US20180011020A9 - Optical density monitor and comparator systems and methods - Google Patents
Optical density monitor and comparator systems and methods Download PDFInfo
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Definitions
- the present disclosure relates generally to systems and methods for determining the absorption coefficient and the optical density of a fluid as they relate to the wavelength of incident radiation.
- ultraviolet light-emitting diodes UV LEDs or the like that emit ultraviolet (UV) radiation or the like are used as sources for irradiating the interior of an integrating chamber that is designed to increase the path length of the radiation through the fluid, thus enhancing the detection limits of the absorption coefficient and the optical density according to Beer's Law.
- integrating spheres or multi-pass cells for quantifying the optical density of a fluid is prolific; however, in most of these applications, the fluid being examined is disposed in a sample container (e.g. a cuvette) and placed inside or against a porthole manufactured in the side of the sphere of cell.
- a radiation source typically a halogen, mercury, or deuterium lamp, is used to irradiate the fluid inside the sample container. Some light is absorbed by the fluid and the remainder is scattered into the interior of the sphere or cell. After being multiply scattered, the remaining light exits the sphere or cell through a porthole and is detected by a spectrometer or the like.
- the path length in this type of measurement is determined by the dimensions of the cuvette, and is not enhanced by multiple scattering inside the sphere or cell.
- This is a common methodology in the field of absorption spectroscopy, where the amount of light absorbed can be correlated to the concentration of a molecule in the fluid.
- attenuation due to light absorption can be separated from attenuation due to light scattering through the integrating sphere or multi-pass cell; however, there is no enhancement of absorption coefficient measurement, as the path length is determined strictly by the cuvette dimensions.
- nucleic acid quantitation is used prolifically to determine the presence and the concentration of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) in a sample.
- DNA deoxyribonucleic acid
- RNA ribonucleic acid
- the Slot-Blot technique can also be used for relatively low sample concentrations, but requires adding a hybridizing agent and relies on luminescence measurement.
- Absorption spectroscopy is a commonly used method whereby the sample absorbance at 260 nm is correlated to the concentration and the sample absorbance at 280 nm. It may also be used to ascertain sample purity or contamination of a protein sample by DNA as compared to the 260 nm absorption. This method is defined by the Beer-Lambert Law, where absorption is a function of the path length of the sample. Because, as described above, the samples of interest are typically in held small cuvettes (i.e. about 1-cm path length) or microplates, they must have sufficient DNA concentrations to allow for measurement, or, be mechanically concentrated prior to sampling (as required for a nanodrop spectrophotometer).
- path length of the sample can be increased, then the level of sensitivity of the measurement can be increased; alternatively, smaller sample volumes can be used for measurement.
- the present disclosure provides an integrating sphere or multi-pass cell (collectively referred to herein as a flow cell) including one or more UV LEDs or the like disposed around the interior periphery thereof.
- the one or more UV LEDs are operable for irradiating the interior of the flow cell, which contains and confines a fluid sample in substantially the entire internal volume of the flow cell, thereby maximizing path length through the fluid sample.
- a detector is also disposed at the interior periphery of the flow cell, such that scattered radiation within the interior of the flow cell impinges on and is detected by the detector.
- the present disclosure provides a system for determining the absorption coefficient and/or the optical density of a fluid, including: an integrating flow cell configured to contain a fluid sample within substantially an entire interior portion thereof; one or more point radiation sources disposed about an interior periphery of the integrating flow cell, wherein the one or more point radiation sources are operable for delivering radiation of a predetermined wavelength to the fluid sample; and one or more radiation detectors operable for detecting radiation within the interior portion of the integrating flow cell; wherein an interior surface of the integrating flow cell is operable for reflecting the radiation delivered to the fluid sample by the one or more point radiation sources; and wherein a path length of the radiation delivered to the fluid sample and reflected is maximized by the fluid sample occupying substantially the entire interior portion of the integrating flow cell.
- the absorption coefficient and/or the optical density of the fluid is determined utilizing the predetermined wavelength of the delivered radiation and a characteristic (e.g. intensity) of the detected radiation.
- the integrating flow cell includes one or more of an integrating cavity, an integrating ellipsoid, an integrating sphere, and a multi-pass cell.
- the one or more point radiation sources include a first point radiation source operable for delivering radiation of a first predetermined wavelength to the fluid sample.
- the one or more point radiation sources further include a second point radiation source operable for delivering radiation of a second predetermined wavelength to the fluid sample.
- the one or more point radiation sources include one or more UV point radiation sources.
- the one or more point radiation sources include a point radiation source operable for delivering radiation having a predetermined wavelength of between about 260 nm and about 280 nm to the fluid sample.
- the interior surface of the integrating flow cell is operable for reflecting the radiation delivered to the fluid sample by the one or more point radiation sources such that a radiation intensity is uniform throughout the interior portion of the integrating flow cell.
- the system also includes an algorithm operable for quantifying a degree of disinfection of the fluid sample using the detected radiation.
- the system includes an algorithm operable for quantifying nucleic acids in the fluid sample using the detected radiation.
- the present disclosure provides a method for determining the absorption coefficient and/or the optical density of a fluid, including: providing an integrating flow cell configured to contain a fluid sample within substantially an entire interior portion thereof; providing one or more point radiation sources disposed about an interior periphery of the integrating flow cell, wherein the one or more point radiation sources are operable for delivering radiation of a predetermined wavelength to the fluid sample; and providing one or more radiation detectors operable for detecting radiation within the interior portion of the integrating flow cell; wherein an interior surface of the integrating flow cell is operable for reflecting the radiation delivered to the fluid sample by the one or more point radiation sources; and wherein a path length of the radiation delivered to the fluid sample and reflected is maximized by the fluid sample occupying substantially the entire interior portion of the integrating flow cell.
