US5783401A - Immunoenzymatic detection device - Google Patents
Immunoenzymatic detection device Download PDFInfo
- Publication number
- US5783401A US5783401A US08/269,637 US26963794A US5783401A US 5783401 A US5783401 A US 5783401A US 26963794 A US26963794 A US 26963794A US 5783401 A US5783401 A US 5783401A
- Authority
- US
- United States
- Prior art keywords
- reservoir
- membrane
- liquid
- reagent
- median
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/97—Test strip or test slide
Definitions
- the present invention relates to a device for detecting the presence, in a biological medium, of chemical or biological substances, even present in very small concentrations, of the order of a nanogram per milliliter.
- the invention relates more particularly to rapid biological tests using the antigen-antibody reaction to reveal, by coloration of a substrate, the presence of substances which it is desired to detect.
- Diagnosis strips have been known for a long time for carrying out detection tests in urine. Strips are also known comprising a square of cellulose for determining the quantity of glucose from a drop of blood deposited on the cellulose square, held for a minute thereon, then washed so as to read the coloured reaction.
- Immuno-enzymatic detection devices also exist using antigens or antibodies, but requiring, on the one hand, staff for taking blood samples, and handling and, on the other hand, using a complex, expensive and especially very time consuming system (three hours because of the repeated washing following periods of incubation of half an hour between each phase).
- the device of the present invention overcomes these drawbacks and makes available for patients, doctors, small care centres, transfusion centres, emergency services, etc . . . a whole range of high speed tests, since this device, which only requires a drop of liquid to be analysed, makes diagnosis possible in the few following minutes without any human intervention, by simply reading the coloration of a substrate pellet situated in the lower part of the device, whence its reduced cost and great ease of use.
- the present invention provides a device for detecting the presence of substances in a liquid or solid biological medium, even when they are present in very small concentrations, of the order of a nanogram per milliliter, which device is characterized in that it comprises, in combination:
- a first filter membrane which is temporarily impermeable in the presence of a reaction sample; situated at the base of the reservoir and whose upper face carries a thin layer of a reagent (A), intended to react with the drop of biological medium to be analysed,
- a second transparent or opaque plastic material film cut out so as to form a median reservoir for receiving the liquid coming from the passage of the drop through the first said filter membrane, which median reservoir contains a reagent (B) capable of reacting with said liquid,
- a second membrane which is temporarily impermeable, also a filter, situated at the base of the median reservoir and through which the liquid passes after its contact with the reagent (B) in said median reservoir,
- a third transparent or opaque plastic material film cut out so as to form a lower reservoir which contains an appropriate carrier for a reagent (C) which, by contact with the liquid which reaches the lower reservoir after passing through the membrane, has a coloration adapted to indicate the presence or absence of the substance sought, in the biological medium analysed.
- C reagent
- the reagent (A) is an antibody or an antigen marked by an appropriate marker and preferably lyophilized.
- the reagent (B) is. formed by microspheres made from an appropriate material and coated with an antigen or an antibody; the reagent (C) is a substrate capable of reacting with the marker of reagent (A) so as to give, a coloration in contact with the reagent (A).
- the reagent (A) is formed by an unmarked antibody present in a predetermined amount and the device comprises, following the upper reservoir, a reagent (D) formed by marked antibodies contained in liposomes.
- the reagent (C) is carried by an appropriate carrier such more particularly as a cellulose pellet or the like.
- the device of the present invention comprises a plurality of reservoirs cut out from each film so as to permit the simultaneous detection of several different substances.
- the reagent (A) is formed by a plurality of different antibodies or antigens specific of a disease and the reagent (C) comprises a plurality of substrates capable of reacting with one or more of the markers which form the reagent (A) so as to give, one or more colorations.
- the filter membranes are made from gelatin and glass fibres.
- FIG. 1 is a longitudinal sectional view of the device of the invention
- FIG. 2 is a perspective view thereof
- FIG. 3 is a top view thereof
- FIG. 4 is a bottom view.
- the device shown in the drawing is formed of superimposed elements 1 to 5, all of the same length, e.g. 100 mm, and of the same width, e.g. 20 mm, all having at the same position one or more cut-outs (reservoirs R1, R2 and R3), which may be circular, with a diameter of 5 to 20 mm depending on the required diagnoses, but of different thicknesses, namely respectively from top to bottom:
- a transparent or opaque plastic material film 1, 0.5 to 5 mm thick, for receiving at R1 the drop of liquid to be analysed.
