WO1994003619A3 - Improved plant germplasm - Google Patents

Improved plant germplasm Download PDF

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Publication number
WO1994003619A3
WO1994003619A3 PCT/GB1993/001605 GB9301605W WO9403619A3 WO 1994003619 A3 WO1994003619 A3 WO 1994003619A3 GB 9301605 W GB9301605 W GB 9301605W WO 9403619 A3 WO9403619 A3 WO 9403619A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene
expression
plant
application
inducer
Prior art date
Application number
PCT/GB1993/001605
Other languages
French (fr)
Other versions
WO1994003619A2 (en
Inventor
Simon William Jonathan Bright
Andrew James Greenland
Ian Jepson
Jacqueline Ann Mary Paine
Original Assignee
Zeneca Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zeneca Ltd filed Critical Zeneca Ltd
Priority to EP93917945A priority Critical patent/EP0658207B1/en
Priority to AT93917945T priority patent/ATE224955T1/en
Priority to CA002140267A priority patent/CA2140267A1/en
Priority to AU47184/93A priority patent/AU687008B2/en
Priority to DK93917945T priority patent/DK0658207T3/en
Priority to DE69332338T priority patent/DE69332338T2/en
Publication of WO1994003619A2 publication Critical patent/WO1994003619A2/en
Publication of WO1994003619A3 publication Critical patent/WO1994003619A3/en
Priority to US11/038,228 priority patent/US20050229269A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Hydroponics (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

A recombinant plant genome contains a gene cascade such that it is incapable of producing a mature plant but requires the presence of a chemical inducer. The gene cascade includes a gene switch which is inducible by external application of a chemical inducer and which controls expression of a gene product which affects expression of a second gene in the genome. Survival or development of the plant is dependant upon either expression or non-expression of the second gene and application of the inducer, therefore, selects whether or not the plant develops. In one example the second gene encodes a cytotoxic molecule and expression of that gene is fatal to the plant. In another example the second gene encodes a desirable characteristic which may be excised selectively by applying or withholding chemical application. The second gene may be placed under control of a development regulated promoter.
PCT/GB1993/001605 1992-07-29 1993-07-29 Improved plant germplasm WO1994003619A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP93917945A EP0658207B1 (en) 1992-07-29 1993-07-29 Method for the genetic containment of plants
AT93917945T ATE224955T1 (en) 1992-07-29 1993-07-29 METHOD FOR THE GENETIC CIRCULATION OF PLANTS
CA002140267A CA2140267A1 (en) 1992-07-29 1993-07-29 Improved plant germplasm
AU47184/93A AU687008B2 (en) 1992-07-29 1993-07-29 Improved plant germplasm
DK93917945T DK0658207T3 (en) 1992-07-29 1993-07-29 Method for genetic containment of plants
DE69332338T DE69332338T2 (en) 1992-07-29 1993-07-29 METHOD FOR GENETICALLY CIRCULATING PLANTS
US11/038,228 US20050229269A1 (en) 1992-07-29 2005-01-21 Plant germplasm

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB929216151A GB9216151D0 (en) 1992-07-29 1992-07-29 Containment of plant germplasm
GB9216151.2 1992-07-29

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US09/045,881 Continuation-In-Part US6605754B1 (en) 1992-04-13 1998-03-17 DNA constructs and plants incorporating them
US40583999A Continuation 1992-07-29 1999-09-27

Publications (2)

Publication Number Publication Date
WO1994003619A2 WO1994003619A2 (en) 1994-02-17
WO1994003619A3 true WO1994003619A3 (en) 1994-03-31

Family

ID=10719517

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1993/001605 WO1994003619A2 (en) 1992-07-29 1993-07-29 Improved plant germplasm

Country Status (11)

