USPP10956P - Hop variety named `Columbus` - Google Patents

Hop variety named `Columbus` Download PDF

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USPP10956P
USPP10956P US08/409,908 US40990895V US10956P US PP10956 P USPP10956 P US PP10956P US 40990895 V US40990895 V US 40990895V US 10956 P US10956 P US 10956P
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methyl
columbus
humulene
epoxide
hop
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Gregory K. Lewis
Charles E. Zimmermann
Henry Hazenberg
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HUSA-CEZ LLC
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Hopunion USA
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/88Vitaceae, e.g. Vitus [grape]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/08Fruits

Abstract

A new and distinct variety of hop, Humulus lupulus L., named Columbus, has a superior yield of cones and a superior content of alpha acids in its resin. The new variety was cultivated as a result of a cross at a nursery near Prosser, Wash., United States, and has been asexually reproduced in and about Yakima, Wash., United States.

Description

CROSS-REFERENCE TO RELATED APPLICATION
This application is a continuation of U.S. patent application Ser. No. 08/159,859, filed Nov. 30, 1993, now abandoned.
TECHNICAL FIELD
The field of the present invention is hops.
BACKGROUND OF THE INVENTION
Of conventional hop varieties, there are at least twenty named commercially grown hop varieties in the United States today, and some seventy-five to eighty varieties that are grown around the world. The varieties differ as to assorted physical features such as vine color and structure, as well as cone number, location, size and shape.
Commercial varieties of hop are female hops that exist as a perennial rootstock that produces an annual flush of above-ground, or aerial, vegetation. The perennial rootstock is comprised of a woody crown with a number of main roots and sub-roots that are used for water uptake, nutrient uptake, and winter starch storage. Over the winter, the woody crown supports many buds that develop into main vines (shoots) in the spring, emerge from the ground, and extend to produce the annual above-ground vegetation. In nature, with the onset of frosts in the fall, the above-ground portion of the plant "dies-back" to ground level, and only the perennial rootstock survives into the following year.
A main vine consists of a single flexible cylinder that produces pairs of leaves at nodes separated by variable distances along the length of the vine. The length of main vine between a pair of nodes is known as an internode. A bud is produced in the junction between leaf and vine, which junction is also known by the botanical term axil. Such a botanical term, as well as other botanical terms herein, are generally known to those of skill in the art, and can also be found in standard botanical texts such as Simon, E.W., et al., Lawson's Textbook of Botany--Revised, University Tutorial Press Ltd., London, England, 14th Ed. 1966. The bud grows and develops into a lateral, also known as a side-shoot or side-arm, with the same basic vegetative structure as the main vine. The buds on the laterals develop into groups of flowers that later mature into cones (strobiles). Because the laterals produce and support the cones of the hop, the structure of the laterals strongly influences the number of cones produced and hence the ultimate commercial yield of a variety.
The cones are the useful brewing commodity, and are referred to as "hops" in the commercial industry. Each cone is a group of flowers (inflorescence) consisting of sepals and petals (bracts and bracteoles) mounted on a central strig (or rachis). The resultant structure resembles a small pine cone or fir cone but is non-woody and green in color. In nature, the bracteoles support the seed of the plant.
Complete descriptions of the botanical structure of the hop plant are given in Burgess, "Hops--Botany, Cultivation and Utilization," Weed Crop Series, Interscience Publishers, Inc., New York, 1964 and Neve, "Hops," von Nostrand Reinhold, New York, 1991.
A trellis of poles and wire, commonly 18 feet in height and known as a "high trellis," supports strings that the main vines climb. The main vines grow to the top of the trellis, where they produce the laterals that support the groups of flowers that develop into the cones. Typically, the laterals bear cones only in the upper parts of the plant (i.e., closer to the main vine); the distance from the main vine varies from one variety to another. Under an alternative growing system, the "HopUnion Low Trellis Hop Growing System," the vines are grown on a trellis of single rows of 10 feet in height, like wine grapes (the 10 foot trellis is referred to herein as a "low trellis"). (Lewis, "The HopUnion U.S.A., Inc.--Low Trellis Hop Production System," Proc. Tech. Comm. of the IHGC of the XXXVIIIth International Hop Congress, Hereford, U.K. Published Rijksstation voor Landbouwtechniek, Merelbeke/Gent, Belgium, 17-37, 1990.) Under either system, when the cones are physiologically mature (ripe), the cones are harvested, then dried and compressed into bales for market, where the cones are typically used for the productuion of beverages such as beers, ales and related alcohol-containing liquids.
Different varieties of hops have differences in the chemical constituents of the lupulin of their cones. Accordingly, chemical analysis of such differences can be useful in distinguishing one hop variety from another. Such analysis is generally performed by high pressure liquid chromatography ("HPLC") of the soft resins along with gas liquid chromatography ("GLC") of the essential oils. Recently, Kenny developed a key for differentiation of hop varieties based on chemical analysis of the soft resins. (Kenny, "Identification of U.S. Grown Hop Cultivars by Hop Acid and Essential Oil Analyses," J. Amer. Soc. Brewing Chemists 48(1):3-8, 1990.) Further, Peacock and McCarty, as well as Kenny, have developed keys for the differentiation of hop varieties based on chemical analysis of the essential oils. (Peacock and McCarty, "Varietal Identification of Hops and Hop Pellets," Master Brewer Assoc. of the Americas Technical Quarterly 27:81-85, 1992; Kenny, supra.) These keys are based on gross differences in the amounts of, and ratios between, major constituents, which differences are large enough to overcome variation inherent in the analytical analysis of biological material.
SUMMARY OF THE INVENTION
The present invention provides a new variety of hop (Humulus lupulus L.), which has been named `Columbus`. The new variety comprises a superior yield of cones and, as discussed below, a superior content of alpha acids in its soft resins. Columbus is particularly suited for the production of beverages such as beers, ales and related alcohol-containing liquids.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 depicts a section of Columbus vine near harvest.
FIG. 2 depicts a low trellis planting of Columbus.
FIG. 3 depicts early season growth of Columbus with an internode length of about 3 inches.
FIG. 4 depicts Columbus on a low trellis and shows the density of the cones in the canopy (cones per lateral node).
FIG. 5 depicts the physical appearance of Columbus cones.
DETAILED DESCRIPTION OF THE INVENTION
The present invention provides a new variety of hop (Humulus lupulus L.), named `Columbus,` that has a superior yield of cones and a superior content of alpha acids in its soft resins. Columbus may be classed as a bitter hop (i.e., high in alpha acids content). Columbus was bred as the result of an open-pollination cross that was carried out in 1982 at a small hop nursery near Prosser, Wash. U.S.A, and was known by the identification number 9/17. Pursuant to growth, analysis, and testing, Columbus was identified and selected from numerous candidates arising from the cross. The exact parentage of Columbus is unknown. The breeding nursery that produced Columbus contained 20-30 female plants from which seed was collected en masse, and an approximately identical number of male plants were growing and flowering at the time that pollination occurred. Columbus is a diploid hop.
Set forth below are various characteristics that distinguish Columbus from conventional hop varieties. Columbus is initially distinguishable on the basis of its physical characteristics, including the location of its cones, the internode distance, number of cones per node, the yield of its cones, and the physical characteristics of the cones. Columbus is also distinguishable on the basis of its chemical characteristics, which are determined by chemical analyses of the soft resins and essential oils. Additionally, methods for asexual reproduction of Columbus are discussed.
I. Physical Characteristics of Columbus A. Location of Cones
Columbus bears a high number of cones on its laterals down to a height of about 4 feet from the ground, when grown on a high trellis. A close-up Figure depicting in detail the vine structure of Columbus is presented as FIG. 1. Conversely, most conventional hop varieties on a high trellis do not bear significant numbers of cones below about 8 feet from the ground.
On a low trellis, Columbus produces significant numbers of cones down to ground level, and cones can even be found on untrained side-shoots running along the ground. (FIG. 2.) Conversely, most conventional hop varieties on a low trellis produce cones down to about 4 to 8 feet from the ground.
B. Internode Distance
The internode distance on a main vine of any hop variety is important to the yield of cones because the shorter the internode length, the greater the number of yielding laterals for a given length of main vine. Columbus has a shorter than average mature internode length, about 8" to 12", with an early season internode distance of about 3". (FIG. 3.) Conversely, in conventional hop varieties such as Nugget, the mature internode length varies from 10" to 16", with an early season internode distance of about 8". There are also "dwarf hops" in which the mature internode length varies between 5" to 8".
C. Growth Characteristics
Although the early season growth rate and length of time for hop vines to reach the top wire of a trellis vary widely due to factors such as climatic conditions and growth and harvest management, the early season growth of Columbus is generally faster than the most aroma hops (such as Tettnang, Perle, Centennial, Saaz, Spalt, Mt. Hood, Liberty, Hallertau, Hersbruck, and Fuggle), and also faster than the quicker-growing aroma hops such as Cascade and Willamette. Such aroma hops are generally first trained between May 7 and May 10, and reach the top wire between June 20 and June 30. The aroma hop Cluster and the bitter hops Galena and Chinook are typically trained about May 15 and reach the top wire from about June 20 to about June 30. The bitter hops Nugget, Olympic, Eroica, and Columbus are typically trained about May 25 and reach the top wire about June 27 to about July 7. Among this latter group, Columbus typically grows slower than the other hop varieties at first, but then accelerates its growth and can grow to the top faster than Nugget on occasion. In other words, Columbus is a late maturing hop, and has an early growth rate that is typical for bitter hops and for Cluster, but it is a slow starter.
D. Habit
In common with most other hop varieties, the main vines of Columbus are originally green in color but turn to dark red to purple as the season progresses. The main vines are ridged, and along the ridges are hooked hairs that adhere to the supporting trellis. A characteristic feature of Columbus vines, as discussed above, is the short internodes.
Although the lateral length of a hop variety can vary greatly due to factors such as climate and management practices, the laterals of Columbus are typically longer than those of aroma varieties, are typically similar in length to those of Galena and Nugget, and are typically shorter than Cluster, Chinook, Olympic and Eroica. A generalization of comparative lateral lengths is as follows:
              TABLE 1______________________________________Hop Variety          Length of Lateral______________________________________Mt. Hood, Liberty, Saaz, Spalt, Perle,                1'-2'Hallertau, Tettnang, Hersbruck, Fuggle,CentennialCascade, Willamette  1.5'-2.5'Columbus, Galena, Nugget                2.0'-3.0'Cluster, Chinook, Olympic, Eroica                2.5'-3.5'______________________________________
Although most commercial hops have longer laterals in the head and much shorter laterals in the lower reaches of the vine. Columbus tends to have approximately even length laterals along the length of the vine. The sub-branches of the laterals develop as bunches of cones, and as set forth below, Columbus has an unusually high number of cones in each lateral bunch, typically greater than any other commercial variety.
The head of the vine is also subject to variation depending on such factors as climatic conditions and management practices, but Columbus can be generally grouped with Cascade. Willamette, Nugget and Galena as having a moderate head, although the head of Columbus is slightly smaller and neater than typical. The head of Columbus also appears more dense than most other varieties, apparently because more laterals are developed from an equivalent vine length due to the short internode distance discussed above.
In terms of overall vine morphology, Columbus tends to be stronger than the aroma varieties but slighter than the other bitter hops and Cluster. As noted above, the overall vine morphology also gives the appearance of being more dense than other commercial hop varieties.
E. Leaf Morphology
The leaf morphology of Columbus is typical for most commercial hop varieties, and may be summarized as set forth in the table below.
              TABLE 2______________________________________    Early Main Vine                Late Main Vine                             LateralCharacter    Leaves      Leaves       Leaves______________________________________Number   2/node      2/node       2/nodeLobes    cordate - 3 35           cordate - 3Width    3-5"        4-7"         1-2"Length   3-5"        4-7"         1-2"Upper Surface    smooth      smooth       smoothTextureLower Surface    fairly rough                rough        slightly roughTextureColor    med-dark green                dark green   med-dark greenMargins  lightly serrated                moderately serrated                             lightly serrated______________________________________
The leaf color of Columbus is also similar to other commercial hop varieties, although in the early part of the growing season when Columbus is slower growing than other bitter hops and Cluster, Columbus has a darker leaf, while later in the season when Columbus is faster growing, the leaf of Columbus is similar to other hops in its class. The leaf of Columbus is typically not as dark as most of the slower growing aroma hops.
F. Number of Cones per Node
Typically, cones are found in high number clusters borne at nodes on a lateral that are close to the main vine ("basal lateral nodes"). Progressively fewer cones are found in the clusters located at nodes progressively farther away from the main vine. Ultimately, the lateral terminates in a tip bearing a single cone. Columbus yields a high number of cones at the basal lateral nodes as well as at nodes located farther away from the main vine. (FIG. 4.) The basal lateral nodes of Columbus have produced up to 42 cones. Conversely, conventional low yielding hop varieties typically bear clusters of about 8-16 cones at basal lateral nodes, while conventional high yielding varieties typically bear clusters of about 16-28 cones at basal lateral nodes.
G. Yield of Cones
Due to the above discussed characteristics, Columbus provides an outstanding hop variety comprising the following: a plant bearing cones much lower down the main vine; a reduced internode length on the main vine, which results in a greater number of yielding laterals per given length of main vine; and, greater numbers of cones at each node, which results in greater numbers of cones on each of the yielding laterals. Accordingly, Columbus produces an outstanding yield of cones, whether grown on a high trellis or a low trellis.
Yield data for many hop varieties are compared with yield data for Columbus in Table 3. All hops were grown on a high trellis.
              TABLE 3______________________________________        Washington State                     HopUnion          Five-Year Average                     1992 AverageVariety       Yield (lb/acre)                       Yield (lb/acre)______________________________________Low yielding varietiesPerle                1172              1366Tettnang           970               1140High yielding varietiesCluster              2018              2407Chinook              1938              2216Nugget                1950                                  2829Galena                1926                                  2502Columbus             --               3290______________________________________
(Hop Growers of America, Inc., 1991-1992 Annual Statistical Report, 37th Annual HGA Convention, Portland, Oreg., 1993.)
Below in Table 4, yield data from low trellis hops grown at HopUnion farms for 1990 and 1992 are compared with experimental yield data for Columbus from 1991 and 1992.
              TABLE 4______________________________________Yield Data for Low Trellis Hops        1990 Average                   1992 AverageVariety       Yield (lb/acre)                     Yield (lb/acre)______________________________________Perle        1557       1010Tettnang          1062              800Cascade              1415                                1570Chinook              1234                                1017Nugget                1712                                1175Galena                1800                                1500(Year: 1991)Columbus     2832       3408______________________________________
First year plantings of hops typically yield significantly less than mature plantings of the same varieties. However, a 1992 first year planting of Columbus on a low trellis yielded, over an area of about 4.5 acres, an average of 2547 lb/acre. Columbus produces such yields with a growth habit that presents no problems for ease of harvesting.
H. Physical Characteristics of the Cones
Cones are typically described by size (large, medium, or small) and by degree of compactness (loose, medium, or tight). Size and compactness are indicative of the degree of compression (internode length) between the nodes of the central strig that subtends (supports) the bracts and bracteoles. Also, cones are often described in terms of weight, most often expressed in milligrams (mg). Light cones are usually between 120 and 160 mg in weight, heavy cones usually between 160-220 mg (kiln dry basis). Often the description includes a brief comment on general shape of the cone, such as round, ovoid, plump, etc.
Columbus cones are normally medium in length, typically about 1.25", medium to tight in compactness, and typically about 1" in diameter. Further, Columbus cones are typically quite rounded and plump, and weigh about 180-250 mg (kiln dry basis) at maturity. (FIG. 5.) Conversely, Chinook cones typically have bracts with the tips turned backwards (reflexed). Fuggle cones are typically square in cross-section. Nugget cones are typically compact, tight, and firm. Columbus cones are typically more dense than other commercial hop varieties, and hence typically heavier on a per cone basis. Columbus cones have little or no propensity to shatter when fresh or when properly dried.
I. Disease and Pest Resistance
The susceptibility of Columbus to downy mildew (Pseudoperunospora humuli) is similar to Galena and Cluster, which are less resistant than most other commercial hop varieties. Columbus is apparently resistant to Verticillium wilt (Verticillium dahliae). Tests have indicated that Columbus can become infected with Prunus necrotic ringspot virus, hop latent virus and American hop latent virus, although it is also possible to free Columbus of these viruses by standard meristem culture techniques. Columbus has not shown any symptoms of infection when carrying these viruses, as is usual for most commercial hop varieties. Columbus shows no unusual tolerance or sensitivity to damson hop aphid (Phorodon humuli) and two spotted mite (Tetranychus urticae).
