WO1981003224A1 - Flow-through optical analyzer - Google Patents
Flow-through optical analyzer Download PDFInfo
- Publication number
- WO1981003224A1 WO1981003224A1 PCT/US1981/000479 US8100479W WO8103224A1 WO 1981003224 A1 WO1981003224 A1 WO 1981003224A1 US 8100479 W US8100479 W US 8100479W WO 8103224 A1 WO8103224 A1 WO 8103224A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- particles
- fluid
- path
- inlet
- area
- Prior art date
Links
- 230000003287 optical effect Effects 0.000 title description 5
- 239000002245 particle Substances 0.000 claims abstract description 53
- 239000012530 fluid Substances 0.000 claims abstract description 19
- 238000000034 method Methods 0.000 claims abstract description 9
- 238000003384 imaging method Methods 0.000 claims description 11
- 238000004458 analytical method Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims 4
- 239000000523 sample Substances 0.000 claims 3
- 230000007423 decrease Effects 0.000 claims 2
- 239000012472 biological sample Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 abstract description 35
- 210000000601 blood cell Anatomy 0.000 abstract description 7
- 210000004369 blood Anatomy 0.000 description 11
- 239000008280 blood Substances 0.000 description 11
- 239000002131 composite material Substances 0.000 description 4
- 230000009977 dual effect Effects 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- KKEBXNMGHUCPEZ-UHFFFAOYSA-N 4-phenyl-1-(2-sulfanylethyl)imidazolidin-2-one Chemical compound N1C(=O)N(CCS)CC1C1=CC=CC=C1 KKEBXNMGHUCPEZ-UHFFFAOYSA-N 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000004886 process control Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000003909 pattern recognition Methods 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000011218 segmentation Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
- G01N15/147—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N2015/1486—Counting the particles
Definitions
- This invention relates to a chamber for analysis 5 of flowing particles and a system incorporating such chamber.
- the still frame images of the flow stream are provided by imaging the magnified image of the stream on a CCD (charge coupled device) camera from which still frame images are taken and analyzed in digital form.
- CCD charge coupled device
- the still frame images may be enhanced with the digital image enhancement tech ⁇ niques which have been developed for satellite pictures and the individual frames may .
- Preferably information derived from still frame images is combined to provide composite information reflecting the content of the multiple still frame images- and/or predetermined reference images, and the composite information thus obtained may be used in a variety of ways.
- info ' rma- tion may be printed out, for instance, to advise a hematologist about composite measurements made from a blood sample.
- the composite measurements may be used by process control, such as pressure in a ho ogenizer, temperature in a crystal- lizer, or nutrient feed rate in a microbial culture where the system monitors particle size or number.
- the invention may be used for analysis of a varity of optically perceptible particles moving in a stream, both biological parti ⁇ cles, such as cells in blood or cells, bacteria, casts and crystals in urine or particles in gas analyzers, etc. , and the output of these measurements may be employed for process control, such as dispensing nutrients into a stream containing microorganisms as mentioned above, the control of the growth of polymers and crystals, etc.
- the information which is provided may be corre ⁇ lated readily to the original volume of blood sample from which the still frame images are made by a variety of methods to calibrate the results for both particle size and concentration. For instance calibration may be accomplished by adding calibrator particles to the original blood sample in a known concentration so that the calibrator particles may be counted independently of the normal blood cells to provide volume calibration for the normal blood cells. Alternatively, calibration may be accomplished by providing a cross-hatch of fixed dimension in the field of view.
- the flow stream of particles to be magnified and imaged is preferably provided in a flow chamber which moves the particles in a stream which is approximately the thickness of the thickest particles and many times wider than the widest particles, for instance, more than one hundred times as wide as the widest particles.
- the flow chamber might be designed to create turbulent flow to permit asymmetric particles to be viewed from multiple directions.
- the flow chamber may be designed to orient the particles.
- the flow stream in the imaging area has a cross-sectional area of minimum shear which is not substantially larger than the minimum cross-sectional
- mini- mum velocity gradient so that a particle moving in the stream tends to align itself with the direction of the stream much as a log floating down a river will align itself with the direction of flow where there is a flow gradient.
- FIG. 1 is a perspective view of apparatus for examining a flow stream in accordance with this invention.
- Fig. 2 is a plan view of the flow chamber in Fig. 1.
- Fig. 3 is a cross-sectional *view of the apparatus of Fig. 2 taken on the plane indicated at 3-3.
- Fig. 4 is a schematic diagram of the electronic processor employed by the apparatus of Fig. 1.
- the apparatus shown therein includes a body 10 containing a flow chamber having an inlet 12 for a blood sample and an outlet 14. with a passageway 16 extending between them past an imaging area 18.
- the passageway 16 has an inlet with a conduit 20 adapted to be connected to a volume of saline solution 22.
- the inlet 12 for the blood sample has a needle 24 in the passageway 16 downstream from the conduit 20 with the needle 24 connected to a container 26 adapted to hold the blood sample to be analyzed.
- the cross-sectional area of the passageway 16 becomes progressively smaller as the passageway extends from the blood inlet 12 to the imaging area 18 while at the same time the passageway 16 becomes much shallower. From the entrance to the imaging area 18 to the outlet 14 there is an increase in width in the passageway 16.
- the passageway 16 has a width and depth of about 5,000 microns at the blood inlet 12 and a width and depth of about 500 microns at midpoint 28, and a depth of 100 microns with a width exceeding 5,000 microns at the examination area 18.
