WO1993000434A1 - Capsid forming and cystein modified chimaeric ms2-coat protein - Google Patents

Capsid forming and cystein modified chimaeric ms2-coat protein Download PDF

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Publication number
WO1993000434A1
WO1993000434A1 PCT/GB1992/001159 GB9201159W WO9300434A1 WO 1993000434 A1 WO1993000434 A1 WO 1993000434A1 GB 9201159 W GB9201159 W GB 9201159W WO 9300434 A1 WO9300434 A1 WO 9300434A1
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Prior art keywords
protein
chimaeric
cysteine residue
coat protein
protein according
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PCT/GB1992/001159
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French (fr)
Inventor
Robert Allan Mastico
Peter George Stockley
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British Technology Group Ltd.
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Publication date
Application filed by British Technology Group Ltd. filed Critical British Technology Group Ltd.
Priority to DE69232069T priority Critical patent/DE69232069T2/en
Priority to AU21955/92A priority patent/AU657560B2/en
Priority to US08/167,982 priority patent/US5698424A/en
Priority to EP92913894A priority patent/EP0591369B1/en
Priority to AT92913894T priority patent/ATE205880T1/en
Priority to JP50123093A priority patent/JP3514755B2/en
Priority to KR1019930703986A priority patent/KR100222163B1/en
Publication of WO1993000434A1 publication Critical patent/WO1993000434A1/en
Priority to FI935877A priority patent/FI110613B/en
Priority to NO19934842A priority patent/NO315050B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
    • A61K47/549—Sugars, nucleosides, nucleotides or nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
    • C12N2760/00011—Details
    • C12N2760/16011—Orthomyxoviridae
    • C12N2760/16022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Definitions

  • This invention relates to a chimaeric protein and is particularly directed to virus proteins containing foreign
  • 10 protein carriers can be specifically directed so that foreign epitopes are reliably presented on the surface of the protein capsid assembly after the expression of the chimaeric protein in a bacterial host.
  • copending Application No. 9201372.1 relates to a chimaeric protein, capable of forming part of a capsid assembly and comprising the amino acid sequence of the coat
  • a chimaeric protein can be expressed in a suitable bacterial host to yield capsids empty of the phage RNA and largely free of other nucleic acid contaminants. While it is contemplated that the insertion of a foreign epitope which is a linear polypeptide of comparatively short length, for example up to about 30 amino acid residues, can be accomplished, it will be appreciated that the presentation of non-linear epitopes by simple insertion in the ⁇ -hairpin may not be feasible. Thus many epitopes do not consist of simple linear fragments of polypeptide : instead they are made up of several such fragments spatially related by the overall three-dimensional folding of the native protein antigen, i.e. discontinuous epitopes. It is clearly desirable to be able to present such epitopes at the surface of the phage capsid.
  • the coat protein of MS-2 can be employed with a view to presenting a range of epitopes or other molecular species such as targeting moieties at the capsid surface by removing the cysteine residues which occur at sites not included within the N-terminal ⁇ -hairpin and introducing a cysteine residue within the ⁇ -hairpin region, which cysteine residue is then available for linking with desired antigens or other molecular species, suitably via an appropriate spacer.
  • a chimaeric protein capable of forming part of a capsid assembly and comprising the amino acid sequence of the coat protein of phage S-2, or a conservatively modified variant thereof, or sufficient of said sequence or variant to retain the capability of forming a capsid assembly, which amino acid sequence has been modified by removal of the cysteine residues present externally of the N-terminal protruberant ⁇ -hairpin of the coat protein and insertion of a cysteine residue within the region corresponding to the N-terminal protruberant ⁇ -hairpin.
  • the cysteine residues external to the ⁇ -hairpin are suitably removed by mutation at the appropriate sites of the coat protein cDNA.
  • the inserted cysteine may be linked directly to a desired molecular species to be presented such as an epitope, especially a non-linear antigenie protein, or to other molecular species to be presented such as a targeting moiety.
  • a spacer moiety may be employed between the cysteine and the molecular species to be presented, thus extending the range of species that can be presented.
  • Suitable spacer moieties are selected from known commercially available heterobifunctional crosslinking reagents which couple with the exposed cysteine thiol group.
  • cross-linkers are m-maleimidobenzoyl-N-hydroxy- sulfosuccini ide ester, N-succinimidyl-(4-iodoacetyl)amino- benzoate and N-succinimidyl-3- ( 2-pyridyldithio)propionate.
  • the choice of crosslinker will depend on the molecular species to be presented and its size. Thus larger molecular species may require longer crosslinking moieties to minimize steric hindrance.
  • the cross!inker may be linked first to the cysteine residue or first to the molecular species to be presented.
  • the chimaeric coat protein is preferably that derived from phage MS-2, but it may also be derived from related RNA-phages capable of replication in E. coli t such as phages R17, fr, GA, Q ⁇ and SP.
  • RNA-phages of physical structure similar to that of MS-2 will contain some chemical variation in the amino acid residues of the coat protein and are thus conservatively modified variants of MS-2 coat protein. While it is believed at present that substantially the entire coat protein may be required for capsid assembly, deletions and/or insertions of a relatively minor nature may also be possible whilst still retaining capsid-forming capability. Proteins having such modified sequences are included within the scope of the invention.
