WO1990000350A1 - Cryoprotectant composition - Google Patents
Cryoprotectant composition Download PDFInfo
- Publication number
- WO1990000350A1 WO1990000350A1 PCT/US1989/002818 US8902818W WO9000350A1 WO 1990000350 A1 WO1990000350 A1 WO 1990000350A1 US 8902818 W US8902818 W US 8902818W WO 9000350 A1 WO9000350 A1 WO 9000350A1
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- WIPO (PCT)
- Prior art keywords
- hours
- plant
- cryoprotectant
- thfa
- survived
- Prior art date
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 42
- 239000002577 cryoprotective agent Substances 0.000 title claims abstract description 37
- BSYVTEYKTMYBMK-UHFFFAOYSA-N tetrahydrofurfuryl alcohol Chemical compound OCC1CCCO1 BSYVTEYKTMYBMK-UHFFFAOYSA-N 0.000 claims abstract description 68
- 238000007710 freezing Methods 0.000 claims abstract description 46
- 230000008014 freezing Effects 0.000 claims abstract description 46
- 238000000034 method Methods 0.000 claims abstract description 22
- 239000001089 [(2R)-oxolan-2-yl]methanol Substances 0.000 claims abstract description 19
- 239000007864 aqueous solution Substances 0.000 claims abstract description 18
- YNOGYQAEJGADFJ-UHFFFAOYSA-N oxolan-2-ylmethanamine Chemical compound NCC1CCCO1 YNOGYQAEJGADFJ-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000000243 solution Substances 0.000 claims description 16
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 14
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 14
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 14
- 239000002736 nonionic surfactant Substances 0.000 claims description 10
- -1 polyethylene Polymers 0.000 claims description 8
- ZORQXIQZAOLNGE-UHFFFAOYSA-N 1,1-difluorocyclohexane Chemical compound FC1(F)CCCCC1 ZORQXIQZAOLNGE-UHFFFAOYSA-N 0.000 claims 2
- 239000004698 Polyethylene Substances 0.000 claims 2
- 229920000573 polyethylene Polymers 0.000 claims 2
- 235000011069 sorbitan monooleate Nutrition 0.000 claims 2
- 239000001593 sorbitan monooleate Substances 0.000 claims 2
- 229940035049 sorbitan monooleate Drugs 0.000 claims 2
- 241000196324 Embryophyta Species 0.000 description 74
- 239000003795 chemical substances by application Substances 0.000 description 42
- 238000012360 testing method Methods 0.000 description 26
- XPFVYQJUAUNWIW-UHFFFAOYSA-N furfuryl alcohol Chemical compound OCC1=CC=CO1 XPFVYQJUAUNWIW-UHFFFAOYSA-N 0.000 description 21
- 241001495448 Impatiens <genus> Species 0.000 description 15
- 229930190166 impatien Natural products 0.000 description 15
- 229920001223 polyethylene glycol Polymers 0.000 description 15
- 230000003750 conditioning effect Effects 0.000 description 14
- 238000010521 absorption reaction Methods 0.000 description 13
- 238000011156 evaluation Methods 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 241000720913 Fuchsia Species 0.000 description 11
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 11
- 239000004202 carbamide Substances 0.000 description 11
- 230000004083 survival effect Effects 0.000 description 10
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- DNIAPMSPPWPWGF-UHFFFAOYSA-N monopropylene glycol Natural products CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 6
- 235000013772 propylene glycol Nutrition 0.000 description 6
- 239000004094 surface-active agent Substances 0.000 description 6
- 239000003570 air Substances 0.000 description 5
- 235000021508 Coleus Nutrition 0.000 description 4
- 244000061182 Coleus blumei Species 0.000 description 4
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 4
- 229920000053 polysorbate 80 Polymers 0.000 description 4
- 238000005507 spraying Methods 0.000 description 4
- 235000002566 Capsicum Nutrition 0.000 description 3
- 241000758706 Piperaceae Species 0.000 description 3
- 230000001143 conditioned effect Effects 0.000 description 3
- DDRPCXLAQZKBJP-UHFFFAOYSA-N furfurylamine Chemical compound NCC1=CC=CO1 DDRPCXLAQZKBJP-UHFFFAOYSA-N 0.000 description 3
- 231100000252 nontoxic Toxicity 0.000 description 3
- 230000003000 nontoxic effect Effects 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- 101100536354 Drosophila melanogaster tant gene Proteins 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 2
- 239000002202 Polyethylene glycol Substances 0.000 description 2
- 240000003768 Solanum lycopersicum Species 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 description 2
- 229920001296 polysiloxane Polymers 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 0 *C1OCCC1 Chemical compound *C1OCCC1 0.000 description 1
- XZIIFPSPUDAGJM-UHFFFAOYSA-N 6-chloro-2-n,2-n-diethylpyrimidine-2,4-diamine Chemical compound CCN(CC)C1=NC(N)=CC(Cl)=N1 XZIIFPSPUDAGJM-UHFFFAOYSA-N 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 235000008098 Oxalis acetosella Nutrition 0.000 description 1
- 241000722363 Piper Species 0.000 description 1
- 244000126309 Trifolium dubium Species 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 239000012080 ambient air Substances 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 150000004292 cyclic ethers Chemical group 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229910003460 diamond Inorganic materials 0.000 description 1
- 239000010432 diamond Substances 0.000 description 1
- 230000003292 diminished effect Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- KPUWHANPEXNPJT-UHFFFAOYSA-N disiloxane Chemical class [SiH3]O[SiH3] KPUWHANPEXNPJT-UHFFFAOYSA-N 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 150000002170 ethers Chemical class 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 125000002425 furfuryl group Chemical group C(C1=CC=CO1)* 0.000 description 1
- 238000007496 glass forming Methods 0.000 description 1
- 239000003630 growth substance Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 238000005984 hydrogenation reaction Methods 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 229920001451 polypropylene glycol Polymers 0.000 description 1
- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 150000003138 primary alcohols Chemical class 0.000 description 1
- 150000003141 primary amines Chemical class 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 229940035044 sorbitan monolaurate Drugs 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/08—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings with oxygen as the ring hetero atom
Definitions
- This invention relates to plant cryoprotectant compositions and methods for preventing damage to plants caused by exposure to freezing temperatures for limited periods.
