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Plasma separator system

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US3654925A
US3654925A US3654925DA US3654925A US 3654925 A US3654925 A US 3654925A US 3654925D A US3654925D A US 3654925DA US 3654925 A US3654925 A US 3654925A
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tube
blood
collecting
plasma
end
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William J Holderith
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Becton Dickinson and Co
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Becton Dickinson and Co
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61BDIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00Detecting, measuring or recording for diagnostic purposes; Identification of persons
    • A61B5/15Devices for taking samples of blood
    • A61B5/150007Details
    • A61B5/150755Blood sample preparation for further analysis, e.g. by separating blood components or by mixing

Abstract

A blood-collecting tube of the evacuated type containing an anticoagulant receives through normal techniques whole blood to be tested. Thereafter, a cell-collecting tube is interconnected with the blood-collecting tube through the intermediary of a double-ended needle assembly including an external discoidal hub intermediate the ends thereof, such that the interior of both tubes communicate with one another. The tubes with the interconnecting needle assembly are then subjected to centrifugation with the cell-collecting tube being spaced radially outwardly relative to the blood-collecting tube and the axis of rotation. Upon generation of sufficient centrifugal forces, the plasma separates from the heavier constituents with the latter and particularly the blood cells being forced out into the cell-collecting tube and packed therein. The relative capacities of the tubes are such that in the blood-collecting tube only plasma will remain following completion of centrifugation. The needle assembly is then removed from the blood-collecting tube to effectively separate and remove plasma from the remaining constituents of the blood.

Description

United States Patent [151 3,654,925 51 Apr. 11,1972

Holderith [54] PLASMA SEPARATOR SYSTEM [72] Inventor: William J. Holderith, Wyckoff, NJ.

[73] Assignee: Becton, Dickinson and Company, East Rutherford, NJ.

[22] Filed: Sept. 23, 1969 [21] Appl. No; 860,191

[52] US. Cl ..128/272, 128/318 M [51] Int. Cl. ..A61j 01/00 [58] Field ofSearch ..l28/2,2l8 M,218D, 272

[56] References Cited UNITED STATES PATENTS 1,614,067 1/1927 Heublein ..128/272 1,928,998 10/1933 Kovacs ..128/272 2,342,214 2/1944 Perelson... ..128/272 UX 2,655,919 10/1953 Goodstein et a1. ..128/218 M 2,734,649 2/1956 Callahan et al. ....128/272 X 2,908,274 10/1959 Bujan ....128/272 3,200,813 8/1965 Christakis. ..128/2 3,221,741 12/1965 LeVeen ....128/272 3,490,437 l/1970 Bakondy et a1. ..128/272 X Primary Examiner-Joseph S. Reich Attorney-Kane, Dalsimer, Kane, Sullivan and Smith [5 7] ABSTRACT A blood-collecting tube of the evacuated type containing an anticoagulant receives through normal techniques whole blood to be tested. Thereafter, a cell-collecting tube is interconnected with the blood-collecting tube through the intermediary of a double-ended needle assembly including an external discoidal hub intermediate the ends thereof, such that the interior of both tubes communicate with one another. The tubes with the interconnecting needle assembly are then subjected to centrifugation with the cell-collecting tube being spaced radially outwardly relative to the blood-collecting tube and the axis of rotation. Upon generation of sufficient centrifugal forces, the plasma separates from the heavier constituents with the latter and particularly the blood cells being forced out into the cell-collecting tube and packed therein. The relative capacities of the tubes are such that in the bloodcollecting tube only plasma will remain following completion of centrifugation. The needle assembly is then removed from the blood-collecting tube to effectively separate and remove plasma from the remaining constituents of the blood.