- the flow stream through the examination area 18 is many times deeper than the largest cells which have a maximum dimension of about 20 microns, but with the flow passageway shaped in this way the blood stream entering through the opening 12 is confined to a stable flow path of minimum shear in the examination area 18, and the disc-like cells are oriented in that area with their maximum cross-sectional area visible in the plane of Fig. 2.
- the flow characteristics in the passageway 16 may be controlled by adjusting the fluid pressure in containers 22 and 26 either automatically or by adjust ⁇ ing the static heights thereof.
- a microscope 30 is focused on the examination area 18 and the examination area 18 is illuminated from below by a strobe light 32 which is preferably a U.S. Scientific Instrument Corporation Model 3018 containing a 2UP1.5 lamp.
- the output of the microscope 30 is focused on a CCD camera 34 which is preferably a CCD camera model number TC1160BD manufactured by RCA.
- the output of the CCD camera is converted to a series of still frame images, and suitable electronic processors -7-
- One pro ⁇ cessor which may be employed is the processor marketed as Image Analysis System Model C-1285 by Hamamatsu Systems, Inc., Waltham, Massachusetts.
- the output of the CCD camera is connected to an elec ⁇ tronic processor 36 which is illustrated in greater detail in Fig. 4 and includes a black and white tele ⁇ vision monitor 38 and a frame grabber 40 which stores still frame images of the subject viewed by the CCD camera.
- the frame grabber is preferably a Model FG08 frame grabber made by the Matrox Corporation of Montreal, the output of which is supplied to a video refresh memory 42 Model RGB 256 made by Matrox Corpo ⁇ ration which are both coupled to the multibus 44 of the central processing unit 46 which is preferably an Intel 80/20 computer.
- the multibus is also coupled to a 48K random access memory 48 of Electronic Solutions, Inc., and a 16K dual port random access memory 50 Model RM 117 of Data Cube Corporation.
- the output of the video refresh memory is also coupled to a color monitor 52 which may be used to provide digitally enhanced video images of individual still frames for human examination.
- the second output of the dual port ram 50 is connected to a multibus 54 which is connected••to an Applied Micro Devices central processing unit 56, a 48K random access memory of Electronic Solutions, Inc. 58 and removable storage in the form of a floppy disc controller 60, such as an Advanced Micro Devices Model 8/8 and two units of Shugart floppy disc storage 62.
- a floppy disc controller 60 such as an Advanced Micro Devices Model 8/8 and two units of Shugart floppy disc storage 62.
- a wide variety of programming may be employed for processing pictures with the apparatus of Fig. 4 depending upon the particular task which user wishes to perform.
- the programming of the Hamamatsu System 1285 may be employed. Preferably, however, the programming is performed as follows:
- the tasks are first divided into those which must address each pixel in a given image and those which only address a small subset of the total. Since much time will be spent in the first class of tasks, they are programmed in assembly language on the interface processor 46 (the Intel 80/20 in Fig. 4). The output of these operations are then transferred to the host machine 56 via the dual ported ram 50. On the host side almost all of the necessary programming is more suitably done in a high level language such as Pascal (BASIC or FORTRAN could in principal be used also).
- the types of tasks that are done in the assembly language includes greyscale transformations, convolu ⁇ tions, and greyscale histogram claculations.
- the types of tasks done on the host side include overall control of the other devices, identification and segmentation of object of interest in the field of view, calculation of parameters associated with objects thus found, and formatting the output of results. Another way of considering this separation of tasks in this fashion is that tasks which must be performed at speeds great compared to a human operator are done in assembly. Tasks which are either complicated or which can operate at less than the maximum speed can be programmed in the higher language. Objects are found in a field of view primarily by setting a greyscale window function for values known to be characteristic of the desired object. These values can be established by prior knowledge or by well-known histogram techniques.
- an edge tracing program is invoked to outline the whole object associated with that pixel. Once the edge has been found, then many relevant parameters such as location, area, integrated optical density, and various moments can easily be calculated. Probability of membership in previously defined sub ⁇ groups can be determined from these derived parameters by means of standard decision theory. Definitions of blood cell morphology classifications are established by trained observers. These definitions are then used as the basis of the selected algorithms. Accuracy of the method is determined by comparison of machine results with those of trained observers examining the same samples. Output of the results can be programmed to be any of a variety of formats. Histograms, line plots, and tabular summaries are available for particu ⁇ lar needs.
