US4572910A - Triazolo[1,5-c]pyrimidines substituted by nitrogen-containing heterocyclic rings - Google Patents

Triazolo[1,5-c]pyrimidines substituted by nitrogen-containing heterocyclic rings Download PDF

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US4572910A
US4572910A US06/471,836 US47183683A US4572910A US 4572910 A US4572910 A US 4572910A US 47183683 A US47183683 A US 47183683A US 4572910 A US4572910 A US 4572910A
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triazolo
pyrimidine
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James J. Wade
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Riker Laboratories Inc
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Assigned to RIKER LABORATORIES, INC., 225-1N-07 3M CENTER, ST. PAUL, MN., A CORP. OF DEL. reassignment RIKER LABORATORIES, INC., 225-1N-07 3M CENTER, ST. PAUL, MN., A CORP. OF DEL. ASSIGNMENT OF ASSIGNORS INTEREST. Assignors: WADE, JAMES J.
Priority to US06/471,836 priority Critical patent/US4572910A/en
Priority to DK135184A priority patent/DK135184A/en
Priority to AU25238/84A priority patent/AU565545B2/en
Priority to NZ207349A priority patent/NZ207349A/en
Priority to AT84301383T priority patent/ATE38670T1/en
Priority to ZA841603A priority patent/ZA841603B/en
Priority to DE8484301383T priority patent/DE3475193D1/en
Priority to EP84301383A priority patent/EP0121341B1/en
Priority to IE516/84A priority patent/IE57026B1/en
Priority to JP59040265A priority patent/JPS59167592A/en
Priority to CA000448748A priority patent/CA1231335A/en
Priority to KR1019840001076A priority patent/KR900005836B1/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
    • C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
    • C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
    • C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
    • C07D239/32—One oxygen, sulfur or nitrogen atom
    • C07D239/42—One nitrogen atom
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
    • C07D239/02—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings
    • C07D239/24—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members
    • C07D239/28—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings not condensed with other rings having three or more double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to ring carbon atoms
    • C07D239/46—Two or more oxygen, sulphur or nitrogen atoms
    • C07D239/48—Two nitrogen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems

Definitions

  • the present invention relates to triazolo[1,5-c]pyrimidines, and more specifically to 1,2,4-triazolo[1,5-c]pyrimidines.
  • the pharmacological use of the compounds of the invention as bronchodilators, pharmaceutical compositions comprising the compounds, and processes for the preparation of the compounds are also within the scope of the invention.
  • 1,2,4-triazolo[1,5-c]pyrimidines are known to the art. Certain 1,2,4-triazolo[1,5-c]pyrimidines are disclosed as being bronchodilators in the patents discussed below, the compounds being referred to therein as triazolo[2,3-c]pyrimidines:
  • United Kingdom Patent No. 859,287 discloses 2-amino-1,2,4-triazolo[1,5-c]pyrimidines which are substituted on the pyrimidine ring at the 5, 7 and 8 positions by certain combinations of substituents selected from hydrogen, alkyl, halogen-substituted alkyl, hydroxy-substituted alkyl, alkoxy-substituted alkyl, alkenyl, cycloalkyl, amino, alkylamino, dialkylamino, phenyl, alkylthio, alkoxy and halogen substituents.
  • United Kingdom Patent No. 898,407 discloses processes for preparing certain of these compounds by subjecting the corresponding 1,2,4-triazolo[4,3-c]pyrimidines to an acid treatment, to an alkaline treatment, or to a heat treatment.
  • United Kingdom Patent No. 873,223 discloses 2-amino or 2-acetamido-1,2,4-triazolo[1,5-c]pyrimidines which are substituted on the pyrimidine ring at the 5, 7 and 8 positions by certain combinations of substituents selected from hydrogen, alkyl, halogen-substituted alkyl, alkoxy-substituted alkyl, alkenyl, cycloalkyl, alkylthio and halogen substituents.
  • United Kingdom Patent No. 897,870 discloses 2-alkylamino-1,2,4-triazolo[1,5-c]pyrimidines, 2-dialkylamino-1,2,4-triazolo[1,5-c]pyrimidines, and 1,2,4-triazolo[1,5-c]pyrimidines containing a piperidino or morpholino substituent bonded at the 2-position through the nitrogen atom, which compounds are substituted on the pyrimidine ring at the 5, 7 and 8 positions by certain combinations of substituents selected from hydrogen, alkyl, halogen-substituted alkyl, hydroxy-substituted alkyl, alkenyl and halogen substituents.
  • U.S. Pat. No. 4,269,980 discloses 5-, 7- and 8-(optionally substituted phenyl)-1,2,4-triazolo[1,5-c]pyrimidines. These compounds may be substituted at the 2-position by hydrogen or an alkyl substituent and are anxiolytic agents.
  • the present invention relates to 1,2,4-triazolo[1,5-c]pyrimidines which are bronchodilators.
  • the invention also relates to a method for inducing bronchodilation in a mammal using a 1,2,4-triazolo[1,5,-c]pyrimidine of the invention, and to pharmaceutical compositions comprising an effective amount of a 1,2,4-triazolo[1,5-c]pyrimidine of the invention and a pharmaceutically acceptable carrier.
  • the invention also relates to synthetic processes useful for preparing the 1,2,4-triazolo[1,5-c]pyrimidines of the invention.
  • the present invention relates to compounds of the formula I ##STR1## wherein R 2 is hydrogen or lower alkyl; at least one of R 5 and R 7 is ##STR2## wherein each X is independently oxygen, sulfur, sulfinyl, sulfonyl, methylene (--CH 2 --), imido (--NH--) or N-lower alkylimido ##STR3## and one of R 5 and R 7 may also be hydrogen, lower alkyl or phenyl; and R 8 is hydrogen, lower alkyl or phenyl; and pharmaceutically acceptable salts thereof.
  • Lower alkyl as used in the instant specification and claims designates straight or branched-chain alkyl groups containing one to about four carbon atoms. Preferred lower alkyl substituents are methyl and ethyl.
  • the presently preferred compounds of the invention are those wherein X is sulfur or oxygen and R 8 is hydrogen. These compounds are preferred because of their generally higher potency in protecting against histamineinduced contraction of isolated guinea pig tracheal tissue. This assay is discussed in greater detail below.
  • the bronchodilator activity of the compounds of Formula I was assessed by the measurement of effects on isolated tracheal spirals. This is a well-known and long established in vitro test method.
  • the bronchodilator activity was determined according to the following procedure: Female guinea pigs were sacrificed and each trachea removed and cut into a spiral strip. This strip was mounted in a constant temperature (37° C.) muscle bath having a volume of approximately 15 ml. The bathing medium was Krebs-Henseleit solution. Movement of the tracheal strip was measured by means of an isometric transducer connected to an electric recorder. The bath was aerated with a mixture of 95% carbon dioxide and 5% oxygen.
  • Contractions were induced in the strips by the addition of a suitable amount of histamine, acetylcholine or barium chloride.
  • the amount of a given compound of Formula I (measured in ug/ml) required to provide greater than 75% relaxation of drug-induced contraction is considered an effective concentration.
  • a well known standard bronchodilator, aminophylline requires concentrations of 50 ug/ml versus histamine, 100 ug/ml versus acetylcholine and 10 ug/ml versus barium chloride to provide greater than 75% relaxation.
  • Some of the compounds of Formula I were also found to have activity as mucolytics in an in vitro test for mucus production in which rats are orally dosed with compound prior to sacrifice. The trachea is then isolated and incubated with radiolabelled glucosamine and the effect of compounds on the incorporation of glucosamine into extracellular mucus is determined. An active compound reduces incorporation of glucosamine. Specific examples of preferred compounds which are active in this assay are:
  • the compounds of Formula I may be administered to mammals in order to obtain bronchodilation.
  • the compounds may be administered orally, parenterally or by inhalation. Preferably they are administered orally in tablets or capsules.
  • the usual effective human dose will be in the range of 0.1 to 50 mg/kg of body weight.
  • Salts of compounds of Formula I are generally prepared by reaction with an equimolar amount of a relatively strong acid, preferably an inorganic acid such as hydrochloric, sulfuric or phosphoric acid, in a polar solvent. Isolation of the salt is facilitated by the addition of a solvent in which the salt is insoluble, an example of such a solvent is diethyl ether.
  • a relatively strong acid preferably an inorganic acid such as hydrochloric, sulfuric or phosphoric acid
  • the compounds of Formula I can be combined with conventional pharmaceutical diluents and carriers to form such dosage forms as tablets, capsules, suspensions, solutions, suppositories and the like.
  • the pharmaceutical carrier employed may be, for example, either a solid or liquid.
  • solid carriers are lactose, terra alba, sucrose, talc, gelatin, agar, pectin, acacia, magnesium stearate, stearic acid, and the like.
  • Liquid carriers include syrup, peanut oil, olive oil, water and the like.
  • the carrier or diluent can include a time delay material well known to the art, such as glyceryl monostearate or glyceryl distearate, these being employed alone or, for example, in combination with a wax.
  • the compounds of Formula I may be prepared by several synthetic routes.
  • One such route is that shown in Scheme I below. This route is useful in preparing compounds wherein R 5 is hydrogen, lower alkyl or phenyl; R 7 is ##STR4## and R 2 , R 8 and X are as defined previously.
  • Each "alk” appearing in Scheme I is independently lower alkyl. ##STR5##
  • steps (1), (2) and (3) have previously been reported for the preparation of compounds wherein R 5 is hydrogen, methyl or ethyl and R 8 is hydrogen or methyl.
  • R 5 is hydrogen, methyl or ethyl
  • R 8 is hydrogen or methyl.
  • steps (1) and (2) were carried out as described in H. R. Henze et al., J. Org. Chem., 1952, 17, 1320 and H. R. Henze et al., J. Org. Chem., 1953, 18, 653, and step (3) was carried out as described in J. Chesterfield et al., J. Chem. Soc., 1955, 3478.
  • Example 1 hereinbelow details these steps.
  • Step (4) is carried out by reacting the optionally substituted 4-chloro-6-hydrazinopyrimidine of Formula VI with a heterocyclic amine of the formula VIA.
  • the reactants are heated together without solvent or optionally (and preferably) in any solvent which does not participate in the reaction such as water.
  • Two equivalents of the heterocyclic amine are preferably used.
  • one equivalent of the heterocyclic amine may be replaced by an inorganic base to neutralize the hydrogen chloride, but lower yields are obtained.
  • the reaction mixture is heated at a temperature up to or at its reflux temperature. A temperature is chosen which provides an adequate reaction rate. When water is used as the solvent, the temperature is generally in the range of 80° to 110° C.
  • Step (5) is carried out by reacting optionally substituted 4,6-dichloropyrimidines of Formula V with heterocyclic amines of the formula VIA.
  • This reaction is carried out by heating the reactants without solvent, or preferably in any solvent which does not participate in the reaction.
  • Two equivalents of the heterocyclic amine are preferably used, one to react with the chloropyrimidine and the other to neutralize the hydrogen chloride by-product.
  • an inorganic base may be used to neutralize the hydrogen chloride by-product, but lower yields of the desired product are generally obtained.
  • Heating is at a temperature up to and including the reflux temperature of the mixture. A temperature is chosen which provides an adequate reaction rate. If water is used as a solvent, the mixture is generally heated at its reflux temperature. Good yields of the desired product are isolated by conventional methods such as filtration, extraction or chromatography.
  • the novel intermediates of Formula VII are solids. Structural assignments are confirmed by infrared and nuclear magnetic resonance spectral analyses.
  • Step (6) is carried out by reacting the novel substituted 4-chloro-6-heterocyclicaminopyrimidine of Formula VII with hydrazine hydrate.
  • the reaction is facile and is generally carried out at moderate temperatures, for example, from -20° C. to the reflux temperature of the solvent.
  • the reaction is generally carried out by adding two equivalents of hydrazine hydrate to a solution of the pyrimidine.
  • the solvent will generally be a lower alkanol.
  • the product is separated by conventional methods such as filtration, extraction or chromatography and is the same novel intermediate of Formula VIII obtained from step (4).
  • Step (7) is carried out by reacting the intermediate of Formula VIII with an orthoester of formula VIIA.
  • orthoesters are well known and readily available.
  • suitable orthoesters include trimethyl orthoformate, triethyl orthoformate, triethyl orthoacetate, triethyl orthopropionate and the like. Since the orthoesters are liquids, it is convenient to mix the intermediates of Formula VIII with an excess of orthoester and to heat the mixture at reflux until reaction is complete. Good yields of the desired solid intermediates of Formula IX which are novel substituted 1,2,4-triazolo[4,3-c]pyrimidines are obtained by conventional methods.
  • step (8) the 1,2,4-triazolo[4,3-c]pyrimidines of Formula IX are heated with an aqueous acid and thereby converted to the desired compounds of the invention of Formula I wherein R 5 is ##STR6##
  • the preferred aqueous acids are carboxylic acids such as formic acid, acetic acid and propionic acid.
  • the reaction mixture is generally heated at reflux for up to several days.
  • the desired product is isolated by conventional methods. The structural assignments are made based on infrared and nuclear magnetic resonance spectral analyses. The products are generally white crytalline solids.
  • step (8) may be accomplished by continued heating of the reactants of step (7). This conversion occurs most readily when R 5 is hydrogen, and is carried out by using dimethyl sulfoxide as the solvent for the combined steps (7) and (8) as described in Example 104.
  • Synthetic Scheme II shows a method for the preparation of compounds of Formula I wherein both R 5 and R 7 are ##STR7## wherein X is as defined previously and may be the same or different in the two heterocyclic amino groups; and R 2 and R 8 are as defined previously. Alk is as defined previously. PG,13 ##STR8##
  • Step (1) of Scheme II requires reaction of the 4-chloro-6-hydrazino-2-methylthiopyrimidine of Formula X with a heterocyclic amine of the Formula VIA.
  • the 4-chloro-6-hydrazino-2-methylthiopyrimidines of Formula X are known compounds or may be prepared by conventional methods.
  • the reaction is generally conducted in an inert solvent, preferably water, optionally in the presence of an acid acceptor such as a tertiary organic amine, for example, triethylamine.
  • the reaction is best carried out using two equivalents of the amine of Formula VIA, one to react with the 4-chloro-6-hydrazino-2-methylthiopyrimidine and one to react with the hydrochloric acid which results.
