USPP24973P3 - Corylus plant named ‘Felix’ - Google Patents

Corylus plant named ‘Felix’ Download PDF

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USPP24973P3
USPP24973P3 US13/694,676 US201213694676V USPP24973P3 US PP24973 P3 USPP24973 P3 US PP24973P3 US 201213694676 V US201213694676 V US 201213694676V US PP24973 P3 USPP24973 P3 US PP24973P3
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felix
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corylus
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Shawn A. Mehlenbacher
David C. Smith
Rebecca L. McCluskey
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Oregon State University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy

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  • Botanical denomination Corylus avellana.
  • the present invention relates to a new and distinct cultivar of Corylus plant, (hazelnut, filbert) botanically known as Corylus avellana , and hereinafter referred to by the name ‘Felix’.
  • Corylus avellana is in the family Betulaceae.
  • ‘Felix’ was originally assigned the designation OSU 941.016, which indicates the row and tree location of the original seedling. ‘Delta’ (unpatented) was released by the Oregon Agricultural Experiment Station in 2002. OSU 384.095 (unpatented) is from a cross of ‘Casina’ x OSU 55.129 (both unpatented). OSU 55.129 is from a cross of ‘Tonda Gentile delle Langhe’ (unpatented) ⁇ ‘Extra Ghiaghli’ (unpatented).
  • the pedigree of ‘Felix’ includes ‘Casina’ from Asturias, Spain, ‘Tonda Gentile delle Langhe’ from Piemonte, northern Italy, and ‘Extra Ghiaghli’, which is a clone of the important Turkish cultivar ‘Tombul’ (unpatented).
  • the new cultivar was asexually reproduced by rooted suckers annually for five years (2005-2006 and 2008-2010) in Corvallis, Oreg.
  • the new cultivar was also asexually propagated by whip grafting in Corvallis, Oreg.
  • the unique features of this new Corylus are stable and reproduced true-to-type in successive generations of asexual reproduction.
  • plants of the new Corylus differed from plants of the Corylus avellana cultivar Barcelona (unpatented), and other cultivars and selections of Corylus avellana known to the Inventors primarily in nut size, nut shape, kernel percentage (ratio of kernel weight to nut weight), frequency of blank nuts (nuts lacking kernels), time of pollen shed, time of nut maturity, length of the husk or involucre, and plant size.
  • FIG. 1 shows a tree of the new cultivar ‘Felix’ growing in a field in the summer, in Corvallis, Oreg.
  • FIG. 2 shows the tree of the new cultivar ‘Felix’ growing in a field in January, in Corvallis, Oreg.
  • FIG. 3 shows typical nuts, raw kernels, and blanched kernels of ‘Felix’ hazelnut compared to those of ‘Jefferson’ hazelnut.
  • FIG. 4 shows the husks of ‘Felix’ hazelnut.
  • FIG. 5 shows the typical nuts, raw kernels, and blanched kernels of ‘Felix’ hazelnut compared to those of ‘Barcelona’ hazelnut and other hazelnut cultivars.
  • the cultivar Felix has not been observed under all possible environmental conditions. The phenotype may vary somewhat with variations in environment such as temperature and light intensity, without, however, any variance in genotype.
  • the aforementioned photographs and following observations and measurements describe plants grown in Corvallis, Oreg. under commercial practice outdoors in the field during the fall, winter and spring. Plants used for the photographs and description were propagated by tie-off layerage and growing on their own roots, and about five years old. In the following description, color references are made to The Royal Horticultural Society Colour Chart, 1966 Edition, except where general terms of ordinary dictionary significance are used.
  • GTTTGCTT 2010 (SEQ ID NO: 2) A614 R-GCAGTGGAGGA Gurcan et al. TTGCTGACT 2010 (SEQ ID NO: 4) A616 R-ATGGCTTTTGCT Gurcan et al. TCGTTTTG 2010 (SEQ ID NO: 6) A640 Fam-CGCCATATAATT Gurcan et al. GGGATGCTTGTT 2010 G (SEQ ID NO: 8) B107 R-AACACCATATTG Boccacci et al. AGTCTTTCAAAG 2005; Gokirmak C (SEQ ID NO: 10) et al. 2009 B617 R TGTTTTTGGTGG Gurcan et al.