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
Claims
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE813146423T DE3146423T1 (en) | 1980-05-02 | 1981-04-09 | FLOW-THROUGH OPTICAL ANALYZER |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US146064 | 1980-05-02 | ||
US06/146,064 US4338024A (en) | 1980-05-02 | 1980-05-02 | Flow analyzer and system for analysis of fluids with particles |
Publications (1)
Publication Number | Publication Date |
---|---|
WO1981003224A1 true WO1981003224A1 (en) | 1981-11-12 |
Family
ID=22515717
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1981/000479 WO1981003224A1 (en) | 1980-05-02 | 1981-04-09 | Flow-through optical analyzer |
Country Status (8)
Country | Link |
---|---|
US (1) | US4338024A (en) |
EP (1) | EP0050666B1 (en) |
JP (1) | JPH0352573B2 (en) |
AU (1) | AU546258B2 (en) |
CA (1) | CA1157157A (en) |
DE (1) | DE3146423T1 (en) |
GB (1) | GB2090427B (en) |
WO (1) | WO1981003224A1 (en) |
Cited By (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2510258A1 (en) * | 1981-07-22 | 1983-01-28 | Int Remote Imaging Systems Inc | PROCESS FOR ANALYZING THE DISTRIBUTION OF A REAGENT IN THE FORM OF PARTICLES AND A LIQUID IN A SUSPENSION |
FR2515352A1 (en) * | 1981-10-22 | 1983-04-29 | Int Remote Imaging Systems Inc | Detecting reagent in immunoassay procedure - by analysing reaction prod. suspension using electrical image processing |
DE3141984A1 (en) * | 1981-10-22 | 1983-05-05 | International Remote Imaging Systems, 91311 Chatsworth, Calif. | Method of analysing for particles |
EP0256867A2 (en) * | 1986-08-15 | 1988-02-24 | Hamilton-Thorn Research | Motility scanner and method |
FR2664983A1 (en) * | 1990-07-17 | 1992-01-24 | Centre Tech Ind Papier | DEVICE FOR THE CONTINUOUS DETECTION OF CONTRAST IMPURITIES CONTAINED IN A MOVING FLUID MATERIAL. |
EP0486747A2 (en) * | 1990-11-22 | 1992-05-27 | Toa Medical Electronics Co., Ltd. | Flow cell mechanism in flow imaging cytometer |
FR2681693A1 (en) * | 1991-09-24 | 1993-03-26 | Barrat Bertrand | DEVICE FOR DIMENSIONAL ANALYSIS OF PARTICLES POSITIONED IN A PLANE. |
EP0538551A2 (en) * | 1991-10-24 | 1993-04-28 | Toa Medical Electronics Co., Ltd. | Flow imaging cytometer |
EP0678742A2 (en) * | 1994-04-21 | 1995-10-25 | Hitachi, Ltd. | Monitoring method of stain solution for particle analysis and calibration method of particle analysis |
EP0683386A1 (en) * | 1994-05-17 | 1995-11-22 | TOA MEDICAL ELECTRONICS CO., Ltd. | Non-invasive blood analyzer |
US5598842A (en) * | 1993-09-03 | 1997-02-04 | Toa Medical Electronics Co., Ltd. | Non-invasive blood analyzer and method using the same |
DE19919608A1 (en) * | 1999-05-27 | 2000-11-30 | Roche Diagnostics Gmbh | Sample holder for the IR spectroscopy of sample liquids |
WO2004099773A1 (en) * | 2003-04-30 | 2004-11-18 | Pfizer Products Inc. | Automated in vitro cellular imaging assays for micronuclei and other target objects |
EP2397836A1 (en) * | 2010-05-06 | 2011-12-21 | Sony Corporation | Microparticle sorting apparatus, microchip and microchip module |
EP2331992A4 (en) * | 2008-10-02 | 2014-12-17 | Pixcell Medical Technologies Ltd | Optical imaging based on viscoelastic focusing |
Families Citing this family (118)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4408877A (en) * | 1979-04-10 | 1983-10-11 | Ernst Leitz Wetzlar Gmbh | Device for hydrodynamic focussing of a particle-suspension in a liquid flow cytophotometer |
US4612614A (en) * | 1980-09-12 | 1986-09-16 | International Remote Imaging Systems, Inc. | Method of analyzing particles in a fluid sample |
US4538299A (en) * | 1981-12-04 | 1985-08-27 | International Remote Imaging Systems, Inc. | Method and apparatus for locating the boundary of an object |
DE3315195A1 (en) * | 1982-04-29 | 1983-11-03 | International Remote Imaging Systems Inc., 91311 Chatsworth, Calif. | METHOD FOR ALIGNING PARTICLES IN A FLUID SAMPLE |
DE3315194A1 (en) * | 1982-04-29 | 1983-11-03 | International Remote Imaging Systems Inc., 91311 Chatsworth, Calif. | METHOD FOR SEPARATING PARTICLES FLOWING IN A FLUID SAMPLE |
DE3238353A1 (en) * | 1982-10-15 | 1984-04-19 | Max Planck Gesellschaft zur Förderung der Wissenschaften e.V., 3400 Göttingen | METHOD FOR SIMULTANEOUSLY QUANTITATIVE DETERMINATION OF BLOOD CELLS AND REAGENT THEREFOR |
JPH07114685B2 (en) * | 1983-07-28 | 1995-12-13 | 株式会社日立製作所 | Sewage treatment equipment |
US4596036A (en) * | 1983-08-31 | 1986-06-17 | The United States Of America As Represented By The United States Department Of Energy | Method and apparatus for fringe-scanning chromosome analysis |
US4661913A (en) * | 1984-09-11 | 1987-04-28 | Becton, Dickinson And Company | Apparatus and method for the detection and classification of articles using flow cytometry techniques |