  • the mixture is heated at reflux for several hours, then cooled.
  • the novel intermediate of Formula XI is obtained directly as a solid precipitate. Alternatively, it is obtained by extraction or chromatographic techniques.
  • Step (2) of Scheme II is carried out by mixing the intermediate of Formula XI with an orthoester of Formula VIIIA.
  • the reaction is carried out as described for step (7) of Scheme I.
  • the product obtained is a novel intermediate of Formula XII.
  • the product of this step generally is a mixture which is separated by chromatography, preferably high pressure liquid chromatography, to provide the crystalline solid. Occasionally the desired isomer is obtained in such high purity that chromatographic separation is unnecessary.
  • Step (3) of Scheme II requires heating of the intermediate of Formula XII in an excess of the heterocyclic amine of the Formula VIA, optionally in an inert solvent such as diglyme or dioxane.
  • the reaction is generally carried out at the reflux temperature of the mixture.
  • the product is isolated by conventional methods such as filtration, extraction or chromatography.
  • the compounds of Formula XIV are generally known or may be prepared by conventional methods. Known methods may be employed to vary substituents R 2 and R 7 .
  • the reaction of Scheme III occurs readily at moderate temperatures, for example from 0° C. up to the reflux temperature of the solvent. It is carried out either by adding the heterocyclic amine of Formula VIA to a solution of the intermediate of Formula XIV or vice versa.
  • the solvent may be inert solvent, for example water or dioxane.
  • the product of Formula I prepared in Scheme III is obtained in good yields by conventional isolation methods.
  • Synthetic Scheme IV illustrated below is a method for preparation of compounds of Formula I wherein R 2 and R 7 are independently hydrogen or lower alkyl; R 5 is ##STR11## wherein X is as defined previously; and R 8 is as defined previously. ##STR12##
  • reaction of Scheme IV is similar to reaction of step (3) of Scheme II.
  • the intermediates of Formula XV are known or may be prepared from known starting materials using known methods.
  • the products of Scheme IV are prepared and isolated as described for that step.
  • Hydrogen chloride gas was bubbled into a mixture of 110 g (2.00 mole) of propionitrile and 70.0 g (2.19 mole) of methanol while cooling with an ice bath and maintaining the reaction mixture under a nitrogen stream until 78.5 g (2.15 mole) of hydrogen chloride had been added.
  • the reaction flask was stoppered and stirred at 20° C. for 4.5 days.
  • To this mixture was added 150 ml of methanol.
  • Ammonia gas was bubbled into the mixture (accompanied by cooling) for two hours until the mixture was basic to litmus paper.
  • the flask was stoppered and stirred for about 16 hours. The solids were removed by filtration, washed with methanol and the washings and filtrate were concentrated by evaporation.
  • a mixture of 150 g (1.15 mole) of 4,6-dihydroxy-2-ethylpyrimidine and 1073 g (7.0 mole, 640 ml) of phosphorus oxychloride was heated at reflux for 6 hours, cooled and evaporated in vacuo to provide a brown oil as the residue.
  • the residue was poured into 1500 ml of an ice-water mixture.
  • the mixture was extracted thrice with 400 ml portions of diethyl ether.
  • the combined ether extracts were washed sequentially with water (200 ml), 5% sodium hydroxide solution (twice with 200 ml portions) and saturated sodium chloride solution (200 ml) and were then dried over magnesium sulfate.
  • Example 1 Using the method of Part E, Example 1, the indicated intermediate amines of Formula VIA were reacted with the indicated known 4-chloro-2-alkyl-6-hydrazinylpyrimidines of Formula VI to provide the novel intermediates of Formula VIII (Table I).
  • Example 1 Using the method of Part F, Example 1, the indicated intermediates of Formula VIII were reacted with the indicated trialkyl orthoesters to provide the novel compounds of Formula IX (Table II).
  • Example 1 Using the method of Part G, Example 1, the indicated intermediates of Formula IX were heated with formic acid to provide the indicated compounds of Formula I (Table III).
  • Example 97 Using the method of Example 97, the indicated intermediates of Formula VIII were reacted with the indicated orthoesters to provide the indicated compounds of Formula I (Table IV).
  • Example 104 Using the method of Example 104, the indicated intermediates of Formula VIII were reacted with the indicated orthoesters to provide the indicated compounds of Formula I (Table V).
  • Example 128, Part A the indicated intermediate of Formula V was reacted with the indicated amine of Formula VIA to provide the indicated intermediate of Formula VII.
  • the intermediate of Formula VII was then reacted with hydrazine hydrate in accordance with the method of Example 128, Part B, to provide the indicated intermediate of Formula VIII (Table VI).
  • Example 132 Part B
  • the indicated intermediates of Formula XII were prepared from the indicated intermediate of Formula XI (Table VIII).
  • the structures of the intermediates of Formula XII were confirmed by infrared and nuclear magnetic resonance spectral analyses.
  • Example 132 Part C
  • the indicated compounds of Formula I were prepared from the indicated intermediates of Formula XII and the indicated amine of Formula VIA (Table IX). Chromatographic separations were used to obtain the desired [1,5-c]isomers.
  • Example 142 Using the method of Example 142, the indicated compounds of Formula I were prepared from the indicated intermediates of Formula XIV and the indicated amines of Formula VIA (Table X).

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Abstract

1,2,4-Triazolo[1,5-c]pyrimidines substituted at the 5 or 7 positions through a nitrogen atom which is part of a heterocyclic ring have been found to have potent bronchodilator activity. Methods for inducing bronchodilation, pharmaceutical compositions and synthetic processes are also disclosed.

Description

TECHNICAL FIELD
The present invention relates to triazolo[1,5-c]pyrimidines, and more specifically to 1,2,4-triazolo[1,5-c]pyrimidines. The pharmacological use of the compounds of the invention as bronchodilators, pharmaceutical compositions comprising the compounds, and processes for the preparation of the compounds are also within the scope of the invention.
BACKGROUND OF THE INVENTION
Some 1,2,4-triazolo[1,5-c]pyrimidines are known to the art. Certain 1,2,4-triazolo[1,5-c]pyrimidines are disclosed as being bronchodilators in the patents discussed below, the compounds being referred to therein as triazolo[2,3-c]pyrimidines:
United Kingdom Patent No. 859,287 discloses 2-amino-1,2,4-triazolo[1,5-c]pyrimidines which are substituted on the pyrimidine ring at the 5, 7 and 8 positions by certain combinations of substituents selected from hydrogen, alkyl, halogen-substituted alkyl, hydroxy-substituted alkyl, alkoxy-substituted alkyl, alkenyl, cycloalkyl, amino, alkylamino, dialkylamino, phenyl, alkylthio, alkoxy and halogen substituents. United Kingdom Patent No. 898,407 discloses processes for preparing certain of these compounds by subjecting the corresponding 1,2,4-triazolo[4,3-c]pyrimidines to an acid treatment, to an alkaline treatment, or to a heat treatment.
United Kingdom Patent No. 873,223 discloses 2-amino or 2-acetamido-1,2,4-triazolo[1,5-c]pyrimidines which are substituted on the pyrimidine ring at the 5, 7 and 8 positions by certain combinations of substituents selected from hydrogen, alkyl, halogen-substituted alkyl, alkoxy-substituted alkyl, alkenyl, cycloalkyl, alkylthio and halogen substituents.
United Kingdom Patent No. 897,870 discloses 2-alkylamino-1,2,4-triazolo[1,5-c]pyrimidines, 2-dialkylamino-1,2,4-triazolo[1,5-c]pyrimidines, and 1,2,4-triazolo[1,5-c]pyrimidines containing a piperidino or morpholino substituent bonded at the 2-position through the nitrogen atom, which compounds are substituted on the pyrimidine ring at the 5, 7 and 8 positions by certain combinations of substituents selected from hydrogen, alkyl, halogen-substituted alkyl, hydroxy-substituted alkyl, alkenyl and halogen substituents.
The following related articles discloses the synthesis of certain 1,2,4-triazolo[1,5-c]pyrimidines as potential bronchodilators:
G. W. Miller et al., J. Chem. Soc., 1963, 5642, discloses 2-amino- or 2-acetamido-1,2,4-triazolo[1,5-c]pyrimidines (referred to therein as triazolo[2,3-c]pyrimidines) which are substituted on the pyrimidine ring by, for example, hydrogen and alkyl substituents. Certain of these compounds are said to be bronchodilators.
G. W. Miller et al., J. Chem. Soc., 1963, 3357, discloses 1,2,4-triazolo[1,5-c]pyrimidines (referred to therein as triazolo[2,3-c]pyrimidines) which are substituted at the 2-position by hydroxy, halogen, alkoxy, amino or substituted amino substituents and on the pyrimidine ring by alkyl substituents, or alkyl and halogen-substituted alkyl substituents.
W. Broadbent et al., J. Chem. Soc., 1963, 3369, discloses 1,2,4-triazolo[1,5-c]pyrimidines (referred to therein as triazolo[2,3-c]pyrimidines) which are substituted at the 2-position by a mercapto, alkylthio, alkylsulphonyl, or dialkylamino substituent, and on the pyrimidine ring by alkyl substituents or alkyl and halogen-substituted alkyl substituents.
Still other 1,2,4-triazolo[1,5-c]pyrimidines are disclosed in the following articles and patents.
Temple et al., J. Org. Chem., 1963, 33, 530, discloses the compound 8-amino-7-chloro-s-triazolo[1,5-c]pyrimidine-2(3H)-one.
D. J. Brown et al., Aust. J. Chem., 1978, 31, 2505, discloses 1,2,4-triazolo[1,5-c]pyrimidines which are substituted at the 2-position by hydrogen or an alkyl substituent, and on the pyrimidine ring by hydrogen and/or alkyl substituents.
D. J. Brown et al., Aust. J. Chem., 1979, 32, 1585, discloses 1,2,4-triazolo[1,5-c]pyrimidines which are substituted at the 2-position by hydrogen or an alkyl substituent, and on the pyrimidine ring at the 5-position by a halogen, hydrazino, alkyl or alkylthio substituent, and at the 7-position by an alkyl substituent.
D. J. Brown et al., Aust. J. Chem., 1980, 33, 1147, discloses pyrimidines which are substituted at the 2-position by hydrogen or an alkyl or phenyl substituent, and on the pyrimidine ring at the 5-position by halogen, dimethylaminomethyleneamino, hydroxyaminomethyleneamino or 5-acetoxyaminomethyleneamino, and at the 7-position by hydrogen or an alkyl substituent.
U.S. Pat. No. 4,269,980 discloses 5-, 7- and 8-(optionally substituted phenyl)-1,2,4-triazolo[1,5-c]pyrimidines. These compounds may be substituted at the 2-position by hydrogen or an alkyl substituent and are anxiolytic agents.
DETAILED DESCRIPTION OF THE INVENTION
The present invention relates to 1,2,4-triazolo[1,5-c]pyrimidines which are bronchodilators. The invention also relates to a method for inducing bronchodilation in a mammal using a 1,2,4-triazolo[1,5,-c]pyrimidine of the invention, and to pharmaceutical compositions comprising an effective amount of a 1,2,4-triazolo[1,5-c]pyrimidine of the invention and a pharmaceutically acceptable carrier. The invention also relates to synthetic processes useful for preparing the 1,2,4-triazolo[1,5-c]pyrimidines of the invention.
Specifically, the present invention relates to compounds of the formula I ##STR1## wherein R2 is hydrogen or lower alkyl; at least one of R5 and R7 is ##STR2## wherein each X is independently oxygen, sulfur, sulfinyl, sulfonyl, methylene (--CH2 --), imido (--NH--) or N-lower alkylimido ##STR3## and one of R5 and R7 may also be hydrogen, lower alkyl or phenyl; and R8 is hydrogen, lower alkyl or phenyl; and pharmaceutically acceptable salts thereof.
"Lower alkyl" as used in the instant specification and claims designates straight or branched-chain alkyl groups containing one to about four carbon atoms. Preferred lower alkyl substituents are methyl and ethyl.
The presently preferred compounds of the invention are those wherein X is sulfur or oxygen and R8 is hydrogen. These compounds are preferred because of their generally higher potency in protecting against histamineinduced contraction of isolated guinea pig tracheal tissue. This assay is discussed in greater detail below.
Specific examples of preferred compounds which are active in the aforementioned assay at concentrations of 5 ug per ml or lower are:
2-methyl-5-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine
2-ethyl-7-methyl-5-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine
5,7-bis(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine
5-(4-morpholino)-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine
2-ethyl-5-methyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine
2-ethyl-5-methyl-7-(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine
2,5-diethyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine
2-ethyl-7-(4-morpholino)-5-n-propyl-1,2,4-triazolo[1,5-c]pyrimidine
7-(4-morpholino)-5-n-propyl-1,2,4-triazolo[1,5-c]pyrimidine
2-ethyl-5-n-propyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine
5-n-propyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine
5-ethyl-7-(4-morpholino)-1,2,4-triazlo[1,5-c]pyrimidine
5-ethyl-2-methyl-7-(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine
2,5-diethyl-7-(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine
The bronchodilator activity of the compounds of Formula I was assessed by the measurement of effects on isolated tracheal spirals. This is a well-known and long established in vitro test method. The bronchodilator activity was determined according to the following procedure: Female guinea pigs were sacrificed and each trachea removed and cut into a spiral strip. This strip was mounted in a constant temperature (37° C.) muscle bath having a volume of approximately 15 ml. The bathing medium was Krebs-Henseleit solution. Movement of the tracheal strip was measured by means of an isometric transducer connected to an electric recorder. The bath was aerated with a mixture of 95% carbon dioxide and 5% oxygen. Contractions were induced in the strips by the addition of a suitable amount of histamine, acetylcholine or barium chloride. The amount of a given compound of Formula I (measured in ug/ml) required to provide greater than 75% relaxation of drug-induced contraction is considered an effective concentration. For comparison, a well known standard bronchodilator, aminophylline, requires concentrations of 50 ug/ml versus histamine, 100 ug/ml versus acetylcholine and 10 ug/ml versus barium chloride to provide greater than 75% relaxation.