- Freezing temperatures are a major cause of damage to plants and plant tissue. According to one estimate, approximately $1.5 billion of agricultural products are annually lost to freezing temperatures in the United States. In Florida alone, more than $3 billion of frost- damage to citrus trees has been reported over the last five years.
- a plant cryoprotectant composition that increases the hardiness of plants and plant tissue.
- Another object of this invention is the provision of a plant cryoprotectant composition which is relatively inexpensive, non-toxic, and environmentally acceptable.
- a further object of this invention is the provision of a method for the cryoprotection of plants and plant tissue.
- the present invention is directed to an aqueous plant and plant tissue cryoprotec ⁇ tant solution containing between about 0.005 and about 25 wt.% of a cryoprotectant component selected from the group consisting of tetrahydrofurfuryl alcohol, tetrahydro ⁇ furfuryl amine and mixtures thereof.
- a cryoprotectant component selected from the group consisting of tetrahydrofurfuryl alcohol, tetrahydro ⁇ furfuryl amine and mixtures thereof.
- the invention is further directed to a method of increasing the resistance of plant tissue to damage caused by freezing conditions comprising applying to the plant tissue surfaces at an ambient non-freezing temperature, an aqueous solution containing between about 0.005 and about 25 wt.% of a cryoprotectant component selected from the group consisting of tetrahydrofurfuryl alcohol, tetrahydro ⁇ furfuryl amine and mixtures thereof at least about 4 hours prior to the onset of such freezing conditions.
- a cryoprotectant component selected from the group consisting of tetrahydrofurfuryl alcohol, tetrahydro ⁇ furfuryl amine and mixtures thereof at least about 4 hours prior to the onset of such freezing conditions.
- a plant cryoprotectant composition which comprises an aqueous solution containing a cryoprotectant component selected from the group consisting of tetrahydro ⁇ furfuryl alcohol, tetrahydrofurfuryl amine or mixtures thereof.
- a cryoprotectant component selected from the group consisting of tetrahydro ⁇ furfuryl alcohol, tetrahydrofurfuryl amine or mixtures thereof.
- the composition comprises between about 0.005 and about 25 wt% of the cryoprotectant component and most preferably comprises between about 0.1 and about 5 wt% of the cryoprotectant component.
- Tetrahydrofurfuryl alcohol is a colorless, high boiling, primary alcohol having the following structure:
- Tetrahydrofurfuryl amine is a colorless, high boiling primary amine having the following structure:
- tetrahydrofurfuryl alcohol is preferred in accordance with the present invention.
- Tetrahydrofurfuryl alcohol is produced by hydrogenation of furfuryl alcohol and is marketed under the name THFA.
- THFA tetrahydrofur uryl alcohol
- tetrahydrofur uryl alcohol exhibits behavior characteristics of both alcohols and ethers. Due to its cyclic ether structure, tetrahydrofurfuryl alcohol possesses distinctly unique solvent properties which are desirable for cryoprotecting agents, such as:
- cryoprotectant composition of this invention The resistance of plants and plant tissue to frost and low temperatures, including subfreezing temperatures, are increased for limited periods through the application of the cryoprotectant composition of this invention by spraying or dipping.
- any suitable plant spray apparatus suitable for spraying aqueous solutions may be employed.
- the plants to be treated are thoroughly sprayed so that all of the plant tissue surfaces are completely covered. Due to the size or shape of a plant, a single application may require two or more sprayings.
- the cryoprotectant composition is applied at ambient, non-freezing temperatures, i.e., the temperature of the air surrounding the plant or plant tissue is above 32 ⁇ F.
- the cryoprotectant composition is applied when the ambient air temperature is at least 35°F. (5)
- the cryoprotectant composition may be formulated and supplied to the user in concentrated form and diluted to the desired strength prior to application to the plant. No special handling or mixing steps are required. THFA and tetrahydrofurfuryl amine are stable in aqueous solution.
- compositions are stable to light and do not need to be stored in an opaque container nor prepared immediately prior to application.
- cryoprotectant composition include a non-ionic surfactant.
- a non-ionic surfactant Two different surfactants, polyoxy ⁇ ethylene sorbitan monolaurate (Tween 20) and polyoxyethylene sorbitan monooleate (Tween 80) have been found to improve the wetability of the composition and thereby improve its effectiveness.
- non-ionic surfactants it is preferred that the cryoprotectant composition contain between about 0.05 and about 0.5 wt.% non-ionic surfactant.
- cryoprotectant composi ⁇ tion The e fectiveness of the cryoprotectant composi ⁇ tion is not diminished by the inclusion of such compounds as organic/inorganic fertilizers, pesticides, plant hormones, growth regulators, other polymers, and coating materials.
- the composition is compatible with urea; the introduction of urea via a 1:1 THFA:UREA mixture, did not affect the wet ⁇ ability or the effectiveness of the agent. Nevertheless, it is presently preferred that the cryoprotectant composi ⁇ tion have an essential absence of pesticides.
- the cryoprotectant composition of this invention may be applied to the plants immediately prior to exposure to freezing conditions, it is preferred that the composition be applied between about 4 hours and 1 week prior thereto.
- the cryoprotectant composition be applied twice prior to the onset of freezing temperatures, the first application being made between about several days and about one week prior to the onset of freezing temperatures and ' constituting a conditioning application.