J. Quality Profile
Based upon sensory analyses of dried/baled leaf hop samples, Columbus has a quality profile that is similar to most other commercial hop varieties. Columbus has a mild, gentle aroma with no particular, peculiar character. However, Columbus is distinguishable from the commercial aroma varieties and Cluster by virtue of its higher resin content, which is indicated by visual inspection of the open cone and the much greater number of lupulin glands present in Columbus.
II. Chemical Characteristics of Columbus A. Chemical Analyses in General
Chemical analysis of hops typically consists of analysis of the lupulin of the cones. The lupulin consists of the following chemical components and sub-components:
______________________________________Soft resins       alpha acids (humulones)                                 beta acids (lupulones)Hard resinsUncharacterized resinsWaxesTanninsEssential oils        hydrocarbons                               oxygenated compounds                               sulfur compounds______________________________________
Of the above components, the soft resins and essential oils are of particular relevance to the differentiation of hop varieties. In regard to the soft resins, there are three analogues of the alpha acids, i.e., humulone, ad-humulone, and cohumulone, and there are also three analogues of the beta acids, i.e., lupulone, ad-lupulone, and colupulone.
Due to their chemical characteristics, some hop varieties are referred to as aromatic, or low alpha, hops and are known to contribute good hop flavor and aroma. Other hop varieties are high in bittering potential (i.e., high in alpha acids) and are referred to as bitter or high alpha hops. Columbus may be referred to as such a bitter or high alpha hop. Hop varieties in an intermediate varietal classification have medium levels of alpha acids and are referred to as dual-purpose hops.
B. Soft Resin Characteristics
Table 5 lists the total amount of alpha and beta acids, the ratio of alpha acids to beta acids, and estimates of the relative proportions of cohumulone and colupulone for several high alpha hops. This data was determined by HPLC analysis according to standard methods well known to those having skill in the art. (See, e.g., American Society of Brewing Chemists, "Hops Method 12: Analysis of Hops, Hop Powders and Hop Pellets by High Pressure Liquid Chromatography," in "Methods of Analysis," The Society, St. Paul, Mn., 8th ed. 1991.) Table 5 also gives data on storage losses, which indicate the loss of alpha acids by oxidation. The data are expressed as ranges to account for inherent biological variation from sample to sample.
              TABLE 5______________________________________Parameter     Chinook   Nugget   Galena______________________________________Alpha acids (% w/w)         12.0-14.0 12.0-14.0                            11.0-13.0cohumulone (%)                  30-35                       24-30                                   36-42Beta acids (% w/w)              3.4-4.0                        4.0-5.5                             7.0-9.0colupulone (%)                   55-66                           50-55                                  60-65Alpha/beta ratio                  3.5-4.0                        2.5-3.0                               1.5-2.0Oil content (mls/100 g)            1.5-2.3                        1.2-2.0                               0.7-1.2Loss of alpha acids (%)              35-45                          20-35                                    20-306 months storageroom temperature______________________________________Parameter     Olympic   Eroica   Columbus______________________________________Alpha acids (% w/w)         11.5-13.5 11.0-13.0                            14.0-16.0cohumulone (%)                       38-43                                   30-35Beta acids (% w/w)              4.0-6.0                        4.0-5.5                             4.5-5.5colupulone (%)                   57-62                           67-70                                  55-60Alpha/beta ratio                  2.0-2.7                        2.2-2.8                               2.7-3.3Oil content (mls/100 g)            1.4-2.0                        0.5-1.3                               1.5-2.2Loss of alpha acids (%)              40-55                          35-50                                    60-806 months storageroom temperature______________________________________
Table 5 indicates the following: (a) Columbus has a higher content of alpha acids than the other high alpha hops; (b) Columbus' cohumulone and colupulone contents are higher than Nugget, lower than Eroica and Galena, and similar to Chinook; (c) Columbus' beta acid content is higher than Chinook and lower than Galena; (d) Columbus' alpha/beta ratio is higher than Galena or Olympic but lower than Chinook; and (e) Columbus more rapidly loses its alpha acids in ambient storage than the other varieties.
Table 5 shows that Columbus has an alpha acids content well in excess of 10% w/w. This distinguishes Columbus from the aromatic hops. Examples of such aromatic hops are listed in Table 6.
              TABLE 6______________________________________        Alpha acids contentVariety        (% w/w of cone)______________________________________Cascade      4.5-7.0Cluster                    5.5-8.5Fuggle                       4.0-5.5Hallertau                 3.5-5.5Hersbruck                 3.5-5.5Liberty                      3.0-5.0Mount Hood               5.0-7.5Perle                         7.0-9.5Saaz                            3.0-4.5Spalt                           3.0-5.5Tettnang                     4.0-5.0Willamette                  4.0-6.0______________________________________
As discussed above, Kenny developed a key for identification of hop varieties based on the soft resin components. The application of the key of Kenny to Columbus is depicted in Table 7. At each decision point, certain varieties are eliminated according to a yes/no decision. The path to Columbus is shown by the double arrow. In the key depicted in Table 5, as well as in the other keys discussed herein, steps from the published key that did not eliminate any varieties in the present analysis have been deleted.
              TABLE 7______________________________________           Major U.S. Variety Eliminations______________________________________1.  Fresh alpha + beta > 15%               →                     NO   Cascade, Cluster,    and Fresh Alpha > 10%      Fuggle, Hallertau,                          Hersbruck, Liberty,.arrow-down dbl.  YES               Mt. Hood, Perle, Saaz,      (High alpha hops)                    Spalt, Tettnang,                                  Willamette2.  Cohumulone > 40%               →                     YES  Eroica.arrow-down dbl.  NO3.  Colupulone > 60%               →                     YES  Galena.arrow-down dbl.  NO4.  Cohumulone < 30%               →                     YES  Nugget.arrow-down dbl.  NO5.  Alpha/beta ratio > 3.0               →                     YES  Chinook (3.5-4.0).arrow-down dbl.    →                     NO   Olympic (2.0-2.7)Columbus(2.7-3.3)______________________________________
Table 7 relates to hops with a hop storage index, or HSI, well below 0.300, which indicates that the alpha and beta acids of the hops are fresh when analyzed. In Table 7, step 1 eliminates all the aromatic hops on the basis of their low content of alpha acids and low alpha plus beta acids. Step 2 eliminates Eroica on the basis of high cohumulone content. Step 3 eliminates Galena on the basis of high colupulone content. (Eroica is again eliminated at this step.) Step 4 eliminates Nugget on the basis of low cohumulone content. Step 5 eliminates Chinook on the basis of a high alpha/beta ratio, while Olympic is eliminated on the basis of a low alpha/beta ratio. Note, Chinook is also eliminated on the basis of its higher beta acid content.
Accordingly, Columbus is differentiated from all major U.S. varieties based on its soft resins content. Similarly, Columbus is distinguished from the minor U.S. hop varieties, except Aquila, Banner, and Centennial, based on soft resins content. However, Columbus is distinguished from Aquila, Banner, and Centennial by its essential oils content, as discussed below.
C. Essential Oils Characteristics
Detailed analysis of the essential oils allows a further differentiation of Columbus from other hops. Table 8 further distinguishes some of the varieties discussed above in Table 7, while Table 9 distinguishes varieties not distinguished above.
Extraction and analysis of essential oils are performed according to methods well known to those having skill in the art. Preferably, the essential oils are extracted by steam distilling previously dried hops for about four hours (with the steam of the distillation condensed using a cold-finger condenser). The resultant essential oils are then examined by GLC analysis.
GLC analysis for various hops are presented as Appendices 1 and 2 hereto as follows:
Appendix 1: Detailed essential oil analysis results of many of the world's hop varieties including all U.S. grown varieties except Mount Hood. Columbus is the final entry.
Appendix 2: Detailed GLC traces and listings of constituent peaks from essential oils analysis. Columbus is compared with the other U.S. high alpha hops, Galena, Chinook, and Nugget.
As with the soft resins, keys can be used to differentiate by essential oils content. Only United States varietal differentiations are depicted below in Tables 8 and 9, but non-United States varietal eliminations are listed in Peacock and McCarty, supra, and can be readily utilized by those having skill in the art to distinguish between those varieties and Columbus.
              TABLE 8______________________________________Key of Kenny          Major U.S. variety eliminations______________________________________1.  β-Farnesene content > 1%                →                      YES  Cascade, Fuggle,.arrow-down dbl.    NO               Saaz, Spalt                     Tettnang, Willamette2.  Humulene/        →                      NO   Eroica, Olympic    β-Caryophyllene ratio > 1.5.arrow-down dbl.    YES3.  Presence of 2-Tridecanone/                →                      NO   Galena    presence of a peak @        (*see note in text)    34.38 > 1.0%.arrow-down dbl.    NO4.  β-Caryophyllene/                →                      YES  Cluster, Nugget    ∝-copaene > 20.arrow-down dbl.    NOChinook  Columbus(12.1)     (13.4)______________________________________
Table 8 was prepared using the data provided in Appendix 1. As shown in Table 8, step 1 eliminates the high β-farnesene hops, i.e., Cascade, Fuggle, Saaz, Spalt, Tettnang, and Willamette. Step 2 eliminates the low humulene varieties, i.e., Eroica and Olympic. Step 3 eliminates Galena because Columbus is unique for having no 2-tridecanone, and Columbus lacks a large peak found in Galena at 34.38 minutes (the peak is greater than 1% of the measured components of Galena). Step 4 eliminates both Cluster and Nugget. The remaining varieties are Columbus and Chinook. As shown in Table 7, Columbus is differentiated from Chinook by Columbus' lower alpha/beta ratio. In regard to some other varieties, Perle is eliminated by virtue of its low alpha acids content, while Columbus is distinguishable from Hersbruck, Hallertau, Liberty, and Mount Hood because Columbus has a much higher alpha acids content.
              TABLE 9______________________________________Key of Peacock and McCarty (1992)        Major U.S. variety eliminations______________________________________Columbus.arrow-down dbl.1.  Humulene present              →                    YES  Aquila, Eroica.arrow-down dbl.    YES2.  β-Farnesene >              →                    YES  Cascade, Fuggle    5% Humulene               Saaz, Spalt,                         Tettnang, Willamette.arrow-down dbl.    NO3.  Humulene/Selinene > 20              →                    YES  Banner, Hallertau,                         Liberty, Mt. Hood,                         Perle.arrow-down dbl.    NO4.  Contains Eudesmol              →                    YES  Chinook, Hersbruck.arrow-down dbl.    NO5.  Cohumulone < 38%              →                    NO   Cluster, Galena.arrow-down dbl.    YESColumbus, Nugget,Olympic______________________________________
Table 9 depicts the key of Peacock and McCarty, which differentiates Aquila, Banner, and Centennial. The data from Appendix 1 is used for all steps except step 4, for which the data in Appendix 2 (which quantifies eusdesmol) is necessary. In Table 9, Step 1 eliminates Aquila and Eroica. Step 2 eliminates the six high β-farnesene hops. Step 3 eliminates Perle, Banner, Hallertau, Liberty, and Mount Hood. Step 4 eliminates Hersbruck and Chinook. Step 5 eliminates Cluster and Galena. Table 7 does not distinguish Columbus from Nugget and Olympic. However, as discussed above, Nugget and Olympic are distinguished from Columbus by each of Table 7 and Table 8.
Finally, the variety Centennial has not yet been distinguished. The data in Appendix 1 show that Centennial has a β-caryophyllene level of 2.649, while Columbus has a β-caryophyllene level of 10.236. Therefore, the varieties are distinguished because they are at virtually opposite ends of the scale for β-caryophyllene.
III. Asexual Reproduction (Propagation) of the Plant
Columbus is asexually reproduced according to methods well known to those having skill in the art. Such methods generally comprise one of three procedures: (a) taking rootstock cuttings known as "roots" or "strap cuts;" (b) converting vine material into woody root-like material in the field and dividing this material into "layered cuttings;" or (c) the use of softwood cuttings, which are also known as "mist cuttings," of green vegetative or shoot material, usually under greenhouse conditions. Further, traditional methods can be applied to make a cell tissue culture from Columbus. Such a cell tissue culture can be stored for extended periods, and gives rise to clones (i.e., asexual progeny) of Columbus. Columbus has been asexually reproduced on ranches associated with HopUnion USA, Inc., located in and about Yakima, Wash. U.S.A.
A. Asexual Reproduction by Taking Rootstock Cuttings
In the field, when main vines die back in the fall, their bases turn woody and swell with the deposition of starch reserves. These bases produce buds at the sites where the main vine nodes were previously located. The bases, usually 4"-8" long and between one-half and two inches thick, are removed by cutting with a sharp knife; other buds on the rootstock produce re-growth in the spring. The detached vine bases are then planted singly or in multiples, in pots or in the field.
Such a procedure typically provides up to a 15-fold increase over the starting number of plants. The increase may be lower where multiple rootstocks (up to 6-8) are to be planted at a single site, to form a single new composite plant.
B. Asexual Reproduction by Layered Cuttings
In the field, main vines are grown up a string, typically to a pre-determined height of about 10 feet. The string is then lowered and the newly grown main vines laid along the ground (for example, for about 8 feet). The string is then re-hung so that a portion of the main vines can renew vertical growth, but in a new location. Some main vines are left laying along the ground and are buried with soil, where the absence of light on the buried, green main vine stimulates it to turn woody, form buds at the nodes and swell with starch deposits. When the unburied portion of the vine has died back, the buried, now woody, portion is dug up and cut up into numerous layered cuttings, which are then replanted to give new plants. Such a procedure provides up to about a 50-fold increase over the starting number of plants.
In an alternative embodiment, new plants are placed at the bottom of deep, open trenches, preferably in a nursery. As the plants grow, soil is periodically pushed into the trench around the vines, thereby yielding layered cuttings in a vertical plane.
C. Asexual Reproduction by Softwood (Mist) Cuttings
Actively growing main vines of a plant are cut into sections comprising a half-node, a single node, or two nodes. Main vines for this purpose are preferably grown in a greenhouse, where growing conditions can be maximized. The cuttings are planted and stuck in a suitable medium, preferably with bottom heat and an intermittent water mist, further preferably under glass. The cuttings produce a rooted plant with new main vine growth about two to three weeks after removal from the original plant. These rooted softwood cuttings can be further grown in pots, in a nursery, or even in the final new plantation. Using such a procedure, it is possible to achieve up to about a 100-fold increase over the starting number of plants.
