US3259012A

US3259012A - Microscope having bridge type support arm for body tube and focusing mechanism

Info

Publication number: US3259012A
Application number: US55340A
Authority: US
Inventors: Locquin Marcel
Original assignee: Inventions Finance Corp
Current assignee: Inventions Finance Corp
Priority date: 1960-09-12
Filing date: 1960-09-12
Publication date: 1966-07-05
Anticipated expiration: 1983-07-05

Description

y 1966 M. LOCQUIN 3,259,012

MICROSCOPE HAVING BRIDGE TYPE SUPPORT ARM FOR BODY TUBE AND FOCUSING MECHANISM Filed Sept. 12, 1960 4 Sheets-Sheet 1 MARCEL LOCQUIN 72: BY Mw M ATTORNEY y 5, 1956 M. LOCQUIN 3,259,012

MICROSCOPE HAVING BRIDGE TYPE SUPPORT ARM FOR BODY TUBE AND FOCUSING MECHANISM Filed Sept.

4 Sheets-Sheet 2 I i m 4 M HHHHQWQQ E FIG. 8

F. |G 6 INVENTOR MARCEL LOCQUIN BY MWV'M ATTORNEY y 5, 1966 M. LOCQUIN 3,259,012

MICROSCOPE HAVING BRIDGE TYPE SUPPORT ARM FOR BODY TUBE AND FOCUSING MECHANISM Filed Sept. 12, 1960 4 Sheets-Sheet 5 X FIG. 9

FIG. l4d

INVENTOR MARCEL LOCQUIN FIG. \7 BY M ATTORNEY July 5, 1966 LOCQUIN 3,259,012

MICROSCOPE HAVING BRIDGE TYPE SUPPORT ARM FOR BODY TUBE AND FOCUSING MECHANISM Filed Sept. 12, 1960 4 Sheets-Sheet 4 FIG. 20 h FIG. 204' 156M155 AM 136 as;

z 142 I I M 2 V I33 FIG. l5

INVENTOR FIGZO MARCEL LOCQUIN BY qM ATTORNEY United States

Patent

3,25%,012 MICROSCOPE HAVING BRIDGE TYPE SUPPORT ARM FOR BODY TUBE AND FGCUSING MECH- ANISM Marcel Locquin, Montreuil, France, assignor, by mcsne assignments, to Inventions Finance Corporation, a corporation of Delaware Filed Sept. 12, 1960, Ser. No. 55,340 Claims. (CI. 8839) This invention relates to microscopes and more particularly to an improved microscope in which the body tube is carried by a bridgelike arm spanning the base, the body tube being provided with a coarse adjustment focusing mechanism and the arm with a fine adjustment focusing mechanism so arranged as to provide extreme precision and stability of focus. Further improvements and numerous other advantages will be pointed out hereinafter.

In conventional micro-scopes, the optical system is carried by a stand customarily consisting of an arm mounted on a supporting base through the medium of one or more adjusting devices such as a rack and pinion, or a lever, making it possible to adjust the position of the objective in relation to the object under examination, the latter being carried by a stage rigidly afiixed to the stand, under which there is located the source of illumination and the optical system which concentrates the light on the sub- 'ect. J The movements of the objective and its support arm must beable to be precisely controlled; consequently, the adjusting system, such as the rack and pinion or lever, has to be made with very great precision. This precision machining generally requires a truing operation which is very expensive, and with use the adjust mechanism can easily get out of adjustment due to wear.

In French Patent No. 1,162,627, the applicant has proposed replacing the above-mentioned device with a new system having no overhang, in which the optical system is coupled to an arm resting at both ends on the base of the stand, one of these being connected to an elastic hinge associated with the base of the stand, and the other being connected to a device making it possible to act upon this hinge, which is placed under spring bias so as to bring about the vertical movement of the objective or objectives in relation to the base supporting the object.

This arrangement affords numerous advantages over the rack and pinion system. However, when in use, the tension resulting from the presence of an elastic hinge, and which is necessary to the movements of the piece supporting the lens, causes deformations of the assembly containing this piece, which are detrimental to the stability of focus.

Accordingly, it is a primary object of the present invention to provide a microscope structure which will remedy the above-cited defect and obviate the disadvantages of microscopes of conventional construction.

Another object of the invention is to provide a microscope having a base and a bridgelike body tube carrying arm, the body tube being provided with a coarse focusing mechanism and the arm with a fine adjustment focusing mechanism, whereby to provide precise and stable focus adjustments and exceptional precision.

Yet another object of the invention is to provide a microscope having the constructional features outlined above in which automatic focusing to accommodate change of slides regardless of their thickness is also provided.

Still another object of the invention is to provide a microscope having the constructional features outlined above which is simple of construction, easy to operate and economical to produce.

Patented July 5, 1966 A further object of the invention is to provide a microscope of the character indicated in which is incorporated an improved illumination system combined with improved objectives comprising the optical system.