- the second appli ⁇ cation is then preferably made a sufficient period prior to the onset of freezing temperatures to permit absorption of the composition, e.g., at least about 4 hours.
- the pots holding the conditioned plants were fitted into a polyurethane insulated box having circular openings in the top. Each opening was sized to snugly receive a single pot holding a conditioned plant and the box had sufficient depth so that substantially the entire pot was contained within the insulated box while the condi ⁇ tioned plant stood outside the box.
- the box was heated by circulation of room temperature air through its interior.
- the insulated box holding the plants were placed inside a commercial freezer that was modified to achieve dynamic temperature control to within 0.01°C.
- the air temperature inside the freezer was monitored on a CRT screen and also recorded on a chart recorder. The air temperature inside the freezer was gradually reduced (4°C/hr) from an initial temperature (10°C) until the set point temperature was achieved.
- the set temperature was maintained for a period of three or four hours and then gradually warmed (4°C/hr) to a final temperature (i.5°C) .
- a final temperature i.5°C
- the maximum time for exposure to freezing temperatures was limited to 6 hours because beyond -that, the pots could not be kept from freezing. Instead, the tests in which plants survived exposure for 6 hours were repeated a few times to determine whether the plants could survive longer exposure, like those encountered under actual conditions in the field.
- the antifrost agents evaluated were the following:
- a non-ionic surfactant GOLDEN FROST FREE Plant Products Corporation; Vero Beach Florida
- a polyoxyethylenated polyoxypropylene copolymer obtained from Diamond Shamrock Corporation under the trademark Monolan 4500, dissolved in Dl water to make aqueous 0.3% and 0.4% solutions designated PPC707
- PPC707 a polyoxyethylenated polyoxypropylene copolymer obtained from Diamond Shamrock Corporation under the trademark Monolan 4500, dissolved in Dl water to make aqueous 0.3% and 0.4% solutions designated PPC707
- PS/PEG, and 5% THFA are effective in protecting fuchsias from the ravages of frost.
- Freezing survival tests for fuchsias were repeated in the same manner as above, except that the freezing temperature in this experiment was maintained at -5°C for four hours.
- the results listed in Table IV indicate that PPC707, Golden Frost Free, and PS/PEG are not individually as effective as is THFA for fuchsias.
- Fuchsias sprayed with 5% THFA for only 2 hours prior to exposure for freezing conditions also survived.
- 1,2-propanediol a poly-alcohol
- glass forming tendency and stability in the amorphous state which are the properties required for good cryoprotecting agents.
- the frost damage test results in Table X show that tetrahydrofurfuryl alcohol and amine are better antifrost agents than 1,2-propanediol.
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Detergent Compositions (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Thermotherapy And Cooling Therapy Devices (AREA)
Abstract
Aqueous plant cryoprotectant composition containing between about 0.005 and 25 wt.% tetrahydrofurfuryl alcohol, tetrahydrofurfuryl amine or mixtures thereof and method of increasing the resistance of plant tissue to damage caused by freezing conditions comprising applying to the plant tissue surfaces at ambient non-freezing temperatures an aqueous solution containing an effective amount of tetrahydrofurfuryl alcohol, tetrahydrofurfuryl amine or mixtures thereof.
Description
CRYOPROTECTANT COMPOSITION
BACKGROUND OF THE INVENTION
This invention relates to plant cryoprotectant compositions and methods for preventing damage to plants caused by exposure to freezing temperatures for limited periods.
Freezing temperatures are a major cause of damage to plants and plant tissue. According to one estimate, approximately $1.5 billion of agricultural products are annually lost to freezing temperatures in the United States. In Florida alone, more than $3 billion of frost- damage to citrus trees has been reported over the last five years.
Numerous methods have been attempted to prevent damage to plants and plant tissue caused by freezing temperatures. One such method involves the development of hardy plant species, a laborious, time consuming and costly proposition. Other methods involve the use of fogs, smoke, protective foams, bags and the like to prevent the escape of heat from the plant. Still other methods involve the use of wind machines, heaters and/or irrigation. However, none of these approaches has proven to be both effective and commercially practical.
A different approach that has been attempted to prevent frost damage to plants involves treatment of the plant with a cryoprotectant composition. During the last 75 years, many compounds have been identified which exert cryoprotection when added to plant tissues. However, in order to be practically useful, such a composition must be non-toxic to plants, environmentally acceptable and rela- tively inexpensive. The center of research interest in this
field has changed from early emphasis on small molecules that penetrate the cell, to more recent exploration of protective effects derived from very large non-penetrating compounds as they affect the properties of the external" solution. However, none of these compounds are currently in wide-spread use. A few successful trial cases have been reported, but the chemicals employed have not been generally accepted.
SUMMARY OF THE INVENTION Among the objects of the invention, therefore, may be noted the provision of a plant cryoprotectant composition that increases the hardiness of plants and plant tissue. Another object of this invention is the provision of a plant cryoprotectant composition which is relatively inexpensive, non-toxic, and environmentally acceptable. A further object of this invention is the provision of a method for the cryoprotection of plants and plant tissue.
Briefly, therefore, the present invention is directed to an aqueous plant and plant tissue cryoprotec¬ tant solution containing between about 0.005 and about 25 wt.% of a cryoprotectant component selected from the group consisting of tetrahydrofurfuryl alcohol, tetrahydro¬ furfuryl amine and mixtures thereof. The invention is further directed to a method of increasing the resistance of plant tissue to damage caused by freezing conditions comprising applying to the plant tissue surfaces at an ambient non-freezing temperature, an aqueous solution containing between about 0.005 and about 25 wt.% of a cryoprotectant component selected from the group consisting of tetrahydrofurfuryl alcohol, tetrahydro¬ furfuryl amine and mixtures thereof at least about 4 hours prior to the onset of such freezing conditions.
