirone HCl (6b) (**=p<0.01 in both 6a and b). Figure 7 - Effect of systemic buspirone HCl (Group A, 500 mg/kg; Group B, 50 mg/kg)
administered subcutaneously on tissue swelling associated with oxazolone induced cutaneous hypersensitivity reactions of the right ear.
Buspirone HCl (Groups A and B) or vehicle alone (Group C) was administered to Balb/c mice 1 hour after challenge. Change in ear thickness (postchallenge - baseline pre-challenge value) was measured 24 hours after oxazolone challenge. The data are presented as ±SEM. Systemic treatment with buspirone HCl (at 500 or 50 mg/kg)
significantly reduced the tissue swelling
associated with contact hypersensitivity compared to the responses observed in the right ears of mice treated with vehicle alone (Group C) (**=p<0.01).
Figure 8 - Effect of systemic buspirone HCl (500 or 50 mg/kg buspirone, administered subcutaneously) on leukocyte infiltration associated with oxazolone induced cutaneous hypersensitivity reactions of the right ear. These data are derived from the same mice whose ear thickness values are shown in Figure 7. Systemic treatment with buspirone HCl (at 500 or 50 mg/kg) significantly reduced the leukocyte infiltration when compared to the reaction observed in animals treated with vehicle alone (**=p<0.01 for both Group A and Group B) .
Figure 9 - The effect of topical treatment with buspirone HCl on suppression of the sensitization phase of oxazolone challenge. Buspirone HCl at 100 mg/ml (Group A) or vehicle-N (Group B) was applied to abdomen of mice 3 days prior to sensitization of Balb/c mice with 4% oxazolone. This treatment was repeated 3 days after sensitization. The right ears of all mice were then challenged with 0.5% oxazolone. The change in the ear thickness was measured 24 hours after oxazolone challenge. The data are presented as the mean ± SEM. The
reduction in ear swelling observed with buspirone HCl treatment (Group A) was significant when compared to those treated with vehicle only (Group B). (**=p<0.01).
Figure 10 - Effect of topical treatment with buspirone HCl (100 mg/ml) on leukocyte infiltration associated with suppression of sensitization phase of oxazolone challenge. These data are from the same mice whose ear thickness measurements are presented in Fig. 9. Biopsies were performed 24 hours after oxazolone challenge. Topical buspirone HCl, administered both pre- and post-sensitization (Group A), significantly diminished the reactions as compared to the vehicle-only treated mice (Group
B). (**=p<0.01).
Figure 11 - Effect of systemic buspirone HCl treatment (50 mg/kg, administered subcutaneously), indomethacin, or placebo pellets (0.05 mg/pellet, implanted subcutaneously) on oxazolone induced contact hypersensitivity. Four groups of mice were sensitized to oxazolone by treatment with 4% oxazolone. Three days later two groups were implanted with 0.05 mg indomethacin (Group A) and placebo pellets (Group B). Three days later, right ears of mice in all four groups were challenged with 0.5% oxazolone. One hour post challenge, remaining two groups were treated with buspirone HCl at 50 mg/kg (Group C) or vehicle (Group D). Ear swelling was measured 24 hours after oxazolone challenge. Pre-challenge treatment with
indomethacin or placebo (Groups A and B,
respectively) did not have a significant effect on the hypersensitivity response as compared to control (Group D) in which vehicle alone was administered post-challenge. However, buspirone HCl treatment (Group C), applied post-challenge. significantly reduced ear swelling as compared to the control group (Group D, vehicle only).
(**=p<0.01).
Figure 12 - Effect of systemic buspirone HCl treatment (Group C), indomethacin or placebo treatment (Groups A and B, respectively) on leukocyte infiltration associated with oxazolone induced cutaneous hypersensitivity reaction. These data are from the same mice whose ear thickness measurements are presented in Fig. 11. Biopsies were performed 24 hours after oxazolone challenge. Buspirone HCl treatment (Group C) significantly reduced leukocyte infiltration as compared to the control group (Group D, vehicle only) (**=p<0.01); whereas indomethacin and placebo treatment had no significant effect, when compared to control groups.
Detailed Description of the Invention The term alkyl, as used herein, unless otherwise specified, refers to a saturated straight,
branched, or cyclic hydrocarbon of C1 to C20, including methyl, ethyl, propyl, isopropyl, butyl, isobutyl, t-butyl, pentyl, cyclopentyl, isopentyl, neopentyl, hexyl, isohexyl, cyclohexyl, 3- methylpentyl, 2,2-dimethylbutyl, and 2,3- dimethylbutyl.
The term aryl, as used herein, and unless otherwise specified, refers to phenyl or
substituted phenyl, wherein the substituent is independently halo, alkyl, or oxy( alkyl) (for example, methyoxy, ethoxy, etc.), and wherein the aryl can have up to three substituents.
The term heterocycle refers to a cyclic moiety that has O, S, or N in the aromatic ring, including but not limited to, pyrryl, furyl, pyridyl, thiophene, pyrimidyl, thienyl, isothiazolyl, imidazolyl, tetrazolyl, pyrazinyl, pyrimidyl, quinolyl, isoquinolyl, benzothienyl, isobenzofuryl, pyrazolyl, indolyl, purinyl, carbozolyl, and isoxazolyl and the like, optionally substituted with halo (Cl, Br, I, or F), alkyl, oxyalkyl, aryl or oxyaryl.
The term aralkyl refers to an aryl group with an alkyl substituent.
The term alkaryl refers to an alkyl group that has an aryl substituent.
The term alkene, as referred to herein, and unless otherwise specified, refers to an alkene group of C2 to C10, and specifically includes vinyl, and allyl.
I. Structure and Synthesis of Buspirone Derivatives
The parent buspirone is 8-[4-[4-(2-pyrimidinyl)- 1-piperaziny]butyl]-8-azaspiro-[4.5]decane-7,9- dione, which has the structure illustrated below.
Figure imgf000016_0001
The term "buspirone derivative" as used herein refers to a compound that exhibits an
immunosuppressive effect, for example, as measured using the assay set out in Example 1, i.e., it suppresses the ear swelling associated with an experimental contact hypersensitivity response by at least 40% at 24 hours after specific antigen challenge, or as evaluated in vivo in humans by the agent's ability to inhibit contact hypersensitivity responses to patch test allergens in patients hypersensitive to a given allergen, using procedures generally accepted by those of skill in the art, and wherein the derivative has the formula:
wherein:
Figure imgf000017_0001
R1 = H; halo (chloro, bromo, fluoro, or iodo);
alkyl, specifically including CH3-, cyclohexyl, (CH3)2CH-, CH3(CH2)3-,
(CH3)2CHCH2-, CH3CH2CH(CH3)-, (CH3)3C-, and
-CH3(CH2)p; Y-CH2(CH2)n-; oxyalkyl; or aryl, specifically including C6H5-, (2, 3, or 4)-
(OCH3)C6H4- and (2, 3, or 4) -(CH3) C6H4-;
2-X-C6H4-, 3-X-C6H4-, or 4-X-C6H4-; oxyaryl; or alkaryl;
R2 = H, C6H5CH(CH2CH3)CH2-, C6H5CH(CH3) (CH2) 2- ,
C6H5CHTCH (CH3) CH2-, C6H5CH2CH2CH (CH3)-,
C6H5CH(CH3) (CH2)3-,
(2, 3, or 4)-(alkyl)-C6H4CH(CH3) (CH2)3-,
(2, 3, or 4)-(alkyloxy)-C6H4CH(CH3) (CH2)3,
(2, 3, or 4)-X-C6H4-alkyl, specifically including
(2, 3, or 4)-X-C6H4CH(CH2CH3)CH2-,
(2, 3, or 4)-X-C6H4CH(CH3) (CH2)-,
4-X-C6H4CH(CH3) (CH2)2-, and
4-X-C6H4-CH(CH3) (CH2)3-; C6H5CH(OCH3) (CH2)2-,
C6H5CH--CH-CH2-, C6H5CO(CH2)3-, C6H5CO (CH2)4-,
Figure imgf000017_0002
(2, 3, or 4)-(alkyl)-C6H4CO(CH2)3-,
(2, 3, or 4)-(alkyl-oxy)-C6H4CO(CH2)3-,
(2, 3, or 4)-X-C6H4CO(CH2)n-,
2-thienyl-CO-(CH2)3-,
-alkyl-piperazinyl-aryl;
-alkyl-C3-8cycloalkyl-aryl;
-alkyl-pip-razinyl-heterocycle; -alkyl-C3-8cycloalkyl-heterocycle;
-alkyl-C3-8cycloalkyl-Ar1;
-alkyl-piperazinyl-Ar1;
-alkenyl-piperazinyl-aryl;
-alkenyl-C3-8cycloalkyl-aryl;
-alkyl-aryl-heterocycle;
-alkyl-heterocycle-aryl;
-alkenyl-C3-8cycloalkyl-Ar1;
-alkenyl-piperazinyl-heterocycle; -alkenyl-C3-8cycloalkyl-heterocycle;
-alkenyl-piperazinyl-Ar1;
Ar1-CH(CH2)n-,
Ar
Figure imgf000018_0001
1
(2, 3, or 4)-X-C6H4C(CH3)CH(CH2)2-, where the conformation about the double bond is cis or trans,
(2, 3, or 4)-X-C6H4C(CH3)CHCH2-, where the conformation about the double bond is cis or trans,
(2, 3, or 4)-X-C6H4COCH=CHCH2-,
Y-CH2(CH2)n-, Ar1(CH2)n-, C1 to C20 alkyl,
X-(CH2)nCO-, or X-(CH2)n-;
R3 = =O, =NH, =S, chloro, bromo, iodo, fluoro, alkyl, or aryl;
n = 1 to 6;
p = 1 to 20;
X = is independently F, Cl, Br, I, OCH3, SO3-,
NH2, H, -OH, -COOH, -COOR, -SO3H, -CN,
-NHSO3H, -NO,, or -SO2NH2;
Y = H, F, Cl, Br, I, -SO3, -PO4 =, -OH, -SH,
-SCH3, -CH3SO2-, -NH2, or -CO2-; and
Ar1 = independently, aryl, (2, 3, or 4-X-C6H4-) ,
(2, 3, or 4)-(CH2X)C6H4-,
(2, 3, or 4)-(CX3)C6H4-,
(2, 3, or 4) - (CHX2) C6H4-, 2-thienyl, or
(2, 3, or 4)-X-C6H4CH2-; or its pharmaceutically acceptable salt, including any quaternary salt known to those in the art, and specifically including the quaternary ammonium salt of the formula -NR+Z-, wherein R is alkyl (and in particular methyl or ethyl) or benzyl, and Z is a counteranion, including
chloride, bromide, iodide, -O-alkyl,
toluenesulfonate, methylsulfonate, sulfonate, sulfate, phosphate, or carboxylate (such as benzoate, succinate, acetate, glycolate,
propionate, maleate, malate, citrate, tartrate, ascorbate, benzoate, cinnamoate, mandeloate, benzyloate, and diphenylacetate).