Yet a further object of the invention is to provide a microscope of the construction indicated above which is susceptible to use with a great variety of accessory devices to enable semimicroprojection, macrography, microdissection, photomicrography, cinemic-rography, tomophotometry and similar functions.

Still another object of the invention is to provide a microscope of the type outlined above whose center of gravity is always in the optical axis even when equipped wit-h heavy accessory devices whereby to permit a more precise and stable functioning in any of its universal applications.

The novel features that are considered characteristic of the invention are set forth with particularity in the appended claims. The invention itself, however, both as to its organization and its method of operation, together with additional objects and advantages thereof, will best be understood from the following description of specific embodiments when read in connection with the accompanying drawing, wherein like reference characters indicate like parts throughout the several figures and in which:

FIG. 1 is a perspective view of a microscope stand constructed in accordance with the invention fitted with optics and binocular observation tubes;

FIG. 2 is a plan view of the microscope with certain parts broken away to reveal sectional details;

FIG. 3a is a cross sectional view taken along line 3a3a, FIG. 2, and looking in the direction of the arrows;

FIG. 3b is a cross sectional view taken along line 3b3b, FIG. 2;

FIG. 30 is a cross-sectional view taken along line 3c 30 of FIG. 2;

FIG. 4 is an enlarged sectional view taken axially of the fine focusing mechanism for the microscope.

FIG. 4a is a plan view of a structural element taken from line 441-411 of FIG. 4;

FIG. 5 is a cross sectional view taken axially of the coarse focusing mechanism for the microscope;

FIG. 6 is a fragmentary diagrammatical cross section of the prefocusing mechanism taken along plane 6-6 of FIG. 5 and showing its upper level position in broken lines;

FIG. 7 is a sectional view taken along line 77 of FIG. 5;

FIG. 8 is a perspective view of the microscope condenser holder;

FIG. 9 is a cross sectional view of a hemispherical condenser lens;

FIG. 10 is a cross sectional view of a spherical condenser lens;

FIG. ll is a cross sectional view of a double lens condenser;

FIG. 12 is a cross sectional view of a dark ground catadioptric Cassegrain condenser;

FIG. 13 is a cross sectional view of a bifocal c'atadioptric condenser;

FIG. 14a is a cross sectional view of a phase contrast condenser;

FIG. 14bd are cross sectional diagrammatic views of a modified phase contra-st condenser showing the paths of rays when one of the lens segments is moved axially with respect to the other lens segment;

FIG. 15 is a cross sectional view of a self-illuminating condenser;

FIG. 16 is a perspective view of the mirror holder and mirror;

FIG. 17 is an axial cross sectional view of an external light source with cooling device;

'FIG. 18 is a diagrammatical view of the binocular tubes and support in section;

FIG. 19 is a perspective view of the microscope with television camera fitted on the body tube; and

FIGS. 2011-1 are cross sectional views of various catadioptric objectives.

The microscope stand The stand according to the present invention is of the type in which the body tube is carried by an arm overspanning its base, and it is characterized principally by the fact that the arm is in the form of a bridge resting on the base, or on an element mounted on the latter, according to the system which shall be called a plane-line-point support in this application.

The advantages of this plane-line-point system are its great simplicity of construction, requiring no precision or truing, and its insensitivity to shocks and to accidental or thermal deformations.

According to one particular embodiment, the planeline-point support is achieved by causing the narrow end, or nose, of the bridge to be supported by a movable adjusting member connected to the stage support or, more simply, to the base, whereas the broad end of the said bridge rests upon the base or upon an element mounted on the latter through the medium of two balls, one of which is lodged in a hollow conical recess and the other is engaged in a V-groove.

Referring now to the drawings and more particularly to FIGS. 1, 2, 3a-3c, a preferred embodiment of the stand according to the invention is constituted by a base 3 bearing an upright 11 constituting a holder for the stage support 12 which rests, at its other extremity, on the central fixed column of the fine focusing mechanism designated by the general reference 13. On the stage support 12, on the side opposite mechanism 13, there is mounted an insert 4 upon which rests the upper portion of the stand which has the general form of a bridge designated by reference number 2. In the upper part of this bridge is mounted the optical device designated by the reference number 1.

The nose portion 5 of bridge 2 overspanning the stage support assembly 12, rests upon that portion of the stage support which overlies the fine focusing mechanism 13. The nose portion 6 of the arm or bridge 2 rests upon the insert 4 by means of a point disposed substantially at the place near one end designated as 7 and, near the other end, by means of a line support located substantially at the position marked 8. The nose portion 5 reposes on the stage support 12 by means of a plane support located at the position indicated by the arrow P.

The insert 4 at position 7 is provided with an opening 19' having a shoulder 20 (FIG. 3b). A socket 26 provided with a corresponding shoulder is lodged in opening 19'. Socket 26 comprises a frusto-conical recess in which a ball 7 is seated. The ball is retained by a socket 2 1 lodged in corresponding part of the bridge portion 6. Socket 21 also comprises a frustoconical recess. The assembly of the two sockets with frustoconical recesses constitutes, with ball 9, the point support centered on the center of the ball which is provided between insert 4 and bridge 6.