              APPENDIX 1______________________________________Detailed GLC analysis of assorted hop varieties.Analytical Conditions: see Kenny (1990), supra.OIL AREA PERCENTS OF COMMERCIAL HOPS______________________________________GC/          TENTATIVE IDENTI-FID  CMPD.   FICATION BY GC./ 21223   21053RT.  #       MASS SPECTROSCOPY                         AQUILA  AURORA______________________________________6.42 9       A-PINENE         0.105   0.1127.46 14      ISOBUTYL ISOBUTYRATE                         0.000   0.3268.10 16      8-PINENE         0.898   0.8179.11 18      MYRCENE          62.462  58.7319.77 22      2-METHYL-BUTYL   0.171   0.159        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.000   0.0769.96 24      2-METHYL-BUTYL   0.370   0.754        ISOBUTARATE10.2826      L-LIMONENE       0.243   0.22810.6227      B-PHELLANDRENE?  0.262   0.234        C10H1511.5932      trans-OCIMENE    1.542   0.20712.4535      2-METHYL-BUTYL   0.000   0.000        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.445   0.65612.9140      ISOAMYL ISOVALERATE                         0.000   0.00014.3143      METHYL 4-METHYL- 0.069   0.160        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.230   0.264        HEPTANOATE15.9851      METHYL OCTANOATE 0.317   0.50616.2655      NONANAL          0.048   0.08319.4173      METHYL NONANOATE 0.172   0.34319.5675      2-DECANONE,      0.139   0.379        A-YLANGENE19.9377      A-COPAENE        0.121   0.16121.0083      LINALOOL         0.368   0.99721.3188      A-GURJUNENE      0.000   0.00021.4289      7-METHYL-2-DECANONE                         0.062   0.21822.5693      C15H24 CALARENE? 0.000   0.00022.7295      A-BERGAMOTENE,   0.239   0.408        METHYL DECANOATE23.0196      2-UNDECANONE     0.448   1.40223.1097      2-DECANOL        0.000   0.16023.4499      8-CARYOPHYLLENE  4.104   4.40323.84102     METHYL DEC-4-ENOATE                         1.608   1.28624.03103     MW168 2-OL?      0.000   0.181        ME 43,81,110,10825.18114     B-FARNESENE      0.000   4.37126.10122     HUMULENE         1.193   14.14126.16123     METHYL DEC-4,8-  1.462   0.310        DIENOATE26.46129     WILEY# 123998 MW 204                         0.341   0.635        C15H2426.59131     2-DODECANONE     0.311   0.00027.32138     A-FARNESENE OR   0.270   0.132        ZINGIBERENE27.53139     CAS# 030021-74-0 0.000   0.00027.75143     A-MUUROLENE      0.114   0.18227.90144     B-SELINENE       2.421   0.10928.04145     A-SELINENE       2.745   0.13028.34147     METHYL           0.266   0.308        ?-UNDECENOATE29.16154     D-CADINENE       0.531   0.69629.39155     G-CADINENE       0.191   0.38130.34164     CAS# 016728-99-7 OR                         3.659   0.184        G-SELINENE30.73166     A-MUUROLINE      0.000   0.00031.15170     2-TRIDECANONE    1.620   1.08031.36171     GERANYL PROPANOATE                         0.275   0.00031.46172     MW 212 METHYL    0.000   0.000        ?-DODECENOATE32.28177     trans-GERANIOL   0.264   0.00032.54179     CAS# 000483-77-2 1.173   0.000        (CALAMENENE ?)34.38187     METHYL 3,6-      0.109   0.000        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.122   0.28239.11211     CARYOPHYLLENE OXIDE                         0.121   0.08340.23221     HUMULENE         0.000   0.530        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.177   0.00041.07225     HUMULENE         0.000   0.199        MONO-EPOXIDE II41.55228     HUMULENE         0.000   0.000        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         0.598   0.95043.88242     MW 220 ME 43,79,91                         0.170   0.26244.73246     T-CADINOL        0.214   0.00046.08255     T-MUUROLOL       0.000   0.000        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000   0.000        DIEPOXIDE?48.69268     FARNESOL         0.407   0.000______________________________________GC/          TENTATIVE IDENTI-FID  CMPD.   FICATION BY GC./ 21080   21287RT.  #       MASS SPECTROSCOPY                         BACKA   BANNER______________________________________6.42 9       A-PINENE         0.000   0.1107.46 14      ISOBUTYL ISOBUTYRATE                         0.000   0.1298.10 16      8-PINENE         0.275   0.8429.11 18      MYRCENE          15.376  65.3059.77 22      2-METHYL-BUTYL   0.075   0.391        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.000   0.1739.96 24      2-METHYL-BUTYL   0.000   0.988        ISOBUTARATE10.2826      L-LIMONENE       0.108   0.24310.6227      B-PHELLANDRENE?  0.000   0.273        C10H1511.5932      trans-OCIMENE    0.000   0.68112.4535      2-METHYL-BUTYL   0.000   0.068        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.281   0.38112.9140      ISOAMYL ISOVALERATE                         0.000   0.00014.3143      METHYL 4-METHYL- 0.298   0.093        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.000   0.413        HEPTANOATE15.9851      METHYL OCTANOATE 0.090   0.26916.2655      NONANAL          0.939   0.03719.4173      METHYL NONANOATE 0.075   0.18819.5675      2-DECANONE,      0.497   0.051        A-YLANGENE19.9377      A-COPAENE        0.420   0.17321.0083      LINALOOL         0.461   0.58321.3188      A-GURJUNENE      0.164   0.16621.4289      7-METHYL-2-DECANONE                         0.384   0.00022.5693      C15H24 CALARENE? 0.382   0.06922.7295      A-BERGAMOTENE,   0.000   0.243        METHYL DECANOATE23.0196      2-UNDECANONE     1.442   0.04623.1097      2-DECANOL        0.685   0.17323.4499      8-CARYOPHYLLENE  10.792  5.47823.84102     METHYL DEC-4-ENOATE                         0.588   0.91024.03103     MW168 2-OL?      0.394   0.000        ME 43,81,110,10825.18114     B-FARNESENE      0.439   0.00026.10122     HUMULENE         37.338  13.25626.16123     METHYL DEC-4,8-  0.000   0.157        DIENOATE26.46129     WILEY# 123998 MW 204                         1.342   0.475        C15H2426.59131     2-DODECANONE     0.000   0.00027.32138     A-FARNESENE OR   0.114   0.134        ZINGIBERENE27.53139     CAS# 030021-74-0 0.182   0.09427.75143     A-MUUROLENE      0.314   0.17327.90144     B-SELINENE       0.391   0.12628.04145     A-SELINENE       0.429   0.14028.34147     METHYL           0.000   0.000        ?-UNDECENOATE29.16154     D-CADINENE       1.688   0.87329.39155     G-CADINENE       1.075   0.47830.34164     CAS# 016728-99-7 OR                         0.263   0.126        G-SELINENE30.73166     A-MUUROLINE      0.201   0.10031.15170     2-TRIDECANONE    0.871   1.94531.36171     GERANYL PROPANOATE                         0.000   0.26931.46172     MW 212 METHYL    0.000   0.081        ?-DODECENOATE32.28177     trans-GERANIOL   0.000   0.00032.54179     CAS# 000483-77-2 0.359   0.082        (CALAMENENE ?)34.38187     METHYL 3,6-      0.000   0.484        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.236   0.13739.11211     CARYOPHYLLENE OXIDE                         2.753   0.09740.23221     HUMULENE         0.173   0.048        MONO-EPOXIDE I40.28223     MW222 ME 43,82   1.201   0.11341.07225     HUMULENE         9.016   0.176        MONO-EPOXIDE II41.55228     HUMULENE         0.124   0.000        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         1.910   0.21343.88242     MW 220 ME 43,79,91                         0.500   0.11344.73246     T-CADINOL        0.403   0.07046.08255     T-MUUROLOL       0.209   0.040        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.333   0.000        DIEPOXIDE?48.69268     FARNESOL         0.000   0.084______________________________________GC/          TENTATIVE IDENTI-                         21116FID  CMPD.   FICATION BY GC./ BREWERS 64100RT.  #       MASS SPECTROSCOPY                         GOLD    BULLION______________________________________6.42 9       A-PINENE         0.117   0.1107.46 14      ISOBUTYL ISOBUTYRATE                         0.169   0.2518.10 16      8-PINENE         0.988   0.9349.11 18      MYRCENE          69.050  67.1139.77 22      2-METHYL-BUTYL   0.344   0.469        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.068   0.1539.96 24      2-METHYL-BUTYL   1.267   1.053        ISOBUTARATE10.2826      L-LIMONENE       0.260   0.25410.6227      B-PHELLANDRENE?  0.328   0.324        C10H1511.5932      trans-OCIMENE    0.549   0.44112.4535      2-METHYL-BUTYL   0.096   0.045        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.298   0.54312.9140      ISOAMYL ISOVALERATE                         0.000   0.03814.3143      METHYL 4-METHYL- 0.000   0.089        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.105   0.205        HEPTANOATE15.9851      METHYL OCTANOATE 0.206   0.17416.2655      NONANAL          0.049   0.04019.4173      METHYL NONANOATE 0.105   0.20819.5675      2-DECANONE,      0.040   0.070        A-YLANGENE19.9377      A-COPAENE        0.166   0.14921.0083      LINALOOL         0.461   0.44121.3188      A-GURJUNENE      0.039   0.07021.4289      7-METHYL-2-DECANONE                         0.000   0.00022.5693      C15H24 CALARENE? 0.085   0.06422.7295      A-BERGAMOTENE,   0.105   0.154        METHYL DECANOATE23.0196      2-UNDECANONE     0.000   0.11923.1097      2-DECANOL        0.179   0.15623.4499      8-CARYOPHYLLENE  6.305   6.05423.84102     METHYL DEC-4-ENOATE                         0.566   0.83724.03103     MW168 2-OL?      0.000   0.044        ME 43,81,110,10825.18114     B-FARNESENE      0.000   0.00026.10122     HUMULENE         10.326  10.52526.16123     METHYL DEC-4,8-  1.153   0.252        DIENOATE26.46129     WILEY# 123998 MW 204                         0.433   0.396        C15H2426.59131     2-DODECANONE     0.000   0.04727.32138     A-FARNESENE OR   0.177   0.169        ZINGIBERENE27.53139     CAS# 030021-74-0 0.104   0.08427.75143     A-MUUROLENE      0.172   0.13527.90144     B-SELINENE       0.378   0.43328.04145     A-SELINENE       0.410   0.49528.34147     METHYL           0.000   0.000        ?-UNDECENOATE29.16154     D-CADINENE       0.864   0.79029.39155     G-CADINENE       0.443   0.38730.34164     CAS# 016728-99-7 OR                         0.080   0.084        G-SELINENE30.73166     A-MUUROLINE      0.091   0.08331.15170     2-TRIDECANONE    0.348   0.80231.36171     GERANYL PROPANOATE                         0.257   0.49331.46172     MW 212 METHYL    0.000   0.000        ?-DODECENOATE32.28177     trans-GERANIOL   1.003   0.06032.54179     CAS# 000483-77-2 0.000   0.051        (CALAMENENE ?)34.38187     METHYL 3,6-      0.000   0.126        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.000   0.26739.11211     CARYOPHYLLENE OXIDE                         0.082   0.08440.23221     HUMULENE         0.097   0.227        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.047   0.10741.07225     HUMULENE         0.115   0.131        MONO-EPOXIDE II41.55228     HUMULENE         0.057   0.000        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         0.073   0.46943.88242     MW 220 ME 43,79,91                         0.000   0.26544.73246     T-CADINOL        0.078   0.07346.08255     T-MUUROLOL       0.046   0.044        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000   0.000        DIEPOXIDE?48.69268     FARNESOL         0.586   0.472______________________________________GC/          TENTATIVE IDENTI-        21507FID  CMPD.   FICATION BY GC./ 56013   CENTE-RT.  #       MASS SPECTROSCOPY                         CASCADE NNIAL______________________________________6.42 9       A-PINENE         0.098   0.1557.46 14      ISOBUTYL ISOBUTYRATE                         0.110   0.1278.10 16      8-PINENE         0.933   1.1259.11 18      MYRCENE          68.051  77.9119.77 22      2-METHYL-BUTYL   0.260   0.372        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.096   0.2349.96 24      2-METHYL-BUTYL   0.527   0.911        ISOBUTARATE10.2826      L-LIMONENE       0.233   0.32410.6227      B-PHELLANDRENE?  0.337   0.358        C10H1511.5932      trans-OCIMENE    0.132   0.08012.4535      2-METHYL-BUTYL   0.000   0.094        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.210   0.33312.9140      ISOAMYL ISOVALERATE                         0.043   0.12614.3143      METHYL 4-METHYL- 0.055   0.114        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.091   0.168        HEPTANOATE15.9851      METHYL OCTANOATE 0.093   0.19316.2655      NONANAL          0.051   0.00019.4173      METHYL NONANOATE 0.072   0.10119.5675      2-DECANONE,      0.044   0.000        A-YLANGENE19.9377      A-COPAENE        0.118   0.08821.0083      LINALOOL         0.312   0.47021.3188      A-GURJUNENE      0.000   0.05421.4289      7-METHYL-2-DECANONE                         0.000   0.00022.5693      C15H24 CALARENE? 0.070   0.00022.7295      A-BERGAMOTENE,   0.275   0.050        METHYL DECANOATE23.0196      2-UNDECANONE     0.110   0.00023.1097      2-DECANOL        0.175   0.00923.4499      8-CARYOPHYLLENE  4.059   4.64923.84102     METHYL DEC-4-ENOATE                         0.333   0.80924.03103     MW168 2-OL?      0.000   0.000        ME 43,81,110,10825.18114     B-FARNESENE      5.285   0.00026.10122     HUMULENE         10.063  5.69926.16123     METHYL DEC-4,8-  0.219   2.229        DIENOATE26.46129     WILEY# 123998 MW 204                         0.325   0.239        C15H2426.59131     2-DODECANONE     0.000   0.00027.32138     A-FARNESENE OR   0.311   0.110        ZINGIBERENE27.53139     CAS# 030021-74-0 0.087   0.00027.75143     A-MUUROLENE      0.107   0.17027.90144     B-SELINENE       0.508   0.00028.04145     A-SELINENE       0.533   0.05028.34147     METHYL           0.195   0.000        ?-UNDECENOATE29.16154     D-CADINENE       0.624   0.42029.39155     G-CADINENE       0.306   0.24630.34164     CAS# 016728-99-7 OR                         0.071   0.000        G-SELINENE30.73166     A-MUUROLINE      0.066   0.00031.15170     2-TRIDECANONE    0.836   0.56531.36171     GERANYL PROPANOATE                         0.402   0.21831.46172     MW 212 METHYL    0.109   0.069        ?-DODECENOATE32.28177     trans-GERANIOL   0.092   0.76232.54179     CAS# 000483-77-2 0.000   0.000        (CALAMENENE ?)34.38187     METHYL 3,6-      0.047   0.098        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.148   0.00039.11211     CARYOPHYLLENE OXIDE                         0.075   0.07240.23221     HUMULENE         0.000   0.000        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.064   0.00041.07225     HUMULENE         0.157   0.114        MONO-EPOXIDE II41.55228     HUMULENE         0.000   0.000        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         0.490   0.16443.88242     MW 220 ME 43,79,91                         0.382   0.00044.73246     T-CADINOL        0.069   0.00046.08255     T-MUUROLOL       0.000   0.000        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000   0.000        DIEPOXIDE?48.69268     FARNESOL         0.093   0.000______________________________________GC/          TENTATIVE IDENTI-                         21043FID  CMPD.   FICATION BY GC./ CHALL- 21226RT.  #       MASS SPECTROSCOPY                         ANGER  CHINOCK______________________________________6.42 9       A-PINENE         0.078  0.1027.46 14      ISOBUTYL ISOBUTYRATE                         0.192  0.2708.10 16      8-PINENE         0.765  0.7689.11 18      MYRCENE          55.224 48.1059.77 22      2-METHYL-BUTYL   0.254  0.477        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.082  0.4479.96 24      2-METHYL-BUTYL   1.164  2.385        ISOBUTARATE10.2826      L-LIMONENE       0.204  0.21010.6227      B-PHELLANDRENE?  0.260  0.228        C10H1511.5932      trans-OCIMENE    0.259  0.06712.4535      2-METHYL-BUTYL   0.067  0.185        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.631  0.20012.9140      ISOAMYL ISOVALERATE                         0.000  0.39814.3143      METHYL 4-METHYL- 0.337  0.099        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.241  1.211        HEPTANOATE15.9851      METHYL OCTANOATE 0.323  0.19916.2655      NONANAL          0.076  0.04319.4173      METHYL NONANOATE 0.000  0.16219.5675      2-DECANONE,      0.189  0.158        A-YLANGENE19.9377      A-COPAENE        0.164  0.53021.0083      LINALOOL         0.714  0.40021.3188      A-GURJUNENE      0.000  0.69621.4289      7-METHYL-2-DECANONE                         0.076  0.07922.5693      C15H24 CALARENE? 