Methods of synthesis of buspirone or its derivatives are disclosed in, or can be easily adapted by one of ordinary skill in organic synthesis from procedures disclosed in Wu, et al., J. Med. Chem. 15, 477 (1972), Ger. Patent No.
2,057,845, and U.S. Patent No. 3,717,634. See also J. Clin. Psychiat. 43, 1-116 (1982).
As demonstrated in Example 1, the parent
buspirone has significant immunosuppressive
activity. The potential utility of any one of the above-described buspirone derivatives to act as an immunosuppressant can be conveniently determined by synthesizing the compound and testing it in the biological assay described in Example 1.
The active compounds described herein exhibit an immunosuppressive effect when provided topically or systemically. The derivative is considered an immunosuppressant if it suppresses the ear swelling associated with an experimental contact
hypersensitivity response by at least 40% at 24 hours after specific antigen challenge.
Alternatively, the agent can be evaluated in vivo in humans by assessing the agent's ability to inhibit contact hypersensitivity responses to patch test allergens in patients hypersensitive to a given allergen, using procedures generally accepted by those of skill in the art, or by evaluation in an animal model, for example, of allograft
rejection, experimental allergic encephalomyelitis, lupus erythematosus, Freund's adjuvant arthritis and/or graft versus host disease.
For systemic administration, derivatives of buspirone which are particularly useful are those that have an immunosuppressive effect but which do not exhibit a significant neuroleptic effect, Buspirone derivatives without significant
neuroleptic effect can be identified by their ability to bind to serotonin or dopamine receptors, or by assessing their lack of ability to act as a tranquilizer or neuroleptic in mammals, for
example, by demonstrating that they are no
different than placebo, for example, in the hot plate test of Eddy, et al., J. Pharmacol. 107:385 (1953) and 110:135 (1954).
The chemically unrelated serotonin receptor antagonists, trazadone and mianserin, and the dopamine receptor antagonist, haloperidol, are not effective in suppressing contact hypersensitivity. On this basis, it appears that the mechanism of action of buspirone and buspirone derivatives in suppressing the immune response is independent of their serotonin or dopamine receptor blocking properties. Therefore, buspirone derivatives with immunosuppressive effects yet without neuroleptic effects can be provided by the method of selection disclosed generally herein.
II. Complexation or Modification of the Buspirone Nucleus to Prevent Significant Neuroleptic Effect
As discussed above, immunosuppressive compounds with a buspirone nucleus that have a neuroleptic effect can be complexed or modified to eliminate that effect, by one or more of the following processes.
A. Decreasing the Lipophilicity, or Increasing the Hydrophilicity of the Compound
Compounds with a buspirone nucleus that exhibit an immunosuppressive effect yet also exhibit a neuroleptic effect can be modified to minimize the neuroleptic effect by decreasing the lipophilicity (equivalent to increasing the hydrophilicity) of the molecule. This can be done by adding one or more charged side chain (s) onto the molecule or by altering the existing side chain to make it more polar. The hydrophilicity of buspirone derivatives will in general increase when charged substituents are added.
B. Increasing the Size of the Molecule
Another technique for reducing the central nervous system (CNS) effects of compounds that contain a buspirone nucleus is to increase the size of the molecule via a covalent linkage to a large moiety (e.g., albumin or polyethylene glycol), using standard techniques of organic synthesis or by choosing a buspirone derivative with large substituents.
C. Complexing the Buspirone Nucleus with a Cyclic Molecule
A third method for reducing the central nervous system (CNS) effects of a compound that contains a buspirone nucleus includes forming a non-covalent complex of the compound with a cyclic molecule such as a cycloamylose (e.g., a cyclodextrin such as β-cyclodextrin), which has a spatial arrangement of hydroxyl groups whereby the outer surface of the ring formed by the cycloamylose is hydrophilic and the inner surface is lipophilic.
When utilized in aqueous solution, this
structure permits molecules (or parts thereof), termed "guest molecules", which are less polar than water and which are of suitable dimensions, to be incorporated into the lipophilic inner cavity, such that the cycloamylose/guest molecule complex presents to the blood-brain barrier as a relatively large and polar compound which is unable to penetrate the barrier. Such complexes may be prepared by any method known to the art, including those described in U.S. Patent No. 4,555,504, which discloses β-cyclodextrin complexed with digoxin.
The central nervous system side effects of a buspirone derivative can be estimated using molecular modeling and/or pharmacophore analysis. The dopamine and serotonin receptors are well characterized and strategies for estimating binding of drugs to these receptors are well established.
For example, Schmidt, et al., Molecular
Pharmacology 38:511-516 (1990), describe an algorithm for estimating the binding affinity of drugs to the 5-HT receptor. Also, a composite pharmacophore analysis and chemical database screening strategy is described by Sleight, et al, Naunyn-Schmiedebergs Arch. Pharmacol. 343:109-116 (1991), and Schmidt, A.W. and Peroutka, S.J., Mol. Pharmacol. 36 (4) : 505-511 (1989). D. Administration as a Quaternary Salt
Buspirone or its above-defined derivative can be administered in the form of a pharmaceutically acceptable quaternary salt. Quaternary salts are typically less lipophilic than the corresponding unquaternized compound, and therefore have a decreased effect on the central nervous system. Nonlimiting examples of quaternary salts that can be used include salts prepared from methyl
chloride, methyl bromide, methyl iodide, methyl sulfate, methyl benzene-sulfonate, methyl p- toluenesulfonate, ethyl chloride, ethyl bromide, ethyl iodide, n-propyl chloride, n-propyl bromide, n-butyl bromide, isobutyl bromide, sec-butyl bromide, n-amyl bromide, n-hexyl chloride, benzyl chloride, benzyl bromide, and ethyl sulfate. Other nonlimiting examples of quaternary salts
specifically include the quaternary ammonium salt of the formula -NR3 +Z-, wherein R is alkyl or benzyl, and Z is a counteranion, including
chloride, bromide, iodide, -O-alkyl,
toluenesulfonate, methylsulfonate, sulfonate, phosphate, or carboxylate (such as benzoate, succinate, acetate, glycolate, succinate, maleate, malate, citrate, tartrate, ascorbate, benzoate, cinnamoate, mandeloate, benzyloate, and
diphenylacetate).
The methyl ammonium tosylate salt of buspirone has been found to be toxic to mice at elevated dosage levels (above 10 mg/kg). Therefore, this quaternary salt of buspirone or its derivatives should be administered as the lowest dosage that achieves a desired effect.
II. Therapeutic Compositions
Buspirone or its derivative can be included in a pharmaceutically acceptable carrier or diluent in an amount sufficient to deliver to a patient a therapeutic amount of compound in vivo in the absence of serious toxic effects for any of the above described disorders.
The concentration of active compound in the drug composition will depend on absorption,
inactivation, and excretion rates of the drug as well as other factors known to those of skill in the art. It is to be noted that dosage values will also vary with the severity of the condition to be alleviated. It is to be further understood that for any particular subject, specific dosage regimens should be adjusted over time according to the individual need and the professional judgment of the person administering or supervising the administration of the compositions, and that the dosage ranges set forth herein are exemplary only and are not intended to limit the scope or practice of the claimed composition. The active ingredient may be administered once, or may be divided into a number of smaller doses to be administered at varying intervals of time.