- a gelatin and glass fibre membrane 2 of a thickness from 0.5 to 1.5 mm, supporting on its upper face a thin reagent layer (A) for reacting immediately with the drop of liquid to be analysed.
- the latter in contact with the liquid coming from the filter membrane 4 has a different coloration depending on whether the substance sought is present or not in the liquid to be analysed.
- the reaction is then read by simply turning over the device after one to three minutes depending on the different analyses.
- the reagent (A) is formed by a lyophilized antibody or antigen, marked by any suitable marker, such as enzymes, fluorescence or radioactivity, etc . . .
- the membrane 2 is made from gelatin on glass fibres, with a porosity varying from 0.2 to 50 microns depending on the desired diagnoses, and supports the reagent (A) on its upper face.
- the membrane 4 is also made from gelatin on glass fibres, of a porosity between 0.2 and 50 microns.
- the reagent (B) is formed by microspheres of a diameter varying from 10 to 250 microns, made from iron or any other metal, metal alloys, polyacrylamide or any other material, coated with hydroxyapatite and protein A for solidly fixing an antigen or an antibody after agitation. They are then placed in contact for one to six hours with ethanolamine so as to block all the amine sites and thus avoid any other chemical reaction than the one concerning the antigen-antibody.
- the reagent (C) is a substrate of the marker of reagent (A); for example, in the case where the antigen or antibody is marked by an enzyme, if this enzyme is a peroxydase, the substrate will be 3--3' 5--5' tetramethylbenzidine for a blue coloration or oxyphenyldiamine for an orange coloration.
- several reservoirs may exist for detecting several different substances.
- the hepatitis diagnosis test to associate a rapid evaluation of the transaminases.
- evaluation of the cardiac enzyme rate gives a very precious indication on the prognosis and the seriousness of the lesions.
- the sero positivity of AIDS not being sufficient, it may be completed on the same strip by an immunological test capable of indicating the quality and number of lymphocytes T, and thus to permit the follow-up of sero positives.
- the reservoirs may be square, rectangular, oval, etc . . .
- the number of films may be greater than or less than the three described above.
- the device of the present invention makes it possible to track down numerous diseases involving an agent (the antigen) and the reaction of the organism to this agent, namely the revelation and detecting of the antibodies against this agent from a drop of any liquid coming from the human body (blood, serum, plasma, urine, saliva, tears, cerebraspinal fluid, ureteral and vaginal humours, mother's breast milk, liquids swabbed during an operation, or from organs to be grafted, for detecting AIDS for example) etc . . .
- Detection may also be made from fragments of any solids previously solubilized or simply agitated with a little water.
- the detection device of the present invention also applies to veterinary medicine for tracking down infections or detecting of hormones for example.
- the invention also applies to agriculture for microdetermination.
- the device of the present invention is capable of detecting the antigen rate of certain cancers, solving the dramatic problem of treated patients, in whom it is practically impossible to know sufficiently early if they will have a relapse, metastases, whereas the device of the present invention makes it possible to reveal them by the coloured reaction as soon as the antigen rate exceeds a certain limit.
- the detection device of the present invention operates as follows:
- the antigen is bonded to the antibody (A) at R1, passes through R2 without being retained by the spheres and arrives at R3 to colour the substrate.
- (A) will be a marked antigen and an antibody is fixed on the microspheres for retaining this marked antigen in the absence of antibodies in the serum.
- unmarked antibodies are fixed, fixing for example 10 ng/ml of antigen. If the serum contains more, it will be later fixed on marked antibodies enclosed in liposomes which open after a minute. Same principle for the semi-quantitative titration of antibodies.
- the device of the present invention is a liquid crystal display
- the test makes it possible to detect several different substances with the same drop of blood by simply associating, in the above described reservoirs, the reagents corresponding to said substances. If the test is positive, it is because at least one of the substances sought is present in the drop examined. Thus, for example, different cancer markers may be placed in the same test (digestive for example: ACE for the colon, C19-9 for the pancreas, etc . . . ).Since it is improbable that the subject has several different cancers, if the test is positive, it directs the doctor towards the digestive sphere and then to make separate tests for each cancer and each member so as to thus localize a beginning tumour.