  • AGCGATG 2010 (SEQ ID NO: 12) B619 R-GCGATCTGACCT Gurcan et al. CATTTTTG 2010 (SEQ ID NO: 14) B634 R-GTGCAGAGGTTG Gurcan et al. CACTCAAA 2010 (SEQ ID NO: 16) B657 R-AGCCTCACCTCC Gurcan et al. AACGAAC 2010 (SEQ ID NO: 18) B671 R-ACCAGCTCTGGG Gurcan et al. CTTAACAC 2010 (SEQ ID NO: 20) B709 R-GCGGGTTCTCGT Gurcan et al. TGTACACT 2010 (SEQ ID NO: 22) B733 R-CATCCCCTGTTG Gurcan et al.
  • GAGTTTTC 2010 (SEQ ID NO: 24) B741 R-CGTGTTGCTCAT Gurcan et al. GTGTTGTG 2010 (SEQ ID NO: 26) B749 R-TCGGCTAGGGTT Gurcan et al. AGGGTTTT 2010 (SEQ ID NO: 28 B751 R-AAACTCAAATA Gurcan et al. AAACCCCTGCTC 2010 (SEQ ID NO: 30) B774 R-TGTGTGGTCT Gurcan et al. GTAGGCACT 2010 (SEQ ID NO: 32) B776 R-TGAGGGGAAGA Gurcan et al. GGTTTGATG 2010 (SEQ ID NO: 34) B795 R-TGGGCATCATCC Gurcan et al.
  • AGGTCTA 2010 (SEQ ID NO: 36) C115 GTTTCCAGATCT Bassil et al. GCCTCCATATAA 2005b, T (SEQ ID NO: Gokirmak et al. 38) 2009 KG809 F-GGAAGGTGAGA Gurcan and GAAATCAAGT Mehlenbacher (SEQ ID NO: 40) 2010 KG811 F-GAACAACTGAA Gurcan and GACAGCAAAG Mehlenbacher (SEQ ID NO: 42) 2010 KG827 GAGGGAGCAAQ Gurcan and TCAAAGTTGAGA Mehlenbacher AGAAA 2010 (SEQ ID NO: 44) KG830 AAAGCAACTCAT Gurcan and AGCTGAAGTCCA Mehlenbacher ATCA 2010 (SEQ ID NO: 46) Soman-G R-GCCATCTTTAGA unpublished AAGTTCGATACAG (SEQ ID NO: 48) Primer fluorescent tags are FAM, HEX, and NED. Ta: annealing temperature (° C.) N: number of alleles

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  • Health & Medical Sciences (AREA)
  • Physiology (AREA)
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  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

A new and distinct cultivar of Corylus plant named ‘Felix’ characterized by an upright plant habit and high vigor, green developing and fully expanded leaves during the spring and summer, resistance to eastern filbert blight caused by the fungus Anisogramma anomala (Peck) E. Wüller, presence of random amplified polymorphic DNA markers 152-800 and AA12-850, expression of incompatibility alleles S15 and S21 in the styles, and DNA fingerprints at 14 of 24 microsatellite marker loci differ from both parents OSU 384.095 and ‘Delta’, and from one parent at an additional 6 marker loci.

Description

ACKNOWLEDGMENT OF GOVERNMENT SUPPORT
This invention was made with government support under Specific Cooperative Agreement No. 58-5358-4542 awarded by the United States Department of Agriculture. The government has certain rights in the invention.
Botanical denomination: Corylus avellana.
Variety designation: ‘Felix’.
BACKGROUND
The present invention relates to a new and distinct cultivar of Corylus plant, (hazelnut, filbert) botanically known as Corylus avellana, and hereinafter referred to by the name ‘Felix’. Corylus avellana is in the family Betulaceae.