The compounds of Formula I which were most active in the in vitro test, including most of those listed above as preferred compounds, were tested in vivo in the guinea pig for oral activity in the so-called histamine aerosol method described in U.S. Pat. No. 3,248,292. This test was modified slightly in that a 0.1% aqueous solution of histamine was used as the agent for inducing bronchial constriction. Oral doses were measured in mg/kg of body weight of the guinea pig.
Some of the compounds of Formula I were also found to have activity as mucolytics in an in vitro test for mucus production in which rats are orally dosed with compound prior to sacrifice. The trachea is then isolated and incubated with radiolabelled glucosamine and the effect of compounds on the incorporation of glucosamine into extracellular mucus is determined. An active compound reduces incorporation of glucosamine. Specific examples of preferred compounds which are active in this assay are:
7-Methyl-5-(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine
2,7-Dimethyl-5-(4-methyl-1-piperazinyl)-1,2,4-triazolo[1,5-c]pyrimidine
2,7-Dimethyl-5-(1-piperazinyl)-1,2,4-triazolo[1,5-c]pyrimidine.
The compounds of Formula I may be administered to mammals in order to obtain bronchodilation. The compounds may be administered orally, parenterally or by inhalation. Preferably they are administered orally in tablets or capsules. The usual effective human dose will be in the range of 0.1 to 50 mg/kg of body weight.
Salts of compounds of Formula I are generally prepared by reaction with an equimolar amount of a relatively strong acid, preferably an inorganic acid such as hydrochloric, sulfuric or phosphoric acid, in a polar solvent. Isolation of the salt is facilitated by the addition of a solvent in which the salt is insoluble, an example of such a solvent is diethyl ether.
The compounds of Formula I, either as the free base or in the form of a pharmaceutically acceptable acidaddition salt, can be combined with conventional pharmaceutical diluents and carriers to form such dosage forms as tablets, capsules, suspensions, solutions, suppositories and the like.
The pharmaceutical carrier employed may be, for example, either a solid or liquid. Examples of solid carriers are lactose, terra alba, sucrose, talc, gelatin, agar, pectin, acacia, magnesium stearate, stearic acid, and the like. Liquid carriers include syrup, peanut oil, olive oil, water and the like. Similarly, the carrier or diluent can include a time delay material well known to the art, such as glyceryl monostearate or glyceryl distearate, these being employed alone or, for example, in combination with a wax.
The compounds of Formula I may be prepared by several synthetic routes. One such route is that shown in Scheme I below. This route is useful in preparing compounds wherein R5 is hydrogen, lower alkyl or phenyl; R7 is ##STR4## and R2, R8 and X are as defined previously. Each "alk" appearing in Scheme I is independently lower alkyl. ##STR5##
The reactions of steps (1), (2) and (3) have previously been reported for the preparation of compounds wherein R5 is hydrogen, methyl or ethyl and R8 is hydrogen or methyl. Thus, most of the compounds of formulas IV, V, and VI are known. Heterocyclic compounds of formulas VII, VIII and IX are novel. The known methods were used to carry out the reactions of steps (1), (2) and (3). Specifically, steps (1) and (2) were carried out as described in H. R. Henze et al., J. Org. Chem., 1952, 17, 1320 and H. R. Henze et al., J. Org. Chem., 1953, 18, 653, and step (3) was carried out as described in J. Chesterfield et al., J. Chem. Soc., 1955, 3478. Example 1 hereinbelow details these steps.
Step (4) is carried out by reacting the optionally substituted 4-chloro-6-hydrazinopyrimidine of Formula VI with a heterocyclic amine of the formula VIA. The reactants are heated together without solvent or optionally (and preferably) in any solvent which does not participate in the reaction such as water. Two equivalents of the heterocyclic amine are preferably used. Alternatively, one equivalent of the heterocyclic amine may be replaced by an inorganic base to neutralize the hydrogen chloride, but lower yields are obtained. The reaction mixture is heated at a temperature up to or at its reflux temperature. A temperature is chosen which provides an adequate reaction rate. When water is used as the solvent, the temperature is generally in the range of 80° to 110° C. Good yields of the desired products are isolated by conventional methods such as filtration, extraction or chromatography. The novel intermediate of Formula VIII, which may also be prepared alternatively by following steps (5) and (6), are solids whose structural assignments are confirmed by infrared and nuclear magnetic resonance spectral analyses.
Step (5) is carried out by reacting optionally substituted 4,6-dichloropyrimidines of Formula V with heterocyclic amines of the formula VIA. This reaction is carried out by heating the reactants without solvent, or preferably in any solvent which does not participate in the reaction. Two equivalents of the heterocyclic amine are preferably used, one to react with the chloropyrimidine and the other to neutralize the hydrogen chloride by-product. Alternatively, an inorganic base may be used to neutralize the hydrogen chloride by-product, but lower yields of the desired product are generally obtained. Heating is at a temperature up to and including the reflux temperature of the mixture. A temperature is chosen which provides an adequate reaction rate. If water is used as a solvent, the mixture is generally heated at its reflux temperature. Good yields of the desired product are isolated by conventional methods such as filtration, extraction or chromatography. The novel intermediates of Formula VII are solids. Structural assignments are confirmed by infrared and nuclear magnetic resonance spectral analyses.
Step (6) is carried out by reacting the novel substituted 4-chloro-6-heterocyclicaminopyrimidine of Formula VII with hydrazine hydrate. The reaction is facile and is generally carried out at moderate temperatures, for example, from -20° C. to the reflux temperature of the solvent. The reaction is generally carried out by adding two equivalents of hydrazine hydrate to a solution of the pyrimidine. The solvent will generally be a lower alkanol. The product is separated by conventional methods such as filtration, extraction or chromatography and is the same novel intermediate of Formula VIII obtained from step (4).
Step (7) is carried out by reacting the intermediate of Formula VIII with an orthoester of formula VIIA. Such orthoesters are well known and readily available. Examples of suitable orthoesters include trimethyl orthoformate, triethyl orthoformate, triethyl orthoacetate, triethyl orthopropionate and the like. Since the orthoesters are liquids, it is convenient to mix the intermediates of Formula VIII with an excess of orthoester and to heat the mixture at reflux until reaction is complete. Good yields of the desired solid intermediates of Formula IX which are novel substituted 1,2,4-triazolo[4,3-c]pyrimidines are obtained by conventional methods.
In step (8), the 1,2,4-triazolo[4,3-c]pyrimidines of Formula IX are heated with an aqueous acid and thereby converted to the desired compounds of the invention of Formula I wherein R5 is ##STR6## The preferred aqueous acids are carboxylic acids such as formic acid, acetic acid and propionic acid. The reaction mixture is generally heated at reflux for up to several days. The desired product is isolated by conventional methods. The structural assignments are made based on infrared and nuclear magnetic resonance spectral analyses. The products are generally white crytalline solids.
In some cases step (8) may be accomplished by continued heating of the reactants of step (7). This conversion occurs most readily when R5 is hydrogen, and is carried out by using dimethyl sulfoxide as the solvent for the combined steps (7) and (8) as described in Example 104.
Synthetic Scheme II shows a method for the preparation of compounds of Formula I wherein both R5 and R7 are ##STR7## wherein X is as defined previously and may be the same or different in the two heterocyclic amino groups; and R2 and R8 are as defined previously. Alk is as defined previously. PG,13 ##STR8##
Step (1) of Scheme II requires reaction of the 4-chloro-6-hydrazino-2-methylthiopyrimidine of Formula X with a heterocyclic amine of the Formula VIA. Generally the 4-chloro-6-hydrazino-2-methylthiopyrimidines of Formula X are known compounds or may be prepared by conventional methods. The reaction is generally conducted in an inert solvent, preferably water, optionally in the presence of an acid acceptor such as a tertiary organic amine, for example, triethylamine. The reaction is best carried out using two equivalents of the amine of Formula VIA, one to react with the 4-chloro-6-hydrazino-2-methylthiopyrimidine and one to react with the hydrochloric acid which results. The mixture is heated at reflux for several hours, then cooled. Usually the novel intermediate of Formula XI is obtained directly as a solid precipitate. Alternatively, it is obtained by extraction or chromatographic techniques.
Step (2) of Scheme II is carried out by mixing the intermediate of Formula XI with an orthoester of Formula VIIIA. The reaction is carried out as described for step (7) of Scheme I. The product obtained is a novel intermediate of Formula XII. The product of this step generally is a mixture which is separated by chromatography, preferably high pressure liquid chromatography, to provide the crystalline solid. Occasionally the desired isomer is obtained in such high purity that chromatographic separation is unnecessary.
Step (3) of Scheme II requires heating of the intermediate of Formula XII in an excess of the heterocyclic amine of the Formula VIA, optionally in an inert solvent such as diglyme or dioxane. The reaction is generally carried out at the reflux temperature of the mixture. The product is isolated by conventional methods such as filtration, extraction or chromatography.
An alternative scheme, used for preparing compounds of Formula I wherein R5 is ##STR9## wherein X is as defined previously; R7 is hydrogen or lower alkyl; and R2 and R8 are as defined previously, is shown in Scheme III. ##STR10##
The compounds of Formula XIV are generally known or may be prepared by conventional methods. Known methods may be employed to vary substituents R2 and R7. The reaction of Scheme III occurs readily at moderate temperatures, for example from 0° C. up to the reflux temperature of the solvent. It is carried out either by adding the heterocyclic amine of Formula VIA to a solution of the intermediate of Formula XIV or vice versa. The solvent may be inert solvent, for example water or dioxane. The product of Formula I prepared in Scheme III is obtained in good yields by conventional isolation methods.
Synthetic Scheme IV illustrated below is a method for preparation of compounds of Formula I wherein R2 and R7 are independently hydrogen or lower alkyl; R5 is ##STR11## wherein X is as defined previously; and R8 is as defined previously. ##STR12##
The reaction of Scheme IV is similar to reaction of step (3) of Scheme II. The intermediates of Formula XV are known or may be prepared from known starting materials using known methods. The products of Scheme IV are prepared and isolated as described for that step.
The following examples are provided to illustrate the methods used in the invention. They are not intended to limit the invention.
EXAMPLE 1 Preparation of 2,7-Diethyl-5-(4-methyl-1-piperazinyl)-1,2,4-triazolo[1,5-c]pyrimidine according to Scheme I, Steps (1), (2), (3), (4), (7) and (8) Part A1 Preparation of Propionamidine
Hydrogen chloride gas was bubbled into a mixture of 110 g (2.00 mole) of propionitrile and 70.0 g (2.19 mole) of methanol while cooling with an ice bath and maintaining the reaction mixture under a nitrogen stream until 78.5 g (2.15 mole) of hydrogen chloride had been added. The reaction flask was stoppered and stirred at 20° C. for 4.5 days. To this mixture was added 150 ml of methanol. Ammonia gas was bubbled into the mixture (accompanied by cooling) for two hours until the mixture was basic to litmus paper. The flask was stoppered and stirred for about 16 hours. The solids were removed by filtration, washed with methanol and the washings and filtrate were concentrated by evaporation. The residue was dissolved in 400 ml of ethanol. The solution was cooled and then filtered and the filtrate was concentrated by evaporation. The residue was again dissolved in ethanol, cooled, filtered and the filtrate was evaporated to provide a residue which crystallized to provide 114 g (53%) of propionamidine hydrochloride.
Part A2 Alternative Preparation of Propionamidine
A mixture of 35 g (0.2 mole) of triethyl orthopropionate and 15.4 g (0.2 mole) of ammonium acetate was reacted as described by Taylor, et. al., J. Am. Chem. Soc., 1960, 82, 3138, by heating the mixture at reflux for 45 minutes. The product was isolated by distilling off ethanol, followed by filtration to provide 17 g of propionamidine acetate, m.p. 165°-167° C. An additional 3 g was obtained by concentrating the filtrate and adding acetone to provide a 77% yield overall. The structure was confirmed by nuclear magnetic resonance and infrared spectral analyses.
Part B1 Preparation of 4,6-Dihydroxy-2-ethylpyrimidine according to Step (1)
To a cooled (using an ice-bath), stirred solution of 25% sodium methoxide (1200 ml, 5.55 mole) was added a slurry of 200 g (1.85 mole) of propionamidine hydrochloride in 300 ml methanol. Next, 244 g (1.85 mole) of dimethyl malonate was added and the mixture was permitted to warm to about 20° C., after which it was stirred for 16 hours. The mixture was evaporated in vacuo and water (about 2 l) was then added. This mixture was neutralized with concentrated hydrochloric acid to provide a white precipitate which was separated by filtration to provide 230 g (96%) of 4,6-dihydroxy-2-ethylpyrimidine, m.p. 312°-315° C. The structural assignment was confirmed by infrared and nuclear magnetic resonance spectral analyses.
Part B2 Alternative Preparation of 4,6-Dihydroxy-2-ethylpyrimidine
Using the procedure of Example 1, Part B1, 18.5 g (0.14 mole) of propionamidine acetate and 90 ml of 25% sodium methoxide were reacted with dimethyl malonate (0.14 mole) to provide 16.5 g (89.5%) of 4,6-dihydroxy-2-ethylpyrimidine according to Step (1).
Part C Preparation of 4,6-Dichloro-2-ethylpyrimidine
A mixture of 150 g (1.15 mole) of 4,6-dihydroxy-2-ethylpyrimidine and 1073 g (7.0 mole, 640 ml) of phosphorus oxychloride was heated at reflux for 6 hours, cooled and evaporated in vacuo to provide a brown oil as the residue. The residue was poured into 1500 ml of an ice-water mixture. The mixture was extracted thrice with 400 ml portions of diethyl ether. The combined ether extracts were washed sequentially with water (200 ml), 5% sodium hydroxide solution (twice with 200 ml portions) and saturated sodium chloride solution (200 ml) and were then dried over magnesium sulfate. Evaporation of the ether provided an oil which was distilled to provide 41 g (70%) of 4,6-dichloro-2-ethylpyrimidine, b.p. 55-60 C/1.5 to 4 mm Hg. The structural assignment was confirmed by infrared and nuclear magnetic resonance spectral analyses.
