WO1993006234A1

WO1993006234A1 - Method of cleaning soil polluted with oil and petroleum products

Info

Publication number: WO1993006234A1
Application number: PCT/RU1992/000174
Authority: WO
Inventors: Olga Nikolaevna Antropova; Svetlana Lvovna Leu
Original assignee: Olga Nikolaevna Antropova; Svetlana Lvovna Leu
Priority date: 1991-09-19
Filing date: 1992-09-18
Publication date: 1993-04-01
Also published as: RU2049739C1

Abstract

A method of cleaning soil polluted with oil and petroleum products comprises determination of the pollution degree of the area by means of boring holes and pumping in solutions of surface-active substances or a medium for cultivation of micromycetes. A suspension of nutritive substances, such as salts containing cations of ammonium, potassium, magnesium, ferrum (III) and anions of nitrate, phosphate and sulphate, are then introduced in the soil both on and below the surface to a depth of 1.5 metres. Microorganisms used are at least one oil-assimilating species or strain of soil micromycetes of the genus Penicillium, Mucor, Aspergillus, Trichoderma. The suspension is pumped in the soil at a fixed pressure and at a certain ratio of the quantity of spores per unit of oil pollution.

Description

SPECIAL BOARD

DISCUSSION OF THE INVENTION The invention is not limited to the use of micro-friendly utilization of 5 contaminated products, but more to the contrary, κ. The method of calculating the water and the waste of oil and food.

THE PRESENT VERSION OF THE FIRST TIME AND THE EASTERN RESOURCES

10 substances by introducing into soil contaminated soil soil specific microorganisms-bacteria of the genus Ι Ρ Ρ Ι г г η аз аз аз; form of ^'one- piece suspension or fixed on a commercially available medium. on η- example, whole, silica gel, active carbon. The organic matter is azhany, aldehydes, and non-cyclic substances.

15 hydrocarbons, processing phenols are in the presence of water, acid or mineral salts (ΒΕ, Α1, 3545325).

Used in the described method of the bacterium is a char- acterized by a functional system that is connected to a mainstream hydrocarbon What is the scope of the action?

20 bacteria limit the possibility of widespread use of agricultural machinery and any other contaminated soil.

There is also a known reason for the addition of food and other things to avoid, which includes the application of foreign currencies. for example, an external one. susceksi ;:; ^" -: mixtures of a specific microorganism. In addition ^, they use the culture of the Republic of Belarus ::: rzlαz-ZS. and mineral fertilizer-nitro ammo (5ϋ, SS, 1428609 ;.

In this way, the introduction of components is dried out by increasing the number of participants who have a low degree of activity.

30 (d- ^~ ιο kg per square meter). Therefore, it can only be used for the most important soil contamination. Κaκ ποavi- hp, πρi zagρyazneniκ vοdy and ποchvy neατyu, gοelednyal legκ ^{"προκiκaο?} Gιο gορκzοnτu on gluοinu οοl te ι meτρa.

The main part of the world is easy to share. ^”

MH-aromatherapy phrases. This data, including the bacterium, isn’t effective for the destruction of aromatic compounds; call the com - 2 - an expression that deeply penetrates at a depth of heavy parts of the system and remains unworked and, therefore, the use of the terms and conditions of use is prohibited. 5 yzveeτen sποsοb liκvidatsii neφτyanοgο zagρyazneniya ποchvy, πρedusmaτρivayuschy φορmiροvanie, πο at meρe, οdnοgο aeρο zοlnοgο οblaκa, sοdeρzhaschegο πiτaτelnye veschesτva and miκροορ-organisms: zhbο baκτeρii ροdοv Α ^ ^• ^^^ al ^ e ^, Νοsagsϋa. Freedom of Friendship of the Republic of Kazakhstan, for the activities of the activists of the Soviet Union (ΙΕ, С, 10 4136024).

Ав Having a healthy environment, it is agreed to this: seawater, raw oil, ferrous extract, sulphate and disubstituted.

In this way, the cloud is expired due to fixed time - 15 are left behind on the contaminated land. This is convenient for enhancing the number of microorganisms, while others are sufficient for the destruction of non-hazardous pollution.

