US3817838A - Method of detecting and measuring lipase activity - Google Patents

Method of detecting and measuring lipase activity Download PDF

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US3817838A
US3817838A US00230942A US23094272A US3817838A US 3817838 A US3817838 A US 3817838A US 00230942 A US00230942 A US 00230942A US 23094272 A US23094272 A US 23094272A US 3817838 A US3817838 A US 3817838A
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lipase
solution
fat
lipase activity
enzymes
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N Harris
E Reese
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United States Department of the Army
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase

Definitions

  • Lipase activity is detected and measured by incubating an aqueous solution extracted from a suspected fat-containing material with a solution of polyoxyethylene (20) sorbitan monolaurate and assaying the incubated solution to measure the reduction in ester groups of the polyoxyethylene (20) sorbitan monolaurate resulting from lipase induced deesterification.
  • This invention relates to a method for the detection and measurement of lipase activity which utilizes a novel test substrate.
  • hydrolytic decomposition or lipolysis is an undesirable phenomenon producing unwanted soapy or sour flavors in the product. This bydrolytic decomposition does not occur, however, unless some type of catalyst is present such as lipase enzymes or molds (fungi) which normally produce and release lipase enzymes during growth.
  • the fat or oil containing foodstuff is produced under such conditions as to be free of viable lipase-producing fungal microorganisms or active lipase materials, then hydrolytic decomposition of the fats or oils will not normally occur on storage. While treatment with moist heat will inactivate enzymes or microbes capable of producing such enzymes, a simple procedure is necessary to determine if lipases are present and if lipase-producers are totally inactivated during processing. A procedure, described by Arnulf Purr in an article entitled Test Paper for the Detection of Esterases in Animal and Plant Tissues and in Microorganisms appearing in Rev. Inter.
  • This invention is concerned with a novel procedure to test for the presence of lipase activity in fat-containing materials.
  • This procedure makes use of a novel test substrate, polyoxyethylene (20) sorbitan monolaurate, which is de-esterified by lipase enzymes capable of de-esterifying a medium-chain fatty acid ester.
  • a solution of the test substrate is combined with an aqueous extract of the material being analyzed and then incubated. Any lipase enzyme present will, under the incubation conditions, deesterify the test substrate. Reduction in ester content of the incubated mixture is determined colorimetrically by hydroxylamine assay.
  • Lipase activity is the ability of certain enzymes (lipases) to hydrolyze or de-esterify esters of medium-chain fatty acids. Lipases may be found naturally in animal or vegetable cells and are produced during the growth of certain fungal microorganisms. For hydrolytic decomposition of fat to occur, there must be present, in addition to the lipase enzymes, water in excess of 2% b weight. The fatty acids liberated as a result of the lipase induced de-esterification combine with available sodium and potassium ions to form soap-like compounds having noticeable soapy or sour taste properties.
  • the novel substrate used herein is polyoxyethylene (20) sorbitan monolaurate (POESM hereinafter) which is a mixture of laurate partial esters of sorbitol and anhydrides condensed with approximately 20 moles of ethyleneoxide for each mole or sorbitol and its monoand dianhydrides.
  • This material is soluble in water, ethyl alcohol, ethyl acetate, methanol and dioxane, but insoluble in mineral oil and in mineral spirits.