Other objects and features of this invention will be in part apparent and in part pointed out hereinafter.
DESCRIPTION OF THE INVENTION
In accordance with this invention, a plant cryoprotectant composition has been discovered which comprises an aqueous solution containing a cryoprotectant component selected from the group consisting of tetrahydro¬ furfuryl alcohol, tetrahydrofurfuryl amine or mixtures thereof. Preferably, the composition comprises between about 0.005 and about 25 wt% of the cryoprotectant component and most preferably comprises between about 0.1 and about 5 wt% of the cryoprotectant component.
Tetrahydrofurfuryl alcohol is a colorless, high boiling, primary alcohol having the following structure:
Tetrahydrofurfuryl amine is a colorless, high boiling primary amine having the following structure:
Although both tetrahydrofurfuryl alcohol and tetrahydrofurfuryl amine exhibit plant cryoprotectant properties, tetrahydrofurfuryl alcohol is preferred in accordance with the present invention. Tetrahydrofurfuryl alcohol is produced by hydrogenation of furfuryl alcohol and is marketed under the name THFA. As expected on the
basis of its structure, tetrahydrofur uryl alcohol exhibits behavior characteristics of both alcohols and ethers. Due to its cyclic ether structure, tetrahydrofurfuryl alcohol possesses distinctly unique solvent properties which are desirable for cryoprotecting agents, such as:
1.) low volatility (vapor pressure 2.3 mm Hg at
39°C) , 2.) non-damaging and non-toxic, 3.) biodegradable, 4.) easily absorbable,
5.) ability to penetrate membranes, 6.) considerable solubility in water in addition, to its ability to form multiple hydrogen bonds, 7.) ability to dissolve electrolytes.
The resistance of plants and plant tissue to frost and low temperatures, including subfreezing temperatures, are increased for limited periods through the application of the cryoprotectant composition of this invention by spraying or dipping. For spraying, any suitable plant spray apparatus suitable for spraying aqueous solutions may be employed. The plants to be treated are thoroughly sprayed so that all of the plant tissue surfaces are completely covered. Due to the size or shape of a plant, a single application may require two or more sprayings.
The cryoprotectant composition is applied at ambient, non-freezing temperatures, i.e., the temperature of the air surrounding the plant or plant tissue is above 32βF. Preferably, the cryoprotectant composition is applied when the ambient air temperature is at least 35°F.
(5)
The cryoprotectant composition may be formulated and supplied to the user in concentrated form and diluted to the desired strength prior to application to the plant. No special handling or mixing steps are required. THFA and tetrahydrofurfuryl amine are stable in aqueous solution.
Moreover, these compositions are stable to light and do not need to be stored in an opaque container nor prepared immediately prior to application.
Since an aqueous THFA or tetrahydrofurfuryl amine solution may not completely wet the surfaces of leaves of plants having waxy leaves, for some applications it is preferred that the cryoprotectant composition include a non-ionic surfactant. Two different surfactants, polyoxy¬ ethylene sorbitan monolaurate (Tween 20) and polyoxyethylene sorbitan monooleate (Tween 80) have been found to improve the wetability of the composition and thereby improve its effectiveness. When non-ionic surfactants are used, it is preferred that the cryoprotectant composition contain between about 0.05 and about 0.5 wt.% non-ionic surfactant. The e fectiveness of the cryoprotectant composi¬ tion is not diminished by the inclusion of such compounds as organic/inorganic fertilizers, pesticides, plant hormones, growth regulators, other polymers, and coating materials. For instance, it has been found that the composition is compatible with urea; the introduction of urea via a 1:1 THFA:UREA mixture, did not affect the wet¬ ability or the effectiveness of the agent. Nevertheless, it is presently preferred that the cryoprotectant composi¬ tion have an essential absence of pesticides. Although the cryoprotectant composition of this invention may be applied to the plants immediately prior to exposure to freezing conditions, it is preferred that the composition be applied between about 4 hours and 1 week
prior thereto. Moreover, for optimal results it is preferred that the cryoprotectant composition be applied twice prior to the onset of freezing temperatures, the first application being made between about several days and about one week prior to the onset of freezing temperatures and' constituting a conditioning application. The second appli¬ cation is then preferably made a sufficient period prior to the onset of freezing temperatures to permit absorption of the composition, e.g., at least about 4 hours. For maximum protection during the spring frost season, it may be desirable to apply the cryoprotectant composition weekly to minimize any damage that might be caused by a sudden occurrence of freezing temperatures. Nevertheless, due to this agent's high penetrability into some plant tissues, it may be possible to apply this inven¬ tion immediately before the exposure to freezing tempera¬ tures if the plant can tolerate a high concentration of the agent.
The following examples illustrate the invention. All percentages are by weight unless otherwise indicated.
EXAMPLE 1 A. Materials and Methods
Plants were sprayed with the indicated antifrost agents and set out at room temperature for a period of time for conditioning. Several hours prior to exposure to freezing temperatures, a second application of the antifrost agent was given to some of the plants. The period between the first application and the second application is referred to hereinafter as the conditioning time and the period between the second application and the placement in the freezer, as described below, is referred to as the absorp¬ tion time. For those instances in which there was only a
single application and the elapsed time between the applica¬ tion and placement in the freezer exceeded 12 hours, that period is referred to as conditioning time. If the period was less than 12 hours, it is referred to as absorption time.