  • the foreign moiety is inserted at the region of the protein which in the assembled capsid corresponds to the N-terminal ⁇ -hairpin.
  • the three-dimensional structure of the MS-2 phage particle has been published by Valegard e ⁇ al-, (Nature, 1990, 245, 36-41). The published data show that, firstly, the structure of the coat protein is not related to the eight-stranded ⁇ -barrel motif found in all other spherical RNA virus subunits whose structures are known at the present time. Secondly, although the coat protein exhibits quasi-equivalent inter-subunit contacts, there are no other devices, such as extended arms of polypeptide, helping to secure each protein conformer. The coat protein structure can be viewed in terms of three separate regions.
  • the present invention comprises modification of the coat protein amino acid sequence by introduction of a cysteine residue in the region corresponding to the protruberant hairpin.
  • the chimaeric protein of the invention has therefore been so modified in the region of amino acid residues 1 to 20, such numbering being with reference to the entire coat protein sequence of MS-2 as published by Fiers, Nature, 1976, 260, 500-507.
  • the modification to introduce the cysteine residue is towards or at the middle of the hairpin region. It is preferred to introduce the cysteine in the region of the glycine 13 and 14 of the coat protein.
  • the cysteine residues to be removed which are external of the ⁇ -hairpin are found at positions 46 and 101.
  • two mutants of the MS-2 coat protein may be obtained by standard commercially available techniques for site specific mutagenesis and the corresponding cDNA sequences introduced into standard expression vectors, which vectors are subjected to digestion with restriction enzymes to obtain separately the DNA fragment containing the mutated cys 46 site and the corresponding fragment containing the mutated cys 101 site, the fragments being subsequently ligated to give a doubly-mutated coat protein cDNA.
  • the doubly-mutated cDNA may then be subjected to site-directed mutagenesis using standard methods to introduce a cysteine residue in the ⁇ -hairpin region.
  • a desired molecular species there can be further linked to the introduced cysteine residue with or without interposition of a spacer moiety, a desired molecular species to be presented.
  • a desired molecular species are the epitopes described in copending Application No. 9201372.1, i.e. a 9-mer peptide sequence derived from the haemagglutinin of the human pathogen influenza virus (or a haemagglutinin moiety containing the epitope), or the IgE decapeptide described therein (which is also described and claimed in International Patent Application Publication No. W090/15878).
  • FMDV Foot and Mouth Disease Virus
  • LHRH luteinizing hormone releasing hormone
  • HIV gpl60 and 120 proteins enzymes such as horseradish peroxidase.
  • the invention also therefore extends to chimaeric proteins comprising such a foreign molecular species, and, optionally, a spacer, linked to a cysteine residue in the ⁇ -hairpin region of the coat protein. It will be appreciated that the foreign molecular species and optional spacer may be linked to the cysteine residue before or after capsid assembly.
  • the invention further extends to capsid assemblies of the chimaeric proteins of the invention. It has been found that such capsids can be expressed in £. coli as "phage empties" without the RNA of the live virus.
  • the generation of mixed capsid assemblies is contemplated, for example by prior disassembly of in vivo assembled samples followed by reassembly of a mixture.
  • Such capsid assemblies are intended, for example, to be capable of raising a mixed immune response and thus capable of finding application as vaccines for immunisation against a natural spectrum of viral epitopes in a population.
  • the invention therefore also extends to vaccines comprising one or more chimaeric proteins as defined above.
  • chimaeric proteins and assembled capsids of the present invention are envisaged for employment for example as vaccines as described in copending Application No. 9201372.1.
  • Such targeting techniques may be useful in the carrying of foreign moieties to and into specific cells.
  • Foreign moieties may be initially held by encapsidation within the coat protein capsids as described in our copending UK application No filed concurrently herewith.
  • Such foreign moieties may include genes, ⁇ ribozymes, anti-sense messages or cytotoxic agents.
  • the empty coat protein capsids can be readily expressed in comparatively high yield in E. col i .
  • the product is easily purified and it has been found that the assembled capsids show considerable stability with respect to a range of temperatures, pH and ionic strength.
  • the invention also allows one to present molecular species such as epitopes in a regular array on the surface of carrier capsids, at predetermined locations, which will maximise immune recognition, which relies on the multidentate nature of antibody molecules. The possibility also arises of maintaining the three dimensional structure of the epitope or other species being introduced.
  • the MS-2 system has considerable potential utility for the presentation of foreign molecular species on the surface of a spherical bacteriophage.
  • the system has a number of advantages over the filamentous bacteriophage alternatives.
  • the MS-2 coat protein is capable of facile self-assembly in the absence of nucleic acid unlike the filamentous phages in which assembly is concomitent with encapsidation of nucleic acid.
  • the filamentous coat protein must undergo a post translational processing and membrane insertion before assembly occurs whereas the MS-2 protein is unprocessed.
  • the MS-2 system also has the advantage of a detailed molecular model for the coat protein allowing the effects of foreign peptide insertion to be modelled.
  • Mutants were selected having either cysteine at position 46 substituted by serine or cysteine at position 101 substituted by serine.
  • Each single mutant DNA was expressed in E. coli to demonstrate the ability to self assemble.