At the are designated as 8 in FIG. 1, the line support is created between the inserted piece 4 and portion 6 of the bridge. The construction is illustrated in FIG. 30. This support also comprises a socket 22 of exterior form substantially similar to that of the socket 20, but in which the frustoconical recess for the ball 9 is replaced by a V- groove 19. Ball 9 is capped by another socket 21 lodged in the corresponding location of the bridge portion 6.

Between the point and line supports, the insert 4, which is affixcd in an appropriate manner, not shown, to the stage support, is fastened in an elastic fashion, to bridge 6, for example, by means of a bolt 32, FIG. 3a, screwed into a tapped hole 25 provided in the bridge. The bolt 32 passes through

holes

27 and 28 provided in the insert 4 and the stage support plate and bears a spring 29, one extremity of which rests against a step 30 in hole 27, and the other against a washer 3-1, placed under the head of bolt 32. This retaining assembly has the purpose simply of preventing bridge extremity 6 from being accidentally pulled away from the base, and it may be dispensed with without impeding the operation of the assembly.

Rough focusing mechanism In the upper horizontal portion of bridge 2, an opening 6 7 is provided, in which the rough focusing mechanism is engaged, FIG. 5. The driving member of this mechanism consists of a knurled nut 66 that can rotate about its axis but cannot shift axially by reason of engagement of shoulders carried in the nut and part 6 9. The internal thread as of nut 66 meshes with the external thread of a strip 68 that is aifixed by means of screws 70 to the body tube 71. The strip 68 slides in the vertical groove 68a formed in the sleeve 68b which is firmly fixed to the bridge arm 2.

The body tube is disposed perpendicularly to the stage support 12 and is equipped at 72 with classical means for the locking of the objective turret, which are not illustrated. The upper portion of tubular element 7 1 comprises a cylindrical flange 64 in which an elastic

click detent mechanism

63, 73 of known type is provided for the mounting of an inclined and interchangeable binocular tube 1, which is desired, can also be a monocular tube or a photographic tube, or a combination of a binocular tube with a tube for photography.

The rackless rough focusing movement 18 is based on the nut and screw system and has a travel of about 25 mm. It is irreversible even under the heaviest load. Therefore, the microscope will support the heaviest of equipment. An end-of-travel stop is located opposite the prefocusing stop 74 and is made adjustable in any suitable manner.

Neither grease nor oil nor graphite need be used in the movements. Lubrication is assured for the life of the instrument by an ant-ifriction substance impregnating the metal, which in this case is molbdenum bisulfide applied in aerosol form. This type of movement, furthermore, is self-adjusting for wear, like the fine focusing system to be described.

The threaded strip 68 is an element that is different from a rack. This sleeve permits vertical movement and guides it, while preventing any transverse play; it furthermore permits the achievement of a much less rapid movement than would be provided by a classical rack and pinion movement. During assembly, if the microscope is focused on an object, and the relative position of the

stops

74 and 75 is adjusted to the thickness of the slide, a useful prefocusing device is obtained. This prefocusing (FIG. 6) can be adjustably set according to the type of objective used; makes it possible for the operator, by placing the slide between the two nylon stops, to obtain automatic compensation for the thickness of slides. To do .this, the operator brings the stops into light contact with the slide by means of the rough focusing mechanism 18.

Fine focusing mechanism The fine focusing movement 13 acts on the nose of the bridge, and the bridge displacement resulting therefrom is on an are which always coincides with the tangent in the depth of field of the object under examination, no matter what the magnification might be. The theoretical lateral movement is thus always less than the power of resolution of the instrument, and consequently is imperceptible. The mechanism of the movement, is differential, and therefore practically impossible to wear out. Its speed is approximately microns per turn; its travel is 2.5 mm., and it is accurate to within 0.1 micron without backlash. Furthermore, take-up for any wear is automatic since the thrust is always against the same side of the threads.

In FIG. 4, the mechanism of the fine focusing movement is represented in axial section, and this figure illustrates the plane support (indicated by the arrow P in FIG. 1) between the base and the nose of the bridge. This plane support is provided more particularly between the nose 5 of the bridge and an abutment 43 which comes to bear on a flange 44 which will be described in connection with the mechanism for operating the fine focusing system. The latter comprises an elongated tubular core 48 screwed at 46 to the base 3 of the stand. The core 48 is equipped on the lower portion of its periphery with a thread 47. On the upper portion of the core periphery, which has a smaller diameter than the portion bearing thread 47, there is placed a sleeve 49, the upper end of which terminates in the flange 44 mentioned above. Sleeve 49 is prevented by a key 50 from turning in relation to the tubular core 48.

Around the assembly which has just been described, there is mounted a differential nut 51 comprising at its lower portion a thread 52 in engagement with thread 47, and at its upper part an internal thread 53 in engagement with the external thread 54 provided on the periphery of the sliding sleeve 49.

