USPP22715P2 - Corylus plant named ‘Tonda Pacifica’ - Google Patents
Corylus plant named ‘Tonda Pacifica’ Download PDFInfo
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- USPP22715P2 USPP22715P2 US12/928,688 US92868810V USPP22715P2 US PP22715 P2 USPP22715 P2 US PP22715P2 US 92868810 V US92868810 V US 92868810V US PP22715 P2 USPP22715 P2 US PP22715P2
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- 241000723382 Corylus Species 0.000 title claims abstract description 19
- 241000196324 Embryophyta Species 0.000 description 22
- 235000007466 Corylus avellana Nutrition 0.000 description 14
- 108091092878 Microsatellite Proteins 0.000 description 9
- 108700028369 Alleles Proteins 0.000 description 7
- 235000001543 Corylus americana Nutrition 0.000 description 7
- 240000007582 Corylus avellana Species 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 5
- 108020004414 DNA Proteins 0.000 description 4
- 239000010903 husk Substances 0.000 description 3
- 239000003550 marker Substances 0.000 description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 3
- YRIZYWQGELRKNT-UHFFFAOYSA-N 1,3,5-trichloro-1,3,5-triazinane-2,4,6-trione Chemical compound ClN1C(=O)N(Cl)C(=O)N(Cl)C1=O YRIZYWQGELRKNT-UHFFFAOYSA-N 0.000 description 2
- BCOSEZGCLGPUSL-UHFFFAOYSA-N 2,3,3-trichloroprop-2-enoyl chloride Chemical compound ClC(Cl)=C(Cl)C(Cl)=O BCOSEZGCLGPUSL-UHFFFAOYSA-N 0.000 description 2
- 241000238876 Acari Species 0.000 description 2
- 241001110470 Anisogramma anomala Species 0.000 description 2
- 241000252254 Catostomidae Species 0.000 description 2
- 241000627733 Phytoptus avellanae Species 0.000 description 2
- 238000000137 annealing Methods 0.000 description 2
- 238000005251 capillar electrophoresis Methods 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 241000023308 Acca Species 0.000 description 1
- 101000869690 Homo sapiens Protein S100-A8 Proteins 0.000 description 1
- FSNCEEGOMTYXKY-JTQLQIEISA-N Lycoperodine 1 Natural products N1C2=CC=CC=C2C2=C1CN[C@H](C(=O)O)C2 FSNCEEGOMTYXKY-JTQLQIEISA-N 0.000 description 1
- 206010033546 Pallor Diseases 0.000 description 1
- 102100032442 Protein S100-A8 Human genes 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000011681 asexual reproduction Effects 0.000 description 1
- 238000013465 asexual reproduction Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 230000010154 cross-pollination Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 230000033458 reproduction Effects 0.000 description 1
- 238000004513 sizing Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/08—Fruits
Definitions
- Corylus avellana Latin name of the genus and species of the plant claimed: Corylus avellana.
- the present invention relates to a new and distinct cultivar of Corylus plant (hazelnut, filbert), botanically known as Corylus avellana , and hereinafter referred to by the name ‘Tonda Pacifica.’
- ‘Tonda Gentile delle Langhe’ is an important cultivar in Piemonte, northern Italy.
- the male parent, OSU 23.024, is from a controlled cross of ‘Barcelona’ and ‘Extra Ghiaghli’.
- ‘Extra Ghiaghli’, obtained as scions from Greece, is a clone of the important Turkish cultivar ‘Tombul’.
- the new cultivar was asexually reproduced by rooted suckers annually for seven years in Corvallis, Oreg.
- the new cultivar was also asexually propagated by whip grafting in Corvallis, Oreg.
- the unique features of this new Corylus are stable and reproduce true-to-type in successive generations of asexual reproduction.
- ‘Tonda Pacifica’ combines the high kernel quality of its parent ‘Tonda Gentile delle Langhe’ with higher yields, thinner shells, and lower susceptibility to bud mites (primarily Phytoptus avellanae Nal.).
- the photograph on the first sheet comprises a side perspective view of a typical plant of ‘Tonda Pacifica’ in Corvallis, Oreg.
- the photograph on the second sheet shows typical nuts, raw kernels, and blanched kernels of ‘Tonda Pacifica’.
- the cultivar ‘Tonda Pacifica’ has not been observed under all possible environmental conditions. The phenotype may vary somewhat with variations in environment such as temperature and light intensity, without, however, any variance in genotype.