Buspirone or its derivative can be mixed with other active materials which do not impair the desired action, or with materials that supplement the desired action, such as antibiotics,
antifungals, anti-inflammatories, antivirals, or other immunosuppressive agents.
Buspirone or its derivatives can be provided in the form of pharmaceutically-acceptable salts. As used herein, the term "pharmaceutically-acceptable salts or complexes" refers to salts or complexes that retain the desired biological activity of the parent compound and exhibit minimal, if any, undesired toxicological effects. Examples of such salts are (a) acid addition salts formed with inorganic acids (for example, hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid, and the like), and salts formed with organic acids such as acetic acid, oxalic acid, tartaric acid, succinic acid, malic acid, ascorbic acid, benzoic acid, tannic acid, pamoic acid, alginic acid, polyglutamic acid,
naphthalenesulfonic acids, naphthalenedisulfonic acids, and polygalacturonic acid; (b) base addition salts formed with polyvalent metal cations such as zinc, calcium, bismuth, barium, magnesium,
aluminum, copper, cobalt, nickel, cadmium, and the like, or with an organic cation formed from N,N- dibenzylethylene-diamine or ethylenediamine; or (c) combinations of (a) and (b); e.g., a zinc tannate salt or the like.
Buspirone or its derivatives can be modified in order to enhance their usefulness as pharmaceutical compositions. For example, it is well know in the art that various modifications of the active molecule, such as alteration of charge, can affect water and lipid solubility and thus alter the potential for percutaneous absorption. The
vehicle, or carrier, can also be modified to enhance cutaneous absorption, enhance the reservoir effect, and minimize potential irritancy or
neuropharmacological effects of the composition. See, in general, Arndt, K.A., P.V. Mendenhall, "The
Pharmacology of Topical Therapy", Dermatology in General Medicine, 1987; T.B. Fitzpatrick, A.Z. Eisen, K. Wolff, I.M. Freedberg and K.F. Austen, eds., 3d ed., McGraw Hill, Inc., New York, pp.
2532-2540.
Compounds that are useful are typically those that have a therapeutic index of at least 2, and preferably 5 or 10 or greater, wherein therapeutic index is defined as EC50/IC50. A. Topical Administration
Mammals, and specifically humans, suffering from pathological cutaneous, ocular, or mucosal immune responses can be treated by topical administration to the patient of an effective amount of buspirone or its derivative or its salt, optionally in combination with a pharmaceutically acceptable carrier or diluent.
The active compound is administered topically in an effective dosage range to cause
immunosuppression of the target pathological immune response. The active compound is included in the pharmaceutically acceptable topical carrier or diluent in an amount sufficient to deliver to a patient a therapeutic amount of the buspirone derivative locally in the absence of serious toxic effects. In general, local immunosuppression can be achieved by topically administering lower doses of buspirone derivatives than would be required if the agents were administered systemically. Typical dosages for topical application for all of the above-identified conditions are those ranging from 0.001 to 100% by weight of the active compound.
Solutions or suspensions for topical application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. The pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide.
Suitable vehicles or carriers for topical application can be prepared by conventional techniques, such as lotions, suspensions,
ointments, creams, gels, tinctures, sprays, powders, pastes, slow-release transdermal patches, suppositories for application to rectal, vaginal, nasal or oral mucosa mouth washes, swish and/or spit solutions. In addition to the other materials listed above for systemic administration,
thickening agents, emollients, and stabilizers can be used to prepare topical compositions. Examples of thickening agents include petrolatum, beeswax, xanthan gum, or polyethylene, humectants such as sorbitol, emollients such as mineral oil, lanolin and its derivatives, or squalene. A number of solutions and ointments are commercially available, especially for ophthalmic applications.
Thickening agents, emollients, and stabilizers can be used to prepare topical compositions.
Examples of thickening agents include petrolatum, beeswax, xanthan gum, or polyethylene glycol, humectants such as sorbitol, emollients such as mineral oil, lanolin and its derivatives, or squalene. A number of solutions and ointments are commercially available, especially for ophthalmic and dermatologic applications.
Natural or artificial flavorings or sweeteners can be added to enhance the taste of topical preparations applied for local effect to mucosal surfaces. Inert dyes or colors can be added, particularly in the case of preparations designed for application to oral mucosal surfaces.
Buspirone or its derivative can be applied in a time release formulation via transdermal patches or by slow release polymers. The active compounds can be prepared with carriers that will protect the compound against rapid release, such as a
controlled release formulation, including implants and microencapsulated delivery systems.
Biodegradable, biocompatible polymers can be used, such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Many methods for the preparation of such formulations are patented or generally known to those skilled in the art.
B. Systemic Administration
Mammals, and specifically humans, suffering from pathogenic immune responses can also be treated by the systemic administration to the patient of an effective amount of the buspirone derivative or its salt optionally in combination with a
pharmaceutically acceptable carrier or diluent for systemic delivery.
The buspirone derivative can be administered, for example, subcutaneously, intravenously, intraperitoneally, intramuscularly, parenterally, orally, submucosally, by inhalation, or
transdermally via a slow release patch, in an effective dosage range to cause systemic
immunosuppression. Typical systemic dosages for all of the above-identified conditions are those ranging from 20 mg/kg to 0.0001 mg/kg per day as a single daily dose or divided daily doses. The effective dosage of the parent compound, buspirone, for systemic immunosuppression is believed to be higher than the effective dosage of buspirone for inducing a neuroleptic effect.
The buspirone derivative is administered
systemically for a sufficient time period to alleviate the undesired symptoms and the clinical signs associated with the condition being treated.
A preferred mode of systemic administration of the active compound is oral. Oral compositions will generally include an inert diluent or an edible carrier. They may be enclosed in gelatin capsules or compressed into tablets. For the purpose of oral therapeutic administration, the active compound can be incorporated with excipients and used in the form of tablets, troches, or capsules. Pharmaceutically compatible binding agents, and/or adjuvant materials can be included as part of the composition.
The tablets, pills, capsules, troches and the like can contain any of the following ingredients, or compounds of a similar nature: a binder such as microcrystalline cellulose, gum tragacanth or gelatin; an excipient such as starch or lactose, a disintegrating agent such as alginic acid,
Primogel, or corn starch; a lubricant such as magnesium stearate or Sterotes; a glidant such as colloidal silicon dioxide; a sweetening agent such as sucrose or saccharin; or a flavoring agent such as peppermint, methyl salicylate, or orange
flavoring.
When the dosage unit form is a capsule, it can contain, in addition to material of the above type, a liquid carrier such as a fatty oil. In addition, dosage unit forms can contain various other
materials which modify the physical form of the dosage unit, for example, coatings of sugar, shellac, or other enteric agents.
The buspirone derivative or its salts can be administered as a component of an elixir,
suspension, syrup, wafer, chewing gum or the like. A syrup may contain, in addition to the active compounds, sucrose as a sweetening agent and certain preservatives, dyes and colorings and flavors.
Solutions or suspensions used for parenteral, intradermal, or subcutaneous, application can include the following components: a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl parabens; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as ethylenediaminetetraacetic acid; buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose. pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide. The parenteral preparation can be enclosed in ampoules, disposable syringes or multiple dose vials made of glass or plastic.
If administered intravenously, preferred
carriers are bacteriostatic water, physiological saline, Cremophor EL™ (BASF, Parsippany, NJ) or phosphate buffered saline (PBS).
In one embodiment, the active compounds are prepared with carriers that will protect the compound against rapid elimination from the body on systemic delivery, such as a controlled release formulationo including implants and
microencapsulated delivery systems. Biodegradable, biocompatible polymers can be used, such as
ethylene vinyl acetate, polyanhydrides,
polyglycolic acid, collagen, polyorthoesters, and polylactic acid. Methods for preparation of such formulations will be apparent to those skilled in the art.
Liposomal suspensions (including liposomes targeted to infected cells with monoclonal
antibodies to specific antigens) can also be used as pharmaceutically acceptable carriers. These may be prepared according to methods known to those skilled in the art, for example, as described in
U.S. Patent No. 4,522,811 (which is incorporated herein by reference in its entirety) . For example, liposome formulations may be prepared by dissolving appropriate lipid(s) (such as stearoyl phosphatidyl ethanolamine, stearoyl phosphatidyl choline, arachadoyl phosphatidyl choline, and cholesterol) in an organic solvent that is then evaporated, leaving behind a thin film of dried lipid on the surface of the container. An aqueous solution of the buspirone derivative is then introduced into the container. The container is then swirled by hand to free lipid material from the sides of the container and to disperse lipid aggregates, thereby forming the liposomal suspension.
III. Immunosuppressant Activity of Buspirone
Derivatives Buspirone and buspirone derivatives are capable of acting systemically or topically to suppress the immune response in humans and other mammals. As such, the compounds, or therapeutic compositions thereof, are useful for the treatment of a myriad of immunological disorders or other pathological conditions associated with an immune response.