0.109  0.18622.7295      A-BERGAMOTENE,   0.094  0.156        METHYL DECANOATE23.0196      2-UNDECANONE     0.793  0.12723.1097      2-DECANOL        0.227  0.45323.4499      8-CARYOPHYLLENE  5.394  6.43923.84102     METHYL DEC-4-ENOATE                         0.760  1.18024.03103     MW168 2-OL?      0.105  0.044        ME 43,81,110,10825.18114     B-FARNESENE      1.041  0.00026.10122     HUMULENE         17.304 14.54726.16123     METHYL DEC-4,8-  0.000  0.583        DIENOATE26.46129     WILEY# 123998 MW 204                         0.232  1.511        C15H2426.59131     2-DODECANONE     0.403  0.00027.32138     A-FARNESENE OR   0.319  0.362        ZINGIBERENE27.53139     CAS# 030021-74-0 0.099  0.33127.75143     A-MUUROLENE      0.148  0.51027.90144     B-SELINENE       2.769  0.88628.04145     A-SELINENE       3.204  0.97128.34147     METHYL           0.241  0.000        ?-UNDECENOATE29.16154     D-CADINENE       0.810  2.68529.39155     G-CADINENE       0.409  1.49330.34164     CAS# 016728-99-7 OR                         0.105  1.965        G-SELINENE30.73166     A-MUUROLINE      0.000  0.28831.15170     2-TRIDECANONE    0.397  0.51231.36171     GERANYL PROPANOATE                         0.000  0.08831.46172     MW 212 METHYL    0.000  0.074        ?-DODECENOATE32.28177     trans-GERANIOL   0.000  0.97932.54179     CAS# 000483-77-2 0.061  0.346        (CALAMENENE ?)34.38187     METHYL 3,6-      0.000  0.446        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.135  0.08939.11211     CARYOPHYLLENE OXIDE                         0.106  0.06640.23221     HUMULENE         0.048  0.131        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.232  0.12241.07225     HUMULENE         0.250  0.153        MONO-EPOXIDE II41.55228     HUMULENE         0.063  0.136        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         0.356  0.26843.88242     MW 220 ME 43,79,91                         0.163  0.00044.73246     T-CADINOL        0.077  0.24846.08255     T-MUUROLOL       0.057  0.119        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000  0.000        DIEPOXIDE?48.69268     FARNESOL         0.076  0.524______________________________________GC/          TENTATIVE IDENTI-                         65103   65103FID  CMPD.   FICATION BY GC./ CLUS-   CLUS-RT.  #       MASS SPECTROSCOPY                         TER L-1 TER L-2______________________________________6.42 9       A-PINENE         0.103   0.1537.46 14      ISOBUTYL ISOBUTYRATE                         0.280   0.1538.10 16      8-PINENE         0.866   0.6559.11 18      MYRCENE          60.712  45.6539.77 22      2-METHYL-BUTYL   0.536   0.335        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.483   0.5259.96 24      2-METHYL-BUTYL   1.641   1.178        ISOBUTARATE10.2826      L-LIMONENE       0.268   0.23510.6227      B-PHELLANDRENE?  0.295   0.210        C10H1511.5932      trans-OCIMENE    0.370   0.22912.4535      2-METHYL-BUTYL   0.075   0.070        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.244   0.22012.9140      ISOAMYL ISOVALERATE                         0.187   0.27114.3143      METHYL 4-METHYL- 0.342   0.310        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.447   0.399        HEPTANOATE15.9851      METHYL OCTANOATE 0.721   0.55516.2655      NONANAL          0.250   0.46719.4173      METHYL NONANOATE 0.165   0.15819.5675      2-DECANONE,      0.060   0.100        A-YLANGENE19.9377      A-COPAENE        0.126   0.24221.0083      LINALOOL         0.347   0.25921.3188      A-GURJUNENE      0.372   0.37421.4289      7-METHYL-2-DECANONE                         0.071   0.07522.5693      C15H24 CALARENE? 0.082   0.15822.7295      A-BERGAMOTENE,   0.953   0.855        METHYL DECANOATE23.0196      2-UNDECANONE     0.533   0.64823.1097      2-DECANOL        0.180   0.35223.4499      8-CARYOPHYLLENE  4.725   8.08023.84102     METHYL DEC-4-ENOATE                         1.801   1.92724.03103     MW168 2-OL?      0.073   0.108        ME 43,81,110,10825.18114     B-FARNESENE      0.000   0.00026.10122     HUMULENE         13.195  20.80526.16123     METHYL DEC-4,8-  0.154   0.132        DIENOATE26.46129     WILEY# 123998 MW 204                         0.306   0.538        C15H2426.59131     2-DODECANONE     0.064   0.07827.32138     A-FARNESENE OR   0.135   0.197        ZINGIBERENE27.53139     CAS# 030021-74-0 0.084   0.13727.75143     A-MUUROLENE      0.127   0.21627.90144     B-SELINENE       0.319   0.55828.04145     A-SELINENE       0.356   0.61228.34147     METHYL           0.000   0.000        ?-UNDECENOATE29.16154     D-CADINENE       0.655   1.13729.39155     G-CADINENE       0.330   0.60430.34164     CAS# 016728-99-7 OR                         0.081   0.141        G-SELINENE30.73166     A-MUUROLINE      0.059   0.11031.15170     2-TRIDECANONE    0.843   1.06031.36171     GERANYL PROPANOATE                         0.000   0.00031.46172     MW 212 METHYL    0.741   0.925        ?-DODECENOATE32.28177     trans-GERANIOL   0.703   0.44632.54179     CAS# 000483-77-2 0.000   0.108        (CALAMENENE ?)34.38187     METHYL 3,6-      0.228   0.221        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.062   0.08239.11211     CARYOPHYLLENE OXIDE                         0.094   0.25140.23221     HUMULENE         0.000   0.147        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.151   0.20141.07225     HUMULENE         0.237   0.653        MONO-EPOXIDE II41.55228     HUMULENE         0.000   0.137        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         0.666   1.03643.88242     MW 220 ME 43,79,91                         0.429   0.60944.73246     T-CADINOL        0.088   0.17046.08255     T-MUUROLOL       0.000   0.090        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000   0.077        DIEPOXIDE?48.69268     FARNESOL         0.066   0.084______________________________________GC/          TENTATIVE IDENTI-FID  CMPD.   FICATION BY GC./ 21220   21016RT.  #       MASS SPECTROSCOPY                         EROICA  FUGGLE______________________________________6.42 9       A-PINENE         0.155   0.0757.46 14      ISOBUTYL ISOBUTYRATE                         0.000   0.2218.10 16      8-PINENE         0.884   0.7129.11 18      MYRCENE          61.773  54.0399.77 22      2-METHYL-BUTYL   0.237   0.157        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.124   0.0009.96 24      2-METHYL-BUTYL   0.549   0.443        ISOBUTARATE10.2826      L-LIMONENE       0.233   0.18610.6227      B-PHELLANDRENE?  0.264   0.242        C10H1511.5932      trans-OCIMENE    1.577   0.10512.4535      2-METHYL-BUTYL   0.000   0.000        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.292   0.42612.9140      ISOAMYL ISOVALERATE                         0.000   0.00014.3143      METHYL 4-METHYL- 0.000   0.170        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.271   0.114        HEPTANOATE15.9851      METHYL OCTANOATE 0.257   0.08816.2655      NONANAL          0.070   0.09819.4173      METHYL NONANOATE 0.204   0.09719.5675      2-DECANONE,      0.161   0.212        A-YLANGENE19.9377      A-COPAENE        0.053   0.22521.0083      LINALOOL         0.508   0.65121.3188      A-GURJUNENE      0.155   0.00021.4289      7-METHYL-2-DECANONE                         0.214   0.08322.5693      C15H24 CALARENE? 0.000   0.15722.7295      A-BERGAMOTENE,   0.325   0.272        METHYL DECANOATE23.0196      2-UNDECANONE     0.820   0.40623.1097      2-DECANOL        0.000   0.34823.4499      8-CARYOPHYLLENE  12.756  7.62823.84102     METHYL DEC-4-ENOATE                         0.875   0.40924.03103     MW168 2-OL?      0.167   0.122        ME 43,81,110,10825.18114     B-FARNESENE      0.000   4.87026.10122     HUMULENE         0.355   20.05926.16123     METHYL DEC-4,8-  0.419   0.147        DIENOATE26.46129     WILEY# 123998 MW 204                         0.104   0.528        C15H2426.59131     2-DODECANONE     0.344   0.07327.32138     A-FARNESENE OR   0.000   0.543        ZINGIBERENE27.53139     CAS# 030021-74-0 0.000   0.09027.75143     A-MUUROLENE      0.060   0.17327.90144     B-SELINENE       2.268   0.17728.04145     A-SELINENE       2.562   0.18628.34147     METHYL           0.000   0.424        ?-UNDECENOATE29.16154     D-CADINENE       0.293   1.09229.39155     G-CADINENE       0.167   0.57330.34164     CAS# 016728-99-7 OR                         0.000   0.147        G-SELINENE30.73166     A-MUUROLINE      0.000   0.11731.15170     2-TRIDECANONE    0.606   0.39731.36171     GERANYL PROPANOATE                         0.091   0.00031.46172     MW 212 METHYL    0.000   0.000        ?-DODECENOATE32.28177     trans-GERANIOL   0.949   0.00032.54179     CAS# 000483-77-2 0.077   0.080        (CALAMENENE ?)34.38187     METHYL 3,6-      0.228   0.064        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.437   0.08039.11211     CARYOPHYLLENE OXIDE                         0.156   0.12640.23221     HUMULENE         0.164   0.000        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.878   0.08741.07225     HUMULENE         0.000   0.279        MONO-EPOXIDE II41.55228     HUMULENE         0.000   0.088        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         1.761   0.43543.88242     MW 220 ME 43,79,91                         0.461   0.23544.73246     T-CADINOL        0.055   0.12546.08255     T-MUUROLOL       0.000   0.070        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000   0.000        DIEPOXIDE?48.69268     FARNESOL         0.326   0.134______________________________________GC/          TENTATIVE IDENTI-        21014FID  CMPD.   FICATION BY GC./ 21102   HALL-RT.  #       MASS SPECTROSCOPY                         GALENA  ERTAU______________________________________6.42 9       A-PINENE         0.109   0.0647.46 14      ISOBUTYL ISOBUTYRATE                         0.479   0.1518.10 16      8-PINENE         0.027   0.6539.11 18      MYRCENE          55.179  53.5089.77 22      2-METHYL-BUTYL   1.009   0.184        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.554   0.0009.96 24      2-METHYL-BUTYL   5.004   0.278        ISOBUTARATE10.2826      L-LIMONENE       0.218   0.15810.6227      B-PHELLANDRENE?  0.239   0.204        C10H1511.5932      trans-OCIMENE    2.260   0.14412.4535      2-METHYL-BUTYL   0.151   0.000        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.427   0.64712.9140      ISOAMYL ISOVALERATE                         0.169   0.00014.3143      METHYL 4-METHYL- 0.152   0.264        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.930   0.150        HEPTANOATE15.9851      METHYL OCTANOATE 0.878   0.20316.2655      NONANAL          0.079   0.09519.4173      METHYL NONANOATE 0.436   0.13819.5675      2-DECANONE,      0.071   0.258        A-YLANGENE19.9377      A-COPAENE        0.111   0.21421.0083      LINALOOL         0.206   0.93921.3188      A-GURJUNENE      0.577   0.04421.4289      7-METHYL-2-DECANONE                         0.009   0.08622.5693      C15H24 CALARENE? 0.053   0.12022.7295      A-BERGAMOTENE,   1.317   0.000        METHYL DECANOATE23.0196      2-UNDECANONE     0.386   0.69623.1097      2-DECANOL        0.131   0.33723.4499      8-CARYOPHYLLENE  4.602   7.23823.84102     METHYL DEC-4-ENOATE                         2.237   0.66024.03103     MW168 2-OL?      0.042   0.157        ME 43,81,110,10825.18114     B-FARNESENE      0.000   0.00026.10122     HUMULENE         10.599  24.79926.16123     METHYL DEC-4,8-  0.180   0.000        DIENOATE26.46129     WILEY# 123998 MW 204                         0.281   0.670        C15H2426.59131     2-DODECANONE     0.076   0.13227.32138     A-FARNESENE OR   0.000   0.227        ZINGIBERENE27.53139     CAS# 030021-74-0 0.054   0.11327.75143     A-MUUROLENE      0.101   0.21427.90144     B-SELINENE       0.465   0.19028.04145     A-SELINENE       0.544   0.20728.34147     METHYL           0.000   0.069        ?-UNDECENOATE29.16154     D-CADINENE       0.586   1.25029.39155     G-CADINENE       0.300   0.65330.34164     CAS# 016728-99-7 OR                         0.142   0.151        G-SELINENE30.73166     A-MUUROLINE      0.064   0.13631.15170     2-TRIDECANONE    1.561   0.42131.36171     GERANYL PROPANOATE                         0.119   0.00031.46172     MW 212 METHYL    0.219   0.000        ?-DODECENOATE32.28177     trans-GERANIOL   0.000   0.00032.54179     CAS# 000483-77-2 0.000   0.102        (CALAMENENE ?)34.38187     METHYL 3,6-      1.725   0.071        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.186   0.12539.11211     CARYOPHYLLENE OXIDE                         0.064   0.11140.23221     HUMULENE         0.000   0.099        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.237   0.23941.07225     HUMULENE         0.134   0.357        MONO-EPOXIDE II41.55228     HUMULENE         0.000   0.100        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         0.579   0.63843.88242     MW 220 ME 43,79,91                         0.263   0.26344.73246     T-CADINOL        0.059   0.12546.08255     T-MUUROLOL       0.000   0.075        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000   0.000        DIEPOXIDE?48.69268     FARNESOL         0.000   0.064______________________________________GC/          TENTATIVE IDENTI-                         21135  21097FID  CMPD.   FICATION BY GC./ HERS-  HULLERERRT.  #       MASS SPECTROSCOPY                         BRUCK  BITTERER______________________________________6.42 9       A-PINENE         0.000  0.0877.46 14      ISOBUTYL ISOBUTYRATE                         0.000  0.0888.10 16      8-PINENE         0.527  0.7379.11 18      MYRCENE          44.828 52.8189.77 22      2-METHYL-BUTYL   0.000  0.415        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.000  0.1689.96 24      2-METHYL-BUTYL   0.066  0.844        ISOBUTARATE10.2826      L-LIMONENE       0.147  0.20710.6227      B-PHELLANDRENE?  0.145  0.212        C10H1511.5932      trans-OCIMENE    0.000  0.09212.4535      2-METHYL-BUTYL   0.000  0.079        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.418  0.23112.9140      ISOAMYL ISOVALERATE                         0.000  0.07514.3143      METHYL 4-METHYL- 0.289  0.000        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.171  0.408        HEPTANOATE15.9851      METHYL OCTANOATE 0.134  0.11016.2655      NONANAL          0.226  0.08419.4173      METHYL NONANOATE 0.090  0.08819.5675      2-DECANONE,      0.334  0.251        A-YLANGENE19.9377      A-COPAENE        0.259  0.61721.0083      LINALOOL         0.805  0.50321.3188      A-GURJUNENE      0.077  0.16921.4289      7-METHYL-2-DECANONE                         0.214  0.12122.5693      C15H24 CALARENE? 0.190  0.30022.7295      A-BERGAMOTENE,   0.000  0.000        METHYL DECANOATE23.0196      2-UNDECANONE     0.921  0.38723.1097      2-DECANOL        0.531  0.60223.4499      8-CARYOPHYLLENE  5.573  4.38623.84102     METHYL DEC-4-ENOATE                         0.551  0.77224.03103     MW168 2-OL?      0.210  0.122        ME 43,81,110,10825.18114     B-FARNESENE      0.324  0.09326.10122     HUMULENE         29.352 8.09026.16123     METHYL DEC-4,8-  0.132  0.352        DIENOATE26.46129     WILEY# 123998 MW 204                         0.735  1.994        C15H2426.59131     2-DODECANONE     0.203  0.00027.32138     A-FARNESENE OR   0.326  0.273        ZINGIBERENE27.53139     CAS# 030021-74-0 0.103  0.35227.75143     A-MUUROLENE      0.248  0.57427.90144     B-SELINENE       0.306  2.22128.04145     A-SELINENE       0.335  2.42628.34147     METHYL           0.191  0.399        ?-UNDECENOATE29.16154     D-CADINENE       1.330  2.71429.39155     G-CADINENE       0.746  1.51630.34164     CAS# 016728-99-7 OR                         0.141  3.993        G-SELINENE30.73166     A-MUUROLINE      0.146  0.36431.15170     2-TRIDECANONE    0.580  0.24131.36171     GERANYL PROPANOATE                         0.000  0.00031.46172     MW 212 METHYL    0.000  0.000        ?