1 Claims, 3 Drawing Figures PLASMA SEPARATOR SYSTEM BACKGROUND OF THE INVENTION In the testing of blood, it is often desired to obtain a sample of blood plasma, the clear, straw-colored liquid left when all formed elements in uncoagulated blood have been removed. To obtain plasma, it is common practice to draw blood into a tube containing an anticoagulant, centrifuge this sample, and remove the supernatant plasma either by decanting (pouring it off) or removing it with an aspirating device such as a pipette or syringe. These removal methods are not entirely satisfactory in that both require the tube to be opened and exposed to the air, thereby risking contamination. The decanting method also risks mixing of the formed elements with the plasma, rendering the plasma unusable. The transfer method is also unsatisfactory in that the sample may be contaminated by the transfer device, and the cost of the transfer device adds extra expense to the determination and analysis.

SUMMARY OF THE INVENTION It is, therefore, an object of this invention to provide a more effective system and procedure for removing plasma from whole blood after the sample has been centrifuged.

Another object is to provide a device and system of this type which permits the removal of the plasma portion without disturbing the packed red cells thus allowing the use of pure plasma in many tests wherein the presence of formed elements might render the test invalid.

A further object is to provide a system of this type which is essentially closed" in that neither portion of the original sample is exposed to the atmosphere.

BRIEF DESCRIPTION OF THE DRAWING FIG. 3 is a similar longitudinal sectional view showing theblood-collecting tube associated with the cell-collecting tube through the intermediary of the double-ended needle.

DESCRIPTION OF THE PREFERRED EMBODIMENTS The illustrated blood plasma collecting system includes a blood-collecting tube such as is described in US. Pat. No. 2,460,641 titled Blood Collecting Apparatus, granted Feb. 21, 1949 a cell-collecting tube 12, and an intermediate needle assembly 14 which may be either affixed to the stopper of the cell-collecting tube 12 or be constructed as a separate unattached assembly. Referring initially to the tube 10, it will be noted that a tubular body 16 of essentially cylindrical configuration includes a closed end 18 and an open end 20. Sealing the open end 20 is a penetrable self-sealable stopper 22. In accordance with conventional practice, the stopper 22 cooperates in maintaining a vacuum within the tube 10 which also conveniently receives an anticoagulant for whole blood. The stopper 22 includes an interiorly disposed plug portion 24 and an exterior radially extending flange portion 26 disposed against the open end 20. An exterior conical recess 28 facilitates penetration of the stopper 22 by a pointed needle. The interior of the plug portion 24 includes an inner end 30 forming the base of a truncated conical recess, the conical walls of which serve a funneling function in facilitating the complete removal of the solid constituents of whole blood during centrifugation in a manner to be described in detail below.

The cell-collecting tube 12, on the other hand, includes a tubular body portion 32 having a closed end 34 and an open end 36 across which a stopper 38 extends in sealing relationship. In this connection, the capacity of tube 10 relative to tube 12 should be such that all of the heavier constituents of the separated blood are accommodated and only a minimum amount of plasma will be present. The stopper 38 includes the usual plug portion 40 and flange portion 42. This stopper is also adapted to be penetrated and self-scalable. For purposes of this invention, the tube 12 need not be evacuated and may possess essentially the same capacity as tube 10, but is preferably smaller in volume.

The needle assembly 14 is provided with a double-ended cannula 44 having bevel pointed ends 46 and 48. A hub 50 is affixed to a cannula 44 intermediate its ends. The needle assembly 14 may conveniently form a part of the tube 12 as shown in FIG. 2 or be an independent assembly but necessary component or part of the plasma-collecting system of this invention.

In use, the blood-collecting tube 10 is employed together with a double-ended hypodermic needle (not shown) to collect a sample of venous or arterial blood in a manner similar to that disclosed in US. Pat. No. 2,460,641. As soon as convenient thereafter, the cell-collecting tube 12 is attached to tube 10 by inserting the bevel end 46 of cannula 44 through stopper 22, such that the parts assume the position illustrated in FIG. 3. The assembly is then placed in a centrifuge (not shown) and held therein in such a manner that centrifugal forces will act along the common axis of the assembly from the blood-collecting tube 10 towards the cell-collecting tube 12. The centrifuge is run for a sufficient period of time to enable the generated centrifugal forces to force the liquid in the tube 10 to displace the air in tube 12 and eventually urge the solid constituents of the blood and particularly the cells thereof to be packed within the tubular body 32 of cell-collecting tube 12 as shown in FIG. 3. The plasma passes through the cannula 44 into the tube 10 with a minor portion of the plasma remaining in tube 12. When centrifugation is complete the assembly is removed and the tube 10 separated from the needle assembly l4, and consequently the tube 12. Remixing of plasma and the fomied elements or solid constituents of the blood is prevented by removal of the connecting cannula, while the assembly is held vertically and contamination is prevented by maintaining a closed system which can be made entirely sterile if desired.

