US3164279A - Test tube closure - Google Patents

Test tube closure Download PDF

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Publication number
US3164279A
US3164279A US3164279DA US3164279A US 3164279 A US3164279 A US 3164279A US 3164279D A US3164279D A US 3164279DA US 3164279 A US3164279 A US 3164279A
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Prior art keywords
tube
inner
closure
skirt
cap
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082Test tubes per se
    • B01L3/50825Closing or opening means, corks, bungs
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D51/00Closures not otherwise provided for
    • B65D51/16Closures not otherwise provided for with means for venting air or gas
    • B65D51/1605Closures not otherwise provided for with means for venting air or gas whereby the interior of the container is maintained in permanent gaseous communication with the exterior
    • B65D51/1622Closures not otherwise provided for with means for venting air or gas whereby the interior of the container is maintained in permanent gaseous communication with the exterior by means of a passage for the escape of gas between the closure and the lip of the container mouth
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/08Flask, bottle or test tube
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0684Venting, avoiding backpressure, avoid gas bubbles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/04Closures and closing means
    • B01L2300/046Function or devices integrated in the closure
    • B01L2300/048Function or devices integrated in the closure enabling gas exchange, e.g. vents

Description

Jan. 5, 1965 E. J. TOWNS TEST TUBE CLOSURE Filed Oct. 16, 1963 United States Patent Office 3,164,279 TEST TUBE CLGSURE Edward J. Towns, Livingston, N.J., assignor to C. R. Bard, Inc., Murray Hill, N.J., a corporation of New York Filed Oct. 16, 1963, Ser. No. 316,744 6 Claims. (Cl. 215-47) This invention relates to a test tube closure, and particularly a closure for a culture container, having for an object the provision of such a closure which comprises a top and a pair of depending skirts, the inner one of which is designed and adapted to fit within a plain cylindrical tube and is so formed that, when partly inserted therein, the assembly or unit may be evacuated of air and filled with a sterilizing gas, while the farther insertion causes the closure completely to seal the tube; the other I and outer skirt, which is for manual operation, being spaced from the inner one so as to fit freely about the exterior of the tube and thus avoid preventing the ingress of gas when the tube is being sterilized. The inner skirt is longer than the outer one in order to accommodate the formations necessary for the above mentioned functions, while the length of the outer skirt is merely sufficient for convenient handling when inserting the closure to its partial and complete sealing positions and when removing the closure for repeated use of the assembly or unit.

Another object is the provision of such a dual skirted closure that is fitted functionally to cooperate with a cylindrical tube which is devoid of any structural configuration for coaction with the closure, and thus is equally adapted for use with tubes having either unthickened or beaded open ends.

Another object is the provision of such a closure that is formed with a fiat top of considerable area to permit standing the same on one end with the usual swab sticks that are attached to the top extending upwardly without danger of contamination while preparing the patient for the taking of a culture specimen.

-Another object is to provide the top with a projecting edge that is shaped to prevent rolling of the assembly or unit when placed on its side.

A further object is to provide certain improvements in the form, construction and arrangement of the features or functional elements of the closure whereby the above recited objects, and others inherent in the invention, may be efiiciently attained.

A practical embodiment of the invention is shown in the accompanying drawing, wherein FIG. 1 represents a perspective view of the complete assembly or unit;

FIG. 2 represents, on an enlarged scale, a view looking toward the bottom or open end of the closure;

FIG. 3 represents a similar view looking toward the top of the closure;

FIG. 4 represents, on the same scale, a broken perspective View of the closure alone; and

FIG. 5 represents, on a still larger scale, a sectional view taken on the line V-V of FIG. 2, looking in the direction of the arrows.