In the same way as in the previous method, applying the active agent is carried out only on the basis of the contaminated soil, that

£ 0 assures the time of falling down a little to the weight of the heavy fractions of the net, which in turn reduces the scope of the exchange.

ΡΑСΚΡЫΤИΕ IZΟΕΡΕΤΕΗIA

Ε5 Β οsnοvu dannοgο izοbρeτeniya ποlοzhena task sοzdaniya sποsοba οchisτsh ποchvy sτ zagρyazneny neφτyu not © τeπροduκτami πuτem ποdbορa sοοτveτsτvuyuschey τeχnοlοgichesκοy ποsledοvaτel- nοsτi egο sτady, sπetsiφichesκiχ miκροορganizmοv with shiροκim sπeκτροm φeρmenτaτivnοy aκτivnοsτi πο around sπeκτρu neφτyan :: -:

30 components that would greatly increase the number of contaminants: -: partly due to the risk of damage to the patient and the patient -

Pοsτavlennaya task dοeτigaeτsya τem, chτ ^g - s sποsοό- ΟCHYSΤΚY ποchvy οτ zagρyazneniya neφτyu and nesρτeπροduκτam ::.

What is the epidemic? .. Adding ποchvu susπenzi "MiG ροορganizmο vκlyuchayuschey πiτaτelnye veschesτva eοdeρzhaiκe κa ΤYΟΗY ammοniya κazhya and aniοny shοsφaτa and sulshaτa, eοglas ^'n':. valvlennοmu izοόρeτeniyu in κachesτve miκροορganizmοg YZΠΟLZUΓ:

- --- _. * .---. ΤΤΤ ΤΪΤ. - .- ^ ν .ν--. g'-. "LEARNED ττ g ^^ τ -. _' --- tgt ϊ.τ-gtttgt ttthttt ττ tgt.-. tttgg; --Ϊ .-. ; - ' If you select a chain that has been removed from a waste machine, it is safe to use it, but it is preferable that the strain is not used. TsΜPΜ Ρ-107.

Οπρedelenie ρasπροsτρaneniya neφτyanοgο zagρyazneniya πο gο- ρizοnτu ποchvy οsuschesτvlyaeτsya πuτem buρeniya sκvazhin and zaκachκi vοdnyχ ρasτvοροv ποveρχnοsτnο-aκτivnyχ veschesτv dο vyτesneniya on ποveρχnοsτ ποchvy οbρazuyuscheysya vοdnοy neφτeπρο- duκτοv emulsion.

The introduction of an external suspension of micromycetes and nutrients may be at a pressure of 0.4–0.8 PSa, and if the suspension is inadequate, it will not be discharged.

Аче In terms of nutrition, it is possible to use saline, which is more than magnesium, iron (III;), and anionic acids.

The posed problem is solved also and, moreover, that in the aqueous suspension of micronutrients and nutritional substances are added from the calculation from 0.4 to 1 liter of suspension per 1 quarter. m soil, but not less than 2.8-10

pollution. One of the variants of the method can also be that the main suspension of micronutrients and foodstuffs is paid at a cost of 100% of the cost of income. 1 cc.> • soil, but not less than 2.8-10 cpi per 1 ml. casualty; --- neniya.

BETTER TO DELIVER THE INVENTION The proposed method for calculating soil pollution and non-food products is subject to the following process.

In the case of soil, it eliminates the spread of contamination and obstruction, introduces a suspension of absorptive substances. containing a culture of lesser amounts of micromycete, and, as a part of healthy substances, they consume salt containing ammonia, potassium, magnesium and iron. Suspension is introduced into the soil under pressure 0.4-0 ,. ^' .-. W from the calculation of 0.4-1 liter of suspension per 1 quarter. m and 80-100 lit. N.: -: 1 cub. method of soil, but not less than 2.8 * 10 at a rate of 1 milliliter of non-heavy pollution.

After that, the control panel for the products is falling ^ - - inadequate and inadequate products for the establishment of the necessary benefits of the suspension suspension.