US5229849A (en) * | 1984-09-17 | 1993-07-20 | University Of Delaware | Laser doppler spectrometer for the statistical study of the behavior of microscopic organisms |
US4833382A (en) * | 1986-06-06 | 1989-05-23 | Gibbs David L | Method and apparatus for use in microscope investigations |
CA1282164C (en) * | 1986-07-10 | 1991-03-26 | Walter Greenfield | System for microscopically analyzing fluids |
US4786165A (en) * | 1986-07-10 | 1988-11-22 | Toa Medical Electronics Co., Ltd. | Flow cytometry and apparatus therefor |
US4794453A (en) * | 1986-09-09 | 1988-12-27 | Web Printing Controls Co. | Method and apparatus for stroboscopic video inspection of an asynchronous event |
US4775515A (en) * | 1986-11-18 | 1988-10-04 | Cottingham Hugh V | Agglutinographic slide |
US4955720A (en) * | 1989-01-05 | 1990-09-11 | International Paper Company | On-line fiber orientation distribution measurement |
EP0486542B1 (en) * | 1989-08-10 | 1999-03-31 | International Remote Imaging Systems, Inc. | A method of differentiating particles based upon a dynamically changing threshold |
US5123055A (en) * | 1989-08-10 | 1992-06-16 | International Remote Imaging Systems, Inc. | Method and an apparatus for differentiating a sample of biological cells |
US5268966A (en) * | 1989-08-10 | 1993-12-07 | International Remote Imaging Systems, Inc. | Method of differentiating particles based upon a dynamically changing threshold |
JP2808321B2 (en) * | 1989-09-19 | 1998-10-08 | 東亜医用電子株式会社 | Cell analysis method and device |
US5101978A (en) * | 1989-11-27 | 1992-04-07 | The United States Of America As Represented By The Secretary Of The Army | Fluidic sorting device for two or more materials suspended in a fluid |
EP0433498B1 (en) * | 1989-12-13 | 1994-10-05 | Satake Engineering Co., Ltd. | Milling apparatus and system therefor |
US5000554A (en) * | 1990-05-23 | 1991-03-19 | Gibbs David L | Method and apparatus for use in microscope investigations with a carrier having exactly one x-y coordinate system reference mark |
US5159642A (en) * | 1990-07-13 | 1992-10-27 | Toa Medical Electronics Co., Ltd. | Particle image analyzing apparatus |
JP2939647B2 (en) * | 1990-07-24 | 1999-08-25 | シスメックス株式会社 | Automatic focus adjustment method for flow imaging cytometer |
US5170286A (en) * | 1991-02-19 | 1992-12-08 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Rapid exchange imaging chamber for stop-flow microscopy |
JP3121849B2 (en) * | 1991-02-27 | 2001-01-09 | シスメックス株式会社 | Flow image cytometer |
JP3084295B2 (en) * | 1991-02-27 | 2000-09-04 | シスメックス株式会社 | Flow image cytometer |
JPH0734012B2 (en) * | 1991-02-27 | 1995-04-12 | 東亜医用電子株式会社 | Flow image cytometer |
JP3084296B2 (en) * | 1991-02-27 | 2000-09-04 | シスメックス株式会社 | Flow image cytometer |
JP3070968B2 (en) * | 1991-05-14 | 2000-07-31 | シスメックス株式会社 | Urine cell analysis reagents and methods |
JP3213333B2 (en) * | 1991-05-14 | 2001-10-02 | シスメックス株式会社 | Apparatus and method for analyzing cells in urine. |
JP3213334B2 (en) * | 1991-05-14 | 2001-10-02 | シスメックス株式会社 | Urine cell analyzer |
JP3098273B2 (en) * | 1991-05-14 | 2000-10-16 | シスメックス株式会社 | Urine cell analyzer |
US5412466A (en) * | 1991-07-26 | 1995-05-02 | Toa Medical Electronics Co., Ltd. | Apparatus for forming flattened sample flow for analyzing particles |
US5290701A (en) * | 1991-08-28 | 1994-03-01 | Wilkins Judd R | Microbial detection system and process |
DE69327182T2 (en) * | 1992-02-18 | 2000-06-15 | Hitachi, Ltd. | Device and method for examining particles in a fluid |
US5335293A (en) * | 1992-06-16 | 1994-08-02 | Key Technology, Inc. | Product inspection method and apparatus |
JPH06186156A (en) * | 1992-10-21 | 1994-07-08 | Toa Medical Electronics Co Ltd | Particle analyzer |
JPH06186155A (en) * | 1992-10-21 | 1994-07-08 | Toa Medical Electronics Co Ltd | Particle analyzer |
JP3052665B2 (en) * | 1993-01-26 | 2000-06-19 | 株式会社日立製作所 | Flow cell device |
ATE194431T1 (en) * | 1993-02-26 | 2000-07-15 | E Y Lab Inc | OPTICAL SAMPLE ANALYSIS SYSTEM AND METHOD |
IL106662A (en) * | 1993-08-11 | 1996-10-31 | Yissum Res Dev Co | Flow cell device for monitoring blood or any other cell suspension under flow |
DE69429145T2 (en) * | 1993-08-19 | 2002-07-18 | Hitachi, Ltd. | Classification and test device for particles in a liquid |
JP2826449B2 (en) * | 1993-09-17 | 1998-11-18 | 株式会社日立製作所 | Flow type particle image analysis method and flow type particle image analysis device |
JP3039594B2 (en) * | 1993-10-08 | 2000-05-08 | 株式会社日立製作所 | Staining reagent and method of use |
JPH07120375A (en) * | 1993-10-21 | 1995-05-12 | Hitachi Ltd | Method and apparatus for flow-type particle image analysis |
US5768412A (en) * | 1994-09-19 | 1998-06-16 | Hitachi, Ltd. | Region segmentation method for particle images and apparatus thereof |