- the surgeon or anaesthetist may use a test in which the microspheres of the reagent (B) are covered either with different antigens (HIV, HTLV, hepatitis, etc . . . ), or a test with a percentage of spheres marked with HIV, another portion with HTLV, etc . . . , so that a drop of blood, in a single test with the same device, may give indications of the highest importance.
- different antigens HIV, HTLV, hepatitis, etc . . .
- a test with a percentage of spheres marked with HIV, another portion with HTLV, etc . . . so that a drop of blood, in a single test with the same device, may give indications of the highest importance.
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
Description
Claims (20)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/269,637 US5783401A (en) | 1987-10-05 | 1994-07-01 | Immunoenzymatic detection device |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
FR8713879 | 1987-10-05 | ||
FR878713879A FR2621393B1 (en) | 1987-10-05 | 1987-10-05 | DEVICE FOR IMMUNOENZYMATIC DETECTION OF SUBSTANCES FROM A DROP OF BLOOD OR LIQUID FROM ANY BIOLOGICAL MEDIUM |
PCT/FR1988/000493 WO1989003039A1 (en) | 1987-10-05 | 1988-10-05 | Immunoenzymatic detection device |
US36832990A | 1990-01-17 | 1990-01-17 | |
US78216191A | 1991-10-24 | 1991-10-24 | |
US08/269,637 US5783401A (en) | 1987-10-05 | 1994-07-01 | Immunoenzymatic detection device |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US78216191A Continuation | 1987-10-05 | 1991-10-24 |
Publications (1)
Publication Number | Publication Date |
---|---|
US5783401A true US5783401A (en) | 1998-07-21 |
Family
ID=9355616
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US08/269,637 Expired - Fee Related US5783401A (en) | 1987-10-05 | 1994-07-01 | Immunoenzymatic detection device |
Country Status (7)
Country | Link |
---|---|
US (1) | US5783401A (en) |
EP (1) | EP0334947B1 (en) |
JP (1) | JP2676108B2 (en) |
AT (1) | ATE86740T1 (en) |
DE (1) | DE3879180T2 (en) |
FR (1) | FR2621393B1 (en) |
WO (1) | WO1989003039A1 (en) |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6352862B1 (en) | 1989-02-17 | 2002-03-05 | Unilever Patent Holdings B.V. | Analytical test device for imuno assays and methods of using same |
US6859570B2 (en) * | 1997-03-14 | 2005-02-22 | Trustees Of Tufts College, Tufts University | Target analyte sensors utilizing microspheres |
US20050047972A1 (en) * | 2003-08-28 | 2005-03-03 | Imants Lauks | Lateral flow diagnostic devices with instrument controlled fluidics |
US20050130188A1 (en) * | 1997-03-14 | 2005-06-16 | The Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
US20080145835A1 (en) * | 2005-01-31 | 2008-06-19 | Realbio Technologies Ltd | Multistep Reaction Lateral Flow Capillary Device |
US20090143244A1 (en) * | 1997-05-23 | 2009-06-04 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
US20090203059A1 (en) * | 2006-04-07 | 2009-08-13 | Mologic Ltd. | Protease detection product |
USRE43097E1 (en) | 1994-10-13 | 2012-01-10 | Illumina, Inc. | Massively parallel signature sequencing by ligation of encoded adaptors |
US9399795B2 (en) | 1998-06-24 | 2016-07-26 | Illumina, Inc. | Multiplex decoding of array sensors with microspheres |
US9404911B2 (en) | 2008-04-21 | 2016-08-02 | Quidel Corporation | Integrated assay device and housing |
US9726581B2 (en) | 2011-12-22 | 2017-08-08 | Realbio Technologies Ltd. | Sequential lateral flow capillary device for analyte determination |
US9952211B2 (en) | 2008-06-29 | 2018-04-24 | Realbio Technologies Ltd. | Liquid-transfer device particularly useful as a capturing device in a biological assay process |
US10107804B2 (en) | 2001-03-23 | 2018-10-23 | Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2666898A1 (en) * | 1990-09-18 | 1992-03-20 | Toledano Jacques | Device and method for the rapid assay of membrane receptors or their ligands |