The pots holding the conditioned plants were fitted into a polyurethane insulated box having circular openings in the top. Each opening was sized to snugly receive a single pot holding a conditioned plant and the box had sufficient depth so that substantially the entire pot was contained within the insulated box while the condi¬ tioned plant stood outside the box. The box was heated by circulation of room temperature air through its interior. The insulated box holding the plants were placed inside a commercial freezer that was modified to achieve dynamic temperature control to within 0.01°C. The air temperature inside the freezer was monitored on a CRT screen and also recorded on a chart recorder. The air temperature inside the freezer was gradually reduced (4°C/hr) from an initial temperature (10°C) until the set point temperature was achieved. The set temperature was maintained for a period of three or four hours and then gradually warmed (4°C/hr) to a final temperature (i.5°C) . During the period that the plant was inside the freezer, the insulated box protected the roots from freezing somewhat the same, but less effi¬ ciently, as the ground does in nature.
After removal from the freezer, the plants were inspected and a binary result recorded, i.e., either the plants survived or were permanently damaged. Visual obser- vance could easily differentiate the effectiveness of the various antifrost agents tested. For each test, if all of the plants survived the initial cold-, the plants were removed from the freezer, the pots were allowed to return
(8)
to room temperature and the plants were then returned to the freezer where the air temperature was then set at a lower temperature than the previous run. This process was repeated until a temperature was reached at which some of the plants did not survive. The useable temperature range and relative effectiveness of the antifrost agents was thus determined.
The maximum time for exposure to freezing temperatures was limited to 6 hours because beyond -that, the pots could not be kept from freezing. Instead, the tests in which plants survived exposure for 6 hours were repeated a few times to determine whether the plants could survive longer exposure, like those encountered under actual conditions in the field. The antifrost agents evaluated were the following:
1. tetrahydrofurfuryl alcohol dissolved in deionized (Dl) water to make 0.5% and 5% THFA aqueous solutions;
2. 0.1 parts of a surfactant, polyoxyethylene sorbitan monolaurate (Tween 20) and 0.5 parts tetrahydro¬ furfuryl alcohol dissolved in 99.4 parts Dl water to make an aqueous 0.5% THFA + 0.1% Tween 20 solution;
3. 0.5 parts urea and 0.5 parts tetrahydro¬ furfuryl alcohol dissolved in 99 parts Dl water to make an aqueous 0.5% THFA + 0.5% urea solution.
4. polyethyleneglycol polysiloxane copolymer having a PG/PS monomer ratio of 5.3/1 prepared according to the method set forth in D.J. Bannister, et al "Water Soluble Siloxane: Poly (Erthylene Glycol) Comb Polymer", Journal of Polymer Science: Polvmer Letters Ed.. Vol. 23, p.
465-467 (1985), dissolved in Dl water to make aqueous 0.5% and 5% PS/PEG solutions;
(9) .
5. A non-ionic surfactant GOLDEN FROST FREE (Plant Products Corporation; Vero Beach Florida), dissolved in Dl water to make aqueous 0.3% and 0.29% Golden Frost Free solutions; 6. a polyoxyethylenated polyoxypropylene copolymer (molecular weight 4500) obtained from Diamond Shamrock Corporation under the trademark Monolan 4500, dissolved in Dl water to make aqueous 0.3% and 0.4% solutions designated PPC707; 7. equal parts tetrahydrofurfuryl alcohol and polyethyleneglycol polysiloxane copolymer (subpart 4, above) dissolved in Dl water to make an aqueous 0.25% THFA + 0.25% PS/PEG solution ("0.5% mixture");
8. tetrahydrofurfuryl amine dissolved in Dl water to make an 0.5% tetrahydro urfuryl aqueous solution.
9. furfuryl alcohol dissolved in Dl water to make an 0.5% furfuryl alcohol aqueous solution.
10. 1,2-propanediol dissolved in Dl water to make an 0.5% 1,2-propranediol aqueous solution.
B. Evaluation of the Antifrost Agents with Impatiens Garden plants of the impatiens variety were sprayed with various antifrost agents listed in part A (as indicated in Table I) . Each plant was treated twice with the respective antifrost agent and was allowed five days between the two applications for conditioning. After the second spray, a six hour absorption period was provided. The plants were then placed in the freezer and the tempera¬ ture was lowered to the set point temperature (-3.5°C), maintained there for three hours, and then raised to 8°C over a three hour period.
As shown in table I, the impatiens treated with 0.5% THFA and 0.5% PS/PEG survived whereas the plants treated with PPC707 and those exposed without any antifrost treatment died.
(10)
TABLE I
FREEZING DAMAGE TEST RESULTS FOR IMPATIENS EXPOSED TO -3.5°C FOR THREE HOURS
Antifrost Agent Conditioning/Absorption Time Results
Blank - 7 - Died
0.5% PS/PEG 5 days/6 hours Survived
0.5% THFA 5 days/6 hours Survived
0.3% Golden Frost Free 5 days/6 hours Died
0.3% PC707 5 days/6 hours Died
C. Second Evaluation of the Antifrost Agents with Impatiens The procedure provided in part B was repeated, except that some of the plants were given a single application of the compositions having a higher concentra¬ tion of the antifrost agents to determine whether these agents are effective with only one treatment. The remainder of the plants were given two applications as indicated in Table II. The results listed in Table II indicate that impatiens treated once with either 5% PS/PEG or 5% THFA survived the freezing temperatures. Again, plants treated with 0.5% PS/PEG or 0.5% THFA survived, while those treated with PPC707 or Golden Frost Free, like the ones left without any antifrost protection, did not survive.
TABLE I I
FROST DAMAGE TEST FOR IMPATIENS EXPOSED TO -3.5°C FOR THREE HOURS
Antifrost Agent Conditioning/Absorption Time Results Blank - / - Died
0.5% PS/PEG - /6 hours Died
5% PS/PEG - /6 hours Survived
5% PS/PEG 2 days/ - Survived
0.5% Mixture 5 days/6 hours Survived 0.5% THFA 7 days/6 hours Survived
5% THFA - /6 hours Survived
0.4% PPC707 7 days/6 hours Died
0.29% Golden Frost Free 7 days/6 hours Died
D. Evaluation of the Antifrost Agents with Fuchsias The procedure in part B was repeated except that the garden plants employed for this test were fuchsias.