AU4741796A (en) * | 1994-12-23 | 1996-07-19 | International Remote Imaging Systems Inc. | Method and apparatus of analyzing particles in a fluid sample and displaying same |
US5625709A (en) * | 1994-12-23 | 1997-04-29 | International Remote Imaging Systems, Inc. | Method and apparatus for identifying characteristics of an object in a field of view |
US5619032A (en) * | 1995-01-18 | 1997-04-08 | International Remote Imaging Systems, Inc. | Method and apparatus for automatically selecting the best focal position from a plurality of focal positions for a focusing apparatus |
JP3189608B2 (en) * | 1995-02-01 | 2001-07-16 | 株式会社日立製作所 | Flow type particle image analysis method |
JPH0991430A (en) * | 1995-09-27 | 1997-04-04 | Hitachi Ltd | Pattern recognition device |
US5983120A (en) * | 1995-10-23 | 1999-11-09 | Cytometrics, Inc. | Method and apparatus for reflected imaging analysis |
US6215892B1 (en) | 1995-11-30 | 2001-04-10 | Chromavision Medical Systems, Inc. | Method and apparatus for automated image analysis of biological specimens |
US6718053B1 (en) * | 1996-11-27 | 2004-04-06 | Chromavision Medical Systems, Inc. | Method and apparatus for automated image analysis of biological specimens |
AU1288497A (en) * | 1995-12-18 | 1997-07-14 | Center For Laboratory Technology, Inc. | Multi-parameter hematology apparatus and method |
JP3587607B2 (en) * | 1995-12-22 | 2004-11-10 | シスメックス株式会社 | Particle measuring apparatus and method |
US5736404A (en) * | 1995-12-27 | 1998-04-07 | Zia Yassinzadeh | Flow detection appartus and method |
US5814468A (en) * | 1996-03-27 | 1998-09-29 | Coulter International Corp. | Methods of enumerating receptor molecules for specific binding partners on formed bodies and in solution |
US6184978B1 (en) | 1996-05-15 | 2001-02-06 | International Remote Imaging Systems, Inc. | Method and apparatus for verifying uniform flow of a fluid sample through a flow cell and distribution on a slide |
US6549661B1 (en) | 1996-12-25 | 2003-04-15 | Hitachi, Ltd. | Pattern recognition apparatus and pattern recognition method |
US6141624A (en) * | 1997-05-13 | 2000-10-31 | International Remote Imaging Systems | Fluid sample for analysis controlled by total fluid volume and by total particle counts |
KR100303608B1 (en) | 1997-05-22 | 2001-11-22 | 박호군 | Method and device for automatically recognizing blood cell |
DE19738626C2 (en) * | 1997-09-04 | 2001-02-08 | Erhard Wendlandt | Micro flow and culture cuvette |
JP2002502980A (en) * | 1998-02-10 | 2002-01-29 | イーワイ ラボラトリーズ インコーポレイテッド | Reflection measurement system that compensates for sample holder undulations and removes system noise lock |
NZ511560A (en) | 1998-11-05 | 2002-11-26 | Chemometec As | A method for the assessment of particles and a system and a device for use in the method |
DE19932870A1 (en) * | 1999-07-09 | 2001-04-05 | Friedrich Schiller Uni Jena Bu | Device for optical particle and particle flow analysis |
US6610973B1 (en) * | 1999-07-27 | 2003-08-26 | Davis, Iii John Merrill | Pill counting aid using a planar light diffusing panel for receipt and retention of the pills |
US6414321B1 (en) | 1999-09-10 | 2002-07-02 | Rudolf Grosskopf | Arrangement for three-dimensional image recording of particles using flow-through systems |
US7236623B2 (en) * | 2000-04-24 | 2007-06-26 | International Remote Imaging Systems, Inc. | Analyte recognition for urinalysis diagnostic system |
PT1301894E (en) | 2000-04-24 | 2009-08-21 | Iris Int Inc | Multi-neural net imaging apparatus and method |
US6473172B1 (en) | 2000-09-20 | 2002-10-29 | International Remote Imaging Systems, Inc. | Flow cell and method of operating therefor |
WO2003042788A2 (en) | 2001-11-13 | 2003-05-22 | Chromavision Medical Systems, Inc. | A system for tracking biological samples |
US20050037406A1 (en) * | 2002-06-12 | 2005-02-17 | De La Torre-Bueno Jose | Methods and apparatus for analysis of a biological specimen |
US7272252B2 (en) * | 2002-06-12 | 2007-09-18 | Clarient, Inc. | Automated system for combining bright field and fluorescent microscopy |
US6873725B2 (en) * | 2002-09-09 | 2005-03-29 | Coulter International Corp. | Simultaneous measurement and display of 3-D size distributions of particulate materials in suspensions |
WO2004046834A1 (en) * | 2002-11-18 | 2004-06-03 | International Remote Imaging Systems, Inc. | A multi-level controller system |
US7150607B2 (en) | 2002-11-18 | 2006-12-19 | International Remote Imaging Systems, Inc. | Uniform flow displacement pump |
EP1565873B1 (en) * | 2002-11-18 | 2013-06-05 | International Remote Imaging Systems, Inc. | Particle extraction for automatic flow microscope |
US7079244B2 (en) * | 2002-11-18 | 2006-07-18 | International Remote Imaging Systems, Inc. | Particle analyzer with specimen tube in-line mixer |
US6825926B2 (en) * | 2002-11-19 | 2004-11-30 | International Remote Imaging Systems, Inc. | Flow cell for urinalysis diagnostic system and method of making same |