DE4205894A1 (en) * | 1992-02-26 | 1993-09-02 | Martin Rahe | DEVICE FOR DETECTING AND / OR MEASURING OR CONTROLLING THE TEXTURE, ESPECIALLY CHEMICAL AND / OR BIOLOGICAL RELATIONSHIPS WITH THE HELP OF AT LEAST ONE INDICATOR IN LIQUID MILIEU, ESPECIALLY AQUEOUS MILIEU, AND LIKE |
FR2703788B1 (en) * | 1993-04-09 | 1996-12-20 | Jacques Toledano | ANTI-IDIOTYPE DEVICE AND METHOD FOR IMMUNOLOGICAL DETECTION. |
FR2872288B1 (en) * | 2004-06-28 | 2007-08-03 | Biomerieux Sa | INTEGRATED ANALYSIS DEVICE ADAPTABLE ON A CONTAINER OF A SAMPLE TO BE ANALYZED |
GB2435511A (en) | 2006-02-23 | 2007-08-29 | Mologic Ltd | Protease detection |
GB2435512A (en) | 2006-02-23 | 2007-08-29 | Mologic Ltd | A binding assay and assay device |
GB2435510A (en) | 2006-02-23 | 2007-08-29 | Mologic Ltd | Enzyme detection product and methods |
Citations (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4138394A (en) * | 1976-01-24 | 1979-02-06 | Ajinomoto Co., Inc. | Peptide derivatives and a method of measuring collagenase activity |
WO1980002077A1 (en) * | 1979-03-26 | 1980-10-02 | Gen Electric | Method and apparatus for measuring antibody levels |
US4258001A (en) * | 1978-12-27 | 1981-03-24 | Eastman Kodak Company | Element, structure and method for the analysis or transport of liquids |
US4366241A (en) * | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
US4446232A (en) * | 1981-10-13 | 1984-05-01 | Liotta Lance A | Enzyme immunoassay with two-zoned device having bound antigens |
US4459358A (en) * | 1982-12-29 | 1984-07-10 | Polaroid Corporation | Multilayer element for analysis |
EP0133700A2 (en) * | 1983-08-10 | 1985-03-06 | E.I. Du Pont De Nemours And Company | Improved multilayered test device for detecting analytes in liquid test samples |
US4587102A (en) * | 1981-06-05 | 1986-05-06 | Fuji Photo Film Co., Ltd. | Multi-layer analysis element utilizing specific binding reaction |
WO1986004683A1 (en) * | 1985-02-08 | 1986-08-14 | Boehringer Biochemia Robin S.P.A. | Determination of clinical parameters by enzyme immunoprocess |
EP0191640A2 (en) * | 1985-02-14 | 1986-08-20 | Abbott Laboratories | Concentrating immunochemical test strip |
US4608231A (en) * | 1984-12-12 | 1986-08-26 | Becton, Dickinson And Company | Self-contained reagent package device for an assay |
EP0200540A2 (en) * | 1985-05-01 | 1986-11-05 | EASTMAN KODAK COMPANY (a New Jersey corporation) | Analytical element and method for determination of creatine kinase isoenzyme |
EP0231830A2 (en) * | 1986-01-23 | 1987-08-12 | Wako Pure Chemical Industries, Ltd. | Method of immunochemical assay |
EP0236768A1 (en) * | 1986-02-20 | 1987-09-16 | Fuji Photo Film Co., Ltd. | Multi-layered element for quantitative analysis of immuno-reactant |
US4752572A (en) * | 1985-08-30 | 1988-06-21 | Eastman Kodak Company | Lipid vesicles containing labeled species and their analytical uses |
US4774174A (en) * | 1981-01-23 | 1988-09-27 | Baxter Travenol Laboratories, Inc. | Solid phase system for ligand assay |
US4803154A (en) * | 1985-04-25 | 1989-02-07 | Shionogi & Co., Ltd. | Multi-spot enzyme immunoassay method |
US4923680A (en) * | 1987-09-18 | 1990-05-08 | Eastman Kodak Company | Test device containing an immunoassay filter with a flow-delaying polymer |
US5079141A (en) * | 1985-05-14 | 1992-01-07 | Orion Corporation Ltd. | Apparatus and method for performing chemical analyses and immunoassays |
US5217905A (en) * | 1988-04-28 | 1993-06-08 | Compagnie Oris Industrie S.A. | Device and method for the rapid qualitative and quantitative determination of the presence of a reactive ligand in a fluid |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3048799A1 (en) * | 1980-12-23 | 1982-07-08 | Boehringer Mannheim Gmbh, 6800 Mannheim | REAGENT STRIP FOR ANALYTICAL PURPOSES |