The fuchsias were treated with various antifrost agents (as indicated in Table III) and then exposed to a freezing temperature of -4°C for three hours. The test results in Table III show that Golden Frost Free, 0.5% THFA, 0.5%
PS/PEG, and 5% THFA are effective in protecting fuchsias from the ravages of frost.
TABLE III
FROST DAMAGE TESTS FOR FUCHSIAS EXPOSED TO -4°C FOR THREE HOURS
Antifrost Agent Conditioning/Absorption Time Results
Blank - / - Died
Golden Frost Free 5 days/4 hours Survived
0.5% THFA 5 days/4 hours Survived 0.5% PS/PEG 5 days/4 hours Survived
5% THFA - /4 hours Survived
5% PS/PEG - /4 hours Died
(12)
E. Second Evaluation of the Antifrost Agents with Fuchsias
Freezing survival tests for fuchsias were repeated in the same manner as above, except that the freezing temperature in this experiment was maintained at -5°C for four hours. The results listed in Table IV indicate that PPC707, Golden Frost Free, and PS/PEG are not individually as effective as is THFA for fuchsias. Fuchsias treated with 5% THFA for 4 hours prior to their exposure to -5°C survived, as did those treated twice with 0.5% THFA. Fuchsias sprayed with 5% THFA for only 2 hours prior to exposure for freezing conditions also survived.
TABLE IV
FREEZING SURVIVAL TESTS OF FUCHSIAS TESTED AT -5°C FOR FOUR HOURS
Antifrost Agent Conditioning/Absorption Time Results
7 days/4 hours Died 7 days/4 hours Died 7 days/4 hours Died 7 days/4 hours Died
7 days/4 hours Died
- /4 hours Survived
F. Third Evaluation of the Antifrost Agents with Fuchsias
Here, the freezing temperature was lowered to -6°C and maintained there for 4 hours. The fuchsias tested in this example were those which survived the previous freezing tests. The purpose of this test was to determine whether the plants could survive longer exposures, like those encountered under actual conditions in the field.
TABLE V
FREEZING SURVIVAL TESTS FOR FUCHSIAS EXPOSED TO -6°C
Antifrost Agent Total Exposure Time Results 0.5% THFA 8 hours Survived
0.5% THFA 12 hours Survived
5% THFA 8 hours Survived
5% THFA 12 hours Survived
G. Evaluation of the Antifrost Agents with Coleuses The garden plant coleus is very delicate and sensitive to freezing temperatures due to the high water content of its tissues. A set of six coleuses treated with different antifrost agents was exposed to -7°C for four hours. The results of this test are listed in Table VI. The plants treated twice with 0.5% THFA survived. Coleuses treated with 5% THFA showed signs of antifrost agent rejec¬ tion and were not included in this frost survival test.
TABLE VI
FREEZING SURVIVAL TEST FOR COLEUSES EXPOSED TO -7°C FOR FOUR HOURS
Antifrost Agent Conditioning/Absorption Time Results
Blank - / - Died 0.29% Golden Frost
Free 3 days/2 hours Died 0.3% PPC707 3 days/2 hours Died
0.5% Mixture 3 days/2 hours Died
0.5% THFA 3 days/2 hours Survived
0.5% THFA 3 days/2 hours Survived
H. Evaluation of Cryoprotectant Composition containing a Surfactant or Urea.
The poor wetability of aqueous THFA solutions on some of the waxy-leaved plants such as peppers or tomatoes delays absorption of this solution into the plant tissues. In order to improve the wetability, two different surfac¬ tants selected from the EPA inert compound list were tested. Both surfactants dramatically improved the cryoprotectant composition's wetabiltiy. Both surfactants contained potential known cryoprotectant ingredients such as sorbitol and polyoxyethylene. Since Tween 20 (polyoxyethylene sorbi¬ tan monolaurate) was found to mix with the cryoprotectant composition much more easily than Tween 80 (polyoxyethylene - sorbitan monooleate) , tween 20 was used in the frost damage tests. Six tomatoes treated with aqueous 0.5% THFA solu¬ tions containing different additives were exposed to -5°C for 4 hours. The results of this test are listed in Table VII. The incorporation of a mere 0.1% surfactant not only dramatically improved the wetability but also reduced the absorption time of the agent. The addition of urea did not seem to improve the effectiveness of the agent, but the test results do indicate that this agent is compatible with the fertilizer.
TABLE VII
FREEZING SURVIVAL TEST RESULTS FOR TOMATOES EXPOSED TO -5°C FOR FOUR HOURS
Antifrost Aoent Additive Results
0.5% THFA None Survived 0.5% THFA 0.1% Tween 20 Survived
0.5% THFA 0.5% urea Survived
0.5% THFA 0.5% urea + 0.1% Tween 20 Survived
Blank Blank Died
I. Evaluation of the Antifrost Agent with Peppers
In this experiment, peppers were treated with different antifrost agents and were exposed to -6°C for 4 hours. The test results listed in Table VIII show that the plants treated with Golden .Trost Free, 0.5% THFA with 0.1% Tween 20, and 0.5% THFA mixed in with 0.1% Tween 20 and 0.5% urea, survived. One Interesting observation here is that 0.5% THFA did not prevent freezing damage to these plants. The reason may be the poor wetability of THFA alone on this plant. However, the effectiveness of THFA only on pepper plants was confirmed through additional tests.