  • the ser 46 single mutant from step A) was introduced into standard coat protein expression vector pTAC-CP and digested with SacI and X I restriction enzymes and the longer backbone fragment so obtained treated with calf intestinal phosphatase and then purified on agarose or polyacrylamide gels before electroelution and precipitation.
  • the ser 101 mutant from step A) was treated likewise with omission of the phosphatase treatment.
  • the smaller fragment containing the C-terminal portion of the coat protein gene was purified by gel electrophoresis.
  • the large fragment containing the mutated cys 46 site and the small fragment containing a mutated 101 site were ligated by standard methods.
  • the recombinant molecules thus obtained were used to transform JE. coli TGI to ampicillin-resistance and positive colonies checked for double mutation by DNA sequencing.
  • the doubly- utant ser 46/101 coat protein cDNA from step B was introduced into an M 13 sequencing vector by standard subcloning methods, a single stranded template for site-directed mutagenesis generated and a cysteine residue introduced at gly 14 using the commercially available site specific mutagenesis protocol based on nucleotide phosphothioates. There was thus obtained mutated cys 14 Ser 46/101 coat protein cDNA. D) Protein production and purification.
  • the isolated mutated cDNA was expressed in E. coli to confirm the capsid-forming ability of the recombinant protein.
  • the cys 114 ser 46/101 coat protein cDNA of C) above was introduced into the expression vector pTAC-CP and the resultant plasmid used to transform E. coli strain TGI to ampicillin resistance. Chimaeric proteins were expressed in accordance with the following procedure.
  • the resulting pellets were resuspended in 50mM Hepes, lOOmM NaCl, lOmM dithiothreitol (DTT), 5mM EDTA and ImM phenyl methyl sulphonyl fluoride (PMSF) and the cells lysed by sonication.
  • the cell lysate was then centrifuged at 15k, 20 mins, 4°C in a Beckman JA20 rotor and the supernatant passed down a NAP-25 column (Pharmacia) to change buffers to 20mM NaPi (sodium phosphate-based buffer) pH 7.0. 1ml fractions were collected from the NAP column, the MS-2 coat protein containing fractions (nos. 2 to 5 inclusive) added to an anti-MS-2 coat protein i munoaffinity column and the sample allowed to bind for 1 hour at room temperature with gentle agitation.
  • the column was washed with 20mM NaPi pH 7, then lO M NaPi/lOOmM NaCl pH 7.
  • the sample was eluted with 20mls 20mM acetic acid/200mM NaCl approx. pH 2.7 and the first 4mls collected.
  • Fig. 2 shows an electromicrograph of the immunoaffinity purified cys-14 modified capsids showing the presence of assembled particles similar to those produced by wild type coat proteins.
  • a halogen-activated galactose reagent was prepared as follows:
  • the mixture was left at room temperature for 15 mins after adding DTNB and then the OD ⁇ recorded.
  • Figure 4 shows the curve obtained when cysteine (4mM) was mixed with from 1 to lOmM activated galactose and left to stand at room temperature for 1 hour, followed by assay of lOO ⁇ l aliquots as described above using DTNB.
  • the cys 14 modified coat protein was reacted with DTNB as follows:
  • Purified cys 14 modified capsids were resuspended in buffer 1 containing ImM EDTA to a final concentration of 400 ⁇ g/ml . The following individual experiments were set up.
  • the purified cys 14 modified capsids were linked as described below to HA10, a 10-mer peptide sequence encompassing a nonapeptide epitope derived from the haemagglutinin of the human pathogen influenza virus and having an N-terminal cysteine residue extension, which 9-mer sequence YPYDVPDYA has been identified as containing one of the antigenic determinants by Wilson it al., Molecular and Cell Biology, May 1988, 2159-2165 and Cell, 37, 1984, 767-778.
  • the procedure involved an initial crosslinking step to form a disulphide linkage which was then oxidised.
  • test mixtures were prepared, in each case made up to 10 ⁇ l with water: 1) cys bridge + buffer 2 + ⁇ ME
  • the blots were washed with phosphate buffered saline (PBS) pH 7.6 containing Tween 20 (polyoxyethylene sorbitan monolaurate - 3ml per litre PBS) to equilibrate. They were then incubated for 1 hour at 37 ⁇ C with 35ml PBS-Tween plus 0.5% (w/v) bovine serum albumin (BSA), washed 6 x 5 min. with 200ml PBS-Tween and subsequently incubated overnight at 4°C with 35ml PBS-Tween +
  • PBS phosphate buffered saline
  • BSA bovine serum albumin
  • HRP horseradish peroxidase
  • HRP-maleimide conjugate Pierce Europe BV, Holland
  • cys 14 modified capsids and ⁇ ME were used to make the following mixtures, each of which was made up to lOO ⁇ l with lOOmM NaPi, pH 7.2: 1) 20 ⁇ g HRP-maleimide plus l ⁇ l ⁇ ME

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Abstract

A chimaeric protein is provided, capable of forming part of a capsid assembly and comprising the amino acid sequence of the coat protein of phage MS-2, or a conservatively modified variant thereof, or sufficient of said sequence or variant to retain the capability of forming a capsid assembly, which amino acid sequence has been modified by removal of the cysteine residues present externally of the N-terminal protuberant β-hairpin of the coat protein and insertion of a cysteine residue within the region corresponding to the N-terminal protuberant β-hairpin.