The new Corylus resulted from a controlled cross of female parent OSU 384.095 (unpatented) and male parent ‘Delta’ (unpatented) made in 1998 by Shawn A. Mehlenbacher and David C. Smith. Hybrid seeds from the cross were harvested in August 1998, stratified, and seedlings grown in the greenhouse during the summer of 1999. From this cross, total of 157 seedling trees were planted in the field in Corvallis, Oreg., USA in October, 1999. ‘Felix’ was discovered and selected by the Inventors as a single plant within the progeny of the stated cross-pollination in a controlled environment in Corvallis, Oreg. ‘Felix’ was originally assigned the designation OSU 941.016, which indicates the row and tree location of the original seedling. ‘Delta’ (unpatented) was released by the Oregon Agricultural Experiment Station in 2002. OSU 384.095 (unpatented) is from a cross of ‘Casina’ x OSU 55.129 (both unpatented). OSU 55.129 is from a cross of ‘Tonda Gentile delle Langhe’ (unpatented)בExtra Ghiaghli’ (unpatented). The pedigree of ‘Felix’ includes ‘Casina’ from Asturias, Spain, ‘Tonda Gentile delle Langhe’ from Piemonte, northern Italy, and ‘Extra Ghiaghli’, which is a clone of the important Turkish cultivar ‘Tombul’ (unpatented).
The new cultivar was asexually reproduced by rooted suckers annually for five years (2005-2006 and 2008-2010) in Corvallis, Oreg. The new cultivar was also asexually propagated by whip grafting in Corvallis, Oreg. The unique features of this new Corylus are stable and reproduced true-to-type in successive generations of asexual reproduction.
SUMMARY
The following traits have been repeatedly observed and are determined to be the unique characteristics of ‘Felix’. These characteristics in combination distinguish ‘Felix’ as a new and distinct cultivar:
    • 1. Upright plant habit and high vigor.
    • 2. Green developing and fully expanded leaves during the spring and summer.
    • 3. Resistance to eastern filbert blight caused by the fungus Anisogramma anomala (Peck) E. Müller.
    • 4. Presence of random amplified polymorphic DNA markers 152-800 and AA12-850 in DNA of ‘Felix’ amplified by the polymerase chain reaction. These two markers are linked to a dominant allele for resistance to eastern filbert blight from the cultivar Gasaway (unpatented).
    • 5. Expression of incompatibility alleles S15 and S21 in the styles.
    • 6. DNA fingerprints at 14 of 24 microsatellite marker loci differ from both parents OSU 384.095 and ‘Delta’, and from one parent at an additional 6 marker loci. DNA fingerprints of standard cultivars ‘Barcelona’, ‘Tonda Gentile delle Langhe’ and ‘Extra Ghiaghli’, and ‘Gasaway’, the source of eastern filbert blight resistance, are also shown in the attached table.
In comparisons in two replicated trials conducted in Corvallis, Oreg., plants of the new Corylus differed from plants of the Corylus avellana cultivar Barcelona (unpatented), and other cultivars and selections of Corylus avellana known to the Inventors primarily in nut size, nut shape, kernel percentage (ratio of kernel weight to nut weight), frequency of blank nuts (nuts lacking kernels), time of pollen shed, time of nut maturity, length of the husk or involucre, and plant size.
BRIEF DESCRIPTION OF THE DRAWINGS
The accompanying colored photographs illustrate the overall appearance of the new cultivar, showing the colors as true as it is reasonably possible to obtain in colored reproductions of this type. Foliage colors in the photographs may differ slightly from the color values cited in the detailed botanical description which accurately describe the colors of the new Corylus.
FIG. 1 shows a tree of the new cultivar ‘Felix’ growing in a field in the summer, in Corvallis, Oreg.
FIG. 2 shows the tree of the new cultivar ‘Felix’ growing in a field in January, in Corvallis, Oreg.
FIG. 3 shows typical nuts, raw kernels, and blanched kernels of ‘Felix’ hazelnut compared to those of ‘Jefferson’ hazelnut.