Part D Preparation of 4-Chloro-2-ethyl-6-hydrazinylpyrimidine according to Step (3)
To a stirred, cold (0° C.) solution of 60 g (0.34 mole) of 4,6-dichloro-2-ethylpyrimidine in 500 ml of methanol was added 35 g (0.7 mole) of hydrazine hydrate while maintaining the temperature below 10° C. Stirring was continued for 2 hours after completion of the addition, the temperature being maintained at 0° C. The mixture was allowed to warm to about 20° C. and was stirred for 16 hours. The white solid was collected by filtration and the filtrate was partially evaporated to provide a second crop. The combined solids were washed with water and air dried to provide 51 g (77%) of 4-chloro-2-ethyl-6-hydrazinylpyrimidine, m.p. 147°-150° C.
Part E Preparation of 2-Ethyl-6-hydrazinyl-4-(4-methyl-1-piperazinyl)pyrimidine according to Step (4)
A mixture of 8.3 g (0.05 mole) of 4-chloro-2-ethyl-6-hydrazinylpyrimidine and 11 g (0.10 mole) of 1-methylpiperazine in 250 ml of water was heated at reflux for 16 hours and was then cooled and extracted with chloroform. The extracts were dried over magnesium sulfate and were then evaporated to provide 8.3 g (70%) of 2-ethyl-6-hydrazinyl-4-(4-methyl-1-piperazinyl)pyrimidine.
Part F Preparation of 3,5-Diethyl-7-(4-methyl-1-piperazinyl)-1,2,4-triazolo[4,3-c]pyrimidine according to Step (7)
A mixture of 8.3 g (0.045 mole) of 2-ethyl-6-hydrazinyl-4-(4-methyl-1-piperazinyl)pyrimidine and 75 ml of triethyl orthopropionate was heated at reflux for 48 hours. After cooling the mixture was evaporated in vacuo. Diethyl ether was added to the residue and the mixture was cooled. The precipitate was collected by filtration to provide 4.5 g (37%) of 3,5-diethyl-7-(4-methyl-1-piperazinyl)-1,2,4-triazolo[4,3-c]pyrimidine. Recrystallization from ethyl acetate-hexane and then from ethyl acetate-cyclohexane provided a white crystalline product, m.p. 128°-131° C. Analysis for C14 H22 N6 : Calculated: %C, 61.3; %H, 8.1; %N, 30.6; Found: %C, 59.8; %H, 8.2; %N, 30.1. The structural assignment was confirmed by nuclear magnetic resonance and infrared spectral analyses.
Part G Preparation of 2,5-Diethyl-7-(4-methyl-1-piperazinyl)-1,2,4-triazolo[1,5-c]pyrimidine according to step (8)
A mixture of 3.3 g of 3,5-diethyl-7-(4-methyl-1-piperazinyl)-1,2,4-triazolo[4,3-c]pyrimidine and 50 ml of 97% formic acid was heated at reflux for 18 hours. The mixture was cooled and evaporated in vacuo to provide a residue which was diluted with 100 ml of water and carefully neutralized with sodium bicarbonate. The solution was extracted with chloroform. The extracts were dried and then concentrated to provide an oil which solidified and was collected by filtration, washed with water and dried. Recrystallization from hexane provided off-white crystalline solid 2,5-diethyl-7-(4-methyl-1-piperazinyl)-1,2,4-triazolo[1,5-c]pyrimidine, m.p. 87°-88° C. Analysis: Calculated for C14 H22 N6 : %C, 61.3; %H, 8.1; %N, 30.6; Found: %C, 60.6; %H, 8.1, %N, 30.6. The structural assignment was confirmed by infrared and nuclear magnetic resonance spectral analyses.
EXAMPLES 2-21
Using the method of Part E, Example 1, the indicated intermediate amines of Formula VIA were reacted with the indicated known 4-chloro-2-alkyl-6-hydrazinylpyrimidines of Formula VI to provide the novel intermediates of Formula VIII (Table I).
                                  TABLE I                                 
__________________________________________________________________________
            Pyrimidine                                                    
     Amine  Intermediate                    Melting Point                 
Example                                                                   
     Intermediate                                                         
            of Formula VI                                                 
                         Intermediate of Formula VIII                     
                                            (in °C.)               
__________________________________________________________________________
 2                                                                        
      ##STR13##                                                           
             ##STR14##                                                    
                          ##STR15##         none taken (yield 66%)        
 3                                                                        
      ##STR16##                                                           
            "                                                             
                          ##STR17##         158-160 (yield 94.5%)         
 4                                                                        
      ##STR18##                                                           
            "                                                             
                          ##STR19##         129-133 (yield 75%)           
 5                                                                        
      ##STR20##                                                           
             ##STR21##                                                    
                          ##STR22##         112-115                       
 6                                                                        
      ##STR23##                                                           
             ##STR24##                                                    
                          ##STR25##         none taken (yield 84%)        
 7                                                                        
      ##STR26##                                                           
             ##STR27##                                                    
                          ##STR28##         144-146 (yield 71%)           
 8                                                                        
      ##STR29##                                                           
             ##STR30##                                                    
                          ##STR31##         none taken (yield 85%)        
 9                                                                        
      ##STR32##                                                           
             ##STR33##                                                    
                          ##STR34##         none taken (yield 68%)        
10                                                                        
      ##STR35##                                                           
             ##STR36##                                                    
                          ##STR37##         183-185                       
11                                                                        
      ##STR38##                                                           
             ##STR39##                                                    
                          ##STR40##         none taken (yield 66%)        
12                                                                        
      ##STR41##                                                           
             ##STR42##                                                    
                          ##STR43##         203-204                       
13                                                                        
      ##STR44##                                                           
             ##STR45##                                                    
                          ##STR46##         174-177                       
14                                                                        
      ##STR47##                                                           
             ##STR48##                                                    
                          ##STR49##         155-157                       
15                                                                        
      ##STR50##                                                           
             ##STR51##                                                    
                          ##STR52##         147-149                       
16                                                                        
      ##STR53##                                                           
             ##STR54##                                                    
                          ##STR55##         122-123                       
17                                                                        
      ##STR56##                                                           
             ##STR57##                                                    
                          ##STR58##         124-126                       
18                                                                        
      ##STR59##                                                           
             ##STR60##                                                    
                          ##STR61##         none taken                    
19                                                                        
      ##STR62##                                                           
             ##STR63##                                                    
                          ##STR64##         none taken                    
20                                                                        
      ##STR65##                                                           
             ##STR66##                                                    
                          ##STR67##         (oil)                         
21                                                                        
      ##STR68##                                                           
             ##STR69##                                                    
                          ##STR70##         (oil)                         
__________________________________________________________________________
EXAMPLES 22-59
Using the method of Part F, Example 1, the indicated intermediates of Formula VIII were reacted with the indicated trialkyl orthoesters to provide the novel compounds of Formula IX (Table II).
                                  TABLE II                                
__________________________________________________________________________
Intermediate                                    Calculated: % C; % H; %   
                                                N                         
of Formula VIII            Intermediate of Formula IX                     
                                                Found: % C; % H; % N      
Ex.                                                                       
   R.sub.5 R.sub.8                                                        
              X  Ortho Ester                                              
                           R.sub.2                                        
                                  R.sub.5 R.sub.8                         
                                             X  (m.p. in                  
__________________________________________________________________________
                                                °C.)               
22 CH.sub.3                                                               
           H  SO.sub.2                                                    
                 triethyl  H      CH.sub.3                                
                                          H  SO.sub.2                     
                                                44.9; 4.9; 26.2           
                 orthoformate                   44.9; 4.9; 26.3           
                                                (299-300)                 
23 CH.sub.3                                                               
           H  SO.sub.2                                                    
                 triethyl  CH.sub.3                                       
                                  CH.sub.3                                
                                          H  SO.sub.2                     
                                                47.0; 5.4; 25.0           
                 orthoacetate                   47.1; 5.4; 24.9           
                                                (307-308)                 
24 CH.sub.2 CH.sub.2 CH.sub.3                                             
           H  O  triethyl  H      CH.sub.2 CH.sub.2 CH.sub.3              
                                          H  O  58.3; 6.9; 28.3           
                 orthoformate                   58.2; 7.0; 28.4           
                                                (200-202)                 
25 CH.sub. 2 CH.sub.2 CH.sub.3                                            
           H  O  triethyl  CH.sub.2 CH.sub.3                              
                                  CH.sub.2 CH.sub.2 CH.sub.3              
                                          H  O  61.1; 7.7; 25.4           
                 orthopropionate                60.9; 7.9; 25.7           
                                                (179-181)                 
26 CH.sub.2 CH.sub.2 CH.sub.3                                             
           H  S  triethyl  H      CH.sub.2 CH.sub.2 CH.sub.3              
                                          H  S  54.7; 6.5; 26.6           
                 orthoformate                   54.6; 6.5; 26.9           
                                                (208-209)                 
27 CH.sub.2 CH.sub.2 CH.sub.3                                             
           H  S  triethyl  CH.sub.2 CH.sub.3                              
                                  CH.sub.2 CH.sub.2 CH.sub.3              
                                          H  S  57.7; 7.3; 24.0           
                 orthopropionate                57.7; 7.5; 24.4           
                                                (143-144)                 
28 CH.sub.2 CH.sub.2 CH.sub.3                                             
           H  CH.sub.2                                                    
                 triethyl  CH.sub.2 CH.sub.3                              
                                  CH.sub.2 CH.sub.2 CH.sub.3              
                                          H  CH.sub.2                     
                                                (as H.sub.2 SO.sub.4      
                                                salt):                    
                 orthopropionate                48.5; 6.8; 18.9           
                                                48.4; 7.0; 19.0           
                                                (190-192)                 
29 CH.sub.3                                                               
           H  S  triethyl  H      CH.sub.3                                
                                          H  S  51.0; 5.6; 29.8           
                 orthoformate                   51.0; 5.5; 29.6           
                                                (234-237)                 
30 CH.sub.2 CH.sub.3                                                      
           H  CH.sub.2                                                    
                 triethyl  CH.sub.2 CH.sub.3                              
                                  CH.sub.2 CH.sub.3                       
                                          H  CH.sub.2                     
                                                64.8; 8.2; 27.0           
                 orthopropionate                65.1; 8.3; 27.2           
                                                (120-122)                 
31 CH.sub.2 CH.sub.3                                                      
           H  S  triethyl  CH.sub.2 CH.sub.3                              
                                  CH.sub.2 CH.sub.3                       
                                          H  S  56.3; 6.9; 25.2           
                 orthopropionate                56.1; 7.2; 25.5           
                                                (149-150)                 
32 CH.sub.2 CH.sub.3                                                      
           H  S  triethyl  CH.sub.3                                       
                                  CH.sub.2 CH.sub.3                       
                                          H  S  54.7; 6.5; 26.6           
                 orthoacetate                   54.4; 6.7; 26.7           
                                                (168-170)                 
33 CH.sub.2 CH.sub.3                                                      
           H  S  triethyl  H      CH.sub.2 CH.sub.3                       
                                          H  S  53.0; 6.0; 28.1           
                 orthoformate                   52.6; 6.2; 28.2           
                                                (243-245)                 
34 CH.sub.2 CH.sub.3                                                      
           H  O  triethyl  CH.sub.3                                       
                                  CH.sub.2 CH.sub.3                       
                                          H  O  58.3; 6.9; 28.3           
                 orthoacetate                   58.2; 7.0; 28.5           
                                                (200-203)                 
35 CH.sub.2 CH.sub.3                                                      
           H  O  triethyl  CH.sub.2 CH.sub.3                              
                                  CH.sub.2 CH.sub.3                       
                                          H  O  59.7; 7.3; 26.8           
                 orthopropionate                59.6; 7.4; 27.0           
                                                (173-174)                 
36 CH.sub.2 CH.sub.3                                                      
           H  O  triethyl  H      CH.sub.2 CH.sub.3                       
                                          H  O  56.6; 6.5; 30.0           
                 orthoformate                   56.6; 6.4; 30.3           
                                                (223-224)                 
37 CH.sub.3                                                               
           H  O  trimethyl H      CH.sub.3                                
                                          H  O  54.8; 6.0; 31.9           
                 orthoformate                   54.7; 6.0; 31.4           
                                                (208-210)                 
38 CH.sub.3                                                               
           H  O  triethyl  CH.sub.3                                       
                                  CH.sub.3                                
                                          H  O  56.6; 6.5; 30.0           
                 orthoacetate                   56.7; 6.3; 30.3           
                                                (171-173)                 
39 CH.sub.3                                                               
           H  O  triethyl  CH.sub.2 CH.sub.3                              
                                  CH.sub.3                                
                                          H  O  58.3; 6.9; 28.3           
                 orthopropionate                57.9; 7.0; 28.3           
                                                (169-172)                 
40 CH.sub.3                                                               
           H  S  triethyl  CH.sub.3                                       
                                  CH.sub.3                                
                                          H  S  53.0; 6.0; 28.1           
                 orthoacetate                   53.1; 6.3; 28.3           
                                                (223-225)                 
41 CH.sub.3                                                               
           H  S  triethyl  CH.sub.2 CH.sub.3                              
                                  CH.sub.3                                
                                          H  S  54.8; 6.5; 26.6           
                 orthopropionate                54.4; 6.7; 26.8           
                                                (161-163)                 
42 CH.sub.3                                                               
           CH.sub.3                                                       
              S  triethyl  H      CH.sub.3                                
                                          CH.sub.3                        
                                             S  53.0; 6.0; 28.1           
                 orthoformate                   53.2; 6.3; 28.4           
                                                (182-184)                 
43 CH.sub.3                                                               
           CH.sub.3                                                       
              S  triethyl  CH.sub.3                                       
                                  CH.sub.3                                
                                          CH.sub.3                        
                                             S  54.7; 6.5; 26.6           
                 orthoacetate                   54.4; 6.6; 26.9           
                                                (184-185)                 