Description

CAPSID FORMING AND CYSTEIN MODIFIED CHIMAERIC MS2-C0AT PROTEIN
This invention relates to a chimaeric protein and is particularly directed to virus proteins containing foreign
5 inserts, the preparation of such proteins and their use, for example as vaccines or as carriers for presenting molecular species such as targeting moieties.
Copending GB Patent Application No. 9201372.1 describes the finding that epitope insertion in an identified class of virus
10 protein carriers can be specifically directed so that foreign epitopes are reliably presented on the surface of the protein capsid assembly after the expression of the chimaeric protein in a bacterial host.
It was by no means predictable that such a result could be
15 achieved. Not all viruses possess coat proteins which will self assemble : furthermore it is not possible to predict such self assembly. Even further, unlike animal viruses, bacterial viruses cannot naturally be expected to possess im unodominant regions and there is thus no guidance as to which region of a
20 bacterial virus coat protein (if any) could be modified in the reasonable expectation of inducing an immune response.
Accordingly, copending Application No. 9201372.1 relates to a chimaeric protein, capable of forming part of a capsid assembly and comprising the amino acid sequence of the coat
25 protein of phage MS-2, or a conservatively modified variant thereof, or sufficient of said sequence or variant to retain capsid-forming capability, which amino acid sequence has been modified by insertion of a foreign epitope in the region of the protein corresponding to an N-terminal β-hairpin, as determined
30 by X-ray crystallography of the whole phage particle.
Surprisingly, it was found that such a chimaeric protein can be expressed in a suitable bacterial host to yield capsids empty of the phage RNA and largely free of other nucleic acid contaminants. While it is contemplated that the insertion of a foreign epitope which is a linear polypeptide of comparatively short length, for example up to about 30 amino acid residues, can be accomplished, it will be appreciated that the presentation of non-linear epitopes by simple insertion in the β-hairpin may not be feasible. Thus many epitopes do not consist of simple linear fragments of polypeptide : instead they are made up of several such fragments spatially related by the overall three-dimensional folding of the native protein antigen, i.e. discontinuous epitopes. It is clearly desirable to be able to present such epitopes at the surface of the phage capsid.
It has now been found that the coat protein of MS-2 can be employed with a view to presenting a range of epitopes or other molecular species such as targeting moieties at the capsid surface by removing the cysteine residues which occur at sites not included within the N-terminal β-hairpin and introducing a cysteine residue within the β-hairpin region, which cysteine residue is then available for linking with desired antigens or other molecular species, suitably via an appropriate spacer. Therefore in accordance with the present invention there is provided a chimaeric protein capable of forming part of a capsid assembly and comprising the amino acid sequence of the coat protein of phage S-2, or a conservatively modified variant thereof, or sufficient of said sequence or variant to retain the capability of forming a capsid assembly, which amino acid sequence has been modified by removal of the cysteine residues present externally of the N-terminal protruberant β-hairpin of the coat protein and insertion of a cysteine residue within the region corresponding to the N-terminal protruberant β-hairpin. The cysteine residues external to the β-hairpin are suitably removed by mutation at the appropriate sites of the coat protein cDNA.
The inserted cysteine may be linked directly to a desired molecular species to be presented such as an epitope, especially a non-linear antigenie protein, or to other molecular species to be presented such as a targeting moiety. Alternatively, a spacer moiety may be employed between the cysteine and the molecular species to be presented, thus extending the range of species that can be presented.
Suitable spacer moieties are selected from known commercially available heterobifunctional crosslinking reagents which couple with the exposed cysteine thiol group. Examples of such cross-linkers are m-maleimidobenzoyl-N-hydroxy- sulfosuccini ide ester, N-succinimidyl-(4-iodoacetyl)amino- benzoate and N-succinimidyl-3-(2-pyridyldithio)propionate. The choice of crosslinker will depend on the molecular species to be presented and its size. Thus larger molecular species may require longer crosslinking moieties to minimize steric hindrance. The cross!inker may be linked first to the cysteine residue or first to the molecular species to be presented.