FIG. 4 shows the husks of ‘Felix’ hazelnut.
FIG. 5 shows the typical nuts, raw kernels, and blanched kernels of ‘Felix’ hazelnut compared to those of ‘Barcelona’ hazelnut and other hazelnut cultivars.
DETAILED PLANT DESCRIPTION
The cultivar Felix has not been observed under all possible environmental conditions. The phenotype may vary somewhat with variations in environment such as temperature and light intensity, without, however, any variance in genotype. The aforementioned photographs and following observations and measurements describe plants grown in Corvallis, Oreg. under commercial practice outdoors in the field during the fall, winter and spring. Plants used for the photographs and description were propagated by tie-off layerage and growing on their own roots, and about five years old. In the following description, color references are made to The Royal Horticultural Society Colour Chart, 1966 Edition, except where general terms of ordinary dictionary significance are used.
  • Botanical classification: Corylus avellana cultivar Felix.
  • Parentage:
      • Female, or seed, parent.—Corylus avellana selection OSU 384.095 (unpatented).
      • Male, or pollen, parent.—Corylus avellana cultivar ‘Delta’ (unpatented).
  • Propagation (type rooted suckers):
      • Time to initiate roots.—About 30 days at 20° C.
      • Time to produce a rooted young plant.—About six months at 22° C.
      • Root description.—Fine to thick; freely branching; creamy white in color.
  • Propagation (type whip grafting):
      • Time to budbreak on the scions.—About 14 days at 25° C.
      • Time to produce a grafted plant.—About six months at 25° C.
  • Plant description:
      • Type.—Perennial shrub. Upright plant habit.
      • Growth and branching .—Freely branching; about 15 lateral branches develop per plant. Pinching, i.e., removal of the terminal apices, enhances branching with lateral branches potentially forming at every node.
      • Size.—Plant height is about 6 meters; plant diameter or spread is about 5 meters.
      • Vigor.—Vigorous growth.
      • Lenticels.—6 circular within 1 square centimeter (counted on dormant scions).
  • Lateral branch description:
      • Length.—About 43 cm.
      • Diameter.—About 6 mm.
      • Internode length.—About 2.8 cm.
      • Texture.—Smooth, glabrous.
      • Strength.—Strong.
      • Color.—Immature — 152B; mature — 152B.
  • Foliage description:
      • Arrangement.—Alternate, simple.
      • Length.—About 10.6 cm.
      • Width.—About 10.1 cm.
      • Shape.—Oblong to ovate.
      • Apex.—Obtuse to acute.
      • Base.—Cordate.
      • Margin.—Serrate.
      • Texture, upper and lower surfaces.—Slightly pubescent.
      • Venation pattern.—Pinnate.
      • Leaf bud shape.—Ovid.
      • Time of leaf bud burst.—Late, 15 days after ‘Barcelona’.
      • Color.—Developing foliage, upper surface 144A, lower surfaces: 145A. Fully expanded foliage, upper surface: Spring and summer, 143A; late summer and fall, 143A. Fully expanded foliage, lower surface: Spring and summer, 139C; late summer and fall, 139C. Venation, upper surface: Spring and summer, 139C; late summer and fall, 139C. Venation, lower surface: Spring and summer, 139D; late summer and fall, 139D. Leaf bud, 178C.
  • Petiole description:
      • Length.—About 2.7 cm.
      • Diameter.—About 1.8 mm.
      • Texture.—Upper and lower surfaces — pubescent.
      • Color.—Upper surface: Spring and summer, 139D; late summer and fall, 139D. lower surface: Spring and summer, 139D; late summer and fall, 139D.
  • Flower description:
      • Male inflorescences.—Catkins, color prior to elongation 194C in shade, 176D where exposed to sun.
      • Female inflorescence.—Style color 047B.
      • Stigma coloration.—047B.
      • Time of female flowering.—Late, 2.5 weeks after ‘Barcelona’.
      • Time of pollen shed.—Late midseason, around the same time as ‘Hall's Giant’ (unpatented).