As stated in the foregoing, the conical recess 30 of stopper 22 avoids entrapment of red cells or other solid constituents in the upper tube 10 during centrifugation. It is also preferred that the bevel end 46 of cannula 44 not protrude into the conical recess 30 for this same reason but be disposed in the cylindrical extension 32 of this recess. By the same token, the bevel end 48 of cannula 44 should be disposed within the tube 12 and related to its internal capacity such that this bevel end be disposed in the plasma portion and not protrude into the packed cell portion following centrifugation.

In an alternate embodiment of the plasma separator system, tubes 10 and 12 may be of substantially equal size and capacity. With this in mind both tubes may be utilized to collect blood. However, the needle assembly 14 does not form part of tube 12 and is a separate unit which is thereafter employed to join the tubes together. This assembly is then centrifuged and the blood separated. The plasma and cells are then isolated one from the other and collected for the intended purposes.

Thus, the aforenoted objects and advantages are most effectively obtained. Although several preferred embodiments of the invention have been disclosed and described in detail herein, it should be understood that this invention is in no sense limited thereby and its scope is to be determined by that of his appended claims.

Iclaim:

l. A plasma separating system comprising in combination a blood-collecting tube comprising a tubular body having a closed end and an open end fitted with a needle penetrable stopper; said stopper extending across and covering the open end thereof; said blood-collecting tube having whole blood disposed therein; a cell-collecting tube comprising a tubular body having a closed end and an open end fitted with a stopper; said stopper extending across and closing the open end thereof; said cell-collecting tube having a volume such that the cells obtained from separating whole blood disposed in the blood-collecting tube would fill a major portion of the cell-collecting tube; a double-ended needle assembly mounted on the blood-collecting and cell-collecting tubes; said doubleended needle assembly having cannula and a hub disposed between the ends thereof, each end of the cannula having a beveled pointed end so as to penetrate through the stopper of the blood-collecting tube and the stopper of the cell-collecting tube so that the interior of each of said tubes is in fluid communication with each other whereby the cells contained in the whole blood are adapted to be separated by external forces on said system and when separated will substantially till a major portion of the cell-collecting tube by passing through the cannula of the double needle assembly and, at the same time, the liquid plasma portion of the whole blood will fill at least a portion of the blood-collecting tube, and said hub being adapted to engage with an exterior surface of said stopper on said cellcollecting tube such that the pointed end of the needle associated with the cell-collecting tube is disposed interiorly thereof and short of the cells of the blood collected therein following said separation.

Claims (1)

