USH2268H1 - Microtiter plate to mitigate cell distribution bias from meniscus edge - Google Patents

Microtiter plate to mitigate cell distribution bias from meniscus edge Download PDF

Info

Publication number
USH2268H1
USH2268H1 US12322958 US32295809A USH2268H1 US H2268 H1 USH2268 H1 US H2268H1 US 12322958 US12322958 US 12322958 US 32295809 A US32295809 A US 32295809A US H2268 H1 USH2268 H1 US H2268H1
Authority
US
Grant status
Grant
Patent type
Prior art keywords
plate
well
lid
liquid
meniscus
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US12322958
Other versions
US20100197004A1 (en )
Inventor
Bradford W. Gutting
Ryan S. Mackie
George A. Andrews, Jr.
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NAVY United States, REPRESENTED BY SECRETARY
US Secretary of Navy
Original Assignee
US Secretary of Navy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Grant date

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5085Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
    • B01L3/50853Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates with covers or lids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/12Well or multiwell plates
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02Adapting objects or devices to another
    • B01L2200/025Align devices or objects to ensure defined positions relative to each other
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/046Function or devices integrated in the closure
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0829Multi-well plates; Microtitration plates

Abstract

A lid assembly is provided for mitigation or removal of meniscus along the periphery of sample liquid in the cavity wells of a microtiter plate. The assembly includes a lid plate having a mount surface, an array of plugs corresponding to the array of wells, and a plurality of posts. Each plug extends below from the mount surface and is insertable into the periphery of a counterpart well for contact with the liquid. The plurality of posts suspends the lid plate above the microtiter plate. Each post optionally passes through an orifice through the mount surface, with each post including an adjustable clamp to support the lid plate. The mount surface optionally includes an array of cavities that correspond in disposition to the array of plugs. Each plug is independently insertable through the mount surface to adjust depth of each plug into its counterpart well.

Description

STATEMENT OF GOVERNMENT INTEREST

The invention described was made in the performance of official duties by one or more employees of the Department of the Navy, and thus, the invention herein may be manufactured, used or licensed by or for the Government of the United States of America for governmental purposes without the payment of any royalties thereon or therefor.

BACKGROUND

The invention relates generally to meniscus removal or mitigation in cavity wells of a microtiter plate for improved homogeneity of biological cell distribution. In particular, the invention provides devices to suppress or redistribute surface tension effects of the liquid contained in the wells.

Multiwell or microtiter plates, are ubiquitous in biological and pharmaceutical research. A microtiter plate (also known as “microplate”) represents a flat plate with multiple uniform “wells” used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories.

SUMMARY

Conventional wells in a microtiter plate yield disadvantages addressed by various exemplary embodiments of the present invention. In particular, various exemplary embodiments provide a lid assembly for superposition above the microtiter plate to mitigate or remove the meniscus along the periphery of sample liquid in the cavity wells of a microtiter plate. The assembly includes a lid plate having a mount surface, an array of plugs corresponding to the array of wells, and a plurality of posts.

In various exemplary embodiments, each plug extends below from the mount surface and is insertable into the periphery of a counterpart well for contact with the liquid. The plurality of posts suspends the lid plate above the microtiter plate. In various exemplary embodiments, each post optionally passes through an orifice through the mount surface, with each post including an adjustable clamp to support the lid plate. In alternate exemplary embodiments, the mount surface optionally includes an array of cavities that correspond in disposition to the array of plugs. Each plug is independently insertable through the mount surface to adjust depth of each plug into its counterpart well.

BRIEF DESCRIPTION OF THE DRAWINGS

These and various other features and aspects of various exemplary embodiments will be readily understood with reference to the following detailed description taken in conjunction with the accompanying drawings, in which like or similar numbers are used throughout, and in which:

FIG. 1 is an isometric view of a multiwell microtiter plate;

FIG. 2 is a detail elevation view of a well in a microtiter plate;

FIG. 3 is a detail plan view of a well with macrophage cells;

FIG. 4A is an elevation view of a well lacking meniscus mitigation;

FIGS. 4B and 4C are isometric views of wells with meniscus mitigation;

FIG. 5 is a first isometric view of a microtiter plate with a lid plate having fixed plugs;

FIG. 6 is a second isometric view of the microtiter and lid plates;

FIG. 7 is an isometric view of a microtiter plate with a lid plate having adjustable plugs;

FIG. 8A is a detail plan view of a well showing cell distribution without meniscus mitigation; and

FIG. 8B is a detail plan view of a well showing cell distribution with meniscus mitigation.