Examples of such disorders include cutaneous contact hypersensitivity, host rejection of foreign organ or tissue transplants; graft-vs-host disease in which donor immunological cells present in the graft attack host tissues in the recipient of the graft; diseases with proven or possible autoimmune components, such as rheumatoid arthritis and juvenile rheumatoid arthritis, aphthous ulcer, lichen planus, psoriatic arthritis, psoriasis, excema, conjunctivitis, Sjogren's Syndrome, including keratoconjunctivitis sicca secondary to Sjogren's Syndrome, iritis, alopecia areata, cutaneous lupus erythematosus, scleroderma, vaginitis, proctitis, drug eruptions, leprosy reversal reactions, erythema nodosum leprosum, autoimmune uveitis, multiple sclerosis, allergic encephalomyelitis, systemic lupus erythematosis, acute necrotizing hemorrhagic encephalopathy, idiopathic bilateral progressive sensorineural hearing loss, aplastic anemia, pure red cell anemia, idiopathic thrombocytopenia,
polychondritis, scleroderma, Wegener's
granulomatosis, chronic active hepatitis,
myasthenia gravis, Stevens-Johnson syndrome, idiopathic sprue, Crohn's disease, ulcerative colitis, Graves ophthalmopathy, sarcoidosis, primary biliary cirrhosis, primary juvenile diabetes, uveitis posterior, and interstitial lung fibrosis; allergic asthma; allergic responses due to arthropod bite reactions, asthma, allergic asthma, and inappropriate allergic responses to other environmental stimuli such as atopic
dermatitis and hypersensitivity to pollen, insect stings and certain foods. These compounds can also be useful in reducing the infiltration of skin by malignant leukocytes in diseases such as mycosis fungoides.
Buspirone and its derivatives can also be used to increase tear production in a patient suffering from deficient tears in the eye due to an
autoimmune dysfunction of the lacrimal glands, such as immune mediated keratoconjunctivitis (KCS, or dry eye) . Canine KCS is a common, chronic
progressive, and potentially blinding disease. A continuum of corneal and conjunctival lesions ensues from the dry eye state. Buspirone or its active derivatives can be provided as an ophthalmic drop or ophthalmic ointment to humans or other mammals, including dogs and cats, in an effective amount in a suitable vehicle. This topical ophthalmic treatment can also serve to correct corneal and conjunctival disorders exacerbated by tear deficiency and KCS, such as corneal scarring, corneal ulceration, filamentary keratitis,
mucopurulent discharge, and vascularization of the cornea. Buspirone and its derivatives can also be used to decrease immune responses which contribute to granulation and neovascularation in the cornea.
The ability of buspirone to influence the tissue swelling associated with contact hypersensitivity reactions in mice was evaluated as described in detail in Example 1. Buspirone HCl was used for the procedure in Example 1 as a model of an active immunosuppressant. Buspirone derivatives can be measured against this model, and are considered active if they suppress the swelling response by at least 40% 24 hours after specific antigen
challenge.
When applied topically, preparations of
buspirone significantly suppressed the tissue swelling associated with the elicitation phase of contact hypersensitivity to oxazolone. However, mice treated topically with buspirone HCl, unlike those treated systemically, exhibited no drowsiness or other evidence of central nervous system
effects.
Buspirone expresses both serotonin and dopamine receptor antagonist activity. However, unlike buspirone, it was discovered that the chemically unrelated serotonin antagonists, trazadone and mianserin, and the dopamine receptor antagonist, haloperidol, were not effective in suppressing contact hypersensitivity. On the basis of this, it appears that the mechanism of action of buspirone on the immune response is independent of its serotonin or dopamine receptor blocking properties, and therefore, buspirone derivatives with
immunosuppressive effect yet without neuroleptic effect can be provided by the method of selection disclosed generally herein. Example 1: Inhibition of Induced Contact
Hypersensitivity.
Six-to-8-week-old female C57BL/6J or BALB/c mice were obtained from the Jackson Laboratory, Bar Harbor, Maine or from Charles River Laboratories,
Kingston Facility, Stoneridge, NY, respectively.
Buspirone HCl, mianserin, trazadone, haloperidol and oxazolone were purchased from the Sigma
Chemical Co. (St. Louis, MO).
Oxazolone-Induced Contact Hypersensitivity -
Sensitization and challenge for contact
hypersensitivity were performed as follows. The abdomens of the mice were shaved with electric clippers, 50 μl of a 4% (w/w) solution of oxazolone in 4 : 1 (v:v) acetone: olive oil were applied to the shaved abdomen, and 5 μl of the same solution were applied to each hind footpad. Five to eight days later, the mice were challenged for contact
hypersensitivity by applying 10 μl of a 0.5% (w:w) solution of oxazolone in 4 : 1 (v:v) acetone: olive oil to both the inner and outer surface of the right ear of each mouse (in the case of mice treated systemically with buspirone HCl) or to both ears (in the case of mice treated topically with buspirone HCl) - except in the case where
sensitization phase suppression is studied, as in Figures 9 and 10.
Systemic Buspirone HCl Treatment - One hour after the application of oxazolone for elicitation of contact hypersensitivity, mice were treated subcutaneously with buspirone HCl 500 or 50 mg/kg body weight) in 0.1 mL of carrier (Cremophor EL, BASF, Parsippany, NJ), or with 0.1 mL of carrier alone. In a separate experiment, mice were treated in a similar fashion with 50 mg/kg body weight of trazadone, mianserin, haloperidol, or buspirone HCl in 1 mL olive oil or with olive oil alone. Topical Buspirone HCl Treatment For these experiments, both ears of each mouse were
challenged for elicitation of contact
hypersensitivity by the application of oxazolone (as appropriate) to both surfaces of both ears.
Two hours before, or twenty-four hours after application of hapten, the right ears of some mice were treated with buspirone HCl in vehicle, applied epicutaneously to both surfaces. The right ears of control mice were similarly treated, but with vehicle alone. In the case of experiments designed to assess topical effects on the sensitization phase, only the right ear is challenged. (See Figures 9 and 10)
Evaluation of Ear Swelling Response -
Immediately before and 24 or 48 hours after application of oxazolone, ear thicknesses were determined with an engineer's micrometer. The increment (delta) in ear thickness (ear swelling) was calculated as the 24- or 48-hour value minus the baseline (pre-challenge) value and expressed in units of 10-4 inches. Mice were killed by cervical dislocation after the measurement of 24-hour ear thickness was obtained, and the ears were processed for histologic examination.
Quantification of Leukocyte Infiltration - Both ears of each mouse were fixed in 4.0% buffered formalin and then processed routinely and embedded in paraffin for preparation of 6-7 μm-thick
hematoxylin and eosin-stained sections. All of the sections were coded and examined with an ocular grid at 400x under light microscopy by an observer unaware of the identity of the individual slides. The number of leukocytes/mm2 of dermis was
calculated by counting all of the leukocyte cells in an area of at least 0.14 mm2 of dermis. Statistical Analysis - Differences between groups were assessed by the 2-tailed Student's t test (paired for comparisons of left and right ears in the same mice, unpaired for comparisons between different groups of mice).
Effect of Topical Buspirone HCl on Expression of Contact Hypersensitivity - Figures 1 and 2 illustrate the effect of topical application of 100 mg/mL of buspirone HCl (Group B) or carrier alone (Group A) on expression of contact
hypersensitivity. As indicated, topical
administration of buspirone HCl at 100 mg/mL significantly decreased ear swelling (Figure 1) and aggregation of leukocytes (Figure 2).
Importantly, while topical application of buspirone
HCl was extremely effective in diminishing both the tissue swelling and the leukocyte infiltration associated with contact hypersensitivity reactions, these effects were observed in the absence of detectable alterations in the behavior of the mice.
The mice treated topically with buspirone HCl appeared active and retained apparently normal interest in food and water.
Effect of Systemic Buspirone Versus Other
Serotonin or Dopamine Receptor Antagonists - In these experiments, systemic buspirone was compared to the serotonin receptor antagonists, trazadone or mianserin, and to the dopamine receptor antagonist, haloperidol, for their ability to inhibit cutaneous contact hypersensitivity. At a dose of 50 mg/kg, only buspirone HCl significantly reduced cutaneous contact hypersensitivity (Figures 3 and 4).