  • the POESM is further described and characterized at pages 525 and 526 of Food Chemical Codex, first edition, published by the National Academy of Sciences, National Research Council, 1966, and is commercially available as Tween 20, a product of Atlas Chemical Industries, Inc., Wilmington, Del.
  • the procedure of this invention which tests for the presence of lipase activity in a fat-containing material requires that the fat-containing material be mixed with and suspended in water so as to extract any Water-soluble lipase enzymes that may be present therein.
  • the fatcontaining suspension is then filtered and the aqueous filtrate is reserved.
  • a standard solution of the test substrate, POESM is prepared by adding 0.5 ml. of the substrate to water bufl'ered to a pH of 7.0 or 4.0 at 2.5% volume/volume.
  • the aqueous filtrate is added to the substrate solution and incubated under conditions which will allow de-esterification to occur. It is preferred that the incubation occur at temperatures of from 35 to 55 C. and for time periods ranging from 1 to 4 hours.
  • the incubated solution is then assayed to determine loss of ester in the test substrate.
  • the assayed procedure is described in detail by S. Hestrin, in the J. Biol. Chem., 180, 249, 261 (1949) in an article entitled Determination of Acetyl Content. This procedure is based upon the ability of esters to react with hydroxylamine quantitatively in an aqueous alkaline solution to form a purplebrown color, the intensity of which decreases with increasing substrate de-esterification.
  • the test can be done on either a qualitative or quantitative basis.
  • Pat is defined herein as a medium-chain fatty acid glyceride ester.
  • Medium-chain fatty acid is a saturated or unsaturated aliphatic monocarboxylic acid having a chain length of from 12 to 24 carbon atoms.
  • the enzymatic de-esterification of fat is especially significant in or unpalatable. This test procedure, therefore, will be especially desirable in assuring the necessary control of quality of fat-containing foodstuffs.
  • Cocconut and other high lauric oils or fats (triglycerides) such .aspalm kernel, babasu, tucum or mixtures thereof are highly sue, ceptible to lipase-hydrolysis.
  • coconut is frequently a component of candy and is likely to be stored for considerable lengths of time at ambient conditions; In order to successfully survive even short term storageundersuch conditions, it is essential that the coconut be free of lipase activity to prevent h'ydrolytic decompositionor foodstuffs, since I candies and can also be used in connection with other the off-flavors produced-render the product unacceptablede-esterification, resulting in a rancid or soap flavored product. 1 v I The following example illustrates in detail the proce dure of this invention.
  • EXAMPLE I 5 grams of tfinely ground coconut meat is combined with ml. of an aqueous solution of 0.15 M potassium chloride (pH 6.0). This suspension is agitated for 12 hours by continuous shaking at room temperature.. The suspension is filtered and the aqueous filtrate is reserved.
  • the test substrate (POESM) is added to water buffered to a pH of 7.0 at 2.5% volume/volume.
  • 0.5 ml. ofthe filtrate is added to 0.5 ml. of the substrate solutionand theresult ng mixture incubated in a waterbath at 35 C. for 3 hours.
  • the incubated solution is then assayed for ester content using the hydroxylamine assay procedure of S. Hestrin, to which reference has already been made.
  • equal volumes of 0.2 M hydroxylamine are mixed with 3.5 N sodium hydroxide. 1 ml.
  • fat-containing foods which could contain or be contaminated with lipase enzymes, such as intermediate moisture (7 to 12%) foods, dry fat containing food mixed with dry ground spices and certain cereal products.
  • a method for detecting lipase activity in a fatcontaining food stuff material which consists of the fol lowing steps in sequence:
  • This invention provides a solution to the lipase-detection problem in coconut containing foodstuffs such as White et al.:
  • said fat material is coconut oil.