44 CH.sub.3                                                               
           CH.sub.3                                                       
              S  triethyl  CH.sub.2 CH.sub.3                              
                                  CH.sub.3                                
                                          CH.sub.3                        
                                             S  56.3; 6.9; 25.2           
                 orthopropionate                56.4; 7.1; 25.1           
                                                (129-130)                 
45 CH.sub.3                                                               
           CH.sub.3                                                       
              SO.sub.2                                                    
                 triethyl  H      CH.sub.3                                
                                          CH.sub.3                        
                                             SO.sub.2                     
                                                46.9; 5.4; 24.9           
                 orthoformate                   47.0; 5.3; 25.2           
                                                (234-236)                 
46 CH.sub.2 CH.sub.3                                                      
           H  S  trimethyl CH.sub.2 CH.sub.2 CH.sub.3                     
                                  CH.sub.2 CH.sub.3                       
                                          H  S  57.7; 7.3; 24.0           
                 orthobutyrate                  57.6; 7.5; 24.2           
                                                (163-165)                 
47 CH.sub.2 CH.sub.3                                                      
           H  O  trimethyl CH.sub.2 CH.sub.2 CH.sub.3                     
                                  CH.sub.2 CH.sub.3                       
                                          H  O  61.1; 7.7; 25.4           
                 orthobutyrate                  61.0; 7.9; 25.8           
                                                (164-166)                 
48 CH.sub.2 CH.sub.3                                                      
           H  O  trimethyl CH(CH.sub.3 ).sub.2                            
                                  CH.sub.2 CH.sub.3                       
                                          H  O  61.1; 7.7; 25.4           
                 orthoisobutyrate               61.0; 7.7; 25.3           
                                                (136-137)                 
49 CH.sub.2 CH.sub.3                                                      
           H  S  trimethyl CH(CH.sub.3).sub.2                             
                                  CH.sub.2 CH.sub.3                       
                                          H  S  57.7; 7.3; 24.0           
                 orthoisobutyrate               57.8; 7.3; 24.2           
                                                (143-144)                 
50 CH.sub.2 CH.sub.2 CH.sub.3                                             
           H  S  trimethyl CH.sub.2 CH.sub.2 CH.sub.3                     
                                  CH.sub.2 CH.sub.2 CH.sub.3              
                                          H  S  59.0; 7.6; 22.9           
                 orthobutyrate                  59.0; 7.5; 23.1           
                                                (134-135)                 
51 CH.sub.2 CH.sub.2 CH.sub.3                                             
           H  O  trimethyl CH.sub.2 CH.sub.2 CH.sub.3                     
                                  CH.sub.2 CH.sub.2 CH.sub.3              
                                          H  O  62.3; 8.0; 24.2           
                 orthobutyrate                  62.2; 8.1; 24.1           
                                                (139-140)                 
52 CH.sub.2 CH.sub.2 CH.sub.3                                             
           H  S  trimethyl CH(CH.sub.3).sub.2                             
                                  CH.sub.2 CH.sub.2 CH.sub.3              
                                          H  S  59.0; 7.6; 22.9           
                 orthoisobutyrate               59.1; 7.7; 23.0           
                                                (134-136)                 
53 CH.sub.2 CH.sub.2 CH.sub.3                                             
           H  O  trimethyl CH(CH.sub.3).sub.2                             
                                  CH.sub.2 CH.sub.2 CH.sub.3              
                                          H  O  62.3; 8.0; 24.2           
                 orthoisobutyrate               62.4; 8.1; 24.6           
                                                (116-117)                 
54 CH(CH.sub.3).sub.2                                                     
           H  O  triethyl  H      CH(CH.sub.3).sub.2                      
                                          H  O  (as H.sub.2 SO.sub.4      
                                                Salt):                    
                 orthoformate                   41.7; 5.5; 20.3           
                                                41.6; 5.7; 20.6           
                                                (177-178)                 
55 CH(CH.sub.3).sub.2                                                     
           H  O  triethyl  CH.sub.2 CH.sub.3                              
                                  CH(CH.sub.3).sub.2                      
                                          H  O  (as H.sub.2 SO.sub.4      
                                                Salt):                    
                 orthopropionate                45.0; 6.2; 18.8           
                                                44.9; 6.3; 19.0           
                                                (167-168)                 
56 CH(CH.sub.3).sub.2                                                     
           H  O  trimethyl CH.sub. 2 CH.sub.2 CH.sub.3                    
                                  CH(CH.sub.3).sub.2                      
                                          H  O  (as H.sub.2 SO.sub.4      
                                                Salt):                    
                 orthobutyrate                  46.5; 6.5; 18.1           
                                                46.2; 6.5; 18.3           
                                                (162-163)                 
57 CH(CH.sub.3).sub.2                                                     
           H  S  triethyl  CH.sub.3                                       
                                  CH(CH.sub.3).sub.2                      
                                          H  S  (as H.sub.2 SO.sub.4      
                                                Salt):                    
                 orthoacetate                   41.6; 5.6; 18.7           
                                                41.6; 5.8; 18.8           
                                                (170-172)                 
58 CH(CH.sub.3).sub.2                                                     
           H  S  triethyl  CH.sub.2 CH.sub.3                              
                                  CH(CH.sub.3).sub.2                      
                                          H  S  (as H.sub.2 SO.sub.4      
                                                Salt):                    
                 orthopropionate                43.2; 6.0; 18.0           
                                                43.1; 6.1; 18.2           
                                                (174-175)                 
59 CH(CH.sub.3).sub.2                                                     
           H  O  triethyl  H      CH(CH.sub.3).sub.2                      
                                          H  O  (as H.sub.2 SO.sub.4      
                                                Salt):                    
                 orthoformate                   41.7; 5.5; 20.3           
                                                41.6; 5.7; 20.6           
                                                (177-178)                 
__________________________________________________________________________
EXAMPLES 60-96
Using the method of Part G, Example 1, the indicated intermediates of Formula IX were heated with formic acid to provide the indicated compounds of Formula I (Table III).
              TABLE III                                                   
______________________________________                                    
                          Product of Formula I                            
     Product of Formula IX;                                               
                          Calculated: % C;                                
Ex.  Product of Formula I % H; % N Found:                                 
No.  R.sub.2   R.sub.5   R.sub.8                                          
                             X    % C; % H; % N                           
______________________________________                                    
60   CH.sub.2 CH.sub.3                                                    
               CH.sub.2 CH.sub.3                                          
                         H   CH.sub.2                                     
                                  64.8; 8.2; 27.0                         
                                  65.1; 8.3; 27.0                         
                                  m.p. 90-92° C.                   
61   CH.sub.2 CH.sub.3                                                    
               CH.sub.2 CH.sub.3                                          
                         H   S    56.3; 6.9; 25.2                         
                                  56.1; 7.1; 25.0                         
                                  m.p. 119-120° C.                 
62   CH.sub.3  CH.sub.2 CH.sub.3                                          
                         H   S    54.7; 6.5; 26.6                         
                                  54.8; 6.8; 26.5                         
                                  m.p. 164-165° C.                 
63   H         CH.sub.2 CH.sub.3                                          
                         H   S    53.0; 6.0; 28.1                         
                                  52.9; 6.3; 28.4                         
                                  m.p. 228-230° C.                 
64   CH.sub.3  CH.sub.2 CH.sub.3                                          
                         H   O    58.3; 6.9; 28.3                         
                                  58.3; 7.0; 28.3                         
                                  m.p. 159-160° C.                 
65   CH.sub.2 CH.sub.3                                                    
               CH.sub.2 CH.sub.3                                          
                         H   O    59.7; 7.3; 26.8                         
                                  59.7; 7.3; 26.9                         
                                  m.p. 118-119° C.                 
66   H         CH.sub.2 CH.sub.3                                          
                         H   O    56.6; 6.5; 30.0                         
                                  56.8; 6.5; 30.1                         
                                  m.p. 168-170° C.                 
67   H         CH.sub.3  H   O.sub.2 S                                    
                                  44.9; 4.9; 26.2                         
                                  44.9; 4.9; 26.2                         
                                  m.p. 251-253° C.                 
68   CH.sub.3  CH.sub.3  H   O.sub.2 S                                    
                                  47.0; 5.4; 25.0                         
                                  46.8; 5.4; 24.4                         
                                  m.p. 229-233° C.                 
69   H         (CH.sub.2).sub.2 CH.sub.3                                  
                         H   O    58.3; 6.9; 28.3                         
                                  58.5; 7.0; 28.6                         
                                  m.p. 153-155° C.                 
70   CH.sub.2 CH.sub.3                                                    
               (CH.sub.2).sub.2 CH.sub.3                                  
                         H   O    61.1; 7.7; 25.4                         
                                  61.2; 7.7; 25.6                         
                                  m.p. 130-131° C.                 
71   H         (CH.sub. 2).sub.2 CH.sub.3                                 
                         H   S    54.7; 6.5; 26.6                         
                                  54.9; 6.5; 26.9                         
                                  m.p. 145-150° C.                 
72   CH.sub.2 CH.sub.3                                                    
               (CH.sub.2).sub.2 CH.sub.3                                  
                         H   S    57.7; 7.3; 24.0                         
                                  57.6; 7.3; 24.2                         
                                  m.p. 101-103° C.                 
73   CH.sub.2 CH.sub.3                                                    
               (CH.sub.2).sub.2 CH.sub.3                                  
                         H   CH.sub.2                                     
                                  65.9; 8.5; 25.6                         
                                  65.8; 8.6; 25.8                         
                                  m.p. 60-62° C.                   
74   H         CH.sub.3  H   S    51.0; 5.5; 29.8                         
                                  51.0; 5.7; 30.2                         
                                  m.p. 154-156° C.                 
75   H         CH.sub.3  H   O    54.8; 6.0; 31.9                         
                                  54.7; 5.8; 32.4                         
                                  m.p. 159-161.5° C.               
76   CH.sub.3  CH.sub.3  H   O    56.5; 6.5; 30.0                         
                                  56.7; 6.6; 30.6                         
                                  m.p. 182-183° C.                 
77   CH.sub.2 CH.sub.3                                                    
               CH.sub.3  H   O    58.3; 6.9; 28.3                         
                                  58.3; 7.0; 28.7                         
                                  m.p. 146-147° C.                 
78   CH.sub.3  CH.sub.3  H   S    53.0; 6.0; 28.1                         
                                  52.7; 6.1; 28.0                         
                                  m.p. 170-172° C.                 
79   CH.sub.2 CH.sub.3                                                    
               CH.sub.3  H   S    54.7; 6.5; 26.7                         
                                  54.6; 6.7; 27.1                         
                                  m.p. 124-125° C.                 
80   H         CH.sub.3  CH.sub.3                                         
                             S    53.0; 6.0; 28.1                         
                                  52.9; 6.2; 28.4                         
                                  m.p. 150-152° C.                 
81   CH.sub.3  CH.sub.3  CH.sub.3                                         
                             S    54.7; 6.5; 26.2                         
                                  54.8; 6.5; 26.6                         
                                  m.p. 128-130° C.                 
82   CH.sub.2 CH.sub.3                                                    
               CH.sub.3  CH.sub.3                                         
                             S    56.3; 6.9; 25.2                         
                                  56.3; 6.9; 25.4                         
                                  m.p. 80-81° C.                   
83   H         CH.sub.3  CH.sub.3                                         
                             O.sub.2 S                                    
                                  46.9; 5.4; 24.9                         
                                  46.9; 5.3; 25.1                         
                                  m.p. 219-224° C.                 
84   CH(CH.sub.3).sub.2                                                   
               CH.sub.2 CH.sub.3                                          
                         H   S    (as H.sub.2 SO.sub. 4 Salt):            
                                  43.7; 6.0; 18.0                         
                                  43.1; 6.0; 18.4                         
                                  m.p 193-194° C.                  
85   CH(CH.sub.3).sub.2                                                   
               CH.sub.2 CH.sub.3                                          
                         H   O    (as H.sub.2 SO.sub.4 Salt):             
                                  45.0; 6.2; 18.8                         
                                  44.9; 6.3; 19.1                         
                                  m.p. 189-190° C.                 
86   (CH.sub.2).sub.2 CH.sub.3                                            
               CH.sub.2 CH.sub.3                                          
                         H   O    (as H.sub.2 SO.sub.4 Salt):             
                                  45.0; 6.2; 18.8                         
                                  44.9; 6.3; 19.1                         
                                  m.p. 170-172° C.                 
87   (CH.sub.2).sub.2 CH.sub.3                                            
               CH.sub.2 CH.sub.3                                          
                         H   S    (as H.sub.2 SO.sub.4 Salt):             
                                  43.2; 6.0; 18.0                         
                                  43.0; 6.1; 18.3                         
                                  m.p. 152-153° C.                 
88   (CH.sub.2).sub.2 CH.sub.3                                            
               CH.sub.3 (CH.sub.2).sub.2                                  
                         H   S    (as H.sub.2 SO.sub.4 Salt):             
                                  44.6; 6.2; 17.4                         
                                  44.7; 6.4; 17.5                         
                                  m.p. 143-145° C.                 
89   (CH.sub.2).sub.2 CH.sub.3                                            
               (CH.sub.2).sub.2 CH.sub.3                                  
                         H   O    (as H.sub.2 SO.sub.4 Salt):             
                                  46.5; 6.5; 18.1                         
                                  46.6; 6.7; 18.4                         
                                  m.p. 169-171° C.                 
90   CH(CH.sub.3).sub.2                                                   
               (CH.sub.2).sub.2 CH.sub.3                                  
                         H   S    (as H.sub.2 SO.sub.4 Salt):             
                                  44.6; 6.2; 17.4                         
                                  44.8; 6.4; 17.6                         
                                  m.p. 172-173° C.                 
91   CH(CH.sub.3).sub.2                                                   
               CH.sub.3 (CH.sub.2).sub.2                                  
                         H   O    (as H.sub.2 SO.sub.4 Salt):             
                                  46.5; 6.5; 18.1                         
                                  46.4; 6.7; 18.3                         
                                  m.p. 156-157° C.                 
92   CH.sub.2 CH.sub.3                                                    
               CH(CH.sub.3).sub.2                                         
                         H   O    61.1; 7.7; 25.4                         
                                  61.0; 7.9; 25.9                         
                                  m.p. 128-129° C.                 
93   (CH.sub.2).sub.2 CH.sub.3                                            
               CH(CH.sub.3).sub.2                                         
                         H   O    62.3; 8.0; 24.2                         
                                  61.3; 8.1; 24.0                         
                                  m.p. 85-86° C.                   
94   CH.sub.3  CH(CH.sub.3).sub.2                                         
                         H   S    56.3; 6.9; 25.3                         
                                  56.1; 6.9; 25.8                         
                                  m.p. 170-172° C.                 
95   CH.sub.2 CH.sub.3                                                    
               (CH.sub.3).sub.2 CH                                        
                         H   S    57.7; 7.3; 24.0                         
                                  57.3; 7.1; 24.3                         
                                  m.p. 142-143° C.                 
