WO1990006953A3 - Method for preparing water soluble polypeptides - Google Patents

Method for preparing water soluble polypeptides Download PDF

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Publication number
WO1990006953A3
WO1990006953A3 PCT/US1989/005743 US8905743W WO9006953A3 WO 1990006953 A3 WO1990006953 A3 WO 1990006953A3 US 8905743 W US8905743 W US 8905743W WO 9006953 A3 WO9006953 A3 WO 9006953A3
Authority
WO
WIPO (PCT)
Prior art keywords
receptors
water soluble
preparing water
soluble polypeptides
heteromultimer
Prior art date
Application number
PCT/US1989/005743
Other languages
French (fr)
Other versions
WO1990006953A2 (en
Inventor
Sarah C Bodary
Cornelia M Gorman
John W Mclean
Mary A Napier
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Priority to DE68929402T priority Critical patent/DE68929402T2/en
Priority to AT90901448T priority patent/ATE217887T1/en
Priority to EP90901448A priority patent/EP0452364B1/en
Priority to JP50189990A priority patent/JP3608572B2/en
Publication of WO1990006953A2 publication Critical patent/WO1990006953A2/en
Publication of WO1990006953A3 publication Critical patent/WO1990006953A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70546—Integrin superfamily
    • C07K14/70553—Integrin beta2-subunit-containing molecules, e.g. CD11, CD18
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70546—Integrin superfamily
    • C07K14/70557—Integrin beta3-subunit-containing molecules, e.g. CD41, CD51, CD61
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/32—Fusion polypeptide fusions with soluble part of a cell surface receptor, "decoy receptors"
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Methods are provided for the preparation in recombinant host cells of biologically active soluble variants of discretely encoded, heteromultimer polypeptide receptors. Such variants are synthesized by the secretion from recombinant transformants of transmembrane-modified heteromultimer receptors. Preferred receptors are extracellular matrix, cell surface, or plasma protein-binding receptors such as GPIIb-IIIa.
PCT/US1989/005743 1988-12-22 1989-12-20 Method for preparing water soluble polypeptides WO1990006953A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DE68929402T DE68929402T2 (en) 1988-12-22 1989-12-20 METHOD FOR PRODUCING WATER-SOLUBLE POLYPEPTIDES
AT90901448T ATE217887T1 (en) 1988-12-22 1989-12-20 METHOD FOR PRODUCING WATER SOLUBLE POLYPEPTIDES
EP90901448A EP0452364B1 (en) 1988-12-22 1989-12-20 Method for preparing water soluble polypeptides
JP50189990A JP3608572B2 (en) 1988-12-22 1989-12-20 Method for producing water-soluble polypeptide

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US29022488A 1988-12-22 1988-12-22
US44449089A 1989-12-01 1989-12-01
US290,224 1989-12-01
US444,490 1989-12-01

Publications (2)

Publication Number Publication Date
WO1990006953A2 WO1990006953A2 (en) 1990-06-28
WO1990006953A3 true WO1990006953A3 (en) 1990-08-09

Family

ID=26966065

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1989/005743 WO1990006953A2 (en) 1988-12-22 1989-12-20 Method for preparing water soluble polypeptides

Country Status (8)

Country Link
US (3) US5726037A (en)
EP (3) EP1201757A3 (en)
JP (2) JP3608572B2 (en)
AT (1) ATE217887T1 (en)
AU (1) AU638964B2 (en)
DE (1) DE68929402T2 (en)
ES (1) ES2176174T3 (en)
WO (1) WO1990006953A2 (en)

Families Citing this family (42)