  • Involcure description:
      • Involucre constriction.—Absent.
      • Involucre length.—About 60% longer than nuts.
      • Strength of serration of indentation.—Deeply serrated.
      • Pubescence.—Little.
      • Thickness of callus at base.—Moderate callus at base similar to ‘Barcelona’.
      • Description of jointing of bracts.—About 75% of involucre slit to the base on one side, and about 25% are entire and tubular. Involucre adheres to a few of the nuts after drop, at the side of the basal scar. About 15% are loosely held in tubular husks when the nuts fall.
  • Nut description:
      • Length.—About 18.7 mm.
      • Width.—About 18.9 mm.
      • Depth.—About 16.7 mm.
      • Nut shape.—Round.
      • Nut shape index [(width+depth)/2*length].—0.95.
      • Nut compression index (width/depth).—1.13.
      • Nut shell color.—167A.
      • Nut weight.—About 2.65 grams to 2.74 grams.
      • Predominant number of fruits per cluster.—Average 2 nuts per cluster.
      • Stripes on shell.—About 30, color slightly darker than 167A.
      • Fruit apex.—Moderately prominent.
      • Size of the fruit pistil scar.—Very small (0.5 mm×1 mm).
      • Nut curvature of the basal scar.—Flat (plane).
      • Frequency of blank nuts.—4.2%.
      • Time of nut maturity.—About 4 days earlier that ‘Barcelona’.
      • Husk length.—About 50% longer than the nuts.
      • Kernel weight.—About 1.32 grams to 1.37 grams.
      • Kernel percentage (kernel weight/nut weight).—About 50%.
      • Kernel shape.—Globular.
      • Kernel cross section shape.—Circular.
      • Kernel base shape.—Flat.
      • Lateral grooves.—None.
  • Disease/pest resistance: Plants of the new Corylus are highly resistant to eastern filbert blight caused by the fungus Anisogramma anomala (Peck) E. Müller. Plants of the new Corylus are moderately resistant to bud mites (Phytoptus avellanae Nal.), while plants of ‘Tonda Gentile delle Langhe’ are highly susceptible, and plants of ‘Barcelona’ are highly resistant.
  • Temperature tolerance: Tolerates temperatures from −10 to 38° C. in the field in Corvallis, Oreg.
TABLE 1
Primers and annealing temperatures for the 24
microsatellite marker loci used to
fingerprint ‘Felix’ and other hazelnut cultivars.
Repeat
Locus motif Size Ta n He Ho
A613 (TC)13(CA)12 149-177 60 14 0.85 0.85
A614 (TC)17(CA)10 125-156 60 14 0.85 0.85
NNN(CA)6
A616 (AC)11 136-162 60 13 0.85 0.85
A640 (CT)15 354-378 67 11 0.80 0.73
(CA)13
B107 (CT)14 112-151 55 14 0.85 0.80
