US3909419A - Plasma separator with squeezed sealant - Google Patents

Plasma separator with squeezed sealant Download PDF

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Publication number
US3909419A
US3909419A US44636774A US3909419A US 3909419 A US3909419 A US 3909419A US 44636774 A US44636774 A US 44636774A US 3909419 A US3909419 A US 3909419A
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Prior art keywords
blood
phase
cylinders
container
heavy
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Expired - Lifetime
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Waldemar A Ayres
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Becton Dickinson and Co
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Becton Dickinson and Co
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5021Test tubes specially adapted for centrifugation purposes
    • B01L3/50215Test tubes specially adapted for centrifugation purposes using a float to separate phases

Abstract

A blood collection and separator assembly of the type suitable for use in centrifuging blood to separate the plasma, the light phase, from the cellular portion, the heavy phase. The assembly includes a collection container and a pair of cylinders slidably disposed in the container. The first or top cylinder has a specific gravity greater than the light phase and the second or bottom cylinder has a specific gravity lighter than the heavy phase. Interposed between the cylinders are one or more layers of micro encapsulated beads of gelatin or other sealant. The average specific gravity of the first plus the second cylinders is between that of the plasma and red cells, so that the pair of cylinders float on and above the top of the heavy phase, thereby establishing the seal slightly above the interface between the light phase and heavy phase of the blood. When centrifuged the two cylinders move to the top of the packed red cells and break and squeeze out the sealant thereby forming a seal between the cylinders and the inner wall of the container slightly above the interface between the two phases of the blood.

Description

9 Umte States Patent 1 1 1 1 3,909,419

Ayres Sept. 30, 1975 PLASMA SEPARATOR WITH SQUEEZED [57] ABSTRACT SEALANT Inventor: Waldemar y s Rutherford A blood collection and separator assembly of the type suitable for use in centrifuging blood to separate the [73] AssigneeI Becton, Dickinson and Company, 1 plasma, the light phase, from the cellular portion, the

East Rutherford, NJ heavy phase. The assembly includes a collection container and a pair of cylinders slidably disposed in the Filedi 27, 1974 container. The first or top cylinder has a specific grav- [21] Appl. No.: 446,367 ity greater than the light phase and the second or bottom cylinder has a spec1fic gravity lighter than the 1 heavy phase. Interposed between the cylinders are one U.S-

or morg layers of micro encapsulated beads of gelatin [51] Int. Cl? BOlD 21/26 or other l t, The average specific gravity of the Field of Search 23/230 13, 258-5, 292; first plus the second cylinders is between that of the 128/2 F, 214 R, 218 M, 272; 210/83, 8 plasma and red cells, so that the pair of cylinders float 109, 131, 359, 5 A, 518, 1316- 23, 24; on and above the top of the heavy phase, thereby es- 233/1 A, 1 26 tablishing the seal slightly above the interface between the light phase and heavy phase of the blood. When l l References Cited centrifuged the two cylinders move to the top of the UNITED STATES PATENTS packed red cells and break and squeeze out the seal- 3,437,266 4/1969 Patterson .2 210/010. 23 am thereby forming a Seal between the Cylinders and 3,647,070 3/1972 Adler 210/83 h inn r ll f h n ine g ly above h in r- 3 780 935 12/1973 Lukacs et al. ZlO/DIG. 23 face between the two phases of the blood.

5 Claims, 2 Drawing Figures PLASMASEPARAT OR WITH SQUEEZED I SEALANT w I BACKGROUND OF'THE INVENTION" 1 It is known to separate blood intolits cornponlent parts by centrifugation, for example, theassembly-disclosed in- US. Pat. No; 2,460,641.- However, this particular assembly'does'not employ afmeans forsealing the separated plasma or serum phase""fror'nthe cellular phase? It is also known to provide assemblies for 'imanually separating the plasma ,orserum phase frc pr'ri th e cellular phase for example, as disclosed in U.S P at. Nos. 3,586,064; 3,661,265; 7 3,355,098; -.3,481,477; 3,512,940; a i3,693,s04. In all of thesedevices the serumiscollectd in ablood collection container and means are provided for separating the plasma 'orser'um phase from the cellular pha'se employing filters, valves, transfer tubes or. the like. I I I It is also known to provide assemblies for thesealed separation of, blood in which a piston isactuated by centrifugalforce such as is disclosed in PaLNos. 3,508,653 and 3,779,383. These devices use either a distortable piston made of resilient material or valve means associated with the piston toeffect a sealed separation after centrifugation. It is also known that a liquid sealant can be used for the separated portions of a blood sample as is coveredby US. Pat. No 3,780,935, but such patent does not disclose the two, cylinders for squeezing out the sealant of the present invention.