96   H         CH(CH.sub.3).sub.2                                         
                         H   O    (as H.sub.2 SO.sub.4 Salt):             
                                  41.7; 5.5; 20.3                         
                                  41.0; 5.2; 20.3                         
                                  m.p. 179-182° C.                 
______________________________________
EXAMPLE 97 Preparation of 2-n-Propyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine
A mixture of 8.00 g (37.9 mmole) of 4-hydrazino-6-(4-thiomorpholino)pyrimidine and 20 ml of trimethyl ortho-n-butyrate was heated at its reflux temperature for about 60 hours, cooled, and the solid was isolated by filtration. The product was washed with diethyl ether, then recrystallized twice from a benzene-hexane mixture accompanied by treatment with decolorizing charcoal to provide off-white 2-n-propyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine, m.p. 137°-138° C. Most (4.6 g, 17.5 mmole) of this product was dissolved in 100 ml of ethanol and 1.75 g (17.1 mmole) of sulfuric acid was added. The solution was diluted to 300 ml with diethyl ether and allowed to stand for two hours. The precipitate was collected by filtration, washed with diethyl ether and dried to provide the dihydrogen sulfate salt, m.p. 134°-137° C. Analysis: Calculated for C12 H17 N5 S.H2 SO4 : %C, 39.9; %H, 5.3; %N, 19.4; Found: %C, 39.5; %H, 5.2; %N, 19.5. The structural assignment was confirmed by infrared spectral analysis.
EXAMPLES 98-103
Using the method of Example 97, the indicated intermediates of Formula VIII were reacted with the indicated orthoesters to provide the indicated compounds of Formula I (Table IV).
                                  TABLE IV                                
__________________________________________________________________________
     Inter-                                                               
     mediate                                                              
     of Formula                  Calculated: % C; % H; % N                
Example                                                                   
     VIII           Product of Formula I                                  
                                   Found: % C; % H; % N                   
Number                                                                    
     R.sub.5                                                              
       R.sub.8                                                            
         X Orthoester                                                     
                    R.sub.2                                               
                           R.sub.5                                        
                             R.sub.8                                      
                               X (m.p. in C)                              
__________________________________________________________________________
 98  H H O trimethyl                                                      
                    CH(CH.sub.3).sub.2                                    
                           H H O 58.3; 6.9; 28.3                          
           orthoisobutyrate      58.1; 6.9; 28.5                          
                                 (154-155)                                
 99  H H S triethyl CH.sub.3                                              
                           H H S 51.0; 5.6; 29.8                          
           orthoacetate          50.9; 5.5; 30.1                          
                                 (145-148)                                
100  H H S triethyl CH.sub.2 CH.sub.3                                     
                           H H S 53.0; 6.1; 28.1                          
           orthopropionate       52.7; 6.0; 28.3                          
                                 (125-126)                                
101  H H S trimethyl                                                      
                    CH.sub.2 CH.sub.2 CH.sub.3                            
                           H H S 54.7; 6.5; 26.6                          
           orthobutyrate         54.6; 6.4; 26.7                          
                                 (137-138)                                
102  H H O triethyl H      H H O 52.7; 5.4; 34.1                          
           orthoformate          52.2; 5.3; 34.2                          
                                 (202-204)                                
103  H H O triethyl CH.sub.2 CH.sub.3                                     
                           H H O 56.6; 6.5; 30.0                          
           orthopropionate       56.5; 6.3; 30.2                          
                                 (164-165)                                
__________________________________________________________________________
EXAMPLE 104 Preparation of 2,5-Di(isopropyl)-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine
A mixture of 4.00 g (15.8 mmole) of 4-hydrazino-2-isopropyl-6-(4-thiomorpholino)pyrimidine, 2.40 g (16.2 mmole) of trimethyl isobutyrate, 0.95 g (15.8 mmole) of acetic acid and 40 ml of dimethyl sulfoxide was heated at 120° C. for about 65 hours and then poured into 200 ml of ice water. The mixture was basified with 10 percent aqueous sodium hydroxide and extracted four times with 50 ml of chloroform. The extracts were washed six times with 150 ml of water, dried over magnesium sulfate and evaporated to provide a dark oil. Nuclear magnetic resonance spectral analysis indicated that the oil was chiefly the desired product. The product was chromatographed on 70 g of silica gel, eluting sequentially with 600 ml of dichloromethane, one liter of 50:50 ethyl acetate:dichloromethane, and ethyl acetate, 100 ml fractions being taken. Fractions 7, 8 and 9 provided 3.35 g (70%) of brown oil. The oil was dissolved in 50 ml of ethanol, and sulfuric acid (1.09 g, 10.7 mmole) and 200 ml of diethyl ether were then added sequentially. The precipitate was collected by filtration, washed with diethyl ether and dried. The product was off-white solid 2,5-di(isopropyl)-7-(4-thiomorpholino)-1,2,4-triazolo-[1,5-c]pyrimidine dihydrogen sulfate, m.p. 192°-194° C. Analysis: Calculated for C15 H23 N5 S.H2 SO4 : %C, 44.6; %H, 6.3; %N, 17.4; Found: %C, 44.6; %H, 6.3; %N, 17.5. The structure was confirmed by infrared and nuclear magnetic resonance spectral analyses.
EXAMPLES 105-127
Using the method of Example 104, the indicated intermediates of Formula VIII were reacted with the indicated orthoesters to provide the indicated compounds of Formula I (Table V).
                                  TABLE V                                 
__________________________________________________________________________
     Intermediate                               Calculated: % C; % H; %   
                                                N                         
Example                                                                   
     of Formula VIII       Product of Formula I Found: % C; % H; % N      
Number                                                                    
     R.sub.5 R.sub.8                                                      
               X Orthoester                                               
                           R.sub.2  R.sub.5 R.sub.8                       
                                              X (m.p. in                  
__________________________________________________________________________
                                                C.)                       
105  CH.sub.2 CH.sub.3                                                    
             H S trimethyl CH.sub.2 CH(CH.sub.3).sub.2                    
                                    CH.sub.2 CH.sub.3                     
                                            H S (as H.sub.2 SO.sub.4      
                                                Salt):                    
                 orthoisovalerate               44.6; 6.2; 17.4           
                                                44.5; 6.5; 17.6           
                                                (155-156)                 
106  CH(CH.sub.3).sub.2                                                   
             H S trimethyl CH.sub.2 CH(CH.sub.3).sub.2                    
                                    CH(CH.sub.3).sub.2                    
                                            H S (as H.sub.2 SO.sub.4      
                                                Salt):                    
                 orthoisovalerate               46.0; 6.5; 16.8           
                                                45.9; 6.7; 16.9           
                                                (169-170)                 
107  CH.sub.2 CH.sub.3                                                    
             H S trimethyl (CH.sub.2).sub.3 CH.sub.3                      
                                    CH.sub.2 CH.sub.3                     
                                            H S (as H.sub.2 SO.sub.4      
                                                Salt):                    
                 orthovalerate                  44.6; 6.2; 17.4           
                                                44.3; 6.2; 17.7           
                                                (132-135)                 
108  (CH.sub.2).sub.2 CH.sub.3                                            
             H S trimethyl (CH.sub.2).sub.3 CH.sub.3                      
                                    (CH.sub.2).sub.2 CH.sub.3             
                                            H S (as H.sub.2 SO.sub.4      
                                                Salt):                    
                 orthovalerate                  46.0; 6.5; 16.8           
                                                46.0; 6.4; 17.1           
                                                (152-153)                 
109  CH.sub.2 CH(CH.sub.3).sub.2                                          
             H S trimethyl CH(CH.sub.3).sub.2                             
                                    CH.sub.2 CH(CH.sub.3).sub.2           
                                            H S 60.1; 7.9; 21.9           
                 orthoisobutyrate               59.7; 7.9; 21.7           
                                                (94-97)                   
110  CH.sub.2 CH(CH.sub.3).sub.2                                          
             H O triethyl  CH.sub.2 CH.sub.3                              
                                    CH.sub.2 CH(CH.sub.3).sub.2           
                                            H O 62.2; 8.0; 24.2           
                 orthopropionate                62.0; 7.7; 24.2           
                                                (119-122)                 
111  CH.sub.2 CH(CH.sub.3).sub.2                                          
             H O trimethyl CH(CH.sub.3).sub.2                             
                                    CH.sub.2 CH(CH.sub.3).sub.2           
                                            H O 63.3; 8.3; 23.1           
                 orthoisobutyrate               63.2; 8.0; 23.1           
                                                (93-95)                   
112  CH.sub.2 CH(CH.sub.3).sub.2                                          
             H O trimethyl (CH.sub.2).sub.3 CH.sub.3                      
                                    CH.sub.2 CH(CH.sub.3).sub.2           
                                            H O 64.3; 8.6; 22.1           
                 orthovalerate                  63.7; 8.2; 21.9           
                                                (85-88)                   
113  (CH.sub.2).sub.3 CH.sub.3                                            
             H S triethyl  CH.sub.3 (CH.sub.2).sub.3 CH.sub.3             
                                            H S 57.7; 7.7; 24.0           
                 orthoacetate                   57.3; 7.3; 24.1           
                                                (123-126)                 
114  (CH.sub.2).sub.3 CH.sub.3                                            
             H S trimethyl (CH.sub.2).sub.2 CH.sub.3                      
                                    (CH.sub.2).sub.3 CH.sub.3             
                                            H S 60.3; 7.9; 22.0           
                 orthobutyrate                  59.9; 8.0; 21.9           
                                                (66-68)                   
115  (CH.sub.2).sub.3 CH.sub.3                                            
             H S trimethyl CH.sub.2 CH(CH.sub.3).sub.2                    
                                    (CH.sub.2).sub.3 CH.sub.3             
                                            H S 61.4; 8.2; 21.1           
                 orthoisovalerate               60.9; 8.3; 21.1           
                                                (50-53)                   
116  CH.sub.2 CH(CH.sub.3).sub.2                                          
             H O triethyl  H        CH.sub.2 CH(CH.sub.3).sub.2           
                                            H O 59.7; 7.3; 26.8           
                 orthoformate                   59.6; 7.2; 26.9           
                                                (123-126)                 
117  (CH.sub.2).sub.3 CH.sub.3                                            
             H S triethyl  CH.sub.2 CH.sub.3                              
                                    (CH.sub.2).sub.3 CH.sub.3             
                                            H S 59.0; 7.6; 22.9           
                 orthopropionate                59.2; 7.7; 23.3           
                                                (110-112)                 
118  CH.sub.2 CH.sub.3                                                    
             H O trimethyl (CH.sub.2).sub.3 CH.sub.3                      
                                    CH.sub.2 CH.sub.3                     
                                            H O 62.3; 8.0; 24.2           
                 orthovalerate                  62.1; 8.0; 24.3           
                                                (87-89)                   
119  (CH.sub.2).sub.2 CH.sub.3                                            
             H O trimethyl (CH.sub.2).sub.3 CH.sub.3                      
                                    (CH.sub.2).sub.2 CH.sub.3             
                                            H O 63.3; 8.3; 23.1           
                 orthovalerate                  63.1; 8.2; 23.3           
                                                (105-106)                 
120  (CH.sub.2).sub.3 CH.sub.3                                            
             H O triethyl  CH.sub.3 (CH.sub.2).sub.3 CH.sub.3             
                                            H O 61.1; 7.7; 25.4           
                 orthoacetate                   61.0; 7.8; 25.3           
                                                (132-133)                 
121  (CH.sub.2).sub.3 CH.sub.3                                            
             H O triethyl  CH.sub.2 CH.sub.3                              
                                    (CH.sub.2).sub.3 CH.sub.3             
                                            H O 62.3; 8.0; 24.2           
                 orthopropionate                62.1; 8.1; 24.2           
                                                (105-106)                 
122  (CH.sub.2).sub.3 CH.sub.3                                            
             H O trimethyl (CH.sub.2).sub.2 CH.sub.3                      
                                    (CH.sub.2).sub.3 CH.sub.3             
                                            H O 63.3; 8.3; 23.1           
                 orthobutyrate                  63.3; 8.4; 23.2           
                                                (98- 99)                  
123  (CH.sub.2).sub.3 CH.sub.3                                            
             H O trimethyl CH.sub.2 CH(CH.sub.3).sub.2                    
                                    (CH.sub.2).sub.3 CH.sub.3             
                                            H O 64.3; 8.6; 22.1           
                 orthoisovalerate               64.2; 8.4; 22.0           
                                                (73-75)                   
124  CH.sub.2 CH(CH.sub.3).sub.2                                          
             H S triethyl  CH.sub.2 CH.sub.3                              
                                    CH.sub.2 CH(CH.sub.3).sub.2           
                                            H S 59.0; 7.6; 22.9           
                 orthopropionate                59.0; 7.2; 23.1           
                                                (124-126)                 
125  (CH.sub.2).sub.2 CH.sub.3                                            
             H O triethyl  CH.sub.2 CH(CH.sub.3).sub.2                    
                                    (CH.sub.2).sub.2 CH.sub.3             
                                            H O 63.3; 8.3; 23.1           
                 orthoisovalerate               63.1; 7.9; 23.2           
                                                (53-55)                   
126  CH.sub.2 CH(CH.sub.3).sub.2                                          
             H S triethyl  H        CH.sub.2 CH(CH.sub.3).sub.2           
                                            H S 56.3; 6.9; 25.2           
                 orthoformate                   56.3; 6.9; 25.4           
                                                (134-136)                 
127  CH.sub.2 CH.sub.3                                                    
             H O trimethyl CH.sub.2 CH(CH.sub.3).sub.2                    
                                    CH.sub.2 CH.sub.3                     
                                            H O 62.3; 8.0; 24.2           
                 orthoisovalerate               62.3; 7.9; 24.3           
                                                (50-52)                   
__________________________________________________________________________
EXAMPLE 128 Part A Preparation of 4-Chloro-2-methyl-6-(4-morpholino)pyrimidine by Scheme I, Step (5)
A solution of 5.00 g (31.7 mmole) of 4,6-dichloro-2-methylpyrimidine and 6.00 g (68.9 mmole) of morpholine in 50 ml of water was heated on a steam cone for about 18 hours. The mixture was diluted with water and cooled. The resulting white solid was separated by filtration, washed with water and dried to provide 4.84 g (72%) of 4-chloro-2-methyl-6-(4-morpholino)pyrimidine. The structural assignment was confirmed by infrared and nuclear magnetic resonance spectral analyses.