Country Link
US (1) US20050229269A1 (en)
EP (1) EP0658207B1 (en)
AT (1) ATE224955T1 (en)
AU (1) AU687008B2 (en)
CA (1) CA2140267A1 (en)
DE (1) DE69332338T2 (en)
DK (1) DK0658207T3 (en)
ES (1) ES2181692T3 (en)
GB (1) GB9216151D0 (en)
PT (1) PT658207E (en)
WO (1) WO1994003619A2 (en)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69331055T2 (en) 1992-04-13 2002-06-20 Syngenta Ltd DNA CONSTRUCTIONS AND PLANTS CONTAINING THEM
EG23907A (en) * 1994-08-01 2007-12-30 Delta & Pine Land Co Control of plant gene expression
US5723765A (en) * 1994-08-01 1998-03-03 Delta And Pine Land Co. Control of plant gene expression
AUPN442295A0 (en) * 1995-07-26 1995-08-17 Commonwealth Scientific And Industrial Research Organisation Regulated autocrine growth of mammalian cells
CA2234557C (en) * 1995-10-13 2007-01-09 Purdue Research Foundation Method for the production of hybrid plants
GB9606062D0 (en) 1996-03-22 1996-05-22 Zeneca Ltd Promoters
AUPN903196A0 (en) * 1996-03-29 1996-04-26 Australian National University, The Single-step excision means
AR006928A1 (en) 1996-05-01 1999-09-29 Pioneer Hi Bred Int AN ISOLATED DNA MOLECULA CODING A GREEN FLUORESCENT PROTEIN AS A TRACEABLE MARKER FOR TRANSFORMATION OF PLANTS, A METHOD FOR THE PRODUCTION OF TRANSGENIC PLANTS, A VECTOR OF EXPRESSION, A TRANSGENIC PLANT AND CELLS OF SUCH PLANTS.
WO1997044465A1 (en) * 1996-05-20 1997-11-27 Monsanto Company Method for controlling seed germination using soybean acyl coa oxidase sequences
EP1006780A4 (en) * 1997-01-24 2005-03-09 Dna Plant Techn Corp Two component plant cell lethality methods and compositions
US6392119B1 (en) 1997-01-24 2002-05-21 Dna Plant Technology Corporation Two component plant cell lethality methods and compositions
CN1262660C (en) * 1997-07-30 2006-07-05 辛甄塔有限公司 Genetic method for controlling sprouting
US6632980B1 (en) 1997-10-24 2003-10-14 E. I. Du Pont De Nemours And Company Binary viral expression system in plants
CA2319110C (en) * 1998-02-20 2011-01-04 Zeneca Limited Hybrid seed production
GB9817909D0 (en) * 1998-08-17 1998-10-14 Zeneca Ltd DNA constructs
GB2355459B (en) * 1999-11-29 2001-09-26 Isis Innovation A dominant conditional lethal genetic system
US6849776B1 (en) * 2000-07-14 2005-02-01 Unicrop Ltd Molecular control of transgene segregation and its escape by a recoverable block of function (RBF) system
US20020007500A1 (en) * 2000-07-14 2002-01-17 Viktor Kuvshinov Molecular control of transgene escape by a repressible excision system
US7847063B2 (en) 2003-03-28 2010-12-07 Sanwa Kagaku Kenkyusho Co., Ltd. GLP-1 derivative
GB2404382B (en) 2003-07-28 2008-01-30 Oxitec Ltd Pest control
GB2443186A (en) 2006-10-25 2008-04-30 Oxitec Ltd Expression system for mediating alternative splicing
CA2669453C (en) 2006-08-04 2018-11-13 Verenium Corporation Glucanases, nucleic acids encoding them and methods for making and using them
PT2617823E (en) 2006-09-21 2015-11-23 Basf Enzymes Llc Phytases, nucleic acids encoding them and methods for making and using them
EP2479266B1 (en) 2006-12-21 2016-03-09 BASF Enzymes LLC Amylases and glucoamylases, nucleic acids encoding them and methods for making and using them
MX2009008210A (en) 2007-02-02 2009-08-12 Pioneer Hi Bred Int Selective ablation of diploid embryos.
EP2698374B1 (en) 2009-05-21 2016-09-14 BASF Enzymes LLC Phytases, nucleic acids encoding them and methods for making and using them
CA2811789A1 (en) 2010-10-06 2012-04-12 Bp Corporation North America Inc. Variant cbh i polypeptides with reduced product inhibition
CN102181463A (en) * 2011-02-25 2011-09-14 北京农业生物技术研究中心 Marker gene induction deleting system based on AlcR/alcA and Cre/loxP system
GB2500113A (en) 2012-03-05 2013-09-11 Oxitec Ltd Arthropod male germline gene expression system
GB201303932D0 (en) 2013-03-05 2013-04-17 Oxitec Ltd Muscle actin promoter
GB2526867A (en) 2014-06-05 2015-12-09 Oxitec Ltd Gene expression system
WO2018029534A1 (en) 2016-08-12 2018-02-15 Oxitec Ltd. A self-limiting, sex-specific gene and methods of using