Effect of Topically Administered Buspirone on Tissue Swelling Associated with Oxazolone-Induced Contact Hypersensitivity Reactions/Effect of Time of Administration of Topical Buspirone. Oxazolone was applied to both ears of all mice at different times either pre- or post-buspirone treatment, and the change in ear thickness was measured at a specified interval thereafter. a. Two hours before oxazolone challenge, 100 mg/mL buspirone HCl in Vehicle-N was applied to both surfaces of the right ears of some mice, whereas vehicle alone was applied to both surfaces of the ears of the control (0% buspirone) animals. The ears were measured 24 hours after oxazolone challenge (Figure 5a). Local pre-challenge treatment of the right ear with buspirone HCl significantly suppressed tissue swelling in the treated ear (**p<0.01 vs
contralateral oxazolone treated ears or vs right ears of vehicle treated group). Treatment of the right ear with 100 mg/mL buspirone HCl had no significant effect on the magnitude of swelling in the contralateral oxazolone treated ear. b. In a separate experiment, twenty-four hours after oxazolone challenge, 100 mg/mL buspirone HCl in Vehicle-N was applied to both surfaces of the right ears of some mice, whereas vehicle alone was applied to both surfaces of the ears of control (0% buspirone) mice. The change in ear thickness was determined 24 hours after treatment with buspirone, i.e. at 48 hours after challenge with oxazolone
(Figure 5b). Treatment with buspirone HCl
significantly diminished contact hypersensitivity reactions in the right ears of the treated animals (*=p<0.01 when compared to the right ears in the control mice, and p<0.05 when compared to the contralateral ears of the same mice). The
reactions in the left ears of the mice treated on the right ears with buspirone HCl were not reduced wnen compared to reactions in the left ears of the vehicle-treated mice. When the ears of the same mice shown in Figures 5a and 5b were examined histologically (Figures 6a and 6b), buspirone HCl treatment was shown to diminish significantly the leukocyte infiltration associated with the contact hypersensitivity response (**=p<0.01 when compared to the right ears in the vehicle-tested mice) .
Effect of Systemic Treatment with Buspirone on
Expression of Contact Hypersensitivity - The subcutaneous administration of buspirone HCl at dosages of 500 or 50 mg/kg, 1 hour after challenge markedly diminished the tissue swelling which developed in association with the contact
hypersensitivity response (Figure 7). The
leukocyte infiltration associated with the response in mice treated with 500 or 50 mg/kg buspirone HCl was also diminished compared to responses in mice not treated with the drug (Figure 8). However, at these dosages, buspirone HCl also produced other remarkable systemic effects. The mice rapidly became lethargic after administration of the drug, and, by 23 hours after buspirone HCl injection, the mice exhibited profound depression of central nervous system function (these effects were more pronounced in the high dosage group) . They
appeared to be in a deep sleep, neither ate nor drank, and responded weakly or not at all to touch. They did, however, exhibit responsiveness to pinch, in both dosages.
Example 2 : Comparison of Immunosuppressant versus
Anti-inflammatory activity.
Mice were sensitized to oxazolone as described in Example 1. Three days later, slow release indomethacin pellets (0.05 mg, 3 week release) were implanted subcutaneously under light ether
anesthesia. The dose of indomethacin delivered by these pellets has been previously shown to
completely block prostaglandin synthesis in mice, by Jun, D.D., et al., J. Invest. Dermatol. 90:311 (1988). Three days later, mice were challenged for contact hypersensitivity as in Example 1. When the hypersensitivity response was assessed 24 hours later, indomethacin was shown to have no
significant effect on the response. These figures
(11 and 12) show that a classic anti-inflammatory agent, indomethacin, does not appear to suppress the edema associated with the immunologically specific oxazolone induced contact hypersensitivity response and compared to buspirone HCl, only weakly suppresses the leukocyte infiltration associated with the response.
Example 3: Evaluation of Serotonin Receptor
Binding Activity or Dopamine Receptor Binding Activity of Buspirone
Derivatives.
Buspirone derivatives which lack serotonin receptor binding or dopamine receptor binding activity can be identified as follows. A
radiolabeled ligand known to bind serotonin and/or dopamine receptors can be bound to an appropriate substrate expressing one or both of these
receptors. For example, radiolabeled quipazine which is available commercially can be used as the ligand. The buspirone derivative to be tested is then incubated with the radiolabeled quipazine ligand combination. Displacement of radiolabeled ligand is positive evidence that the buspirone derivative being tested can bind serotonin and/or dopamine receptors. The amount of radiolabeled ligand which is displaced is determined by an appropriate standard curve which can also provide information concerning binding affinities. The displaced radiolabeled ligand can be quantitated using a standard scintillation counter.
A detailed description of how to perform the binding studies using 3H-quipazine and the example follows: Binding studies using 3H-quipazine are described in detail by Milburn, CM. and Peroutka, S.J., J. Neurochem. 52:1787-1792 (1989). Briefly, rat cortices are homogenized in 20 volumes of 50 mM Tris HCl buffer pH 7.7 at 25 °C and centrifuged at
49,000 x g for 10 min. The pellet is resuspended in fresh buffer and incubated at 37°C for 10 min. After the final centrifugation, the pellet is resuspended in 80 volumes of Krebs-HEPES buffer (25 mM HEPES, 118 mM NaCl, 5 mM KCl, 2.5 mM CaCl2, and
1.2 mM MgCl2 pH adjusted to 7.4). Tissue (10 mg of original wet weight) is added to assay tubes containing 0.8 nM [3H] quipazine and displacing drug or buffer in a final volume of 1 mL. Non-specific binding is defined using 1 micromole zacopride.
After a 30 min incubation at room temperature, the tissue is rapidly filtered under vacuum through No. 32 glass fiber filters and rinsed twice with 5 mL of 50 mM Tris-HCl buffer pH 7.7. Radioactivity is quantified by liquid scintillation counting. All experiments are performed three to six times, each in triplicate. This same approach can be used with other radiolabeled ligands such as zacopride, granisetron, haloperidol, mianserin, ketanserin, 5- HT, dopamine, droperidol, or ritanserin.
Buspirone derivatives which have binding
affinities for dopamine and/or serotonin receptors of one/tenth or less than native buspirone are considered to be potentially useful as systemic immunosuppressants if they are at least 50% as active as native buspirone on a weight basis in suppressing immunologically specific responses such as contact hypersensitivity.
Modifications and variations of the present invention will be obvious to those skilled in the art from the foregoing detailed description of the invention. Such modifications and variations are intended to come within the scope of the appended claims.

Claims

We claim:
1. A method for the treatment of a cutaneous, ocular, or mucosal pathology associated with an immune response in a human or other mammal that includes topical application of an effective amount of a compound selected from the group consisting of buspirone and a buspirone derivative of the formula
Figure imgf000042_0001
wherein:
R1 = H; halo (chloro, bromo, fluoro, or iodo);
alkyl, specifically including CH3-, cyclohexyl, (CH3)2CH-, CH3(CH2)3-,
(CH3)2CHCH2-, CH3CH2CH(CH3)-, (CH3)3C-, and
-CH3(CH2)p; Y-CH2(CH2)n-; oxyalkyl; C6H5-, aryl, (2, 3, or 4) -(OCH3) C6H4- and (2, 3, or
4)-(CH3)C6H4-; 2-X-C6H4-, 3-X-C6H4-, or 4-X-
C6H4-; oxyaryl; or alkaryl;
R2 = H, C6H5CH(CH2CH3)CH2-, C6H5CH (CH3) (CH2)2-,
C6H;CH2CH (CH3) CH2-, C6H5CH2CH2CH (CH3)-,
C6H<CH(CH3) (CH2)3-,
(2, 3, or 4)-(alkyl)-C6H4CH(CH3) (CH2)3-,
(2, 3, or 4)-(alkyloxy)- C6H4CH(CH3) (CH2)3,
(2, 3, or 4)-X-C6H4-alkyl,
(2, 3, or 4)-X-C6H4CH(CH2CH3)CH2-,
(2, 3, or 4)-X-C6H4CH(CH3) (CH2)-
4-X-C6H4CH(CH3) (CH2)2-, and
4-X-C6H4-CH(CH3) (CH2)3-; C6H5CH(OCH3) (CH2)2-,
C6H5CH--CH-CH2-, C6H5CO(CH2)3-, C6H5CO (CH2)4-,
Figure imgf000042_0002
(2, 3, or 4)-(alkyl)-C6H4CO(CH2)3-,
(2, 3, or 4)-(alkyl-oxy)-C6H4CO(CH2)3-, (2, 3, or 4)-X-C6H4CO (CH2)n-,
2-thienyl-CO-(CH2)3-,
-alkyl-piperazinyl-aryl;
-alkyl-C3-8cycloalkyl-aryl;
-alkyl-piperazinyl-heterocycle;
-alkyl-C3-8cycloalkyl-heterocycle;
-alkyl-C3-8cycloalkyl-Ar1;
-alkyl-piperazinyl-Ar1;
-alkenyl-piperazinyl-aryl;
-alkenyl-C3-8cycloalkyl-aryl;
-alkyl-aryl-heterocycle;
-alkyl-heterocycle-aryl;
-alkenyl-C3-8cycloalkyl-Ar1;
-alkenyl-piperazinyl-heterocycle; -alkenyl-C3-8cycloalkyl-heterocycle;
-alkenyl-piperazinyl-Ar1;
Ar1-CH(CH2)n-,
Ar
Figure imgf000043_0001
1
(2, 3, or 4)-X-C6H4C(CH3)CH(CH2)2-, where the conformation about the double bond is cis or trans,
(2, 3, or 4)-X-C6H4C(CH3)CHCH2-, where the conformation about the double bond is cis or trans,
(2, 3, or 4 ) -X-C6H4COCH=CHCH2-,
Y-CH2(CH2)n-, Ar1-(CH2)n-, C1 to C20 alkyl,
X-(CH2)nCO-, or X-(CH2)n-;
R3 = =O, =NH, =S, chloro, bromo, iodo, fluoro, alkyl, or aryl;
n = 1 to 6;
p = 1 to 20;
X = is independently F, Cl, Br, I, OCH3, SO3-,
NH2, H, -OH, -COOH, -COOR, -SO3H, -CN,
-NHSO3H, -N02, or -SO2NH2;
Y = H, F, Cl, Br, I, -SO3, -PO4 =, -OH, -SH,
-SCH3,
-CH3SO2-, -NH2, or -CO2-; and Ar1 = independently, aryl, (2, 3, or 4-X-C6H4-),
(2, 3, or 4)-(CH2X)C6H4-, (2, 3, or 4)-
(CX3)C6H4-,
(2, 3, or 4)-(CHX2)C6H4-, 2-thienyl, or
(2, 3, or 4)-X-C6H4CH2- or its pharmaceutically acceptable salt, including a quaternary salt, optionally in a pharmaceutically-acceptable diluent or carrier.