Part B Prepartion of 4-Hydrazino-2-methyl-6-(4-morpholino)pyrimidine by Scheme I, Step (6)
To a mixture of 4.70 g (22 mmole) of 4-chloro-2-methyl-6-(4-morpholino)pyrimidine in 50 ml of ethanol was added 2.2 g (44 mmole) of hydrazine hydrate and the mixture was heated at its reflux temperature for 16 hours. Cooling provided a precipitate which was separated by filtration and washed with ethanol to provide 3.15 g (68%) of white solid 4-hydrazino-2-methyl-6-(4-morpholino)pyrimidine. The structural assignment of the product was confirmed by infrared and nuclear magnetic resonance spectral analyses and comparison with the same compound made in Example 9.
EXAMPLES 129-131
Using the method of Example 128, Part A, the indicated intermediate of Formula V was reacted with the indicated amine of Formula VIA to provide the indicated intermediate of Formula VII. The intermediate of Formula VII was then reacted with hydrazine hydrate in accordance with the method of Example 128, Part B, to provide the indicated intermediate of Formula VIII (Table VI).
                                  TABLE VI                                
__________________________________________________________________________
Example                                                                   
     Intermediate                                                         
               Heterocyclic                                               
                      Intermediate    Intermediate                        
Number                                                                    
     of Formula V                                                         
               Amine  of Formula VII  of Formula VIII                     
__________________________________________________________________________
129                                                                       
      ##STR71##                                                           
                ##STR72##                                                 
                       ##STR73##                                          
                                       ##STR74##                          
                      m.p. 147.5-150° C., White Solid              
                                      White Solid                         
130                                                                       
      ##STR75##                                                           
                ##STR76##                                                 
                       ##STR77##                                          
                                       ##STR78##                          
                      m.p. 210-220° C., White Solid                
                                      m.p. 232-234° C., White      
                                      Solid                               
131                                                                       
      ##STR79##                                                           
                ##STR80##                                                 
                       ##STR81##                                          
                                       ##STR82##                          
                      m.p. about 200° C.                           
                                      m.p. 250-253° C., White      
__________________________________________________________________________
                                      Solid
EXAMPLE 132 Preparation According to Scheme II of 5,7-Bis(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine Part A Preparation of 4-Hydrazinyl-2-methylthio-6-(4-morpholino)pyrimidine according to Scheme II, Step (1)
To a solution of 3.0 g (15.7 mmole) of 4-chloro-6-hydrazinyl-2-methylthiopyrimidine in 50 ml of water was added 2.8 g (32.2 mmole) of morpholine, and the mixture was heated at reflux for two days. Cooling gave a precipitate which was separated by filtration, washed with water and dried to provide off-white solid 4-hydrazinyl-2-methylthio-6-(4-morpholino)pyrimidine, m.p. 134°-144° C. The structural assignment was confirmed by infrared and nuclear magnetic resonance spectral analyses.
Part B Preparation of 5-Methylthio-7-(4-morpholino)-1,2,4-triazolo[4,3-c]pyrimidine according to Scheme II, Step (2)
A mixture of 24.75 g (103 mmole) of 4-hydrazinyl-2-methylthio-6-(4-morpholino)pyrimidine and 200 ml of triethyl orthoformate was heated at 120° C. in an open flask for 60 hours. The mixture was cooled, then diluted with 300 ml of diethyl ether. The precipitate was separated by filtration, washed with ether and dried to provide 5-methylthio-7-(4-morpholino)-1,2,4-triazolo[4,3-c]pyrimidine, m.p. 212°-213° C. after two recrystallizations from chloroform-hexane.
Part C Preparation of 5,7-Bis(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine according to Scheme II, Step (3)
A mixture of 7.10 g (28.3 mmole) of 5-methylthio-7-(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine and 35 ml of morpholine was heated at reflux for 16 hours. Cooling of the mixture provided a solid. The mixture was diluted to a total volume of 100 ml with diethyl ether, cooled and the product separated by filtration. Recrystallization with treatment with decolorizing charcoal from a mixture of benzene and hexane (1:1) provided white solid 5,7-bis(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine, m.p. 176°-177° C. Analysis: Calculated for C13 H18 N6 O2 : %C, 53.8; %H, 6.2; %N, 29.0; Found: %C, 53.8; %H, 6.1; %N, 28.9. The structural assignment was confirmed by infrared and nuclear magnetic resonance spectral analyses.
EXAMPLES 133-135
Using the method of Example 132, Part A, the indicated intermediates of Formula XI were prepared from 4-chloro-6-hydrazino-2-methylthiopyrimidine and the indicated amine of Formula VIA (Table VII). The structures of the intermediates of Formula XI were confirmed by infrared and nuclear magnetic resonance spectral analyses.
              TABLE VII                                                   
______________________________________                                    
Ex-                                                                       
am-  Amine Reactant                                                       
                  Intermediate                                            
ple  of Formula VIA                                                       
                  of Formula XI                                           
______________________________________                                    
133                                                                       
      ##STR83##                                                           
                   ##STR84##                                              
134                                                                       
      ##STR85##                                                           
                   ##STR86##                                              
135                                                                       
      ##STR87##                                                           
                   ##STR88##                                              
______________________________________
EXAMPLES 136-138
Using the method of Example 132, Part B, the indicated intermediates of Formula XII were prepared from the indicated intermediate of Formula XI (Table VIII). The structures of the intermediates of Formula XII were confirmed by infrared and nuclear magnetic resonance spectral analyses.
              TABLE VIII                                                  
______________________________________                                    
Ex-   Intermediate of                                                     
                  Intermediate of                                         
ample Formula XI  Formula XII                                             
______________________________________                                    
136   Example 133                                                         
                   ##STR89##                                              
137   Example 134                                                         
                   ##STR90##                                              
138   Example 135                                                         
                   ##STR91##                                              
______________________________________
EXAMPLES 139-141
Using the method of Example 132, Part C, the indicated compounds of Formula I were prepared from the indicated intermediates of Formula XII and the indicated amine of Formula VIA (Table IX). Chromatographic separations were used to obtain the desired [1,5-c]isomers.
                                  TABLE IX                                
__________________________________________________________________________
     Intermediate                                                         
             Amine of                 Calculated: % C; % H; % N           
Example                                                                   
     of Formula XII                                                       
             Formula VIA                                                  
                    Product of Formula I                                  
                                      Found: % C; % H; %                  
__________________________________________________________________________
                                      N                                   
139  Example 136                                                          
              ##STR92##                                                   
                     ##STR93##        55.4; 7.0; 32.3 55.9; 6.9; 31.8     
                                      (m.p. 142-143.5° C.)         
140  Example 137                                                          
              ##STR94##                                                   
                     ##STR95##        51.1; 6.3; 32.1 51.5; 6.3; 31.8     
                                      (m.p. 127-130° C.)           
141  Example 138                                                          
              ##STR96##                                                   
                     ##STR97##        51.0; 5.9; 27.4 50.7; 5.9; 27.6     
                                      (m.p. 169-170° C.)           
__________________________________________________________________________
EXAMPLE 142 The Preparation of 2,7-Dimethyl-5-(1-piperazino)-1,2,4-triazolo[1,5-c]pyrimidine according to Scheme III
A solution of the known compound 5-chloro-2,7-dimethyl-1,2,4-triazolo[1,5-c]pyrimidine (2.0 g, 11 mmole) in 50 ml of dioxane was added dropwise to a suspension of 120 g (140 mmole) of piperazine in 75 ml of dioxane. After stirring for 3 hours at 20° C., the mixture was diluted with 150 ml of water and then extracted with three 150 ml portions of chloroform. The extracts were washed with two 150 ml portions of water and two 100 ml portions of saturated sodium chloride solution and were then dried over magnesium sulfate. The extracts were evaporated to provide a residue which was recrystallized with treatment with decolorizing charcoal from a benzene-hexane mixture (1:3) to provide white solid 2,7-dimethyl-5-(1-piperazino)-1,2,4-triazolo[1,5-c]pyrimidine, m.p. 108°-110° C. Analysis: Calculated for C11 H16 N6 : %C, 56.9; %H, 6.9; %N, 36.2; Found: %C, 57.0; %H, 6.9; %N, 35.6.
EXAMPLES 143-156
Using the method of Example 142, the indicated compounds of Formula I were prepared from the indicated intermediates of Formula XIV and the indicated amines of Formula VIA (Table X).
                                  TABLE X                                 
__________________________________________________________________________
Intermediate                                Calculated: % C; % H; % N     
of Formula XIV Amine of Product of Formula I                              
                                            Found: % C; % H; % N          
Example                                                                   
     R.sub.2                                                              
          R.sub.7                                                         
             R.sub.8                                                      
               Formula VIA                                                
                        R.sub.2                                           
                             R.sub.5   R.sub.7                            
                                          R.sub.8                         
                                            (m.p. in C.)                  
__________________________________________________________________________
143  CH.sub.3                                                             
          CH.sub.3                                                        
             H                                                            
                ##STR98##                                                 
                        CH.sub.3                                          
                              ##STR99##                                   
                                       CH.sub.3                           
                                          H 58,5; 7.4; 34.1 58.8; 7.3;    
                                            34.8 (106.5-107.5)            
144  CH.sub.3                                                             
          CH.sub.3                                                        
             H                                                            
                ##STR100##                                                
                        CH.sub.3                                          
                              ##STR101##                                  
                                       CH.sub.3                           
                                          H 47.0; 5.4; 24.9 47.0; 5.3;    
                                            25.4 (201-203)                
145  CH.sub.3                                                             
          CH.sub.3                                                        
             H                                                            
                ##STR102##                                                
                        CH.sub.3                                          
                              ##STR103##                                  
                                       CH.sub.3                           
                                          H 49.8; 5.7; 26.4 50.0; 5.6;    
                                            26.8 (186-188)                
146  CH.sub.3                                                             
          CH.sub.3                                                        
             H                                                            
                ##STR104##                                                
                        CH.sub.3                                          
                              ##STR105##                                  
                                       CH.sub.3                           
                                          H 53.0; 6.1; 28.1 53.0; 6.1;    
                                            28.7 (133-134)                
147  CH.sub.3                                                             
          CH.sub.3                                                        
                ##STR106##                                                
                        CH.sub.3                                          
                              ##STR107##                                  
                                       CH.sub.3                           
                                          H 56.6; 6,5; 30.0 56.6; 6.4;    
                                            30.5 (142-144)                
148  H    CH.sub.3                                                        
                ##STR108##                                                
                        H                                                 
                              ##STR109##                                  
                                       CH.sub.3                           
                                          H 51.0; 5.6; 29.8 50.7; 5.6;    
                                            29.8 (99.5-100)               
149  H    CH.sub.3                                                        
                ##STR110##                                                
                        H                                                 
                              ##STR111##                                  
                                       CH.sub.3                           
                                          H 48.7; 5.2; 27.9 48.1; 5.2;    
                                            28.3 (188-190)                
150  H    CH.sub.3                                                        
                ##STR112##                                                
                        H                                                 
                              ##STR113##                                  
                                       CH.sub.3                           
                                          H 44.9; 4.9; 26.2 44.7; 5.0;    
                                            26.3 (222-223)                
151  CH.sub.2 CH.sub.3                                                    
          CH.sub.3                                                        
                ##STR114##                                                
                        CH.sub.2 CH.sub.3                                 
                              ##STR115##                                  
                                       CH.sub.3                           
                                          H 54.7; 6.5; 26.7 54.7; 6.7;    
                                            27.2 (106-107)                
152  CH.sub.2 CH.sub.3                                                    
          CH.sub.3                                                        
                ##STR116##                                                
                        CH.sub.2 CH.sub.3                                 
                              ##STR117##                                  
                                       CH.sub.3                           
                                          H 51.6; 6.1; 25.1 5.15; 6.3;    
                                            25.0 (146-148)                
153  CH.sub.2 CH.sub.3                                                    
          CH.sub.3                                                        
                ##STR118##                                                
                        CH.sub.2 CH.sub.3                                 
                              ##STR119##                                  
                                       CH.sub.3                           
                                          H 48.8; 5.8; 23.7 48.8; 6.0;    
                                            24.3 (183-186)                
154  CH.sub.3                                                             
          H                                                               
                ##STR120##                                                
                        CH.sub.3                                          
                              ##STR121##                                  
                                       H  H 51.0; 5.6; 29.8 51.0; 5.5;    
                                            30.0 (83-85)                  
155  CH.sub.3                                                             
          H                                                               
                ##STR122##                                                
                        CH.sub.3                                          
                              ##STR123##                                  
                                       H  H 47.8; 5.2; 27.9 48.0; 5.3;    
                                            28.0 (150-153)                
156  CH.sub.3                                                             
          H                                                               
                ##STR124##                                                
                        CH.sub.3                                          
                              ##STR125##                                  
                                       H  H 44.9; 4.9; 26.2 45.0; 4.9;    
                                            25.9 (183-185)                
__________________________________________________________________________
EXAMPLE 157 Preparation of 7-Methyl-5-(1-piperazinyl)-1,2,4-triazolo[1,5-c]pyrimidine by Scheme IV
A mixture of 6.00 g (33.3 mmole) of 7-methyl-5-methylthio-1,2,4-triazolo[4,3-c]pyrimidine, 30.0 g (0.35 mmole) of piperazine and 250 ml of dioxane was refluxed under nitrogen for six days. The mixture was cooled and concentrated in vacuo. The residue obtained was dissolved in 150 ml of water, and the solution was extracted four times with 150 ml portions of chloroform. The extracts were washed thrice with 150 ml portions of water and twice with 150 ml portions of sodium chloride solution and were dried over magnesium sulfate. Evaporation provided a yellow solid which was taken up in 150 ml of chloroform, filtered and chromatographed on a high pressure liquid chromatograph, eluting with methanol-ethyl acetate (1:1). Infrared and nuclear magnetic resonance spectral analyses showed fractions 2 and 3 to be 7-methyl-5-(1-piperazino)-1,2,4-triazolo-[1,5-c]pyrimidine, m.p. 92-95 C. Analysis: Calculated for C10 H14 N6 : %C, 55.0; %H, 6.5; %N, 38.5; Found: %C, 54.8; %H, 6.4; %N, 38.3. Fraction 4 contained about 15% of 7-methyl-5-(1-piperazino)-1,2,4-triazolo[4,3-c]pyrimidine.
