US3925207A - Semi-automatic chromatographic separation apparatus - Google Patents

Semi-automatic chromatographic separation apparatus Download PDF

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Publication number
US3925207A
US3925207A US564465A US56446575A US3925207A US 3925207 A US3925207 A US 3925207A US 564465 A US564465 A US 564465A US 56446575 A US56446575 A US 56446575A US 3925207 A US3925207 A US 3925207A
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United States
Prior art keywords
carrier
flasks
carrier arm
chromatographic columns
carrier plate
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Expired - Lifetime
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US564465A
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English (en)
Inventor
Peter C Scriba
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sartorius Membranfilter GmbH
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Sartorius Membranfilter GmbH
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/466—Flow patterns using more than one column with separation columns in parallel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/04—Preparation or injection of sample to be analysed
    • G01N30/24—Automatic injection systems

Definitions

  • the invention relates to an apparatus for the simultaneous semi-automatic column chromatographic separation of n sample solutions which are each separated into at least two fractions in the column with the aid of wash and/or buffer solutions.
  • the apparatus comprises a proportionating pump with n channels, which are each connected upstream to a suction needle and downstream to a column of a group of n chromato graphic columns.
  • a fraction collector comprising n collection flasks is arranged beneath the group of columns.
  • the invention relates to an apparatus for the simultaneous semi-automatic column chromatographic separation of :1 sample solutions which are each separated into at least two fractions in the column with the aid of wash and/or buffer solutions.
  • the apparatus comprises a proportionating pump with n channels, which are each connected upstream to a suction needle and downstream to a column of a group of n chromatographic columns.
  • a fraction collector comprising n collection flasks is arranged beneath the group of columns.
  • an apparatus of the above type is provided by a carrier arm carrying the n suction needles, which is displaceable relative to a surface for supporting supply flasks for the solutions, not only in the axial direction of the suction needles but also at right angles thereto into predetermined positions, a carrier plate on which collection flasks can be placed and which is displaceable under the column group at right angles to the axis of the columns into predetermined working positions for the collection flasks, and a time control arrangement with adjustable time elements which co-ordinates the displacements of the carrier arm, the pump and the carrier plate.
  • a significant advantage of the invention consists in that the apparatus, once the sample solutions as well as the wash and buffer solutions have been prepared and arranged in their provided positions on the carrier surfaces and the apparatus set in action, the column chromatographic separation proceeds automatically without the work of personnel being required.
  • a further advantage of the invention consists in that a large number of sample solutions, namely 20 or 30 sample solutions and more, can be chromatographically separated at the same time.
  • an advantage of the invention consists in that the same person who set the apparatus into operation can already examine the first fractions obtained by the process while the remaining fractions are still being produced on their own by the apparatus. This is particularly meaningful in separation by column chromatography of sample solutions in which the measurable radioactivity of individual fractions is important and the half-life period of the active elements is short. In this case the measurement of the radioactivity can be begun with the first fraction as soon as this has been fully produced and production of the next has been commenced.
  • the carrier arm is preferably slideable along its length, whilst the carrier arm and/or the carrier surface is adjustable in height.
  • the carrier arm is rotatable and the turning axis of the carrier arm is adjustable in height whilst the drive for the carrier arm is advantageously a motor which can be arrested in predetermined positions by brakes.
  • this motor is a step-switch motor whilst in another preferred improvement of the invention the motor is an 2 asynchromatic motor and the brake is a DC. current brake.
  • the drive for the carrier ann advantageously comprises a contact which is displaced with displacement of the carrier ann, which is alloted to adjustable stationary contacts by means of which the predetermined drive positions of the carrier arm can be set.
  • the movable contact is arranged on a ring, whilst the stationary contacts are arranged outwardly of the ring about its circumference.
  • a rest is provided for the carrier arm for each position into which it may be driven.
  • the sample flasks with the different solutions can be arranged in predetennined positions on the carrier surface.