In brief summary, this invention contemplates a cylindrical, dual skirted cap, of which the inner skirt is intended to be inserted within the open end of a test tube, and is formed with a pair of circumferential longitudinally spaced beads that are adapted closely to coact with the inner surface of the tube for holding the cap in two positions in one of which there is partial closure of the tube and in the other of which there is complete closure or sealing. The inner bead is interrupted by a longitudinally disposed groove that serves to open communication between the inner body of the tube and the outer bead, while the latter is continuous and serves tightly to seal the tube when in contact with the inner surface thereof. Thus the cap may first be inserted to such an extent that the interrupted head is within the tube while the continuous bead is without. In this first position the tube may be evacuated of air and filled with a sterilizing gas due to the presence of the longitudinal groove. Thereafter, the cap may be farther inserted into the tube to its sealing position in which the outer continuous bead closely contacts the inside of the tube while the inner surface of the top of the cap presses upon the edge of the open end of the tube. The top of the cap is enlarged in area to permit standing on end and its corners project to inhibit rolling when the assembly or unit is on its side. The tube is composed of a transparent plastic, e.g. polystyrene, to allow visual inspection of its contents, while the cap is composed of any suitable plastic that has a slight elastic quality to enable the outer skirt to ride over any enlargement that may exist at the open end of the tube, and is preferably opaque. Within the inner skirt and fixed thereto is a head piece provided with one or more sockets for receiving the end or ends of one or more swab sticks and holding the same in place with their swabbed end or ends in the tube.

Referring now to the drawing, the tube, which is of the usual test tube shape, i.e. with the closed end rounded, is denoted by 1, and the cap as a whole is marked 2. In FIG. 1, the assembled tube and cap are shown as resting on a fiat surface 3, such as a table, to illustrate the nonrolling characteristic above mentioned.

The cap has a relatively enlarged top constituted by a surrounding flange with laterally projecting corners indicated by 4, 4, 4, 4, which eliminate any rolling when the assembly or unit is on its side, as previously described; while the center of the top is depressed, as well shown in FIG. 5, in order to promote sealing contact with the tube edge when the cap is in closed position. I

A cylindrical inner skirt 5 is formed integrally with g and projects downwardly from the cap top 2, and has a pair of circumferential beads on its outer surface which are longitudinally spaced, the outer head 6 being continu ous while the inner one 7 is interrupted or broken by a longitudinal groove 8, the lower end of which is at the inner edge of this skirt and the upper end of which terminates short of the head 6. The purpose and function of this groove is to open communication between the interior of the tube 1 and the space between the beads 6 and 7, which latter are of such size as very snugly to contact the inner surface of the tube 1.

A circumferential series of longitudinally arranged ridges, two of which are marked 9, promote surface contact of this inner skirt 5 iWllh the tube 1 and also strengthen the skint, While the inner edge of the skirt is beveled, as indicated at 10, to facilitate entry of the skirt into the tube.

Within the inner skirt is a centrally disposed head piece 11 that is fixed in place by four radial wings 12, 12, 12., 12, which are preferably integral with the skirt; and in this head piece are formed a pair of sockets 13, 1-3, designed to receive the ends of swab sticks denoted by 14, 14, which, as is customary, are intended to have suitable swabs, marked 15, .15, placed on their free ends for the taking of cultures or specimens from the patient.

The outer skirt of the cap is denoted by 16. It is shorter than the inner skirt, as clearly shown in the drawing, and is adapted loosely to fit the outside of the tube 1, freely sliding over the edge of the tube and leaving a slight space between it and the latter to permit the inflow of gas to the groove 8 when the tube is being sterilized. The exterior of the skirt 16 may be milled as indicated in FIGS. 1 and 4, to aid in the manipulation of the cap.

11 preparation for use, the assembled tube and cap, with the latter inserted only partially and the continuous bead Patented Jan. 5, 1965 6' outside the-tube, as shown in FIG. 5, are placed in a container of cellophane, Mylar, paper or their equivalents, alone or in combination, which is then sealed. This package is enclosed in a chemical retort and subjected to a relatively high vacuum (e.g., 29 inches) to evacuate most of the air, both from the package and from the tube (through the groove fi), A sterilizing gas, such, for instance, as ethylene oxide, is now introduced into the retort, under pressure, the gas penetrating the cellophane container and seterilizing its interior as well as the interior of the tube to Whichit' passes through the groove 3. Thereafter, the package is removed from the retort and Will remain in its sterilized condition for storage or shipment until required for use. It should be added that this condition results from the fact that atmospheric air is not under any pressure tending to force it into the package, while sufficient sterilizing gas will remain in the package even it" it is compressed somewhat in handling. This procedure is well understood and is described, for instance, in United States Patent No. 3,004,681, issued October 17, 1961.

