US3413097A - Automatic pipette-system arrangement, the action of which is controlled, for transferring liquid from one test to another - Google Patents

Automatic pipette-system arrangement, the action of which is controlled, for transferring liquid from one test to another Download PDF

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Publication number
US3413097A
US3413097A US456791A US45679165A US3413097A US 3413097 A US3413097 A US 3413097A US 456791 A US456791 A US 456791A US 45679165 A US45679165 A US 45679165A US 3413097 A US3413097 A US 3413097A
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United States
Prior art keywords
arrangement
test
pipette
holder
another
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Expired - Lifetime
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US456791A
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English (en)
Inventor
Jungner Olof Gunnar Hugo
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Centrala Automationslaboratoriet AB CALAB
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Centrala Automationslaboratoriet AB CALAB
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0213—Accessories for glass pipettes; Gun-type pipettes, e.g. safety devices, pumps
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids

Definitions

  • An automatic pipetting arrangement for transferring fluid from one test tube to another provides movement of a vertical pipette holder in successive axial, then lateral, and then axial steps, the axial movements taking place in opposite directions.
  • the arrangement includes a pair of rotatably driven lever arms each having a longitudinal slot therein.
  • a pair of pins disposed for movement within the slots and secured to the holder ride in an inverted U- shaped track and trace out the prescribed movement of the holder upon rotation of the lever arms.
  • the present invention relates to an automatic pipettesystem arrangement, the action of which is so controlled that the transfer of liquid from one test tube to another can be eifected automatically by means of said arrangement.
  • liquid is transferred from one test tube to another by means of a pipette.
  • a tube is used which takes the form of an upwardly and downwardly bent U, the one branch of which is used as a pipette whereas the other is immersed into a vessel containing fluid, which vessel for the purpose of siphoning a definite volume of fluid into the pipe can be lowered a certain distance corresponding to the amount of fluid to be drawn into said pipe thereby causing a vacuum in the pipe.
  • the present invention relates to an automatic siphoning arrangement using a pipette system in which both test tubes containing the various reagent liquids or substances can be added to the apparatus at the same time in pairs or in two parallel series, and comprises mainly in that the pipette, which is connected to a suction pipe, is arranged to be retained by a substantially vertically arranged holder, whichwith the assistance of at least one lever rotatable about a shaft at a right angle to the plane of the holder--can be brought first a lineal movement vertically upwards, thereafter a substantially lineal lateral movement at right angles, and finally once again a movement in a vertical direction but this time downwards, or vice versa.
  • the lever controlling the movement of the holder is thereby securely mounted at one end to the rotating shaft and at 3,413,097 Patented Nov. 26, 1968 its other end provided with a pin, by which the holder is controlled, which pin is arranged to be moved in a groove in the form of an upturned and downturned U in a plate suitably made of plastic-which plate at the same time supports the rotatable shaft-and partly displaceably arranged in a longitudinal track in the lever rotatably about the rotating shaft.
  • the holder is also thereby at its other end guided by a pin capable of being guided in another U-shaped groove, which pin in the same way as the above mentioned pin is displaceably mounted in a rotable lever which rotates together with a second shaft at right angles to the plane of the holder.
  • Both of the rotatable levers in order to attain the aforementioned movement of the pipette holder, are thereby connected so that they move synchronously.
  • a suitable embodiment of the invention consists in that a reversible motor is arranged on a plate arranged upon the same, which motor drives a gear which in turn drives two more gears of which one drives the one rotatable shaft and the other the other rotatable shaft for both the aforementioned rotatable levers.
  • End position contacts are arranged so as to obtain the oscillating reciprocating movements of the motor, which contacts are capable of being mounted on the aforementioned plate and which are arranged to be actuated by cam members on any of the aforementioned gears.
  • the time taken for the horizontal movement of the holder arrangement can be made longer or shorter so that needed time remains at the disposal for releasing the suction pipe of the pipette suction arrangement and for a possible connection of compressed air for the purpose of flushing out the fluid in the other pipette suction pipe.