  • the carrier arm is automatically elevated by at least the length of the suction needles and is turned into the necessary position in which it is again lowered and the suction needles carried on the arm dip into the sample flask standing in that position.
  • the arm is again elevated, turned into the next predetermined position and lowered in that position.
  • the suction needles are adjustable in height.
  • the carrier arm has openings for receiving the suction needles, whereby each suction needle is removable from its particular opening and is advantageously flexibly located.
  • supply containers for the wash and buffer solutions are arranged relative to the carrier surface for the sample flasks so that each flask is connectible with the particular sample flask in accordance with the syphon principle.
  • the proportionating pump is preferably a tube pump which advantageously comprises at least 25 channels. In this manner it is possible to process just as high a number of different samples at the same time.
  • each collector device carrying the collection flasks comprises at least one member which couples with a complementary member on the carrier plate when placing the collector device and which fixes the situation of the collector device for this particular predetermined position on the carrier plate.
  • each collector device advantageously comprises one carrier tube at each corner which are, apart from one, all blocked to provide a carrier surface, whilst the carrier plate in the predetermined positions for the collector device which is provided with insert openings for its collector tubes, is provided with a protruding pin to fit into the insert opening formed by the open bore.
  • each collector device can only be arranged in a very particular manner, namely in such a manner that the protruding pin is inserted into the op en bore.
  • each collector device can only be arranged in a very particular manner, namely in such a manner that the protruding pin is inserted into the op en bore.
  • each collector device for the collection flasks may be placed in one quadrant of the carrier plate.
  • a disc is connected to the turning axis of the turntable, which provides four rest positions displaced at while this disc is provided with at least one pivotally arranged rest element which is biased by a spring with a rest nose against the circumference of the disc.
  • two opposing pivotable rest elements are provided in which the rest noses are biased against the periphery of the disc by a common spring.
  • a conventional spring arrangement is provided; however the rest elements with their rest noses could be lifted out of the rest positions of the disc by electromagnets.
  • the chromatographic columns are arranged in a common block, which is preferably a block which may be kept at the same temperature by means of a thermostat.
  • a closure valve is arranged on the bottom of the block, which is adjustable between an open position in which the outlet openings of the columns are open and a closed position in which the outlet openings are closed.
  • the closure valve is a plate which is provided with a number of silicone seals corresponding to the number of the chromatographic columns.
  • the proportionating pump is preferably reversible in its direction of rotation.
  • the proportionating pump can then at the beginning of the position changes of the carrier plate for the collector devices be reversed for a short time to suck in the drops suspended at the openings of the chromatographic columns and thus prevent a possible mixing of the individual fractions.
  • the timing elements of the time control arrangement can be switched off during this position change.
  • the proportionating pump, the fraction collector and the carrier arm form a common housing by which the carrier surfaces are presented and within which the drive for the carrier arm and the carrier plate is located.
  • the housing forms a pedestal in its central portion for carrying the proportionating pump, to one side of which pedestal is a step forming the carrier surface for the sample flasks and on the opposite side of the pedestal a lower set plateau for the fraction collector, a closure plate extending from the pedestal of the housing on the side opposite to the carrier surface being provided.
  • This embodiment is compact and can in relation to its weight of about 60 kg, without pump and without control portion, be relatively easily transported and rearranged.
  • the timing elements of the time control arrangement are advantageously clocks which can be set between the time zero and a maximum time.
  • the chromatographic system and the time control arrangement are contained in separate housings which are electrically connected to one another by a cable.
  • time control arrangement makes a very broad pre-selection possible for the timing of individual time steps of an operation, whilst it is at the same time possible after the selection of the particular time step eliminations, to eliminate such by setting the provided clock at zero.
  • an apparatus can be advantageously provided in which a substitute block with chromatographic columns can be connected up.
  • the apparatus in accordance with the invention is advantageously employed in the hormone analysis of medical solutions and preferably in the analysis of the thyroid gland hormone Tri-iodide Thyronin (T and Thyroxin (T).
  • T and Thyroxin T
  • the invention is described by way of example below with reference to the drawings; in these there is schematically shown:
  • FIGS. 1 and 2 a side view and plan view respectively of an apparatus for column chromatographic separation of sample solutions, which serve to explain the process,
  • FIG. 3 a side view of a practical embodiment of an apparatus in accordance with the invention for semiautomatic column chromatographic separation
  • FIG. 4 a side view of the carrier arm, in which this is in section in the area of a suction needle to expose the spring mounting of the suction needle.
  • a supply flask l for a buffer solution and an incubation flask 8 (FIG. 2) with inserted test tubes 2 for taking up of the sample solutions is arranged upstream from a roller pump 10.
  • a suction needle 11 is shown dipped into the test tube 2 shown in FIG. 1, which is arranged on the end of a tube 9 of a group of tubes which is upstream of the roller pump 10.
  • a thermostatically controllable block 5, with inserted chromatographic columns 3, is arranged downstream from the roller pump 10.
  • the columns 3 are arranged vertically in the block 5 although for clarity they are shown in the plane of the drawing in FIG. 2.
  • a closure lid 4 is arranged at the top end of the block 5 which extends over the total breadth and length of the block.
  • Each column 3 is connected to the roller pump 10 through the closure lid 4 by the downstream end of a tube 9.
  • the columns 3 are Sephadex columns which contain Dextran gel.
  • the drain 7 is provided for collection of the buffer solution which is then pumped through the Sephadex columns, which is disposed of as rinse solution.
  • a housing 46 which forms an elevated platform 48 in its middle portion, on which a roller pump 38 is arranged.
  • the housing 46 forms a step whose surface provides carrier surface 26 on which the supply flasks 28 for the samples, wash and buffer solutions can be placed.
  • a carrier arm 24 is connected to the housing 46 on the upstream side of the roller pump 38 and has a turning axis 40 which carries the carrier arm 24 at a predetermined distance above the carrier surface 26.
  • Suction needles 22 are inserted in the carrier arm 24 which, in the representation of FIG. 3 are dipped into the supply flasks 28, whereby the suction needles, which are advantageously spring mounted, are displaced upwardly l to 2 millimeters relative to the carrier arm when pressed onto the bottom of the supply flasks 28. It is thus achieved that the sample solution is completely sucked off.
  • the carrier arm 24 with its turning axle 40 is adjustable in height relative to the carrier surface 26 and is tumable about the turning axle 40 into predetermined angular positions.
  • the setting of the height is obtained with a lifter, which is at least as long as the suction needles 22 so that their bottom ends in the top position of the lifter of the carrier arm are out of the supply flasks 28 or out of such a flask in which it was located.
  • the lifter is slightly larger than the height of supply flasks 28.
  • the motors required for the height adjustment and turning of the carrier arm 24 are arranged in the housing 46 beneath the carrier surface 26.
  • the predetermined angular positions of the carrier arm 24 are clearly marked on the carrier surface 26 of the housing 46 so that the supply flasks 28 and the tubes with the sample solution containers can be placed exactly into their provided positions in only one direction on'the carrier surface 26, whereby the supply flask for the sample solution can only be arranged in one predetermined position.
  • Each suction needle 22 is connected at its top end to the upstream end of a tube 42 of the roller pump 38.
  • a thermostatic controllable block 36 containing chromatographic columns and a fraction collector 44.
  • the block 36 stands on a carrier plate 50 which extends from the pedestal 48 of the housing 46 on the side opposite to the carrier surface 26.
  • the top end of each column is connected to the downstream end of a tube 42 of the roller pump 38.
  • the fraction collector 44 is so arranged beneath the carrier plate 50 that there is still space between its upper end and the underside of the carrier plate 50.
  • the fraction collector 44 comprises a carrier plate 30 which is connected to a turning axle 54 which is located in the housing 46 beneath the plateau 52.
  • the fraction collector 44 comprises carrier devices 32 into which the collector flasks 34 for the fractions may be inserted. Carrier devices for two types of collection flasks are thus contemplated.
  • the carrier plate 30 of the fraction collector 44 is made in the form of a turntable tumable on its turning axle 54 by means of a motor arranged in the housing 46.
  • the carrier plate 30 is formed approximately quadratically in the same way as the carrier device 32, whereby one carrier device 32 can always be placed on one quadrant of the carrier plate 30.
  • the carrier plate 30 may be arrested in four displaced positions at 90 relative to one another, whereby a carrier device 32 is arranged exactly beneath the thermostatically controllable block 36 and a collection flask 34 in this carrier device 32 is beneath each lower end of a column in each of these four positions.
  • the motors for setting the height of the axle 40 of the carrier arm 24, for the turning of the carrier arm 24, for the drive of the roller pump 38 and for the turning of the fraction collector 44 are co-ordinatively controlled by a common time controlled arrangement.