-
1987
- 1987-10-05 FR FR878713879A patent/FR2621393B1/en not_active Expired - Lifetime
-
1988
- 1988-10-05 EP EP88909553A patent/EP0334947B1/en not_active Expired - Lifetime
- 1988-10-05 AT AT88909553T patent/ATE86740T1/en not_active IP Right Cessation
- 1988-10-05 WO PCT/FR1988/000493 patent/WO1989003039A1/en active IP Right Grant
- 1988-10-05 DE DE88909553T patent/DE3879180T2/en not_active Expired - Fee Related
- 1988-10-05 JP JP63508808A patent/JP2676108B2/en not_active Expired - Lifetime
-
1994
- 1994-07-01 US US08/269,637 patent/US5783401A/en not_active Expired - Fee Related
Patent Citations (22)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4138394A (en) * | 1976-01-24 | 1979-02-06 | Ajinomoto Co., Inc. | Peptide derivatives and a method of measuring collagenase activity |
US4258001A (en) * | 1978-12-27 | 1981-03-24 | Eastman Kodak Company | Element, structure and method for the analysis or transport of liquids |
WO1980002077A1 (en) * | 1979-03-26 | 1980-10-02 | Gen Electric | Method and apparatus for measuring antibody levels |
US4366241A (en) * | 1980-08-07 | 1982-12-28 | Syva Company | Concentrating zone method in heterogeneous immunoassays |
US4366241B1 (en) * | 1980-08-07 | 1988-10-18 | ||
US4774174A (en) * | 1981-01-23 | 1988-09-27 | Baxter Travenol Laboratories, Inc. | Solid phase system for ligand assay |
US4587102A (en) * | 1981-06-05 | 1986-05-06 | Fuji Photo Film Co., Ltd. | Multi-layer analysis element utilizing specific binding reaction |
US4446232A (en) * | 1981-10-13 | 1984-05-01 | Liotta Lance A | Enzyme immunoassay with two-zoned device having bound antigens |
US4459358A (en) * | 1982-12-29 | 1984-07-10 | Polaroid Corporation | Multilayer element for analysis |
EP0133700A2 (en) * | 1983-08-10 | 1985-03-06 | E.I. Du Pont De Nemours And Company | Improved multilayered test device for detecting analytes in liquid test samples |
US4608231A (en) * | 1984-12-12 | 1986-08-26 | Becton, Dickinson And Company | Self-contained reagent package device for an assay |
WO1986004683A1 (en) * | 1985-02-08 | 1986-08-14 | Boehringer Biochemia Robin S.P.A. | Determination of clinical parameters by enzyme immunoprocess |
EP0191640A2 (en) * | 1985-02-14 | 1986-08-20 | Abbott Laboratories | Concentrating immunochemical test strip |
US4803154A (en) * | 1985-04-25 | 1989-02-07 | Shionogi & Co., Ltd. | Multi-spot enzyme immunoassay method |
EP0200540A2 (en) * | 1985-05-01 | 1986-11-05 | EASTMAN KODAK COMPANY (a New Jersey corporation) | Analytical element and method for determination of creatine kinase isoenzyme |
US5079141A (en) * | 1985-05-14 | 1992-01-07 | Orion Corporation Ltd. | Apparatus and method for performing chemical analyses and immunoassays |
EP0212989B1 (en) * | 1985-08-30 | 1992-02-19 | EASTMAN KODAK COMPANY (a New Jersey corporation) | Lipid vesicles containing labeled species and their analytical uses |
US4752572A (en) * | 1985-08-30 | 1988-06-21 | Eastman Kodak Company | Lipid vesicles containing labeled species and their analytical uses |
EP0231830A2 (en) * | 1986-01-23 | 1987-08-12 | Wako Pure Chemical Industries, Ltd. | Method of immunochemical assay |
EP0236768A1 (en) * | 1986-02-20 | 1987-09-16 | Fuji Photo Film Co., Ltd. | Multi-layered element for quantitative analysis of immuno-reactant |
US4923680A (en) * | 1987-09-18 | 1990-05-08 | Eastman Kodak Company | Test device containing an immunoassay filter with a flow-delaying polymer |
US5217905A (en) * | 1988-04-28 | 1993-06-08 | Compagnie Oris Industrie S.A. | Device and method for the rapid qualitative and quantitative determination of the presence of a reactive ligand in a fluid |