SUM ARY OF THEmvENTIO'N I It is an object of the present invention ito provide a plasma separator assembly including a collection container and a pair ofcylinders with encapsulated sealant interposed between the cylinders slidably disposed in the container, which cylinders havespecifi'c gravities respectively greater and lower than blood s o that upon centrifugation the sealant between the cylinders will be released and form a seal between the cylinders and the container wall slightly above the plasma-cellular interface.

It is another object of the invention to provide a plasma separator assembly which is economical to manufacture and can be used in conjunction with standard blood collecting equipment.

DESCRIPTION OF THE DRAWINGS For a better understanding of the invention, references are made to the drawings which illustrate the preferred embodiment of the invention herein.

FIG. 1 is a sectional, elevational view showing the plasma separator assembly of the present invention and also illustrating a pointed cannula penetrating one of .the stoppered ends of the container through which blood is introduced into the container prior to its separation.

FIG. 2 is a sectional, elevational view similar to the view of FIG. 1, showing the two cylinders and the interposed sealant forming an impervious barrier across the container at the plasma-cellular interface.

. IDES RIPUON OFTI-IELPREFERIRED EMBODIMENT For a better ilnderstanding of the invention herein a descriptioh of thedrawing s of the illustrative embodiishad'with particular reference to FIGS. 1 and i nula 14 so that-blood canb e transferred from a blood source'into the container under aseptic conditions'The closures 12and'13 are preferably self-sealing so that when the cannula is removed from the closure 12 there will be no loss of blood passing through 'the penetration portion of closure 12 illustrated in FIG." 1.

Disposed in the container area pair of sliding cylinde'rs, the first or top cyIin'der IS'having a specific grav it'yjgreater thanthe light phase of blood (greaterth ani 1.03) andthe second or bottom cylinder 16 having specific gravity lower than the heavy phase of blood (less than 1.09 sp.g. The average specific gravity of the first cylinder plus the second cylinder is between the plasma and red cells, for example 1.06. The fir lst cylinder carries one or more layers of rnicro encapsulated beads 17 abstaining gelatin or other sealant 18.

,When operating the separator assembly of the invention herein it is preferred that the assembly be evacuated sothat when cannula l4 penetrates closure 12 blood will fillcontainer l1 automatically. It is also contemplated to provide a separator, assemblysuitablefor use with blood collecting assembly disclosediin US. Pat, Nos. 2,460,641, 3,469,572 and 3,494,352.

After,the cannula14 is withdrawn and container 11 is filled-withbloodtheassernbly is placed ina centrifuge and the blood is separated by initially employing moderate centrifugal force. This precipitates or separates theblood cells into the lower portion of container 11 and automatically moves the cylinders .15 and 16 to float on the plasma-red cell interface. Thereafter the rotational speed of the centrifuge is increased which causes the squeeze between the cylinders to increase and ruptures the encapsulated beads and squeezes out the sealant (FIG. 2). The sealant surrounds the two cylinders and contacts the inner wall of the container and forms a seal across the container between the light and heavy phases of the blood.

Then the centrifugal forces are terminated and the separated blood sample is ready for use. As desired, the

plasma can be taken from one end and/or the concentrated red cells can be taken from the other end.

While variations of the invention herein may be had, the objectives of the invention have been illustrated and described.

Having described the invention, what is claimed is:

1. A separator assembly, capable of separating blood into its component light phase or plasma and heavy phase or cellular portion comprising:

a. a container having at least one open end which is adapted to receive blood for subsequent separation into a light phase and a heavy phase;

b. a closure sealing the open end of the container, the closure being formed of a self-sealing elastomeric material which is penetrable by a cannula through which blood to be separated is conducted into the container;

c. a pair of cylinders and rupturable capsules of sealant slidably mounted in the container, the upper cylinder having a specific gravity greater than the light phase, the lower cylinder having a specific gravity less than the heavy phase, and the combined cylinders plus capsules having a specific gravity between the two phases of blood; and

d. said rupturable capsules of sealant having a specific gravity between the two phases of blood and being interposed between the two cylinders, whereby when centrifugal force is used the blood separates into its light and heavy phases, the cylinders move through the light phase and come to a 7 stop at the top of the heavy phase, and the pressure between the cylinders ruptures the capsules and releases the sealant to form a seal slightly above the interface between the phases.