The chimaeric coat protein is preferably that derived from phage MS-2, but it may also be derived from related RNA-phages capable of replication in E. coli t such as phages R17, fr, GA, Qβ and SP. Such RNA-phages of physical structure similar to that of MS-2 will contain some chemical variation in the amino acid residues of the coat protein and are thus conservatively modified variants of MS-2 coat protein. While it is believed at present that substantially the entire coat protein may be required for capsid assembly, deletions and/or insertions of a relatively minor nature may also be possible whilst still retaining capsid-forming capability. Proteins having such modified sequences are included within the scope of the invention. As stated above, the foreign moiety is inserted at the region of the protein which in the assembled capsid corresponds to the N-terminal β-hairpin. The three-dimensional structure of the MS-2 phage particle has been published by Valegard e± al-, (Nature, 1990, 245, 36-41). The published data show that, firstly, the structure of the coat protein is not related to the eight-stranded β-barrel motif found in all other spherical RNA virus subunits whose structures are known at the present time. Secondly, although the coat protein exhibits quasi-equivalent inter-subunit contacts, there are no other devices, such as extended arms of polypeptide, helping to secure each protein conformer. The coat protein structure can be viewed in terms of three separate regions. These are not domains in the usual sense but could represent independent folding units. These regions are residues 1-20, which form the β-hairpin structure which protrudes from the surface of the phage forming the most distal radial feature. This region is followed by residues 21-94 which form five β-strands including the "FG-loop" which is the site of the only major confor ational change between quasi-equivalent conformers. These β-strands are then followed by two α-helices, residues 95-125, which interdigitate to secure dimers of the coat protein sub-units. Valegard et .a_]_. are concerned solely with the physical structure of the MS-2 virus and do not attempt to elucidate the mode of action of the virus. As explained above, the present invention comprises modification of the coat protein amino acid sequence by introduction of a cysteine residue in the region corresponding to the protruberant hairpin. The chimaeric protein of the invention has therefore been so modified in the region of amino acid residues 1 to 20, such numbering being with reference to the entire coat protein sequence of MS-2 as published by Fiers, Nature, 1976, 260, 500-507. Preferably the modification to introduce the cysteine residue is towards or at the middle of the hairpin region. It is preferred to introduce the cysteine in the region of the glycine 13 and 14 of the coat protein. The cysteine residues to be removed which are external of the β-hairpin are found at positions 46 and 101. They may be removed by any convenient conventional genetic engineering technique, suitably by site-specific utagenesis. In a preferred method of removing the unwanted cysteine residues, two mutants of the MS-2 coat protein, one singly mutated at cys 46 and one singly mutated at cys 101 may be obtained by standard commercially available techniques for site specific mutagenesis and the corresponding cDNA sequences introduced into standard expression vectors, which vectors are subjected to digestion with restriction enzymes to obtain separately the DNA fragment containing the mutated cys 46 site and the corresponding fragment containing the mutated cys 101 site, the fragments being subsequently ligated to give a doubly-mutated coat protein cDNA. The doubly-mutated cDNA may then be subjected to site-directed mutagenesis using standard methods to introduce a cysteine residue in the β-hairpin region.
There can be further linked to the introduced cysteine residue with or without interposition of a spacer moiety, a desired molecular species to be presented. Examples of such species are the epitopes described in copending Application No. 9201372.1, i.e. a 9-mer peptide sequence derived from the haemagglutinin of the human pathogen influenza virus (or a haemagglutinin moiety containing the epitope), or the IgE decapeptide described therein (which is also described and claimed in International Patent Application Publication No. W090/15878). Further alternative species which may be linked to the cysteine residue include Foot and Mouth Disease Virus (FMDV) VP1 , luteinizing hormone releasing hormone (LHRH) and HIV gpl60 and 120 proteins, and enzymes such as horseradish peroxidase.
The invention also therefore extends to chimaeric proteins comprising such a foreign molecular species, and, optionally, a spacer, linked to a cysteine residue in the β-hairpin region of the coat protein. It will be appreciated that the foreign molecular species and optional spacer may be linked to the cysteine residue before or after capsid assembly. The invention further extends to capsid assemblies of the chimaeric proteins of the invention. It has been found that such capsids can be expressed in £. coli as "phage empties" without the RNA of the live virus. The generation of mixed capsid assemblies is contemplated, for example by prior disassembly of in vivo assembled samples followed by reassembly of a mixture. Such capsid assemblies are intended, for example, to be capable of raising a mixed immune response and thus capable of finding application as vaccines for immunisation against a natural spectrum of viral epitopes in a population. The invention therefore also extends to vaccines comprising one or more chimaeric proteins as defined above.
The chimaeric proteins and assembled capsids of the present invention are envisaged for employment for example as vaccines as described in copending Application No. 9201372.1.
In addition, they are envisaged for employment for targeting, for example by addition of a galactose residue, which would direct the capsid to interact with specific cell surface receptors. Such targeting techniques may be useful in the carrying of foreign moieties to and into specific cells. Foreign moieties may be initially held by encapsidation within the coat protein capsids as described in our copending UK application No filed concurrently herewith. Such foreign moieties may include genes, ^ribozymes, anti-sense messages or cytotoxic agents.
It will be apparent that there are several advantages in using MS-2 and related phages as a presentation system. Thus the empty coat protein capsids can be readily expressed in comparatively high yield in E. col i . the product is easily purified and it has been found that the assembled capsids show considerable stability with respect to a range of temperatures, pH and ionic strength. While not wishing to be bound by any particular theory, it is postulated that the invention also allows one to present molecular species such as epitopes in a regular array on the surface of carrier capsids, at predetermined locations, which will maximise immune recognition, which relies on the multidentate nature of antibody molecules. The possibility also arises of maintaining the three dimensional structure of the epitope or other species being introduced.
It is believed that the MS-2 system has considerable potential utility for the presentation of foreign molecular species on the surface of a spherical bacteriophage. The system has a number of advantages over the filamentous bacteriophage alternatives. The MS-2 coat protein is capable of facile self-assembly in the absence of nucleic acid unlike the filamentous phages in which assembly is concomitent with encapsidation of nucleic acid. Furthermore, the filamentous coat protein must undergo a post translational processing and membrane insertion before assembly occurs whereas the MS-2 protein is unprocessed. The MS-2 system also has the advantage of a detailed molecular model for the coat protein allowing the effects of foreign peptide insertion to be modelled.