1. A plasma separating system comprising in combination a bloodcollecting tube comprising a tubular body having a closed end and an open end fitted with a needle penetrable stopper; said stopper extending across and covering the open end thereof; said bloodcollecting tube having whole blood disposed therein; a cellcollecting tube comprising a tubular body having a closed end and an open end fitted with a stopper; said stopper extending across and closing the open end thereof; said cell-collecting tube having a volume such that the cells obtained from separating whole blood disposed in the blood-collecting tube would fill a major portion of the cell-collecting tube; a double-ended needle assembly mounted on the blood-collecting and cell-collecting tubes; said double-ended needle assembly having cannula and a hub disposed between the ends thereof, each end of the cannula having a beveled pointed end so as to penetrate through the stopper of the blood-collecting tube and the stopper of the cell-collecting tube so that the interior of each of said tubes is in fluid communication with each other whereby the cells contained in the whole blood are adapted to be separated by external forces on said system and when separated will substantially fill a major portion of the cell-collecting tube by passing through the cannula of the double needle assembly and, at the same time, the liquid plasma portion of the whole blood will fill at least a portion of the blood-collecting tube, and said hub being adapted to engage with an exterior surface of said stopper on said cellcollecting tube such that the pointed end of the needle associated with the cell-collecting tube is disposed interiorly thereof and short of the cells of the blood collected therein following said separation.
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Cited By (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3866608A (en) * 1973-10-23 1975-02-18 Sorenson Research Co Aseptic suction collection system and method
US3939822A (en) * 1974-08-14 1976-02-24 Jack Markowitz Disposable blood collection and filtering device
US4230584A (en) * 1976-10-21 1980-10-28 Terumo Corporation Liquid separating composition and apparatus for applying said composition
US4811866A (en) * 1987-01-02 1989-03-14 Helena Laboratories Corporation Method and apparatus for dispensing liquids
US4818386A (en) * 1987-10-08 1989-04-04 Becton, Dickinson And Company Device for separating the components of a liquid sample having higher and lower specific gravities
US4824560A (en) * 1985-04-18 1989-04-25 Assaf Pharmaceutical Industries Ltd. Separation of materials from a liquid dispersion by sedimentation
US5555920A (en) * 1991-04-30 1996-09-17 Automed Corporation Method and apparatus for aliquotting blood serum or blood plasma
US5968018A (en) * 1996-10-30 1999-10-19 Cohesion Corporation Cell separation device and in-line orifice mixer system
US5997811A (en) * 1997-07-02 1999-12-07 Cohesion Technologies, Inc. Method for sterile syringe packaging and handling
US6132353A (en) * 1996-10-21 2000-10-17 Winkelman; James W. Apparatus and method for separating plasma or serum from the red cells of a blood sample
US20020169408A1 (en) * 1997-06-24 2002-11-14 Roberto Beretta Systems and methods for preparing autologous fibrin glue
US20040071786A1 (en) * 1997-06-24 2004-04-15 Grippi Nicholas A. Methods and devices for separating liquid components
US20060196885A1 (en) * 2005-02-21 2006-09-07 Biomet Manufacturing Corp. Method and apparatus for application of a fluid
WO2007021344A1 (en) * 2005-08-17 2007-02-22 Cascade Medical Enterprises, Llc Systems and methods for preparing autologous fibrin glue
US20080190857A1 (en) * 2005-03-22 2008-08-14 Cascade Medical Entrprises, Llc System and Methods of Producing Membranes
US20090250413A1 (en) * 2008-04-04 2009-10-08 Biomet Biologics, Llc Sterile Blood Separating System
US20090258056A1 (en) * 1997-06-24 2009-10-15 Cascade Medical Enterprises, Llc Systems and methods for preparing autologous fibrin glue
US20100288694A1 (en) * 2009-05-15 2010-11-18 Becton, Dickinson And Company Density Phase Separation Device
US8182769B2 (en) 2008-04-04 2012-05-22 Biomet Biologics, Llc Clean transportation system
US8313954B2 (en) 2009-04-03 2012-11-20 Biomet Biologics, Llc All-in-one means of separating blood components
US8328024B2 (en) 2007-04-12 2012-12-11 Hanuman, Llc Buoy suspension fractionation system
US8337711B2 (en) 2008-02-29 2012-12-25 Biomet Biologics, Llc System and process for separating a material
US8567609B2 (en) 2006-05-25 2013-10-29 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US8591391B2 (en) 2010-04-12 2013-11-26 Biomet Biologics, Llc Method and apparatus for separating a material
US8596470B2 (en) 2007-04-12 2013-12-03 Hanuman, Llc Buoy fractionation system
US8603346B2 (en) 2002-05-24 2013-12-10 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
WO2014019255A1 (en) * 2012-07-31 2014-02-06 上海科华检验医学产品有限公司 Vacuum blood collection pipe capable of directly separating serum and method thereof
US8783470B2 (en) 2009-03-06 2014-07-22 Biomet Biologics, Llc Method and apparatus for producing autologous thrombin
US8808551B2 (en) 2002-05-24 2014-08-19 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US8950586B2 (en) 2002-05-03 2015-02-10 Hanuman Llc Methods and apparatus for isolating platelets from blood
US9011800B2 (en) 2009-07-16 2015-04-21 Biomet Biologics, Llc Method and apparatus for separating biological materials
US9339741B2 (en) 2008-07-21 2016-05-17 Becton, Dickinson And Company Density phase separation device
US9556243B2 (en) 2013-03-15 2017-01-31 Biomet Biologies, LLC Methods for making cytokine compositions from tissues using non-centrifugal methods
US9642956B2 (en) 2012-08-27 2017-05-09 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US9682373B2 (en) 1999-12-03 2017-06-20 Becton, Dickinson And Company Device for separating components of a fluid sample
US9694359B2 (en) 2014-11-13 2017-07-04 Becton, Dickinson And Company Mechanical separator for a biological fluid
US9701728B2 (en) 2008-02-27 2017-07-11 Biomet Biologics, Llc Methods and compositions for delivering interleukin-1 receptor antagonist