Cited By (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6352862B1 (en) | 1989-02-17 | 2002-03-05 | Unilever Patent Holdings B.V. | Analytical test device for imuno assays and methods of using same |
USRE43097E1 (en) | 1994-10-13 | 2012-01-10 | Illumina, Inc. | Massively parallel signature sequencing by ligation of encoded adaptors |
US20090156425A1 (en) * | 1997-03-14 | 2009-06-18 | Walt David R | Methods for detecting target analytes and enzymatic reactions |
US20050130188A1 (en) * | 1997-03-14 | 2005-06-16 | The Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
US7622294B2 (en) | 1997-03-14 | 2009-11-24 | Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
US9377388B2 (en) | 1997-03-14 | 2016-06-28 | Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
US10241026B2 (en) | 1997-03-14 | 2019-03-26 | Trustees Of Tufts College | Target analyte sensors utilizing microspheres |
US6859570B2 (en) * | 1997-03-14 | 2005-02-22 | Trustees Of Tufts College, Tufts University | Target analyte sensors utilizing microspheres |
US20090143244A1 (en) * | 1997-05-23 | 2009-06-04 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
US8361713B2 (en) | 1997-05-23 | 2013-01-29 | Illumina, Inc. | System and apparatus for sequential processing of analytes |
US9273354B2 (en) | 1997-05-23 | 2016-03-01 | Illumina, Inc. | System and apparatus for sequential processing of analytes |
US9399795B2 (en) | 1998-06-24 | 2016-07-26 | Illumina, Inc. | Multiplex decoding of array sensors with microspheres |
US10107804B2 (en) | 2001-03-23 | 2018-10-23 | Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
US8124026B2 (en) | 2003-08-28 | 2012-02-28 | Epocal Inc. | Lateral flow diagnostic devices with instrument controlled fluidics |
US20100202926A1 (en) * | 2003-08-28 | 2010-08-12 | Epocal Inc. | Lateral flow diagnostic devices with instrument controlled fluidics |
US7722817B2 (en) | 2003-08-28 | 2010-05-25 | Epocal Inc. | Lateral flow diagnostic devices with instrument controlled fluidics |
US20050047972A1 (en) * | 2003-08-28 | 2005-03-03 | Imants Lauks | Lateral flow diagnostic devices with instrument controlled fluidics |
US9101927B2 (en) * | 2005-01-31 | 2015-08-11 | Realbio Technologies Ltd. | Multistep reaction lateral flow capillary device |
US20080145835A1 (en) * | 2005-01-31 | 2008-06-19 | Realbio Technologies Ltd | Multistep Reaction Lateral Flow Capillary Device |
US10335783B2 (en) | 2005-01-31 | 2019-07-02 | Realbio Technologies, Ltd. | Multistep reaction lateral flow capillary device |
US20090203059A1 (en) * | 2006-04-07 | 2009-08-13 | Mologic Ltd. | Protease detection product |
US9404911B2 (en) | 2008-04-21 | 2016-08-02 | Quidel Corporation | Integrated assay device and housing |
US9952211B2 (en) | 2008-06-29 | 2018-04-24 | Realbio Technologies Ltd. | Liquid-transfer device particularly useful as a capturing device in a biological assay process |
US11280785B2 (en) | 2008-06-29 | 2022-03-22 | Realbio Technologies Ltd. | Liquid-transfer device particularly useful as a capturing device in a biological assay process |
US9726581B2 (en) | 2011-12-22 | 2017-08-08 | Realbio Technologies Ltd. | Sequential lateral flow capillary device for analyte determination |
US10598572B2 (en) | 2011-12-22 | 2020-03-24 | Realbio Technologies, Ltd. | Sequential lateral capillary flow device for analyte determination |
Also Published As
Publication number | Publication date |
---|---|
ATE86740T1 (en) | 1993-03-15 |
FR2621393A1 (en) | 1989-04-07 |
DE3879180D1 (en) | 1993-04-15 |
JPH02501954A (en) | 1990-06-28 |
WO1989003039A1 (en) | 1989-04-06 |
JP2676108B2 (en) | 1997-11-12 |
EP0334947A1 (en) | 1989-10-04 |
FR2621393B1 (en) | 1991-12-13 |
DE3879180T2 (en) | 1993-10-07 |