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  • Chemical & Material Sciences (AREA)
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Abstract

LIPASE ACTIVITIY IS DETECTED AND MEASURED BY INCUBATING AN AQUEOUS SOLUTION EXTRACTED FROM A SUSPECTED FAT-CONTAINING MATERIAL WITH A SOLUTION OF POLYOXYETHYLENE (20) SORBITAN MONOLAURATE AND ASSAYING THE INCUBATED SOLUTION TO MEASURE THE REDUCTION IN ESTER GROUPS OF THE POLYOXYETHYLENE (20) SORBITAN MONOLAURATE RESULTING FROM LIPASE INDUCED DEESTERIFICATION.

Description

United States Patent 3,817,838 METHOD OF DETECTING AND MEASURING LIPASE ACTIVITY Norman E. Harris, Waltham, Mass., and Elwyn T. Reese, Caracas, Venezuela, assignors to the United States of America as represented by the Secretary of the Army No Drawing. Filed Mar. 1, 1972, Ser. No. 230,942 Int. Cl. G01n 31/14 US. Cl. 195-103.5 R 5 Claims ABSTRACT OF THE DISCLOSURE Lipase activity is detected and measured by incubating an aqueous solution extracted from a suspected fat-containing material with a solution of polyoxyethylene (20) sorbitan monolaurate and assaying the incubated solution to measure the reduction in ester groups of the polyoxyethylene (20) sorbitan monolaurate resulting from lipase induced deesterification.
The invention described herein may be manufactured, used and licensed by or for the Government for governmental purposes without the payment to us of any royalty thereon.
BACKGROUND OF THE INVENTION This invention relates to a method for the detection and measurement of lipase activity which utilizes a novel test substrate.
Fats and oils, upon storage, and in the presence of certain enzymes and moisture, undergo hydrolytic decomposition to form free fatty acids. Such free fatty acids combine with available sodium or potassium ions to form fatty acid salts, which are characterized as soaps. In foodstuffs containing fats and oils, hydrolytic decomposition or lipolysis is an undesirable phenomenon producing unwanted soapy or sour flavors in the product. This bydrolytic decomposition does not occur, however, unless some type of catalyst is present such as lipase enzymes or molds (fungi) which normally produce and release lipase enzymes during growth. If the fat or oil containing foodstuff is produced under such conditions as to be free of viable lipase-producing fungal microorganisms or active lipase materials, then hydrolytic decomposition of the fats or oils will not normally occur on storage. While treatment with moist heat will inactivate enzymes or microbes capable of producing such enzymes, a simple procedure is necessary to determine if lipases are present and if lipase-producers are totally inactivated during processing. A procedure, described by Arnulf Purr in an article entitled Test Paper for the Detection of Esterases in Animal and Plant Tissues and in Microorganisms appearing in Rev. Inter. Choc., 17, 567-571, December 1962, is based upon a test for the presence of non-specific esterases, i.e., enzymes capable of splitting esters of base fatt acids, e.g., acetates, and butyrates. Such enzymes are said to be commonly encountered along with lipase enzymes in animal and plant tissues and therefore a test for the former is said to indicate the presence of the latter. In this test procedure, free indoxyl is split from indoxyl acetate by esterases in the presence of moisture (pH 7.2- 7.5) with the free indoxyl being oxydized by atmospheric oxygen to form an insoluble blue pigment. This test, however, has been found to be too specific in that enzymes that attack and hydrolyze the acid chain would not necessarily hydrolyze the longer acid chains of 12 or more carbon atoms, such as found in lauric oils or fats. correspondingly, enzymes that would hydrolyze the 12 or more carbon chain fatty acid esters would not necessarily hydrolyze the shorter acetate chain. Therefore, this test procedure has not proved to be a reliable indication of the presence or absence of lipase activity.
SUMMARY This invention is concerned with a novel procedure to test for the presence of lipase activity in fat-containing materials. This procedure makes use of a novel test substrate, polyoxyethylene (20) sorbitan monolaurate, which is de-esterified by lipase enzymes capable of de-esterifying a medium-chain fatty acid ester. A solution of the test substrate is combined with an aqueous extract of the material being analyzed and then incubated. Any lipase enzyme present will, under the incubation conditions, deesterify the test substrate. Reduction in ester content of the incubated mixture is determined colorimetrically by hydroxylamine assay.