DETAILED DESCRIPTION

In the following detailed description of exemplary embodiments of the invention, reference is made to the accompanying drawings that form a part hereof, and in which is shown by way of illustration specific exemplary embodiments in which the invention may be practiced. These embodiments are described in sufficient detail to enable those skilled in the art to practice the invention. Other embodiments may be utilized, and logical, mechanical, and other changes may be made without departing from the spirit or scope of the present invention. The following detailed description is, therefore, not to be taken in a limiting sense, and the scope of the present invention is defined only by the appended claims.

FIG. 1 presents an isometric view 100 of a generic 96-well micro- titer plate 110 supported on a base 120. The microplate 110 features an array with eight columns and twelve rows of cavity wells 130. Each well 130 of this plate array holds a working volume of 300 μL fillable through the opening 140 with a liquid solution containing biological particulates, such as mammalian cells, bacteria, viruses, proteins, etc. These suspensions are initially prepared to be homogeneous via mixing before injection into the well 130 of the plate 110.

Such devises allow researchers to perform optical and spectroscopic analysis on biological samples by submersing them within various fluidic environments. Accordingly, maximal control of the sedimentation process is desirable so as to provide as much uniformity as possible both within individual wells and throughout the entire plate.

FIG. 2 shows an elevation view photograph 200 of a circular well 210 partially filled by liquid 220 with the remaining volume above containing air 230. The liquid represents the solution containing sample particulates for analysis. An interface surface 240 separates the boundaries of the liquid 220 and air 230. Additionally, the liquid 220 contacts the solid-wall boundary at the lateral and bottom peripheries of the well 210.

Due to surface tension of the liquid 220, the interface 240 exhibits curvature between the lateral periphery and the center of the well 210 for typical sample sizes. The curvature can be concave or convex depending on the contact angle between the liquid and the peripheral boundary. This phenomenon is most pronounced by the meniscus rise 250 along the edges adjacent the boundary of the well 210, thereby producing a concave curvature.

FIG. 3 shows a plan view detail photograph 300 of liquid contents of the well 210 containing the solution with J77A.4 macrophage cells 310 suspended therein. A circular periphery 320 bounds the well with an edge 330 at which meniscus forms. A directional vector 340 denotes the cell gradient from the well's peripheral edge 330 with higher cell density region 350 toward its center with lower cell density region 360. A proximal circular sample area 370 exhibits at least forty cells 310, whereas by contrast a distal circular sample 380 reveals fewer than ten cells 310.

Non-uniform sedimentation of cells 310 yields a survival consequence such that those that settle within the meniscus edge 330 region (with many neighbors) survive (as indicated by lighter shade), whereas those in the other regions 350 and 360 toward the center (with few neighbors) perish (as denoted by darker shade).

FIGS. 4A, 4B and 4C present isometric diagrams 400 of well configurations. FIG. 4A shows an unmodified well 410 with a circular periphery 420 to contain a liquid 430 bounded by a surface meniscus 435. Within the liquid 430 are macrophage cells 440, which in the well 410 remain clustered near the periphery 420.

FIG. 4B shows an exemplary embodiment of a modified well 450 with a cylindrical section 460 that segregates a center core chamber 465 and a conical bevel 470 that segregates a peripheral annular chamber 475. Both chambers 465 and 475 can contain the liquid 430. In the modified well 450, the meniscus 435 distributes over a larger area in the annular chamber 475, thereby reducing its average curvature, especially toward the well's core chamber 465.

This technique can be labeled as a beveled-well meniscus-reduction microplate to reduce the degree of meniscus curvature by confining the outer periphery that adjoins a boundary to an annular bevel portion. The liquid surface spans across a wider extent within the bevel 470, thereby flattening the surface 435 within the section 460. The reduced curvature of the liquid surface homogenizes cell distribution within the well 450.