2. The method of claim 1, wherein the alkyl group is selected from the group consisting of CH3-, cyclohexyl, (CH3)2CH-, CH3(CH2)3-, (CH3)2CHCH2-,
CH3CH2CH(CH3)-, -CH3(CH2)p and (CH3)3C-.
3. The method of claim 1, wherein the aryl group is selected from the group consisting of C6H5-, (2, 3, or 4)-(OCH3)C6H4- and (2, 3, or 4)-
(CH3)C6H4-; 2-X-C6H4-, 3-X-C6H4-, or 4-X-C6H4-.
4. The method of claim 1, wherein
(2, 3, or 4) -X-C6H4-alkyl- is selected from the group consisting of (2, 3, or 4) -X-C6H4CH(CH2CH3) CH2-, (2, 3, or 4)-X-C6H4CH(CH3) (CH2)-,
(2, 3, or 4)-X-C6H4CH(CH3) (CH2)2-, and
(2, 3, or 4)-X-C6H4-CH(CH3) (CH2)3-.
5. The method of claim 1, wherein the
quaternary ammonium salt is of the formula -NR3 +Z-, wherein R is alkyl or benzyl, and Z is a
counteranion selected from the group consisting of chloride, bromide, iodide, -O-alkyl,
toluenesulfonate, methylsulfonate, sulfonate, phosphate, benzoate, succinate, acetate, glycolate, succinate, maleate, malate, citrate, tartrate, ascorbate, benzoate, cinnamoate, mandeloate, benzyloate, and diphenylacetate.
6. The method of claim 1 wherein the mammal is a human.
7. The method of claim 1, wherein the
pathology associated with an immune response is selected from the group consisting of contact dermatitis, atopic dermatitis, Sjogren's Syndrome, including keratoconjunctivitis sicca secondary to Sjogren's Syndrome, eczematous dermatitis, drug eruptions, lichen planus, psoriasis, alopecia areata, cutaneous lupus erythematosus, scleroderma, asthma, allergic asthma, ulcerative colitis,
Crohn's disease, ulcerative colitis allergic reactions secondary to arthropod bite reactions, aphthous ulcers, conjunctivitis, iritis,
keratoconjunctivitis, vaginitis, and proctitis.
8. The method of claim 1, wherein the carrier is a mouthwash.
9. The method of claim 1, wherein the carrier is a swish and spit solution.
10. The method of claim 1 wherein the compound in combination with an ophthalmic carrier is topically applied to the eye.
11. The method of claim 1, wherein the compound is applied cutaneously.
12. The method of claim 1, wherein the compound is applied to mucosal membranes.
13. The method of claim 1, wherein the daily dose of compound is between 0.01 and 60 grams.
14. The method of claim 1, wherein the compound is applied in a concentration between 0.01% and
10%.
15. The method of claim 1 wherein the compound is administered in a time release formulation.
16. The method of claim 1, wherein the compound is administered via a retention enema.
17. The method of claim 1, wherein the compound is administered in combination with another
compound or compounds selected from the group consisting of antivirals, antifungals, antibiotics, anti-inflammatories, and other immunosuppressants and bronchodilators or other therapeutic agents for asthma.
18. A method for treating a mammal in need of immunosuppression, comprising systemically
administering to the mammal an effective amount of buspirone or a buspirone derivative without significant neuroleptic effect of the formula:
wherein:
Figure imgf000046_0001
R1 = H; halo (chloro, bromo, fluoro, or iodo);
alkyl, specifically including CH3-, cyclohexyl, CH3)2CH-, CH3(CH2)3-, (CH3)2CHCH2-,
CH3CH2CH(CH3)-, (CH3)3C-, and -CH3(CH2)p;
Y-CH2(CH2)n-; oxyalkyl; C6H5-, aryl,
(2, 3, or 4)-(OCH3)C6H4- and
(2, 3, or 4)-(CH3)C6H4-;
2-X-C6H4-, 3-X-C6H4-, or 4-X-C6H4-; oxyaryl; or alkaryl;
R, = H , C6H5CH (CH2CH3) CH2-, C6H5CH (CH3) (CH2)2-,
C6H5CH2CH (CH3) CH2-, C6H5CH2CH2CH (CH3)-,
C6H5CH(CH3) (CH2)3-,
(2, 3, or 4)-(alkyl)-C6H4CH(CH3) (CH2)3-,
(2, 3, or 4)-(alkyloxy)- C6H4CH(CH3) (CH2)3, (2, 3, or 4)-X-C6H4-alkyl,
(2, 3, or 4)-X-C6H4CH(CH2CH3)CH2-,
(2, 3, or 4)-X-C6H4CH(CH3) (CH2)-
4-X-C6H4CH(CH3) (CH2)2-, and
4-X-C6H4-CH(CH3) (CH2)3-; C6H5CH(OCH3) (CH2)2-,
C6H5CH-CH-CH2-, C6H5CO(CH2)3-, C6H5CO(CH2)4 -,
CH
Figure imgf000046_0002
2/
(2, 3, or 4)-(alkyl)-C6H4CO(CH2)3-,
(2, 3, or 4)-(alkyl-oxy)-C6H4CO(CH2)3-,
(2, 3, or 4)-X-C6H4CO(CH2)n-,
2-thienyl-CO-(CH2)3-, -alkyl-piperazinyl-aryl;
-alkyl-C3-8cycloalkyl-aryl ;
-alkyl-piperazinyl-heterocycle;
-alkyl-C3-8cycloalkyl-heterocycle;
-alkyl-C3-8cycloalkyl-Ar1;
-alkyl-piperazinyl-Ar1;
-alkenyl-piperazinyl-aryl;
-alkenyl-C3-8cycloalkyl-aryl ;
-alkyl-aryl-heterocycle;
-alkyl-heterocycle-aryl;
-alkenyl-C3-8cycloalkyl-Ar1 ;
-alkenyl-piperazinyl-heterocycle;
-alkenyl-C3-8cycloalkyl-heterocycle ;
-alkenyl-piperazinyl-Ar1;
Ar1-CH(CH2)n-,
Ar
Figure imgf000047_0001
f
(2, 3, or 4)-X-C6H4C(CH3)CH(CH2)2-, where the conformation about the double bond is cis or trans,
(2, 3, or 4)-X-C6H4C(CH3)CHCH2-, where the conformation about the double bond is cis or trans,
(2, 3, or 4 ) -X-C6H4COCH=CHCH2-,
Y-CH2(CH2)n-, Ar1-(CH2)n-, C, to C20 alkyl, X- (CH2)nCO-, or X-(CH2)n-;
R3 = =O, =NH, =S, chlorr, bromo, iodo, fluoro, alkyl, or aryl;
n = 1 to 6;
p = 1 to 20;
X = is independently F, Cl, Br, I, OCH3, SO3-,
NH2, H,
-OH, -COOH, -COOR, -SO3H, -CN, -NHSO3H, -NO2, or -SO2NH2;
Y = H, F, Cl, Br, I, -SO3, -PO4 =, -OH, -SH,
-SCH3, -CH3SO2-, -NH2, or -CO2-; and
Ar1 = independently, aryl, (2, 3, or 4-X-C6H4-),
(2, 3, or 4)-(CH2X)C6H4-, ( 2 , 3 , or 4 ) - ( CX3 ) C6H4- ,
(2, 3, or 4)-(CHX2)C6H4-, 2-thienyl, or (2, 3, or 4) -X-C6H4CH2-or its pharmaceutically acceptable salt, including a quaternary salt, in a pharmaceutically-acceptable diluent or carrier.
19. The method of claim 18, wherein the alkyl group is selected from the group consisting of CH3-, cyclohexyl, (CH3)2CH-, CH3(CH2)3-, (CH3) 2CHCH2-,
CH3CH2CH(CH3)-, -CH3(CH2)p and (CH3)3C-.
20. The method of claim 18, wherein the aryl group is selected from the group consisting of C6H5-, (2, 3, or 4)-(OCH3)C6H4- and (2, 3, or 4)- (CH3)C6H4-; 2-X-C6H4-, 3-X-C6H4-, or 4-X-C6H4-.