- the absorption coefficient and/or the optical density of the fluid is determined utilizing the predetermined wavelength of the delivered radiation and a characteristic (e.g. intensity) of the detected radiation.
- the integrating flow cell includes one or more of an integrating cavity, an integrating ellipsoid, an integrating sphere, and a multi-pass cell.
- the one or more point radiation sources include a first point radiation source operable for delivering radiation of a first predetermined wavelength to the fluid sample.
- the one or more point radiation sources further include a second point radiation source operable for delivering radiation of a second predetermined wavelength to the fluid sample.
- the one or more point radiation sources include one or more UV point radiation sources.
- the one or more point radiation sources include a point radiation source operable for delivering radiation having a predetermined wavelength of between about 260 nm and about 280 nm to the fluid sample.
- the interior surface of the integrating flow cell is operable for reflecting the radiation delivered to the fluid sample by the one or more point radiation sources such that a radiation intensity is uniform throughout the interior portion of the integrating flow cell.
- the method also includes providing an algorithm operable for quantifying a degree of disinfection of the fluid sample using the detected radiation.
- the method includes providing an algorithm operable for quantifying nucleic acids in the fluid sample using the detected radiation.
- FIG. 1 is a schematic diagram illustrating one exemplary embodiment of the optical density monitor and comparator system of the present disclosure, containing a fluid sample to be quantified;
- FIG. 2 is a schematic diagram illustrating one exemplary embodiment of the optical density monitor and comparator system of the present disclosure, containing a fluid sample and DNA/RNA or the like to be quantified;
- FIG. 3 is a plot illustrating photodiode response in a 4-inch integrating sphere with a 255-nm LED source to various DNA dilutions
- FIG. 4 is a plot illustrating photodiode response positioned in the side of a Beta Pearl reactor which was filled with air, distilled water, and distilled water combined with varying amounts of instant coffee to adjust the UV transmittance of the water.
- the present disclosure provides an integrating sphere or multi-pass cell (collectively referred to herein as a flow cell 10 ) including one or more UV LEDs or the like 12 disposed around and/or through the interior periphery 14 thereof.
- the one or more UV LEDs 12 are operable for irradiating the interior 16 of the flow cell 10 , which contains and confines a fluid sample 18 in substantially the entire internal volume 16 of the flow cell 10 , thereby maximizing path length 20 through the fluid sample 18 .
- the path length 20 substantially coincides with the entire internal diameter 22 of the flow cell 10 .
- One or more detectors 24 are also disposed at the interior periphery 14 of the flow cell 10 , such that scattered radiation 26 within the interior 16 of the flow cell 10 impinges on and is detected by the one or more detectors 24 .
- the enhanced pathlength offered by integration spheres provides advantages in sensitivity.
- a 10-cm diameter integrating sphere results in a 250-cm pathlength, which is 250 ⁇ that of a standard 1-cm cuvette.
- the increased pathlength inside the integrating sphere is a result of multiple reflections of the incoming radiation from the walls of the sphere which are typically made from a Lambertian scattering material. Each time the incoming radiation is reflected there is some attenuation of the signal which is affected by the reflectivity of the wall of the sphere as well as the absorption coefficient of the medium inside the sphere.
- a good approximation of the pathlength is determined by setting the Beer-Lambert law equal to the integrating sphere multiplier, M, which describes the increase in radiance in an integrating sphere due to multiple reflections.
- f is the port hole fraction in the sphere and ⁇ is the reflectivity of the sphere wall and ⁇ and ⁇ 0 are the internal radiance and initial radiance in the sphere, respectively.
- ⁇ ⁇ 0 ⁇ - ⁇ ⁇ ⁇ z
- ⁇ is the absorption coefficient and z is the pathlength and ⁇ and ⁇ 0 are the radiance with and without the presence of absorption, respectively.
- the flow cell 10 takes the form of an integrating sphere or the like. Although the integrating sphere configuration is discussed at length, other configurations may also be utilized. The key consideration is that photons are repeatedly reflected within the flow cell 10 and that a uniform radiation field is formed with low-intensity radiation sources.
- the flow cell 10 should have substantially curved and concave opposing interior surfaces, should not have internal corners, and every point on the interior surface of the flow cell 10 should be “visible” from every other point on the interior surface. Ovoids, ellipsoids, cubes with rounded corners, etc. all fit these criteria.
- the flow cell 10 is made of plastic or the like for ease of manufacturing, and, in such cases where the material is not a good Lambertian scatterer, the interior surfaces thereof are coated with a Lambertian scattering material.
- the flow cell 10 is made of a metallic or other reflective or coated reflective material, such as aluminum, stainless steel, copper, etc., which may be anodized or otherwise coated with organic polymer, silicone, inorganic oxide, etc.
- the flow cell 10 is scalable and may have any suitable dimensions, on the order of millimeters to meters, for example.
- the flow cell 10 includes at least an inlet port (not illustrated) and an outlet port (not illustrated) manufactured into it that provides for the flow of the fluid sample 18 (i.e. a liquid or a gas) from the inlet port to the outlet port.
- the inlet port and the outlet port may consist of the sample port. It will be readily apparent to those of ordinary skill in the art that multiple inlet ports and/or multiple outlet ports may also be utilized.
- one or more point radiation sources 12 such as one or more UV optical sources, one or more deep-UV optical sources, one or more semiconductor optical sources, and/or one or more light-emitting diode (LED) optical sources, are disposed within or partially or wholly through one or more ports (not illustrated) manufactured through the flow cell 10 , optionally at symmetrically-disposed positions.
- Point radiation sources refer to small, roughly symmetrical radiation sources, as compared to the other dimensions of the system.
- one exemplary application of the present invention involves measuring the optical density of a water influent or effluent to a disinfection reactor or the like.
- the optical density of water in the UV region is important for determining the efficacy of water disinfection reactors that use UV radiation to inactivate organisms in the water. This practice is commonly referred to as measuring the UV transmission (UVT) of the water.