FIG. 4C shows another exemplary embodiment of a modified well 480 featuring a lid plug 490 that negates the meniscus by providing a solid fixed surface 495 onto which surface tension forms a flat profile. Both of these exemplary wells 450 and 480 yield more uniform distribution of cells 440 as a consequence of meniscus mitigation.

This technique can be labeled as a meniscus-suppression lid applicable for either fixed or variable liquid volume. The lid employs a plug 490 that protrudes into the well 480. The plug's terminating surface 495 contacts the surface of the liquid 430 contained in the well 480, thereby removing the meniscus curvature. Special coatings can be employed on the surface 495 to inhibit material of the liquid 430 from adhering to the plug 490. Typical microtiter plates (having arrays of 6, 12, 24, 48, 96 and 384 wells) can remain unmodified for this embodiment. Instead, a researcher merely obtains lid inserts to use with commercially-available microplates.

FIG. 5 illustrates an isometric view 500 of an exemplary plate with accompanying lid. A microplate 510 includes an array of cavity wells 520 (open at their tops), each well containing a uniform volume of liquid 530. A lid plate 540 having a mount surface 550 is superpositioned above the microplate 510. An array of plugs 560 extend below the surface 550.

These plugs 560 correspond to and are disposed above the wells 520 to suppress meniscus formation in their liquid contents 530. A set of displacement posts 570 provide support adjacent the outer corners of the lid plate 540. Each post 570 includes a translatable clamp 580 to adjust the position of the lid plate 540 from the microplate 510 or its support platform.. The combination of lid plate 540, plugs 560, posts 570 and clamp 580 represent a lid assembly 590 to retrofit with a conventional plate 520.

Each plug 560 insertably fits into its corresponding well 520. For circular geometries, the plug's outer diameter is therefore less than the well's inner diameter. Preferably, the plug's outer diameter is only slightly smaller than the well's inner diameter to minimize meniscus effects.

FIG. 6 shows an isometric view 600 of the exemplary plate with the accompanying lid assembly 590 for equal volume content in the wells 520. A bracketing tray 610 (optionally adjustable) for the microplate 510 provides a platform for the posts 570. The lid plate 540 aligns to the posts 570 along coaxial lines 620. Each post 570 passes through a corresponding orifice 630 in the lid plate 540.

The clamps 580 support the lid plate 540 along their corresponding posts 570 to be disposed above the microplate 510. The clamps 580 can be adjusted to enable the plugs 560 to be disposed within their corresponding wells 520, thereby suppressing meniscus formation within their liquid contents 530.

FIG. 7 illustrates an isometric view 700 of an exemplary plate with an accompanying lid in an alternate embodiment. A microplate 510 includes an array of wells 520 (open at their tops), each well containing a volume of liquid 710 that varies from well to well. A lid plate 720 is suspended above the micro- plate 510. The lid plate 720 includes an array of plugs 730 that corresponds to the wells 520.

Each plug 730 can be vertically adjusted relative to the surface of the lid plate 720. A series of support columns 740 extend below the lid plate 720. The combination of lid plate 720, plugs 730 and columns 740 represent a lid assembly 750 to retrofit with a conventional plate 510. The columns 740 engage the microplate 510 in gaps between adjacent wells 520 to suspend the lid plate 710 above the microplate 510. The disposition of plug 730 extending from the lid plate 720 is tailored to descend into its corresponding well 520 to that specific depth so as to suppress the meniscus in that liquid content 710.

FIGS. 8A and 8B show plan view detail photographs of liquid contents of a well containing liquid and macrophage cells suspended therein. In particular, FIG. 8A presents a photograph 800 identifying a circular wall 810 of the well and a vector 820 leading to its center. Conditions for FIG. 8A are substantially analogous to those displayed in FIG. 3 without meniscus mitigation.

Near the wall 810, proximate cells 830 cluster together in greater density than distal cells 840 towards the center. By contrast, FIG. 8B demonstrates cell distribution effect from meniscus mitigation in a photograph 850, also showing the wall 810. Distribution of cells 860 exhibits considerable uniformity along the vector 820.