-DODECENOATE32.28177     trans-GERANIOL   0.000  0.13432.54179     CAS# 000483-77-2 0.229  0.205        (CALAMENENE ?)34.38187     METHYL 3,6-      0.000  0.340        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.147  0.17939.11211     CARYOPHYLLENE OXIDE                         0.331  0.08240.23221     HUMULENE         0.145  0.137        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.260  0.00041.07225     HUMULENE         0.962  0.194        MONO-EPOXIDE II41.55228     HUMULENE         0.159  0.074        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         0.752  0.53143.88242     MW 220 ME 43,79,91                         0.489  0.19544.73246     T-CADINOL        0.177  0.33646.08255     T-MUUROLOL       0.114  0.132        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000  0.000        DIEPOXIDE?48.69268     FARNESOL         0.161  0.166______________________________________GC/          TENTATIVE IDENTI-        21113FID  CMPD.   FICATION BY GC./ 21457   LUB-RT.  #       MASS SPECTROSCOPY                         LIBERTY ELSKA______________________________________6.42 9       A-PINENE         0.043   0.0477.46 14      ISOBUTYL ISOBUTYRATE                         0.068   0.0008.10 16      8-PINENE         0.504   0.4219.11 18      MYRCENE          41.506  29.5589.77 22      2-METHYL-BUTYL   0.117   0.000        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.000   0.0009.96 24      2-METHYL-BUTYL   0.197   0.176        ISOBUTARATE10.2826      L-LIMONENE       0.124   0.14510.6227      B-PHELLANDRENE?  0.149   0.099        C10H1511.5932      trans-OCIMENE    0.066   0.00012.4535      2-METHYL-BUTYL   0.000   0.000        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.293   0.25912.9140      ISOAMYL ISOVALERATE                         0.000   0.00014.3143      METHYL 4-METHYL- 0.128   0.081        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.083   0.148        HEPTANOATE15.9851      METHYL OCTANOATE 0.189   0.10016.2655      NONANAL          0.091   0.19319.4173      METHYL NONANOATE 0.092   0.18319.5675      2-DECANONE,      0.240   0.268        A-YLANGENE19.9377      A-COPAENE        0.304   0.26821.0083      LINALOOL         0.814   0.25421.3188      A-GURJUNENE      0.043   0.10621.4289      7-METHYL-2-DECANONE                         0.098   0.19822.5693      C15H24 CALARENE? 0.172   0.17922.7295      A-BERGAMOTENE,   0.000   0.947        METHYL DECANOATE23.0196      2-UNDECANONE     0.737   0.69023.1097      2-DECANOL        0.442   0.44323.4499      8-CARYOPHYLLENE  9.651   6.95323.84102     METHYL DEC-4-ENOATE                         0.663   1.08924.03103     MW168 2-OL?      0.140   0.207        ME 43,81,110,10825.18114     B-FARNESENE      0.080   17.71226.10122     HUMULENE         32.402  21.40426.16123     METHYL DEC-4,8-  0.075   0.451        DIENOATE26.46129     WILEY# 123998 MW 204                         0.387   0.796        C15H2426.59131     2-DODECANONE     0.105   0.16727.32138     A-FARNESENE OR   0.509   0.316        ZINGIBERENE27.53139     CAS# 030021-74-0 0.181   0.15727.75143     A-MUUROLENE      0.289   0.28227.90144     B-SELINENE       0.325   1.49228.04145     A-SELINENE       0.358   1.54628.34147     METHYL           0.310   0.242        ?-UNDECENOATE29.16154     D-CADINENE       1.508   1.43829.39155     G-CADINENE       0.887   0.81130.34164     CAS# 016728-99-7 OR                         0.213   0.237        G-SELINENE30.73166     A-MUUROLINE      0.103   0.15931.15170     2-TRIDECANONE    0.458   0.73831.36171     GERANYL PROPANOATE                         0.000   0.00031.46172     MW 212 METHYL    0.000   0.070        ?-DODECENOATE32.28177     trans-GERANIOL   0.099   0.25732.54179     CAS# 000483-77-2 0.193   0.196        (CALAMENENE ?)34.38187     METHYL 3,6-      0.057   0.203        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.115   0.19939.11211     CARYOPHYLLENE OXIDE                         0.205   0.55040.23221     HUMULENE         0.103   0.298        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.308   0.26141.07225     HUMULENE         0.599   1.612        MONO-EPOXIDE II41.55228     HUMULENE         0.144   0.000        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         0.568   1.35843.88242     MW 220 ME 43,79,91                         0.342   0.43944.73246     T-CADINOL        0.179   0.24746.08255     T-MUUROLOL       0.128   0.131        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000   0.094        DIEPOXIDE?48.69268     FARNESOL         0.340   0.339______________________________________                                 21073GC/          TENTATIVE IDENTI-                         21114   NORTH-FID  CMPD.   FICATION BY GC./ NADWIS- ERNRT.  #       MASS SPECTROSCOPY                         LANSKA  BREWER______________________________________6.42 9       A-PINENE         0.066   0.1257.46 14      ISOBUTYL ISOBUTYRATE                         0.000   0.1748.10 16      8-PINENE         0.649   0.8629.11 18      MYRCENE          44.179  55.5879.77 22      2-METHYL-BUTYL   0.000   0.183        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.000   0.1089.96 24      2-METHYL-BUTYL   0.000   0.428        ISOBUTARATE10.2826      L-LIMONENE       0.169   0.24810.6227      B-PHELLANDRENE?  0.149   0.265        C10H1511.5932      trans-OCIMENE    0.000   0.48812.4535      2-METHYL-BUTYL   0.000   0.000        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.391   0.70612.9140      ISOAMYL ISOVALERATE                         0.000   0.00014.3143      METHYL 4-METHYL- 0.206   0.251        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.178   0.150        HEPTANOATE15.9851      METHYL OCTANOATE 0.113   0.19916.2655      NONANAL          0.141   0.07719.4173      METHYL NONANOATE 0.155   0.16619.5675      2-DECANONE,      0.260   0.177        A-YLANGENE19.9377      A-COPAENE        0.160   0.28421.0083      LINALOOL         0.390   0.34421.3188      A-GURJUNENE      0.073   0.00021.4289      7-METHYL-2-DECANONE                         0.116   0.04922.5693      C15H24 CALARENE? 0.113   0.15422.7295      A-BERGAMOTENE,   0.854   0.000        METHYL DECANOATE23.0196      2-UNDECANONE     0.592   0.29223.1097      2-DECANOL        0.284   0.30723.4499      8-CARYOPHYLLENE  5.415   9.21723.84102     METHYL DEC-4-ENOATE                         0.939   0.56524.03103     MW168 2-OL?      0.211   0.077        ME 43,81,110,10825.18114     B-FARNESENE      17.417  0.00026.10122     HUMULENE         18.740  21.64226.16123     METHYL DEC-4,8-  0.368   0.389        DIENOATE26.46129     WILEY# 123998 MW 204                         0.461   0.619        C15H2426.59131     2-DODECANONE     0.127   0.08427.32138     A-FARNESENE OR   0.264   0.179        ZINGIBERENE27.53139     CAS# 030021-74-0 0.053   0.11227.75143     A-MUUROLENE      0.143   0.21727.90144     B-SELINENE       0.217   0.18228.04145     A-SELINENE       0.161   0.21028.34147     METHYL           0.206   0.000        ?-UNDECENOATE29.16154     D-CADINENE       0.766   1.30129.39155     G-CADINENE       0.431   0.65030.34164     CAS# 016728-99-7 OR                         0.121   0.129        G-SELINENE30.73166     A-MUUROLINE      0.085   0.12431.15170     2-TRIDECANONE    0.417   0.33731.36171     GERANYL PROPANOATE                         0.000   0.00031.46172     MW 212 METHYL    0.000   0.000        ?-DODECENOATE32.28177     trans-GERANIOL   0.200   0.00032.54179     CAS# 000483-77-2 0.131   0.110        (CALAMENENE ?)34.38187     METHYL 3,6-      0.146   0.068        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.127   0.08239.11211     CARYOPHYLLENE OXIDE                         0.284   0.12240.23221     HUMULENE         0.156   0.065        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.127   0.10941.07225     HUMULENE         0.855   0.250        MONO-EPOXIDE II41.55228     HUMULENE         0.112   0.090        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         0.755   0.29543.88242     MW 220 ME 43,79,91                         0.210   0.16944.73246     T-CADINOL        0.113   0.11946.08255     T-MUUROLOL       0.045   0.057        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000   0.000        DIEPOXIDE?48.69268     FARNESOL         0.187   0.230______________________________________GC/          TENTATIVE IDENTI-                         21044FID  CMPD.   FICATION BY GC./ NORTH-  21197RT.  #       MASS SPECTROSCOPY                         DOWN    NUGGET______________________________________6.42 9       A-PINENE         0.066   0.0957.46 14      ISOBUTYL ISOBUTYRATE                         0.000   0.2448.10 16      8-PINENE         0.639   0.6719.11 18      MYRCENE          42.280  60.6769.77 22      2-METHYL-BUTYL   0.106   0.559        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.000   0.5509.96 24      2-METHYL-BUTYL   0.173   1.307        ISOBUTARATE10.2826      L-LIMONENE       0.173   0.20310.6227      B-PHELLANDRENE?  0.199   0.244        C10H1511.5932      trans-OCIMENE    0.104   0.57112.4535      2-METHYL-BUTYL   0.000   0.063        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.337   0.61512.9140      ISOAMYL ISOVALERATE                         0.000   0.12414.3143      METHYL 4-METHYL- 0.127   0.201        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.098   0.257        HEPTANOATE15.9851      METHYL OCTANOATE 0.165   0.47016.2655      NONANAL          0.000   0.05219.4173      METHYL NONANOATE 0.122   0.32619.5675      2-DECANONE,      0.000   0.052        A-YLANGENE19.9377      A-COPAENE        0.321   0.16721.0083      LINALOOL         0.559   0.90421.3188      A-GURJUNENE      0.000   0.11321.4289      7-METHYL-2-DECANONE                         0.000   0.00022.5693      C15H24 CALARENE? 0.228   0.06922.7295      A-BERGAMOTENE,   0.000   0.298        METHYL DECANOATE23.0196      2-UNDECANONE     0.181   0.36323.1097      2-DECANOL        0.474   0.23023.4499      8-CARYOPHYLLENE  12.260  6.28923.84102     METHYL DEC-4-ENOATE                         0.798   1.36624.03103     MW168 2-OL?      0.000   0.054        ME 43,81,110,10825.18114     B-FARNESENE      0.746   0.00026.10122     HUMULENE         31.313  15.38626.16123     METHYL DEC-4,8-  0.000   0.128        DIENOATE26.46129     WILEY# 123998 MW 204                         0.198   0.359        C15H2426.59131     2-DODECANONE     0.800   0.07727.32138     A-FARNESENE OR   0.210   0.321        ZINGIBERENE27.53139     CAS# 030021-74-0 0.153   0.08627.75143     A-MUUROLENE      0.300   0.23727.90144     B-SELINENE       0.291   0.56028.04145     A-SELINENE       0.324   0.64728.34147     METHYL           0.000   0.164        ?-UNDECENOATE29.16154     D-CADINENE       1.517   0.76029.39155     G-CADINENE       0.815   0.39930.34164     CAS# 016728-99-7 OR                         0.146   0.102        G-SELINENE30.73166     A-MUUROLINE      0.163   0.07531.15170     2-TRIDECANONE    0.149   0.50031.36171     GERANYL PROPANOATE                         0.000   0.00031.46172     MW 212 METHYL    0.000   0.077        ?-DODECENOATE32.28177     trans-GERANIOL   0.000   0.00032.54179     CAS# 000483-77-2 0.199   0.000        (CALAMENENE ?)34.38187     METHYL 3,6-      0.000   0.119        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.000   0.11239.11211     CARYOPHYLLENE OXIDE                         0.410   0.05140.23221     HUMULENE         0.089   0.000        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.067   0.32841.07225     HUMULENE         0.801   0.113        MONO-EPOXIDE II41.55228     HUMULENE         0.153   0.000        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         0.168   0.64343.88242     MW 220 ME 43,79,91                         0.101   0.20644.73246     T-CADINOL        0.138   0.06246.08255     T-MUUROLOL       0.086   0.000        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000   0.000        DIEPOXIDE?48.69268     FARNESOL         0.154   0.071______________________________________GC/          TENTATIVE IDENTI-FID  CMPD.   FICATION BY GC./ 21223   21227RT.  #       MASS SPECTROSCOPY                         OLYMPIC PERLE______________________________________6.42 9       A-PINENE         0.127   0.0777.46 14      ISOBUTYL ISOBUTYRATE                         0.116   0.1938.10 16      8-PINENE         1.037   0.6189.11 18      MYRCENE          61.466  41.4899.77 22      2-METHYL-BUTYL   0.470   0.120        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.121   0.0009.96 24      2-METHYL-BUTYL   1.424   0.308        ISOBUTARATE10.2826      L-LIMONENE       0.247   0.17710.6227      B-PHELLANDRENE?  0.269   0.199        C10H1511.5932      trans-OCIMENE    0.040   0.59112.4535      2-METHYL-BUTYL   0.549   0.000        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.136   0.42512.9140      ISOAMYL ISOVALERATE                         0.476   0.00014.3143      METHYL 4-METHYL- 0.051   0.111        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.216   0.061        HEPTANOATE15.9851      METHYL OCTANOATE 0.066   0.05316.2655      NONANAL          0.053   0.09619.4173      METHYL NONANOATE 0.040   0.04719.5675      2-DECANONE,      0.056   0.256        A-YLANGENE19.9377      A-COPAENE        0.201   0.35021.0083      LINALOOL         0.316   0.24221.3188      A-GURJUNENE      0.096   0.00021.4289      7-METHYL-2-DECANONE                         0.000   0.04722.5693      C15H24 CALARENE? 0.093   0.22422.7295      A-BERGAMOTENE,   0.055   0.000        METHYL DECANOATE23.0196      2-UNDECANONE     0.000   0.31923.1097      2-DECANOL        0.238   0.48723.4499      8-CARYOPHYLLENE  8.722   12.48023.84102     METHYL DEC-4-ENOATE                         0.743   0.15024.03103     MW168 2-OL?      0.000   0.096        ME 43,81,110,10825.18114     B-FARNESENE      0.000   0.16226.10122     HUMULENE         13.614  31.39726.16123     METHYL DEC-4,8-  0.408   0.175        DIENOATE26.46129     WILEY# 123998 MW 204                         0.518   0.845        C15H2426.59131     2-DODECANONE     0.000   0.11027.32138     A-FARNESENE OR   0.177   0.296        ZINGIBERENE27.53139     CAS# 030021-74-0 0.116   0.18327.75143     A-MUUROLENE      0.248   0.31527.90144     B-SELINENE       0.738   0.27628.04145     A-SELINENE       0.764   0.32028.34147     METHYL           0.000   0.000        ?-UNDECENOATE29.16154     D-CADINENE       0.979   1.77029.39155     G-CADINENE       0.547   0.86230.34164     CAS# 016728-99-7 OR                         0.116   0.190        G-SELINENE30.73166     A-MUUROLINE      0.107   0.17731.15170     2-TRIDECANONE    0.300   0.27331.36171     GERANYL PROPANOATE                         0.105   0.00031.46172     MW 212 METHYL    0.065   0.000        ?-DODECENOATE32.28177     trans-GERANIOL   1.055   0.00032.54179     CAS# 000483-77-2 0.084   0.123        (CALAMENENE ?)34.38187     METHYL 3,6-      0.063   0.000        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.000   0.07739.11211     CARYOPHYLLENE OXIDE                         0.166   0.15240.23221     HUMULENE         0.059   0.072        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.113   0.10041.07225     HUMULENE         0.224   0.366        MONO-EPOXIDE II41.55228     HUMULENE         0.076   0.136        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         0.253   0.38943.88242     MW 220 ME 43,79,91                         0.139   0.27644.73246     T-CADINOL        0.097   0.18646.08255     T-MUUROLOL       0.057   0.115        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000   0.000        DIEPOXIDE?48.69268     FARNESOL         0.127   0.192______________________________________GC/          TENTATIVE IDENTI-                         60052FID  CMPD.   FICATION BY GC./ PRIDE OF 21077RT.  #       MASS SPECTROSCOPY                         RINGWOOD SAAZ______________________________________6.42 9       A-PINENE         0.089    0.0497.46 14      ISOBUTYL ISOBUTYRATE                         0.043    0.0008.10 16      8-PINENE         0.690    0.4869.11 18      MYRCENE          46.727   34.0509.77 22      2-METHYL-BUTYL   0.121    0.000        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.000    0.0009.96 24      2-METHYL-BUTYL   0.220    0.000        ISOBUTARATE10.2826      L-LIMONENE       0.197    0.16610.6227      B-PHELLANDRENE?  