The following specific description illustrates the invention by way of example.
A) Isolation of single mutant MS-2 coat protein genes. Site directed mutagenesis and standard techniques were used to produce amino acid mutants at coat protein positions 46 and 101.
Mutants were selected having either cysteine at position 46 substituted by serine or cysteine at position 101 substituted by serine.
Each single mutant DNA was expressed in E. coli to demonstrate the ability to self assemble.
B) Preparation of doubly mutated cDNA.
The ser 46 single mutant from step A) was introduced into standard coat protein expression vector pTAC-CP and digested with SacI and X I restriction enzymes and the longer backbone fragment so obtained treated with calf intestinal phosphatase and then purified on agarose or polyacrylamide gels before electroelution and precipitation. The ser 101 mutant from step A) was treated likewise with omission of the phosphatase treatment. The smaller fragment containing the C-terminal portion of the coat protein gene was purified by gel electrophoresis. The large fragment containing the mutated cys 46 site and the small fragment containing a mutated 101 site were ligated by standard methods. The recombinant molecules thus obtained were used to transform JE. coli TGI to ampicillin-resistance and positive colonies checked for double mutation by DNA sequencing. C) Introduction of cysteine residue.
The doubly- utant ser 46/101 coat protein cDNA from step B was introduced into an M 13 sequencing vector by standard subcloning methods, a single stranded template for site-directed mutagenesis generated and a cysteine residue introduced at gly 14 using the commercially available site specific mutagenesis protocol based on nucleotide phosphothioates. There was thus obtained mutated cys 14 Ser 46/101 coat protein cDNA. D) Protein production and purification.
The isolated mutated cDNA was expressed in E. coli to confirm the capsid-forming ability of the recombinant protein. The cys 114 ser 46/101 coat protein cDNA of C) above was introduced into the expression vector pTAC-CP and the resultant plasmid used to transform E. coli strain TGI to ampicillin resistance. Chimaeric proteins were expressed in accordance with the following procedure.
5 x 5ml (2TY media with lOOμg/ml ampicillin) cultures of single colonies picked from transformation plates were grown for approx. 4hrs at 37°C and then used to inoculate 5 x 500ml flasks of 2TY plus ampicillin and the cultures were grown at 30°C. When the cultures reached ODg0o approx. 0.45 protein production was induced by adding ImM isopropyl-β-D-thio-galactoside (IPTG). Cells grown overnight were then centrifuged at 3k rpm, 30 mins, 4°C in a Beckman JA14 rotor. The resulting pellets were resuspended in 50mM Hepes, lOOmM NaCl, lOmM dithiothreitol (DTT), 5mM EDTA and ImM phenyl methyl sulphonyl fluoride (PMSF) and the cells lysed by sonication. The cell lysate was then centrifuged at 15k, 20 mins, 4°C in a Beckman JA20 rotor and the supernatant passed down a NAP-25 column (Pharmacia) to change buffers to 20mM NaPi (sodium phosphate-based buffer) pH 7.0. 1ml fractions were collected from the NAP column, the MS-2 coat protein containing fractions (nos. 2 to 5 inclusive) added to an anti-MS-2 coat protein i munoaffinity column and the sample allowed to bind for 1 hour at room temperature with gentle agitation.
The column was washed with 20mM NaPi pH 7, then lO M NaPi/lOOmM NaCl pH 7. The sample was eluted with 20mls 20mM acetic acid/200mM NaCl approx. pH 2.7 and the first 4mls collected.
The pH was immediately adjusted by titration with 1M
Tris.HCl pH 9 to pH 7-7.4 and the mixture centrifuged at 30k rpm, 4°C overnight (approx. 15 hrs) using a Beckman SW.55Ti rotor. The supernatant was decanted and the MS-2 protein pellet resuspended in a small volume of the required buffer.
Homogenous cys 14 modified capsids were obtained which were tested for their ability to react with an activated galactose reagent as described in E) below.
SDS-PAGE of the resultant immunoaffinity purified cys-14 modified capsids showed essentially a single component of the expected molecular weight. This result is shown in Fig. 1 where lane a) shows the cys-14 modified capsids, lanes b) and c) show wild type capsids respectively immunoaffinity purified and sucrose density purified and lane d) gives molecular weight standards.
Fig. 2 shows an electromicrograph of the immunoaffinity purified cys-14 modified capsids showing the presence of assembled particles similar to those produced by wild type coat proteins. E) Reaction of cys 14 modified protein with activated galactose.
In order to test the reactivity of the cys 14 modified MS-2 capsids, a halogen-activated galactose reagent was prepared as follows:
To a stirred solution of p-aminophenyl β-D-galactopyranoside (0.54g; 2 mmole) in water (4ml) and ethanol (6ml) was added iodoacetic anhydride (0.9g; 2.5 mmole) at room temperature. After 2 hours, the reaction was concentrated to dryness and the residue washed with ether (2 x 10ml). Crystallisation from ethanol gave the product as needles (0.7g; 80%), mp 158-160°C.