DESCRIPTION OF THE PREFERRED EMBODIMENTS Lipase activity is the ability of certain enzymes (lipases) to hydrolyze or de-esterify esters of medium-chain fatty acids. Lipases may be found naturally in animal or vegetable cells and are produced during the growth of certain fungal microorganisms. For hydrolytic decomposition of fat to occur, there must be present, in addition to the lipase enzymes, water in excess of 2% b weight. The fatty acids liberated as a result of the lipase induced de-esterification combine with available sodium and potassium ions to form soap-like compounds having noticeable soapy or sour taste properties.
The novel substrate used herein is polyoxyethylene (20) sorbitan monolaurate (POESM hereinafter) which is a mixture of laurate partial esters of sorbitol and anhydrides condensed with approximately 20 moles of ethyleneoxide for each mole or sorbitol and its monoand dianhydrides. This material is soluble in water, ethyl alcohol, ethyl acetate, methanol and dioxane, but insoluble in mineral oil and in mineral spirits. The POESM is further described and characterized at pages 525 and 526 of Food Chemical Codex, first edition, published by the National Academy of Sciences, National Research Council, 1966, and is commercially available as Tween 20, a product of Atlas Chemical Industries, Inc., Wilmington, Del.
The procedure of this invention which tests for the presence of lipase activity in a fat-containing material requires that the fat-containing material be mixed with and suspended in water so as to extract any Water-soluble lipase enzymes that may be present therein. The fatcontaining suspension is then filtered and the aqueous filtrate is reserved. A standard solution of the test substrate, POESM, is prepared by adding 0.5 ml. of the substrate to water bufl'ered to a pH of 7.0 or 4.0 at 2.5% volume/volume. The aqueous filtrate is added to the substrate solution and incubated under conditions which will allow de-esterification to occur. It is preferred that the incubation occur at temperatures of from 35 to 55 C. and for time periods ranging from 1 to 4 hours. The incubated solution is then assayed to determine loss of ester in the test substrate. The assayed procedure is described in detail by S. Hestrin, in the J. Biol. Chem., 180, 249, 261 (1949) in an article entitled Determination of Acetyl Content. This procedure is based upon the ability of esters to react with hydroxylamine quantitatively in an aqueous alkaline solution to form a purplebrown color, the intensity of which decreases with increasing substrate de-esterification. The test can be done on either a qualitative or quantitative basis.
Any fat-containing material may be tested for the presence of lipases according to this invention. Pat is defined herein as a medium-chain fatty acid glyceride ester. Medium-chain fatty acid is a saturated or unsaturated aliphatic monocarboxylic acid having a chain length of from 12 to 24 carbon atoms. The enzymatic de-esterification of fat is especially significant in or unpalatable. This test procedure, therefore, will be especially desirable in assuring the necessary control of quality of fat-containing foodstuffs. Cocconut and other high lauric oils or fats (triglycerides) such .aspalm kernel, babasu, tucum or mixtures thereof are highly sue, ceptible to lipase-hydrolysis. Coconut is frequently a component of candy and is likely to be stored for considerable lengths of time at ambient conditions; In order to successfully survive even short term storageundersuch conditions, it is essential that the coconut be free of lipase activity to prevent h'ydrolytic decompositionor foodstuffs, since I candies and can also be used in connection with other the off-flavors produced-render the product unacceptablede-esterification, resulting in a rancid or soap flavored product. 1 v I The following example illustrates in detail the proce dure of this invention.
EXAMPLE I 5 grams of tfinely ground coconut meat is combined with ml. of an aqueous solution of 0.15 M potassium chloride (pH 6.0). This suspension is agitated for 12 hours by continuous shaking at room temperature..The suspension is filtered and the aqueous filtrate is reserved. The test substrate (POESM) is added to water buffered to a pH of 7.0 at 2.5% volume/volume. 0.5 ml. ofthe filtrate is added to 0.5 ml. of the substrate solutionand theresult ng mixture incubated in a waterbath at 35 C. for 3 hours. The incubated solution is then assayed for ester content using the hydroxylamine assay procedure of S. Hestrin, to which reference has already been made. In the assay, equal volumes of 0.2 M hydroxylamine are mixed with 3.5 N sodium hydroxide. 1 ml.
types of fat-containing foods which could contain or be contaminated with lipase enzymes, such as intermediate moisture (7 to 12%) foods, dry fat containing food mixed with dry ground spices and certain cereal products.