2. The separator of claim 1 wherein the said container comprises a tubular body open at each end in which closures formed of elastomeric material are mounted in sealing engagement with the tubular body and the cylinders are disposed adjacent one of said closures.

3. The separator of claim 1 wherein the sealant is gelatin.

4. A separator assembly capable of separating blood into its component light phase, plasma, and heavy phase, cellular portion comprising:

a. a container for receiving blood having closures at each end for sealing the container;

b. a pair of cylinders plus rupturable capsules of sealant slidably mounted in the container adjacent the upper closure, the upper cylinder having a specific gravity greater than the light phase, the lower cylinder having a specific gravity less than the heavy phase of blood, and the combined cylinders plus capsules having a specific gravity between the two phases of blood; and

c. said rupturable capsules of sealant having a specific gravity between the two phases of blood and being interposed between the two cylinders,

whereby when centrifugal force is used the blood separates into its light and heavy phases, the cylinders move through the light phase and come to a stop at the top of the heavy phase, and the pressure between the cylinders ruptures the capsules and releases the sealant to form a seal slightly above the interface between the phases. 5. A separator assembly capable of separating blood into its component light phase of plasma and heavy 10 phase of cellular portion comprising:

a. a tubular container having two open ends;

b. resilient closure means adapted to close said ends with at least one said closure means capable of being penetrated by a cannula for filling with blood;

c. a slidable pair of cylinders plus rupturable capsules containing sealant mounted in said container, said cylinders being located initially adjacent the closure opposite to that through which the cannula may penetrate, the space within the tube and between the penetrable closure and the cylinders forming a first chamber for receiving blood from the cannula;

d. the upper cylinder having a specific gravity greater than the light phase of blood, the lower cylinder having a specific gravity less than the heavy phase of blood, and the combined cylinders plus capsules having an average specific gravity between the two phases of blood; and

e. the rupturable capsules containing sealant having a specific gravity between the two phases of blood and being interposed between the two cylinders;

f. said slidable cylinders being adapted to move automatically to float on the top of the interface between the phases while moderate centrifugal force is used to precipitate the cellular portion of the blood with the displaced plasma flowing into the upper portion of the first chamber;

g. the cylinders being further adapted to automatically stop in the plasma slightly above the heavy phase and rupture the capsules and release the sealant to form a seal slightly above the interface between the phases.

Claims (5)