- UVT UV transmission
- one LED is used at 260 nm, for example, the typical wavelength used for water disinfection.
- Another exemplary application of the present invention involves measuring nucleic acid concentrations in a sample and quantifying the ratio of nucleic acids to protein in solution.
- the present invention is significantly different from the current methods of sample measurement in that it allows the sample to fill the entirety of the interior of the integrating sphere 10 , making the sphere the effective sample holder. This enables a higher level of sensitivity in the measurement because it maximizes the path length 20 for both the irradiating source 12 and the sample 18 in a compact design.
- the sphere 10 operates on the principle of multiple reflections, it is extremely sensitive to absorbing molecules.
- Two LED optical sources 12 are used in this implementation, for example, one at 260 nm, the peak of DNA absorbance, and one at 280 nm, the peak of protein absorbance.
- a detector 24 on a porthole of the integrating sphere 10 measures a specific intensity at wavelength-1 and wavelength-2 (typically with 260 nm and 280 nm LED sources irradiating the interior 16 of the sphere 10 , for example).
- the intensity at each wavelength drops with the addition of the sample 18 inside the sphere 10 (right-hand side) and the sensitivity to the sample absorption is increased due to the long path length 26 provided by the integrating sphere 10 .
Abstract
Description
- The present patent application/patent is a continuation-in-part of co-pending U.S. patent application Ser. No. 13/163,055, filed on Jun. 17, 2011, and entitled “SYSTEMS AND METHODS FOR PERFORMING THE BACTERIAL DISINFECTION OF A FLUID USING POINT RADIATION SOURCES,” which claims the benefit of priority of Patent Cooperation Treaty (PCT) Patent Application No. PCT/US09/68765, filed on Dec. 18, 2009, and entitled “SYSTEMS AND METHODS FOR PERFORMING THE BACTERIAL DISINFECTION OF A FLUID USING POINT RADIATION SOURCES,” which claims the benefit of priority of U.S. Provisional Patent Application No. 61/139,022, filed on Dec. 19, 2008, and entitled “BACTERIAL DISINFECTION UNIT,” the contents of all of which are incorporated in full by reference herein. The present patent application/patent also claims the benefit of priority of co-pending U.S. Provisional Patent Application No. 61/820,204, filed on May 7, 2013, and entitled “OPTICAL DENSITY MONITOR AND COMPARATOR,” the contents of which are incorporated in full by reference herein.
- The U.S. Government may have certain rights in the present disclosure pursuant to Award Nos. 0740524 and 0848759 by the National Science Foundation (NSF).
- The present disclosure relates generally to systems and methods for determining the absorption coefficient and the optical density of a fluid as they relate to the wavelength of incident radiation. Specifically, ultraviolet light-emitting diodes (UV LEDs) or the like that emit ultraviolet (UV) radiation or the like are used as sources for irradiating the interior of an integrating chamber that is designed to increase the path length of the radiation through the fluid, thus enhancing the detection limits of the absorption coefficient and the optical density according to Beer's Law.
- The use of integrating spheres or multi-pass cells for quantifying the optical density of a fluid is prolific; however, in most of these applications, the fluid being examined is disposed in a sample container (e.g. a cuvette) and placed inside or against a porthole manufactured in the side of the sphere of cell. A radiation source, typically a halogen, mercury, or deuterium lamp, is used to irradiate the fluid inside the sample container. Some light is absorbed by the fluid and the remainder is scattered into the interior of the sphere or cell. After being multiply scattered, the remaining light exits the sphere or cell through a porthole and is detected by a spectrometer or the like. The path length in this type of measurement is determined by the dimensions of the cuvette, and is not enhanced by multiple scattering inside the sphere or cell. This is a common methodology in the field of absorption spectroscopy, where the amount of light absorbed can be correlated to the concentration of a molecule in the fluid. In this methodology, attenuation due to light absorption can be separated from attenuation due to light scattering through the integrating sphere or multi-pass cell; however, there is no enhancement of absorption coefficient measurement, as the path length is determined strictly by the cuvette dimensions. Thus, what are still needed in the art are improved systems and methods for determining the absorption coefficient and the optical density of a fluid as they relate to the wavelength of incident radiation.
- Further, nucleic acid quantitation is used prolifically to determine the presence and the concentration of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) in a sample. There are several methodologies used to measure such concentrations. When relatively low concentrations of DNA are present, fluorescent dyes are used that bind to the nucleic acid and the resulting fluorescent intensity is compared to control samples. This method can be more time consuming than other methods, but is more accurate at relatively low sample concentrations. The Slot-Blot technique can also be used for relatively low sample concentrations, but requires adding a hybridizing agent and relies on luminescence measurement. Absorption spectroscopy is a commonly used method whereby the sample absorbance at 260 nm is correlated to the concentration and the sample absorbance at 280 nm. It may also be used to ascertain sample purity or contamination of a protein sample by DNA as compared to the 260 nm absorption. This method is defined by the Beer-Lambert Law, where absorption is a function of the path length of the sample. Because, as described above, the samples of interest are typically in held small cuvettes (i.e. about 1-cm path length) or microplates, they must have sufficient DNA concentrations to allow for measurement, or, be mechanically concentrated prior to sampling (as required for a nanodrop spectrophotometer). If the path length of the sample can be increased, then the level of sensitivity of the measurement can be increased; alternatively, smaller sample volumes can be used for measurement. Thus, what are still needed in the art are improved systems and methods for determining the presence and the concentration of DNA and RNA in a sample by exploiting increased path length.