TABLE VIII
FREEZING SURVIVAL TEST RESULTS FOR PEPPER TREATED WITH VARIOUS ANTIFROST AGENTS AFTER EXPOSURE TO -6°C FOR 4 HOURS
Antifrost Agent Conditioning/Absorption Time Results
0.5% THFA 3 days/3 hours Died
0.5% THFA + 0.1%
Tween 20 3 days/3 hours Survived 0.5% THFA + 0.5% urea
+ 0.1% Tween 20 3 days/3 hours Survived
0.29% Golden Frost
Free 3 days/3 hours Survived
J. Further Evaluation of the Cryoprotectant Compositions with Impatiens
Freezing survival tests for impatiens were continued using aqueous .solutions of other furfuryl group compounds to evaluate their effectiveness as antifrost agents. The impatiens used in this evaluation and in the evaluation of subpart K, infra were grown in a much warmer environment than the evaluations reports in subparts A-I,
supra. None of the impatiens treated with antifrost agents and conditioned at room temperature for 3 to 10 days survived freezing temperatures of -3.5°C to -6.0°C, indica¬ ting that these plants should have been acclimatized at near freezing temperatures before they were exposed to f eezing conditions.
Two impatiens were sprayed with 0.5% THFA, two with 0.5% tetrahydrofurfuryl amine, and one with 0.5% furfuryl alcohol. A control blank was not treated. After standing for 7 days for conditioning, these plants were acclimated to 1°C for 24 hours. Then the plants were sprayed once more with the respective antifrost composi¬ tions and sufficient time was allowed for the agents to be absorbed completely. Finally the plants were exposed to a temperature of -3.5°C for 4 hours. As shown in Table IX, the plants treated with THFA or tetrahydrofurfuryl amine survived whereas the others died.
TABLE IX
FREEZING SURVIVAL TESTS FOR IMPATIENS TREATED WITH AQUEOUS SOLUTION OF FURFURYL GROUP COMPOUND AFTER EXPOSURE TO -3.5°C FOR 4 HOURS
Antifrost Agent Conditioning/Acclimating/Absorption Time Result
Died Survived Survived Survived
K. Further Evaluation of the Cryoprotectant Compositions with Impatiens
The procedure outlined in subpart J was repeated except that another potential candidate, 1,2-propanediol was compared with THFA and tetrahydrofurfuryl amine.
1,2-propanediol, a poly-alcohol, is the best reported to date for. its glass forming tendency and stability in the amorphous state, which are the properties required for good cryoprotecting agents. The frost damage test results in Table X show that tetrahydrofurfuryl alcohol and amine are better antifrost agents than 1,2-propanediol.
TABLE X
FREEZING SURVIVAL TEST RESULTS FOR IMPATIENS AFTER EXPOSURE TO -4°C FOR 4 HOURS
Antifrost Agent Conditioning/Acclimating/Absorption Time Result
Blank Died
0.5% 1,2-propanediol Died 0.5% 1,2-propanediol Died 0.5% Tetrahydro¬ Survived furfuryl Amine 0.5% THFA Survived 0.5% THFA
Survived
In view of the above, it will be seen that the several objects of the invention are achieved.
As various changes could be made in the above compositions and methods without departing from the scope of the invention, it is intended that all matter contained in the above description shall be interpreted as illustra¬ tive and not in a limiting sense.
Claims
1. An aqueous plant and plant tissue cryoprotectant solution containing a non-ionic surfactant and between about 0.005 and about 25 wt.% of a cryoprotectant component selected from the group consisting of tetrahydrofurfuryl alcohol, tetrahydrofurfuryl amine and mixtures thereof.
2. An aqueous plant and plant tissue cryoprotectant solution containing a non-ionic surfacant and between about 0.005 and about 25 wt.% of tetrahydrofurfuryl alcohol.
3. An aqueous plant and plant tissue cryoprotectant solution containing a non-ionic surfacant and between about 0.1 and about 5 wt% tetrahydrofurfuryl alcohol.
4. An aqueous solution as set forth in claim 1, 2 or 3 wherein said aqueous solution contains between about 0.05 and about 0.5 wt.% of a non-ionic surfactant.
5. An aqueous solution as set forth in claim 4 wherein said non-ionic surfactant is polyoxyethylene sorbitan monolaurate or polyethylene sorbitan monooleate.
6. A method of increasing the resistance of plant tissue to damage caused by freezing conditions comprising applying to the plant tissue surfaces at ambient non-freezing air temperatures at least about four hours prior to exposure to such freezing conditions an aqueous solution containing an effective amount of tetrahydrofurfuryl alcohol, tetrahydrofurfuryl amine or mixtures thereof as a cryoprotectant component.
7. A method for preventing damage to plants caused by exposure to freezing conditions comprising applying to the plant surfaces at ambient non-freezing temperatures at least about four hours prior to exposure to such freezing conditions an aqueous cryoprotectant solution containing an effective amount of tetrahydrofurfuryl alcohol.
8. A method as set forth in claim 6 or claim 7 wherein the aqueous solution contains between about 0.005 and about 25 wt% tetrahydrofurfuryl alcohol.
9. A method as set forth in claim 6 or claim 7 wherein the aqueous solution contains between about 0.1 and about 5 wt% tetrahydrofurfuryl alcohol.
10. A method as set forth in claim 6 or claim 7 wherein said aqueous solution contains a non-ionic surfactant.
11. A method as set forth in claim 6 or claim 7 wherein said aqueous solution contains between about 0.05 and about 0.5 wt.% non-ionic surfactant.