1. A SEPARATOR ASSEMBLY CAPABLE OF SEPARATING BLOOD INTO ITS COMPONENT LIGHT PHASE OR PLASMA AND HEAVY PHASE OR CELLULAR PORTION COMPRISING: A. A CONTAINER HAVING AT LEAST ONE OPEN END WHICH IS ADAPTED TO RECEIVE BLOOD FOR SUBSEQUENT SEPARATION INTO A LIGHT PHASE AND A HEAVY PHASE, B. A CLOSURE SEALING THE OPEN END OF THE CONTAINER THE CLOSURE BEING FORMED OF A SELF-SEALING ELASTOMERIC MATERIAL WHICH IS PENETRABLE BY A CANNULA THROUGH WHICH BLOOD TO BE SEPARATED IS CONDUCTED IN THE CONTAINER, C. A PAIR OF CYLINDERS AND RUPTURABLE CAPSULES OF SEALANT SLIDABLY MOUNTED IN THE CONTAINER THE UPPER CYLINDER HAVING A SPECIFIC GRAVITY GREATER THAN THE LIGHT PHASE THE LOWER CYLINDER HAVING A SPECIFIC GRAVITY LESS THAN THE HEAVY PHASE AND THE COMBIED CYLINDERS PLUS CAPSULES HAVING A SPECIFIC GRAVITY BETWEEN THE TWO PHASES OF BLOOD, AND D. SAID RUPTURABLE CAPSULES OF SEALANT HAVING A SPECIFIC GRAVITY BETWEEN THE TWO PHASES OF BLOOD AND BEING INTERPOSED BETWEEN THE TWO CYLINDERS WHEREBY WHEN CENTRIFUGAL FORCE IS USED THE BLOOD SEPARATES INTO ITS LIGHT AND HEAVY PHASES THE CYLINDERS MOVE THROUGH THE LIGHT PHASE AND COME TO A STOP AT THE TOP OF THE HEAVY PHASE AND THE PRESSURE BETWEEN THE CYLINDERS RUPTURES THE CAPSULES AND RELEASES THE SEALANT TO FORM A SEAL SLIGHTLY ABOVE THE INTERFACE BETWEEN THE PHASES.
2. The separator of claim 1 wherein the said container comprises a tubular body open at each end in which closures formed of elastomeric material are mounted in sealing engagement with the tubular body and the cylinders are disposed adjacent one of said closures.
3. The separator of claim 1 wherein the sealant is gelatin.
4. A separator assembly capable of separating blood into its component light phase, plasma, and heavy phase, cellular portion comprising: a. a container for receiving blood having closures at each end for sealing the container; b. a pair of cylinders plus rupturable capsules of sealant slidably mounted in the container adjacent the upper closure, the upper cylinder having a specific gravity greater than the light phase, the lower cylinder having a specific gravity less than the heavy phase of blood, and the combined cylinders plus capsules having a specific gravity between the two phases of blood; and c. said rupturable capsules of sealant having a specific gravity between the two phases of blood and being interposed between the two cylinders, whereby when centrifugal force is used the blood separates into its light and heavy phases, the cylinders move through the light phase and come to a stop at the top of the heavy phase, and the pressure between the cylinders ruptures the capsules and releases the sealant to form a seal slightly above the interface between the phases.
5. A separator assembly capable of separating blood into its component light phase of plasma and heavy phase of cellular portion comprising: a. a tubular container having two open ends; b. resilient closure means adapted to close said ends with at least one said closure means capable of being penetrated by a cannula for filling with blood; c. a slidable pair of cylinders plus rupturable capsules containing sealant mounted in said container, said cylinders being located initially adjacent the closure opposite to that through which the cannula may penetrate, the space within the tube and between the penetrable closure and the cylinders forming a first chamber for receiving blood from the cannula; d. the upper cylinder having a specific gravity greater than the light phase of blood, the lower cylinder having a specific gravity less than the heavy phase of blood, and the combined cylinders plus capsules having an average specific gravity between the two phases of blood; and e. the rupturable capsules containing sealant having a specific gravity between the two phases of blood and being interposed between the two cylinders; f. said slidable cylinders being adapted to move automatically to float on the top of the interface between the phases while moderate centrifugal force is used to precipitate the cellular portion of the blood with the displaced plasma flowing into the upper portion of the first chamber; g. the cylinders being further adapted to automatically stop in the plasma slightly above the heavy phase and rupture the capsules and release the sealant to form a seal slightly above the interface between the phases.
US3909419A 1974-02-27 1974-02-27 Plasma separator with squeezed sealant Expired - Lifetime US3909419A (en)

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Cited By (57)