- In various exemplary embodiments, the present disclosure provides an integrating sphere or multi-pass cell (collectively referred to herein as a flow cell) including one or more UV LEDs or the like disposed around the interior periphery thereof. The one or more UV LEDs are operable for irradiating the interior of the flow cell, which contains and confines a fluid sample in substantially the entire internal volume of the flow cell, thereby maximizing path length through the fluid sample. A detector is also disposed at the interior periphery of the flow cell, such that scattered radiation within the interior of the flow cell impinges on and is detected by the detector.
- In one exemplary embodiment, the present disclosure provides a system for determining the absorption coefficient and/or the optical density of a fluid, including: an integrating flow cell configured to contain a fluid sample within substantially an entire interior portion thereof; one or more point radiation sources disposed about an interior periphery of the integrating flow cell, wherein the one or more point radiation sources are operable for delivering radiation of a predetermined wavelength to the fluid sample; and one or more radiation detectors operable for detecting radiation within the interior portion of the integrating flow cell; wherein an interior surface of the integrating flow cell is operable for reflecting the radiation delivered to the fluid sample by the one or more point radiation sources; and wherein a path length of the radiation delivered to the fluid sample and reflected is maximized by the fluid sample occupying substantially the entire interior portion of the integrating flow cell. The absorption coefficient and/or the optical density of the fluid is determined utilizing the predetermined wavelength of the delivered radiation and a characteristic (e.g. intensity) of the detected radiation. The integrating flow cell includes one or more of an integrating cavity, an integrating ellipsoid, an integrating sphere, and a multi-pass cell. Preferably, the one or more point radiation sources include a first point radiation source operable for delivering radiation of a first predetermined wavelength to the fluid sample. Optionally, the one or more point radiation sources further include a second point radiation source operable for delivering radiation of a second predetermined wavelength to the fluid sample. Optionally, the one or more point radiation sources include one or more UV point radiation sources. Optionally, the one or more point radiation sources include a point radiation source operable for delivering radiation having a predetermined wavelength of between about 260 nm and about 280 nm to the fluid sample. The interior surface of the integrating flow cell is operable for reflecting the radiation delivered to the fluid sample by the one or more point radiation sources such that a radiation intensity is uniform throughout the interior portion of the integrating flow cell. Optionally, the system also includes an algorithm operable for quantifying a degree of disinfection of the fluid sample using the detected radiation. Alternatively, the system includes an algorithm operable for quantifying nucleic acids in the fluid sample using the detected radiation.
- In another exemplary embodiment, the present disclosure provides a method for determining the absorption coefficient and/or the optical density of a fluid, including: providing an integrating flow cell configured to contain a fluid sample within substantially an entire interior portion thereof; providing one or more point radiation sources disposed about an interior periphery of the integrating flow cell, wherein the one or more point radiation sources are operable for delivering radiation of a predetermined wavelength to the fluid sample; and providing one or more radiation detectors operable for detecting radiation within the interior portion of the integrating flow cell; wherein an interior surface of the integrating flow cell is operable for reflecting the radiation delivered to the fluid sample by the one or more point radiation sources; and wherein a path length of the radiation delivered to the fluid sample and reflected is maximized by the fluid sample occupying substantially the entire interior portion of the integrating flow cell. The absorption coefficient and/or the optical density of the fluid is determined utilizing the predetermined wavelength of the delivered radiation and a characteristic (e.g. intensity) of the detected radiation. The integrating flow cell includes one or more of an integrating cavity, an integrating ellipsoid, an integrating sphere, and a multi-pass cell. Preferably, the one or more point radiation sources include a first point radiation source operable for delivering radiation of a first predetermined wavelength to the fluid sample. Optionally, the one or more point radiation sources further include a second point radiation source operable for delivering radiation of a second predetermined wavelength to the fluid sample. Optionally, the one or more point radiation sources include one or more UV point radiation sources. Optionally, the one or more point radiation sources include a point radiation source operable for delivering radiation having a predetermined wavelength of between about 260 nm and about 280 nm to the fluid sample. The interior surface of the integrating flow cell is operable for reflecting the radiation delivered to the fluid sample by the one or more point radiation sources such that a radiation intensity is uniform throughout the interior portion of the integrating flow cell. Optionally, the method also includes providing an algorithm operable for quantifying a degree of disinfection of the fluid sample using the detected radiation. Alternatively, the method includes providing an algorithm operable for quantifying nucleic acids in the fluid sample using the detected radiation.
- The present disclosure is illustrated and described herein with reference to the various drawings, in which like reference numbers are used to denote like system components/method steps, as appropriate, and in which:
-
FIG. 1 is a schematic diagram illustrating one exemplary embodiment of the optical density monitor and comparator system of the present disclosure, containing a fluid sample to be quantified; -
FIG. 2 is a schematic diagram illustrating one exemplary embodiment of the optical density monitor and comparator system of the present disclosure, containing a fluid sample and DNA/RNA or the like to be quantified; -
FIG. 3 is a plot illustrating photodiode response in a 4-inch integrating sphere with a 255-nm LED source to various DNA dilutions; and -
FIG. 4 is a plot illustrating photodiode response positioned in the side of a Beta Pearl reactor which was filled with air, distilled water, and distilled water combined with varying amounts of instant coffee to adjust the UV transmittance of the water. - Referring now specifically to
FIG. 1 , again, in one exemplary embodiment, the present disclosure provides an integrating sphere or multi-pass cell (collectively referred to herein as a flow cell 10) including one or more UV LEDs or the like 12 disposed around and/or through theinterior periphery 14 thereof. The one ormore UV LEDs 12 are operable for irradiating theinterior 16 of theflow cell 10, which contains and confines afluid sample 18 in substantially the entireinternal volume 16 of theflow cell 10, thereby maximizingpath length 20 through thefluid sample 18. Thepath length 20 substantially coincides with the entireinternal diameter 22 of theflow cell 10. One ormore detectors 24 are also disposed at theinterior periphery 14 of theflow cell 10, such that scatteredradiation 26 within theinterior 16 of theflow cell 10 impinges on and is detected by the one ormore detectors 24. - The enhanced pathlength offered by integration spheres provides advantages in sensitivity. A 10-cm diameter integrating sphere results in a 250-cm pathlength, which is 250× that of a standard 1-cm cuvette. The increased pathlength inside the integrating sphere is a result of multiple reflections of the incoming radiation from the walls of the sphere which are typically made from a Lambertian scattering material. Each time the incoming radiation is reflected there is some attenuation of the signal which is affected by the reflectivity of the wall of the sphere as well as the absorption coefficient of the medium inside the sphere. A good approximation of the pathlength is determined by setting the Beer-Lambert law equal to the integrating sphere multiplier, M, which describes the increase in radiance in an integrating sphere due to multiple reflections.