0.156    0.081        C10H1511.5932      trans-OCIMENE    0.000    0.00012.4535      2-METHYL-BUTYL   0.000    0.000        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.334    0.62612.9140      ISOAMYL ISOVALERATE                         0.000    0.00014.3143      METHYL 4-METHYL- 0.180    0.258        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.319    0.340        HEPTANOATE15.9851      METHYL OCTANOATE 0.473    0.22516.2655      NONANAL          0.116    0.30819.4173      METHYL NONANOATE 0.250    0.38019.5675      2-DECANONE,      0.147    0.501        A-YLANGENE19.9377      A-COPAENE        0.241    0.19121.0083      LINALOOL         0.317    0.33721.3188      A-GURJUNENE      0.230    0.17321.4289      7-METHYL-2-DECANONE                         0.125    0.22222.5693      C15H24 CALARENE? 0.133    0.11322.7295      A-BERGAMOTENE,   0.326    1.043        METHYL DECANOATE23.0196      2-UNDECANONE     0.690    1.39423.1097      2-DECANOL        0.226    0.29923.4499      8-CARYOPHYLLENE  8.708    5.01023.84102     METHYL DEC-4-ENOATE                         1.808    1.76924.03103     MW168 2-OL?      0.230    0.417        ME 43,81,110,10825.18114     B-FARNESENE      0.000    18.90026.10122     HUMULENE         1.946    18.11126.16123     METHYL DEC-4,8-  0.571    0.259        DIENOATE26.46129     WILEY# 123998 MW 204                         0.680    0.531        C15H2426.59131     2-DODECANONE     0.053    0.26427.32138     A-FARNESENE OR   1.932    0.255        ZINGIBERENE27.53139     CAS# 030021-74-0 0.000    0.13427.75143     A-MUUROLENE      0.000    0.18927.90144     B-SELINENE       9.372    0.25528.04145     A-SELINENE       10.128   0.17828.34147     METHYL           1.329    0.104        ?-UNDECENOATE29.16154     D-CADINENE       1.124    0.82929.39155     G-CADINENE       0.650    0.51930.34164     CAS# 016728-99-7 OR                         0.137    0.181        G-SELINENE30.73166     A-MUUROLINE      0.133    0.09931.15170     2-TRIDECANONE    0.551    0.95731.36171     GERANYL PROPANOATE                         0.000    0.00031.46172     MW 212 METHYL    0.104    0.081        ?-DODECENOATE32.28177     trans-GERANIOL   0.217    0.08032.54179     CAS# 000483-77-2 0.146    0.167        (CALAMENENE ?)34.38187     METHYL 3,6-      0.182    0.424        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.079    0.27239.11211     CARYOPHYLLENE OXIDE                         0.337    0.47640.23221     HUMULENE         0.076    0.329        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.175    0.29441.07225     HUMULENE         0.106    1.530        MONO-EPOXIDE II41.55228     HUMULENE         0.082    0.155        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         0.852    1.78943.88242     MW 220 ME 43,79,91                         0.231    0.48744.73246     T-CADINOL        0.140    0.17446.08255     T-MUUROLOL       0.067    0.099        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000    0.083        DIEPOXIDE?48.69268     FARNESOL         0.169    0.160______________________________________GC/          TENTATIVE IDENTI-        21173FID  CMPD.   FICATION BY GC./ 21186   STRISS-RT.  #       MASS SPECTROSCOPY                         SPALTER LESPALT______________________________________6.42 9       A-PINENE         0.091   0.0627.46 14      ISOBUTYL ISOBUTYRATE                         0.000   0.2028.10 16      8-PINENE         0.861   0.6349.11 18      MYRCENE          60.356  43.9779.77 22      2-METHYL-BUTYL   0.000   0.257        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.000   0.1129.96 24      2-METHYL-BUTYL   0.000   0.736        ISOBUTARATE10.2826      L-LIMONENE       0.225   0.17510.6227      B-PHELLANDRENE?  0.234   0.139        C10H1511.5932      trans-OCIMENE    0.055   0.08212.4535      2-METHYL-BUTYL   0.000   0.000        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.658   0.32612.9140      ISOAMYL ISOVALERATE                         0.000   0.00014.3143      METHYL 4-METHYL- 0.238   0.323        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.230   0.000        HEPTANOATE15.9851      METHYL OCTANOATE 0.168   0.10316.2655      NONANAL          0.166   0.25519.4173      METHYL NONANOATE 0.341   0.11919.5675      2-DECANONE,      0.313   0.216        A-YLANGENE19.9377      A-COPAENE        0.090   0.29321.0083      LINALOOL         0.557   0.64921.3188      A-GURJUNENE      0.127   0.00021.4289      7-METHYL-2-DECANONE                         0.144   0.13322.5693      C15H24 CALARENE? 0.055   0.24322.7295      A-BERGAMOTENE,   0.691   0.000        METHYL DECANOATE23.0196      2-UNDECANONE     0.612   0.38523.1097      2-DECANOL        0.179   0.50923.4499      8-CARYOPHYLLENE  2.481   10.50023.84102     METHYL DEC-4-ENOATE                         1.180   0.57024.03103     MW168 2-OL?      0.211   0.091        ME 43,81,110,10825.18114     B-FARNESENE      14.019  0.00026.10122     HUMULENE         9.083   29.25226.16123     METHYL DEC-4,8-  0.313   0.147        DIENOATE26.46129     WILEY# 123998 MW 204                         0.243   0.872        C15H2426.59131     2-DODECANONE     0.174   0.00027.32138     A-FARNESENE OR   0.179   0.131        ZINGIBERENE27.53139     CAS# 030021-74-0 0.000   0.08827.75143     A-MUUROLENE      0.137   0.23327.90144     B-SELINENE       0.130   0.23728.04145     A-SELINENE       0.069   0.28128.34147     METHYL           0.118   0.082        ?-UNDECENOATE29.16154     D-CADINENE       0.398   1.36429.39155     G-CADINENE       0.226   0.82430.34164     CAS# 016728-99-7 OR                         0.119   0.210        G-SELINENE30.73166     A-MUUROLINE      0.000   0.19631.15170     2-TRIDECANONE    0.479   0.25531.36171     GERANYL PROPANOATE                         0.000   0.00031.46172     MW 212 METHYL    0.063   0.000        ?-DODECENOATE32.28177     trans-GERANIOL   0.193   0.00032.54179     CAS# 000483-77-2 0.073   0.205        (CALAMENENE ?)34.38187     METHYL 3,6-      0.233   0.000        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.155   0.17239.11211     CARYOPHYLLENE OXIDE                         0.139   0.54040.23221     HUMULENE         0.106   0.152        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.137   0.15041.07225     HUMULENE         0.425   1.140        MONO-EPOXIDE II41.55228     HUMULENE         0.000   0.000        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         0.854   0.60943.88242     MW 220 ME 43,79,91                         0.186   0.14144.73246     T-CADINOL        0.064   0.14446.08255     T-MUUROLOL       0.000   0.092        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000   0.000        DIEPOXIDE?48.69268     FARNESOL         0.093   0.191______________________________________GC/          TENTATIVE IDENTI-FID  CMPD.   FICATION BY GC./ 21049   21112RT.  #       MASS SPECTROSCOPY                         STYRIAN TARGET______________________________________6.42 9       A-PINENE         0.089   0.1197.46 14      ISOBUTYL ISOBUTYRATE                         0.328   0.4068.10 16      8-PINENE         0.795   0.8659.11 18      MYRCENE          56.296  58.1649.77 22      2-METHYL-BUTYL   0.171   0.482        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.000   0.4619.96 24      2-METHYL-BUTYL   0.753   1.408        ISOBUTARATE10.2826      L-LIMONENE       0.207   0.22810.6227      B-PHELLANDRENE?  0.229   0.226        C10H1511.5932      trans-OCIMENE    0.057   0.36612.4535      2-METHYL-BUTYL   0.000   0.054        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.270   0.60212.9140      ISOAMYL ISOVALERATE                         0.000   0.11814.3143      METHYL 4-METHYL- 0.305   0.427        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.000   0.589        HEPTANOATE15.9851      METHYL OCTANOATE 0.000   0.44616.2655      NONANAL          0.141   0.10719.4173      METHYL NONANOATE 0.070   0.68919.5675      2-DECANONE,      0.216   0.821        A-YLANGENE19.9377      A-COPAENE        0.198   0.33621.0083      LINALOOL         0.751   0.89121.3188      A-GURJUNENE      0.000   0.00021.4289      7-METHYL-2-DECANONE                         0.094   0.40122.5693      C15H24 CALARENE? 0.166   0.00022.7295      A-BERGAMOTENE,   0.266   0.519        METHYL DECANOATE23.0196      2-UNDECANONE     0.301   1.50223.1097      2-DECANOL        0.366   0.28223.4499      8-CARYOPHYLLENE  7.269   4.95223.84102     METHYL DEC-4-ENOATE                         0.196   1.91224.03103     MW168 2-OL?      0.096   0.214        ME 43,81,110,10825.18114     B-FARNESENE      4.187   0.00026.10122     HUMULENE         20.607  10.29126.16123     METHYL DEC-4,8-  0.106   0.221        DIENOATE26.46129     WILEY# 123998 MW 204                         0.527   1.123        C15H2426.59131     2-DODECANONE     0.000   0.00027.32138     A-FARNESENE OR   0.000   0.233        ZINGIBERENE27.53139     CAS# 030021-74-0 0.000   0.16527.75143     A-MUUROLENE      0.211   0.21527.90144     B-SELINENE       0.152   0.32928.04145     A-SELINENE       0.184   0.40428.34147     METHYL           0.269   0.000        ?-UNDECENOATE29.16154     D-CADINENE       0.013   1.21529.39155     G-CADINENE       0.536   0.65330.34164     CAS# 016728-99-7 OR                         0.000   0.798        G-SELINENE30.73166     A-MUUROLINE      0.167   0.19831.15170     2-TRIDECANONE    0.228   0.97431.36171     GERANYL PROPANOATE                         0.000   0.00031.46172     MW 212 METHYL    0.000   0.114        ?-DODECENOATE32.28177     trans-GERANIOL   0.000   0.00032.54179     CAS# 000483-77-2 0.112   0.118        (CALAMENENE ?)34.38187     METHYL 3,6-      0.000   0.388        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.000   0.46039.11211     CARYOPHYLLENE OXIDE                         0.265   0.09540.23221     HUMULENE         0.000   0.536        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.000   0.00041.07225     HUMULENE         0.569   0.210        MONO-EPOXIDE II41.55228     HUMULENE         0.000   0.000        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         0.394   1.25343.88242     MW 220 ME 43,79,91                         0.119   0.26644.73246     T-CADINOL        0.119   0.11446.08255     T-MUUROLOL       0.000   0.000        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000   0.000        DIEPOXIDE?48.69268     FARNESOL         0.000   0.000______________________________________GC/          TENTATIVE IDENTI-                         21015  21041FID  CMPD.   FICATION BY GC./ TETT-  WTILAM-RT.  #       MASS SPECTROSCOPY                         NANG   EITF______________________________________6.42 9       A-PINENE         0.080  0.0787.46 14      ISOBUTYL ISOBUTYRATE                         0.000  0.2178.10 16      8-PINENE         0.753  0.7419.11 18      MYRCENE          50.137 55.4929.77 22      2-METHYL-BUTYL   0.000  0.234        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.000  0.0009.96 24      2-METHYL-BUTYL   0.000  0.507        ISOBUTARATE10.2826      L-LIMONENE       0.205  0.19110.6227      B-PHELLANDRENE?  0.147  0.251        C10H1511.5932      trans-OCIMENE    0.000  0.11412.4535      2-METHYL-BUTYL   0.000  0.000        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.509  0.53212.9140      ISOAMYL ISOVALERATE                         0.000  0.00014.3143      METHYL 4-METHYL- 0.230  0.224        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.260  0.120        HEPTANOATE15.9851      METHYL OCTANOATE 0.172  0.08716.2655      NONANAL          0.233  0.07319.4173      METHYL NONANOATE 0.228  0.07819.5675      2-DECANONE,      0.290  0.097        A-YLANGENE19.9377      A-COPAENE        0.129  0.19021.0083      LINALOOL         0.441  0.66121.3188      A-GURJUNENE      0.125  0.00021.4289      7-METHYL-2-DECANONE                         0.199  0.00022.5693      C15H24 CALARENE? 0.085  0.14122.7295      A-BERGAMOTENE,   0.873  0.380        METHYL DECANOATE23.0196      2-UNDECANONE     0.759  0.13123.1097      2-DECANOL        0.257  0.31323.4499      8-CARYOPHYLLENE  3.697  6.93423.84102     METHYL DEC-4-ENOATE                         1.220  0.28824.03103     MW168 2-OL?      0.255  0.041        ME 43,81,110,10825.18114     B-FARNESENE      16.938 7.09726.10122     HUMULENE         13.277 18.81526.16123     METHYL DEC-4,8-  0.338  0.196        DIENOATE26.46129     WILEY# 123998 MW 204                         0.321  0.472        C15H2426.59131     2-DODECANONE     0.169  0.00027.32138     A-FARNESENE OR   0.194  0.599        ZINGIBERENE27.53139     CAS# 030021-74-0 0.000  0.10627.75143     A-MUUROLENE      0.121  0.17027.90144     B-SELINENE       0.166  0.24528.04145     A-SELINENE       0.099  0.23828.34147     METHYL           0.177  0.407        ?-UNDECENOATE29.16154     D-CADINENE       0.532  0.97029.39155     G-CADINENE       0.317  0.49330.34164     CAS# 016728-99-7 OR                         0.114  0.107        G-SELINENE30.73166     A-MUUROLINE      0.059  0.09931.15170     2-TRIDECANONE    0.574  0.14231.36171     GERANYL PROPANOATE                         0.000  0.00031.46172     MW 212 METHYL    0.000  0.000        ?-DODECENOATE32.28177     trans-GERANIOL   0.157  0.05032.54179     CAS# 000483-77-2 0.131  0.071        (CALAMENENE ?)34.38187     METHYL 3,6-      0.192  0.000        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.163  0.00039.11211     CARYOPHYLLENE OXIDE                         0.282  0.09740.23221     HUMULENE         0.180  0.000        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.161  0.00041.07225     HUMULENE         0.901  0.200        MONO-EPOXIDE II41.55228     HUMULENE         0.089  0.071        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         1.033  0.11843.88242     MW 220 ME 43,79,91                         0.235  0.00044.73246     T-CADINOL        0.098  0.10446.08255     T-MUUROLOL       0.000  0.061        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000  0.000        DIEPOXIDE?48.69268     FARNESOL         0.131  0.457______________________________________GC/          TENTATIVE IDENTI-        9/17FID  CMPD.   FICATION BY GC./ 21498   COLUM-RT.  #       MASS SPECTROSCOPY                         YEOMAN  BUS______________________________________6.42 9       A-PINENE         0.121   0.1257.46 14      ISOBUTYL ISOBUTYRATE                         0.455   0.0668.10 16      8-PINENE         0.829   0.7459.11 18      MYRCENE          51.427  42.2679.77 22      2-METHYL-BUTYL   0.659   0.370        PROPANOATE9.90 23      ISOAMYL ISOBUTYRATE                         0.522   0.1849.96 24      2-METHYL-BUTYL   1.946   0.800        ISOBUTARATE10.2826      L-LIMONENE       0.237   0.23610.6227      B-PHELLANDRENE?  0.260   0.177        C10H1511.5932      trans-OCIMENE    0.159   0.16812.4535      2-METHYL-BUTYL   0.180   0.090        2-METHYL-BUTYRATE12.6836      METHYL HEPTANOATE                         0.349   0.00012.9140      ISOAMYL ISOVALERATE                         0.274   0.10614.3143      METHYL 4-METHYL- 0.139   0.000        HEXA-2-ENOATE14.3945      METHYL 6-METHYL- 0.119   0.153        HEPTANOATE15.9851      METHYL OCTANOATE 0.209   0.00016.2655      NONANAL          0.059   0.00019.4173      METHYL NONANOATE 0.070   0.00019.5675      2-DECANONE,      0.133        A-YLANGENE19.9377      A-COPAENE        0.252   0.76421.0083      LINALOOL         0.379   0.41721.3188      A-GURJUNENE      0.00021.4289      7-METHYL-2-DECANONE                         0.000   0.00022.5693      C15H24 CALARENE? 0.123   0.25222.7295      A-BERGAMOTENE,   0.000   0.000        METHYL DECANOATE23.0196      2-UNDECANONE     0.361   0.06823.1097      2-DECANOL        0.26823.4499      8-CARYOPHYLLENE  6.954   10.23623.84102     METHYL DEC-4-ENOATE                         0.692   0.45724.03103     MW168 2-OL?      0.115   0.000        ME 43,81,110,10825.18114     B-FARNESENE      0.000   0.00026.10122     HUMULENE         10.146  17.37026.16123     METHYL DEC-4,8-  0.553        DIENOATE26.46129     WILEY# 123998 MW 204                         0.439   1.992        C15H2426.59131     2-DODECANONE     0.045   0.00027.32138     A-FARNESENE OR   0.223   0.508        ZINGIBERENE27.53139     CAS# 030021-74-0 0.117   0.46427.75143     A-MUUROLENE      0.209   0.73327.90144     B-SELINENE       2.829   1.23028.04145     A-SELINENE       3.352   1.29128.34147     METHYL           0.075   0.000        ?-UNDECENOATE29.16154     D-CADINENE       1.028   3.41229.39155     G-CADINENE       0.484   2.04830.34164     CAS# 016728-99-7 OR                         0.134   2.809        G-SELINENE30.73166     A-MUUROLINE      0.095   0.36631.15170     2-TRIDECANONE    0.313   0.00031.36171     GERANYL PROPANOATE                         0.000   0.75331.46172     MW 212 METHYL    0.000   0.000        ?-DODECENOATE32.28177     trans-GERANIOL   0.363   0.18032.54179     CAS# 000483-77-2 0.069   0.381        (CALAMENENE ?)34.38187     METHYL 3,6-      0.061   0.000        DODECDIENOATE ME 7936.42196     MW 210 ME 43,54,82                         0.048   0.13539.11211     CARYOPHYLLENE OXIDE                         0.061   0.23540.23221     HUMULENE         0.059   0.109        MONO-EPOXIDE I40.28223     MW222 ME 43,82   0.07841.07225     HUMULENE         0.161   0.358        MONO-EPOXIDE II41.55228     HUMULENE         0.073   0.167        MONO-EPOXIDE III41.88231     MW 222 ME 43,79,80                         0.365   0.84743.88242     MW 220 ME 43,79,91                         0.000   0.45544.73246     T-CADINOL        0.101   0.23246.08255     T-MUUROLOL       0.062   0.111        ME 95,121,20447.50262     MW 220 ME 41,67,109                         0.000   0.112        DIEPOXIDE?48.69268     FARNESOL         0.148______________________________________