We claim: f '1. A method for detecting lipase activity in a fatcontaining food stuff material which consists of the fol lowing steps in sequence:
(a) dispersing and agitating a quantity of said fatcontaining material in water to facilitate extraction of water-soluble components, (b) filtering the dispersion and reserving the aqueous filtrate, I, (c) combining a solution of polyoxyethylene (20) sorbitan monolaurate with the filtrate,
(d) holding the mixture'of step (0) under incubation conditions for sufiicient time to allow de-esterifica- I tion to occur, and
(e) assaying the mixture for loss, of ester material by ;.means of LhydrOXylamine assay wherein an aqueous alkaline solution of hydroxylamine is first added to said mixture and then an acidic solution of ferric chloride is added thereto to form a colored solution in the presence of ester material, the density 'of the .f"color being related to the amount of ester material in the solution.
2. A method according to claim 1 wherein said 'mixture is'incubated 'at a temperature ranging from 35 to of the incubated solution and 1 ml. of alkaline hydroxyla amine solution are combined. After one minute, 1 ml. of concentrated hydrochloric acid solution (1 volume hydrochloric acid, 2 volumes water) is added, followed by 1 ml. of a ferric chloride solution (0.37-M ferric chloride in 0.1 N hydrochloric acid). The density of activity was detected only in the coconut. The percent transmission for the coconut was 31%, for milk powder 8%, for chocolate liquor 8% and for cocoa 5%. n This invention provides a solution to the lipase-detection problem in coconut containing foodstuffs such as White et al.:
559C. and for a time ranging from 1 to 4 hours.
3. A' method according to claim 2 wherein said suspension filtrate and said test substrate are buffered to a pH of 7.0 or 4.0. 4. A method according to claim 3 wherein said fat is a glyceride ester of one or more medium-chain fatty acids.
5. A method according to claim 4 wherein said fat material is coconut oil.-
v References Cited Analytical Abstracts, Vol.1, 2781 (1954). Hestrin: J. Biol. Chem, 180, 249-261 (1949).
Prin. of Biochem 3rd ed. (1964), pp.
ALVIN E. 'rAriENHoL'rz, Primary Examiner
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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4311788A (en) * 1978-07-05 1982-01-19 Heuck Claus Christian Process for the quantitative determination of a serum protein in turbid serum and plasma samples
US4321364A (en) * 1980-04-17 1982-03-23 Minister For Public Works For The State Of New South Wales Preparation of soluble chromogenic substrates
US6312901B2 (en) 1996-07-08 2001-11-06 Burstein Technologies, Inc. Spatially addressable, cleavable reflective signal elements, assay device and method
US6331275B1 (en) 1996-07-08 2001-12-18 Burstein Technologies, Inc. Spatially addressable, cleavable reflective signal elements, assay device and method
US6342349B1 (en) 1996-07-08 2002-01-29 Burstein Technologies, Inc. Optical disk-based assay devices and methods
US20020106661A1 (en) * 1996-07-08 2002-08-08 Burstein Laboratories, Inc. Optical disk-based assay devices and methods
US20050214827A1 (en) * 1996-07-08 2005-09-29 Burstein Technologies, Inc. Assay device and method
US7347972B1 (en) 1998-07-22 2008-03-25 Jin Po Lee Multiple analyte assay device
EP3244189A1 (en) 2008-12-30 2017-11-15 Jin Po Lee Quantitative analyte assay device and method

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4311788A (en) * 1978-07-05 1982-01-19 Heuck Claus Christian Process for the quantitative determination of a serum protein in turbid serum and plasma samples
US4321364A (en) * 1980-04-17 1982-03-23 Minister For Public Works For The State Of New South Wales Preparation of soluble chromogenic substrates
US6312901B2 (en) 1996-07-08 2001-11-06 Burstein Technologies, Inc. Spatially addressable, cleavable reflective signal elements, assay device and method
US6331275B1 (en) 1996-07-08 2001-12-18 Burstein Technologies, Inc. Spatially addressable, cleavable reflective signal elements, assay device and method
US6342349B1 (en) 1996-07-08 2002-01-29 Burstein Technologies, Inc. Optical disk-based assay devices and methods
US20020106661A1 (en) * 1996-07-08 2002-08-08 Burstein Laboratories, Inc. Optical disk-based assay devices and methods
US20050214827A1 (en) * 1996-07-08 2005-09-29 Burstein Technologies, Inc. Assay device and method
US7347972B1 (en) 1998-07-22 2008-03-25 Jin Po Lee Multiple analyte assay device
EP3244189A1 (en) 2008-12-30 2017-11-15 Jin Po Lee Quantitative analyte assay device and method

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