By means of a key 55, the differential nut 51 is made to rotate with a knurled cylinder 56 constituting the operating member of the device.

The pitch of thread 54 is smaller than the pitch of thread 47, so that a large rotation of the knurled cylinder 56 and consequently of the differential nut, corresponds to a small axial displacement of sleeve 49 and a small movement of abutment 43 and consequently of bridge nose 5.

The core 48 has a longitudinal bore 56 with a shoulder 57, and in this bore is lodged a rod 58, whose upper extremity 59 is yoked to a fork 60 borne by the screw 61 screwed into the nose 5 of the bridge. On its lower extremity, rod 58 bears a washer 62a which constitutes an abutment for a spring 63 mounted around rod 58. The other extremity of this spring bears on the step 57. The spring 63 may be dispensed with because it has no effect on the operation of the mechanism other than to provide a cushioning means.

To improve the stability of the bridge, it is expedient to separate the plane support of the plane-line point system from the fine focusing movement. To accomplish this, a washer half 33 hinged at 35 to the other half washer 36 is inserted between

abutments

43 and 34, the latter resting on the movable sleeve flange 44. Washer portion 36 is firmly aflixed to the stage support 12 by screw 37. The hinged washer assembly enables only part 33 to flex vertically. By its presence and its great horizontal rigidity, it prevents any reaction of the pos sible play in the movement on the nose of the bridge.

The stages The stage holder 12 can support in addition to the stage 14, FIG. 1, of the microscope, such accessories as electrode holders, microdissectors, microman-ipulators and thermometer probes. It is oval in its simplest form, and circular when it is necessary, to provide room for bulky accessories, such as micromanipnlators,

The stages mentioned above may be either fixed stages, stages that are movable by greasy friction, stages with built-in carriages, or independent-carriage stages, or last- 1y, movable stages driven automatically for the automatic exploration of the object, particularly for the purpose of counting.

The simplest fixed stage is square, rectangular or round.

The greasy friction stages 14 are constituted by two pieces with perfectly flat and lightly lubricated surfaces in contact. When the two pieces are placed on one another, atmospheric pressure opposes their free movement and retards the movements of the hand of the operator, thus demnltiplying his control action. The object in this case is advantageously held by magnetic clamps constituted by springs aflixe-d to a magnetized cube of metal, the assembly 'being affixed to the stage made of steel or soft iron.

Other stages, such as independent-carriage or built-in carriage stages of a classical type, may be used.

The optical system The optical system with which the stand is equipped is of a new type, utlizing the objectives illustrated in FIGS. 2061-201. The objectives of FIGS. 20d-20i operate when the object is in air or in an immersion liquid, whereaes the objectives in FIGS. 20b and c operate only when an air space is interposed between the object and the objective. The microscope thus equipped with mirror optics in this new form presents, in relation to other microscopes, the following advantages: operation possible in ultraviolet, infrared aberration, spherical aberration reduced to the minimum, and low cost.

The objective, which may be of the dry type or of the so-called immersion type, is characterized by the fact that it is constituted, mainly, of a lens whose convex face, situated on the object side, is covered with a coating of a completely reflective material comprising an uncoated area at its apex; its plane or spherical face situated on the image side comprises an opaque central area and is covered on at least a portion of its remaining area with a simple or complex semi-reflective coating. In addition to the advantage of perfect achromatism, this objective offers the advantage over the complex objectives mentioned above, and over the classical mirror objectives, that it simplifies the mechanical mounting and provides an extremely small central obturation which is all the smaller as the magnification is greater.

The objective illustrated in FIG. 20a is constituted by a piano-convex lens 131. The convex face situated on the side of the object 132 is covered with a fully reflective coating or mirror surface 133, with the exception of area 134 situated at the apex of the convex surface facing the object. The central portion of the plane face is covered with an opaque material 135 preventing the direct passage of rays through the lens in the axial zone, the remainder of the plane face being covered with a semi-reflective material 136'.

A ray 137 issuing from the object 132 undergoes an initial reflection at 138. The reflected ray in turn undergoes another reflection at 139 and then passes out of the objective at along the line 141 parallel to the axis of the objective, the image forming at infinity.

The perfect achromatism of such an objective is due to the fact that the light rays traverse all the air-to-glass surfaces at perpendicular incidence, so that there is no refraction, and the reflections on the mirrors do not introduce any chromatic aberration.

In the embodiment according to FIG. 20b, the objective is again constituted by a plane-convex lens 131a, the convex face of which, on the object side, is provided with a reflective surface 133 except for a portion 134a situated at its apex, which constitutes a spherical depression, the center of which coincides substantially with the object point 132.

With the exception of an uncoated annular peripheral area 142 and a central area bearing an opaque material 135, the plane face is covered with a semi-reflective coating 136. All the rays issuing from the object 132 undergo reflections on the

surfaces

133 and 136 and pass out perpendicularly to the plane face to form an image at infinity.