EXAMPLE 158 Preparation of 7-Methyl-5-(4-methyl-1-piperazinyl)-1,2,4-triazolo[1,5-c]pyrimidine
Using the method of Example 157, 7-methyl-5-methylthio-1,2,4-triazolo[4,3-c]pyrimidine was reacted with N-methylpiperazine to provide a mixture of 7-methyl-5-(4-methyl-1-piperazinyl)-1,2,4-triazolo[4,3-c]pyrimidine, m.p. 170°-171° C. and 7-methyl-5-(4-methyl-1-piperazinyl)-1,2,4-triazolo[1,5-c]pyrimidine, m.p. 95°-98° C. These compounds were separated by high pressure liquid chromatography using 5% methanol in ethyl acetate which contained a small amount of diethylamine.
EXAMPLE 159 Preparation of 7-Methyl-5-(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine
A mixture of 6.0 g (33 mmole) of 7-methyl-5-methylthio-1,2,4-triazolo[4,3-c]pyrimidine and 15 ml of morpholine was heated at reflux for 19 hours, cooled and diluted with diethyl ether and hexane. The solid product was separated by filtration and chromatographed on florisil, eluting sequentially with benzene, 10% ethyl acetate in benzene, 50% ethyl acetate in benzene, and ethyl acetate. Early fractions were recrystallized from a benzene-hexane mixture to provide 7-methyl-5-(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine, m.p. 113°-114° C. Later fractions were recrystallized from an ethyl acetate-hexane mixture with treatment with decolorizing charcoal to provide 7-methyl-5-(4-morpholino)-1,2,4-triazolo[4,3-c]pyrimidine, m.p. 209°-210° C.
EXAMPLE 160
To a solution of 3.00 g (10.8 mmole) of 2,5-diethyl-7-(4-thiomorpholino)-1,2,4-triaziolo[1,5-c]pyrimidine in 60 ml of warm ethanol was added 1.05 g (10.7 mmole) of concentrated sulfuric acid. The solution was diluted to 250 ml with diethyl ether and let stand for two hours. The precipitated product was separated by filtration and washed with ether and dried to provide 2,5-diethyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine dihydrogen sulfate as a white solid, m.p. 178°-180° C. Analysis: Calculated for C13 H19 H5 S.H2 SO4 : %C, 41.5; %H, 5.6; %N, 18.7; Found: %C, 41.7; %H, 5.7; %N, 19.0.
EXAMPLE 161
To a solution of 1.75 g (6.31 mmole) of 2,5-diethyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine in 20 ml of dioxane was added 1.0 ml of 6.4M hydrogen chloride (6.4 mmole) in ethanol. The solution was diluted with 100 ml of diethyl ether and allowed to stand several hours. The white solid was separated by filtration and dried to provide 2,5-diethyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine hydrochloride hydrate, m.p. 113°-114° C. Analysis: Calculated for C13 H19 N5 S.HCl.H2 O: %C, 47.0; %H, 6.7; %N, 21.1; Found: %C, 46.9; %H, 6.8; %N, 21.3.
EXAMPLE 162
To a solution of 2.00 g (7.21 mmole) of 2,5-diethyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine in 20 ml of warm ethanol was added 0.85 g (7.4 mmole) of phosphoric acid. The solution was diluted with 75 ml of diethyl ether and allowed to stand. After a few minutes the white solid was separated by filtration, washed with diethyl ether and dried to provide 2,5-diethyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine trihydrogen phosphate hydrate, m.p. 154°-155° C. Analysis: Calculated for C13 H19 N5 S.H3 PO4.H2 O: %C, 36.4; %H, 6.6; %N, 16.3; Found: %C, 36.5; %H, 5.8; %N, 16.6.

Claims (14)

What is claimed is:
1. A compound of the formula ##STR126## wherein R2 is hydrogen or lower alkyl; at least one of R5 and R7 is ##STR127## wherein each X is independently oxygen, sulfur, sulfinyl, sulfonyl, methylene, imido or lower N-alkylimido; one of R5 and R7 may also be hydrogen or lower alkyl; and R8 is hydrogen, lower alkyl or phenyl; with the proviso that when R5 only is a heterocyclic substituent, X is sulfinyl or sulfonyl; or a pharmaceutically acceptable salt thereof.
2. A compound of the formula ##STR128## wherein R2 is hydrogen or lower alkyl; R7 is ##STR129## wherein X is oxygen, sulfur, sulfinyl, sulfonyl, methylene, imido or lower N-alkylimido; R5 is hydrogen, lower alkyl or ##STR130## wherein X, independently of X in R7, is oxygen, sulfur, sulfinyl, sulfonyl, methylene, imido or lower N-alkylimido; and R8 is hydrogen, lower alkyl or phenyl; or a pharmaceutically acceptable salt thereof.
3. A compound according to claim 1, wherein X is sulfur or oxygen.
4. A compound according to claim 3, wherein R8 is hydrogen.
5. A compound according to claim 2, wherein R5 is hydrogen or lower alkyl.
6. A compound according to claim 2, wherein R8 is hydrogen.
7. A compound according to claim 4, selected from the group consisting of
5,7-bis(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine,
5-(1-morpholino)-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine,
2-ethyl-5-methyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine,
2-ethyl-5-methyl-7-(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine,
2,5-diethyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine,
2-ethyl-7-(4-morpholino)-5-n-propyl-1,2,4-triazolo[1,5-c]pyrimidine,
7-(4-morpholino)-5-n-propyl-1,2,4-triazolo[1,5-c]pyrimidine,
2-ethyl-5-n-propyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine,
5-n-propyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine,
5-ethyl-7-(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine,
5-ethyl-2-methyl-7-(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine, and
2,5-diethyl-7-(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine.
8. The compound 2,5-diethyl-7-(4-thiomorpholino)-1,2,4-triazolo[1,5-c]pyrimidine or a pharmaceutically acceptable salt thereof according to claim 1.
9. The compound 2,5-diethyl-7-(4-morpholino)-1,2,4-triazolo[1,5-c]pyrimidine or a pharmaceutically acceptable salt thereof according to claim 1.
10. A pharmaceutical composition comprising an effective amount of a compound according to claim 1 and a pharmaceutically acceptable vehicle.
11. A bronchodilator pharmaceutical composition, comprising a compound according to claim 2 and a pharmaceutically accetable carrier, wherein said compound is present in an amount which is effective for obtaining bronchodilation.
12. A method for obtaining bronchodilation in a mammal, comprising administering an effective amount of a compound to said mammal, said compound being of the formula ##STR131## wherein R2 is hydrogen or lower alkyl; at least one of R5 and R7 is ##STR132## wherein each X is independently oxygen, sulfur, sulfinyl, sulfonyl, methylene, imido or lower N-alkylimido; one of R5 and R7 may also be hydrogen or lower alkyl; and R8 is hydrogen, lower alkyl or phenyl; or a pharmaceutically acceptable salt thereof.
13. A method for obtaining bronchodilation in a mammal, comprising administering to said mammal a compound according to claim 2 in an amount which is effective for obtaining bronchodilation.
14. A method according to claim 12, wherein the compound is administered orally.
US06/471,836 1983-03-03 1983-03-03 Triazolo[1,5-c]pyrimidines substituted by nitrogen-containing heterocyclic rings Expired - Fee Related US4572910A (en)

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US06/471,836 US4572910A (en) 1983-03-03 1983-03-03 Triazolo[1,5-c]pyrimidines substituted by nitrogen-containing heterocyclic rings
DK135184A DK135184A (en) 1983-03-03 1984-02-29 TRIAZOL (4.3-C) - AND TRIAZOL (1.5-C) PYRIMIDINES
DE8484301383T DE3475193D1 (en) 1983-03-03 1984-03-02 Triazolo(4,3-c)pyrimidines and triazolo(1,5-c)pyrimidines substituted by nitrogen-containing heterocyclic rings
JP59040265A JPS59167592A (en) 1983-03-03 1984-03-02 Triazolo(4,3-c)pyrimidine and triazolo(1,5-c) pyrimidine substituted with nitrogen-containing heterocyclic ring
AT84301383T ATE38670T1 (en) 1983-03-03 1984-03-02 TRIAZOLO(4,3-C>PYRIMIDINE AND TRIAZOLO(1,5-C>PYRIMIDINE) SUBSTITUTED BY NITROGEN-CONTAINING HETEROCYCLIC RINGS.
ZA841603A ZA841603B (en) 1983-03-03 1984-03-02 Triazolo(4,3-c)pyrimidines and triazolo(1,5-c)pyrimidines substituted by nitrogen-containing heterocyclic rings
AU25238/84A AU565545B2 (en) 1983-03-03 1984-03-02 Triazolo(4,3-c) and (1,5-c)pyrimidines
EP84301383A EP0121341B1 (en) 1983-03-03 1984-03-02 Triazolo(4,3-c)pyrimidines and triazolo(1,5-c)pyrimidines substituted by nitrogen-containing heterocyclic rings
IE516/84A IE57026B1 (en) 1983-03-03 1984-03-02 Triazolo(4,3-c)pyrimidines and triazolo(1,5-c)pyrimidines substituted by nitrogen-containing heterocyclic rings
NZ207349A NZ207349A (en) 1983-03-03 1984-03-02 Triazolo(4,3-c)pyrimidines and triazolo(1,5-c)pyrimidines and pharmaceutical compositions
CA000448748A CA1231335A (en) 1983-03-03 1984-03-02 Triazolo 1,5-c pyrimidines substituted by nitrogen-containing heterocyclic rings
KR1019840001076A KR900005836B1 (en) 1983-03-03 1984-03-03 1,2,4-triazolo(4,3-c)pyrimidines
KR1019900009371A KR920000271B1 (en) 1983-03-03 1990-06-25 1,2,4,-triazoro |1.5-c¨ pyrimidine and its preparation process

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US4612375A (en) * 1983-05-02 1986-09-16 Riker Laboratories, Inc. Substituted 4-hydrazino-pyrimides as intermediates for triazolo [4,3-c]pyrimidines
US4734413A (en) * 1987-03-02 1988-03-29 Riker Laboratories, Inc. Substituted 1,2,4-triazolo[1,5-A]triazines as bronchodilators
US4981850A (en) * 1988-02-05 1991-01-01 Riker Laboratories, Inc. Triazolo[1,5-c]pyrimido[1,4]azines as bronchodilators
EP0327284A3 (en) * 1988-02-05 1991-05-02 Riker Laboratories, Inc. Triazolo(1,5-c)pyrimido(1,4) azines as bronchodilators
US5089493A (en) * 1988-02-05 1992-02-18 Riker Laboratories, Inc. Triazolo[1,5-C]pyrimido[1,4]azines as bronchodilators
US5166343A (en) * 1988-02-05 1992-11-24 Riker Laboratories, Inc. Triazolo[1,5-c]pyrimido[1,4]-azines as bronchodilators
US5217973A (en) * 1991-07-05 1993-06-08 Laboratoires Upsa Triazolopyrimidine derivatives which are angiotensin ii receptor antagonists processes for preparing them and pharmaceutical compositions containing them
US5854252A (en) * 1993-03-04 1998-12-29 Shell Internationale Research Maatschappij B.V. Dihalotriazolopyrimidine derivatives
US6222035B1 (en) 1997-03-24 2001-04-24 Kyowa Hakko Kogyo Co., Ltd. [1,2,4] triazolo [1,5-C] pyrimidine derivatives
US6545000B1 (en) 1998-09-22 2003-04-08 Kyowa Hakko Kogyo Co., Ltd. [1,2,4]triazolo[1,5-c]pyrimidine derivatives
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Publication number Priority date Publication date Assignee Title
US4612375A (en) * 1983-05-02 1986-09-16 Riker Laboratories, Inc. Substituted 4-hydrazino-pyrimides as intermediates for triazolo [4,3-c]pyrimidines
US4734413A (en) * 1987-03-02 1988-03-29 Riker Laboratories, Inc. Substituted 1,2,4-triazolo[1,5-A]triazines as bronchodilators
EP0281367A1 (en) * 1987-03-02 1988-09-07 Riker Laboratories, Inc. Substituted 1,2,4-triazolo[1,5-a]triazines as bronchodilators
US4981850A (en) * 1988-02-05 1991-01-01 Riker Laboratories, Inc. Triazolo[1,5-c]pyrimido[1,4]azines as bronchodilators
EP0327284A3 (en) * 1988-02-05 1991-05-02 Riker Laboratories, Inc. Triazolo(1,5-c)pyrimido(1,4) azines as bronchodilators
US5089493A (en) * 1988-02-05 1992-02-18 Riker Laboratories, Inc. Triazolo[1,5-C]pyrimido[1,4]azines as bronchodilators
US5166343A (en) * 1988-02-05 1992-11-24 Riker Laboratories, Inc. Triazolo[1,5-c]pyrimido[1,4]-azines as bronchodilators
US5217973A (en) * 1991-07-05 1993-06-08 Laboratoires Upsa Triazolopyrimidine derivatives which are angiotensin ii receptor antagonists processes for preparing them and pharmaceutical compositions containing them
US5854252A (en) * 1993-03-04 1998-12-29 Shell Internationale Research Maatschappij B.V. Dihalotriazolopyrimidine derivatives
US6222035B1 (en) 1997-03-24 2001-04-24 Kyowa Hakko Kogyo Co., Ltd. [1,2,4] triazolo [1,5-C] pyrimidine derivatives
US6545000B1 (en) 1998-09-22 2003-04-08 Kyowa Hakko Kogyo Co., Ltd. [1,2,4]triazolo[1,5-c]pyrimidine derivatives
US20060058320A1 (en) * 2002-09-24 2006-03-16 Kyowa Hakko Kogyo Co., Ltd. [1,2,4] Triazolo[1,5-c]pyrimidine derivatives
CN113692306A (en) * 2019-04-03 2021-11-23 泰拉强石株式会社 Triazolopyrimidines based on thymine nucleobases and process for their preparation
US12351583B2 (en) 2019-04-03 2025-07-08 Chemiteras, Inc. Triazolopyrimidines based on thymine nucleobase and methods for producing them

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