Of all the groups of microorganisms, micromycetes are the most stable to technological pollution; they have a large

5 operational system, t. e. we are lucky with a wide range of enzymes, abundant formation. Disposal of contaminated parts of household substances and active non-disinfecting strain leads to the spread of oil or non-discharges. Indicated culture of micromycetes or micromycetes (endorsement) promotes

10 only aromatic and cyclic compounds, using them as the only source of carbon. A small part of the world justifies the assimilation of the products, and therefore non-cyclical products of decay, are inaccessible to the human body.

15 that speeds up the calculation process.

Аче In the quality of mixtures, you can use the mixtures of products of the United States, the United States, the United States, the United States, and the United States. From the source of ,еηϊсϊШшшη, however, the following should be given to the strain of the United States TsΜPΜ Ρ-107. 20 Please note that the use of a mixture of the named / micromaterial or any other type of accident or accident is necessary for the use of the mixture;

Ορeda for κulτiviροvaniya miκροmitseτοv eτο πο suτi mο £ G dishitsiροvannaya sρeda Chaπeκa in κοτοροy οτsuτsτvueτ saχaροza, ποsκοlκο isτοchniκοm ugleροda in dannοm case πρi making miκροmitseτοv with πiτaτelnοy sρedοy in ποchvu isτοchniκοm ugleροda yavlyaeτsya samο neφτyanοe zagρyaznenie. Having compounded the environment for the cultivation of miracles, it contains: nitrate, disubstituted-3? high phosphate, potassium sulfate, and potassium chloride. sulphate of iron, crude oil.

The proposed method may be used with a simple and deep contamination of the soil with crude and inadequate products. In the event of an increased pollution, it is recommended that: • “: - ka 1 kb. m - 0, -1 liter of the aqueous suspension containing not less than 10 doses per 1 ml ... and nutrients not less than 6 grams per liter. If you are comfortable contaminating UHF, you must deeply blow it up to create optimal living conditions.

χ ~ ρ - *. τνгιиΛ. _* ichtgch .., D ττg τ-t-yyyy. ~ _Πχ.иΛΤχΪ τ ιτχил-тчтЬτντут гг> υ * child τ? Ρ .---. Τ Τ Τ Τ Τ ч / _* τ

- 5 - suspensions. Conventional content of oil and non-product in soil does not exceed 15 mass. % Therefore, a specified amount of suspension ensures maximum effective cost and minimal economic costs. For οπρedeleniya κοlichesτva susπenzii neοbχοdimοgο for inclusion in ποchvu usτanavlivayuτ neφτenasyschennοsτ ποchvy, for chegο προbuρivayuτ ρyad sκvazhn and zaκachivayuτ in niχ ρasτvορy πο veρχnοsτnο-aκτivnyχ veschesτv dο vyτesneniya on ποveρχnοsτ ποch you οbρazuyuscheysya πρi eτοm vοdnοy emulsion or neφτi neφτeπρο duκτοv. For the radius of the dispersal of this emulsion, I share the required number of bore holes. B ^s' avisimοsτi οτ sτeπe any depth and προniκnοveniya zagρyazneniya κοlichesτvο sκvazhin mο zheτ dοsτigaτ shτuκ 30-40 per 1 ha.

Аче On the other hand, a non-oiling agent can use a medium for cultivating mixtures.

It is advisable to pump 60-80 liters of food from a well into a well. After the separation of the contamination of the soil, the soil will have a costly effect of not incurring a loss of emissivity. m πρ and pressure 0.4-0.8 ΜPa. Pressure οπρedelyaeτsya glubinοy προniκnοveni agρyazneniya, sοsτavlyayuschey οbychnο οτ 20 cm dο 1.5 m .. Κοli- chesτvο vοdnοy susπenzii οπρedelyaeτsya saturation πο ^'chvy neφgκ or neφτeπροduκτami and its τaκzhe sτρuκτuροy. After working on contaminated soil, contacting the consumer for products of decay of oil or for foodstuffs for food wastes The environment for the decay products is carried out by chemical and spectral analyzes in general during the process of calculation. The process of calculating itself is available from 0.5 to 1 year, the addition of a suspension can be completed 2-3 times in the process of calculating.

For a better understanding of this invention, the following are examples of its intended use.

For example, 1.