B617 (GA)15 280-298 60 9 0.80 0.78
B619 (TC)21 146-180 60 14 0.88 0.88
B634 (AG)15 218-238 60 9 0.76 0.76
B657 (AG)15 210-228 60 8 0.84 0.98
B671 (AG)6NN 221-249 60 13 0.86 0.88
(GA)17
B709 (GA)21 219-233 60 8 0.74 0.76
B733 (TC)15 161-183 60 8 0.68 0.68
B741 (GT)5(GA)12 176-194 60 10 0.77 0.78
B749 (TC)12 200-210 60 6 0.60 0.64
B751 (GA)15 141-153 60 7 0.80 0.80
B774 (AG)15 195-213 60 8 0.80 0.80
B776 (GA)17 134-148 60 7 0.71 0.60
B795 (TC)8Ns(CT)7 296-332 60 12 0.76 0.74
Ns(CT)10
Ns(TC)5
C115 (TAA)5 167-226 60 14 0.80 0.80
(GAA)12
KG809 (AGG)6 333-345 55 5 0.66 0.64
KG811 (GA)17 240-278 58 12 0.83 0.82
KG827 (CT)13AA 264-282 67 9 0.78 0.84
(CA)7
KG830 (CT)14 279-311 67 9 0.79 0.78
GTATT
(CA)8
Soman- (AAT)5 54 3 0.60 0.98
G
Locus PIC r  LG Primers 5′-3′
A613 0.85 0.00 11 Ned-CACACGCCTT
GTCACTCTTT
(SEQ ID NO: 1)
A614 0.84 0.00 6 Hex-TGGCAGAGCT
TTGTCAGCTT
(SEQ ID NO: 3)
A616 0.83 0.00 8 Fam-CACTCATACC
GCAAACTCCA
(SEQ ID NO: 5)
A640 0.7 0.04 10 F-TGCCTCTGCA
GTTAGTCATC
AAATGTAGG
(SEQ ID NO: 7)
B107 0.83 0.02 10 Ned-GTAGGTGCAC
TTGATGTGCTT
TAC (SEQ ID NO: 9)
B617 0.78 0.01 8 Fam-TCCGTGTTGA
GTATGGACGA
(SEQ ID NO: 11)
B619 0.7 0.00 3 Fam-AGTCGGCTCC
CCTTTTCTC
(SEQ ID NO: 13)
B634 0.73 0.00 4 Hex-CCTGCATCCA
GGACTCATTA 60
(SEQ ID NO: 15)
B657 0.82 −0.08 11 Ned-GAGAGTGCGT
CTTCCTCTGG
(SEQ ID NO: 17)
B671 0.84 −0.01 9 Hex-TTGCCAGTGC
ATACTCTGAT G
(SEQ ID NO: 19)
B709 0.70 −0.01 5 Ned-CCAAGCACGA
ATGAACTCAA
(SEQ ID NO: 21)
B733 0.63 0.00 7.2 Ned-CACCCTCTTCA
CCACCTCAT
(SEQ ID NO: 23)
B741 0.74 0.00 5 Fam-GTTCACAGGC
TGTTGGGTTT
(SEQ ID NO: 25)
B749 0.51 −0.03 1 Hex-GGCTGACAAC
ACAGCAGAAA
(SEQ ID NO: 27)
B751 0.77 0.01 7.2 Fam-AGCTGGTTCTT
CGACATTCC
(SEQ ID NO: 29)
B774 0.77 0.01 5 Ned-GTTTTGCGAG
CTCATTGTCA
(SEQ ID NO: 31)
B776 0.67 0.07 6 Fam-TGTATGTACA
CACGGAGAGA
GAGA (SEQ
ID NO: 33)
B795 0.74 0.01 NA Fam-GACCCACAAA
CAATAACCTA
TCTC (SEQ
ID NO: 35)
C115 0.77 0.00 4 Fam-ATTTTCCGCA
GATAATACAGG
(SEQ ID NO: 37)
KG809 0.60 0.01 4 Hex-AGGCATCAGT
TCATCCAA
(SEQ ID NO: 39)
KG811 0.81 0.01 2 Ned-AAGGCGGCAC
TCGCTCAC
(SEQ ID NO: 41)
KG827 0.75 −0.04 9 Fam-AGAACTCCGA
CTAATAATCC
TAACCCTTGC
(SEQ ID NO: 43)
KG830 0.76 0.00 9 Ned-TGGAGGAAGT
TTTGAATGGT
AGTAGAGGA
(SEQ ID NO: 45)
Soman-G 0.51 −0.27 NA Hex-TGGCGTTGCA
ACATATTCTC
(SEQ ID NO: 47)
Locus Primers 5′-3′ Reference
A613 R-CCCCTTTCACAT Gurcan et al.
GTTTGCTT 2010
(SEQ ID NO: 2)
A614 R-GCAGTGGAGGA Gurcan et al.
TTGCTGACT 2010
(SEQ ID NO: 4)
A616 R-ATGGCTTTTGCT Gurcan et al.
TCGTTTTG  2010
(SEQ ID NO: 6)
A640 Fam-CGCCATATAATT Gurcan et al.