The embodiment of the objective represented in FIG. 20c differs from the one in FIG. 20b only in the fact that the lens 131b is a double convex lens. The radius of the convex face comprising the opaque spot surrounded by the semi-reflective coating 136 is centered on the image plane which, in this case, is situated at a finite distance.

The convex face situated on the side of the object 132 is again covered with a reflective material 133, with the exception of a central spherical zone 134b situated on the object side. In the case of an objective constituted by a biconvex lens, the magnification of the objective depends on the radius of curvature of the convex face provided with the reflective material.

The objective in FIG. 20d differs from that of FIG. 200 in that the spherical depression 134 situated on the object side is replaced by flat portion 134d situated at the apex of the convex face nearest the object, this flat portion being designed to be connected to the object by a drop of liquid of appropriate index equal to that of the glass forming the lens.

The objective of FIG. 20e is in conformity with any one of the types described above, as for example that of FIG. 20a, but associated with two lenses 143 and 144- designed to correct simple spherical aberration in case of large magnifications and large apertures.

The objective of FIG. 20 is in conformity with any one of the preceding types, but an annular phase plaque 145 of a known type is placed on the semi-reflective surface or is excavated therein.

The objective in FIG. 20g comprises a semi-reflective surface of an interferential nature 146 covering preferably the entire aperture, for the purpose of obtaining, in addition to reflection, a certain modulation in phase and wavelength of the light, to produce a certain color interest in the image.

The objective of FIG. 20h is similar to those described above except that a frustoconical glass front layer 152 has been added.

FIG. 201 illustrates a condenser 153 formed similar to the objective 131i and symmetrically disposed with respect to the object 132.

The described mirror lenses of FIG. 20 constitute perfectly achromatic objectives, can be manufactured with the classical materials, as for example glass, fused silica, fluorine, thallium iodobromide, etc., and can be used not only with rays of the visible spectrum, but also with rays outside of this spectrum. The semi-reflective coatings can be metallic, dielectric, or interferential.

In the case of phase contrast operation, it is advantageous to make the annular phase plaque in one of the following manners:

By removal of a circular portion of the semi-metallization at 142, FIG. 201';

By superimposed dephasing dielectric and absorptive metallic deposition;

By a succession of alternate dielectric and metallic depositions to produce the appropriate dephasing when the rays are reflected.

In all cases it is advantageous to replace the metallic coatings with high-index coatings such as titanium oxide or antimonium sulfide or a combination of them so as to improve the reflectivity.

Illumination When the object under examination is transparent, it can be illuminated in the conventional fashion by a condenser, reflecting mirror and external lamp, the lamp being able to be aflixed, in this case, on the circular emplacement 17 in FIG. 1. An external source of light may be of any type, but it is advantageous (FIG. 17) to make one of small size by using direct metallic contact to cool it. This contact is made either by means of stacked elastic fins 107 or by a mat of metal wool encased between two hemispheres, one of them being the reflector of the light. The assembly is connected to the base of the stand by a metal piece fastened to a lodgment provided for this purpose.

To avoid the classical defects of mirror holders, which are great diflioulty of adjustment and instability, the mirror holder in FIG. 16 has only one degree of liberty:

rotation, which prevents all instability without the need 8 for locking. The mirror 105 is surface-aluminized, which prevents any double image of the field diaphragm. It can be equipped with a cold mirror that reflects only visible light, this cold mirror being a multiple-layer interferential filter transparent to infrared and ultraviolet.

The condenser holder 16, FIGS. 1 and 9, is removable by unscrewing the 3 setscrews 10 and rotating it slightly to the left. The condenser comprises a cylinder 76 in which the lens is mounted and equipped for rapid rough adjustment by pushing .it upwardly or downwardly, and the fine adjustment by rotation. A ball is urged by a spring into a spiral groove; if the cylinder is pushed upward without rotating it, a rapid movement is obtained, and if it is rotated, a relatively slow movement is obtained, guided by the ball in the spiral groove. It can furthermore be centered by means of two orthogonal centering screws 78.

The actual optical systems which may be mounted on top of the condenser holder are the following:

A hemisphere 79, FIG. 9, a simple device but one that entails aberration;

Or a complete sphere 30, FIG. 10, also a simple device, but better than the hemisphere;

Or a classical system of lenses comprising at least two

lenses

81 and 82, associated together to reduce the ab berations (FIG. 11);

Or a dark field condenser, as for example a paraboloid or a Cassegrain device 83, FIG. 12;

Or a phase contrast condenser (FIGS. I la-14d) like the one to be described below;

Or a mixed catadioptric device such as a bifocal condenser 84, FIG. 13.

This is a mixed catadioptric assembly: a mirror and lens combined into a single element. It has no chromatic aberration at the mirror focus. The mirror focus can be used for powerful objectives. The lens focus can be used for low-power objectives. The two foci always coexist,

and this prevents the use of this device to form images.

By shifting the condenser from one focus 85 to the other 86, all the intermediate field and aperture adjustments are obtained, rendering unnecessary the usual iris diaphragm customarily used with condensers.