By suppressing meniscus formation in well liquid, cell distribution uniformity can be augmented. This can be accomplished by engaging a lid plug 480 against the liquid at its top surface. Alternatively, this can also be accomplished by providing radially segregated chambers at the liquid surface. These chambers can be bounded by an upper cylinder 460 for the cells under evaluation and a bevel cone 470 for diverting the meniscus by its extension.

The efforts leading to the described embodiments are directed to providing tissue culture plates that mitigate differential stacking of cells towards the well's periphery. The photograph 300 illustrates effects of cell stacking. In addition to there being more cells on the perimeter 310 of the well 210, the cells 320 towards the middle are dead as indicated by the stain from the darkening (blue) dye.

These untreated cells 320 were seeded in the well 210, washed, and deposited in the incubator for an interval. This heterogeneous pattern also extends to treated cells. Various exemplary embodiments present techniques to distribute the cells 320 homogeneously on the bottom of the well 210 in the plate 110. The principle options include modifying the well to expand the meniscus and incorporating a lid to conform the meniscus to a flat solid surface.

For a multiwell microplate in which each well 520 contains equal volumes, the configuration of the lid assembly 590 shown in views 500 and 600 is appropriate. This application, in which overall plate volume changes are required for different experiments, employs the lid 540 for constant-volume meniscus removal with fixed plugs 560. Each plug 560 has the same dimension and extension from the lid surface 550 being permanently attached thereto.

Adjustability for different overall meniscus heights for different experiments can be achieved by sliding the entire lid 540 upon the posts 570 that protrude through orifices 630 in the lid 540 and include adjustably translatable clamps 580 capable of supporting the lid's weight at a desired height. To avoid splay, the posts 570 can optionally interface with the bracketing tray 610, which can be designed for adaptability to enclose standard microplates 510 of various sizes or else be rigid for a fixed configuration.

For a multiwell plate in which each well 710 contains a different volume, the configuration of the lid assembly 750 shown in view 700 can be implemented. In this application, the lid plate 720 has an array of cavities, and each plug 730 individually slides through its corresponding cavity, such as by pushing with a finger. The lid plate 720 has support columns 740 that remain fixed in position to provide a constant separation from the microplate 510 enabling for maximum penetration of any particular plug 730 to the bottom of any given well 710.

The dimensions of the extruding plugs 560 and 730 need not fill the entire corresponding well 520 and 710. Although such variation might affect the meniscus response, plugs narrower than the inner region of the well may exhibit advantages in production cost and reduced surface interaction. Additionally, a single plug may be replaced with multiple smaller plugs whose adjustability can be individually customized for either the lid plate 720 or within a sub-plug platform inserted in lieu of the plug 730.

While certain features of the embodiments of the invention have been illustrated as described herein, many modifications, substitutions, changes and equivalents will now occur to those skilled in the art. It is, therefore, to be understood that the appended claims are intended to cover all such modifications and changes as fall within the true spirit of the embodiments.

Claims (3)

What is claimed is:
1. A microtiter plate for containing sample liquid, comprising;
a base platform;
a sample plate disposed on said platform; and
an array of cavity wells disposed on said sample plate, each well having a periphery for containment of the liqud, wherein
said periphery includes:
a cylindrical chamber extending upward from said platform to an intermediate height between said platform and a terminus height, and
a conic bevel chamber extending upward and radially outward from said cylindrical chamber from said intermediate height to said terminus height, wherein said well can contain the liquid in both said cylindrical and bevel chambers.
2. The microtiter plate according to claim 1, wherein said each well has a flat bottom bounded by said platform.
3. The microtiter plate according to claim 1, further including a lid plate having an array of plugs, each plug corresponding to said each well.
US12322958 2009-01-30 2009-01-30 Microtiter plate to mitigate cell distribution bias from meniscus edge Abandoned USH2268H1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US12322958 USH2268H1 (en) 2009-01-30 2009-01-30 Microtiter plate to mitigate cell distribution bias from meniscus edge