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Cited By (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3866608A (en) * 1973-10-23 1975-02-18 Sorenson Research Co Aseptic suction collection system and method
US3939822A (en) * 1974-08-14 1976-02-24 Jack Markowitz Disposable blood collection and filtering device
US4230584A (en) * 1976-10-21 1980-10-28 Terumo Corporation Liquid separating composition and apparatus for applying said composition
US4824560A (en) * 1985-04-18 1989-04-25 Assaf Pharmaceutical Industries Ltd. Separation of materials from a liquid dispersion by sedimentation
US4811866A (en) * 1987-01-02 1989-03-14 Helena Laboratories Corporation Method and apparatus for dispensing liquids
US4818386A (en) * 1987-10-08 1989-04-04 Becton, Dickinson And Company Device for separating the components of a liquid sample having higher and lower specific gravities
US5555920A (en) * 1991-04-30 1996-09-17 Automed Corporation Method and apparatus for aliquotting blood serum or blood plasma
US6398705B1 (en) * 1996-10-21 2002-06-04 Manfred Grumberg Apparatus for separating plasma or serum from the red cells with a blood sample
US6132353A (en) * 1996-10-21 2000-10-17 Winkelman; James W. Apparatus and method for separating plasma or serum from the red cells of a blood sample
US5968018A (en) * 1996-10-30 1999-10-19 Cohesion Corporation Cell separation device and in-line orifice mixer system
US7745106B2 (en) 1997-06-24 2010-06-29 Cascade Medical Enterprises, Llc Methods and devices for separating liquid components
US20020169408A1 (en) * 1997-06-24 2002-11-14 Roberto Beretta Systems and methods for preparing autologous fibrin glue
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US20090203613A1 (en) * 1997-06-24 2009-08-13 Cascade Medical Enterprises, Llc Systems and methods for preparing autologous fibrin glue
US8491564B2 (en) * 1997-06-24 2013-07-23 Cascade Medical Enterprises, Llc Systems and methods for preparing autologous fibrin glue
US20090258056A1 (en) * 1997-06-24 2009-10-15 Cascade Medical Enterprises, Llc Systems and methods for preparing autologous fibrin glue
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US9682373B2 (en) 1999-12-03 2017-06-20 Becton, Dickinson And Company Device for separating components of a fluid sample
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US20060196885A1 (en) * 2005-02-21 2006-09-07 Biomet Manufacturing Corp. Method and apparatus for application of a fluid
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WO2014019255A1 (en) * 2012-07-31 2014-02-06 上海科华检验医学产品有限公司 Vacuum blood collection pipe capable of directly separating serum and method thereof
US9642956B2 (en) 2012-08-27 2017-05-09 Biomet Biologics, Llc Apparatus and method for separating and concentrating fluids containing multiple components
US9556243B2 (en) 2013-03-15 2017-01-31 Biomet Biologies, LLC Methods for making cytokine compositions from tissues using non-centrifugal methods
US9694359B2 (en) 2014-11-13 2017-07-04 Becton, Dickinson And Company Mechanical separator for a biological fluid

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