GGGATGCTTGTT  2010
G (SEQ ID NO: 8)
B107 R-AACACCATATTG Boccacci et al.
AGTCTTTCAAAG 2005; Gokirmak
C (SEQ ID NO: 10) et al. 2009
B617 R TGTTTTTGGTGG Gurcan et al.
AGCGATG 2010
(SEQ ID NO: 12)
B619 R-GCGATCTGACCT Gurcan et al.
CATTTTTG 2010
(SEQ ID NO: 14)
B634 R-GTGCAGAGGTTG Gurcan et al.
CACTCAAA 2010
(SEQ ID NO: 16)
B657 R-AGCCTCACCTCC Gurcan et al.
AACGAAC 2010
(SEQ ID NO: 18)
B671 R-ACCAGCTCTGGG Gurcan et al.
CTTAACAC 2010
(SEQ ID NO: 20)
B709 R-GCGGGTTCTCGT Gurcan et al.
TGTACACT 2010
(SEQ ID NO: 22)
B733 R-CATCCCCTGTTG Gurcan et al.
GAGTTTTC 2010
(SEQ ID NO: 24)
B741 R-CGTGTTGCTCAT Gurcan et al.
GTGTTGTG 2010
(SEQ ID NO: 26)
B749 R-TCGGCTAGGGTT Gurcan et al.
AGGGTTTT 2010
(SEQ ID NO: 28
B751 R-AAACTCAAATA Gurcan et al.
AAACCCCTGCTC 2010
(SEQ ID NO: 30)
B774 R-TGTGTGTGGTCT Gurcan et al.
GTAGGCACT 2010
(SEQ ID NO: 32)
B776 R-TGAGGGGAAGA Gurcan et al.
GGTTTGATG 2010
(SEQ ID NO: 34)
B795 R-TGGGCATCATCC Gurcan et al.
AGGTCTA 2010
(SEQ ID NO: 36)
C115 GTTTCCAGATCT Bassil et al.
GCCTCCATATAA 2005b, 
T (SEQ ID NO: Gokirmak et al.
38) 2009
KG809 F-GGAAGGTGAGA Gurcan and
GAAATCAAGT Mehlenbacher
(SEQ ID NO: 40) 2010
KG811 F-GAACAACTGAA Gurcan and
GACAGCAAAG Mehlenbacher
(SEQ ID NO: 42) 2010
KG827 GAGGGAGCAAQ Gurcan and
TCAAAGTTGAGA Mehlenbacher
AGAAA 2010
(SEQ ID NO: 44)
KG830 AAAGCAACTCAT Gurcan and
AGCTGAAGTCCA Mehlenbacher
ATCA 2010
(SEQ ID NO: 46)
Soman-G R-GCCATCTTTAGA unpublished
AAGTTCGATACAG
(SEQ ID NO: 48)
Primer fluorescent tags are FAM, HEX, and NED.
Ta: annealing temperature (° C.)
N: number of alleles
He: expected heterozygosity
Ho: observed heterozygosity
PIC: polymorphism information content
r: estimated null allele frequency
LG: linkage group
TABLE 2
Allele sizes in ‘Felix’ and
other hazelnut cultivars at 24 microsatellite loci.