  • FIG. 1 shows the arrangement in perspective seen obliquely from the front
  • FIG. 2 shows the interior of the arrangement, i.e. the driving arrangement for moving the tube up and down.
  • the suction pump has not been reproduced in any of the figures for the purpose of illustration.
  • Two similarly U-shaped tracks 1 and 2 are arranged on the front of the arrangement on its upper and lower section, on which track the guide pins (not shown) of the suction pipe holder 3, slide.
  • the guide pins are further guided by means of slots 4 and 5 disposed in the rotating levers 6 and 7.
  • the levers 6 and 7 are secured on the shafts 8 and 9 respectively (FIG. 2).
  • each of said shafts 8 and 9 are fitted with gear wheels 10 and 11 respectively.
  • Both the gear wheels are identical with respect to the number of teeth, teeth-size and diameter.
  • One of the gear wheels is provided with a cam mounted in a suitable position, which cam actuates end contacts 12 and 13 respectively in the driving circuit for a driving motor intended for the shafts 8 and 9 as well as for the suction pipe holder 3.
  • the contacts are thereby actuated when the suction pipe holder occupies its lowest right hand or left hand position respectively.
  • the end contacts 12 and 13 and possibly a timing device (not shown) influence the motor current in a desired manner.
  • the motor 14 has reversible rotation direction and drives, via a gear reduction system, the cog wheel 15 situated between the cog wheels 10 and 11, said cog wheel 15 driving said gear wheels 10 and 11.
  • the operation of the arrangement is as follows:
  • Placed at a suitable height under the left hand branch of the tracks 1 and 2 in FIG. 1 is a suitable shaped vessel containing the test fluid which is to be transferred to the test tube for example, which test tube is placed under the right hand branch in FIG. 1 of the tracks 1 and 2.
  • a test tube has been indicated by a broken line.
  • the suction pipe is lowered into the vessel containing the test fluid by rotating the levers 6 and 7, driven by means of the motor and gear wheels, counter-clockwise.
  • the motor 14 stops for a certain period of time, possibly an adjusted period, which is decisive for the siphoned volume of fluid, whereafter the motor in restarted in the reverse direction of rotation, the suction pipe is raised, moved to the right, lowered and remains, possibly only a certain time, directly above the test tube.
  • the suction arrangement which has been in operation the whole time, is disconnected, the test fluid runs out into the test tube, the motor 14 is restarted, once again in a reversed movement of direction, at the same time as the suction arrangement is reconnected and the suction tube once again moved down into the vessel containing the test fluid. In between times, the just filled test tube is replaced by a new test tube, possibly automatically, and the process is repeated.
  • An automatic pipetting arrangement for transferring fluid from one test tube to another, comprising pipetting means connected to a suction source, a substantially vertical holder for holding said pipetting means, at least two pins for mounting said holder, said pins forming right angles with said holder, at least two levers, each lever having a longitudinal slot therein for receiving one of said pins, said slots permitting movement of said pins along the lengths of said levers, shaft means for rotatably driving said levers, said shaft means forming a right angle with said holder, stationary track means comprising inverted U-shaped tracks for engaging with each of said pins, and means for driving said shaft means and said associated lever means in synchronism so as to impart three successive movements to said holder, said successive movements comprising an axial movement, a lateral movement and an axial movement.
  • An automatic pipetting arrangement as claimed in claim 1 further comprising compressed air means for blowing out the fluid in a test tube during the lateral movement of said holder, the length of the yoke portion of said inverted U-shaped tracks being sufficient to permit the blowing out operation.
US456791A 1964-05-21 1965-05-18 Automatic pipette-system arrangement, the action of which is controlled, for transferring liquid from one test to another Expired - Lifetime US3413097A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE6171/64A SE320208B (de) 1964-05-21 1964-05-21

Publications (1)

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US3413097A true US3413097A (en) 1968-11-26

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US456791A Expired - Lifetime US3413097A (en) 1964-05-21 1965-05-18 Automatic pipette-system arrangement, the action of which is controlled, for transferring liquid from one test to another

Country Status (5)

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US (1) US3413097A (de)
DE (1) DE1598220B2 (de)
FR (1) FR1436321A (de)
GB (1) GB1095272A (de)
SE (1) SE320208B (de)