When used, the assembled tube and cap are removed from; the cellophane container, the cap carrying the swab sticks is pulled from the tube and stood upright on the fiat top of the cap. The culture specimen is taken from the patient on one or more swabs, after which the sticks are reinserted into the tube and the cap is forced to its fully closed position with the bead 6 tightly contacting the inner surface of the tube and the underside of the cap pressing on the edge of the tube, zthus completely sealing the latter in condition to be taken to a laboratory for examinaion and testing without danger of contamination.

The use of the tube may be repeated by the insertion of new swab sticks into the cap, and rte-sterilization as above described, to which continued reuse the article is fully amenable.

As the handling and employment of this article have been explained in the foregoing description it is deemed superfluous to recite a substantial repetition, but it may be emphasized that this invention provides an inexpenisve and fully satisfactory product for this important field of use.

It should also be understood that various changes may be made in the structure, material and precise arrangement of the several parts of the closure Without departing from the spirit and scope of the invention, and hence, i do not intend the coverage of the patent to be limited to the details hereinabovedescribed except as the same may be included the claims or be required by disclosure of the prior art.

Vihat I claim is:

1. A closure for a therapeutic test tube designed and adapted particularly as a culture container, said closure comprising a top and a pair of depending axially aligned shirts one within the other, the inner skirt being fitted for insertion Within the tube and having two external circumferential longitudinally spaced beads adapted closely to contact the tube with a sealing fit, the longitudinally inner bead being interrupted while the longitudinally outer one is continuous, together with a longitudinally disposed groove in the exterior of the inner skirt that extends from the extremity thereof through the longitudinally inner bead but terminates short of the longitudinally outer bead.

2. A closure as defined in claim 1, which is adapted to be partially inserted into the tube with the longitudinally inner bead only in sealing contact therewith to per tit evacuation of air from the tube and the filling of the latter with a sterilizing gas.

3. A closure as defined in claim 2, which is also adapted for farther insertion into the tube to complete the sealing thereof by the longitudinally outer bead and the top of the closure.

4. A closure :1 destined in claim 3, in which the outer skirt is slightly elastic to enable it to ride over any enlargement that may exist at the open end of the tube and is spaced sufficiently from the inner skirt loosely to fit the outside of the tube for permitting evacuation of air and ingress of sterilization gas when the closure is partially inserted in the tube.

5. A closure as defined in claim 4, which also includes a head piece fixed within the inner skirt and provided with at least one socket adapted removably to receive and hold a swab stick.

6. A closure as defined in claim 1, in which the top is larger in area than the cross section of the outer skirt and formed with a peripheral edge shaped to prevent rolling of the tube when laid horizontally.

References Cited by the Examiner UNITED STATES PATENTS 2,644,978 7/53 Becker 2l54l 2,721,595 10/55 Nichols 215-47 2,991,902 7/61 Lind 21541 3,005,564 10/61 VJeichselbaum 2l547 3,057,502 10/62 Wood 2l5-47 FOREIGN PATENTS 230,997 5/59 Australia.

FRANKLIN T. GARRETT, Primary Examiner.

Claims (1)

1. A CLOSURE FOR A THERAPEUTIC TEST TUBE DESIGNED AND ADAPTED PARTICULARLY AS A CULTURE CONTAINER, SAID CLOSURE COMPRISING A TOP AND A PAIR OF DEPENDING AXIALLY ALIGNED SKIRTS ONE WITHIN THE OTHER, THE INNER SKIRT BEING FITTED FOR INSERTION WITHIN THE TUBE AND HAVING TWO EXTERNAL CIRCUMFERENTIAL LONGITUDINALLY SPACED BEADS ADAPTED CLOSELY TO CONTACT THE TUBE WITH A SEALING FIT, THE LONGITUDINALLY INNER BEAD BEING INTERRUPTED WHILE THE LONGITUDINALLY OUTER ONE IS CONTINUOUS, TOGETHER WITH A LONGITUDINALLY DISPOSED GROOVE IN THE EXTERIOR OF THE INNER SKIRT THAT EXTENDS FROM THE EXTREMITY THEREOF THROUGH THE LONGITUDINALLY INNER BEAD BUT TERMINATES SHORT OF THE LONGITUDINALLY OUTER BEAD.
US3164279A Test tube closure Expired - Lifetime US3164279A (en)