21. The method of claim 18, wherein
(2, 3, or 4) -X-C6H4-alkyl- is selected from the group consisting of (2, 3, or 4) -X-C6H4CH(CH2CH3) CH2-, (2, 3, or 4)-X-C6H4CH(CH3) (CH2)-,
(2, 3, or 4)-X-C6H4CH(CH3) (CH2)2-, and
(2, 3, or 4)-X-C6H4-CH(CH3) (CH2)3-.
22. The method of claim 18, wherein the
quaternary ammonium salt is of the formula -NR3 +Z-, wherein R is alkyl or benzyl, and Z is a
counteranion selected from the group consisting of chloride, bromide, iodide, -O-alkyl,
toluenesulfonate, methylsulfonate, sulfonate, phosphate, benzoate, succinate, acetate, glycolate, succinate, maleate, malate, citrate, tartrate, ascorbate, benzoate, cinnamoate, mandeloate, benzyloate, and diphenylacetate.
23. The method of claim 18 wherein the mammal is a human.
24. The method of claim 18, wherein the immune response is a disorder selected from the group consisting of autoimmune diseases, diseases of known or unknown etiology having an immunological component, and allergies.
25. The method of claim 24, wherein the autoimmune disease is selected from a group
consisting of rheumatoid arthritis, juvenile rheumatoid arthritis, psoriatic arthritis,
psoriasis, leprosy reversal reactions, erythema nodosum leprosum, autoimmune uveitis, multiple sclerosis, allergic encephalomyelitis, systemic lupus erythematosis, acute necrotizing hemorrhagic encephalopathy, idiopathic bilateral progressive sensorineural hearing loss, aplastic anemia, pure red cell anemia, idiopathic thrombocytopenia, polychondritis, scleroderma, Wegener's
granulomatosis, chronic active hepatitis,
myasthenia gravis, atopic dermatitis,
Stevens-Johnson syndrome, idiopathic sprue, Crohn's disease, ulcerative colitis. Graves ophthalmopathy, sarcoidosis, primary biliary cirrhosis, primary juvenile diabetes, uveitis posterior, and
interstitial lung fibrosis.
26. The method of claim 18, wherein buspirone or the buspirone derivative is administered by intravenous injection.
27. The method of claim 26, wherein buspirone or the buspirone derivative is administered orally.
28. The method of claim 18, wherein buspirone or the buspirone derivative, or its
pharmaceutically acceptable salt, other than a quaternary salt, is administered via transdermal patch.
29. The method of claim 18, wherein the dosage is between 2.5 mg/kg and 0.001 mg/kg of body weight per day as a single daily dose or divided daily doses.
30. The method of claim 18, wherein buspirone or the buspirone derivative is administered in a time release formulation.
31. The method of claim 18, wherein buspirone or its derivative is administered subcutaneously.
32. The method of claim 18, wherein buspirone or its derivative is administered in combination with a compound or compounds selected from the group consisting of antivirals, antifungals, antibiotics, anti-inflammatories, and other immunosuppressants.
33. A pharmaceutical composition for systemic administration comprising a buspirone derivative without significant neuroleptic effect of the formula:
wherein:
Figure imgf000050_0001
R1 = H; halo (chloro, bromo, fluoro, or iodo);
alkyl, specifically including CH3-, cyclohexyl, (CH3)2CH-, CH3(CH2)3-,
(CH3)2CHCH2-, CH3CH2CH(CH3)-, (CH3)3C-, and -CH3(CH2)p; Y-CH2(CH2)n-; oxyalkyl; C6H5-, aryl, (2, 3, or 4) - (OCH3) C6H4- and (2, 3, or 4)-(CH3)C6H4-;
2-X-C6H4-, 3-X-C6H4-, or 4-X-C6H4-; oxyaryl; or alkaryl;
R2 = H, C6H5CH(CH2CH3)CH2-, C6H5CH(CH3) (CH2)2-,
C6H5CH2CH (CH3) CH2-, C6H5CH2CH2CH (CH3)-, C6H5CH(CH3) (CH2)3-,
(2, 3, or 4)-(alkyl)-C6H4CH(CH3) (CH2)3-, (2, 3, or 4)-(alkyloxy)- C6H4CH(CH3) (CH2)3, (2, 3, or 4)-X-C6H4-alkyl,
(2, 3, or 4)-X-C6H4CH(CH2CH3)CH2-,
(2, 3, or 4)-X-C6H4CH(CH3) (CH2)-
4-X-C6H4CH(CH3) (CH2)2-, and
4-X-C6H4-CH(CH3) (CH2)3-; C6H5CH(OCH3) (CH2)2-, C6H5CH- CH-CH2-, C6H5CO (CH2)3-, C6H5CO(CH2)4-,
Figure imgf000051_0002
(2, 3, or 4)-(alkyl)-C6H4CO(CH2)3-,
(2, 3, or 4)-(alkyl-oxy)-C6H4CO(CH2),-, (2, 3, or 4)-X-C6H4CO(CH2)n-,
2-thienyl-CO-(CH2)3-,
-alkyl-piperazinyl-aryl;
-alkyl-C3-8cycloalkyl-aryl;
-alkyl-piperazinyl-heterocycle;
-alkyl-C3-8cycloalkyl-heterocycle;
-alkyl-C3-8cycloalkyl-Ar1;
-alkyl-piperazinyl-Ar1;
-alkenyl-piperazinyl-aryl;
-alkenyl-C3-8cycloalkyl-aryl;
-alkyl-aryl-heterocycle;
-alkyl-heterocycle-aryl;
-alkenyl-C3-8cycloalkyl-Ar1;
-alkenyl-piperazinyl-heterocycle;
-alkenyl-C3-8cycloalkyl-heterocycle; -alkenyl-piperazinyl-Ar1;
Ar1-CH(CH2)n-,
Ar1
Figure imgf000051_0001
(2, 3, or 4)-X-C6H4C(CH3)CH(CH2)2-, where the conformation about the double bond is cis or trans,
(2, 3, or 4)-X-C6H4C(CH3)CHCH2-, where the conformation about the double bond is cis or trans,
(2, 3, or 4)-X-C6H4COCH=CHCH2-,
Y-CH2(CH2)n-, Ar1-(CH2)n-, C1 to C20 alkyl,
X-(CH2)nCO-, or X-(CH2)n-;
R3 = =O, =NH, =S, chloro, bromo, iodo, fluoro, alkyl, or aryl;
n = 1 to 6;
p = 1 to 20;
X = is independently F, Cl, Br, I, OCH3, SO3-,
NH2, H, -OH, -COOH, -COOR, -SO3H, -CN, -NHSO3H, -NO2, or -SO2NH2;
Y = H, F, Cl, Br, I, -SO3, -PO4 =, -OH, -SH,
-SCH3, -CH3SO2-, -NH2, or -CO2-; and
Ar1 = independently, aryl, (2, 3, or 4-X-C6H4-),
(2, 3, or 4)-(CH2X)C6H4-, (2, 3, or 4)-(CX3)C6H4-,
(2, 3, or 4)-(CHX2)C6H4-, 2-thienyl, or (2, 3, or 4 ) -X-C6H4CH2-or its pharmaceutically acceptable salt, including a quaternary salt, in a pharmaceutically-acceptable diluent or carrier for systemic delivery.
34. The composition of claim 33, wherein the alkyl group is selected from the group consisting of CH3-, cyclohexyl, (CH3)2CH-, CH3(CH2)3-,
(CH3)2CHCH2-, CH3CH2CH(CH3)-, -CH3(CH2)p and (CH3)3C-.
35. The composition of claim 33, wherein the aryl group is selected from the group consisting of C6H5-, (2, 3, or 4)-(OCH3)C6H4- and (2, 3, or 4)- (CH3)C6H4-; 2-X-C6H4-, 3-X-C6H4-, or 4-X-C6H4-.
36. The composition of claim 33, wherein
(2, 3, or 4) -X-C6H4-alkyl- is selected from the group consisting of (2, 3, or 4) -X-C6H4CH(CH2CH3) CH2-, (2, 3, or 4)-X-C6H4CH(CH3) (CH2)-,
(2, 3, or 4)-X-C6H4CH(CH3) (CH2)2-, and
(2, 3, or 4)-X-C6H4-CH(CH3) (CH2)3-.
37. The composition of claim 33, wherein the quaternary ammonium salt is of the formula -NR3 +Z-, wherein R is alkyl or benzyl, and Z is a
counteranion selected from the group consisting of chloride, bromide, iodide, -O-alkyl,
toluenesulfonate, methylsulfonate, sulfonate, phosphate, benzoate, succinate, acetate, glycolate, succinate, maleate, malate, citrate, tartrate, ascorbate, benzoate, cinnamoate, mandeloate, benzyloate, and diphenylacetate.
38. The composition of claim 33 in a topical carrier in an amount by weight of between 0.001 and 100% buspirone derivative.
39. The composition of claim 33, wherein the buspirone derivative comprises buspirone complexed with a cycloamylose.
40. The composition of claim 33, wherein the buspirone derivative is in a time release
formulation.