Arable land with an area of 20 ha is contaminated with oil in the amount of 4,000 tons as a result of a malfunction accident. Ρasπροst- ά.ΗеΗйЬ ΗβψΤИ ϊϊυ ΙЮБсφлΚϋϋ`Ι'η ϋϋрЬД Д ЛΗЛй -зΜЗ_у ^τ Шά ±> ηϋ, Η ύϋ ϊ'ϋ π- - 6 - to an area by drilling 650 wells on 20 hectares. Β At the bottom, 60-80 liters were pumped. Mineral salt flavors that have the following ingredients (grams per bar): ΝаΝ0 ₅ - 3.0 ΚзΗΡΟ * - 2.0 5 1 ^ 30 ^ 7-Η _. gΟ - 0.5 ΚСΙ - 0.49 Ρе50 _ ,? Η ₂ 0 - 0.01

Due to the fact that the external emulsion of oil was displaced at the turn, the polluted areas were separated. A contamination of 10 was detected at a depth of about 0.6 to 1.5 meters. Contributed participants were allowed to deep dry and were exposed to fresh mineral salts indicated above the composition of 10000 l. on 20 hectares.

We received an honorable request that we have sent a 15th part of the contract by means of the irrigation of a foreign complaint, which is a major part of the market. Ι ШПΜ Ρ-107 of 1 liter. by 1 square. m

per 1 ml. o / For two months after the perevnichnuyu osobennosti the content of the oil, a large amount of chemical imbalance in: 00 increased by 8% from a decrease of 10-15%. There was a second processing of a single suspension.

After 0.5 year, the wells were drilled to a depth of 0.5 d-; 1.5 m. The number of boreholes was 350. The final output was 2- dividing the external suspension by the soil at a pressure of 0.4-0.3 psi. The introduction of the external suspension is even deeper than 0.6-0.7 m. It was maintained at a pressure of 0.5-0.0 Pa. a depth of 0.7-1.5 m πρ and a pressure of 0.0 ΜPa. The greater the number of days, the suspension in the bottom bore was 60-100 liters. Dependent on G-St ---. _τ ___ _Г π-уин .. Radius of the expansion of the suspension _._.- • - ^{• "} : k. ε> - BySϊ-g? ον pressure of its delivery to be quashed. There was an out-of-operation part: 1 ha. suepenzy ^" . content of 100-! 23 kg mi-internationals>: salt.

Over the course of the year, after a successful and deep installation, the device has lost 80 to 90 φ net. content in the soil at a depth of 1.5 m. The prevalence of the rest has become loose. easy to enjoy --- lasϊ *. Part of the land was planted with baked goods, part of Sudan ^' :. Bx--

ЖСΪЬ 100, for the most outward look The same kind of - 7 - not contaminated with power.

For example, 2.

Β biοlοgichesκie maτρatsy ποmeschali ποchvy 200 g and 20 ml .. neφτi and sποροvuyu susπenziyu gρibοv ΑzρegeShig atzϊeϊasϊaη .. Sποροvaya susπenziya vnοsilas ρascheτa of 2.8 ^* 10 mL sπορ 1 .. neφτi. Over the course of 15 days, the temperature was constant at 27 = 27 ° C, 85% of which was not utilized. The similar results were obtained by applying a miracle £ exceeded £ _11 from ϊ ϊ ϊ ϊ ϊ.. 0 ρ example ρ 3.

Β biological matrices for 200 g. Soils introduced 20 ml .. neft. After that they introduced a suspense _. content 2, 8 “10 sπορ Τгϊсуосϊегта кοηϊдϊ. Over the course of 15 days, 87% of the net waste has been disposed of. 5

Example 4.

Β biοlοgichesκie maτρatsy vnοsili ποchvy 200 g and 20 ml .. neφτi and sποροvuyu susπenziyu ΡeηϊsShϊit zζϊzοeηshτi For Ιδ-τi suτοchny πeρiοd τeρmοsτaτiροvaniya uτiliziροvanο 96% neφτi.0 Αnalοgichnye ρezulτaτy ποlucheny with miκροmitseτοm Ρeη-C * Shiιτ

Example 5.

200 Biological materials for 200 g. Soil contributed 20 ml. 5 crude and impaired suspension of mushroom gas hoses in the same case., Including 2-4. After 15 days of incubation, 97% of the net was developed.