US7518716B2 (en) * | 2002-12-20 | 2009-04-14 | J.M. Canty Inc. | Granular product inspection device |
US7245368B2 (en) * | 2003-03-31 | 2007-07-17 | C & L Instruments | Sample chamber for microscopy |
DE10316686A1 (en) * | 2003-04-10 | 2004-10-28 | Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co.KG | Cell for a photometer or a spectrometer |
US20040202357A1 (en) * | 2003-04-11 | 2004-10-14 | Perz Cynthia B. | Silhouette image acquisition |
US7324694B2 (en) * | 2003-05-23 | 2008-01-29 | International Remote Imaging Systems, Inc. | Fluid sample analysis using class weights |
US20050069175A1 (en) * | 2003-09-29 | 2005-03-31 | Tropicana Products, Inc. | Image/optical analysis of citrus pulp |
EP3270156A1 (en) * | 2004-04-07 | 2018-01-17 | Abbott Laboratories | Disposable chamber for analyzing biologic fluids |
US7653260B2 (en) | 2004-06-17 | 2010-01-26 | Carl Zeis MicroImaging GmbH | System and method of registering field of view |
US8582924B2 (en) * | 2004-06-30 | 2013-11-12 | Carl Zeiss Microimaging Gmbh | Data structure of an image storage and retrieval system |
US8121392B2 (en) * | 2004-10-25 | 2012-02-21 | Parata Systems, Llc | Embedded imaging and control system |
US7822276B2 (en) * | 2005-02-17 | 2010-10-26 | Iris International, Inc. | Method and apparatus for analyzing body fluids |
US20070031043A1 (en) * | 2005-08-02 | 2007-02-08 | Perz Cynthia B | System for and method of intelligently directed segmentation analysis for automated microscope systems |
US7731901B2 (en) * | 2005-10-19 | 2010-06-08 | Abbott Laboratories | Apparatus and method for performing counts within a biologic fluid sample |
JP5032792B2 (en) | 2006-05-22 | 2012-09-26 | 浜松ホトニクス株式会社 | Cell sorter |
SE530750C2 (en) * | 2006-07-19 | 2008-09-02 | Hemocue Ab | A measuring device, a method and a computer program |
JP2010501076A (en) * | 2006-08-15 | 2010-01-14 | アメリカ合衆国 | Method and apparatus for attaching a fluid cell to a planar substrate |
US7605919B2 (en) * | 2006-10-30 | 2009-10-20 | Brightwell Technologies Inc. | Method and apparatus for analyzing particles in a fluid |
WO2009073649A1 (en) * | 2007-12-04 | 2009-06-11 | Particle Measuring Systems, Inc. | Non-orthogonal particle detection systems and methods |
EP2255310B1 (en) | 2008-02-29 | 2019-02-20 | Dako Denmark A/S | Systems and methods for tracking and providing workflow information |
US20140152801A1 (en) | 2009-10-28 | 2014-06-05 | Alentic Microscience Inc. | Detecting and Using Light Representative of a Sample |
WO2011053631A1 (en) | 2009-10-28 | 2011-05-05 | Alentic Microscience Inc. | Microscopy imaging |
US9075225B2 (en) | 2009-10-28 | 2015-07-07 | Alentic Microscience Inc. | Microscopy imaging |
US9518920B2 (en) | 2013-06-26 | 2016-12-13 | Alentic Microscience Inc. | Sample processing improvements for microscopy |
US10502666B2 (en) | 2013-02-06 | 2019-12-10 | Alentic Microscience Inc. | Sample processing improvements for quantitative microscopy |
US9857361B2 (en) | 2013-03-15 | 2018-01-02 | Iris International, Inc. | Flowcell, sheath fluid, and autofocus systems and methods for particle analysis in urine samples |
EP2972211B1 (en) | 2013-03-15 | 2018-12-19 | Iris International, Inc. | Flowcell systems and methods for particle analysis in blood samples |
WO2014145983A1 (en) | 2013-03-15 | 2014-09-18 | Iris International, Inc. | Sheath fluid systems and methods for particle analysis in blood samples |
CN103558153B (en) * | 2013-10-31 | 2016-05-25 | 长春迪瑞医疗科技股份有限公司 | Particle imaging chamber and method for designing thereof |
BR112017010964B1 (en) * | 2014-12-22 | 2023-01-31 | Renalsense Ltd | APPARATUS, SYSTEM AND METHODS FOR URINALYSIS |
JP2020054234A (en) | 2017-01-31 | 2020-04-09 | 富士フイルム株式会社 | Cell culture apparatus, imaging unit, and culture monitoring method |
US11181465B2 (en) | 2018-02-01 | 2021-11-23 | Toray Industries, Inc. | Device for evaluating particles in liquid and method for operating same |
CN111684279B (en) * | 2018-08-02 | 2023-02-21 | 深圳迈瑞生物医疗电子股份有限公司 | Cell analysis method, cell analysis device and storage medium |
JP7175158B2 (en) * | 2018-10-29 | 2022-11-18 | アークレイ株式会社 | Information processing device, measurement system, and program |
US10416060B1 (en) | 2019-06-04 | 2019-09-17 | Horiba Instruments Incorporated | Apparatus and method for three-dimensional dynamic image analysis for particle volume determination |
US11125675B2 (en) | 2019-10-18 | 2021-09-21 | Roger Lawrence Deran | Fluid suspended particle classifier |
CN116067852B (en) * | 2022-11-09 | 2023-10-03 | 四川东鹏农海科技有限公司 | Device for measuring suspended pollen particle number and application method thereof |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2791150A (en) * | 1952-02-16 | 1957-05-07 | Daniel S Stevens | Device for determining the red blood cell count |