Furthermore, it Will be noted that, at each of the foci, the object is illuminated by the other focus along a thin axial beam, which actually amounts to the permanent superposition of a highly diaphragmed illumination, which gives the contrast, on a wide-open illumination which provides the separating power. The fringeless contrast obtained is therefore better than that obtained with an ordinary Abbe condenser and Koehlers principle.

The operation of condenser 84 is very simple. Since it is reduced to a single axial movement, it must be perfectly centered on the optical axis, and to this end it is fitted to the centering mount.

When it is desired to combine the source and the condenser in a single element, the so-called illuminating condenser can be constructed as shown in FIG. 15. The stage support designated by the general reference 99, comprising a central opening 103 into which there engages a cylindrical element 96, in the end of which is lodged a clear or groundglass sphere or ellipsoid 80. In the bottom of cylinder 96 is located a holding element 101 for socket 102 into which is screwed a bulb 161 having an integral convex lens. The socket-holding element 101 is capable of axial adjustment in the cylindrical element 96 by means of circular grooves 93 capable of coming into engagement with a toric element forming a spring. The variation of the relative position of the spherical or ellipsoidal lens and of the lens incorporated into the bulb provides, in simple fashion, an illuminating condenser of variable focus and aperture, which is far superior to the known spherical optical systerns.

Preferably, the groove 93 will be constructed in spiral form and will be engaged by a ball 100 in the tubular element 96; rotating the socket holding element will then cause it to be displaced axially. v

This assembly, which can be screwed into the greasyfriction round stage, comprises in a single unit the equivalent of a condenser and lamp with reflector. The bulb is powered at low voltage and can be fed by a flashlight battery or by a fixed power transformer that can supply several stands on the laboratory table, or by a transformer that can be controlled by a rheostat. Only bulbs with correctly centered filaments should be used.

To operate the microscope on phase contrast, a special condenser shown in FIG. 14a may be used. In this figure, the stage support'plate 12 may be seen, and the snap locking device 99 serving to hold in place a tubular element 92 substantially similar to the tubular element 96 of FIG. 15, but in the top of which a meniscus lens 91 is inserted. In this tubular element, there is a guided sleeve 94 terminating in a meniscus lens 90. The axial distance between the two meniscuses can be regulated by means of the spiral groove 93 provided in the periphery of the sleeve 94 and co-operating with a ball 100 carried by the tubular element. By making the sleeve 94 rotate about its axis, it is caused to shift axially, thus changing the distance between the two meniscuses, which are arranged so as to permit the light to pass in the form of an annular slot of variable thickness, so as enable the user to adjust the illumination precisely, according to the structure of the objects being examined.

An improvement of this phase contrast condenser is obtained by making two

mirrors

88, 89 with multiple concentric reflective areas (FIGS. 14b, 14c, 14d), the diameters of these areas being such that, by varying the distance between the two mirrors by a suitable amount, the operator can shift from the optical set-up 14b to c and then to d. This eliminates the need for cumbersome and costly so-called turret condensers.

Interferential filters may be placed between the light source and the condenser, particularly the rectangular polychroma-tic filters, which are interferential filters that operate by multiple band transmission. Being placed in a gimbal mount which engages into grooves provided for this purpose in the mirror holder, the filter is inclinable and retractable. By tilting, the pass bands of the long wavelengths are shifted towards the short wavelengths. It is thus always possible to make a pass band of the filter coincide with one of the \colors of the object and thus strengthen it in relation to all the others, without the object being illuminated with monochromatic light. Such filters are also recommended as generalpurpose contrast filters on naturally or artificially colored objects.

In addition to the filter, a circular lodgement on the mirror holder accepts fixed filters or a polarizing filter. The polarizing equipment of the stand is completed, in this latter case, by an analyzer and, if desired, with compensators placed between the body tube proper of the microscope and the observation tubes.

Light conductors, viz., bundles of fine glass fibers insulated optically from one another by a substance having a Weaker index of refraction than that of the glass of which they are made, can be used to conduct the light from the source to the object or from the source to the condenser. The use of such conductors is of greater interest in the case of opaque objects. The vertical illuminator of a classical type is connected to the light conductor, and the light is made to encircle the objective by means of a circular fanning of the glass fibers around it.

Temperature control When it is desired to observe objects at constant temperature, it is useful to enclose the free space between the bridge and the stage support and heat it at constant te perature by means of a heating resistance. This heating resistance is an actual stove incorporated into the microscope, not a simple heating stage which could not assure a precise, known ternperature at the level of the object.

Two transparent plastic hoods connect the bridge to the stage support, thus isolating the preparation and the objectives from the ambient air. The preparation and the objectives therefore are no longer a cold point close to the object. A fixed contact thermometer controls through a relay the heating resistance connected to the power line.

The nosepiece The nosepiece is a turret 97 placed on the body tube 71 to normally carry six objectives 95. The nose turret can be remotely controlled by a ratchet system, the control box, which is independent of the microscope, then comprising a presetting knob and a pushbutton for starting. After selecting the objective which one desires to use, one pushes the pushbutton and the turret revolves until the selected objective is facing the object.