- the aforementioned photographs and following observations and measurements describe plants grown in Corvallis, Oreg. under commercial practice outdoors in the field during the fall, winter, and spring. Plants used for the photographs and description were about twelve years old. In the following description, color references are made to The Royal Horticultural Society Colour Chart, 1966 Edition, except where general terms of ordinary dictionary significance are used. The following description is based on self-rooted trees growing in Corvallis, Oreg. For grafting, the rootstocks were cv. ‘Montebello’, not patented, propagated by tie-off layerage (stooling).
- Nut yields of ‘Tonda Pacifica’ are similar or slightly higher than nut yields for ‘Barcelona’. Since ‘Tonda Pacifica’ trees are smaller than ‘Barcelona’ trees, the nut yield efficiency for ‘Tonda Pacifica’ is significantly higher than that for ‘Barcelona’.
- the nuts of ‘Tonda Pacifica’ are borne in clusters of 3-4 in husks 75 to 100% longer than the nuts. The husks are slit down the side and the nuts fall free at maturity.
- the harvest date for the nuts of ‘Tonda Pacifica’ is estimated to be 7-10 days before ‘Barcelona’. ‘Tonda Pacifica’ produces fewer blank nuts and fewer defects than ‘Barcelona’.
- Raw kernels have a moderate amount of fiber attached to the pellicle, which can be mostly removed by light roasting and rubbing. Blanching scores for ‘Tonda Pacifica’ are better than for ‘Barcelona’ and ‘Tonda Gentile delle Langhe’.
- SSR simple sequence repeat
- Twenty nine marker loci were selected from those published for hazelnut (Bassil et al. (2005) J. Amer. Soc. Hort. Sci., 130:543-549; Bassil et al. (2005) Acta Hort., 686:105-110; Boccacci et al. (2005) Molec. Ecol. Notes, 5:934-937; Gürcan et al. (2010) Tree Genetics and Genomes (available on-line as DOI 10.1007/s11295-010-0269-y); Gürcan et al. (2010) Molecular Breeding, 26:551-559).
- SSR markers have been used for fingerprinting hazelnut accessions (Boccacci et al. (2006) Genome 49:598-611; Gökirmak et al. (2009) Genetic Resources and Crop Evolution, 56:147-172; Gürcan et al. (2010) Plant Breeding, 129:422-434).
- hazelnut DNA was amplified by PCR.
- One of the two primers was fluorescently labeled with FAM, HEX, or NED, and the size of the amplified fragment was determined by capillary electrophoresis on an ABI 3100 instrument (Applied Biosystems; Foster City, Calif.).
- ‘Tonda Pacifica’ was found to exhibit different allele sizes at certain loci that allowed for it to be distinguish from other hazelnut genotypes such as its parent ‘Tonda Gentile delle Langhe’ and its grandparents ‘Barcelona’ and ‘Extra Ghiaghli’ (Table 2).
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
A new and distinct cultivar of Corylus plant named ‘Tonda Pacifica’ is provided.
Description
Latin name of the genus and species of the plant claimed: Corylus avellana.
Variety denomination: ‘Tonda Pacifica’.
The present invention relates to a new and distinct cultivar of Corylus plant (hazelnut, filbert), botanically known as Corylus avellana, and hereinafter referred to by the name ‘Tonda Pacifica.’
The new Corylus resulted from a controlled cross of female parent ‘Tonda Gentile delle Langhe’ and male parent OSU 23.024 made in 1981 by Maxine M. Thompson. Neither parent was protected by a plant patent. Hybrid seeds from the cross were harvested in August 1981, stratified, and seedlings grown in the greenhouse during the summer of 1982. From this cross, total of 95 seedling trees were planted in the field in Corvallis in October, 1982. ‘Tonda Pacifica’ was discovered and selected as a single plant within the progeny of the stated cross-pollination in a controlled environment in Corvallis, Oreg., USA. It was originally assigned the designation OSU 228.084, which indicates the row and tree location of the original seedling. ‘Tonda Gentile delle Langhe’ is an important cultivar in Piemonte, northern Italy. The male parent, OSU 23.024, is from a controlled cross of ‘Barcelona’ and ‘Extra Ghiaghli’. ‘Extra Ghiaghli’, obtained as scions from Greece, is a clone of the important Turkish cultivar ‘Tombul’.