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12322958 USH2268H1 (en) 2009-01-30 2009-01-30 Microtiter plate to mitigate cell distribution bias from meniscus edge
PCT/US2010/000263 WO2010087987A1 (en) 2009-01-30 2010-01-29 Microtiter plate to mitigate cell distribution bias from meniscus edge

Publications (2)

Publication Number Publication Date
US20100197004A1 true US20100197004A1 (en) 2010-08-05
USH2268H1 true USH2268H1 (en) 2012-04-03

Family

ID=42395942

Family Applications (1)

Application Number Title Priority Date Filing Date
US12322958 Abandoned USH2268H1 (en) 2009-01-30 2009-01-30 Microtiter plate to mitigate cell distribution bias from meniscus edge

Country Status (2)

Country Link
US (1) USH2268H1 (en)
WO (1) WO2010087987A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USH2268H1 (en) * 2009-01-30 2012-04-03 The United States Of America, As Represented By The Secretary Of The Navy Microtiter plate to mitigate cell distribution bias from meniscus edge
DE102014107934A1 (en) 2014-06-05 2015-12-17 Carl Zeiss Microscopy Gmbh A process for the microscopic imaging of samples of trays of filled with fluid wells of a microtiter plate
JP5768174B1 (en) * 2014-06-24 2015-08-26 日本写真印刷株式会社 Culture vessel
DE102015111426B3 (en) 2015-07-14 2016-10-20 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. A method for phase contrast microscopy and phase contrast microscope

Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4599314A (en) * 1983-06-14 1986-07-08 Hsc Research Development Corporation Multiple vessel specimen tray with lid for releasably adhering vessel covers
US4599315A (en) 1983-09-13 1986-07-08 University Of California Regents Microdroplet test apparatus
US4829006A (en) 1988-02-01 1989-05-09 Difco Laboratories Centrifugation vial and cluster tray
US5002889A (en) 1988-10-21 1991-03-26 Genetic Systems Corporation Reaction well shape for a microwell tray
US5741463A (en) * 1993-04-19 1998-04-21 Sanadi; Ashok Ramesh Apparatus for preventing cross-contamination of multi-well test plates
US6074614A (en) 1995-06-07 2000-06-13 Molecular Devices Corporation Multi-assay plate cover for elimination of meniscus
US20010007642A1 (en) 1998-03-03 2001-07-12 Marc Feiglin Sealing apparatus for use with microplates
US20030032002A1 (en) * 2001-07-27 2003-02-13 Evelyn Wang Cell isolation and screening device and method of using same
US6581761B1 (en) 1999-11-02 2003-06-24 Bausch & Lomb Incorporated Mesh tray assembly
US20030124029A1 (en) * 2001-09-07 2003-07-03 Webb Brian L. Microcolumn-platform based array for high-throughput analysis
US20030235519A1 (en) 2002-06-24 2003-12-25 Corning Incorporated Protein crystallography hanging drop lid that individually covers each of the wells in a microplate
US20040009583A1 (en) 2002-02-05 2004-01-15 Genome Therapeutics Corporation Seal for microtiter plate and methods of use thereof
US6716350B2 (en) 2002-05-03 2004-04-06 Millipore Corporation Microplate protective tray undercover
US20060029524A1 (en) 2004-08-05 2006-02-09 3M Innovative Properties Company Sample processing device positioning apparatus and methods
USD562463S1 (en) 2004-11-03 2008-02-19 Hoffmann-La Roche Inc. Microtiter plate
US20100197004A1 (en) * 2009-01-30 2010-08-05 United States Government, As Represented By The Secretary Of The Navy Microtiter plate to mitigate cell distribution bias from meniscus edge