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0332104A2 (en) * 1988-03-08 1989-09-13 Ciba-Geigy Ag Chemically regulatable DNA sequences and genes and uses thereof
WO1990008830A1 (en) * 1989-01-26 1990-08-09 Imperial Chemical Industries Plc Hybrid seed production
WO1990008828A2 (en) * 1989-02-02 1990-08-09 Paladin Hybrids Inc. Molecular methods of hybrid seed production
EP0412006A1 (en) * 1989-08-04 1991-02-06 Plant Genetic Systems, N.V. Plants with modified flowers, seeds or embryos
WO1991003561A1 (en) * 1989-09-11 1991-03-21 E.I. Du Pont De Nemours And Company Expression of herbicide metabolizing cytochromes p450
WO1991009957A1 (en) * 1989-12-22 1991-07-11 E.I. Du Pont De Nemours And Company Site-specific recombination of dna in plant cells
WO1992001799A1 (en) * 1990-07-20 1992-02-06 Paladin Hybrids Inc. Binary cryptocytotoxic method of hybrid seed production
EP0494724A2 (en) * 1991-01-08 1992-07-15 Institut Für Genbiologische Forschung Berlin Gmbh Plasmids for controlling expression in plants

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0332104A2 (en) * 1988-03-08 1989-09-13 Ciba-Geigy Ag Chemically regulatable DNA sequences and genes and uses thereof
WO1990008830A1 (en) * 1989-01-26 1990-08-09 Imperial Chemical Industries Plc Hybrid seed production
WO1990008828A2 (en) * 1989-02-02 1990-08-09 Paladin Hybrids Inc. Molecular methods of hybrid seed production
EP0412006A1 (en) * 1989-08-04 1991-02-06 Plant Genetic Systems, N.V. Plants with modified flowers, seeds or embryos
WO1991003561A1 (en) * 1989-09-11 1991-03-21 E.I. Du Pont De Nemours And Company Expression of herbicide metabolizing cytochromes p450
WO1991009957A1 (en) * 1989-12-22 1991-07-11 E.I. Du Pont De Nemours And Company Site-specific recombination of dna in plant cells
WO1992001799A1 (en) * 1990-07-20 1992-02-06 Paladin Hybrids Inc. Binary cryptocytotoxic method of hybrid seed production
EP0494724A2 (en) * 1991-01-08 1992-07-15 Institut Für Genbiologische Forschung Berlin Gmbh Plasmids for controlling expression in plants

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KARLIN-NEUMANN, G.A., ET AL.: "Phytochrome control of the tms2 gene in transgenic Arabidopsis: A strategy for selecting mutants in the signal transduction pathway", THE PLANT CELL, vol. 3, no. 6, June 1991 (1991-06-01), pages 573 - 582 *

Also Published As

Publication number Publication date
ATE224955T1 (en) 2002-10-15
WO1994003619A2 (en) 1994-02-17
DK0658207T3 (en) 2003-02-03
AU4718493A (en) 1994-03-03
EP0658207A1 (en) 1995-06-21
CA2140267A1 (en) 1994-02-17
EP0658207B1 (en) 2002-09-25
DE69332338D1 (en) 2002-10-31
US20050229269A1 (en) 2005-10-13
PT658207E (en) 2002-12-31
ES2181692T3 (en) 2003-03-01
GB9216151D0 (en) 1992-09-09
DE69332338T2 (en) 2003-02-13
AU687008B2 (en) 1998-02-19

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