The new cultivar was asexually reproduced by rooted suckers annually for seven years in Corvallis, Oreg. The new cultivar was also asexually propagated by whip grafting in Corvallis, Oreg. The unique features of this new Corylus are stable and reproduce true-to-type in successive generations of asexual reproduction.
The following traits have been observed and are determined to be the unique characteristics of ‘Tonda Pacifica.’ These characteristics in combination distinguish ‘Tonda Pacifica’ as a new and distinct cultivar:
-
- 1. Globose plant habit.
- 2. Yellowish-green developing and fully expanded leaves during the spring and summer.
- 3. Susceptibility to eastern filbert blight caused by the fungus Anisogramma anomala (Peck) E. Müller.
- 4. Absence of random amplified polymorphic DNA markers 152-800 and 268-580 in DNA of ‘Tonda Pacifica’ amplified by the polymerase chain reaction (PCR). These two markers are linked to a dominant allele for resistance to eastern filbert blight from the cultivar Gasaway, not patented.
- 5. Expression of incompatibility alleles S1 and S2 in the styles.
- 6. DNA fingerprints at 28 microsatellite marker loci differ from its parent ‘Tonda Gentile delle Langhe’ and grandparents ‘Barcelona’ and ‘Extra Ghiaghli’. The microsatellite primers are shown in Table 1, and allele sizes are shown in Table 2.
Comparisons in three replicated trials conducted in Corvallis, Oreg., plants of the new Corylus differed from plants of the Corylus avellena cultivar ‘Tonda Gentile delle Langhe’, not patented, and other cultivars and selections of Corylus avellena known to the Inventors primarily in nut size, nut shape, kernel percentage (ratio of kernel weight to nut weight), frequency of blank nuts (nuts lacking kernels), time of pollen shed, time of nut maturity, length of the husk or involucre, and plant size. ‘Tonda Pacifica’ combines the high kernel quality of its parent ‘Tonda Gentile delle Langhe’ with higher yields, thinner shells, and lower susceptibility to bud mites (primarily Phytoptus avellanae Nal.).
The accompanying colored photographs illustrate the overall appearance of the new cultivar, showing the colors as true as it is reasonably possible to obtain in colored reproductions of this type.
The photograph on the first sheet comprises a side perspective view of a typical plant of ‘Tonda Pacifica’ in Corvallis, Oreg.
The photograph on the second sheet shows typical nuts, raw kernels, and blanched kernels of ‘Tonda Pacifica’.
The cultivar ‘Tonda Pacifica’ has not been observed under all possible environmental conditions. The phenotype may vary somewhat with variations in environment such as temperature and light intensity, without, however, any variance in genotype. The aforementioned photographs and following observations and measurements describe plants grown in Corvallis, Oreg. under commercial practice outdoors in the field during the fall, winter, and spring. Plants used for the photographs and description were about twelve years old. In the following description, color references are made to The Royal Horticultural Society Colour Chart, 1966 Edition, except where general terms of ordinary dictionary significance are used. The following description is based on self-rooted trees growing in Corvallis, Oreg. For grafting, the rootstocks were cv. ‘Montebello’, not patented, propagated by tie-off layerage (stooling).
- Botanical classification: Corylus avellana cultivar ‘Tonda Pacifica.’
- Parentage:
-
- Female, or seed, parent.—Corylus avellana ‘Tonda Gentile delle Langhe’, not patented.
- Male, or pollen, parent.—Corylus avellana selection OSU 23.024, not patented.
-
- Propagation:
-
- Type.—Rooted suckers.
- Time to initiate roots.—About 30 days at 20° C.
- Time to produce a rooted young plant.—About six months at 22° C.
- Root description.—Fine to thick; freely branching; creamy white in color.
- Type.—Whip grafting.
- Time to budbreak on the scions.—About 14 days at 25° C.
- Time to produce a grafted plant.—About six months at 25° C.
-
- Plant description:
-
- General appearance.—Perennial shrub. Globose plant habit, with slightly pendulous shoots.
- Growth and branching habit.—Freely branching; about 15 lateral branches develop per plant. Pinching, i.e., removal of the terminal apices, enhances branching with lateral branches potentially forming at every node. Moderately vigorous growth habit.
- Plant height.—About 5 meters.
- Plant diameter or spread.—About 5 meters.