The observation tubes The interchangeable observation tubes may be conventional, right monocular, inclined monocular, binocular and photographic binocular eye pieces. However, the present invention contemplates the use of a new and improved binocular mirror tube. The difference in quality which is observed, between the microscope image furnished by a classical upright tube and the one obtained with an inclined binocular tube, is due to the insertion into the optical trajectory of prisms for the division and deflection of the beam.

To completely eliminate these ditficulties, a binocular mirror tube 119 is used in which the beams do not pass through any mass of glass, excepting theone of minimum thickness that splits the beam in two, the tube being sealed by-flat glasses treated against reflections, see FIG. 18.

A longitudinally movable carriage 117 bears the two mirrors, one for the division 112, and one for the deflection 111 of the beam. To this carriage there is connected a taper 115 which simultaneously separates or brings together the eyepiece tubes 113 through the medium of piece 116. Since the two angle mirrors 109 and 110 are fixed, it is the position of the carriage 117 which determines both the separation of the two optical exit axes b and b and the synchronized mechanical separation of the eyepiece tubes.

Accessories Televisi0n.The connection of a television network to the microscope is desirable for teaching and research, particularly nuclear research, see FIG. 19. The camera is placed vertically on the stand by means of the intermediate piece without any ocular; the bridge form of the stand permits such mounting. The illumination of the microscope must be created by means of a lamp with an achromatic reflector and a bright bulb. The bifocal condenser is recommended, especially at low magnifications. The objectives must be of the largest possible aperture and must be devoid of spherical and chromatic aberration. It is advantageous to equip the microscope with the cold mirror already described. It is advantageous for the movements of focusing and of the changing of the objectives of the microscope to be remotely controlled. In addition to the remotely controlled stage and turret, the focusing movements can be remotely controlled by a mechanical device or by an electro-mechanical device, both of them being known and therefore not illustrated.

Measurements and counting To measure with precision the diameters of very small objects, and to count them, automatically or not, according to their diameter, a photometric discriminator is used. This is a compensator operating by polarized light, which permits the image to be divided in a continuously variable ratio. It serves also as a micrometer of great precision for the measurement of object diameters, and as a photometric discriminator for the counting of particles and for classifying them according to dimensions. This discriminator may be constituted of a classical Bravais compensator that is movable along the optical axis and connected to a scale for gauging its position.

It will be apparent from the above description that the improved microscope of the present invention may be utilized with many other accessory devices to accomplish such functions as microdissection, photomicrography with automatic control of light intensity, cinemicrography with automatic actuation of the movie camera, tomophotometry, and the like. In each instance, the microscope comprises a firm base with the center of gravity falling in the optical axis and the stability of the focus and ease of focusing contribute materially to successful accomplishment of the desired function.

Although certain specific embodiments of the invention have been shown and described, it is obvious that many modifications thereof are possible. The invention, therefore, is not to be restricted except insofar as is necessitated by the prior art and by the spirit of the appended claims.

What is claimed is:

1. A microscope comprising in combination a base, a body tube and a body tube carrying arm in the form of a bridge spanning the base, said arm being supported from both sides of said base in no more than three localized bearing areas, said bearing areas being localized into a point, a line, and a small area plane, said bridgelike arm resting at one end on a movable driving piece connected to the base by a fine adjustment focusing mechanism for elevating and lowering said end of the arm, and the other end of said arm resting upon two spaced ball bearings, one seated in the base in a conical opening and the other seated in a straight line channel having a V-shaped cross section, said fine adjustment tocusing mechanism comprising an elongated tubular core affixed at one end to said base, the exterior of one portion of said core having threads of a selected pitch, a sleeve slidable on another portion of the core and having external threads of a slightly different pitch, an elongated nut having separate internal sections with threads of different pitches meshing with the threads of said core and sleeve, and means moved by said sleeve in engagement with said driving piece to move the latter and said arm upon turning movement of said nut.

2. A microscope according to claim 1 wherein said conical opening and V-shaped cross section channel for seating the ball bearings are provided in a separate piece fastened to said base and securing a stage support plate thereto, and additional resilient means is provided to press said arm and said separate piece toward each other to hold the ball bearings seated.

3. A microscope according to claim 1 wherein each of said ball bearings is capped by a conical retainer secured to said arm, wherein said body tube includes eye piece means and carries an objective turret defining an optical axis, a spheriodal achromatic objective lens characterized by a convex face situated on the object side and which is coated with a reflective material except for a small area surrounding the optical axis, the other face of said lens having an opaque area in its center surrounded for at least a portion of its surface with a semireflective coating, and wherein said microscope further includes means for illuminating an object placed in said axis, said illuminating means comprising a tubular support coaxial with said optical axis, a light condenser in the form of a ball secured in one open end of said support, a lamp having an integral lens formed therein supported by a socket within the said support and means for axially adjusting the lamp and its socket within the said support.