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Cited By (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3283894A (en) * 1965-02-09 1966-11-08 Tiros Plastics Corp Packaging container
US3330280A (en) * 1964-08-25 1967-07-11 Duo Matic Corp Combination syringe vial and plunger and syringe
US3370588A (en) * 1965-08-18 1968-02-27 Burron Medical Prod Inc Hypodermic needle guard
US3381813A (en) * 1965-09-07 1968-05-07 Pharmaseal Lab Hypodermic needle and protector therefor
US3390759A (en) * 1967-05-25 1968-07-02 Becton Dickinson Co Shield and hub for disposable needle
US3530979A (en) * 1967-02-16 1970-09-29 Gerber Prod Nipple assembly
US3578195A (en) * 1969-01-16 1971-05-11 Lyoflo Stopper Corp Lyoflo-stopper
US3640418A (en) * 1970-04-06 1972-02-08 Dover Molded Products Co Throwaway closure for test tube
US3803915A (en) * 1972-08-21 1974-04-16 J Chaney Food preparing thermometers
US3826396A (en) * 1972-10-31 1974-07-30 Bard Inc C R Double seal closure plug
US3834571A (en) * 1972-11-20 1974-09-10 Warner Lambert Co Container closure for lyophilized products
US3881626A (en) * 1972-11-20 1975-05-06 Warner Lambert Co Container closure for lyophilized products
US3944104A (en) * 1974-11-25 1976-03-16 Consumers Glass Company Limited Threaded wine bottle stopper
US4057168A (en) * 1975-07-07 1977-11-08 Bosshold Barry L Vented test tube top
US4221078A (en) * 1978-09-27 1980-09-09 Latham John F Sealed globular display device
US4720017A (en) * 1982-07-27 1988-01-19 Medical Media Laboratory, Inc. Specimen kits and stopper therefor
US5056681A (en) * 1988-09-28 1991-10-15 Howes James P Prize holding container assemblies
US5203825A (en) * 1991-06-07 1993-04-20 Becton, Dickinson And Company Capillary tube assembly including a vented cap
US20050202119A1 (en) * 2004-03-10 2005-09-15 Donald Ullman Apparatus for enhancing a chewing gum experience
EP2397418A1 (en) * 2010-06-21 2011-12-21 Weener Plastik AG Method for assembling a multi-part closure for containers and multi-part closure
EP2517791A1 (en) * 2011-04-18 2012-10-31 Vibod GmbH Sample tube with improved lid
JP2014515108A (en) * 2011-04-19 2014-06-26 ポーレックス コーポレイション Collection of liquid sample, storage, transportation and delivery device
US20150069008A1 (en) * 2012-05-09 2015-03-12 B. Braun Melsungern Ag Closure cap
US20150164485A1 (en) * 2013-12-12 2015-06-18 ASCLEMED USA, INC. dba EnovaChem Manufacturing Dna medicated collection kits

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2644978A (en) * 1950-06-09 1953-07-14 United Carr Fastener Corp Closure member for tube ends
US2721595A (en) * 1953-11-19 1955-10-25 Celluplastic Corp Ribbed plug type cap for plastic extrusion tube container
US2991902A (en) * 1956-11-27 1961-07-11 Lind Evald Torbjorn Gustav Sealing devices
US3005564A (en) * 1959-10-12 1961-10-24 Biolog Res Inc Laboratory equipment
US3057502A (en) * 1957-04-24 1962-10-09 Permuta Closures Ltd Stoppers