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3536449A (en) * 1967-04-13 1970-10-27 Thomas W Astle Serial dilution machine
US3753657A (en) * 1971-06-11 1973-08-21 Micromedic Systems Inc Automatic test tube transporter and sample dispenser having solid state controls
US3807467A (en) * 1970-04-08 1974-04-30 Sherwood Medical Ind Inc Medicament filling unit
US4234023A (en) * 1977-03-02 1980-11-18 Olympus Optical Co., Ltd. Liquid feeder for automatic culture apparatus
US4405315A (en) * 1982-04-23 1983-09-20 Stephen R. Ash Spike exchanger for continuous ambulatory peritoneal dialysis
WO1984000895A1 (en) * 1982-09-10 1984-03-15 Baxter Travenol Lab Automatic connection and disconnection
WO1984002849A1 (en) * 1983-01-18 1984-08-02 Travenol Europ Res & Dev Automatic connection device
US4498358A (en) * 1981-09-22 1985-02-12 Raque Food Systems Inc. Oscillating head filler device
US4541829A (en) * 1982-09-10 1985-09-17 Baxter Travenol Laboratories, Inc. Automatic connection and disconnection
US20090012455A1 (en) * 2007-07-05 2009-01-08 Baxter International Inc. Dialysis system having supply container autoconnection
US20090012451A1 (en) * 2007-07-05 2009-01-08 Baxter International Inc. Peritoneal dialysis patient connection system
US20090012459A1 (en) * 2007-07-05 2009-01-08 Baxter International Inc. Peritoneal dialysis patient connection system using ultraviolet light emitting diodes
US20100130920A1 (en) * 2008-11-21 2010-05-27 Baxter International Inc. Dialysis machine having auto-connection system with roller occluder
US8764702B2 (en) 2007-07-05 2014-07-01 Baxter International Inc. Dialysis system having dual patient line connection and prime
US9586003B2 (en) 2007-07-05 2017-03-07 Baxter International Inc. Medical fluid machine with supply autoconnection
US10293091B2 (en) 2007-07-05 2019-05-21 Baxter International Inc. Dialysis system having an autoconnection mechanism

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2624656A (en) * 1946-03-28 1953-01-06 Harry C Andrews Remote control pipetting unit
US3143393A (en) * 1959-06-18 1964-08-04 Luc Donald De Seguin Des Hons Apparatus for automatically performing chemical operations and similar or related operations
US3178266A (en) * 1960-10-07 1965-04-13 Warner Lambert Pharmaceutical Materials handling apparatus
US3192968A (en) * 1962-07-02 1965-07-06 Warner Lambert Pharmaceutical Apparatus for performing analytical procedures
US3193359A (en) * 1962-07-02 1965-07-06 Warner Lambert Pharmaceutical Apparatus for conducting analytical procedural steps
US3193358A (en) * 1962-07-02 1965-07-06 Warner Lambert Pharmacentical Automated analytical apparatus

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2624656A (en) * 1946-03-28 1953-01-06 Harry C Andrews Remote control pipetting unit
US3143393A (en) * 1959-06-18 1964-08-04 Luc Donald De Seguin Des Hons Apparatus for automatically performing chemical operations and similar or related operations
US3178266A (en) * 1960-10-07 1965-04-13 Warner Lambert Pharmaceutical Materials handling apparatus
US3192968A (en) * 1962-07-02 1965-07-06 Warner Lambert Pharmaceutical Apparatus for performing analytical procedures
US3193359A (en) * 1962-07-02 1965-07-06 Warner Lambert Pharmaceutical Apparatus for conducting analytical procedural steps
US3193358A (en) * 1962-07-02 1965-07-06 Warner Lambert Pharmacentical Automated analytical apparatus