US3822095A (en) * | 1972-08-14 | 1974-07-02 | Block Engineering | System for differentiating particles |
US4075462A (en) * | 1975-01-08 | 1978-02-21 | William Guy Rowe | Particle analyzer apparatus employing light-sensitive electronic detector array |
US4097845A (en) * | 1976-11-01 | 1978-06-27 | Rush-Presbyterian-St. Luke's Medical Center | Method of and an apparatus for automatic classification of red blood cells |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2480312A (en) * | 1947-02-20 | 1949-08-30 | Glenn C Wolf | Apparatus for the observation and counting of microscopic bodies |
US3390229A (en) * | 1962-11-01 | 1968-06-25 | Raytheon Eduction Company | Particle measuring and counting system |
US3560754A (en) * | 1965-11-17 | 1971-02-02 | Ibm | Photoelectric particle separator using time delay |
US3819270A (en) * | 1972-10-02 | 1974-06-25 | Block Engineering | Blood cell analyzer |
US3924947A (en) * | 1973-10-19 | 1975-12-09 | Coulter Electronics | Apparatus for preservation and identification of particles analyzed by flow-through apparatus |
US3976862A (en) * | 1975-03-18 | 1976-08-24 | Block Engineering, Inc. | Flow stream processor |
US4199748A (en) * | 1976-11-01 | 1980-04-22 | Rush-Presbyterian-St. Luke's Medical Center | Automated method and apparatus for classification of cells with application to the diagnosis of anemia |
DE2656263A1 (en) * | 1976-12-11 | 1978-08-24 | Max Planck Gesellschaft | Measuring device for properties of suspended particles - has medium flow path to measurement orifice which narrows differently in two orthogonal planes |
US4175860A (en) * | 1977-05-31 | 1979-11-27 | Rush-Presbyterian-St. Luke's Medical Center | Dual resolution method and apparatus for use in automated classification of pap smear and other samples |
JPS548312A (en) * | 1977-06-22 | 1979-01-22 | Tokyu Kensetsu Kk | Method of changeeover construction of road* track* etc* to underground line |
-
1980
- 1980-05-02 US US06/146,064 patent/US4338024A/en not_active Expired - Lifetime
-
1981
- 1981-04-09 AU AU71776/81A patent/AU546258B2/en not_active Expired
- 1981-04-09 JP JP56501743A patent/JPH0352573B2/ja not_active Expired - Lifetime
- 1981-04-09 WO PCT/US1981/000479 patent/WO1981003224A1/en active IP Right Grant
- 1981-04-09 GB GB8138017A patent/GB2090427B/en not_active Expired
- 1981-04-09 DE DE813146423T patent/DE3146423T1/en active Granted
- 1981-04-09 EP EP81901339A patent/EP0050666B1/en not_active Expired
- 1981-04-29 CA CA000376531A patent/CA1157157A/en not_active Expired
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2791150A (en) * | 1952-02-16 | 1957-05-07 | Daniel S Stevens | Device for determining the red blood cell count |
US3822095A (en) * | 1972-08-14 | 1974-07-02 | Block Engineering | System for differentiating particles |
US4075462A (en) * | 1975-01-08 | 1978-02-21 | William Guy Rowe | Particle analyzer apparatus employing light-sensitive electronic detector array |
US4097845A (en) * | 1976-11-01 | 1978-06-27 | Rush-Presbyterian-St. Luke's Medical Center | Method of and an apparatus for automatic classification of red blood cells |
Cited By (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2510258A1 (en) * | 1981-07-22 | 1983-01-28 | Int Remote Imaging Systems Inc | PROCESS FOR ANALYZING THE DISTRIBUTION OF A REAGENT IN THE FORM OF PARTICLES AND A LIQUID IN A SUSPENSION |
FR2515352A1 (en) * | 1981-10-22 | 1983-04-29 | Int Remote Imaging Systems Inc | Detecting reagent in immunoassay procedure - by analysing reaction prod. suspension using electrical image processing |
DE3141984A1 (en) * | 1981-10-22 | 1983-05-05 | International Remote Imaging Systems, 91311 Chatsworth, Calif. | Method of analysing for particles |
EP0256867A2 (en) * | 1986-08-15 | 1988-02-24 | Hamilton-Thorn Research | Motility scanner and method |
EP0256867A3 (en) * | 1986-08-15 | 1990-05-16 | Hamilton-Thorn Research | Motility scanner and method |
FR2664983A1 (en) * | 1990-07-17 | 1992-01-24 | Centre Tech Ind Papier | DEVICE FOR THE CONTINUOUS DETECTION OF CONTRAST IMPURITIES CONTAINED IN A MOVING FLUID MATERIAL. |
WO1992001924A1 (en) * | 1990-07-17 | 1992-02-06 | Centre Technique De L'industrie Des Papiers, Cartons Et Celluloses | Device for continuously detecting contrast impurities contained in a moving fluid material |
EP0486747A2 (en) * | 1990-11-22 | 1992-05-27 | Toa Medical Electronics Co., Ltd. | Flow cell mechanism in flow imaging cytometer |
EP0486747A3 (en) * | 1990-11-22 | 1992-10-28 | Toa Medical Electronics Co., Ltd. | Flow cell mechanism in flow imaging cytometer |
WO1993006454A3 (en) * | 1991-09-24 | 1993-05-13 | Thomasset S A Atel | Device for dimensional analysis of particles positioned in a plane |