- Lateral branch description.—Length: About 20 cm. Diameter: About 6 mm. Internode length: About 3.6 cm. Texture: Smooth, glabrous. Strength: Strong. Color, immature: 146C. Color, mature: 195A.
-
- Foliage description:
-
- Arrangement.—Alternate, simple.
- Length.—About 11 cm.
- Width.—About 10 cm.
- Shape.—Oblong to ovate.
- Apex.—Obtuse to acute.
- Base.—Cordate.
- Margin.—Serrate.
- Texture, upper and lower surfaces.—Slightly pubescent.
- Venation pattern.—Pinnate.
- Color.—Developing foliage, upper and lower surfaces: 144A. Fully expanded foliage, upper surface: Spring and summer, 136A; late summer and fall, 136A. Fully expanded foliage, lower surface: Spring and summer, 137C; late summer and fall, 137C. Venation, upper surface: Spring and summer, 139A; late summer and fall, 139A. Venation, lower surface: Spring and summer, 139C; late summer and fall, 139C.
- Petiole.—Length: About 11 mm. Diameter: About 1.9 mm. Texture, upper and lower surfaces: Pubescent. Color, upper surface: Spring and summer, 146D; late summer and fall, 146D. Color, lower surface: Spring and summer, 146D; late summer and fall, 146D.
-
- Flower description: Male inflorescences are catkins, color prior to elongation 194C. Female inflorescence style color 048C.
- Nuts description:
-
- Length.—About 19 mm. Width: About 18 mm. Depth: About 16.5 mm. Shape: Round. Nut shape index [(Width+Depth)/2*Length]=0.91. Nut compression index (Width/Depth)=1.09. Nut shell color: 164A. Nut weight: About 2.24 grams. Kernel weight: About 1.06 grams. Kernel percentage (kernel weight/nut weight): About 47%.
-
- Disease/pathogen/pest resistance: Plants of the new Corylus are susceptible to eastern filbert blight caused by the fungus Anisogramma anomala (Peck) E. Muller. Plants of the new Corylus are moderately resistant to bud mites (Phytoptus avellanae Nal.), while plants of ‘Tonda Gentile delle Langhe’ are highly susceptible, and plants of ‘Barcelona’ are highly resistant.
- Temperature tolerance: Plants of the new Corylus have been observed to tolerate temperatures from about −10° C. to about 38° C. in the field in Corvallis, Oreg.
Nut yields of ‘Tonda Pacifica’ are similar or slightly higher than nut yields for ‘Barcelona’. Since ‘Tonda Pacifica’ trees are smaller than ‘Barcelona’ trees, the nut yield efficiency for ‘Tonda Pacifica’ is significantly higher than that for ‘Barcelona’. The nuts of ‘Tonda Pacifica’ are borne in clusters of 3-4 in husks 75 to 100% longer than the nuts. The husks are slit down the side and the nuts fall free at maturity. The harvest date for the nuts of ‘Tonda Pacifica’ is estimated to be 7-10 days before ‘Barcelona’. ‘Tonda Pacifica’ produces fewer blank nuts and fewer defects than ‘Barcelona’. Raw kernels have a moderate amount of fiber attached to the pellicle, which can be mostly removed by light roasting and rubbing. Blanching scores for ‘Tonda Pacifica’ are better than for ‘Barcelona’ and ‘Tonda Gentile delle Langhe’.
Fingerprinting with microsatellite markers, also known as simple sequence repeat (SSR) markers, was performed. Twenty nine marker loci (Table 1) were selected from those published for hazelnut (Bassil et al. (2005) J. Amer. Soc. Hort. Sci., 130:543-549; Bassil et al. (2005) Acta Hort., 686:105-110; Boccacci et al. (2005) Molec. Ecol. Notes, 5:934-937; Gürcan et al. (2010) Tree Genetics and Genomes (available on-line as DOI 10.1007/s11295-010-0269-y); Gürcan et al. (2010) Molecular Breeding, 26:551-559). SSR markers have been used for fingerprinting hazelnut accessions (Boccacci et al. (2006) Genome 49:598-611; Gökirmak et al. (2009) Genetic Resources and Crop Evolution, 56:147-172; Gürcan et al. (2010) Plant Breeding, 129:422-434). Using primers designed for each SSR loci, hazelnut DNA was amplified by PCR. One of the two primers was fluorescently labeled with FAM, HEX, or NED, and the size of the amplified fragment was determined by capillary electrophoresis on an ABI 3100 instrument (Applied Biosystems; Foster City, Calif.). ‘Tonda Pacifica’ was found to exhibit different allele sizes at certain loci that allowed for it to be distinguish from other hazelnut genotypes such as its parent ‘Tonda Gentile delle Langhe’ and its grandparents ‘Barcelona’ and ‘Extra Ghiaghli’ (Table 2).