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Publication number Priority date Publication date Assignee Title
US6018026A (en) * 1988-01-22 2000-01-25 Zymogenetics, Inc. Biologically active dimerized and multimerized polypeptide fusions
US5750375A (en) * 1988-01-22 1998-05-12 Zymogenetics, Inc. Methods of producing secreted receptor analogs and biologically active dimerized polypeptide fusions
US5567584A (en) * 1988-01-22 1996-10-22 Zymogenetics, Inc. Methods of using biologically active dimerized polypeptide fusions to detect PDGF
US5116964A (en) 1989-02-23 1992-05-26 Genentech, Inc. Hybrid immunoglobulins
US5891841A (en) * 1991-06-11 1999-04-06 The Center For Blood Research, Inc. Methods of using intercellular adhesion molecule-3 (ICAM-3), antibodies thereto, and soluble fragments thereof
AU2254792A (en) * 1991-07-01 1993-02-11 Blood Center Of Southeastern Wisconsin, The Pen polymorphism of human platelet membrane glycoprotein iiia and diagnostic and therapeutic application thereof
US5939524A (en) * 1991-12-09 1999-08-17 The Scripps Research Institute Platelet GPIII P1A1 and P1A2 epitopes, their preparation and use
US6100383A (en) * 1992-01-27 2000-08-08 Icos Corporation Fusion proteins comprising ICAM-R polypeptides and immunoglobulin constant regions
JP3616090B2 (en) * 1992-01-27 2005-02-02 イコス コーポレイション Intercellular adhesion molecule-related protein
US5811517A (en) * 1992-01-27 1998-09-22 Icos Corporation ICAM-related protein variants
US5989843A (en) * 1992-01-27 1999-11-23 Icos Corporation Methods for identifying modulators of protein kinase C phosphorylation of ICAM-related protein
US6153395A (en) * 1992-01-27 2000-11-28 Icos Corporation ICAM-related protein
US6818743B1 (en) 1992-01-27 2004-11-16 Icos Corporation I-CAM related protein
US5532127A (en) * 1992-01-27 1996-07-02 Icos Corporation Assay for 1-CAM related protein expression
US5525487A (en) * 1992-01-27 1996-06-11 Icos Corporation DNA encoding I-CAM related protein
US5837822A (en) * 1992-01-27 1998-11-17 Icos Corporation Humanized antibodies specific for ICAM related protein
FR2726576B1 (en) * 1994-11-07 1997-01-31 Pf Medicament PRODUCTION OF HYDROPHOBIC PEPTIDE-LIKE PEPTIDES, RECOMBINANT PEPTIDE, CORRESPONDING DNA SEQUENCE
DE69732711T2 (en) * 1996-07-12 2006-03-16 Genentech, Inc., South San Francisco GAMMA-heregulin
DE69740038D1 (en) * 1996-07-12 2010-12-16 Genentech Inc
DE69739295D1 (en) * 1996-12-09 2009-04-23 Merck Patent Gmbh Soluble, recombinant alphaV beta3 adhesion receptor
US7122636B1 (en) * 1997-02-21 2006-10-17 Genentech, Inc. Antibody fragment-polymer conjugates and uses of same
SI2319928T1 (en) 1998-10-23 2013-08-30 Kirin-Amgen, Inc. Dimeric thrombopoietin peptide mimetics binding to MP1 receptor and having thrombopoietic activity
US7488590B2 (en) * 1998-10-23 2009-02-10 Amgen Inc. Modified peptides as therapeutic agents
US6660843B1 (en) * 1998-10-23 2003-12-09 Amgen Inc. Modified peptides as therapeutic agents
US20030055231A1 (en) * 1998-10-28 2003-03-20 Jian Ni 12 human secreted proteins
US6867203B2 (en) 1998-12-29 2005-03-15 Abbott Laboratories Cell adhesion-inhibiting antiinflammatory and immune-suppressive compounds
SE9902056D0 (en) * 1999-06-03 1999-06-03 Active Biotech Ab An integrin heterodimer and an alpha subunit thereof
CA2407956A1 (en) * 2000-05-03 2001-11-08 Amgen Inc. Modified peptides as therapeutic agents
US7456261B2 (en) * 2000-08-22 2008-11-25 Genentech, Inc. PRO9830 antibodies
US7332168B2 (en) * 2000-08-22 2008-02-19 Micromet Ag Composition for the elimination of autoreactive B-cells
ATE384783T1 (en) 2001-10-19 2008-02-15 Zymogenetics Inc DIMERIZED GROWTH FACTOR AND MATERIALS AND METHODS FOR PRODUCING IT
FR2856407B1 (en) * 2003-06-19 2007-10-12 Inst Nat Sante Rech Med PROCESS FOR PRODUCING A RECOMBINANT PROTEIN OF INTEREST AND PROTEIN PRODUCED
CA2578046A1 (en) 2004-09-07 2006-03-16 Archemix Corp. Aptamer medicinal chemistry
CA2579374A1 (en) 2004-09-07 2006-03-30 Archemix Corp. Aptamers to von willebrand factor and their use as thrombotic disease therapeutics
US7566701B2 (en) 2004-09-07 2009-07-28 Archemix Corp. Aptamers to von Willebrand Factor and their use as thrombotic disease therapeutics
ES2629397T3 (en) * 2004-09-24 2017-08-09 Amgen Inc. Modified Fc molecules
US8008453B2 (en) 2005-08-12 2011-08-30 Amgen Inc. Modified Fc molecules
WO2007057665A2 (en) * 2005-11-15 2007-05-24 Cambridge Enterprise Limited Detection of human platelet antigen (hpa) antibodies
CA2878344A1 (en) 2012-07-06 2014-01-09 Novartis Ag Complexes of cytomegalovirus proteins
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0244267A2 (en) * 1986-05-02 1987-11-04 Genentech, Inc. Variants of decay accelerating factor (DAF) prepared by recombinant DNA technology
WO1989000200A1 (en) * 1987-07-08 1989-01-12 Scripps Clinic And Research Foundation Peptides and antibodies that inhibit platelet adhesion