41. The composition of claim 33, wherein the buspirone derivative is administered in combination with a compound or compounds selected from the group consisting of antivirals, antifungals, antibiotics, anti-inflammatories, and other
immunosuppressants.
42. A pharmaceutical composition for topical delivery comprising an effective amount of
buspirone or a buspirone derivative of the formula:
Figure imgf000053_0001
wherein:
R1 = H; halo (chloro, bromo, fluoro, or iodo) ;
alkyl, specifically including CH3-; cyclohexyl, (CH3)2CH-, CH3(CH2)3-,
(CH3)2CHCH2-, CH3CH2CH(CH3)-, (CH3)3C-, and -CH3(CH2)p; Y-CH2(CH2)n-; oxyalkyl; C6H5-, aryl, (2, 3, or 4) - (OCH3) C6H4- and (2, 3, or
4)-(CH3)C6H4-; 2-X-C6H4-, 3-X-C6H4-, or 4-X-C6H4-; oxyaryl; or alkaryl;
R2 = H, C6H5CH(CH2CH3)CH2-, C6H5CH(CH3) (CH2) 2-,
C6H5CH2CH (CH3) CH2-, C6H5CH2CH2CH (CH3)-, C6H5CH(CH3) (CH2)3-,
(2, 3, or 4)-(alkyl)-C6H4CH(CH3) (CH2)3-, (2, 3, or 4)-(alkyloxy)- C6H4CH(CH3) (CH2)3, (2, 3, or 4)-X-C6H4-alkyl,
(2, 3, or 4) -X-C6H4CH(CH2CH3)CH2-,
(2, 3, or 4)-X-C6H4CH(CH3) (CH2) - 4-X-C6H4CH(CH3) (CH2)2-, and
4-X-C6H4-CH(CH3) (CH2)3-; C6H5CH(OCH3) (CH2)2-,
C6H5CH— CH-CH2-, C6H5CO(CH2)3-, C6H5CO (CH2)4-,
Figure imgf000054_0001
CH2
(2, 3, or 4)-(alkyl)-C6H4CO(CH2)3-,
(2, 3, or 4)-(alkyl-oxy)-C6H4CO(CH2)3-, (2, 3, or 4)-X-C6H4CO(CH2)n-,
2-thienyl-CO-(CH2)3-,
-alkyl-piperazinyl-aryl;
-alkyl-C3-8cycloalkyl-aryl;
-alky 1-piperaz inyl-heterocycle;
-alkyl-C3-8cycloalkyl-heterocycle;
-alkyl-C3-8cycloalkyl-Ar1;
-alkyl-piperazinyl-Ar1;
-alkenyl-piperazinyl-aryl;
-alkenyl-C3-8cycloalkyl-aryl;
-alkyl-aryl-heterocycle;
-alkyl-heterocycle-aryl;
-alkenyl-C3-8cycloalkyl-Ar1;
-alkenyl-piperazinyl-heterocycle;
-alkenyl-C3-8cycloalkyl-heterocycle;
-alkenyl-piperazinyl-Ar1;
Ar1-pH(CH2)n-,
Ar
Figure imgf000054_0002
1
(2, 3, or 4)-X-C6H4C(CH3)CH(CH2)2-, where the conformation about the double bond is cis or trans,
(2, 3, or 4)-X-C6H4C(CH3)CHCH2-, where the conformation about the double bond is cis or trans,
(2, 3, or 4)-X-C6H4COCH=CHCH2-,
Y-CH2(CH2)n-, Ar1-(CH2)n-, C1 to C20 alkyl,
X-(CH2)nCO-, or X-(CH2)n-; R3 = =O, =NH, =S, chloro, bromo, iodo, fluoro, alkyl, or aryl;
n = 1 to 6;
p = 1 to 20;
X = is independently F, Cl, Br, I, OCH3, SO3-,
NH2, H,
-OH, -COOH, -COOR, -SO3H, -CN, -NHSO3H, -NO2, or -SO2NH2;
Y = H, F, Cl, Br, I, -SO3, -PO4 =, -OH, -SH,
-SCH3, -CH3SO2-, -NH2, or -CO2-; and
Ar1 = independently, aryl, (2, 3, or 4-X-C6H4-),
(2, 3, or 4)-(CH2X)C6H4-,
(2, 3, or 4)-(CX3)C6H4-,
(2, 3, or 4)-(CHX2)C6H4-, 2-thienyl, or
(2, 3, or 4 ) -X-C6H4CH2- or its
pharmaceutically acceptable salt, including a quaternary salt, in a pharmaceutically- acceptable diluent or carrier.
43. The composition of claim 42, wherein the alkyl group is selected from the group consisting of CH3-, cyclohexyl, (CH3)2CH-, CH3(CH2)3-,
(CH3)2CHCH2-, CH3CH2CH(CH3)-, -CH3(CH2)p and (CH3)3C-.
44. The composition of claim 42, wherein the aryl group is selected from the group consisting of C6H5-, (2, 3, or 4) - (OCH3) C6H4- and (2, 3, or 4)-
(CH3)C6H4-; 2-X-C6H4-, 3-X-C6H4-, or 4-X-C6H4-.
45. The composition of claim 42, wherein
(2, 3, or 4) -X-C6H4-alkyl- is selected from the group consisting of (2, 3, or 4)-X-C6H4CH(CH2CH3)CH2-, (2, 3, or 4)-X-C6H4CH(CH3) (CH2)-,
(2, 3, or 4)-X-C6H4CH(CH3) (CH2)2-, and
(2, 3, or 4)-X-C6H4-CH(CH3) (CH2)3-.
46. The composition of claim 42, wherein the quaternary ammonium salt is of the formula -NR3 +Z-, wherein R is alkyl or benzyl, and Z is a
counteranion selected from the group consisting of chloride, bromide, iodide, -O-alkyl, toluenesulfonate, methylsulfonate, sulfonate, phosphate, benzoate, succinate, acetate, glycolate, succinate, maleate, malate, citrate, tartrate, ascorbate, benzoate, cinnamoate, mandeloate, benzyloate, and diphenylacetate.
47. The composition of claim 42 in a topical carrier in an amount by weight of between 0.001 and 100% buspirone derivative.
PCT/US1994/003353 1993-03-26 1994-03-28 Topical and systemic application of buspirone or derivatives thereof for treatment of pathological conditions associated with immune responses WO1994022448A1 (en)

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AT94911001T ATE241359T1 (en) 1993-03-26 1994-03-28 TOPICAL AND SYSTEMIC USE OF BUSPIRON AND ITS DERIVATIVES FOR THE TREATMENT OF PATHOLOGICAL CONDITIONS ASSOCIATED WITH IMMUNE RESPONSES
DE69432749T DE69432749T2 (en) 1993-03-26 1994-03-28 TOPICAL AND SYSTEMIC APPLICATION OF BUSPIRON AND ITS DERIVATIVES FOR THE TREATMENT OF PATHOLOGICAL CONDITIONS RELATED TO IMMUNE RESPONSES
CA002159091A CA2159091C (en) 1993-03-26 1994-03-28 Topical and systemic application of buspirone or derivatives thereof for treatment of pathological conditions associated with immune responses
EP94911001A EP0690715B1 (en) 1993-03-26 1994-03-28 Topical and systemic application of buspirone or derivatives thereof for treatment of pathological conditions associated with immune responses
JP6522262A JPH09502699A (en) 1993-03-26 1994-03-28 Treatment of immunoreactive pathologies by local and systemic administration of buspirone or its derivatives
DK94911001T DK0690715T3 (en) 1993-03-26 1994-03-28 Topical and systemic application of busphirone or its derivatives for the treatment of pathological conditions associated with immune reactions
AU63692/94A AU692764B2 (en) 1993-03-26 1994-03-28 Topical and systemic application of buspirone or derivatives thereof for treatment of pathological conditions associated with immune responses

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US08/037,271 US5484788A (en) 1993-03-26 1993-03-26 Buspirone as a systemic immunosuppressant
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US08/037,271 1993-03-26
US08/037,225 US5631017A (en) 1993-03-26 1993-03-26 Topical application of buspirone for treatment of pathological conditions associated with immune responses

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US5637314A (en) * 1995-06-07 1997-06-10 Beth Israel Deaconess Medical Center, Inc. Topical and systemic application of buspirone or derivatives thereof for treating atopic dermatitis
WO1997037659A1 (en) * 1996-04-08 1997-10-16 Sano Corporation Transdermal delivery device for azapirone compounds

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Cited By (3)

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Publication number Priority date Publication date Assignee Title
WO1996006601A1 (en) * 1994-08-30 1996-03-07 Allergan Use of 5-ht ligands as anti-pruritic agents
US5637314A (en) * 1995-06-07 1997-06-10 Beth Israel Deaconess Medical Center, Inc. Topical and systemic application of buspirone or derivatives thereof for treating atopic dermatitis
WO1997037659A1 (en) * 1996-04-08 1997-10-16 Sano Corporation Transdermal delivery device for azapirone compounds

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