| TABLE 1 |
| Primers and annealing temperatures for the 29 microsatellite marker loci |
| used to fingerprint ‘Tonda Pacifica’ and five other hazelnut cultivars. |
| Locus | Tm | Allele Sizes | Repeat Motif |
| A613 | 60 | 149-177 | (TC)13(CA)12 |
| A614 | 60 | 125-156 | (TC)17(CA)10NNN(CA)6 |
| A616 | 60 | 136-162 | (AC)11 |
| A640 | 67 | 354-378 | (CT)15(CA)13 |
| B029b | 58 | 114-136 | (GA)13 |
| B619 | 60 | 146-180 | (TC)21 |
| B628 | 60 | 290-312 | (TC)7NN(CT)6 |
| B634 | 60 | 218-238 | (AG)15 |
| B657 | 60 | 210-228 | (AG)15 |
| B664 | 60 | 186-216 | (TC)21 |
| B665 | 60 | 177-203 | (CT)17 |
| B670 | 60 | 153-182 | (GA)19 |
| B671 | 60 | 221-249 | (AG)6NN(GA)17 |
| B706 | 60 | 168-206 | (CT)28 |
| B720 | 60 | 159-179 | (AG)14 |
| B732 | 60 | 140-156 | (GA)13 |
| B733 | 60 | 161-183 | (TC)15 |
| B741 | 60 | 176-194 | (GT)5(GA)12 |
| B749 | 60 | 200-210 | (TC)12 |
| B751 | 60 | 141-153 | (GA)15 |
| B758 | 60 | 154-176 | (CT)15 |
| B767 | 60 | 198-238 | (TC)15(AT)7 |
| B776 | 60 | 134-148 | (GA)17 |
| B795 | 60 | 296-332 | (TC)8Ns(CT)7Ns(CT)10Ns |
| (TC)5 | |||
| C115 | 60 | 167-225 | (TAA)5(GAA)12 |
| KG807 | 54 | 226-248 | (TAAA)AA(TAAA)2A |
| (TAAA)2 | |||
| KG810 | 56 | 366-392 | (AG)15 |
| KG827 | 67 | 264-282 | (CT)13AA(CA)7 |
| KG830 | 67 | 279-311 | (CT)14GTATT(CA)8 |
| Forward Primer (5′-3′) | Reverse Primer (5′-3′) | |
| Locus | (SEQ ID NO) | (SEQ ID NO) |
| A613 | Ned-CACACGCCTTGTCAC | R- |
| TCTTT (1) | CCCCTTTCACATGTTTGCTT | |
| (30) | ||
| A614 | Hex- | R- |
| TGGCAGAGCTTTGTCAG | GCAGTGGAGGATTGCTGA | |
| CTT (2) | CT (31) | |
| A616 | Fam- | R- |
| CACTCATACCGCAAAC | ATGGCTTTTGCTTCGTTTTG | |
| TCCA (3) | (32) | |
| A640 | F- | Fam- |
| TGCCTCTGCAGTTAGTCA | CGCCATATAATTGGGATG | |
| TCAAATGTAGG (4) | CTTGTTG (33) | |
| B029b | Ned- | R-AAGTTCACCCAAGAAATC |
| CAATTTACACCTCAGGG | CAC (34) | |
| AAGAG (5) | ||
| B619 | Fam- | R- |
| AGTCGGCTCCCCTTTTCTC | GCGATCTGACCTCATTTTTG | |
| (6) | (35) | |
| B628 | Fam-AATCCCCTCTAGCCC | R-CACAGAATATTTGTAATT |
| CATTA (7) | ACCACCACA (36) | |
| B634 | Hex-CCTGCATCCAGGACT | R- |
| CATTA (8) | GTGCAGAGGTTGCACTCA | |
| AA (37) | ||
| B657 | Ned- | R- |
| GAGAGTGCGTCTTCCT | AGCCTCACCTCCAACGAAC | |
| CTGG (9) | (38) | |
| B664 | Ned- | R- |
| CAAAGCCGTCGACAACAG | TTTGCATTTGATGCCGATAA | |
| (10) | (39) | |
| B665 | Hex- | R- |
| GCAACCACCAAATTGC | GCTTTTAAAGTCCACGCAT | |
| ACTA (11) | GA (40) | |
| B670 | Fam- | R- |
| CAACACTCACGTTTGG | TCTGTGTTGTTGGGAGTGGA | |
| TTGC (12) | (41) | |
| B671 | Hex- | R- |
| TTGCCAGTGCATACTCT | ACCAGCTCTGGGCTTAACAC | |
| GATG (13) | (42) | |
| B706 | Fam- | R-AGCAAAGAGGTAAGCAA |
| TGCATGAAATGGAATCA | ATTCA (43) | |
| CAGA (14) | ||
| B720 | Fam- | R- |