-
- Where f is the port hole fraction in the sphere and ρ is the reflectivity of the sphere wall and φ and φ0 are the internal radiance and initial radiance in the sphere, respectively.
-
- Where α is the absorption coefficient and z is the pathlength and φ and φ0 are the radiance with and without the presence of absorption, respectively.
- The resulting effective pathlength, z, in an integrating sphere is defined by the following equation, which has been shown to have good agreement with experimental measurements:
-
- This effective increase in pathlength enables a higher level of sensitivity in the measurement because it maximizes the pathlength for both the irradiating source and the sample in a compact design. Because the sphere operates on the principle of multiple reflections, it is extremely sensitive to absorbing molecules.
- One
exemplary flow cell 10 is described in U.S. Patent Application Publication No. 2012/0318749 (commonly assigned). Theflow cell 10 takes the form of an integrating sphere or the like. Although the integrating sphere configuration is discussed at length, other configurations may also be utilized. The key consideration is that photons are repeatedly reflected within theflow cell 10 and that a uniform radiation field is formed with low-intensity radiation sources. Theflow cell 10 should have substantially curved and concave opposing interior surfaces, should not have internal corners, and every point on the interior surface of theflow cell 10 should be “visible” from every other point on the interior surface. Ovoids, ellipsoids, cubes with rounded corners, etc. all fit these criteria. Theflow cell 10 is made of plastic or the like for ease of manufacturing, and, in such cases where the material is not a good Lambertian scatterer, the interior surfaces thereof are coated with a Lambertian scattering material. Alternatively, theflow cell 10 is made of a metallic or other reflective or coated reflective material, such as aluminum, stainless steel, copper, etc., which may be anodized or otherwise coated with organic polymer, silicone, inorganic oxide, etc. Theflow cell 10 is scalable and may have any suitable dimensions, on the order of millimeters to meters, for example. - The
flow cell 10 includes at least an inlet port (not illustrated) and an outlet port (not illustrated) manufactured into it that provides for the flow of the fluid sample 18 (i.e. a liquid or a gas) from the inlet port to the outlet port. Alternatively, the inlet port and the outlet port may consist of the sample port. It will be readily apparent to those of ordinary skill in the art that multiple inlet ports and/or multiple outlet ports may also be utilized. - In the nominal design, one or more
point radiation sources 12, such as one or more UV optical sources, one or more deep-UV optical sources, one or more semiconductor optical sources, and/or one or more light-emitting diode (LED) optical sources, are disposed within or partially or wholly through one or more ports (not illustrated) manufactured through theflow cell 10, optionally at symmetrically-disposed positions. “Point radiation sources” as used herein, refer to small, roughly symmetrical radiation sources, as compared to the other dimensions of the system. - Referring again specifically to
FIG. 1 , one exemplary application of the present invention involves measuring the optical density of a water influent or effluent to a disinfection reactor or the like. The optical density of water in the UV region is important for determining the efficacy of water disinfection reactors that use UV radiation to inactivate organisms in the water. This practice is commonly referred to as measuring the UV transmission (UVT) of the water. In this implementation, one LED is used at 260 nm, for example, the typical wavelength used for water disinfection. - Another exemplary application of the present invention involves measuring nucleic acid concentrations in a sample and quantifying the ratio of nucleic acids to protein in solution. The present invention is significantly different from the current methods of sample measurement in that it allows the sample to fill the entirety of the interior of the integrating
sphere 10, making the sphere the effective sample holder. This enables a higher level of sensitivity in the measurement because it maximizes thepath length 20 for both the irradiatingsource 12 and thesample 18 in a compact design. Because thesphere 10 operates on the principle of multiple reflections, it is extremely sensitive to absorbing molecules. Two LEDoptical sources 12 are used in this implementation, for example, one at 260 nm, the peak of DNA absorbance, and one at 280 nm, the peak of protein absorbance. - Referring now specifically to
FIG. 2 , the system is used for such nucleic acid quantitation. On the left hand side adetector 24 on a porthole of the integratingsphere 10 measures a specific intensity at wavelength-1 and wavelength-2 (typically with 260 nm and 280 nm LED sources irradiating the interior 16 of thesphere 10, for example). The intensity at each wavelength drops with the addition of thesample 18 inside the sphere 10 (right-hand side) and the sensitivity to the sample absorption is increased due to thelong path length 26 provided by the integratingsphere 10. - By increasing the absorption pathlength of a sample, the level of sensitivity of the measurement will also be increased; alternately, smaller sample volumes or more dilute samples can be used for measurement. This effect was seen in preliminary data taken using a custom four inch diameter integrating sphere made of virgin PTFE with a 96% surface reflectance in the ultraviolet wavelength range. The sphere was filled with deionized water and a 10 mW, 255 nm LED was used as the input radiation source. A SiC photodiode monitored the flux inside the integrating sphere. Herring sperm was inoculated in the integrating sphere at increasingly less dilute samples until an absorption response, seen as a drop in the photodiode current, was measured. As can be seen in
FIG. 3 , a response was measured at a DNA dilution of 1.58 pg/μL. Also, as less dilute samples were inoculated into the sphere the drop in photocurrent increased as was expected. The preliminary data showed a response at 1.58 pg/μL which is 1000× lower than other absorbance based nucleic acid quantitation systems. - The application of using the integrating sphere as an optical density monitor was also explored experimentally. Inside the sphere increasingly higher levels of coffee were added to water to adjust the UVT of the water. The photodiode response of a photodiode situated into the side of the sphere was measured and compared to 1-cm cuvette readings of the same liquid measured using a standard spectrophotometer (see
FIG. 4 ). Large changes in the photodiode response were observed for very small changes in liquid UVT, indicating the sphere could be a very sensitive measurement tool for performing absorption based measurements on liquids contained in the sphere interior. - Although the present disclosure is illustrated and described herein with reference to preferred embodiments and specific examples thereof, it will be readily apparent to those of ordinary skill in the art that other embodiments and examples may perform similar functions and/or achieve like results. All such equivalent embodiments and examples are within the spirit and scope of the present disclosure, are contemplated thereby, and are intended to be covered by the following claims.