FR2681693A1 (en) * | 1991-09-24 | 1993-03-26 | Barrat Bertrand | DEVICE FOR DIMENSIONAL ANALYSIS OF PARTICLES POSITIONED IN A PLANE. |
WO1993006454A2 (en) * | 1991-09-24 | 1993-04-01 | Ateliers Thomasset S.A. | Device for dimensional analysis of particles positioned in a plane |
EP0538551A2 (en) * | 1991-10-24 | 1993-04-28 | Toa Medical Electronics Co., Ltd. | Flow imaging cytometer |
EP0538551A3 (en) * | 1991-10-24 | 1993-08-04 | Toa Medical Electronics Co., Ltd. | Flow imaging cytometer |
US5598842A (en) * | 1993-09-03 | 1997-02-04 | Toa Medical Electronics Co., Ltd. | Non-invasive blood analyzer and method using the same |
US5728582A (en) * | 1994-04-21 | 1998-03-17 | Hitachi, Ltd. | Monitoring method of stain solution for particle analysis and calibration method of particle analysis |
EP0678742A3 (en) * | 1994-04-21 | 1996-01-31 | Hitachi Ltd | Monitoring method of stain solution for particle analysis and calibration method of particle analysis. |
EP0678742A2 (en) * | 1994-04-21 | 1995-10-25 | Hitachi, Ltd. | Monitoring method of stain solution for particle analysis and calibration method of particle analysis |
EP0683386A1 (en) * | 1994-05-17 | 1995-11-22 | TOA MEDICAL ELECTRONICS CO., Ltd. | Non-invasive blood analyzer |
DE19919608A1 (en) * | 1999-05-27 | 2000-11-30 | Roche Diagnostics Gmbh | Sample holder for the IR spectroscopy of sample liquids |
US6531702B1 (en) | 1999-05-27 | 2003-03-11 | Roche Diagnostics Gmbh | Sample carrier for the IR spectroscopy of sample liquids |
WO2004099773A1 (en) * | 2003-04-30 | 2004-11-18 | Pfizer Products Inc. | Automated in vitro cellular imaging assays for micronuclei and other target objects |
EP2331992A4 (en) * | 2008-10-02 | 2014-12-17 | Pixcell Medical Technologies Ltd | Optical imaging based on viscoelastic focusing |
EP2397836A1 (en) * | 2010-05-06 | 2011-12-21 | Sony Corporation | Microparticle sorting apparatus, microchip and microchip module |
US8657121B2 (en) | 2010-05-06 | 2014-02-25 | Sony Corporation | Microparticle sorting apparatus, microchip and microchip module |
KR101850548B1 (en) * | 2010-05-06 | 2018-04-19 | 소니 주식회사 | Microparticle sorting apparatus, microchip module, and method of sorting microparticles |
Also Published As
Publication number | Publication date |
---|---|
EP0050666A1 (en) | 1982-05-05 |
EP0050666B1 (en) | 1986-10-01 |
DE3146423C2 (en) | 1993-09-02 |
AU7177681A (en) | 1981-11-26 |
AU546258B2 (en) | 1985-08-22 |
GB2090427B (en) | 1985-01-30 |
CA1157157A (en) | 1983-11-15 |
JPS57500995A (en) | 1982-06-03 |
EP0050666A4 (en) | 1982-09-09 |
US4338024A (en) | 1982-07-06 |
DE3146423T1 (en) | 1982-07-15 |
JPH0352573B2 (en) | 1991-08-12 |
GB2090427A (en) | 1982-07-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU546258B2 (en) | Flow through optical analyzer | |
US4393466A (en) | Method of analyzing particles in a dilute fluid sample | |
GB2122369A (en) | A method of separating particles in a moving fluid sample | |
KR102095617B1 (en) | Autofocus systems and methods for particle analysis in blood samples | |
GB2121976A (en) | A method of aligning particles in a moving fluid sample | |
US4612614A (en) | Method of analyzing particles in a fluid sample | |
Young | The classification of white blood cells | |
CN110226083B (en) | Erythrocyte fragment recognition method and device, blood cell analyzer and analysis method | |
CN113588522A (en) | Circulating tumor detection and sorting method and system based on micro-fluidic and image recognition | |
JPH0341783B2 (en) | ||
CA1164992A (en) | Method of analyzing particles in a dilute fluid sample | |
GB2107548A (en) | Method of analyzing particles in a dilute fluid sample | |
FR2526164A1 (en) | Calibration of optical analyser of particles in blood, urine etc. - using microscopic particles of selected parameter to set standard in computer | |
Eden | Image processing techniques in relation to studies of red cell shape | |
Eden et al. | Feasibility of computer screening of blood films for the detection of malaria parasites | |
CN114414442A (en) | Sample detection method, sample detection device and computer-readable storage medium |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Designated state(s): AU CH DE GB JP SE |
|
AL | Designated countries for regional patents |
Designated state(s): FR |
|
WWE | Wipo information: entry into national phase |
Ref document number: 1981901339 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 1981901339 Country of ref document: EP |
|
RET | De translation (de og part 6b) |
Ref document number: 3146423 Country of ref document: DE Date of ref document: 19820715 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 3146423 Country of ref document: DE |
|
WWG | Wipo information: grant in national office |
Ref document number: 1981901339 Country of ref document: EP |