              APPENDIX 2______________________________________Detailed GLC analyses of Columbus and other U.S. high alpha______________________________________hops.                              Area %                  COLUMBUS    GALENAIdentification         Group    92-12-8-2   92-12-8-3______________________________________Apha-Pinene            0.125       0.184Beta-Pinene   1        0.943       0.883Myrcene       1        51.571      66.560Methyl butyl Isobutyrate                  0.838       3.575Limonene      4        0.288       0.350Linalool      3        0.470       0.294Caryophyllene 1        6.297       3.097Farnesene?    1        0.275       0.112Humulene      1        11.049      5.764Muurolene     4        0.768       0.309Selinene      4        1.472       0.341Geranyl Acetate         3        0.016       0.048delta Cadinene         4        1.834       0.292Gamma Cadinene         4        1.095       0.143Geranyl Isobutyrate         3        1.716       0.296Undecanone-2           1.348       0.059Geraniol      3        0.048       0.060Limonen-10-ol          0.155       0.021Caryophyllene oxide         2        0.259       0.014Humulene epoxide I         2        0.430       0.033Humulene epoxide II         2        1.450       0.068Caryolan-i-ol 2        0.120       0.006Humulene epoxide III         2        0.025       0.013Humulol       2        0.185       0.011beta Eudesmol?alpha Eudesmol?Humulenol II  2        0.103Humulene diepoxide?         2Major Hydrocarbons (1) 70.135      76.216Oxidation Products (2) 2.572       0.145Floral-Estery (3)      2.250       0.698Citrus Piney (4)       5.457       1.435Total Accounted For    80.414      78.494Humulene/Caryophyllene 1.753       1.861______________________________________                  CHINOOK     NUGGETIdentification         Group    92-12-9-1   92-12-9-2______________________________________Apha-Pinene            0.047       0.081Beta-Pinene   1        0.435       0.667Myrcene       1        28.469      51.972Methyl butyl Isobutyrate                  1.410       1.274Limonene      4        0.136       0.183Linalool      3        0.369       1.022Caryophyllene 1        9.516       8.597Farnesene?    1        0.353       0.162Humulene      1        21.157      19.029Muurolene     4        1.517       0.921Selinene      4        1.385       0.818Geranyl Acetate         3        0.210       0.044delta Cadinene         4        4.215       0.926Gamma Cadinene         4        2.686       0.445Geranyl Isobutyrate         3        3.323       0.076Undecanone-2           0.675       0.453Geraniol      3        0.155       0.079Limonen-10-ol          0.049       0.037Caryophyllene oxide         2        0.076       0.032Humulene epoxide I         2        0.326       0.220Humulene epoxide II         2        0.663       0.328Caryolan-i-ol 2        0.088       0.015Humulene epoxide III         2        0.033Humulol       2        0.379       0.040beta Eudesmol?         0.248alpha Eudesmol?        0.205Humulenol II  2        0.112       0.022Humulene diepoxide?         2        0.316Major Hydrocarbons (1) 59.924      80.427Oxidation Products (2) 1.993       0.657Floral-Estery (3)      4.057       1.221Citrus Piney (4)       9.939       3.293Total Accounted For    75.913      85.598Humulene/Caryophyllene 2.223       2.213______________________________________Area Percent ReportSample Name: 92-12-8-2Pk #  Ret Time Area        Area %                            Identification______________________________________1     7.030    3884        0.0312     7.854    1456        0.0113     8.010    1580        0.0124     9.053    8364        0.0665     9.260    10821       0.0866     9.358    15718       0.125 Alpha-Pinene7     9.800    4213        0.0338     10.297   2957        0.0239     10.543   9679        0.07710    11.097   967         0.00711    11.242   118386      0.943 Beta-Pinene12    11.904   1183        0.00913    12.476   6468547     51.571                            Myrcene14    12.685   4762        0.03815    12.746   3884        0.03116    13.064   52106       0.41517    13.204   48568       0.38718    13.274   105192      0.838 Methyl butyl Isobutyrate19    13.586   36238       0.288 Limonene20    13.797   3396        0.02721    13.938   26704       0.21222    14.149   4588        0.03623    14.333   8748        0.06924    14.520   1416        0.01125    14.602   16543       0.13126    14.879   17328       0.13827    15.087   7345        0.05828    15.212   931         0.00729    15.420   743         0.00530    15.506   3693        0.02931    15.722   3398        0.02732    15.801   10636       0.08433    16.009   5267        0.04234    16.069   2526        0.02035    16.244   25684       0.20436    16.624   3088        0.02437    16.793   10279       0.08138    16.915   3626        0.02839    17.427   1969        0.01540    17.673   67587       0.53841    18.248   2557        0.02042    18.354   1000        0.00843    18.423   691         0.00544    18.953   7042        0.05645    19.178   7410        0.05946    19.291   6033        0.04847    19.466   10779       0.08548    19.777   7142        0.05649    19.956   4956        0.03950    20.134   23743       0.18951    20.364   804         0.00652    20.742   2092        0.01653    20.877   5423        0.04354    20.984   1676        0.01355    21.043   923         0.00756    21.229   29717       0.23657    21.448   1539        0.01258    21.532   7494        0.05959    21.843   8179        0.06560    22.211   1838        0.01461    22.295   6856        0.05462    22.441   19924       0.15863    22.568   2933        0.02364    22.750   66342       0.52865    22.922   5479        0.04366    23.076   2236        0.01767    23.224   1919        0.01568    23.360   6240        0.04969    23.682   59058       0.470 Linalool70    23.938   25935       0.20671    24.171   19672       0.15672    24.285   1535        0.01273    24.432   6320        0.05074    24.542   1797        0.01475    24.816   10688       0.08576    25.028   9189        0.07377    25.276   29524       0.23578    25.457   3016        0.02479    25.597   16721       0.13380    25.737   4624        0.03681    25.858   45802       0.36582    26.177   789857      6.297 Caryophyllene83    26.267   147160      1.17384    26.483   11876       0.09485    26.654   2115        0.01686    26.951   19239       0.15387    27.128   7807        0.06288    27.262   34560       0.275 Farnesene?89    27.452   5485        0.04390    27.650   12130       0.09691    27.741   32092       0.25592    28.029   22059       0.17593    28.286   1385879     11.049                            Humulene94    28.518   174316      1.38995    28.816   1217        0.00996    29.099   46594       0.37197    29.289   65255       0.52098    29.452   50235       0.40099    29.663   101538      0.809100   29.743   96334       0.768 Muurolene101   29.922   184741      1.472 Selinene102   30.066   2003        0.016 Geranyl Acetate103   30.168   16997       0.135104   30.380   230089      1.834 delta Cadinene105   30.604   137367      1.095 Gamma Cadiene106   30.702   18692       0.149107   30.952   9850        0.078108   31.085   4796        0.038109   31.312   215300      1.716 Geranyl Isobutyrate110   31.411   169139      1.348 Undecanone-2111   31.517   26715       0.213112   31.630   77466       0.617113   31.737   5913        0.047114   31.821   6115        0.048 Geraniol115   32.106   9621        0.076116   32.224   20686       0.164117   32.501   13220       0.105118   32.620   30790       0.245119   33.031   7712        0.061120   33.254   2445        0.019121   33.462   7790        0.062122   33.722   28110       0.224123   33.960   4270        0.034124   34.081   2094        0.016125   34.192   4256        0.033126   34.312   4289        0.034127   34.372   5764        0.046128   34.483   5031        0.040129   34.565   5092        0.040131   34.901   34301       0.273132   35.009   13488       0.107133   35.126   5975        0.047134   35.299   1614        0.012135   35.487   11667       0.093136   35.854   17035       0.135 Limonen-10-ol137   36.161   2233        0.017138   36.312   32546       0.259 Caryophyllene oxide139   36.476   1067        0.008140   36.577   2462        0.019141   36.935   6949        0.055142   37.071   1073        0.008143   37.206   13125       0.104144   37.370   2081        0.016145   37.555   7101        0.056146   37.672   54047       0.430 Humulene epoxide I147   38.006   8150        0.065148   38.127   3162        0.025149   38.444   2423        0.019150   38.688   30971       0.246151   38.915   181869      1.450 Humulene epoxide II152   39.075   15063       0.120 Caryolan-1-ol153   39.222   3225        0.025 Humulene epoxide III154   39.501   7444        0.059155   39.809   1283        0.010156   39.935   1171        0.009157   40.158   5912        0.047158   40.396   1243        0.009159   40.677   52277       0.416160   40.871   8343        0.066161   41.254   6706        0.053162   41.404   23253       0.185 Humulol163   41.759   4806        0.038164   41.946   30832       0.245165   42.077   2776        0.022166   42.236   2521        0.020167   42.537   2687        0.021168   42.964   11140       0.088169   43.065   7509        0.059170   43.258   23800       0.189171   43.848   4438        0.035172   44.637   12985       0.103 Humulenol II173   45.039   5691        0.045174   45.892   3866        0.030175   46.597   1815        0.014176   47.014   2417        0.019177   50.412   1109        0.008= 1.25428E + 007______________________________________Area Percent ReportSample Name: 92-12-8-3Pk #  Ret Time Area        Area %                            Identification______________________________________1     7.034    2371        0.0222     7.855    753         0.0073     7.988    864         0.0084     8.623    706         0.0665     9.056    1684        0.0166     9.262    2429        0.0237     9.359    19367       0.184 alpha Pinene8     9.801    6546        0.0629     10.096   924         0.00810    10.318   10977       0.10411    10.547   49458       0.46912    10.920   660         0.00613    11.098   1561        0.01414    11.242   93039       0.883 beta Pinene15    11.733   1061        0.01016    11.901   3162        0.03017    12.090   1322        0.01218    12.484   6985773     66.360                            Myrcene19    12.692   7230        0.06820    12.750   5559        0.05221    13.073   155982      1.48122    13.217   83592       0.79423    13.301   376416      3.575 Methylbutyl Isobutyrate24    13.593   36853       0.350 Limoene25    13.810   1808        0.01726    13.942   30257       0.28727    14.151   2083        0.01928    14.317   15305       0.14529    14.529   2092        0.01930    14.609   73712       0.70031    14.896   239176      2.27232    15.091   5970        0.05633    15.333   699         0.00634    15.508   2556        0.02435    15.713   3795        0.03636    15.804   17078       0.16237    16.011   59153       0.56138    16.246   26755       0.25439    16.818   12944       0.12340    17.427   1728        0.01641    17.682   151069      1.43542    18.253   1689        0.01643    18.730   932         0.00844    18.953   1326        0.01245    19.192   122003      1.15946    19.329   5927        0.05647    19.481   11385       0.10848    19.791   7946        0.07549    19.952   1333        0.01250    20.134   5804        0.05551    20.727   9706        0.09252    20.879   21447       0.20353    21.054   2750        0.02654    21.227   17557       0.16655    21.769   2599        0.02456    22.298   42843       0.40757    22.437   4686        0.04458    22.494   4842        0.04659    22.739   15483       0.14760    22.234   720         0.00661    23.358   6212        0.05962    23.681   30996       0.294 Linalool63    23.937   41114       0.39064    24.168   9021        0.08565    24.789   2293        0.02166    25.025   2599        0.02467    25.343   82950       0.78868    25.449   1561        0.01469    25.596   28078       0.26670    25.830   3277        0.03171    26.073   326058      3.097 Caryophyllene72    26.267   160273      1.52273    26.482   8286        0.07874    26.695   887         0.00875    26.833   1478        0.01476    26.923   20836       0.19777    27.019   1307        0.01278    27.112   1737        0.01679    27.223   11802       0.112 Farnesene?80    27.440   2763        0.02681    27.709   6688        0.06382    28.023   24366       0.23183    28.227   606830      5.764 Humulene84    28.353   4325        0.04185    28.485   24982       0.23786    28.582   4950        0.04787    28.782   837         0.00888    29.082   8038        0.07689    29.251   9309        0.08890    29.429   7845        0.07491    29.642   32547       0.309 Muurolene92    29.716   35968       0.341 Selinene93    29.883   5145        0.048 Geranyl Acetate94    30.139   3103        0.02995    30.328   30746       0.292 delta Cadinene96    30.562   15061       0.143 gamma Cadinene97    30.780   1170        0.01198    31.068   2479        0.02399    31.144   7554        0.071100   31.383   31189       0.296 Geranyl Isobutyrate101   31.504   6271        0.059 Undecanone-2102   31.610   3590        0.034103   31.720   6384        0.060 Geraniol104   32.097   2303        0.021105   32.222   1438        0.013106   32.489   10866       0.103107   32.608   3493        0.033108   33.028   780         0.007109   33.719   42378       0.402110   33.949   697         0.006111   34.181   786         0.007112   34.478   1455        0.013113   34.754   699         0.006114   34.891   1927        0.018115   35.004   1561        0.014116   35.121   616         0.005117   35.487   19190       0.182118   35.892   2261        0.021 Limonen-10-ol119   36.302   1551        0.014 Caryophyllene oxide120   37.203   2536        0.024121   37.660   3554        0.033 Humulene epoxide I122   38.680   5605        0.053123   38.888   7257        0.068 Humulene epoxide II124   39.082   690         0.006 Caryolan-1-ol125   40.154   1386        0.013 Humulene epoxide III126   40.669   3261        0.031127   40.872   1801        0.017128   41.248   3412        0.032129   41.399   1245        0.011 Humulol130   41.937   4179        0.039131   43.258   1708        0.016Total area = 1.0527E + 007______________________________________Area Percent ReportSample Name: 92-12-9-1Pk #  Ret Time Area        Area %                            Identification______________________________________1     7.028    3603        0.0292     