‘Tonda Gentile
Tag Locus ‘Felix’ ‘384.095’ ‘Delta’ delleLanghe’
NED A613 149/151 151/169 149/177 151/157
HEX A614 139/143 135/139 143/158 125/135
FAM A616 150/150 150/150 150/150 148/150
FAM A640 368/372 368/372 362/372 354/368
NED B107 130/152 130/152 122/130 134/152
FAM B617 286/286 286/286 286/286 286/296
FAM B619 156/164 148/164 156/164 148/164
HEX B634 226/234 226/226 226/234 226/226
NED B657 218/226 218/222 222/226 218/226
HEX B671 227/235 227/247 235/247 237/241
NED B709 227/231 225/231 227/227 227/227
NED B733 173/179 171/173 173/179 171/173
FAM B741 177/186 177/186 177/186 177/184
HEX B749 206/206 206/208 206/208 206/208
FAM B751 151/153 143/153 143/151 149/153
NED B774 203/213 203/203 207/213 203/211
FAM B776 148/150 137/148 137/150 137/137
FAM B795 330/330 310/330 314/330 312/330
FAM C115 197/215 173/197 197/215 173/173
HEX KG809 336/345 336/336 345/345 336/339
NED KG811 248/264 242/248 254/264 254/264
FAM KG827 270/282 276/282 270/270 266/268
NED KG830 291/303 289/303 291/297 291/295
HEX SMNG 196/200 196/200 196/196 196/200
‘Extra
Tag Locus ‘Barcelona’ Ghiaghli’ ‘Gasaway’
NED A613 151/159 167/169 159/161
HEX A614 125/131 125/150 143/158
FAM A616 142/150 150/158 148/148
FAM A640 354/374 374/374 362/368
NED B107 112/134 116/116 122/128
FAM B617 286/290 294/296 292/296
FAM B619 156/170 164/174 170/174
HEX B634 226/226 226/226 220/232
NED B657 218/222 210/222 224/228
HEX B671 223/227 227/247 235/247
NED B709 225/233 225/227 227/227
NED B733 171/173 171/171 173/173
FAM B741 177/186 177/184 186/188
HEX B749 208/208 208/208 206/208
FAM B751 143/153 143/147 143/143
NED B774 203/207 195/203 203/209
FAM B776 135/137 135/137 146/150
FAM B795 330/330 296/310 314/316
FAM C115 173/194 182/194 215/218
HEX KG809 336/336 336/339 336/345
NED KG811 258/264 240/242 254/258
FAM KG827 280/282 276/282 270/280
NED KG830 291/295 291/295 291/305
HEX SMNG 196/200 196/200 196/196

References:
  • Bassil N. V., Botta R., Mehlenbacher S. A. 2005a. Microsatellite markers in hazelnut: Isolation, characterization and cross-species amplification. J. Amer. Soc. Hort. Sci. 130:543-549.
  • Bassil N. V., Botta R., Mehlenbacher S. A. 2005b. Additional microsatellite markers of the European hazelnut. Acta Hort. 686:105-110.
  • Boccacci P., Akkak A., Bassil N. V., Mehlenbacher S. A., Botta R. 2005. Characterization and evaluation of microsatellite loci in European hazelnut (C. avellana) and their transferability to other Corylus species. Molec. Ecol. Notes 5:934-937.
  • Boccacci P., Akkak, A. and Botta, R. 2006. DNA typing and genetic relations among European hazelnut (Corylus avellana L.) cultivars using microsatellite markers. Genome 49:598-611.
  • Gökirmak T., Mehlenbacher S. A., Bassil N. V. 2009. Characterization of European hazelnut (Corylus avellana) cultivars using SSR markers. Genetic Resources and Crop Evolution 56:147-172.
  • Gürcan, K., S. A. Mehlenbacher and V. Erdogan. 2010a. Genetic diversity in hazelnut cultivars from Black Sea countries assessed using SSR markers. Plant Breeding (available on-line doi:10.1111/j.1439-0523.2009.01753.x).
  • Gürcan, K., S. A. Mehlenbacher, N. V. Bassil, P. Boccacci, A. Akkak and R. Botta. 2010b. New microsatellite markers for Corylus avellana from enriched libraries. Tree Genetics and Genomes (available on-line as DOI10.1007/s11295-010-0269-y).
  • Gürcan, K. and S. A. Mehlenbacher. 2010. Development of microsatellite marker loci for European hazelnut (Corylus avellana L.) from ISSR fragments. Molecular Breeding (available on-line).

Claims (1)

We claim:
1. A new and distinct cultivar of Corylus plant named ‘Felix’, as illustrated and described.
US13/694,676 2012-12-24 2012-12-24 Corylus plant named ‘Felix’ Active 2033-02-27 USPP24973P3 (en)

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