  • the time control arrangement has adjustable time elements to each of which a time can be allocated. Both the portion of the apparatus which is arranged upstream of the pump and that portion which is arranged downstream is allocated to its own group of timing elements which proceed one after the other, whereby the time elements allocated to the upstream group and the time elements allocated to the downstream group can function independently of one another.
  • the time elements of one group can be connected one after the other so that the next following time element is set into operation only after the time has passed which was set for a time element. It is thus possible to pre-select at will the time for the individual steps in carrying out the semi-automatic column chromatographic separation.
  • a plate 55 is arranged at the bottom of the block 36 as closure valve, which has as many silicone seals as chromatographic columns contained in the block 36.
  • the drive of the plate is effected over a member which is mounted on a rotatable eccentric.
  • the eccentric is secured to an axis which extends from the housing 46 and is provided with a control knob. By turning the knob the plate 55 is displaced and the chromatographic columns are thus closed or opened.
  • the suction needles 22 in the carrier arm 24 are spring supported. Each suction needle 22 is inserted for this purpose in a sheath 56.
  • the sheath 56 forms a broadened annular flange 58 which is slidably displaceable in the bore 60 provided in the carrier arm for the suction needle 22.
  • a compression spring 62 is arranged in the bore 60 which on the one hand presses against the annular flange 58 of the sheath 56 and on the other hand against the annular shoulder 64 provided in the bore 60.
  • the annular shoulder 64 is situated at the top end of the bore 60 in the upper end position of the carrier arm 24, whilst in the rest position of the suction needle 22, the annular flange 58 is situated at the bottom end of the bore 60 with its bottom surface about level with the bottom side of the carrier arm 24.
  • suitable security can be provided which prevents that the sheath 56 of the suction needle 22 falls downwardly out of the carrier arm 24. This can for example be achieved by a plate with openings whose diameter is smaller than the outer diameter of the annular flanges 58 of the sheath 56.
  • the compression springs 62 arranged in the bores 60 act on the sheaths 56 and thus the suction needles 22 are in their rest position.
  • the suction needles 22 are displaced upwardly with further lowering of the carrier arm against the action of the compression spring 62.
  • the carrier arm 24 is in operation lowered so far that each suction needle is displaced l to 2 millimeters against the action of its allocated compression spring 62. It is thus ensured that the sample solution in the flask is completely sucked out.
  • An apparatus for the simultaneous semi-automatic chromatographic separation of n sample solutions into a least two fractions which comprises n chromatographic columns, n flexible tubes each connected at one end to one inlet of the n chromatographic columns and connected at the other end to a hollow suction needle, a roller pump arranged to act on the n flexible tubes and thus pump sample solutions into which the suction needles may be dipped to the chromatographic columns, a collector device comprising n fraction collection flasks for each fraction, a carrier arm in which the n suction needles are mounted in parallel spaced relationship with their free ends extending from the carrier arm, the carrier arm being displaceable both in the direction of the length-wise axes of the needles as well as at right angles thereto into predetermined positions relative to a carrier surface for sample solution flasks, a carrier plate situated beneath the outlets of the chromatographic columns and onto which collector devices for carrying fraction collection flasks may be placed in predetermined positions, the carrier plate being rotatable in a plane
  • suction needles are displaceable along their axes relative to the carrier arm, against a spring action acting between the carrier arm and the suction needles.
  • each collector device for carrying the fraction collection flasks is provided with at least one element which fits with a complimentary element provided on the carrier plate and thus fixes the collector device in its predetermined position on the carrier plate.
  • An apparatus in which there is provided a common housing for housing drive means for driving the carrier arm and carrier plate, and in which a surface of said housing forms the carrier surface for carrying sample solution flasks.
  • the housing is shaped to form a central pedestral for carrying the roller pump, the carrier surface for carrying sample solution flasks then being to one side of the platform and a deeper set plateau being provided to the other side of the platform over which the carrier plate is mounted, and in which a closure plate is provided for closing off access to fraction collector flasks, the closure plate extending sidewardly from the pedestal on the side of the plateau.
  • An apparatus in accordance with claim 1 further including a column block in which said n chromatographic columns are disposed.