7.848    1142        0.0093     8.004    772         0.0064     9.048    3120        0.0255     9.253    7251        0.0606     9.351    5689        0.047 alpha Pinene7     9.794    4017        0.0338     10.302   1522        0.0129     10.538   14530       0.12010    11.090   761         0.00611    11.231   52365       0.435 beta Pinene12    11.886   700         0.00513    12.412   3424969     28.463                            Myrcene14    12.648   2525        0.02115    12.725   2971        0.02416    13.048   23622       0.19617    13.193   43283       0.35918    13.268   169688      1.410 Methylbutyl Isobutyrate19    13.576   16461       0.136 Limonene20    13.794   2031        0.01621    13.929   18137       0.15022    14.311   4573        0.03823    14.595   10186       0.08424    14.873   11403       0.09425    15.080   12395       0.10326    15.413   700         0.00527    15.501   899         0.00728    15.700   2332        0.01929    15.796   15536       0.12930    16.002   9137        0.07531    16.243   38778       0.32232    16.787   5760        0.04733    16.906   1286        0.01034    17.425   608         0.00535    17.676   114830      0.95436    18.247   625         0.00537    18.947   1621        0.01338    19.175   28172       0.23439    19.294   3311        0.02740    19.463   9984        0.08341    19.771   2700        0.02242    19.947   1731        0.01443    20.130   4426        0.03644    20.732   2604        0.02145    20.874   10809       0.08946    21.044   2047        0.01747    21.230   61271       0.50948    21.533   14424       0.11949    21.780   1521        0.01250    21.843   956         0.00751    22.211   2063        0.01752    22.293   15179       0.12653    22.443   32264       0.26854    22.570   3366        0.02855    22.756   106737      0.88756    23.080   5302        0.04457    23.356   6309        0.05258    23.680   44440       0.369 Linalool59    23.941   75567       0.62860    24.170   15675       0.13061    24.434   2150        0.01762    24.524   1616        0.01363    24.806   7293        0.06064    25.028   2541        0.02165    25.278   37278       0.30966    25.346   31734       0.26367    25.605   32557       0.27068    25.881   90397       0.75169    26.140   1145130     9.516 Caryophyllene70    26.269   176130      1.46371    26.486   9543        0.07972    26.835   1812        0.01573    26.988   22481       0.18674    27.140   17473       0.14575    27.353   42477       0.353 Farnesene?76    27.473   5304        0.04477    27.783   61915       0.51478    28.035   24287       0.20179    28.343   2545867     21.157                            Humulene80    28.547   315497      2.62281    28.832   2334        0.01982    28.945   5985        0.04983    29.120   75042       0.62384    29.300   116317      0.96685    29.469   97160       0.80786    29.682   182550      1.517 Muurolene87    29.764   166698      1.385 Selinene88    29.967   25357       0.210 Geranyl Acetate89    30.169   7062        0.05890    30.417   507237      4.215 delta Cadiene91    30.630   323297      2.686 gamma Cadiene92    30.879   5404        0.04493    31.069   10371       0.08694    31.155   5319        0.04495    31.332   399851      3.323 Geranyl Isoburyrate96    31.467   81272       0.675 Undecanone-297    31.617   7782        0.06498    31.730   18703       0.155 Geraniol99    31.890   7540        0.062100   32.109   8984        0.074101   32.223   86628       0.719102   32.436   9265        0.077103   32.491   6647        0.055104   32.620   30270       0.251105   33.035   3306        0.027106   33.230   743         0.006107   33.464   4214        0.035108   33.728   87090       0.723109   33.951   2444        0.020110   34.183   3253        0.027111   34.370   4703        0.039112   34.480   4897        0.040113   34.758   3342        0.027114   34.896   11595       0.096115   35.007   14221       0.118116   35.299   1278        0.010117   35.489   39880       0.331118   35.873   5944        0.049 Limonen-10-ol119   35.982   1196        0.009120   36.163   3016        0.025121   36.310   9199        0.076 Caryophyllene oxide122   36.564   1474        0.012123   36.871   5580        0.046124   37.205   13593       0.113125   37.365   21562       0.179126   37.551   3095        0.025127   37.669   39322       0.326 Humulene epoxide I128   37.863   1467        0.012129   38.004   7647        0.063130   38.124   2074        0.017131   38.438   1793        0.014132   38.685   50800       0.422133   38.900   79889       0.663 Humulene epoxide II134   39.082   10671       0.088 Caryolan-1-ol135   39.209   4042        0.033 Humulene epoxide III136   39.500   2852        0.023137   39.716   694         0.005138   39.789   600         0.005139   40.149   3072        0.025140   40.465   1310        0.010141   40.672   16151       0.134142   40.874   13029       0.108143   41.250   10136       0.084144   41.406   45643       0.379 Humulol145   41.758   11528       0.095146   41.846   13183       0.109147   41.945   25814       0.214148   42.073   9818        0.081149   42.534   5222        0.043150   42.964   29878       0.248 beta Eudesmol?151   43.065   24765       0.205 alpha Eudesmol?152   43.255   33781       0.280153   43.565   1828        0.015154   43.846   10003       0.083155   44.289   5485        0.045156   44.370   3097        0.025157   44.633   13527       0.112 Humulenol II158   45.042   12525       0.104159   45.700   38097       0.316 Humulene diepoxide?160   45.885   3830        0.031161   46.615   2140        0.017162   47.030   2967        0.024163   48.254   4136        0.034164   48.524   1729        0.014165   52.198   1275        0.010Total area = 1.20327E + 007______________________________________Area Percent ReportSample Name: 92-12-9-2Pk #  Ret Time Area        Area %                            Identification______________________________________1     7.034    1627        0.0132     7.850    1222        0.0103     9.051    1362        0.0114     9.259    2214        0.0185     9.355    9693        0.081 alpha Pinene6     9.796    3741        0.0317     10.309   1970        0.0168     10.542   19685       0.1669     11.093   943         0.00810    11.236   79174       0.667 beta Pinene11    11.895   2296        0.01912    12.468   6160576     51.972                            Myrcene13    12.679   3749        0.03114    12.741   4270        0.03615    13.058   44822       0.37816    13.201   59258       0.49917    13.275   151104      1.274 Methylbutyl Isobutyrate18    13.582   21713       0.183 Limonene19    13.800   1814        0.01520    13.934   28562       0.24121    14.144   933         0.00722    14.311   6730        0.05623    14.519   2088        0.01724    14.609   6243        0.05225    14.875   55333       0.46626    15.083   3970        0.03327    15.326   1045        0.00828    15.416   636         0.00529    15.503   2028        0.01730    15.702   2642        0.02231    15.798   10210       0.08632    16.005   54321       0.45833    16.241   21435       0.18034    16.809   7970        0.06735    17.438   1939        0.01636    17.655   61020       0.51437    17.783   1252        0.01038    18.248   665         0.00539    18.948   1086        0.00940    19.179   60530       0.51041    19.314   1798        0.01542    19.462   8464        0.07143    19.546   4332        0.03644    19.785   6451        0.05445    19.949   1390        0.01146    20.130   4840        0.04047    20.722   11806       0.09948    20.873   1283        0.01049    20.945   2757        0.02350    21.226   4405        0.03751    21.524   2418        0.02052    21.776   2520        0.02153    22.199   1123        0.00954    22.294   37518       0.31655    22.438   12669       0.10656    22.558   1478        0.01257    22.743   43371       0.36558    23.076   1222        0.01059    23.236   1059        0.00860    23.355   8797        0.07461    23.681   121220      1.022 Linalool62    23.935   17913       0.15163    24.167   9930        0.08364    24.417   1477        0.01265    24.798   946         0.00866    24.923   911         0.00767    25.023   2303        0.01968    25.347   50189       0.42369    25.462   730         0.00670    25.605   63484       0.53571    25.731   14566       0.12272    25.863   19067       0.16073    26.129   1019106     8.597 Caryophyllene74    26.267   172193      1.45275    26.484   13509       0.11476    26.649   3488        0.02977    26.830   1518        0.01278    26.973   2085        0.01779    37.057   3159        0.02680    27.127   4597        0.03881    27.284   19285       0.162 Farnesene?82    27.452   4597        0.03883    27.558   1014        0.00884    27.766   17735       0.14985    28.030   20185       0.17086    28.324   2255638     19.029                            Humulene87    28.509   62699       0.52988    28.575   78837       0.66589    28.933   16176       0.13690    29.101   26411       0.22291    29.319   43633       0.36892    29.442   23012       0.19493    29.658   109275      0.921 Muurolene94    29.741   97039       0.818 Selinene95    29.881   5248        0.044 Geranyl Acetate96    30.142   4602        0.03897    30.351   109776      0.926 delta Cadinene98    30.580   52832       0.445 gamma Cadinene99    30.771   1506        0.012100   30.851   1737        0.014101   31.082   5775        0.048102   31.205   11532       0.097103   31.330   9041        0.076 Geranyl isobutyrate104   31.511   53799       0.453 Undecanone-2105   31.717   9415        0.079 Geraniol106   31.814   3048        0.025107   32.085   5681        0.047108   32.219   5853        0.049109   32.492   1416        0.011110   32.608   7265        0.061111   33.033   906         0.007112   33.253   722         0.006113   33.712   10903       0.092114   33.945   1308        0.011115   34.071   2438        0.020116   34.181   1069        0.009117   34.368   5360        0.045118   34.474   674         0.005119   34.889   9616        0.081120   35.001   4520        0.038121   35.481   8543        0.072122   35.853   4382        0.037 Limonen 10-ol123   35.976   1266        0.010124   36.154   1163        0.009125   36.296   3788        0.032 Caryophyllene oxide126   36.909   1692        0.014127   37.183   9203        0.077128   37.358   1754        0.014129   37.660   26083       0.220 Humulene epoxide I130   37.996   2405        0.020131   38.673   14395       0.121132   38.889   38989       0.328 Humulene epoxide II133   39.079   1867        0.015 Caryolan-1 ol134   39.922   885         0.007135   40.138   1296        0.010136   40.666   11481       0.096137   40.870   4099        0.034138   41.245   7744        0.065139   41.397   4776        0.040 Humulol140   41.934   9925        0.083141   43.059   2394        0.020142   43.254   5249        0.044143   43.842   1927        0.016144   44.637   2619        0.022 Humulenol II= 1.18535E + 007______________________________________Reference:  Code     Variety  92-12-8-2           Columbus  92-12-8-3           Galena  92-12-9-1           Chinook  92-12-9-2           Nugget Analytical Conditions: See, Nickerson and van Engel, "Hop Aroma Component Profile and the Aroma Unit," Journal American Society of Brewing Chemists 50(3), 7782(1992).

Claims (1)

What is claimed is:
1. A new and distinct variety of hop plant named Columbus, substantially as shown and described.
US08/409,908 1993-11-30 1995-03-22 Hop variety named `Columbus` Expired - Lifetime USPP10956P (en)

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USPP13132P2 (en) 1999-09-09 2002-10-29 John I. Haas, Inc. Hop plant named ‘Millennium-44’
USPP13128P2 (en) 1999-09-09 2002-10-29 John I. Haas, Inc. Hop plant named ‘Millennium-48’
USPP13129P2 (en) 1999-09-09 2002-10-29 John I. Haas, Inc. Hop plant named ‘Millennium-MiddleLate’
USPP13311P2 (en) 1999-09-09 2002-12-03 John I. Haas, Inc. Hop plant named ‘Millennium-Late’
USPP14127P2 (en) * 2000-02-03 2003-09-02 Paul A. Gamache Hop plant named “VGXP01”
USPP24125P3 (en) * 2011-05-20 2013-12-31 Hop Breeding Company, LLC. Hop plant named ‘HBC 369’

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USPP8812P (en) * 1992-12-17 1994-06-28 John I. Haas, Inc. Hop plant named H87311-3
USPP8824P (en) * 1992-12-17 1994-07-05 John I. Haas, Inc. Hop plant named 37 H87203-1"
USPP8823P (en) * 1992-12-17 1994-07-05 John I. Haas, Inc. Hop plant named "H87207-2"

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USPP8812P (en) * 1992-12-17 1994-06-28 John I. Haas, Inc. Hop plant named H87311-3
USPP8824P (en) * 1992-12-17 1994-07-05 John I. Haas, Inc. Hop plant named 37 H87203-1"
USPP8823P (en) * 1992-12-17 1994-07-05 John I. Haas, Inc. Hop plant named "H87207-2"

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American Society of Brewing Chemists, Acids and Acids in Hops and Hop Extracts by HPLC (International Method), in Methods of Analysis, The Society, St. Paul, MN, 8th ed., 1992. *
Defendant s First Motion for Partial Summary Judgment, dated Jun. 9, 1997. *
Defendant's First Motion for Partial Summary Judgment, dated Jun. 9, 1997.
Defendants LR 56.1(a) Statement of Material Facts for Defendants First Partial Summary Judgment Motion, dated Jun. 9, 1997 (without exhibits). *
Defendants' LR 56.1(a) Statement of Material Facts for Defendants' First Partial Summary Judgment Motion, dated Jun. 9, 1997 (without exhibits).
Defendants Memorandum of Points and Authorities in Support of Their First Motion for Partial Summary Judgement, dated Jun. 9, 1997. *
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Hop Growers of America, Inc., 1991 1992 Annual Statistical Report, 37th Annual HGA Convention, Portland, Oregon, 1993. *
Hop Growers of America, Inc., 1991-1992 Annual Statistical Report, 37th Annual HGA Convention, Portland, Oregon, 1993.
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HopUnion's Response in Opposition to Defendants' Motion for Partial Summary Judgment, dated Jul. 8, 1997 (without exhibits).
Kenny, Identification of U.S. Grown Hop Cultivars by Hop Acid and Essential Oil Analyses, J. Amer. Soc. Brewing Chemists 48 (1):3 8, 1990. *
Kenny, Identification of U.S. Grown Hop Cultivars by Hop Acid and Essential Oil Analyses, J. Amer. Soc. Brewing Chemists 48(1):3-8, 1990.
Lewis, "The HopUnion U.S.A., Inc.--Low Trellis Hop Production System," Proc. Tech. Comm. of the IHGC of the XXXVIIIth International Hop Congress, Hereford, U.K. Published Rijksstation voor Landbouwtechniek, Merelbeke/Gent, Belgium, 17-37, 1990.
Lewis, The HopUnion U.S.A., Inc. Low Trellis Hop Production System, Proc. Tech. Comm. of the IHGC of the XXXVIIIth International Hop Congress, Hereford, U.K. Published Rijksstation voor Landbouwtechniek, Merelbeke/Gent, Belgium, 17 37, 1990. *
Nickerson and van Engel, "Hop Aroma Component Profile and the Aroma Unit," Journal of American Society of Brewing Chemists 50(3), 77-82, 1992.
Nickerson and van Engel, Hop Aroma Component Profile and the Aroma Unit, Journal of American Society of Brewing Chemists 50 (3), 77 82, 1992. *
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Peacock and McCarty, Varietal Identification of Hops and Hop Pellets, Master Brewer Assoc. of the Americas Technical Quarterly 27 :81 85, 1992. *
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Plaintiff's Opposition to Defendants' First Motion for Partial Summary Judgment Motion, dated Jun. 25, 1997.
Plaintiff's Statement of Specific Facts in Opposition to Defendants' Motion for Partial Summary Judgment, dated Jun. 25, 1997 (without exhibits) (information subject to protective order redacted).

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USPP13132P2 (en) 1999-09-09 2002-10-29 John I. Haas, Inc. Hop plant named ‘Millennium-44’
USPP13128P2 (en) 1999-09-09 2002-10-29 John I. Haas, Inc. Hop plant named ‘Millennium-48’
USPP13129P2 (en) 1999-09-09 2002-10-29 John I. Haas, Inc. Hop plant named ‘Millennium-MiddleLate’
USPP13311P2 (en) 1999-09-09 2002-12-03 John I. Haas, Inc. Hop plant named ‘Millennium-Late’
USPP14127P2 (en) * 2000-02-03 2003-09-02 Paul A. Gamache Hop plant named “VGXP01”
USPP24125P3 (en) * 2011-05-20 2013-12-31 Hop Breeding Company, LLC. Hop plant named ‘HBC 369’

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