The reaction of the cys 14 modified capsids with the activated galactose was assayed using Ellman's reagent (dithio- nitrobenzoate, DTNB) which gives a characteristic absorption at OD412 on reaction with free -SH groups. Figure 3 shows a control curve for reaction of free cysteine with DTNB. The control curve was obtained using: lOOμl sample. lOOμl DTNB 4mg/ml in lOOmM Na2HP04 pH8 ("buffer 1"). 5ml "buffer 1".
The mixture was left at room temperature for 15 mins after adding DTNB and then the OD^ recorded.
Figure 4 shows the curve obtained when cysteine (4mM) was mixed with from 1 to lOmM activated galactose and left to stand at room temperature for 1 hour, followed by assay of lOOμl aliquots as described above using DTNB.
The cys 14 modified coat protein was reacted with DTNB as follows:
Purified cys 14 modified capsids were resuspended in buffer 1 containing ImM EDTA to a final concentration of 400μg/ml . The following individual experiments were set up.
1) lOOμl protein sample, lOOμl 5mM activated galactose.
2) lOOμl protein sample, 90μl buffer 1, 10μl 5mM activated galactose. 3) lOOμl protein sample, lOOμl buffer 1. Each was left stirring at room temperature for 1 hour before addition of 200μl DTNB 4mg/ml in ethanol to the stirring solution. 0D412 was recorded after 15 ins and the results are shown in Table 1 below. The number of free thiols decreased with increasing exposure to the galactose reagent, confirming that the cys 14 capsids had been derivatised with galactose.
Table 1
Sample O.D. 412nm Buffer blank 0.0
1) lOOμl MS2-cys; lOOμl gal 0.031
2) lOOμl MS2-cys; 90μl buffer; 10μl gal 0.080
3) lOOμl MS2-cys; lOOμl buffer 0.118 F) Linking of cys 14 modified coat protein to immunogenic peptide.
The purified cys 14 modified capsids were linked as described below to HA10, a 10-mer peptide sequence encompassing a nonapeptide epitope derived from the haemagglutinin of the human pathogen influenza virus and having an N-terminal cysteine residue extension, which 9-mer sequence YPYDVPDYA has been identified as containing one of the antigenic determinants by Wilson it al., Molecular and Cell Biology, May 1988, 2159-2165 and Cell, 37, 1984, 767-778. The procedure involved an initial crosslinking step to form a disulphide linkage which was then oxidised.
The following reagents were employed to make up four test reaction mixtures:
2μg cys 14 modified capsids (about 3μl ) ("cys bridge") lμl 1M Tris.HCl pH8, lOmM EDTA ("buffer 2") 17μg HA9 peptide (about 2μl) ("peptide") lμl 2-mercaptoethanol ("βME")
The following four test mixtures were prepared, in each case made up to 10μl with water: 1) cys bridge + buffer 2 + βME
2) cys bridge + buffer 2
3) cys bridge + buffer 2 + βME + peptide
4) cys bridge + buffer 2 + peptide The mixtures were incubated for 1 hour at room temperature. There was then added lμl of a mixture of 0.37M sodium tetrathionate and 1.6M sodium sulphite (which had been freshly prepared in accordance with the method of Morehead gt a].., Biochem., 21, 1984, 2500). The mixtures were left overnight at room temperature.
The mixtures were analysed using a PAGE Schagger System (Schagger e± al_., 1987, Anal. Biochem., Ki£, 368-379), blotted onto nitrocellulose paper using a Bio-Rad Western blotting apparatus, with a transfer buffer of 39mM glycine, 48mM Tris, 0.1% (w/v) sodium dodecyl sulphate (SDS) and 20% methanol for a transfer time of 1 hour at 450mA.
The blots were washed with phosphate buffered saline (PBS) pH 7.6 containing Tween 20 (polyoxyethylene sorbitan monolaurate - 3ml per litre PBS) to equilibrate. They were then incubated for 1 hour at 37βC with 35ml PBS-Tween plus 0.5% (w/v) bovine serum albumin (BSA), washed 6 x 5 min. with 200ml PBS-Tween and subsequently incubated overnight at 4°C with 35ml PBS-Tween +
0.5% (w/v) BSA together with lOOμl mouse anti-HA9 monoclonal antibody (obtained from Balcore Co., Berkley, USA). There then followed washing with PBS-Tween (6 x 5 min. - 200ml) and incubation for half an hour with 35ml PBS-Tween + 0.5% (w/v) BSA together with 50μl goat anti-mouse IgG horseradish peroxidase
(HRP) conjugate. After further washing (6 x 6 min. - 200ml PBS-Tween), the gel was excited by luminol Western blotting reagents (Amersham) and visualised.
The results showed that only a single band in the lane corresponding to sample number 4 cross-reacted with the anti-HA9 antibody. This is the expected result, samples 1-3 being negative controls. Thus it is possible to couple linear peptide fragments to cys 14 capsids using these methods. G) Covalent cross-linking of cys 14 modified coat protein to an enzyme.