4. A microscope according to claim 1 wherein a body tube receiving opening is provided in the central portion of said arm, said body tube being supported in said opening by a rough focusing mechanism and having means for detachably coupling a turret for objectives at its lower extremity and monocular or binocular observation tubes at its upper extremity.

5. A microscope according to claim 4 wherein said rough focusing mechanism comprises an internally threaded operating ring rotatably mounted on said arm and above said opening by means preventing axial movement thereof, a sleeve in said opening and fixed to said arm slideably receiving said body tube, and a longitudinal strip having external threads meshing with the internal threads of said operating ring inset in a longitudinal groove of said sleeve and aifixed to said body tube.

6. A microscope according to claim 5 wherein a pair of adjustable stops is additionally provided for mutual engagement to prefocus the microscope to compensate for differences in the thickness of the object to be viewed, said stops being respectively secured to fixed and moving parts of said rough focusing mechanism.

7. A microscope according to claim 1 wherein said fine adjustment focusing mechanism is further comprised of a fluted operating tube surrouding said nut and keyed thereto, said operating tube being supported between said base and a stage supported above the base in such manner as to permit rotation without axial movement of the operating tube.

8. A microscope according to claim 1 wherein is additionally provided resilient means urging said arm toward said base.

9. A microscope according to claim 8 wherein said resilient means comprises a shouldered opening positioned longitudinally within said core, a rod traversing said opening and secured in said core opening and surrounding said rod, said spring being comprised between the shoulder of the core opening and holding means affixed to the other end of said rod.

10. A microscope according to claim 1 wherein said bridgelike arm is centrally provided with an opening, a body tube adjustably supported in said opening to define a substanially vertical optical axis which coincides with the center of gravity of the microscope, and means for coupling accessory devices to said body tube in such manner as to coincide the centers of gravity of such devices with the optical axis of the microscope.

References Cited by the Examiner UNITED STATES PATENTS 262,634 8/1882 Yawman 88-39 1,110,266 9/1914 Kaufmann 88-39 1,461,367 7/1923 Ott et al. 88-40 1,934,874 11/1933 Neuwirth 88-39 2,166,034 7/1939 Allison 88-39 2,360,268 10/1944 Ott 88-39 2,503,389 4/1950 Host 88-40 2,687,670 8/1954 Locquin 88-39 2,691,918 10/1954 Robins et al 88-40 X 2,707,417 5/1955 Bouwers et al. 88-39 X 2,930,287 3/1960 Franks 88-39 FOREIGN PATENTS 1,222,290 1/1960 France.

972,971 12/ 1959 Germany.

473 1866 Great Britain. 817,452 7/ 1959 Great Britain.

OTHER REFERENCES Norris et al.: Reflecting Microscopes, article in Journal of the Optical Society of America, vol. 41, February 1951, pp. 118 and 119 cited.

Strong: Procedures in Experimental Physics, Textbook published 1946, pp. 585 and 586 cited.

DAVID H. RUBIN, Primary Examiner.

EMIL G. ANDERSON, Examiner.

Claims

1. A MIRCROSCOPE COMPRISING IN COMBINATION A BASE, A BODY TUBE AND A BODY TUBE CARRYING ARM IN THE FORM OF A BRIDGE SPANNING THE BASE, SAID ARM BEING SUPPORTED FROM BOTH SIDES OF SAID BASE IN NO MORE THAN THREE LOCALIZED BEARING AREAS, SAID BEARING AREAS BEING LOCALIZED INTO A POINT, A LINE, AND A SMALL AREA PLANE, SAID BRIDGELIKE ARM RESTING AT ONE END ON A MOVABLE DRIVING PIECE CONNECTED TO THE BASE BY A FINE ADJUSTMENT FOCUSING MECHANISM FOR ELEVATING AND LOWERING SAID END OF THE ARM, AND THE OTHER END OF SAID ARM RESTING UPON THE SPACED BALL BEARINGS, ONE SEATED IN THE BASE IN A CONICAL OPENING AND THE OTHER SEATED IN A STRAIGHT LINE CHANNEL HAVING A V-SHAPED CROSS SECTION, SAID FINE ADJUSTMENT FOCUSING MECHANISM COMPRISING AN ELONGATED TUBULAR CORE AFFIXED AT ONE END TO SAID BASE, THE EXTERIOR OF ONE PORTION OF SAID CORE HAVING THREADS OF A SELECTED PITCH, A SLEEVE SLIDABLE ON ANOTHER PORTION OF THE CORE AND HAVING EXTERNAL THREADS OF A SLIGHTLY DIFFERENT PITCH, AN ELONGATED NUT HAVING SEPARATE INTERNAL SECTIONS WITH THREADS OF DIFFERENT PITCHES MESHING WITH THE THREADS OF SAID CORE AND SLEEVE, AND MEANS MOVED BY SAID SLEEVE IN ENGAGEMENT WITH SAID DRIVING PIECE TO MOVE THE LATTER AND SAID ARM UPON TURNING MOVEMENT OF SAID NUT.