Example 6. 0 ма biological matrices for 200 g. Soil weighed 20 ml. . oil and competitive suspension of micromycetes; ΑΑρегегд ^{* *} . aiggBϊeΙasΙaηϊ, ΑΑρρегегШизиз изизρρϊϊϊгг,, Τηсηοсϊеггη: - τ с * ___ And * _ - .-. . -d ... w XX .ϋ ^* % <* -. υ _-чι ^* > -. - ινч <• ____ ι. <g "* -_ С __? _, уΙгπν- ^* -. - * - and -. * g *" * ι I Τ

for 1 ml .. oil. Due to the daily incubation, 99.5% of the net was disposed of. - 8 -

Example 7.

In the case of natural conditions, and for the contamination of 1 year of oil for 1 year, the soil is in good condition as a result of the fact that there is no risk of waste. ϊегϊϊϊϊ, ,Τ, ΤΤϊЬЬάάάтата к кο ϊϊϊϊϊ ΡΡΡϊϊϊϊϊϊϊϊϊ

Ηеη. ηοτ: and £ ιιt, the news from the newspaper was reduced for 1 year 63% of the net.

PΡΟSHPSHΕΗΗΑYA ΙΡΜΜΕΗIΜΟSΤ Pρedlοzhenny sποsοb mοzheτ nayτi πρimenenie for οchisτκi and οόezzaρazhivaniya vοdy and ποchvy in zοnaχ naχοzhdeniya οbeκτοv neφ- τyanοy and neφτeπeρeρabaτyvayuschey προmyshlennοsτi and τaκzhe dρugiχ οbeκτaχ, isποlzuyuschχ neφτeπροduκτy.

The use of the proposed method ensures an efficient calculation of the soil with a degree of loading of more than 10 percent over a depth of 2 meters.

Claims

- 9 -ΦΡΜΡΜΡΜΑΑ ΟΟΟΡΕΤΕΗΡΕΤΕΗ

1. Sποsοb οchisτκi ποchvy οτ zagρyazneniya neφτyu and neφτeπ- ροduκτami, πρedusmaτρivayuπschy πeρiοdichesκοe entering into ποchv susπenzii miκροορganizmοv vκlyuchayuschey πiτaτelnye veschesτva, sο- deρzhaschie κaτiοny ammοniya, and κaliya aniοny φοsφaτa and sulφaτa, οτzhchayuschiysya τem, chτο in κachesτve miκροορganizmοv isποlzuyuτ πο at meρe οdin neφτeusvaivayuschy view of the strain of mixtures selected from the facilities of исegisiШit, ΜΜοοΑ, ρΑρегегегάизтататататата 0тата, 0 ΤтаϊтаЬάάтататататата.

2. The method of clause 1 is due to the fact that, as a result of the poorly assimilating strain, the strain is used. TsΜP Ρ-107.

3. Sποsοb πο π.1 οτlichayuschiysya τem, chτο οπρedelyayuτ 'ρasπ-5 ροsτρanenie neφτyanοgο zagρyazneniya πο gορizοnτu ποchvy πuτem buρeniya sκvazhin and zaκachκi vοdnyχ ρasτvοροv ποveρχnοsτnο-aκ- τivnyχ veschesτv dο vyτesneniya on ποveρχnοsτ ποchvy οbρazuyu- scheysya vοdnοy neφτeπροduκτοv emulsion.

4. The method of production of items 1, 3 is different in that the introduction of one suspension of micromycetes and nutritional substances is under pressure 0.4–0.8 PSa.

5. The method of production is 1, 3, 4, 5, which is different from the introduction of an external suspension in the soil of it, which leads to the loss of healthy substances. 5

6. The method of production is characterized by the fact that, as a part of healthy substances, they use saline containing magnesium, iron (III), and nitrate anion.

7. The method of production is due to the fact that a single suspension of micromycetes and nutritional substances is added from the calculation of 0.40 to 1 liter of suspension per 1 quarter. there is at least 2.8 • 10 ^{5 "} speed per 1 ml of non-hazardous contamination. δ. This is just one shortfall, which results in a loss of 80% of the body Good soil, but not less than 5 2.8 * »10 ⁵ spi at 1 ml of non-toxic contamination.