The purified cys 14 modified capsids described in E) above were covalently linked via a maleimide group to the enzyme horseradish peroxidase (HRP) as follows:
HRP-maleimide conjugate (Pierce Europe BV, Holland), cys 14 modified capsids and βME were used to make the following mixtures, each of which was made up to lOOμl with lOOmM NaPi, pH 7.2: 1) 20μg HRP-maleimide plus lμl βME
2) 20μg cys 14 modified capsids plus Iμl βME
3) 20μg cys 14 modified capsids plus 20μg HRP-maleimide Sample 3) was left for 1 hour at room temperature and then βME added to quench any remaining thiols. Samples 1-3 were then fractionated by HPLC gel filtration chromatography on PW 3000, 2 x 30cm columns, in lOOmM NaPi, pH 7.2 at a flow rate of 0.5ml/min. Fractions (1 min. - 0.5ml) of the eluate were than assayed for HRP activity using the commercially available kit (ABTS reagent, Pierce), enzyme activity being estimated by observing the increased absorbance of solutions at 410nm. The data showed a significant increase over background levels in fractions corresponding to the 0D2gø peak of cys 14 assembled material of sample 3.

Claims

1. A chimaeric protein capable of forming part of a capsid assembly and comprising the amino acid sequence of the coat protein of phage MS-2, or a conservatively modified variant thereof, or sufficient of said sequence or variant to retain the capability of forming a capsid assembly, which amino acid sequence has been modified by removal of the cysteine residues present externally of the N-terminal protruberant β-hairpin of the coat protein and insertion of a cysteine residue within the region corresponding to the N-terminal protruberant β-hairpin.
2. A chimaeric protein according to claim 1 wherein the cysteine residue has been inserted in the region of the glycine 13 and 14 residues of the coat protein.
3. A chimaeric protein according to claim 2 wherein the cysteine residue has been inserted to replace glycine 14.
4. A chimaeric protein according to claim 1, 2 or 3 wherein the cysteines externally of the β hairpin have been replaced by serine.
5. A chimaeric protein according to claim 1, 2, 3 or 4 wherein the inserted cysteine residue has been linked directly or indirectly to a foreign molecular species.
6. A chimaeric protein according to claim 5 wherein the inserted cysteine residue has been linked to the foreign molecular species via a spacer moiety.
7. A chimaeric protein according to claim 6 wherein the spacer moiety is derived from a bifunctional crosslinking reagent selected from m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester, N-succinimidyl-(4-iodoacetyl)aminobenzoate and N-succinimidyl-3- (2-pyridyldithio)propionate.
8. A chimaeric protein according to any one of claims 1 to 7 wherein the cysteine residue has been linked directly or indirectly to an antigenie protein.
9. A chimaeric protein according to any one of claims 1 to 7 wherein the cysteine residue has been linked directly or indirectly to a targeting moiety.
10. A chimaeric protein according to claim 9 wherein the targeting moiety comprises galactose.
11. Capsids comprising the chimaeric protein of any one of the preceding claims or a mixture of such proteins.
12. A method of preparing a chimaeric protein according to any one of the preceding claims comprising preparing two single mutants of the phage coat protein, one mutated to remove the cysteine 46 residue and one mutated to remove the cysteine 101 residue, digesting and religating the corresponding cDNAs to provide a doubly-mutated coat protein cDNA and subjecting the doubly- mutated cDNA to site-directed mutagenesis to introduce a cysteine residue within the region corresponding to the N-terminal protruberant β-hairpin and expressing the resulting cDNA to obtain the chimaeric protein.
13. A method according to claim 12 wherein the expression is carried out in E. coli .
14. An expression vector comprising DNA coding for the chimaeric protein of any one of claims 1 to 4.
15. A vaccine preparation comprising a protein according to claim 8 or capsids comprising such a protein.
PCT/GB1992/001159 1991-06-28 1992-06-26 Capsid forming and cystein modified chimaeric ms2-coat protein WO1993000434A1 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
DE69232069T DE69232069T2 (en) 1991-06-28 1992-06-26 CAPSID PRODUCING AND CYSTEIN-MODIFIED CHIMERIC MS-2-ENVELOPE PROTEIN
AU21955/92A AU657560B2 (en) 1991-06-28 1992-06-26 Capsid forming and cystein modified chimaeric MS2-coat protein
US08/167,982 US5698424A (en) 1991-06-28 1992-06-26 Capsid forming and cystein modified chimaeric MS2-coat protein
EP92913894A EP0591369B1 (en) 1991-06-28 1992-06-26 Capsid forming and cystein modified chimaeric ms2-coat protein
AT92913894T ATE205880T1 (en) 1991-06-28 1992-06-26 CAPSID PRODUCING AND CYSTEIN MODIFIED CHIMERIC MS-2 ENVELOPE PROTEIN
JP50123093A JP3514755B2 (en) 1991-06-28 1992-06-26 Capsid-forming cysteine-modified MS2-coat chimeric protein
KR1019930703986A KR100222163B1 (en) 1991-06-28 1992-06-26 Capsid forming and cystein modified chimeric ms2-coat protein
FI935877A FI110613B (en) 1991-06-28 1993-12-27 The capsid-forming and cysteine-modified chimeric MS2 envelope protein
NO19934842A NO315050B1 (en) 1991-06-28 1993-12-27 Capsid-forming and cysteine-modified chimeric MS2 coat protein

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GB919114003A GB9114003D0 (en) 1991-06-28 1991-06-28 Chimaeric protein
GB9114003.8 1991-06-28

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