Patent Citations (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4599314A (en) * 1983-06-14 1986-07-08 Hsc Research Development Corporation Multiple vessel specimen tray with lid for releasably adhering vessel covers
US4599315A (en) 1983-09-13 1986-07-08 University Of California Regents Microdroplet test apparatus
US4829006A (en) 1988-02-01 1989-05-09 Difco Laboratories Centrifugation vial and cluster tray
US5002889A (en) 1988-10-21 1991-03-26 Genetic Systems Corporation Reaction well shape for a microwell tray
US5741463A (en) * 1993-04-19 1998-04-21 Sanadi; Ashok Ramesh Apparatus for preventing cross-contamination of multi-well test plates
US6074614A (en) 1995-06-07 2000-06-13 Molecular Devices Corporation Multi-assay plate cover for elimination of meniscus
US20010007642A1 (en) 1998-03-03 2001-07-12 Marc Feiglin Sealing apparatus for use with microplates
US6581761B1 (en) 1999-11-02 2003-06-24 Bausch & Lomb Incorporated Mesh tray assembly
US20030032002A1 (en) * 2001-07-27 2003-02-13 Evelyn Wang Cell isolation and screening device and method of using same
US7169577B2 (en) * 2001-07-27 2007-01-30 Surface Logix, Inc. Cell isolation and screening device and method of using same
US20030124029A1 (en) * 2001-09-07 2003-07-03 Webb Brian L. Microcolumn-platform based array for high-throughput analysis
US20040009583A1 (en) 2002-02-05 2004-01-15 Genome Therapeutics Corporation Seal for microtiter plate and methods of use thereof
US6716350B2 (en) 2002-05-03 2004-04-06 Millipore Corporation Microplate protective tray undercover
US20030235519A1 (en) 2002-06-24 2003-12-25 Corning Incorporated Protein crystallography hanging drop lid that individually covers each of the wells in a microplate
US20060029524A1 (en) 2004-08-05 2006-02-09 3M Innovative Properties Company Sample processing device positioning apparatus and methods
USD562463S1 (en) 2004-11-03 2008-02-19 Hoffmann-La Roche Inc. Microtiter plate
US20100197004A1 (en) * 2009-01-30 2010-08-05 United States Government, As Represented By The Secretary Of The Navy Microtiter plate to mitigate cell distribution bias from meniscus edge

Also Published As

Publication number Publication date Type
US20100197004A1 (en) 2010-08-05 application
WO2010087987A1 (en) 2010-08-05 application

Similar Documents

Publication Publication Date Title
US5419278A (en) Vapor equilibration tray for growing protein crystals
US5285907A (en) Modular tube rack arrays
US5026649A (en) Apparatus for growing tissue cultures in vitro
US20050084923A1 (en) Methods for cultivating and analyzing microbial individual cell cultures
US6485690B1 (en) Multiple fluid sample processor and system
US4349632A (en) Tissue culture cluster dish
US6083761A (en) Method and apparatus for transferring and combining reagents
EP0307048A2 (en) Cell culture flask utilizing a membrane barrier
US7527769B2 (en) Microtitre plate with a relieved perimeter
US20060013031A1 (en) Assay systems with adjustable fluid communication
US5417576A (en) Means and method for microbiological growth and in situ observation with microscopes
US20030104494A1 (en) Assay systems with adjustable fluid communication
US7332271B2 (en) Apparatus and methods for parallel processing of micro-volume liquid reactions
US5272083A (en) Culture device and method of use having a detachable cell or tissue growth surface
US20070172814A1 (en) Method of Testing the Safety and Efficacy of a Drug
US20070243523A1 (en) Methods and Apparatus for the Manipulation of Particle Suspensions and Testing Thereof
US5779984A (en) Pipette tip rack and refill pack containing large maximized volume freely nestable pipette tips
US20030111494A1 (en) Method and apparatus for high-throughput sample handling process line
US20050112030A1 (en) Meshwell plates
US5139951A (en) Culture device having a detachable cell or tissue growth surface
US4296205A (en) Cell culture and continuous dialysis flask and method
US20020189374A1 (en) Multiwell test apparatus
US5468638A (en) Cell culture insert
US7358098B2 (en) Device for capturing beads and method and apparatus for arraying beads
US20040195193A1 (en) Tube rack accommodating a range of tube diameters

Legal Events

Date Code Title Description
AS Assignment

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:GUTTING, BRADFORD W.;MACKIE, RYAN S.;ANDREWS, JR., GEORGE A.;SIGNING DATES FROM 20090123 TO 20090129;REEL/FRAME:022287/0736

Owner name: NAVY, UNITED STATES OF AMERICA, THE, REPRESENTED B