EP0334947B1 (en) | 1993-03-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US5783401A (en) | Immunoenzymatic detection device | |
US6316205B1 (en) | Assay devices and methods of analyte detection | |
EP0389003B1 (en) | Solid-phase analytical device and method for using same | |
US4956302A (en) | Lateral flow chromatographic binding assay device | |
US6214629B1 (en) | Analytical test device and method for use in medical diagnoses | |
JP4469082B2 (en) | Apparatus and method for analyte detection | |
JP4104669B2 (en) | Removable element tester | |
US6410341B1 (en) | Analytical test device and method for use in medical diagnoses | |
US6673628B2 (en) | Analytical test device and method | |
EP0306772B1 (en) | Lateral flow chromatographic binding assay device | |
US4826759A (en) | Field assay for ligands | |
JP3021380B2 (en) | One-step assay method and apparatus for whole blood | |
EP0269876B1 (en) | Sample focuser for solid-phase analytical device | |
CN104042253B (en) | Rotatable dish type fluid sample collection device | |
JP4233686B2 (en) | Immunochromatography equipment housing | |
US8202487B2 (en) | Multiple analyte assay devices | |
ZA200107447B (en) | Sample collection and testing system. | |
CN1256754A (en) | Analytical device for membrane-based assays | |
US5238847A (en) | Test kit and process for the determination of an analyte in a pasty sample | |
US7947490B2 (en) | Recording assay device | |
US5827681A (en) | Rapid detection and drug sensitivity of malaria | |
CN102135535A (en) | Immune colloidal metal detection technology capable of directly performing semi-quantitative analysis, preparation method and application | |
US8623291B2 (en) | Multiple analyte assay device | |
EP2270508A1 (en) | Membrane assay method and kit | |
WO2014168580A1 (en) | Double-chamber bi-directional reverse flow device |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FPAY | Fee payment |
Year of fee payment: 4 |
|
REFU | Refund |
Free format text: REFUND - PAYMENT OF MAINTENANCE FEE, 8TH YEAR, LARGE ENTITY (ORIGINAL EVENT CODE: R1552); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY |
|
FEPP | Fee payment procedure |
Free format text: PAT HOLDER NO LONGER CLAIMS SMALL ENTITY STATUS, ENTITY STATUS SET TO UNDISCOUNTED (ORIGINAL EVENT CODE: STOL); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY |
|
REFU | Refund |
Free format text: REFUND - PAYMENT OF MAINTENANCE FEE, 8TH YR, SMALL ENTITY (ORIGINAL EVENT CODE: R2552); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY Free format text: REFUND - 7.5 YR SURCHARGE - LATE PMT W/IN 6 MO, SMALL ENTITY (ORIGINAL EVENT CODE: R2555); ENTITY STATUS OF PATENT OWNER: LARGE ENTITY |
|
REMI | Maintenance fee reminder mailed | ||
FPAY | Fee payment |
Year of fee payment: 8 |
|
SULP | Surcharge for late payment |
Year of fee payment: 7 |
|
AS | Assignment |
Owner name: BANK OF AMERICA, N.A., AS AGENT, CALIFORNIA Free format text: GRANT OF PATENT SECURITY INTEREST;ASSIGNOR:QUIDEL CORPORATION;REEL/FRAME:021658/0855 Effective date: 20081008 Owner name: BANK OF AMERICA, N.A., AS AGENT,CALIFORNIA Free format text: GRANT OF PATENT SECURITY INTEREST;ASSIGNOR:QUIDEL CORPORATION;REEL/FRAME:021658/0855 Effective date: 20081008 |
|
REMI | Maintenance fee reminder mailed | ||
LAPS | Lapse for failure to pay maintenance fees | ||
STCH | Information on status: patent discontinuation |
Free format text: PATENT EXPIRED DUE TO NONPAYMENT OF MAINTENANCE FEES UNDER 37 CFR 1.362 |
|
FP | Lapsed due to failure to pay maintenance fee |
Effective date: 20100721 |
|
AS | Assignment |
Owner name: QUIDEL CORPORATION, CALIFORNIA Free format text: NOTICE OF RELEASE OF GRANT OF PATENT SECURITY INTEREST;ASSIGNOR:BANK OF AMERICA, N.A., AS AGENT;REEL/FRAME:040789/0891 Effective date: 20161201 |