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US4012325A (en) * 1975-01-08 1977-03-15 Eastman Kodak Company Biological fluid dispenser and separator
US4055501A (en) * 1976-01-16 1977-10-25 Sherwood Medical Industries Inc. Fluid collection device with phase partitioning means
US4088582A (en) * 1976-01-16 1978-05-09 Sherwood Medical Industries Inc. Blood phase separation means
US4169060A (en) * 1977-10-25 1979-09-25 Eastman Kodak Company Blood-collecting and serum-dispensing device
US4369117A (en) * 1980-05-12 1983-01-18 American Hospital Supply Corporation Serum separating method and apparatus
GB2151161A (en) * 1983-12-05 1985-07-17 Sarstedt Kunststoff Centifuge container for plasma separation
US4818386A (en) * 1987-10-08 1989-04-04 Becton, Dickinson And Company Device for separating the components of a liquid sample having higher and lower specific gravities
US4877520A (en) * 1987-10-08 1989-10-31 Becton, Dickinson And Company Device for separating the components of a liquid sample having higher and lower specific gravities
US4946601A (en) * 1988-08-22 1990-08-07 Sherwood Medical Company Blood serum separator tube
US5236604A (en) * 1991-05-29 1993-08-17 Sherwood Medical Company Serum separation blood collection tube and the method of using thereof
US5269927A (en) * 1991-05-29 1993-12-14 Sherwood Medical Company Separation device for use in blood collection tubes
WO1996018897A1 (en) * 1994-12-13 1996-06-20 Coleman Charles M Separator float for blood collection tubes
US5736033A (en) * 1995-12-13 1998-04-07 Coleman; Charles M. Separator float for blood collection tubes with water swellable material
EP1006360A2 (en) * 1998-12-05 2000-06-07 Becton, Dickinson and Company Device and method for separating components of a fluid sample
US6390966B2 (en) * 2000-04-18 2002-05-21 Large Scale Proteomics Corporation Method for making density gradients
US20040166029A1 (en) * 1999-12-03 2004-08-26 Losada Robert J. Device for separating components of a fluid sample
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US20040182795A1 (en) * 2003-03-21 2004-09-23 Randel Dorian Apparatus and method for concentration of plasma from whole blood
US20050186120A1 (en) * 2002-05-03 2005-08-25 Randel Dorian Methods and apparatus for isolating platelets from blood
US20060032825A1 (en) * 2000-04-28 2006-02-16 Harvest Technologies Corporation Blood components separator disk
US20060151384A1 (en) * 2003-05-19 2006-07-13 Ellsworth James R Method and apparatus for separating fluid components
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US4012325A (en) * 1975-01-08 1977-03-15 Eastman Kodak Company Biological fluid dispenser and separator
US4055501A (en) * 1976-01-16 1977-10-25 Sherwood Medical Industries Inc. Fluid collection device with phase partitioning means
US4088582A (en) * 1976-01-16 1978-05-09 Sherwood Medical Industries Inc. Blood phase separation means
US4169060A (en) * 1977-10-25 1979-09-25 Eastman Kodak Company Blood-collecting and serum-dispensing device
US4369117A (en) * 1980-05-12 1983-01-18 American Hospital Supply Corporation Serum separating method and apparatus
GB2151161A (en) * 1983-12-05 1985-07-17 Sarstedt Kunststoff Centifuge container for plasma separation
US4818386A (en) * 1987-10-08 1989-04-04 Becton, Dickinson And Company Device for separating the components of a liquid sample having higher and lower specific gravities
US4877520A (en) * 1987-10-08 1989-10-31 Becton, Dickinson And Company Device for separating the components of a liquid sample having higher and lower specific gravities
US4946601A (en) * 1988-08-22 1990-08-07 Sherwood Medical Company Blood serum separator tube
US5236604A (en) * 1991-05-29 1993-08-17 Sherwood Medical Company Serum separation blood collection tube and the method of using thereof
US5269927A (en) * 1991-05-29 1993-12-14 Sherwood Medical Company Separation device for use in blood collection tubes
US5454958A (en) * 1991-05-29 1995-10-03 Sherwood Medical Company Method for sampling in a container having a material therein which separates from a barrier material
WO1996018897A1 (en) * 1994-12-13 1996-06-20 Coleman Charles M Separator float for blood collection tubes
US5736033A (en) * 1995-12-13 1998-04-07 Coleman; Charles M. Separator float for blood collection tubes with water swellable material
EP1006360A2 (en) * 1998-12-05 2000-06-07 Becton, Dickinson and Company Device and method for separating components of a fluid sample
EP1006360A3 (en) * 1998-12-05 2001-08-08 Becton, Dickinson and Company Device and method for separating components of a fluid sample
US6479298B1 (en) 1998-12-05 2002-11-12 Becton, Dickinson And Company Device and method for separating components of a fluid sample
US8524171B2 (en) 1999-12-03 2013-09-03 Becton, Dickinson And Company Device for separating components of a fluid sample
US20040166029A1 (en) * 1999-12-03 2004-08-26 Losada Robert J. Device for separating components of a fluid sample
US7947236B2 (en) 1999-12-03 2011-05-24 Becton, Dickinson And Company Device for separating components of a fluid sample
US9682373B2 (en) 1999-12-03 2017-06-20 Becton, Dickinson And Company Device for separating components of a fluid sample
US20110187021A1 (en) * 1999-12-03 2011-08-04 Becton, Dickinson And Company Device For Separating Components of a Fluid Sample
US9095849B2 (en) 1999-12-03 2015-08-04 Becton, Dickinson And Company Device for separating components of a fluid sample
US6390966B2 (en) * 2000-04-18 2002-05-21 Large Scale Proteomics Corporation Method for making density gradients
US20060032825A1 (en) * 2000-04-28 2006-02-16 Harvest Technologies Corporation Blood components separator disk
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