| CTCTGTGTCGGCTTTC | ATAAACCTCACGCCACACCT | |
| TGGT (15) | (44) | |
| B732 | Fam- | R- |
| GCCCTTCTTCTTTTCTG | AGTGCCACCTCAACAAAT | |
| CAA (16) | CC (45) | |
| B733 | Ned- | R- |
| CACCCTCTTCACCACC | CATCCCCTGTTGGAGTTTTC | |
| TCAT (17) | (46) | |
| B741 | Fam- | R- |
| GTTCACAGGCTGTTGG | CGTGTTGCTCATGTGTTGTG | |
| GTTT (18) | (47) | |
| B749 | Hex- | R- |
| GGCTGACAACACAGCA | TCGGCTAGGGTTAGGGTTTT | |
| GAAA (19) | (48) | |
| B751 | Fam- | R-AAACTCAAATAAAACCCC |
| AGCTGGTTCTTCGACA | TGCTC (49) | |
| TTCC (20) | ||
| B758 | Fam- | R- |
| TAATTTAAGCTGCCGT | TGCAAAATTGCATTGCTCAT | |
| GCAA (21) | (50) | |
| B767 | Fam- | R- |
| CCACCAACTGTTTCAC | GCGAAATGGAGCTCTTGA | |
| ACCA (22) | AC (51) | |
| B776 | Fam- | R- |
| TGTATGTACACACGGA | TGAGGGGAAGAGGTTTGA | |
| GAGAGAGA (23) | TG (52) | |
| B795 | Fam- | R- |
| GACCCACAAACAATAAC | TGGGCATCATCCAGGTCTA | |
| CTATCTC (24) | (53) | |
| C115 | Fam- | R-TCCAGATCTGCCTCCATA |
| CATTTTCCGCAGATAAT | TAAT (54) | |
| ACAGG (25) | ||
| KG807 | AAGCAAGAAAGGGATGG | Fam- |
| T(26) | CTTACAGATAAATGGCT | |
| CAAA (55) | ||
| KG810 | TCCTCACCAATCACACTA | Ned- |
| TTT (27) | TTATTCCACCAAAGTCTA | |
| CCTC (56) | ||
| KG827 | Fam- | R-GAGGGAGCAAGTCAAAG |
| AGAACTCCGACTAATAA | TTGAGAAGAAA (57) | |
| TCCTAACCCTTGC (28) | ||
| KG830 | Ned- | R-AAAGCAACTCATAGCTGA |
| TGGAGGAAGTTTTGAATG | AGTCCAATCA (58) | |
| GTAGTAGAGGA (29) | ||
| Tm = annealing temperature. | ||
| One of the two primers at each locus had a fluorescent label (Fam, Hex or Ned) to allow sizing with capillary electrophoresis. | ||
| TABLE 2 |
| Allele sizes in ‘Tonda Pacifica’ and five other hazelnut cultivars at 29 |
| microsatellite loci. The female parent of ‘Tonda Pacifica’ is ‘Tonda |
| Gentile delle Langhe’. The male parent is OSU 23.024, which is from a |
| cross of ‘Barcelona’ x ‘Extra Ghiaghli’. Note that one of the alleles of |
| ‘Tonda Pacifica’ is from ‘Tonda Gentile delle Langhe’, and the other is |
| from either ‘Barcelona’ or ‘Extra Ghiaghli’. |
| Cultivar |
| Tonda | ||||||
| Micro- | Gentile | |||||
| satellite | Tonda | delle | Extra | |||
| Locus | Pacifica | Langhe | Barcelona | Ghiaghli | Clark | Lewis |
| A613 | 157/167 | 151/157 | 151/159 | 167/169 | 151/177 | 151/177 |
| A614 | 134/148 | 125/134 | 125/131 | 125/148 | 131/131 | 131/148 |
| A616 | 148/158 | 148/150 | 142/150 | 150/158 | 148/150 | 148/150 |
| A640 | 368/374 | 354/368 | 354/374 | 374/374 | 354/354 | 354/354 |
| B029b | 118/120 | 120/124 | 124/130 | 118/132 | 124/130 | 124/124 |
| B619 | 164/170 | 148/164 | 156/170 | 164/174 | 156/168 | 156/168 |
| B628 | 297/297 | 297/299 | 293/297 | 293/297 | 297/297 | 293/297 |
| B628 | 298/298 | 298/300 | 294/298 | 294/298 | 298/298 | 294/298 |