Claims (24)
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11365134B2 (en) | 2019-07-31 | 2022-06-21 | Access Business Group International Llc | Water treatment system |
Families Citing this family (51)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102281933A (en) | 2008-12-19 | 2011-12-14 | 北卡罗来纳大学夏洛特分校 | Systems and methods for performing the bacterial disinfection of a fluid using point radiation sources |
US9168321B2 (en) | 2011-11-02 | 2015-10-27 | Syddansk Universitet | Toroidal-shaped treatment device for disinfecting a fluid such as air or water |
US11000622B2 (en) | 2012-07-27 | 2021-05-11 | Aeroclean Technologies, Llc | UV sterilization apparatus, system, and method for forced-air patient heating systems |
US8900519B2 (en) * | 2012-07-27 | 2014-12-02 | Mark D. Krosney | Air sterilization and disinfection apparatus and method |
CN102895687B (en) * | 2012-10-26 | 2014-12-24 | 昇瑞光电科技(上海)有限公司 | Efficient deep ultraviolet ray sterilizing device |
JP5812970B2 (en) * | 2012-11-19 | 2015-11-17 | 株式会社トクヤマ | Air purifier |
US11338048B2 (en) | 2012-12-11 | 2022-05-24 | Aquisense Technologies Llc | Apparatus for irradiation |
US10294124B2 (en) | 2013-01-24 | 2019-05-21 | Atlantium Technologies Ltd. | Method and apparatus for liquid disinfection by light emitted from light emitting diodes |
CA2905257C (en) * | 2013-03-15 | 2022-03-15 | Atlantium Technologies Ltd. | System and method for inactivation of infectious pancreatic necrosis virus (ipnv) using optimized ultraviolet (uv) light |
US10351898B2 (en) * | 2013-05-28 | 2019-07-16 | Ramot At Tel-Aviv University Ltd. | Detection of hydroxymethylcytosine bases |
CN103951008B (en) * | 2014-04-09 | 2019-05-10 | 青岛云路新能源科技有限公司 | Deep ultraviolet LED sterilizing unit |
DE102015108773A1 (en) * | 2014-06-03 | 2015-12-03 | Sensor Electronic Technology, Inc. | Ultraviolet-transparent cladding |
GB201420231D0 (en) * | 2014-11-13 | 2014-12-31 | Greenthread Ltd | Apparatus and method for water treatment |
US9827340B2 (en) * | 2014-12-24 | 2017-11-28 | Maxim Integrated Products, Inc. | Mobile UV sterilization device and method |
GB201502194D0 (en) * | 2015-02-10 | 2015-03-25 | Univ St Andrews | Scattered light integrating collector |
US10180248B2 (en) | 2015-09-02 | 2019-01-15 | ProPhotonix Limited | LED lamp with sensing capabilities |
WO2017070359A1 (en) * | 2015-10-23 | 2017-04-27 | Cleanco Bioscience Group, Llc | Uv sterilization apparatus, system, and method for forced-air patient heating systems |
JP6893397B2 (en) * | 2016-06-22 | 2021-06-23 | 美浜株式会社 | Sterilization equipment |
JP6419760B2 (en) * | 2016-08-30 | 2018-11-07 | 日機装株式会社 | Ultraviolet light sterilizer |
CN106277114A (en) * | 2016-09-30 | 2017-01-04 | 圆融健康科技(深圳)有限公司 | Circulating water disinfection equipment |
US11312642B2 (en) | 2017-03-31 | 2022-04-26 | Industrial Technology Research Institute | Fluid sterilizing device |
CN115340145A (en) | 2017-08-11 | 2022-11-15 | 埃奎森斯技术有限责任公司 | Irradiation device and method |
JP2020532401A (en) | 2017-08-31 | 2020-11-12 | イーディーエイチエム ホールディングス エルエルシー | Air treatment system and method |
JP6978341B2 (en) * | 2018-02-20 | 2021-12-08 | スタンレー電気株式会社 | Fluid sterilizer |
US10793454B1 (en) | 2018-02-22 | 2020-10-06 | United States Of America As Represented By The Secretary Of The Air Force | Water treatment system with ultraviolet LEDs and photo-catalysts |
CN110316786A (en) * | 2018-03-30 | 2019-10-11 | 旭化成株式会社 | Ultraviolet lamp |
US11464885B2 (en) | 2018-04-20 | 2022-10-11 | Asahi Kasei Kabushiki Kaisha | Ultraviolet light irradiation device |
EP3583958B1 (en) | 2018-04-20 | 2021-03-31 | Asahi Kasei Kabushiki Kaisha | Ultraviolet light irradiation device |
DE102018124504A1 (en) * | 2018-10-04 | 2020-04-09 | Hytecon Ag | Device for a device for disinfecting a fluid and device |
CN109574130A (en) * | 2018-12-19 | 2019-04-05 | 青岛杰生电气有限公司 | Overflow-type ultraviolet radiation for sterilizing and disinfecting unit |
US20220088240A1 (en) * | 2019-02-08 | 2022-03-24 | W. L. Gore & Associates, Inc. | Ultraviolet light disinfecting systems |
IL294517A (en) | 2020-01-22 | 2022-09-01 | Strauss Water Ltd | Liquid disinfecting module |
US10894726B1 (en) | 2020-02-12 | 2021-01-19 | Aquisense Technologies, Llc | Water disinfecting module, systems and methods |
US11850336B2 (en) | 2020-05-22 | 2023-12-26 | Molekule Group, Inc. | UV sterilization apparatus, system, and method for aircraft air systems |