12. A method as set forth in claim 11 wherein said non-ionic surfactant is polyoxyethylene sorbitan monolaurate or polyethylene sorbitan monooleate.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019900700489A KR920009404B1 (en) | 1988-07-07 | 1989-06-28 | Method of protecting crops form being damaged by cold weather |
BR898907528A BR8907528A (en) | 1988-07-07 | 1989-06-28 | CRIO-PROTECTIVE COMPOSITION |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US216,126 | 1988-07-07 | ||
US07/216,126 US4886543A (en) | 1988-07-07 | 1988-07-07 | Cryoprotectant composition |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1990000350A1 true WO1990000350A1 (en) | 1990-01-25 |
Family
ID=22805804
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1989/002818 WO1990000350A1 (en) | 1988-07-07 | 1989-06-28 | Cryoprotectant composition |
Country Status (7)
Country | Link |
---|---|
US (1) | US4886543A (en) |
EP (1) | EP0428540A4 (en) |
JP (1) | JPH04500201A (en) |
KR (1) | KR920009404B1 (en) |
AU (1) | AU629226B2 (en) |
BR (1) | BR8907528A (en) |
WO (1) | WO1990000350A1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5176279A (en) * | 1989-08-15 | 1993-01-05 | Australian Stamping Foils Pty. Ltd. | Container cap |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5244864A (en) * | 1988-07-07 | 1993-09-14 | Great Lakes Chemical Corp. | Methods for protection and treatment of plants exposed to chilling temperatures |
AU8930191A (en) * | 1990-11-14 | 1992-06-11 | Great Lakes Chemical Corporation | Methods for protection and treatment of plants exposed to chilling temperatures |
US5224982A (en) * | 1992-03-06 | 1993-07-06 | Great Lakes Chemical Corporation | Methods and compositions for treating plants exposed to water deprivation stress |
US5224981A (en) * | 1992-03-17 | 1993-07-06 | Great Lakes Chemical Corporation | Methods and compositions for treating plants exposed to salt |
US5268017A (en) * | 1992-03-02 | 1993-12-07 | Great Lakes Chemical Corporation | Methods and compositions for treating plants exposed to ozone |
WO1993017556A1 (en) * | 1992-03-02 | 1993-09-16 | Great Lakes Chemical Corporation | Methods and compositions for treating plants exposed to stresses |
SA06270491B1 (en) * | 2005-12-27 | 2010-10-20 | سينجنتا بارتيسبيشنز ايه جي | Herbicidal CompositionComprising of 2,2-Dimethyl-Propionic Acid 8-(2,6-Diethyl-4-Methyl-Phenyl)-9-Oxo-1,2,4,5-Tetrahydro-9H-Pyrazolo[1,2 d] [1,4,5]Oxadiazepin-7-yl Ester and an alcohol |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4219965A (en) * | 1979-03-02 | 1980-09-02 | Catalytic Generators, Inc. | Frost damage prevention in plant tissue |
US4618442A (en) * | 1985-10-07 | 1986-10-21 | Geary Robert J | Plant cryoprotection |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2119653A (en) * | 1982-05-17 | 1983-11-23 | Ici Plc | Pesticidal compositions |
DE3413996A1 (en) * | 1984-04-13 | 1985-10-24 | Bayer Ag, 5090 Leverkusen | TETRAHYDROFURAN-2-YLMETHYLAMINE |
DE3730499A1 (en) * | 1987-02-10 | 1988-08-18 | Bayer Ag | AMINOMETHYLTETRAHYDROFURANE |
-
1988
- 1988-07-07 US US07/216,126 patent/US4886543A/en not_active Expired - Fee Related
-
1989
- 1989-06-28 JP JP1507550A patent/JPH04500201A/en active Pending
- 1989-06-28 EP EP19890908044 patent/EP0428540A4/en not_active Withdrawn
- 1989-06-28 KR KR1019900700489A patent/KR920009404B1/en not_active IP Right Cessation
- 1989-06-28 BR BR898907528A patent/BR8907528A/en not_active Application Discontinuation
- 1989-06-28 WO PCT/US1989/002818 patent/WO1990000350A1/en not_active Application Discontinuation
- 1989-06-28 AU AU38670/89A patent/AU629226B2/en not_active Ceased
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4219965A (en) * | 1979-03-02 | 1980-09-02 | Catalytic Generators, Inc. | Frost damage prevention in plant tissue |
US4618442A (en) * | 1985-10-07 | 1986-10-21 | Geary Robert J | Plant cryoprotection |
Non-Patent Citations (6)
Title |
---|
CHEMICAL ABSTRACTS, Volume 81, No. 9, 2 September 1974 (Columbus, Ohio, USA), ANON. "Tetrahydrofurfuryl alcohol. Exception from Requirement of a Tolerance", see page 313, column 2, the Abstract No. 48636m, Fed. Regist., 1974, 39(58), 11080-1 (Eng). * |
Chemtech, Volume 14, 1984, B. ALLEGRETTO, "Using Polymers for Agricultural Frost Protection", pages 152-155. * |
QO Chemicals, Inc. Bulletin 206-B-I, "QO Tetrahydrofurfuryl Alcohol/THFA. General Information-Properties-Applications Handling", pages 1-17. * |
QO Chemicals, Inc. Bulletin 206-B-II. 206-B-II, "QO Tetrahydrofurfuryl Alcohol/THFA. An Environmentally Acceptable, Biodegradable, Water Miscible Solvent", pages 1-8. * |
See also references of EP0428540A4 * |
The Merck Index, Tenth Edition, 1983, Merck & Co., Inc. Rahway, N.J. USA, page 1318, second column, 9038. * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5176279A (en) * | 1989-08-15 | 1993-01-05 | Australian Stamping Foils Pty. Ltd. | Container cap |
Also Published As
Publication number | Publication date |
---|---|
EP0428540A1 (en) | 1991-05-29 |
AU3867089A (en) | 1990-02-05 |
BR8907528A (en) | 1991-06-11 |
EP0428540A4 (en) | 1993-03-10 |
KR900701163A (en) | 1990-12-01 |
US4886543A (en) | 1989-12-12 |
AU629226B2 (en) | 1992-10-01 |
JPH04500201A (en) | 1992-01-16 |
KR920009404B1 (en) | 1992-10-16 |
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