| B634 | 226/226 | 226/226 | 226/226 | 226/226 | 230/234 | 226/234 |
| B657 | 210/226 | 218/226 | 218/222 | 210/222 | 210/218 | 214/222 |
| B664 | 186/206 | 186/206 | 206/214 | 206/208 | 192/204 | 188/208 |
| B665 | 178/185 | 185/189 | 189/195 | 178/189 | 195/195 | 193/195 |
| B670 | 169/182 | 159/182 | 153/159 | 169/177 | 173/177 | 159/173 |
| B671 | 227/237 | 237/241 | 223/227 | 227/247 | 223/247 | 223/223 |
| B706 | 174/206 | 190/206 | 168/190 | 172/174 | 190/200 | 190/200 |
| B720 | 161/167 | 167/167 | 161/167 | 165/179 | 159/159 | 159/179 |
| B732 | 140/154 | 140/154 | 150/154 | 140/154 | 150/154 | 150/150 |
| B733 | 171/173 | 171/173 | 171/173 | 171/171 | 171/179 | 173/179 |
| B741 | 176/186 | 176/184 | 176/186 | 176/184 | 176/186 | 176/186 |
| B749 | 206/208 | 206/208 | 208/208 | 208/208 | 208/208 | 206/208 |
| B751 | 143/153 | 149/153 | 143/153 | 143/147 | 147/151 | 151/153 |
| B758 | 160/160 | 160/168 | 168/168 | 160/160 | 168/168 | 168/168 |
| B767 | 198/216 | 211/216 | 211/237 | 198/204 | 204/235 | 211/235 |
| B776 | 136/136 | 136/136 | 134/136 | 134/136 | 136/136 | 136/136 |
| B795 | 312/330 | 312/330 | 330/330 | 296/310 | 330/330 | 330/330 |
| C115 | 173/182 | 173/173 | 193/193 | 182/193 | 193/193 | 193/193 |
| KG807 | 246/248 | 234/248 | 234/248 | 248/250 | 226/248 | 234/248 |
| KG810 | 378/382 | 374/378 | 374/378 | 378/382 | 378/380 | 378/382 |
| KG827 | 268/282 | 268/268 | 280/282 | 276/282 | 270/276 | 270/280 |
| KG830 | 291/295 | 291/295 | 291/295 | 289/295 | 295/303 | 291/303 |
Claims (1)
1. A new and distinct Corylus plant named ‘Tonda Pacifica’ as illustrated and described.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/928,688 USPP22715P2 (en) | 2010-12-16 | 2010-12-16 | Corylus plant named ‘Tonda Pacifica’ |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US12/928,688 USPP22715P2 (en) | 2010-12-16 | 2010-12-16 | Corylus plant named ‘Tonda Pacifica’ |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| USPP22715P2 true USPP22715P2 (en) | 2012-05-08 |
Family
ID=46002240
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| US12/928,688 Active USPP22715P2 (en) | 2010-12-16 | 2010-12-16 | Corylus plant named ‘Tonda Pacifica’ |
Country Status (1)
| Country | Link |
|---|---|
| US (1) | USPP22715P2 (en) |
-
2010
- 2010-12-16 US US12/928,688 patent/USPP22715P2/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| AS | Assignment |
Owner name: THE STATE OF OREGON, ACTING BY AND THROUGH THE STA Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNOR:MEHLENBACHER, SHAWN A.;REEL/FRAME:025661/0288 Effective date: 20110113 |
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