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Sampling And Sample Adjustment (AREA)
US564465A 1974-04-17 1975-04-02 Semi-automatic chromatographic separation apparatus Expired - Lifetime US3925207A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE2418509A DE2418509C3 (de) 1974-04-17 1974-04-17 Vorrichtung zur halbautomatischen gleichzeitigen säulenchromatographischen Auftrennung einer Vielzahl von Probenlösungen

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US3925207A true US3925207A (en) 1975-12-09

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US564465A Expired - Lifetime US3925207A (en) 1974-04-17 1975-04-02 Semi-automatic chromatographic separation apparatus

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US (1) US3925207A (enrdf_load_stackoverflow)
JP (1) JPS512489A (enrdf_load_stackoverflow)
CH (1) CH596868A5 (enrdf_load_stackoverflow)
DE (1) DE2418509C3 (enrdf_load_stackoverflow)
FR (1) FR2268267B3 (enrdf_load_stackoverflow)
GB (1) GB1470276A (enrdf_load_stackoverflow)

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4228008A (en) * 1979-04-05 1980-10-14 Hoffmann-La Roche Inc. Chromatographic device for simultaneous collection and evaporation of sequential volatile non-aqueous eluates
US4422941A (en) * 1980-09-08 1983-12-27 University Of Pittsburgh Apparatus for liquid-solid column centrifugation chromatography and method
US4806250A (en) * 1985-12-12 1989-02-21 Hitachi, Ltd. Liquid chromatography and apparatus using the same
US4805469A (en) * 1986-06-17 1989-02-21 Rhone-Poulenc Recherches Apparatus for automatically taking and analyzing samples of substances which are made available in random manner
US5091092A (en) * 1989-05-03 1992-02-25 Analytical Bio-Chemistry Laboratories, Inc. Single-loop chromatography system and method
US5107908A (en) * 1989-05-03 1992-04-28 Analytical Bio-Chemistry Laboratories, Inc. Apparatus for supporting a container for fluid material
US5207918A (en) * 1989-10-27 1993-05-04 Helena Laboratories Corporation Column analyzer system
EP0425297A3 (en) * 1989-10-27 1993-05-05 Helena Laboratories Corporation Column analyzer system
US5228988A (en) * 1989-10-27 1993-07-20 Helena Laboratories Corporation Column analyzer system and improved chromatograph column for use in the system
FR2705582A1 (fr) * 1993-05-25 1994-12-02 Commissariat Energie Atomique Dispositif automatique de traitement par chromatographie sur colonne et détecteur de liquide utilisable dans un tel dispositif.
US5395521A (en) * 1991-05-31 1995-03-07 Board Of Regents, The University Of Texas System Automated column equilibration, column loading, column washing and column elution
US5443734A (en) * 1990-03-05 1995-08-22 Applied Separations, Inc. Programmable solid phase extraction and elution device
US6652746B2 (en) * 2002-03-26 2003-11-25 Biotage, Inc. Chromatography system for automatically separating different compounds in a sample
US20040040850A1 (en) * 1997-10-24 2004-03-04 Northeastern University Multichannel microscale system for high throughput preparative separation with comprehensive collection and analysis
US20050224402A1 (en) * 2004-03-03 2005-10-13 Bionisis Installation for separating components in a plurality of parallel channels
US20120048734A1 (en) * 2010-08-25 2012-03-01 Arkray, Inc. Analysis Apparatus and Analysis Method

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0658400B2 (ja) * 1987-03-04 1994-08-03 株式会社巴川製紙所 放射線検知組成物および放射線線量測定用シ−ト
DE19704477A1 (de) * 1997-02-06 1998-08-13 Solvay Pharm Gmbh Vorrichtung und Verfahren zur Parallel-Chromatographie
RU2185871C1 (ru) * 2001-01-09 2002-07-27 Санкт-Петербургская общественная организация "Невская академия изобретателей" Препаративный хроматограф

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3156548A (en) * 1960-03-21 1964-11-10 Sinclair Research Inc Gas chromatography apparatus
US3504799A (en) * 1968-04-02 1970-04-07 Beckman Instruments Inc Sample injector
US3649203A (en) * 1968-11-22 1972-03-14 Ralston Purina Co Automatic analyzer
US3701609A (en) * 1971-05-13 1972-10-31 David G Bailey Apparatus for automatically adding preselected patterns of eluent solutions to a chromatographic column and monitoring and collecting eluted fractions

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3156548A (en) * 1960-03-21 1964-11-10 Sinclair Research Inc Gas chromatography apparatus
US3504799A (en) * 1968-04-02 1970-04-07 Beckman Instruments Inc Sample injector
US3649203A (en) * 1968-11-22 1972-03-14 Ralston Purina Co Automatic analyzer
US3701609A (en) * 1971-05-13 1972-10-31 David G Bailey Apparatus for automatically adding preselected patterns of eluent solutions to a chromatographic column and monitoring and collecting eluted fractions