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2644978A (en) * 1950-06-09 1953-07-14 United Carr Fastener Corp Closure member for tube ends
US2721595A (en) * 1953-11-19 1955-10-25 Celluplastic Corp Ribbed plug type cap for plastic extrusion tube container
US2991902A (en) * 1956-11-27 1961-07-11 Lind Evald Torbjorn Gustav Sealing devices
US3057502A (en) * 1957-04-24 1962-10-09 Permuta Closures Ltd Stoppers
US3005564A (en) * 1959-10-12 1961-10-24 Biolog Res Inc Laboratory equipment

Cited By (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3330280A (en) * 1964-08-25 1967-07-11 Duo Matic Corp Combination syringe vial and plunger and syringe
US3283894A (en) * 1965-02-09 1966-11-08 Tiros Plastics Corp Packaging container
US3370588A (en) * 1965-08-18 1968-02-27 Burron Medical Prod Inc Hypodermic needle guard
US3381813A (en) * 1965-09-07 1968-05-07 Pharmaseal Lab Hypodermic needle and protector therefor
US3530979A (en) * 1967-02-16 1970-09-29 Gerber Prod Nipple assembly
US3390759A (en) * 1967-05-25 1968-07-02 Becton Dickinson Co Shield and hub for disposable needle
US3578195A (en) * 1969-01-16 1971-05-11 Lyoflo Stopper Corp Lyoflo-stopper
US3640418A (en) * 1970-04-06 1972-02-08 Dover Molded Products Co Throwaway closure for test tube
US3803915A (en) * 1972-08-21 1974-04-16 J Chaney Food preparing thermometers
US3826396A (en) * 1972-10-31 1974-07-30 Bard Inc C R Double seal closure plug
US3834571A (en) * 1972-11-20 1974-09-10 Warner Lambert Co Container closure for lyophilized products
US3881626A (en) * 1972-11-20 1975-05-06 Warner Lambert Co Container closure for lyophilized products
US3944104A (en) * 1974-11-25 1976-03-16 Consumers Glass Company Limited Threaded wine bottle stopper
US4057168A (en) * 1975-07-07 1977-11-08 Bosshold Barry L Vented test tube top
US4221078A (en) * 1978-09-27 1980-09-09 Latham John F Sealed globular display device
US4720017A (en) * 1982-07-27 1988-01-19 Medical Media Laboratory, Inc. Specimen kits and stopper therefor
US5056681A (en) * 1988-09-28 1991-10-15 Howes James P Prize holding container assemblies
US5203825A (en) * 1991-06-07 1993-04-20 Becton, Dickinson And Company Capillary tube assembly including a vented cap
US20050202119A1 (en) * 2004-03-10 2005-09-15 Donald Ullman Apparatus for enhancing a chewing gum experience
EP2397418A1 (en) * 2010-06-21 2011-12-21 Weener Plastik AG Method for assembling a multi-part closure for containers and multi-part closure
EP2517791A1 (en) * 2011-04-18 2012-10-31 Vibod GmbH Sample tube with improved lid
US8920339B2 (en) 2011-04-19 2014-12-30 Porex Corporation Liquid sampling, storage, transfer and delivery device
US9696241B2 (en) 2011-04-19 2017-07-04 Porex Corporation Liquid sampling, storage, transfer and delivery device
JP2014515108A (en) * 2011-04-19 2014-06-26 ポーレックス コーポレイション Collection of liquid sample, storage, transportation and delivery device
US8852122B2 (en) 2011-04-19 2014-10-07 Porex Corporation Liquid sampling, storage, transfer and delivery device
JP2014534058A (en) * 2011-10-13 2014-12-18 フィボット ゲーエムベーハー Sample tube with improved lid
WO2013053620A1 (en) 2011-10-13 2013-04-18 Vibod Gmbh Sample tube with improved lid
US20150069008A1 (en) * 2012-05-09 2015-03-12 B. Braun Melsungern Ag Closure cap
US9731872B2 (en) * 2012-05-09 2017-08-15 B. Braun Melsungern Ag Closure cap
US20150164485A1 (en) * 2013-12-12 2015-06-18 ASCLEMED USA, INC. dba EnovaChem Manufacturing Dna medicated collection kits

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