RU2729292C1 (en) * | 2020-05-29 | 2020-08-05 | Валерий Владимирович Крюков | Individual and mobile biological protection devices by irradiating flowing air with ultraviolet radiation |
DE202020103352U1 (en) * | 2020-06-10 | 2021-09-16 | BÄRO GmbH & Co. KG | Air purification device, air conditioning system, ventilation or air conditioning system, vehicle and use |
WO2022049048A1 (en) * | 2020-09-03 | 2022-03-10 | Signify Holding B.V. | A light emitting device for disinfection of objects |
DE102021003829A1 (en) | 2020-09-06 | 2022-04-21 | Kastriot Merlaku | Vaccine Generator |
KR102285032B1 (en) * | 2020-09-10 | 2021-08-04 | 김영준 | Sterilization Device Using Laser |
US20220105227A1 (en) * | 2020-10-02 | 2022-04-07 | Dynamis Energy, Llc | Dual chamber ultra-violet led device for use with face masks to disinfect end-user's inhaled and exhaled air |
US11779675B2 (en) | 2020-10-19 | 2023-10-10 | Molekule Group, Inc. | Air sterilization insert for heating, ventilation, and air conditioning (HVAC) systems |
WO2022090901A1 (en) * | 2020-10-30 | 2022-05-05 | 3M Innovative Properties Company | Ultraviolet c (uv-c) light reflector including fluoropolymer films |
CN112499721B (en) * | 2020-11-10 | 2022-08-16 | 佛山科学技术学院 | Water purification reactor with cylindrical deep ultraviolet disinfection radiation source |
CN112499722A (en) * | 2020-11-10 | 2021-03-16 | 佛山科学技术学院 | Annular structure ultraviolet disinfection reactor |
CN112551637A (en) * | 2020-11-11 | 2021-03-26 | 佛山科学技术学院 | Annular double-channel deep ultraviolet water purifier with rectifying device |
WO2022103640A2 (en) * | 2020-11-12 | 2022-05-19 | Wayne State University | Personal ultraviolet respiratory germ elimination machine (pur gem) |
CN112413796A (en) * | 2020-11-25 | 2021-02-26 | 北京新风航天装备有限公司 | Sterilizing equipment for air purification based on ultraviolet pulse laser |
IT202000030899A1 (en) * | 2020-12-15 | 2022-06-15 | Istituto Naz Di Astrofisica | DEVICE FOR THE DISINFECTION OF A FLUID FLOW IN A CONDUIT USING UV-C RADIATIONS |
KR102632871B1 (en) * | 2021-01-14 | 2024-02-05 | (주)엔디에스 | High Efficiency UV Sterilization Device Using Reflector |
EP4104868A1 (en) | 2021-06-17 | 2022-12-21 | Volvo Truck Corporation | A disinfection system for a motorized vehicle |
IT202100028718A1 (en) * | 2021-11-11 | 2023-05-11 | Dario Russo | SANITIZATION APPARATUS |
Family Cites Families (44)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH063780Y2 (en) * | 1988-12-09 | 1994-02-02 | 三菱重工業株式会社 | Container sterilizer |
US5216251A (en) * | 1991-10-18 | 1993-06-01 | Matschke Arthur L | Apparatus and method for a bio-conditioning germicidal dryer |
WO1993012488A1 (en) | 1991-12-13 | 1993-06-24 | White Leonard R | Measurement analysis software system and method |
US5251004A (en) * | 1992-03-13 | 1993-10-05 | Pdt Systems, Inc. | Integrating sphere power meter |
US5874741A (en) * | 1995-10-03 | 1999-02-23 | Matschke; Arthur L. | Apparatus for germicidal cleansing of water |
GB9611698D0 (en) | 1996-06-05 | 1996-08-07 | Iatros Ltd | Fluid processing |
US6042720A (en) | 1996-12-19 | 2000-03-28 | Motorola, Inc. | Apparatus for storing and disinfecting a fluid |
US6118134A (en) * | 1997-09-02 | 2000-09-12 | Justak; John F. | Optical mass gauge sensor having an energy per unit area of illumination detection |
FI106424B (en) | 1998-02-13 | 2001-01-31 | Nokia Networks Oy | Update of routing area in a packet radio network |
US6172387B1 (en) | 1998-05-04 | 2001-01-09 | Micron Technology, Inc. | Semiconductor interconnection structure and method |
US6514405B1 (en) | 1999-08-12 | 2003-02-04 | Eric L. Lifschitz | Portable water purifier with ultraviolet light source |
US6429583B1 (en) | 1998-11-30 | 2002-08-06 | General Electric Company | Light emitting device with ba2mgsi2o7:eu2+, ba2sio4:eu2+, or (srxcay ba1-x-y)(a1zga1-z)2sr:eu2+phosphors |
IL129564A (en) | 1999-04-23 | 2004-06-20 | Atlantium Lasers Ltd | Method for disinfecting and purifying liquids and gases and a device to use therein |
US6494617B1 (en) | 1999-04-30 | 2002-12-17 | General Electric Company | Status detection apparatus and method for fluid-filled electrical equipment |
US6446027B1 (en) | 1999-09-17 | 2002-09-03 | General Electric Company | Intelligent analysis system and method for fluid-filled electrical equipment |
US6766097B2 (en) | 2000-11-28 | 2004-07-20 | Remote Light, Inc. | UV portal-based appliances and containers |
US6469308B1 (en) | 2001-05-01 | 2002-10-22 | Ryan M. Reed | Ultraviolet radiated water treatment tank |
KR100891031B1 (en) | 2001-11-02 | 2009-03-31 | 허니웰 인터내셔널 인코포레이티드 | Ultraviolet disinfecting apparatus |