Family Cites Families (7)

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NZ209307A (en) * 1983-08-30 1990-07-26 Genentech Inc Diagnostic product: antigenic peptide produced by recombinant dna techniques
US5498600A (en) * 1984-10-12 1996-03-12 Zymogenetics, Inc. Biologically active mosaic proteins
US4761371A (en) * 1985-02-12 1988-08-02 Genentech, Inc. Insulin receptor
US4859609A (en) * 1986-04-30 1989-08-22 Genentech, Inc. Novel receptors for efficient determination of ligands and their antagonists or agonists
IE81149B1 (en) * 1987-02-12 2000-05-03 Genentech Inc Methods and deoxyribonucleic acid for the preparation of tissue factor protein
EP0303692A4 (en) * 1987-02-26 1990-12-05 Dana Farber Cancer Institute Cloning of lfa-1
WO1991019511A1 (en) * 1990-06-18 1991-12-26 The General Hospital Corporation CONTROLLING CELLULAR IMMUNE/INFLAMMATORY RESPONSES WITH β2 INTEGRINS

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0244267A2 (en) * 1986-05-02 1987-11-04 Genentech, Inc. Variants of decay accelerating factor (DAF) prepared by recombinant DNA technology
WO1989000200A1 (en) * 1987-07-08 1989-01-12 Scripps Clinic And Research Foundation Peptides and antibodies that inhibit platelet adhesion

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Annual Reviews of Cell Biology, Vol. 3, 1987, Annual Reviews Inc., C.A. BUCK: "Cell surface Receptors for Extracellurlar Matrix Molecules", see pages 179-205 *
Proc. Natl. Acad. Sci. USA, Vol. 85, November 1988, (Washington, D.C., US), P.F. BRAY et al.: "Physical Linkage of the Genes for Platelet Membrane Glycoproteins IIb and IIIa", pages 8683-8687 *
The Journal of Biological Chemistry, Vol. 264, No. 32, 15 November 1989, The American Scoiety for Biochemistry and Molecular Biology, Inc., (US), S.C. BODARY et al.: "Expression of Recombinant Platelet Glycoprotein IIBIIIA Results in a Functional Fibrinogen-Binding Complex", see pages 18859-18862 *

Also Published As

Publication number Publication date
EP0452364A1 (en) 1991-10-23
JP3608572B2 (en) 2005-01-12
US5726290A (en) 1998-03-10
AU4832690A (en) 1990-07-10
ES2176174T3 (en) 2002-12-01
EP1201756A3 (en) 2002-10-30
AU638964B2 (en) 1993-07-15
DE68929402D1 (en) 2002-06-27
DE68929402T2 (en) 2002-12-05
US5837486A (en) 1998-11-17
EP0452364B1 (en) 2002-05-22
EP1201757A3 (en) 2002-09-11
ATE217887T1 (en) 2002-06-15
JPH04502327A (en) 1992-04-23
WO1990006953A2 (en) 1990-06-28
EP1201756A2 (en) 2002-05-02
US5726037A (en) 1998-03-10
EP1201757A2 (en) 2002-05-02
JP2002112796A (en) 2002-04-16

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