Cited By (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4228008A (en) * 1979-04-05 1980-10-14 Hoffmann-La Roche Inc. Chromatographic device for simultaneous collection and evaporation of sequential volatile non-aqueous eluates
US4422941A (en) * 1980-09-08 1983-12-27 University Of Pittsburgh Apparatus for liquid-solid column centrifugation chromatography and method
US4806250A (en) * 1985-12-12 1989-02-21 Hitachi, Ltd. Liquid chromatography and apparatus using the same
US4805469A (en) * 1986-06-17 1989-02-21 Rhone-Poulenc Recherches Apparatus for automatically taking and analyzing samples of substances which are made available in random manner
US5091092A (en) * 1989-05-03 1992-02-25 Analytical Bio-Chemistry Laboratories, Inc. Single-loop chromatography system and method
US5107908A (en) * 1989-05-03 1992-04-28 Analytical Bio-Chemistry Laboratories, Inc. Apparatus for supporting a container for fluid material
US5441645A (en) * 1989-10-27 1995-08-15 Helena Laboratories Corporation Column analyzer system and improved chromatograph column for use in the system
US5589063A (en) * 1989-10-27 1996-12-31 Helena Laboratories Corporation Column analyzer system and improved chromatograph column for use in the system
US5228988A (en) * 1989-10-27 1993-07-20 Helena Laboratories Corporation Column analyzer system and improved chromatograph column for use in the system
US5358641A (en) * 1989-10-27 1994-10-25 Helena Laboratories Corporation Column analyzer system and improved chromatograph column for use in the system
US5595664A (en) * 1989-10-27 1997-01-21 Helena Laboratories Corporation Column analyzer system and improved chromatograph column for use in the system
EP0425297A3 (en) * 1989-10-27 1993-05-05 Helena Laboratories Corporation Column analyzer system
US5207918A (en) * 1989-10-27 1993-05-04 Helena Laboratories Corporation Column analyzer system
US5443734A (en) * 1990-03-05 1995-08-22 Applied Separations, Inc. Programmable solid phase extraction and elution device
US5512168A (en) * 1990-03-05 1996-04-30 Applied Separations, Inc. Programmable solid phase extraction and elution device
US5395521A (en) * 1991-05-31 1995-03-07 Board Of Regents, The University Of Texas System Automated column equilibration, column loading, column washing and column elution
FR2705582A1 (fr) * 1993-05-25 1994-12-02 Commissariat Energie Atomique Dispositif automatique de traitement par chromatographie sur colonne et détecteur de liquide utilisable dans un tel dispositif.
US20040040850A1 (en) * 1997-10-24 2004-03-04 Northeastern University Multichannel microscale system for high throughput preparative separation with comprehensive collection and analysis
US20040040851A1 (en) * 1997-10-24 2004-03-04 Northeastern University Multichannel microscale system for high throughput preparative separation with comprehensive collection and analysis
EP1025434A4 (en) * 1997-10-24 2006-10-18 Univ Northeastern MULTI-CHANNEL PREPARATIVE SEPARATION MICROSYSTEM WITH HIGH PERFORMANCE COLLECTION AND EXHAUSTIVE ANALYSIS
US7578915B2 (en) 1997-10-24 2009-08-25 Northeastern University Multichannel microscale system for high throughput preparative separation with comprehensive collection and analysis
US6652746B2 (en) * 2002-03-26 2003-11-25 Biotage, Inc. Chromatography system for automatically separating different compounds in a sample
US20030222004A1 (en) * 2002-03-26 2003-12-04 Biotage, Inc. Chromatography system for automatically separating different compounds in a sample
US20050224402A1 (en) * 2004-03-03 2005-10-13 Bionisis Installation for separating components in a plurality of parallel channels
US20120048734A1 (en) * 2010-08-25 2012-03-01 Arkray, Inc. Analysis Apparatus and Analysis Method
US8758587B2 (en) * 2010-08-25 2014-06-24 Arkray, Inc. Analysis apparatus and analysis method

Also Published As

Publication number Publication date
DE2418509C3 (de) 1978-04-27
FR2268267B3 (enrdf_load_stackoverflow) 1978-10-06
GB1470276A (en) 1977-04-14
DE2418509A1 (de) 1975-10-30
FR2268267A1 (enrdf_load_stackoverflow) 1975-11-14
DE2418509B2 (de) 1977-08-25
JPS512489A (en) 1976-01-10
CH596868A5 (enrdf_load_stackoverflow) 1978-03-31

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