US6841406B2 (en) | 2001-11-06 | 2005-01-11 | Edward Brittain Stokes | Methods and apparatus for a semiconductor device |
US20040039242A1 (en) * | 2002-04-02 | 2004-02-26 | Seedling Enterprises, Llc | Apparatus and methods using visible light for debilitating and/or killing microorganisms within the body |
US7118852B2 (en) * | 2002-04-11 | 2006-10-10 | Throwleigh Technologies, L.L.C. | Methods and apparatus for decontaminating fluids |
US7125485B2 (en) | 2002-05-14 | 2006-10-24 | Jamel Hanbli | Water cooler/water purification system |
US20040076544A1 (en) | 2002-10-09 | 2004-04-22 | Hung Dao | Method and apparatus for scanning and sterilizing mail received at a drop box |
US6787782B1 (en) | 2003-04-23 | 2004-09-07 | B/E Aerospace, Inc. | Ultraviolet-light vehicle air cleaning system |
WO2004103443A1 (en) | 2003-05-22 | 2004-12-02 | Beijing Jingjing Medical Equipment Co., Ltd. | Method for inactivating virus in circular blood and its applictions in treating viral diseases |
IL157229A (en) * | 2003-08-04 | 2006-08-20 | Zamir Tribelsky | Method for energy coupling especially useful for disinfecting and various systems using it |
EP1704596A2 (en) | 2003-09-05 | 2006-09-27 | Dot Metrics Technology, Inc. | Quantum dot optoelectronic devices with nanoscale epitaxial lateral overgrowth and methods of manufacture |
US7988923B2 (en) | 2004-02-23 | 2011-08-02 | Rgf Environmental Group, Inc. | Device, system and method for an advanced oxidation process using photohydroionization |
US7403281B2 (en) | 2004-05-07 | 2008-07-22 | University Of Wyoming | Raman spectrometer |
US20050274663A1 (en) * | 2004-05-24 | 2005-12-15 | Roitman Lipa L | [Air and Water Conditioning System and Filter Media] |
US7993580B2 (en) * | 2004-08-24 | 2011-08-09 | Baxter International Inc. | Methods for the inactivation of microorganisms in biological fluids, flow through reactors and methods of controlling the light sum dose to effectively inactivate microorganisms in batch reactors |
US20080142435A1 (en) * | 2005-01-26 | 2008-06-19 | Sumitomo Electric Industries, Ltd. | Surface Emitting Device |
WO2006126584A1 (en) * | 2005-05-26 | 2006-11-30 | Matsushita Electric Industrial Co., Ltd. | Refrigerator |
US7259846B2 (en) | 2005-08-30 | 2007-08-21 | Agilent Technologies, Inc. | Lab in a cuvette |
JP4195499B2 (en) | 2005-10-11 | 2008-12-10 | 有限会社K2R | Photocatalytic reaction water generator |
US8203124B2 (en) | 2007-04-27 | 2012-06-19 | Hand Held Products, Inc. | Sterilization apparatus |
US7759854B2 (en) | 2007-05-30 | 2010-07-20 | Global Oled Technology Llc | Lamp with adjustable color |
WO2009021108A1 (en) * | 2007-08-07 | 2009-02-12 | Lee Antimicrobial Solutions Llc | Uv air treatment method and device |
CN102281933A (en) | 2008-12-19 | 2011-12-14 | 北卡罗来纳大学夏洛特分校 | Systems and methods for performing the bacterial disinfection of a fluid using point radiation sources |
WO2011103269A1 (en) | 2010-02-17 | 2011-08-25 | Dot Metrics Technologies, Inc. | Radiation delivery systems for fluid and vessel decontamination |
EP2683415B1 (en) | 2011-03-11 | 2017-06-14 | Deutsches Rheuma-Forschungszentrum Berlin | Flow cytometer disinfection module |
US20120246863A1 (en) | 2011-04-01 | 2012-10-04 | Douglas Ryan J | Control systems for uvc light source temperature and function in sanitizing device |
CN107187700A (en) | 2012-08-28 | 2017-09-22 | 传感器电子技术股份有限公司 | Food storage box and food deposit Tibetan device |
US11338048B2 (en) | 2012-12-11 | 2022-05-24 | Aquisense Technologies Llc | Apparatus for irradiation |
-
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11365134B2 (en) | 2019-07-31 | 2022-06-21 | Access Business Group International Llc | Water treatment system |
US11834353B2 (en) | 2019-07-31 | 2023-12-05 | Access Business Group International Llc | Water treatment system |
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US9976953B2 (en) | 2018-05-22 |
US20120318749A1 (en) | 2012-12-20 |
CA2747200C (en) | 2013-05-28 |
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CN102281933A (en) | 2011-12-14 |
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JP2012512723A (en) | 2012-06-07 |
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KR101280571B1 (en) | 2013-07-02 |
KR20110127115A (en) | 2011-11-24 |
CN105963731A (en) | 2016-09-28 |
WO2010071814A1 (en) | 2010-06-24 |
EP2379193B1 (en) | 2018-06-20 |
US20140240695A1 (en) | 2014-08-28 |
US20180008741A9 (en) | 2018-01-11 |
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