Cited By (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3536449A (en) * 1967-04-13 1970-10-27 Thomas W Astle Serial dilution machine
US3807467A (en) * 1970-04-08 1974-04-30 Sherwood Medical Ind Inc Medicament filling unit
US3753657A (en) * 1971-06-11 1973-08-21 Micromedic Systems Inc Automatic test tube transporter and sample dispenser having solid state controls
US4234023A (en) * 1977-03-02 1980-11-18 Olympus Optical Co., Ltd. Liquid feeder for automatic culture apparatus
US4498358A (en) * 1981-09-22 1985-02-12 Raque Food Systems Inc. Oscillating head filler device
US4405315A (en) * 1982-04-23 1983-09-20 Stephen R. Ash Spike exchanger for continuous ambulatory peritoneal dialysis
WO1983003764A1 (en) * 1982-04-23 1983-11-10 Handt Alan E Spike exchanger for continuous ambulatory peritoneal dialysis
WO1984000895A1 (en) * 1982-09-10 1984-03-15 Baxter Travenol Lab Automatic connection and disconnection
US4541829A (en) * 1982-09-10 1985-09-17 Baxter Travenol Laboratories, Inc. Automatic connection and disconnection
WO1984002849A1 (en) * 1983-01-18 1984-08-02 Travenol Europ Res & Dev Automatic connection device
US8083709B2 (en) 2007-07-05 2011-12-27 Baxter International Inc. Dialysis method having supply container autoconnection
US8597230B2 (en) 2007-07-05 2013-12-03 Baxter International Inc. Dialysis system having supply container autoconnection
US20090012459A1 (en) * 2007-07-05 2009-01-08 Baxter International Inc. Peritoneal dialysis patient connection system using ultraviolet light emitting diodes
US11931497B2 (en) 2007-07-05 2024-03-19 Baxter International Inc. System and method for preparing peritoneal dialysis fluid at the time of use
US7736328B2 (en) 2007-07-05 2010-06-15 Baxter International Inc. Dialysis system having supply container autoconnection
US20100229366A1 (en) * 2007-07-05 2010-09-16 Baxter International Inc. Dialysis method having supply container autoconnection
US20090012455A1 (en) * 2007-07-05 2009-01-08 Baxter International Inc. Dialysis system having supply container autoconnection
US8157761B2 (en) 2007-07-05 2012-04-17 Baxter International Inc. Peritoneal dialysis patient connection system
US8197087B2 (en) 2007-07-05 2012-06-12 Baxter International Inc. Peritoneal dialysis patient connection system using ultraviolet light emitting diodes
US8257299B2 (en) 2007-07-05 2012-09-04 Baxter International Dialysis methods and systems having autoconnection and autoidentification
US8469545B2 (en) 2007-07-05 2013-06-25 Baxter Healthcare Inc. Peritoneal dialysis connection system and method for using ultraviolet light emitting diodes
US20090012451A1 (en) * 2007-07-05 2009-01-08 Baxter International Inc. Peritoneal dialysis patient connection system
US8764702B2 (en) 2007-07-05 2014-07-01 Baxter International Inc. Dialysis system having dual patient line connection and prime
US8911109B2 (en) 2007-07-05 2014-12-16 Baxter Healthcare Inc. Peritoneal dialysis connection system and method for using ultraviolet light emitting diodes
US8986243B2 (en) 2007-07-05 2015-03-24 Baxter International Inc. Peritoneal dialysis patient connection system
US11730868B2 (en) 2007-07-05 2023-08-22 Baxter International Inc. Dialysis system having an autoconnection mechanism
US9586003B2 (en) 2007-07-05 2017-03-07 Baxter International Inc. Medical fluid machine with supply autoconnection
US11311657B2 (en) 2007-07-05 2022-04-26 Baxter International Inc. Dialysis system for mixing treatment fluid at time of use
US10293091B2 (en) 2007-07-05 2019-05-21 Baxter International Inc. Dialysis system having an autoconnection mechanism
US10335532B2 (en) 2007-07-05 2019-07-02 Baxter International Inc. Dialysis system having autoidentification mechanism
US9931454B2 (en) 2008-11-21 2018-04-03 Baxter International Inc. Dialysis machine having auto-connection system with roller occluder
US9044544B2 (en) 2008-11-21 2015-06-02 Baxter International Inc. Dialysis machine having auto-connection system with roller occluder
US20100130920A1 (en) * 2008-11-21 2010-05-27 Baxter International Inc. Dialysis machine having auto-connection system with roller occluder

Also Published As

Publication number Publication date
DE1598220A1 (de) 1969-07-31
SE320208B (de) 1970-02-02
DE1598220B2 (de) 1970-12-10
GB1095272A (en) 1967-12-13
FR1436321A (fr) 1966-04-22

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